Otopetrin
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011465 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Otopetrin vs. TrEMBL
Match: A0A4S2LI03 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_008757 PE=4 SV=1) HSP 1 Score: 75.8702 bits (185), Expect = 1.987e-14 Identity = 33/59 (55.93%), Postives = 40/59 (67.80%), Query Frame = 3 Query: 36 FIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQ---NSSCHNHLWTPKQCLHL 203 + D +G+N+YLRLGAVGFGFGVM+ DGFKIA+ A+ SCHNH W P LH Sbjct: 388 YFSRDTEGVNLYLRLGAVGFGFGVMIFDGFKIAEPWEARLNDQESCHNHFWIPIHILHF 446
BLAST of Otopetrin vs. TrEMBL
Match: A0A3R7G764 (Uncharacterized protein OS=Clonorchis sinensis OX=79923 GN=CSKR_3215s PE=4 SV=1) HSP 1 Score: 75.8702 bits (185), Expect = 1.987e-14 Identity = 33/59 (55.93%), Postives = 40/59 (67.80%), Query Frame = 3 Query: 36 FIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQ---NSSCHNHLWTPKQCLHL 203 + D +G+N+YLRLGAVGFGFGVM+ DGFKIA+ A+ SCHNH W P LH Sbjct: 388 YFSRDTEGVNLYLRLGAVGFGFGVMIFDGFKIAEPWEARLNDQESCHNHFWIPIHILHF 446
BLAST of Otopetrin vs. TrEMBL
Match: A0A5K3EP08 (Uncharacterized protein OS=Mesocestoides corti OX=53468 PE=4 SV=1) HSP 1 Score: 75.485 bits (184), Expect = 2.545e-14 Identity = 35/67 (52.24%), Postives = 47/67 (70.15%), Query Frame = 3 Query: 6 INSFSMTMNKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNS--SCHNHLWTPKQCLH 200 + +FS + F DN+GIN+YLRLGAV FGFGVM+ DGF++ADQ RA+ +CH+ LW P +H Sbjct: 289 LGTFSKSNPPFT-QDNEGINLYLRLGAVVFGFGVMIMDGFRVADQFRAEKIIFACHSVLWIPVNLIH 354
BLAST of Otopetrin vs. TrEMBL
Match: A0A158RFB0 (Uncharacterized protein OS=Hydatigena taeniaeformis OX=6205 GN=TTAC_LOCUS9377 PE=4 SV=1) HSP 1 Score: 75.485 bits (184), Expect = 2.944e-14 Identity = 33/54 (61.11%), Postives = 42/54 (77.78%), Query Frame = 3 Query: 45 HDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNS--SCHNHLWTPKQCLH 200 D++GIN+YLRLG V FGFGV++ DGF++ADQ RA NS SCH+ LWTP +H Sbjct: 337 QDSEGINLYLRLGTVVFGFGVIIMDGFRVADQFRAVNSERSCHSILWTPLNAVH 390
BLAST of Otopetrin vs. TrEMBL
Match: G7YVK5 (Otopetrin OS=Clonorchis sinensis OX=79923 GN=CLF_111941 PE=4 SV=1) HSP 1 Score: 75.0998 bits (183), Expect = 4.001e-14 Identity = 33/59 (55.93%), Postives = 40/59 (67.80%), Query Frame = 3 Query: 36 FIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQ---NSSCHNHLWTPKQCLHL 203 + D +G+N+YLRLGAVGFGFGVM+ DGFKIA+ A+ SCHNH W P LH Sbjct: 309 YFSRDTEGVNLYLRLGAVGFGFGVMIFDGFKIAEPWEARLNDQESCHNHFWIPIHILHF 367
BLAST of Otopetrin vs. Planmine SMEST
Match: SMESG000018670.1 (SMESG000018670.1) HSP 1 Score: 130.183 bits (326), Expect = 2.913e-37 Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 3 Query: 30 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 203 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL Sbjct: 192 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 249
BLAST of Otopetrin vs. Planmine SMEST
Match: SMESG000018670.1 (SMESG000018670.1) HSP 1 Score: 130.183 bits (326), Expect = 2.940e-37 Identity = 58/58 (100.00%), Postives = 58/58 (100.00%), Query Frame = 3 Query: 30 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 203 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL Sbjct: 190 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 247
BLAST of Otopetrin vs. Planmine SMEST
Match: SMESG000018672.1 (SMESG000018672.1) HSP 1 Score: 105.145 bits (261), Expect = 3.439e-28 Identity = 45/58 (77.59%), Postives = 51/58 (87.93%), Query Frame = 3 Query: 30 NKFIFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 203 N ++ + +GIN+YLRLGAVGFGFGVMVHDGFK+ADQVR QNS CHN LWTPKQCLHL Sbjct: 188 NYYVIKETNGINLYLRLGAVGFGFGVMVHDGFKVADQVRLQNSFCHNQLWTPKQCLHL 245
BLAST of Otopetrin vs. Planmine SMEST
Match: SMESG000018671.1 (SMESG000018671.1) HSP 1 Score: 97.0561 bits (240), Expect = 2.196e-25 Identity = 41/55 (74.55%), Postives = 47/55 (85.45%), Query Frame = 3 Query: 39 IFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 203 I D++GIN+YLRLGAVGFGFGVMVHDGFKIAD+ R + +CHN LWTPK CLHL Sbjct: 171 IMRDHEGINLYLRLGAVGFGFGVMVHDGFKIADKFRLEGENCHNELWTPKHCLHL 225
BLAST of Otopetrin vs. Planmine SMEST
Match: SMESG000018671.1 (SMESG000018671.1) HSP 1 Score: 97.0561 bits (240), Expect = 2.389e-25 Identity = 41/55 (74.55%), Postives = 47/55 (85.45%), Query Frame = 3 Query: 39 IFHDNDGINVYLRLGAVGFGFGVMVHDGFKIADQVRAQNSSCHNHLWTPKQCLHL 203 I D++GIN+YLRLGAVGFGFGVMVHDGFKIAD+ R + +CHN LWTPK CLHL Sbjct: 198 IMRDHEGINLYLRLGAVGFGFGVMVHDGFKIADKFRLEGENCHNELWTPKHCLHL 252 The following BLAST results are available for this feature:
BLAST of Otopetrin vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Otopetrin vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Otopetrin vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Otopetrin vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Otopetrin vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Otopetrin vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Otopetrin vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Otopetrin vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011465 ID=SMED30011465|Name=Otopetrin|organism=Schmidtea mediterranea sexual|type=transcript|length=204bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|