Malate dehydrogenase

Overview
NameMalate dehydrogenase
Smed IDSMED30011468
Length (bp)1094
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Malate dehydrogenase (SMED30011468) t-SNE clustered cells

Violin plots show distribution of expression levels for Malate dehydrogenase (SMED30011468) in cells (dots) of each of the 12 neoblast clusters.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011468

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30011468h1SMcG0017258 SmedASXL_007499SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
X1 cellSMED30011468h1SMcG0017258 SmedASXL_007499SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
testisSMED30011468h1SMcG0017258 Contig3666_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism adult hermaphrodite colorimetric in situ hybridization evidence
early male germ cellSMED30011468h1SMcG0017258 Contig3666_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism asexual adult cDNA to DNA expression microarray evidence
testis primordiumSMED30011468h1SMcG0017258 Contig3666_SE3GPL10652PMID:20844018
Wang et al., 2010		whole organism juvenile stagecDNA to DNA expression microarray evidence
neoblastSMED30011468h1SMcG0017258 SMED_08782_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30011468h1SMcG0017258 SMED_08782_V2_1GPL14150PMID:22385657
Wagner et al., 2012		whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH1 (malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970])

HSP 1 Score: 405.986 bits (1042), Expect = 1.983e-141
Identity = 205/310 (66.13%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH1 (malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970])

HSP 1 Score: 405.986 bits (1042), Expect = 1.983e-141
Identity = 205/310 (66.13%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH1 (malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970])

HSP 1 Score: 405.601 bits (1041), Expect = 4.823e-141
Identity = 205/310 (66.13%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH1 (malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970])

HSP 1 Score: 280.796 bits (717), Expect = 8.263e-94
Identity = 135/201 (67.16%), Postives = 159/201 (79.10%), Query Frame = 2 
Query:   38 SAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKL 640
            S PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL
Sbjct:   20 SEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL 220
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Human
Match: MDH1 (malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGNC:6970])

HSP 1 Score: 247.669 bits (631), Expect = 4.995e-81
Identity = 128/185 (69.19%), Postives = 144/185 (77.84%), Query Frame = 2 
Query:  410 VIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            VIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    2 VIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 185
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 354.755 bits (909), Expect = 1.183e-121
Identity = 186/310 (60.00%), Postives = 227/310 (73.23%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPS-IPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MSAP+ VL+TGAAGQIGYS+V  IA+G+VFG++Q V L L D+P     L+G+V EL+DC+ PTL S++A  D K  FT ID+A +VGAMPR++GMER+DLL ANV IFK QG+ L +YAK T KVIVVGNPANTN  I +KYA   IP +NFSA+TRLDHNRA +Q+ALK G    +V  V+IWGNHSGTQ+PDVT+  V   NG E     AV ++ +LQ  F+ TVQ RG  +I  RKLSSAMSAAKA CDH  DW  GT    +VSMAV SDGSY  P+ +IFSFPVTI+ G+W IV GL  DDF+
Sbjct:    1 MSAPLRVLVTGAAGQIGYSIVIRIADGTVFGKEQPVELVLLDVPQCSNILEGVVFELQDCALPTLFSVVAVTDEKSAFTGIDYAFLVGAMPRREGMERKDLLAANVKIFKSQGKALAEYAKPTTKVIVVGNPANTNAFIAAKYAAGKIPAKNFSAMTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVN-KNGTETDAYAAVGDNAFLQGPFIATVQKRGGVIIEKRKLSSAMSAAKAACDHIHDWHFGTKAGQFVSMAVPSDGSYGIPQGLIFSFPVTIEGGEWKIVQGLSFDDFA 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 302.368 bits (773), Expect = 5.743e-102
Identity = 158/263 (60.08%), Postives = 193/263 (73.38%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAP-SIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT 820
            MSAP+ VL+TGAAGQIGYS+V  IA+G+VFG++Q V L L D+P     L+G+V EL+DC+ PTL S++A  D K  FT ID+A +VGAMPR++GMER+DLL ANV IFK QG+ L +YAK T KVIVVGNPANTN  I +KYA   IP +NFSA+TRLDHNRA +Q+ALK G    +V  V+IWGNHSGTQ+PDVT+  V   NG E     AV ++ +LQ  F+ TVQ RG  +I  RKLSSAMSAAKA CDH  DW  GT
Sbjct:    1 MSAPLRVLVTGAAGQIGYSIVIRIADGTVFGKEQPVELVLLDVPQCSNILEGVVFELQDCALPTLFSVVAVTDEKSAFTGIDYAFLVGAMPRREGMERKDLLAANVKIFKSQGKALAEYAKPTTKVIVVGNPANTNAFIAAKYAAGKIPAKNFSAMTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVN-KNGTETDAYAAVGDNAFLQGPFIATVQKRGGVIIEKRKLSSAMSAAKAACDHIHDWHFGT 262
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Celegans
Match: mdh-1 (Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc:Q8IA49])

HSP 1 Score: 162.155 bits (409), Expect = 1.953e-48
Identity = 90/155 (58.06%), Postives = 108/155 (69.68%), Query Frame = 2 
Query:  497 LTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            +TRLDHNRA +Q+ALK G    +V  V+IWGNHSGTQ+PDVT+  V   NG E     AV ++ +LQ  F+ TVQ RG  +I  RKLSSAMSAAKA CDH  DW  GT    +VSMAV SDGSY  P+ +IFSFPVTI+ G+W IV GL  DDF+
Sbjct:    1 MTRLDHNRALAQLALKTGTTIGNVKNVIIWGNHSGTQFPDVTHATVN-KNGTETDAYAAVGDNAFLQGPFIATVQKRGGVIIEKRKLSSAMSAAKAACDHIHDWHFGTKAGQFVSMAVPSDGSYGIPQGLIFSFPVTIEGGEWKIVQGLSFDDFA 154
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Fly
Match: Mdh1 (gene:FBgn0262782 transcript:FBtr0345435)

HSP 1 Score: 379.022 bits (972), Expect = 3.962e-131
Identity = 192/309 (62.14%), Postives = 237/309 (76.70%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI V++TGAAGQI YSL+  IA G VFG+ Q ++LHL DIP MV  L+G+VMEL DC+ P L  ++ T D   GF D+  A +VGAMPRK+GMER+DLL ANV IF+ QG+ LDK+AKK VKV+VVGNPANTN L+ S YAPSIP+ENFSA+TRLD NRA SQIA K+GV  + V  ++IWGNHS TQYPD     V  ANG   SV +A+ ++G+LQ  F++TVQ RGAAVIAARK+SSAMSAAKA CDH  DW+ GT+   +VSM V+SDGSY+SPKD+IFSFPV IKN +W IV+GL + DF+
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAMPRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPANTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKVT-ANGTVKSVVDAINDNGYLQGSFVETVQKRGAAVIAARKMSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNKQWKIVSGLTLSDFA 308
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Fly
Match: Mdh1 (gene:FBgn0262782 transcript:FBtr0080050)

HSP 1 Score: 379.022 bits (972), Expect = 3.962e-131
Identity = 192/309 (62.14%), Postives = 237/309 (76.70%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI V++TGAAGQI YSL+  IA G VFG+ Q ++LHL DIP MV  L+G+VMEL DC+ P L  ++ T D   GF D+  A +VGAMPRK+GMER+DLL ANV IF+ QG+ LDK+AKK VKV+VVGNPANTN L+ S YAPSIP+ENFSA+TRLD NRA SQIA K+GV  + V  ++IWGNHS TQYPD     V  ANG   SV +A+ ++G+LQ  F++TVQ RGAAVIAARK+SSAMSAAKA CDH  DW+ GT+   +VSM V+SDGSY+SPKD+IFSFPV IKN +W IV+GL + DF+
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLYMIARGEVFGKDQPIVLHLLDIPPMVGVLEGVVMELADCALPLLVEVVPTTDPAVGFKDVSAAFLVGAMPRKEGMERKDLLSANVKIFRTQGQALDKFAKKDVKVLVVGNPANTNALVCSSYAPSIPRENFSAMTRLDQNRATSQIAAKLGVPISAVKNIIIWGNHSSTQYPDAGQAKVT-ANGTVKSVVDAINDNGYLQGSFVETVQKRGAAVIAARKMSSAMSAAKAACDHMHDWWNGTAPGQFVSMGVFSDGSYDSPKDVIFSFPVEIKNKQWKIVSGLTLSDFA 308
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 406.757 bits (1044), Expect = 7.290e-142
Identity = 199/310 (64.19%), Postives = 242/310 (78.06%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DI  M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QGE L+KYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V IWGNHS TQYPDV + +V   NGKE++  +AV ++ WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  WVSM +YS G SY  P D+++SFPV IKN  W +V GL I+DFS
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGEALEKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVPSDSVKNVTIWGNHSSTQYPDVHHAIVT-RNGKEIAAFDAVNDESWLKGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEWVSMGIYSSGNSYGVPDDLMYSFPVKIKNKSWKVVDGLSINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 405.986 bits (1042), Expect = 1.908e-141
Identity = 198/307 (64.50%), Postives = 240/307 (78.18%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DI  M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QGE L+KYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V IWGNHS TQYPDV + +V   NGKE++  +AV ++ WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  WVSM +YS G SY  P D+++SFPV IKN  W +V GL I+DFS
Sbjct:   21 PIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGEALEKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVPSDSVKNVTIWGNHSSTQYPDVHHAIVT-RNGKEIAAFDAVNDESWLKGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEWVSMGIYSSGNSYGVPDDLMYSFPVKIKNKSWKVVDGLSINDFS 326
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh1ab (malate dehydrogenase 1Ab, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-030131-7655])

HSP 1 Score: 380.563 bits (976), Expect = 1.329e-131
Identity = 191/310 (61.61%), Postives = 238/310 (76.77%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+  IA G VFG+ Q ++L L DI  M+P L+G+VMEL+DC+ P L+ +I T      F D+D A++VG+MPR++GMER+DLLKANV+IFK QGE LDKYAKKTVKV+VVGNPANTNCLI +K APSIPKENFS LTRLDHNRA SQ+AL+ G+   +V  V+IWGNHS TQYPDV +  V +  GK+ +  +AVK+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAI    +D + GT   +++SM VYS G SY  P+D+I+SFP++IK+  W IV GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLVLLLLDITPMLPVLEGVVMELQDCALPLLREVIPTDKEDVAFKDLDAAILVGSMPRREGMERKDLLKANVAIFKSQGEALDKYAKKTVKVLVVGNPANTNCLIAAKSAPSIPKENFSCLTRLDHNRASSQVALRCGIAPNNVKNVIIWGNHSSTQYPDVHHCKVNV-QGKDETAFDAVKDDAWLKGEFISTVQQRGAAVIKARKLSSAMSAAKAI---*QDIWTGTPEGEFISMGVYSTGNSYGVPEDLIYSFPISIKDKNWKIVDGLPINDFS 306
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BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 117.857 bits (294), Expect = 3.536e-32
Identity = 53/95 (55.79%), Postives = 71/95 (74.74%), Query Frame = 2 
Query:   38 SAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGM 322
            + PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DI  M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GM
Sbjct:   19 AEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDITPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGM 113
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BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Match: mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:NCBI gene;Acc:767794])

HSP 1 Score: 111.309 bits (277), Expect = 9.496e-27
Identity = 81/312 (25.96%), Postives = 142/312 (45.51%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNP-ANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVT-------NGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDD 949
            P+H+ I+ A   + YSL+ ++    +F       LHL D       L+ L ME  D + P L      G+  +   D            KD +            F   G+ ++  A+K V+V+V G+   N  C +L + APSI   NF A+T      A++Q+A K+ V  +D+  V++WGN SG+ + D+        +G ++  +G    V E + +  WL+ +F   +    A + +    ++A+S   AI    + W    S  +  S+ V S G +  P  ++FS PV+  +G W++ + + + D
Sbjct:  138 PLHIWISSALNPVCYSLIPHLFTPGLFSGLPIFSLHLMDTSGSEEMLQALKMETVDLAIPRLHE--PAGEYNDEQND------------KDQVAEH---------FHRYGQLIETNAQKDVRVLVAGDFFINMKCSLLIENAPSIDPRNFVAMTTQLEYEARTQLAQKLSVKTSDITNVIVWGNISGSFHIDLQRAKVFRYDGAIRGPDGFSQHVMEMIYDWKWLKTDFRSLLHKHRATISSKTNKATAISTTNAILAILKAWNNAESPEEVFSLGVLSTGQFGIPAGLVFSMPVSFTDGHWSVRSDVIVTD 426
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BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 394.815 bits (1013), Expect = 4.343e-137
Identity = 199/310 (64.19%), Postives = 236/310 (76.13%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M  P+ VL+TGAAGQI YSL+  IA G VFG+ Q +IL L DI  M+  L+G+VMEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA+ QIALK+ V   DV  V+IWGNHS TQYPD ++  V L  GK+V   EAVKND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM V SDG SY  P+D+++SFP+TIKN  W IV GL I+DFS
Sbjct:    1 MPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNL-QGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 392.889 bits (1008), Expect = 2.552e-136
Identity = 198/307 (64.50%), Postives = 235/307 (76.55%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            P+ VL+TGAAGQI YSL+  IA G VFG+ Q +IL L DI  M+  L+G+VMEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA+ QIALK+ V   DV  V+IWGNHS TQYPD ++  V L  GK+V   EAVKND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM V SDG SY  P+D+++SFP+TIKN  W IV GL I+DFS
Sbjct:    5 PVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNL-QGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFS 310
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 392.889 bits (1008), Expect = 3.067e-136
Identity = 198/310 (63.87%), Postives = 236/310 (76.13%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            +  P+ VL+TGAAGQI YSL+  IA G VFG+ Q +IL L DI  M+  L+G+VMEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA+ QIALK+ V   DV  V+IWGNHS TQYPD ++  V L  GK+V   EAVKND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM V SDG SY  P+D+++SFP+TIKN  W IV GL I+DFS
Sbjct:    7 LPEPVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNL-QGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFS 315
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 392.504 bits (1007), Expect = 6.273e-136
Identity = 198/307 (64.50%), Postives = 235/307 (76.55%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            P+ VL+TGAAGQI YSL+  IA G VFG+ Q +IL L DI  M+  L+G+VMEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA+ QIALK+ V   DV  V+IWGNHS TQYPD ++  V L  GK+V   EAVKND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM V SDG SY  P+D+++SFP+TIKN  W IV GL I+DFS
Sbjct:   20 PVKVLVTGAAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNL-QGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFS 325
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BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:448318])

HSP 1 Score: 379.407 bits (973), Expect = 4.798e-131
Identity = 192/299 (64.21%), Postives = 228/299 (76.25%), Query Frame = 2 
Query:   68 AAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            +AGQI YSL+  IA G VFG+ Q +IL L DI  M+  L+G+VMEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA+ QIALK+ V   DV  V+IWGNHS TQYPD ++  V L  GK+V   EAVKND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM V SDG SY  P+D+++SFP+TIKN  W IV GL I+DFS
Sbjct:    9 SAGQIAYSLLYGIAKGDVFGKDQPLILVLLDITPMMTVLEGVVMELQDCALPLLKEVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALNKYSKKSVKVIVVGNPANTNCLTAMKSAPSIPKENFSCLTRLDHNRAKGQIALKLNVASDDVKNVIIWGNHSSTQYPDASHATVNL-QGKDVGAFEAVKNDDWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPDGQFVSMGVISDGNSYGIPEDLMYSFPLTIKNKTWKIVEGLPINDFS 306
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 400.979 bits (1029), Expect = 1.383e-139
Identity = 203/310 (65.48%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P L+ +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA+SQIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAI DH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 400.979 bits (1029), Expect = 1.383e-139
Identity = 203/310 (65.48%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P L+ +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA+SQIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAI DH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 400.979 bits (1029), Expect = 3.020e-139
Identity = 203/310 (65.48%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P L+ +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA+SQIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAI DH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAIADHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh1 (malate dehydrogenase 1, NAD (soluble) [Source:MGI Symbol;Acc:MGI:97051])

HSP 1 Score: 211.075 bits (536), Expect = 1.303e-67
Identity = 101/155 (65.16%), Postives = 123/155 (79.35%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSAL 499
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P L+ +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS L
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCL 155
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Mouse
Match: Mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:MGI Symbol;Acc:MGI:1923918])

HSP 1 Score: 125.946 bits (315), Expect = 1.036e-31
Identity = 86/310 (27.74%), Postives = 154/310 (49.68%), Query Frame = 2 
Query:   41 APIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVG-NPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNG-------LVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLK 940
            +P+ V I  A   +   L+  + +G VFG   ++ L LFD       L+ +VME +D + P L+++      KE F      +++     ++       L++ V + ++ G  ++K A K+VKVIV G N  N    +L +YAP+I   N  A+      +A++ +A K+     ++  V+IWGN +G  Y D+           VK   G   SV   + +  W+ +EF++T++     + +  K    + AA +I    + W+ G+   + VS+ V S+G ++ P+ I+FS PV  +NG W ++T L+
Sbjct:  130 SPLQVWIASAGTYVCCHLIPLLLSGEVFGMHTEISLTLFDQEQREDCLRSIVMETQDLASPVLRTVSFCTTVKEAFLQAQVIIILDDSTEEEVYSLESCLRSRVPLCRLYGYLIEKNAHKSVKVIVGGKNFVNLKTTLLMQYAPNIAS-NIIAVALGVEGQAKAVLARKMKTTSANIKDVIIWGNITGNNYVDLRKAKVYNYESAVKGPPGHYHSVLSLIFDREWITKEFVQTLKI----LSSTGKQFGGILAAHSIATTLKYWYHGSPPGEIVSLGVMSEGQFDIPEGIVFSMPVKFENGTWVVLTDLE 434
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|Q3T145|MDHC_BOVIN (Malate dehydrogenase, cytoplasmic OS=Bos taurus OX=9913 GN=MDH1 PE=2 SV=3)

HSP 1 Score: 408.297 bits (1048), Expect = 1.037e-141
Identity = 205/310 (66.13%), Postives = 243/310 (78.39%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT   +  F D+D A++VG+MPR+DGMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM + SDG SY  P D+++SFPVTIK+  W +V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEIAFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGIISDGNSYGIPDDLLYSFPVTIKDKTWKVVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|P11708|MDHC_PIG (Malate dehydrogenase, cytoplasmic OS=Sus scrofa OX=9823 GN=MDH1 PE=1 SV=4)

HSP 1 Score: 408.297 bits (1048), Expect = 1.083e-141
Identity = 206/310 (66.45%), Postives = 242/310 (78.06%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT   +  F D+D A++VG+MPR+DGMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL   KEV V EAVK+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM + SDG SY  P D+++SFPVTIK+  W IV GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEIAFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVKL-QAKEVGVYEAVKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGIISDGNSYGVPDDLLYSFPVTIKDKTWKIVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|P40925|MDHC_HUMAN (Malate dehydrogenase, cytoplasmic OS=Homo sapiens OX=9606 GN=MDH1 PE=1 SV=4)

HSP 1 Score: 405.986 bits (1042), Expect = 9.522e-141
Identity = 205/310 (66.13%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR++GMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|Q7YRU4|MDHC_FELCA (Malate dehydrogenase, cytoplasmic OS=Felis catus OX=9685 GN=MDH1 PE=2 SV=3)

HSP 1 Score: 404.445 bits (1038), Expect = 3.345e-140
Identity = 202/310 (65.16%), Postives = 243/310 (78.39%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDGS-YNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT      F D+D A++VG+MPR+DGMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL   K APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV++  VKL +GKEV V +A+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM + SDG+ Y  P D+++SFPVTIKN  W +V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALEKYAKKSVKVIVVGNPANTNCLTACKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVSHAKVKL-HGKEVGVYDALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGIISDGNPYGVPDDLLYSFPVTIKNKTWKVVEGLTINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Match: sp|O88989|MDHC_RAT (Malate dehydrogenase, cytoplasmic OS=Rattus norvegicus OX=10116 GN=Mdh1 PE=1 SV=3)

HSP 1 Score: 400.979 bits (1029), Expect = 9.360e-139
Identity = 203/310 (65.48%), Postives = 240/310 (77.42%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MS PI VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P L+ +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA+SQIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+K+D WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAI DH RD + GT   ++VSM V SDG SY  P D+++SFPV IKN  W  V GL I+DFS
Sbjct:    1 MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLQDVIATDKEEVAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGAALEKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKDDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAISDHIRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A5N5JWU7 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00154770 PE=4 SV=1)

HSP 1 Score: 412.92 bits (1060), Expect = 4.420e-141
Identity = 204/310 (65.81%), Postives = 244/310 (78.71%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V+IWGNHS TQYPDV +  V L +GKEV+  EAVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM VYS G SY  P D+++SFPVTIKN  W +V  L I+DFS
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLFSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDRVKNVIIWGNHSSTQYPDVHHATVNL-HGKEVAAYEAVKDDSWLKGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSTGNSYGVPDDLMYSFPVTIKNKTWQVVDALPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A0P7W5L7 (Malate dehydrogenase OS=Scleropages formosus OX=113540 GN=Z043_124326 PE=3 SV=1)

HSP 1 Score: 410.223 bits (1053), Expect = 9.126e-140
Identity = 203/307 (66.12%), Postives = 245/307 (79.80%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            PI VL+TGAAGQI YSL+ +IANG VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  LDKYAKKTVKV+VVGNPANTNCLI +K APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  VVIWGNHS TQYPDV +  V++  GKEV V +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM +YS G SY  P D+IFSFP+TIKN  W IV GL+I+DFS
Sbjct:   15 PIKVLVTGAAGQIAYSLLYSIANGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALDKYAKKTVKVLVVGNPANTNCLIAAKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVPSDCVKDVVIWGNHSSTQYPDVHHAKVQM-QGKEVPVFDAVKDDAWLKGDFITTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPEGEFISMGIYSSGNSYGVPDDLIFSFPLTIKNKTWKIVDGLEINDFS 320
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: S7QD62 (Malate dehydrogenase OS=Myotis brandtii OX=109478 GN=D623_10034288 PE=3 SV=1)

HSP 1 Score: 409.838 bits (1052), Expect = 1.404e-139
Identity = 206/310 (66.45%), Postives = 242/310 (78.06%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M  P+ VL+TGAAGQI YSL+ +I NGSVFG+ Q +IL L DI  M+  L G++MEL+DC+ P LK +IAT   +  F D+D A++VG+MPR+DGMER+DLLKANV IFK QG  LDKYAKK+VKVIVVGNPANTNCL  SK APSIPKENFS LTRLDHNRA++QIALK+GV   DV  V+IWGNHS TQYPDV +  VKL  GKEV V EA+KND WL+ EF+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   ++VSM + SDG SY  P D+++SFPVTIKN  W IV GL I+DFS
Sbjct:   19 MPEPVRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEEVAFKDLDVAVLVGSMPRRDGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSEDVKNVIIWGNHSSTQYPDVNHAKVKL-QGKEVGVYEALKNDSWLKGEFITTVQQRGAAVIKARKLSSAMSAAKAICDHIRDIWFGTPEGEFVSMGIISDGNSYGVPDDLLYSFPVTIKNKTWKIVEGLPINDFS 327
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A437DNS2 (Malate dehydrogenase OS=Oryzias javanicus OX=123683 GN=OJAV_G00008790 PE=3 SV=1)

HSP 1 Score: 409.068 bits (1050), Expect = 1.855e-139
Identity = 200/310 (64.52%), Postives = 243/310 (78.39%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI V++TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF DID A++VG+MPRK+GMER+DLLKANV+IFK QG+ LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA SQ+A++ GV    V  V+IWGNHS TQYPDV +  V L NG EVS  +AVKND WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VY+ G SY  P+D+I+SFP+ IKN  W +V GL ++DFS
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLYSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDSVKNVIIWGNHSSTQYPDVHHAKVNL-NGAEVSAYDAVKNDAWLRGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNKTWKVVDGLPVNDFS 309
[Close]
BLAST of Malate dehydrogenase vs. TrEMBL
Match: A0A3B4DZ11 (Malate dehydrogenase OS=Pygocentrus nattereri OX=42514 PE=3 SV=1)

HSP 1 Score: 408.683 bits (1049), Expect = 2.209e-139
Identity = 202/310 (65.16%), Postives = 244/310 (78.71%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   +  F D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V+IWGNHS TQYPDV + +V L +GKEV+  +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM VYS G SY  P D+++SFPV IKN  W +V GL I+DFS
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVAFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAIRVGVSSDKVKNVIIWGNHSSTQYPDVHHAMVDL-HGKEVAAFDAVKDDTWLKGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTLDGEFVSMGVYSTGNSYGVPDDLMYSFPVMIKNKSWKVVDGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh1aa (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103036835])

HSP 1 Score: 404.831 bits (1039), Expect = 2.585e-141
Identity = 200/310 (64.52%), Postives = 241/310 (77.74%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  L+KYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V+IWGNHS TQYPDV +  V   +GKEV   +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM VYS G SY    D+++SFPV IKN  W +V GL I+DFS
Sbjct:    1 MAEPIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALEKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDKVKNVIIWGNHSSTQYPDVHHATVN-QHGKEVPAYDAVKDDSWLKADFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSSGNSYGVADDLMYSFPVMIKNKSWKVVDGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh1aa (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103036835])

HSP 1 Score: 404.06 bits (1037), Expect = 1.206e-140
Identity = 199/307 (64.82%), Postives = 239/307 (77.85%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  L+KYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V+IWGNHS TQYPDV +  V   +GKEV   +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM VYS G SY    D+++SFPV IKN  W +V GL I+DFS
Sbjct:   21 PIRVLVTGAAGQIAYSLLYSIAKGDVFGKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALEKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDKVKNVIIWGNHSSTQYPDVHHATVN-QHGKEVPAYDAVKDDSWLKADFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSSGNSYGVADDLMYSFPVMIKNKSWKVVDGLPINDFS 326
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh1ab (malate dehydrogenase, cytoplasmic [Source:NCBI gene;Acc:103033607])

HSP 1 Score: 395.971 bits (1016), Expect = 9.135e-138
Identity = 193/310 (62.26%), Postives = 243/310 (78.39%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGS-YNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+  I VL+TGAAGQI YSL+ +IA G VFG  Q ++L L DIP M+P L+G+VMEL+DC+ P LK +I T   +  F D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  LDKYAKKTVKV+VVGNPANTNCL+ +K APSIPKENFS LTRLDHNRA+SQ+A++ G+   +V  V+IWGNHS TQYPDV + +V +  GK+V+  + VK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VYS G+ Y  P+D+I+SFPVTIK+  W IV GL I+DFS
Sbjct:    1 MAETIRVLVTGAAGQIAYSLLYSIAKGDVFGRDQPLVLVLLDIPPMLPVLEGVVMELQDCALPILKEVIPTDKEEVAFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKSQGAALDKYAKKTVKVLVVGNPANTNCLVAAKSAPSIPKENFSCLTRLDHNRARSQVAMRCGIPADNVKNVIIWGNHSSTQYPDVHHCMVSVG-GKDVAAFDMVKDDDWLKGDFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWNGTPEGEFISMGVYSTGNPYGVPEDLIYSFPVTIKDKSWKIVDGLAINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh1aa (malate dehydrogenase 1Aa, NAD (soluble) [Source:ZFIN;Acc:ZDB-GENE-040204-1])

HSP 1 Score: 362.844 bits (930), Expect = 4.681e-125
Identity = 179/280 (63.93%), Postives = 216/280 (77.14%), Query Frame = 2 
Query:  125 GEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSD--WVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
             + Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF D+D A++VG+MPRK+GMER+DLLKANV+IFK QG  L+KYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA+SQ+A++VGV    V  V+IWGNHS TQYPDV +  V   +GKEV   +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT D  +VSM VYS G SY    D+++SFPV IKN  W +V GL I+DFS
Sbjct:    4 AKDQPIILVLLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDLDAAILVGSMPRKEGMERKDLLKANVAIFKTQGAALEKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRARSQVAMRVGVSSDKVKNVIIWGNHSSTQYPDVHHATVN-QHGKEVPAYDAVKDDSWLKADFISTVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTPDGEFVSMGVYSSGNSYGVADDLMYSFPVMIKNKSWKVVDGLPINDFS 282
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Match: mdh1b (malate dehydrogenase 1B [Source:NCBI gene;Acc:103037214])

HSP 1 Score: 127.102 bits (318), Expect = 2.511e-32
Identity = 86/316 (27.22%), Postives = 143/316 (45.25%), Query Frame = 2 
Query:   47 IHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRK-----DGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNP-ANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTN-------GLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDD 949
             H+ ITGA     YSL+  +    +  +   + LHL D+     +L GL ME ED +   L  +    D    F   D  L +   P       +  ++  ++      F+  G  ++  A+K V+V+V G+   N  C +L + APS+    F A+T      A++Q+A K+ V  TD+  +++WGN SG+ + D+         G +    G    V E + +  WL+ +FL  V  R  A+      ++A SA   I      W    S  +  S+ V S G++  P  ++FS PV   NG W++ + + I+D
Sbjct:  132 FHIWITGALSPTCYSLIPKLFTTELIRDISTISLHLLDVNSSEESLLGLKMETEDLALSQLHEVTVHSDMTWAFQSADLILFLDDWPSNSETPVEQGDKDHMVSQVAERFRSYGRLVEANAQKDVRVMVAGDSYVNLKCSLLIENAPSVDPCRFVAMTTQLEYEARAQLAEKLSVKTTDITDIIVWGNISGSSHVDLQRAKVFRYKGSIWGPTGFSQQVLEMIYDKKWLETDFLSLVALRRRAISLKTNRTAANSATNGILAVLNAWSNCYSSREVFSLGVVSSGNFGVPAGLVFSVPVKFHNGDWSVCSDITIND 447
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BLAST of Malate dehydrogenase vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007050.1 (pep scaffold:Pmarinus_7.0:GL479360:144570:153551:-1 gene:ENSPMAG00000006369.1 transcript:ENSPMAT00000007050.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 220.32 bits (560), Expect = 6.222e-72
Identity = 103/167 (61.68%), Postives = 133/167 (79.64%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQI 535
            M+  + VL+TGAAGQI YSL+ +IA+G+VFG+ Q + L L DI  M+  L+G+VMEL+DC+ P LK +IAT   +  F D+D A++VG+MPR++GMER+DLLKANV IFK QG  L+KY+KKTVKV+VVGNPANTNCLI  K A +IP+ENF+ LTRLDHNRA SQ+
Sbjct:    1 MTGTVKVLVTGAAGQIAYSLLYSIASGNVFGKDQPISLVLLDITPMLGVLEGVVMELQDCALPLLKEVIATDKEEVAFKDLDAAILVGSMPRREGMERKDLLKANVRIFKSQGAALNKYSKKTVKVLVVGNPANTNCLIALKSALTIPRENFTCLTRLDHNRAVSQV 167
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Sea Lamprey
Match: mdh1b (malate dehydrogenase 1B, NAD (soluble) [Source:NCBI gene;Acc:767794])

HSP 1 Score: 106.686 bits (265), Expect = 8.801e-26
Identity = 82/315 (26.03%), Postives = 139/315 (44.13%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVG-NPANTNC-LILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGK------EVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWV--SMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDD 949
            +S P HV ITGA       L+  +  G + G    + + L        AL+ +  +  D +   L+ +    D +  F      +++  +    G E  D   A   +    G  L + A   V+V+V     AN    L+ +  AP +P+ NF AL+     RA+  +A K+ V    V  VV+WG+  G+ Y  ++   ++  +G          + E + +  WL+ EF   +Q RG+    A+   +  +   AI    R WF G+ D    S+AV S+G Y   + ++FS PV  + G W++V+ L + D
Sbjct:  129 LSRPFHVCITGANSACVPGLLWLLGTGELCGGSTGLSVCLLGKVGEQEALRCVRQDTLDMACAALRDVTIHSDPELAFDGAGLVIVLDNVAPGLGQEWSD---AGAPLCAAYGSALGRRAGPDVRVLVAAAKGANLRAQLLATAAAPHVPRRNFVALSTHLERRAKGLLAAKLKVNSAGVEDVVVWGDAGGSTYTQLSAARLRGYDGAVWGPGLTYPLLEVLNDREWLENEFPAALQARGSLQAKAQGYPATTALNAAIASTLRLWFHGSDDQQIHSLAVPSNGEYGLLQGLVFSLPVRFEGGDWSVVSDLSLTD 440
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Yeast
Match: MDH3 (Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle [Source:SGD;Acc:S000002236])

HSP 1 Score: 52.373 bits (124), Expect = 3.800e-8
Identity = 65/251 (25.90%), Postives = 104/251 (41.43%), Query Frame = 2 
Query:  266 FTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLI-------LSKYAPSIPKENFSALTRLDHNRAQSQIA-------LKVG--VGFTDVHR-VVIWGNHSG-TQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARK------LSSAMSAAKAICDHCRDWF--KGTSDWVSMAVYSDGSYNSPKDI---------IFSFPVTIKNG 913
             ++    L+   +PRK G+ R DL K N  I K     + K+A    +++V+ NP N+   I       + K+ P     N   +T LD  RA++ +         K+G     T +HR V + G HSG T  P +T+  +     K+              E F+  VQ  G  ++ A++      LS A + AK   +  R +   K  ++ +S  VY  G  N  K            FS P+ ++NG
Sbjct:   66 LSNAQVVLIPAGVPRKPGLTRDDLFKMNAGIVKSLVTAVGKFA-PNARILVISNPVNSLVPIAVETLKKMGKFKPG----NVMGVTNLDLVRAETFLVDYLMLKNPKIGQEQDKTTMHRKVTVIGGHSGETIIPIITDKSLVFQLDKQY-------------EHFIHRVQFGGDEIVKAKQGAGSATLSMAFAGAKFAEEVLRSFHNEKPETESLSAFVYLPGLKNGKKAQQLVGDNSIEYFSLPIVLRNG 298
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BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Match: EDO41218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S4V3])

HSP 1 Score: 385.185 bits (988), Expect = 5.134e-134
Identity = 187/309 (60.52%), Postives = 234/309 (75.73%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGT--SDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ P+ V ITGAAGQI YSL+ ++A G VFG  Q +ILHL DI  M+ AL G+V+EL+DC+ P L  ++AT DA++ FTDID A++VGAMPR++GMER+DLL AN  IF+ QG+ LDK AKKT K++VVGNPANTNCLI  ++APSIPKENFS LTRLD NR  SQIA ++ +    V  V+IWGNHS TQYPDV +G V   NG  V++++AVK+D +L  EF+ TVQ RGAA+I ARKLSSAMSAAKAICDH RDW+ GT   +WVSM V SDGSY   + +++SFPV I +  W IV GL I+DFS
Sbjct:    1 MAEPLRVCITGAAGQIAYSLLYSVAKGDVFGANQPIILHLLDITPMMGALGGVVLELQDCAIPLLHEVVATDDAEKAFTDIDVAILVGAMPRREGMERKDLLSANAKIFEAQGKALDKVAKKTCKILVVGNPANTNCLIAQRWAPSIPKENFSCLTRLDQNRGGSQIAGRLNLRVDQVKNVIIWGNHSATQYPDVNHGKV-CVNGNWVAIRDAVKDDDYLNGEFISTVQKRGAAIIKARKLSSAMSAAKAICDHMRDWWFGTREGEWVSMGVVSDGSYGIEEGLVYSFPVQICDRTWKIVQGLSINDFS 308
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Match: EDO45840 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRY2])

HSP 1 Score: 182.57 bits (462), Expect = 1.883e-53
Identity = 95/312 (30.45%), Postives = 169/312 (54.17%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGK---------EVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFK-GTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDD 949
            P+ V ++ A+G + Y ++ ++  G VFG +++V ++L D P+   AL+G+  E+EDC++P  + +  T D    F D    +++      +G ++++ L ++  +F+  G+ L+ +AK   KV+  G PAN +  I SK+APSI K+NF +L+R++ NRA+  IA ++ V    +  +++WGN     YPD ++  V   +G             V E V ++ WL  EF++ ++             +A+  + A+    +DW +  +SD  S+ V S+G Y+ P+ I FSFPV    G W +V GL + D
Sbjct:  131 PLRVCVSKASGPLAYGMLASLVQGEVFGFQEEVSIYLLDTPENQEALQGVAYEIEDCAWPLFRGVHITSDPAVAFKDASVVVLLDGKAINEGTDKKEYLLSHAKLFRDYGKALEAHAKPDCKVLTAGGPANFSTFIASKFAPSIQKKNFVSLSRIEENRAKGLIAKRLNVNTAGIKDLIVWGNPGFNHYPDASHARVDGFDGAIWGPHVPGFTRPVPEMVHDNKWLSGEFVEVLKKPSEG-------ENALMGSVAVKSLLQDWCQCASSDMWSLGVISEGWYDIPEGIAFSFPVKFTGGSWQVVEGLALSD 435
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 406.371 bits (1043), Expect = 1.133e-141
Identity = 199/316 (62.97%), Postives = 247/316 (78.16%), Query Frame = 2 
Query:   17 IQSIN*MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            +++I+  + PI V++TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF DID A++VG+MPRK+GMER+DLLKANV+IFK QG+ LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA SQ+A++ GV    V  V+IWGNHS TQYPDV +  V ++ G EVS  +AVKND WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VY+ G SY  P+D+I+SFP+ IKN  W +V GL I+DFS
Sbjct:   12 VRAISPQAEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVNVS-GSEVSAYDAVKNDAWLRGDFISTVQLRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNKTWKVVDGLPINDFS 326
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 405.601 bits (1041), Expect = 1.249e-141
Identity = 199/310 (64.19%), Postives = 243/310 (78.39%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI V++TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF DID A++VG+MPRK+GMER+DLLKANV+IFK QG+ LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA SQ+A++ GV    V  V+IWGNHS TQYPDV +  V ++ G EVS  +AVKND WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VY+ G SY  P+D+I+SFP+ IKN  W +V GL I+DFS
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVNVS-GSEVSAYDAVKNDAWLRGDFISTVQLRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNKTWKVVDGLPINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 403.29 bits (1035), Expect = 1.372e-140
Identity = 198/309 (64.08%), Postives = 242/309 (78.32%), Query Frame = 2 
Query:   38 SAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            + PI V++TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF DID A++VG+MPRK+GMER+DLLKANV+IFK QG+ LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA SQ+A++ GV    V  V+IWGNHS TQYPDV +  V ++ G EVS  +AVKND WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VY+ G SY  P+D+I+SFP+ IKN  W +V GL I+DFS
Sbjct:    4 AEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVNVS-GSEVSAYDAVKNDAWLRGDFISTVQLRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIKNKTWKVVDGLPINDFS 311
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdhb (cytosolic malate dehydrogenase B [Source:NCBI gene;Acc:100049475])

HSP 1 Score: 394.045 bits (1011), Expect = 5.624e-137
Identity = 193/310 (62.26%), Postives = 242/310 (78.06%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDGS-YNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI VL+TGAAGQI YSL+ +IA G VFG+ Q +IL L DI  M+P L+G+VMEL+DC+ P L+ +IAT   +  F D+D A++VG+MPRK+GMER+DLLKANV+IF+ QG  LDKYAKKTV V+VVGNPANTNCLI +K APSIPKENFS LTRLDHNRA SQ+A++ GV  T V  V+IWGNHS TQYPDV +  V +++G E++  +AVK+D WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VYS G+ Y  P+D+I+SFPV IK+  W IV  L I+DFS
Sbjct:    1 MAEPISVLVTGAAGQIAYSLLFSIAKGDVFGKDQPIILLLLDITPMLPVLEGVVMELQDCALPLLREVIATDKEEVAFKDLDAAILVGSMPRKEGMERKDLLKANVAIFRSQGAALDKYAKKTVHVLVVGNPANTNCLIAAKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVPATHVKNVIIWGNHSSTQYPDVHHCKVSMSSG-ELACFDAVKDDSWLKGDFIATVQQRGAAVIKARKLSSAMSAAKAICDHMRDIWSGTPEGEFISMGVYSTGNPYGVPEDLIYSFPVQIKDKAWKIVDSLAINDFS 309
[Close]
BLAST of Malate dehydrogenase vs. Ensembl Medaka
Match: mdh1 (malate dehydrogenase 1 [Source:NCBI gene;Acc:100302445])

HSP 1 Score: 386.726 bits (992), Expect = 1.952e-134
Identity = 190/294 (64.63%), Postives = 232/294 (78.91%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTS--DWVSMAVYSDG-SYNSPKDIIFSFPVTIK 907
            M+ PI V++TGAAGQI YSL+ +IA G VFG+ Q +IL L DIP M+P L G+VMEL+DC+ P L+ +I T   + GF DID A++VG+MPRK+GMER+DLLKANV+IFK QG+ LDKYAKKTVKV+VVGNPANTNCLI SK APSIPKENFS LTRLDHNRA SQ+A++ GV    V  V+IWGNHS TQYPDV +  V ++ G EVS  +AVKND WL+ +F+ TVQ RGAAVI ARKLSSAMSAAKAICDH RD + GT   +++SM VY+ G SY  P+D+I+SFP+ IK
Sbjct:    1 MAEPIRVVVTGAAGQIAYSLLFSIAKGDVFGKDQPIILILLDIPPMLPVLDGVVMELQDCALPLLREVIPTDKVEVGFKDIDAAILVGSMPRKEGMERKDLLKANVAIFKTQGQALDKYAKKTVKVLVVGNPANTNCLIASKSAPSIPKENFSCLTRLDHNRACSQVAMRCGVSSDKVKNVIIWGNHSSTQYPDVHHAKVNVS-GSEVSAYDAVKNDAWLRGDFISTVQLRGAAVIKARKLSSAMSAAKAICDHMRDIWFGTKEGEFISMGVYAAGNSYGIPEDLIYSFPIQIK 293
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000027913.1 (SMESG000027913.1)

HSP 1 Score: 634.795 bits (1636), Expect = 0.000e+0
Identity = 306/307 (99.67%), Postives = 306/307 (99.67%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNC ILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS
Sbjct:    1 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCFILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 307
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BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000056995.1 (SMESG000056995.1)

HSP 1 Score: 491.115 bits (1263), Expect = 1.828e-175
Identity = 233/307 (75.90%), Postives = 266/307 (86.64%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTGLKIDDFS 955
            M+ PI V++TGAAGQIGY LV ++A+G+VFG  Q + LHL DIP MVP+LKGL+MELEDC+FPTL S+IAT D KE FTD+D+ALMVGAMPRK+GMER+DLLKANV+IFKVQGE LDKYAKKT KV VVGNPANTNCL++SKYAPSIPKENFSALTRLDHNRAQ+QIA K GV    V R VIWGNHS TQYPDV +GLV   +GKEVS+K+AVK+D WL+ +F+KTVQTRGAAVIAARKLSSA+SAAKA+ DHCRDWF GT +WVSMAVYSDGSY  PKD+IFSFPVTI +GKW IV GL IDDFS
Sbjct:    1 MTPPITVVLTGAAGQIGYCLVHSVASGNVFGPHQPINLHLLDIPVMVPSLKGLIMELEDCAFPTLHSVIATDDLKEAFTDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTAKVCVVGNPANTNCLVMSKYAPSIPKENFSALTRLDHNRAQAQIAAKAGVPCDHVKRCVIWGNHSNTQYPDVNHGLVHGHDGKEVSIKDAVKDDTWLKNDFIKTVQTRGAAVIAARKLSSALSAAKAVADHCRDWFHGTKEWVSMAVYSDGSYGVPKDVIFSFPVTIDHGKWHIVQGLTIDDFS 307
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BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000027913.1 (SMESG000027913.1)

HSP 1 Score: 259.225 bits (661), Expect = 5.406e-87
Identity = 127/132 (96.21%), Postives = 127/132 (96.21%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNP 430
            MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVK I    P
Sbjct:    1 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKQICSFYP 132
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000042152.1 (SMESG000042152.1)

HSP 1 Score: 241.506 bits (615), Expect = 1.202e-77
Identity = 113/281 (40.21%), Postives = 180/281 (64.06%), Query Frame = 2 
Query:   44 PIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGKEVSVKEAVKNDGWLQEEFLKTVQTR--GAAVIAARKLSSAMSAAKAICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDI 880
            P+ ++++GAA      L+  I NG ++G+ Q +ILHL +    +P +  L MEL+DC+F  L+ I+ T +  E +T+++   M+     +  M + D+ + N+  FK   E +++YA K VK+I+   P NTNCL++SKYAP+IP EN + + RLD NRAQ+Q+A ++GV    + R++IWGNHS T++ D++  +VKL + KE+S+++A+KND W++  F+KT+QTR          K+S  MS AKAI DH  D + GT+DW++M VY + S  + K I
Sbjct:   11 PLKIVLSGAAHPASIFLIRYILNGEIYGKDQPIILHLLEGSSKLPLVMALEMELQDCAFFLLEKILVTSNKIEAYTNVNIIFMLSEKKDQKEMFKVDVFRYNLKHFKRHAEIINEYADKRVKIIMCSRPINTNCLVMSKYAPNIPVENITGIIRLDVNRAQAQLASELGVPCNTIRRIIIWGNHSDTKFTDISYAMVKLPDTKEMSIQDAIKNDNWIKNHFIKTIQTRTDDPNFGGTGKVSYYMSLAKAIMDHSYDMWNGTADWINMIVYREESEITSKQI 291
[Close]
BLAST of Malate dehydrogenase vs. Planmine SMEST
Match: SMESG000070444.1 (SMESG000070444.1)

HSP 1 Score: 108.997 bits (271), Expect = 9.864e-26
Identity = 87/324 (26.85%), Postives = 146/324 (45.06%), Query Frame = 2 
Query:   35 MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPALKGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRK----DGMERRDLLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPA----NTNCLILSKYAPSIPKENFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLVKLANGK-------EVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARK-LSSAMSAAKAICDHCRDWFKGTSDWV--SMAVYSDGSYNSPKDIIFSFPVTIK-NGKWTIVTGLKIDD 949
            +S P  V+I GA  +     V +++ G V     ++ +   D  + +P LK L M   D ++  ++SI  T + +  F D   +L+V    R     D  E    +KAN   ++  G +++KY    VKV++V   A    N N + + +    IPK N   L+R    + +S +A K+ V  ++VH  ++WGN  G  Y D  N  V   +G         +SV + V +  WL+ E    +  R   +      L + ++ A AI      W  G       S+ + S+G Y  P  + FS PVT+   G WT+V  +++ +
Sbjct:  128 LSTPTKVVIVGAVNEAVPHFVLSLSRGDVLNSNTELAIVFVDKTEHLPILKSLQMGTFDLAYRLVRSISITDNFEAAFADS--SLVVIFDDRNPSPADPDEYIPWMKANQEYYEYIGNFIEKYCSLDVKVLLVPIKAISYLNINLISIHRMLQRIPKFNVVGLSRTVEQQVRSVVARKLNVNPSNVHNNIVWGNIGGKMYIDTNNSRVHRYDGAVMGPEPFSLSVTKTVWDKKWLKNEMEPLILNRFQEISDLNNGLGTPLALASAITTFLGQWINGDEHGYIHSLVIASEGWYGVPDGMAFSMPVTLNPQGYWTVVQDVELSE 449
The following BLAST results are available for this feature:
BLAST of Malate dehydrogenase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
MDH11.983e-14166.13malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGN... [more]
MDH11.983e-14166.13malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGN... [more]
MDH14.823e-14166.13malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGN... [more]
MDH18.263e-9467.16malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGN... [more]
MDH14.995e-8169.19malate dehydrogenase 1 [Source:HGNC Symbol;Acc:HGN... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
mdh-11.183e-12160.00Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
mdh-15.743e-10260.08Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
mdh-11.953e-4858.06Malate dehydrogenase [Source:UniProtKB/TrEMBL;Acc... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mdh13.962e-13162.14gene:FBgn0262782 transcript:FBtr0345435[more]
Mdh13.962e-13162.14gene:FBgn0262782 transcript:FBtr0080050[more]
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BLAST of Malate dehydrogenase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh1aa7.290e-14264.19malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1aa1.908e-14164.50malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1ab1.329e-13161.61malate dehydrogenase 1Ab, NAD (soluble) [Source:ZF... [more]
mdh1aa3.536e-3255.79malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1b9.496e-2725.96malate dehydrogenase 1B, NAD (soluble) [Source:NCB... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh14.343e-13764.19malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh12.552e-13664.50malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh13.067e-13663.87malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh16.273e-13664.50malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
mdh14.798e-13164.21malate dehydrogenase 1 [Source:NCBI gene;Acc:44831... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Mdh11.383e-13965.48malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh11.383e-13965.48malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh13.020e-13965.48malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh11.303e-6765.16malate dehydrogenase 1, NAD (soluble) [Source:MGI ... [more]
Mdh1b1.036e-3127.74malate dehydrogenase 1B, NAD (soluble) [Source:MGI... [more]
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BLAST of Malate dehydrogenase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q3T145|MDHC_BOVIN1.037e-14166.13Malate dehydrogenase, cytoplasmic OS=Bos taurus OX... [more]
sp|P11708|MDHC_PIG1.083e-14166.45Malate dehydrogenase, cytoplasmic OS=Sus scrofa OX... [more]
sp|P40925|MDHC_HUMAN9.522e-14166.13Malate dehydrogenase, cytoplasmic OS=Homo sapiens ... [more]
sp|Q7YRU4|MDHC_FELCA3.345e-14065.16Malate dehydrogenase, cytoplasmic OS=Felis catus O... [more]
sp|O88989|MDHC_RAT9.360e-13965.48Malate dehydrogenase, cytoplasmic OS=Rattus norveg... [more]
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BLAST of Malate dehydrogenase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A5N5JWU74.420e-14165.81Uncharacterized protein OS=Pangasianodon hypophtha... [more]
A0A0P7W5L79.126e-14066.12Malate dehydrogenase OS=Scleropages formosus OX=11... [more]
S7QD621.404e-13966.45Malate dehydrogenase OS=Myotis brandtii OX=109478 ... [more]
A0A437DNS21.855e-13964.52Malate dehydrogenase OS=Oryzias javanicus OX=12368... [more]
A0A3B4DZ112.209e-13965.16Malate dehydrogenase OS=Pygocentrus nattereri OX=4... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh1aa2.585e-14164.52malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
mdh1aa1.206e-14064.82malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
mdh1ab9.135e-13862.26malate dehydrogenase, cytoplasmic [Source:NCBI gen... [more]
mdh1aa4.681e-12563.93malate dehydrogenase 1Aa, NAD (soluble) [Source:ZF... [more]
mdh1b2.511e-3227.22malate dehydrogenase 1B [Source:NCBI gene;Acc:1030... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSPMAT00000007050.16.222e-7261.68pep scaffold:Pmarinus_7.0:GL479360:144570:153551:-... [more]
mdh1b8.801e-2626.03malate dehydrogenase 1B, NAD (soluble) [Source:NCB... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MDH33.800e-825.90Peroxisomal malate dehydrogenase; catalyzes interc... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO412185.134e-13460.52Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO458401.883e-5330.45Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Malate dehydrogenase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
mdh11.133e-14162.97malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
mdh11.249e-14164.19malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
mdh11.372e-14064.08malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
mdhb5.624e-13762.26cytosolic malate dehydrogenase B [Source:NCBI gene... [more]
mdh11.952e-13464.63malate dehydrogenase 1 [Source:NCBI gene;Acc:10030... [more]
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BLAST of Malate dehydrogenase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000027913.10.000e+099.67SMESG000027913.1[more]
SMESG000056995.11.828e-17575.90SMESG000056995.1[more]
SMESG000027913.15.406e-8796.21SMESG000027913.1[more]
SMESG000042152.11.202e-7740.21SMESG000042152.1[more]
SMESG000070444.19.864e-2626.85SMESG000070444.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011468 ID=SMED30011468|Name=Malate dehydrogenase|organism=Schmidtea mediterranea sexual|type=transcript|length=1094bp
ATAAAACTGTCAATAAATTCAATCCATAAATTAAATGTCTGCACCAATAC
ATGTACTAATTACAGGAGCAGCTGGTCAGATTGGCTATTCTCTGGTAGGC
AACATTGCTAATGGCTCAGTTTTTGGCGAAAAACAGCAAGTTATTTTACA
TCTATTTGACATCCCAGACATGGTTCCTGCTCTGAAAGGATTAGTGATGG
AACTTGAGGATTGTTCGTTTCCGACTTTAAAAAGTATTATTGCTACTGGA
GATGCAAAAGAAGGGTTTACTGATATCGACTGGGCTTTGATGGTTGGTGC
AATGCCAAGAAAAGATGGAATGGAAAGAAGAGACTTGCTTAAAGCAAATG
TTTCAATATTCAAAGTGCAAGGAGAATATCTTGATAAGTATGCTAAAAAA
ACTGTGAAAGTGATTGTTGTTGGTAATCCAGCAAATACAAACTGTTTGAT
TTTAAGCAAATATGCTCCTTCTATCCCAAAGGAAAATTTCTCTGCGCTAA
CGAGACTTGATCACAATCGGGCTCAGTCACAGATAGCTTTAAAAGTTGGG
GTCGGTTTCACTGATGTCCACCGAGTTGTAATATGGGGTAATCACAGTGG
CACTCAATACCCAGATGTAACCAATGGCCTTGTAAAACTTGCAAATGGTA
AAGAGGTTAGTGTGAAAGAAGCAGTGAAAAATGATGGTTGGTTACAAGAA
GAATTTCTTAAAACAGTTCAAACAAGAGGAGCAGCTGTAATTGCTGCTAG
AAAATTGAGTAGTGCCATGTCTGCTGCTAAAGCGATTTGTGATCATTGCA
GAGACTGGTTTAAAGGAACTAGTGATTGGGTTTCCATGGCAGTGTATAGT
GATGGGTCATATAATTCTCCGAAAGATATCATATTTAGTTTTCCAGTAAC
CATAAAAAATGGAAAATGGACAATTGTTACAGGCTTAAAAATCGACGATT
TCTCCAAAGCTAAATTAGAAGCTACTTCCAAAGAATTAGAAGAGGAAAAG
AAAGAGGCTTTGGCAGCATGTGCGTGACAATTATCAGCAAAATTATAAAA
TTTTCTTTATTTATATCATATTTTTAGTTCATATAAAAGGTCAA
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protein sequence of SMED30011468-orf-1

>SMED30011468-orf-1 ID=SMED30011468-orf-1|Name=SMED30011468-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=331bp
MSAPIHVLITGAAGQIGYSLVGNIANGSVFGEKQQVILHLFDIPDMVPAL
KGLVMELEDCSFPTLKSIIATGDAKEGFTDIDWALMVGAMPRKDGMERRD
LLKANVSIFKVQGEYLDKYAKKTVKVIVVGNPANTNCLILSKYAPSIPKE
NFSALTRLDHNRAQSQIALKVGVGFTDVHRVVIWGNHSGTQYPDVTNGLV
KLANGKEVSVKEAVKNDGWLQEEFLKTVQTRGAAVIAARKLSSAMSAAKA
ICDHCRDWFKGTSDWVSMAVYSDGSYNSPKDIIFSFPVTIKNGKWTIVTG
LKIDDFSKAKLEATSKELEEEKKEALAACA*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000208testis
PLANA:0000419prepharyngeal region
PLANA:0000429neoblast
PLANA:0002030early male germ cell
PLANA:0002109X1 cell
PLANA:0003304testis primordium
Vocabulary: INTERPRO
TermDefinition
IPR010945Malate_DH_type2
IPR011274Malate_DH_NAD-dep_euk
IPR036291NAD(P)-bd_dom_sf
IPR001557L-lactate/malate_DH
IPR016040NAD(P)-bd_dom
IPR015955Lactate_DH/Glyco_Ohase_4_C
IPR022383Lactate/malate_DH_C
IPR001236Lactate/malate_DH_N
IPR001252Malate_DH_AS
Vocabulary: biological process
TermDefinition
GO:0019752carboxylic acid metabolic process
GO:0055114oxidation-reduction process
GO:0005975carbohydrate metabolic process
GO:0006108malate metabolic process
GO:0006099tricarboxylic acid cycle
Vocabulary: molecular function
TermDefinition
GO:0016616oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0003824catalytic activity
GO:0016491oxidoreductase activity
GO:0016615malate dehydrogenase activity
GO:0030060L-malate dehydrogenase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 308..328
NoneNo IPR availableGENE3DG3DSA:3.40.50.720coord: 1..154
e-value: 1.4E-58
score: 199.2
NoneNo IPR availablePANTHERPTHR23382:SF3MALATE DEHYDROGENASE, CYTOPLASMICcoord: 2..329
IPR022383Lactate/malate dehydrogenase, C-terminalPFAMPF02866Ldh_1_Ccoord: 156..327
e-value: 7.4E-41
score: 139.9
IPR001236Lactate/malate dehydrogenase, N-terminalPFAMPF00056Ldh_1_Ncoord: 6..152
e-value: 5.7E-36
score: 123.7
IPR001557L-lactate/malate dehydrogenasePIRSFPIRSF000102Lac_mal_DHcoord: 1..329
e-value: 5.7E-68
score: 227.0
IPR010945Malate dehydrogenase, type 2TIGRFAMTIGR01759TIGR01759coord: 3..325
e-value: 4.5E-148
score: 490.5
IPR010945Malate dehydrogenase, type 2PANTHERPTHR23382MALATE DEHYDROGENASEcoord: 2..329
IPR011274Malate dehydrogenase, NAD-dependent, cytosolicTIGRFAMTIGR01758TIGR01758coord: 7..327
e-value: 1.4E-154
score: 512.3
IPR011274Malate dehydrogenase, NAD-dependent, cytosolicCDDcd01336MDH_cytoplasmic_cytosoliccoord: 3..314
e-value: 0.0
score: 576.499
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalGENE3DG3DSA:3.90.110.10coord: 155..330
e-value: 1.8E-68
score: 231.6
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalSUPERFAMILYSSF56327LDH C-terminal domain-likecoord: 156..327
IPR001252Malate dehydrogenase, active sitePROSITEPS00068MDHcoord: 155..167
IPR036291NAD(P)-binding domain superfamilySUPERFAMILYSSF51735NAD(P)-binding Rossmann-fold domainscoord: 4..153