SMED30011454

Overview
NameSMED30011454
Smed IDSMED30011454
Length (bp)1315
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011454 (SMED30011454) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011454 (SMED30011454) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011454

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011454h1SMcG0003462 SmedASXL_018909SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30011454h1SMcG0003462 SmedASXL_003112SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
muscle cellSMED30011454h1SMcG0003462 dd_Smed_v4_27160_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30011454h1SMcG0003462 dd_Smed_v6_27160_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011454 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.012720:10635..12002 -6498
Homology
BLAST of SMED30011454 vs. Planmine SMEST
Match: SMESG000080896.1 (SMESG000080896.1)

HSP 1 Score: 631.713 bits (1628), Expect = 0.000e+0
Identity = 360/360 (100.00%), Postives = 360/360 (100.00%), Query Frame = 1
Query:  136 DIETKISTRKLKPNPVRTYHHIPKYSKYKSEGEIIQCNSTFHLLHYVIYNPQKECEKYSSVVIGFEELMIFIHEIRSSSMPIGEMVFFLRNHIDKNTKFRVDYAEFHPNFNLEVSLRKSSMAHENHVVCVKFNCPVHESNNKDMICPAPSKPSVYTNDNKPYHGSWFKXXXXXXXXXXXXXXXXXXXXXXXFYPKIEKENKGISTYQKNPMIDYKTCNSFDAYDFLRKMDNIQSSLSETYAKPSNFHXXXXXXXXXXXXXXXXXXXXXXRIIVTEENNGNPNKTGYSINSSKTDHLELMNNKTKTLPLIKKNYNFDSSRSAPSPEYSLMDQDLSNVSSRTNPSFRSVKYIPPLKLVENEF 1215
            DIETKISTRKLKPNPVRTYHHIPKYSKYKSEGEIIQCNSTFHLLHYVIYNPQKECEKYSSVVIGFEELMIFIHEIRSSSMPIGEMVFFLRNHIDKNTKFRVDYAEFHPNFNLEVSLRKSSMAHENHVVCVKFNCPVHESNNKDMICPAPSKPSVYTNDNKPYHGSWFKIGISLIIILVLAIIAVLIYLIASFYPKIEKENKGISTYQKNPMIDYKTCNSFDAYDFLRKMDNIQSSLSETYAKPSNFHPSPNLSDNNNNNNTDTCKSSSNRIIVTEENNGNPNKTGYSINSSKTDHLELMNNKTKTLPLIKKNYNFDSSRSAPSPEYSLMDQDLSNVSSRTNPSFRSVKYIPPLKLVENEF
Sbjct:   21 DIETKISTRKLKPNPVRTYHHIPKYSKYKSEGEIIQCNSTFHLLHYVIYNPQKECEKYSSVVIGFEELMIFIHEIRSSSMPIGEMVFFLRNHIDKNTKFRVDYAEFHPNFNLEVSLRKSSMAHENHVVCVKFNCPVHESNNKDMICPAPSKPSVYTNDNKPYHGSWFKIGISLIIILVLAIIAVLIYLIASFYPKIEKENKGISTYQKNPMIDYKTCNSFDAYDFLRKMDNIQSSLSETYAKPSNFHPSPNLSDNNNNNNTDTCKSSSNRIIVTEENNGNPNKTGYSINSSKTDHLELMNNKTKTLPLIKKNYNFDSSRSAPSPEYSLMDQDLSNVSSRTNPSFRSVKYIPPLKLVENEF 380          
The following BLAST results are available for this feature:
BLAST of SMED30011454 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011454 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000080896.10.000e+0100.00SMESG000080896.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011454 ID=SMED30011454|Name=SMED30011454|organism=Schmidtea mediterranea sexual|type=transcript|length=1315bp
AAATAAAGAAAACCAAAAATTTTCGATCTGGATTAATTTTTTTGTGTATT
TTGAACTTTTATTTCAACGGTTATCATGAAGAATATTTTCTCAATTATAA
TTGCGTTGTTGTCGATTGCTGTCAGTGCTCAAATTGATATTGAAACGAAA
ATCTCTACAAGAAAATTGAAACCGAACCCGGTGCGGACATATCATCATAT
TCCTAAATATTCCAAATATAAAAGCGAAGGAGAAATCATACAGTGCAATT
CAACGTTTCATTTGTTGCATTATGTGATTTACAATCCCCAAAAAGAATGT
GAAAAATATTCCAGCGTCGTTATAGGATTTGAAGAATTGATGATATTTAT
CCACGAGATCAGATCGTCATCGATGCCAATTGGGGAAATGGTTTTTTTCT
TAAGAAATCACATTGATAAAAATACGAAATTCAGAGTTGATTATGCTGAA
TTTCATCCGAATTTTAATTTAGAAGTCAGTTTAAGAAAGTCCTCCATGGC
CCATGAGAATCATGTGGTTTGTGTAAAGTTCAACTGTCCCGTCCATGAGT
CTAACAATAAAGACATGATTTGTCCGGCTCCATCGAAACCATCAGTTTAT
ACCAATGACAATAAGCCGTATCATGGGAGTTGGTTCAAAATTGGAATTTC
TTTAATCATCATTCTAGTATTGGCAATAATAGCAGTTTTGATATATTTGA
TCGCATCATTTTATCCAAAAATAGAAAAAGAGAATAAAGGTATTTCAACT
TATCAAAAGAATCCAATGATAGATTATAAAACCTGCAATAGTTTTGACGC
TTATGATTTTCTACGGAAAATGGATAACATCCAATCAAGTCTATCAGAAA
CCTACGCAAAACCCAGCAATTTTCATCCTAGTCCCAACTTATCCGACAAT
AACAACAACAACAACACAGATACGTGTAAATCAAGCTCGAATCGCATTAT
CGTTACCGAAGAGAACAACGGAAACCCAAACAAAACCGGTTACAGTATCA
ATTCAAGCAAAACCGATCATTTGGAATTGATGAACAACAAAACAAAAACC
CTTCCTCTTATCAAAAAAAATTACAATTTTGATTCATCGAGATCAGCGCC
GTCGCCAGAATATTCATTGATGGATCAAGATCTGTCGAATGTCAGTTCCA
GGACTAATCCGTCGTTTCGATCTGTGAAATATATTCCTCCACTGAAATTA
GTAGAAAATGAATTTTAATCATTCACCTGACGAAAACAGAGAATTTGTAC
AAATGACAGAGATGAAGAAAGAAAATTATTAAGAAAAATTTTATTATTGT
TAAAAACTGTTAGAG
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protein sequence of SMED30011454-orf-1

>SMED30011454-orf-1 ID=SMED30011454-orf-1|Name=SMED30011454-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp
MKNIFSIIIALLSIAVSAQIDIETKISTRKLKPNPVRTYHHIPKYSKYKS
EGEIIQCNSTFHLLHYVIYNPQKECEKYSSVVIGFEELMIFIHEIRSSSM
PIGEMVFFLRNHIDKNTKFRVDYAEFHPNFNLEVSLRKSSMAHENHVVCV
KFNCPVHESNNKDMICPAPSKPSVYTNDNKPYHGSWFKIGISLIIILVLA
IIAVLIYLIASFYPKIEKENKGISTYQKNPMIDYKTCNSFDAYDFLRKMD
NIQSSLSETYAKPSNFHPSPNLSDNNNNNNTDTCKSSSNRIIVTEENNGN
PNKTGYSINSSKTDHLELMNNKTKTLPLIKKNYNFDSSRSAPSPEYSLMD
QDLSNVSSRTNPSFRSVKYIPPLKLVENEF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000418head
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.795
NoneNo IPR availableTMHMMTMhelixcoord: 189..211