PiggyBac transposable element-derived protein 4

Overview
NamePiggyBac transposable element-derived protein 4
Smed IDSMED30011417
Length (bp)2082
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of PiggyBac transposable element-derived protein 4 (SMED30011417) t-SNE clustered cells

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30011417) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of PiggyBac transposable element-derived protein 4 (SMED30011417) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for PiggyBac transposable element-derived protein 4 (SMED30011417) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011417

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymaSMED30011417SMESG000062192.1 SMESG000062191.1 dd_Smed_v4_12008_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011417SMESG000062192.1 SMESG000062191.1 dd_Smed_v4_12008_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: CABZ01072083.2 (dual specificity phosphatase 26 [Source:NCBI gene;Acc:570815])

HSP 1 Score: 81.6481 bits (200), Expect = 1.270e-15
Identity = 65/255 (25.49%), Postives = 111/255 (43.53%), Query Frame = 3 
Query:  384 NVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSKFYNIIVC----------------DIAKK---VIWLNLYLTWNQSKLNSVENVFEKD----LVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSDKRRRCYCCLYEKGRGFKQ------YCCVCQKPVFNEHSLKTVVC 1058
            N+  D  FTS+ L   L  + IT +GT R S+  +P   L  + R    + F      T+V+Y P  ++ V+++ST+H   A +   ++K ++ L YN+    VD+  K  +   C                D++     V+W++L  +W   K N    +F +D    LV P++QR+ +L  L + +  I E      +    I  V   KR+RC  C      G ++       C  C   +   H++ T +C
Sbjct:  331 NITCDQSFTSYALGEELLRRNITMIGTTRLSNPELPPALLSVKGRAINSSKFAFTDTHTLVSYHPRKNKNVLVMSTLHRDDAENSDEQSKPQMILDYNSTRGGVDNFDKLTSTYTCQRKTLHWPMVIFFNMLDVSAYNAFVLWMDLNPSWEAQKTNR-RRIFLQDLGRALVTPHLQRRERLPRLPNSINMIVEAQSAPEDIQRCIPPVTDPKRKRCEPCNRSTNGGKRRDTKTRLTCDKCNMYICKAHAVITTLC 584
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A1Y1MEP5 (DDE_Tnp_1_7 domain-containing protein (Fragment) OS=Photinus pyralis OX=7054 PE=4 SV=1)

HSP 1 Score: 148.673 bits (374), Expect = 1.854e-36
Identity = 91/263 (34.60%), Postives = 133/263 (50.57%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINLYNNRIHRVDDLSKFYN-------------------IIVCDIAKKVIWLNLYLTWNQSKLNSVENVF----EKDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPS-DKRRRCYCCLYEKGRGFKQYCCVCQKPVFNEHSLKTVVC 1058
            VVE+LC  F + NRN+  DNYFT   LA++LFSKG+T VGTVRK+   IP  FL  +   +  ++FG  +N T+V+Y P   + VIL+S+MH+T ++D  N     ++ YN     VD L +  +                   +  C +A  VIW  LY  WN+ K+N    +F     + LV P+I R+ +   L+ +   + +   N        +  P   K+RRC+ C  +K R  KQ C +C K V NEHS     C
Sbjct:   10 VVELLCQPFHHTNRNITCDNYFTDLSLAQSLFSKGLTMVGTVRKNKRFIPQKFLQTKRTNWETSIFGFTKNFTLVSYTPRPRKFVILLSSMHYTPSIDETNNKPEIVSYYNKTKGGVDLLDQLVHEYMSKRKCNRWPMAYFTSALDCCGVAAYVIWTKLYPQWNE-KVNYRRRLFLTSIAESLVKPHIYRRRECPGLQKRTRKLIDQFQNDKEVESARTNEPGPSKKRRCHICPAKKARTQKQICSLCNKNVCNEHSTTKRSC 271
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A4C1VNV0 (PiggyBac transposable element-derived protein 4 OS=Eumeta variegata OX=151549 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 146.747 bits (369), Expect = 2.945e-35
Identity = 91/230 (39.57%), Postives = 126/230 (54.78%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSK------------------FYNII-VCDIAKKVIWLNLYLTWNQSKLNSVENVFEK----DLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSDKRRRC 959
            VV  LC+ +   NRNV FDNYFTS ++A++L   G+T VGT+RK+   IP NF   + R     VFG   N+T+V+Y P  +R VI +STMHH    DV   NKSEINL YN+    VD L                    F NII V  IA  +IW N++  WN  K N++  +F +    +LV P+I+R+S   L    +AA+ ++    SN   T S+ P+ KR++C
Sbjct:   81 VVNQLCEPYFRSNRNVTFDNYFTSIDVAKSLAQNGLTIVGTLRKNKTCIPPNFQPKKTRHIESNVFGFYENITLVSYGPKKNRTVIFLSTMHHGKEEDVNKNNKSEINLYYNSTKGGVDTLDHMVHEYTVRRKTNRWPIAFFQNIIDVVGIASLIIWKNIHPDWNSHKKNTLRKLFLREIATELVTPHIRRRSTKGLSSYHLAAMQDVA--GSNQEATQSSEPAAKRKKC 308
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A4C1ZGA0 (PiggyBac transposable element-derived protein 4 OS=Eumeta variegata OX=151549 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 144.436 bits (363), Expect = 2.365e-33
Identity = 94/233 (40.34%), Postives = 128/233 (54.94%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSK------------------FYNIIVCDIAKKVIWLNLYLTWNQSKLNSVENVF----EKDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSDKR-RRCYCC 968
            VV  LC+ +   NRNV FDNYF S ++A++L   G+T VGT+RK+   IP NF   + R     VFG  +NMT+V+YVP  + AVIL+S MHH    DV   NKSEINL YN+    VD L +                  F NII   IA  +IW N++  WN  K N++  +F      +LV P+I+R+S   L    +AA+ ++    SN   T S+ P+ KR +RCY C
Sbjct:  222 VVNQLCEPYFRSNRNVTFDNYFMSIDVAKSLAQNGLTIVGTLRKNKTCIPPNFQPKKTRDIESNVFGFYKNMTLVSYVPKKNCAVILLSMMHHGKE-DVNKNNKSEINLYYNSTKGDVDTLDQMVHEYTVRRKTNRWPIAFFQNIIDVGIASLIIWKNIHPDWNSRKKNTLRILFLHEIATELVTPHIRRRSTKGLSIYHLAAMQDVA--GSNQEATHSSEPAAKRKKRCYIC 451
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A4C1UEE4 (PiggyBac transposable element-derived protein 4 OS=Eumeta variegata OX=151549 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 5.298e-29
Identity = 81/193 (41.97%), Postives = 110/193 (56.99%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSK------------------FYNII-VCDIAKKVIWLNLYLTWNQSKLNSVENVFEK----DLVVPNIQRKS 848
            V+  LC+     NRNV FDNYFTS ++A++L   G+T VGT+RK+   IP NF   + R     VFG  +NMT+V+YVP  + A+IL+STMHH    DV   NKSEINL YN+    VD L +                  F NII V  IA  +IW N++  WN  K N++  +F +    +LV P+I+R+S
Sbjct:  312 VLNQLCEPNIRSNRNVTFDNYFTSIDVAKSLAQNGLTIVGTLRKNKTCIPPNFQPKKTRDIESNVFGFYKNMTLVSYVPKKNHALILLSTMHHGKDEDVNKNNKSEINLYYNSTKGGVDTLDQMVHEYTVRRKTNRWPIAFFQNIIDVVGIASLIIWKNIHPDWNSRKKNTLRKLFLREIATELVTPHIRRRS 504
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A4C1U7C2 (PiggyBac transposable element-derived protein 4 OS=Eumeta variegata OX=151549 GN=PGBD4 PE=4 SV=1)

HSP 1 Score: 117.857 bits (294), Expect = 1.559e-24
Identity = 59/110 (53.64%), Postives = 72/110 (65.45%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINLY 671
            VV  LC  +   NRNV FDNYFTS ++A+ L   G+T VGT+RK+   IP NF   + R     VFG  +NMT+V+YVP  +RAVIL+STMHH    DV   NKSEINLY
Sbjct:  312 VVNQLCQPYFRSNRNVTFDNYFTSIDVAKYLAQNGLTTVGTLRKNKTCIPPNFQPKKTRDIESNVFGFYKNMTLVSYVPKKNRAVILLSTMHHGKEEDVNKNNKSEINLY 421
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000027888.1 (pep primary_assembly:ASM223467v1:17:9217998:9219211:-1 gene:ENSORLG00000025878.1 transcript:ENSORLT00000027888.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 82.4185 bits (202), Expect = 2.054e-16
Identity = 65/249 (26.10%), Postives = 113/249 (45.38%), Query Frame = 3 
Query:  384 NVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSK------------------FYNII-VCDIAKKVIWLNLYLTWNQSKLNSVENVFE---KDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSD--KRRRCYCCLYEKGRGFKQYCCVCQKPVFNEHSLKTVV 1055
            N+  DN+FTS+ L   L  + +  +GTVR++   +P   L  + R    ++F      T+V+Y P  ++ V+++STMH   ++  ++  K ++ L YN+    VD+L K                  F+NI+ V      V+W  +   WN  KL   +   E   K L+ P IQR+++     +  A +  I    SN +  +  V +D  KR+RC  C           C  C+  +  +H+  +V+
Sbjct:  130 NITCDNFFTSYRLGEELQKRKLIMLGTVRRNKPELPSEILNIQGRPMHSSIFAFTEKATVVSYCPKRNKNVLVMSTMHTDASLSTRDDMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTARWPMAIFFNIVDVSAYNAYVLWSEINQEWNAGKLYRRQLFLEELSKALITPKIQRRARPARSPAAAAVVKNIKFRPSNQS-AMDPVDTDVKKRKRCQVCPSRDDSKTSTSCAKCKNYICRKHTFHSVL 377
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000037833.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:101160710])

HSP 1 Score: 82.8037 bits (203), Expect = 4.719e-16
Identity = 66/245 (26.94%), Postives = 113/245 (46.12%), Query Frame = 3 
Query:  384 NVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINL-YNNRIHRVDDLSK------------------FYNII-VCDIAKKVIWLNLYLTWNQSKLNSVENVFE---KDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSD--KRRRCYCCLYEKGRGFKQYCCVCQKPVFNEHSL 1043
            N+  DN+FTS+ L   L  + +T +GTVR++   +P   L  + R    ++F      T+V+Y P  ++ V+++STMH   ++  ++  K ++ L YN+    VD+L K                  F+NI+ V      V+W  +   WN  KL       E   K L+ P IQR+++     +  A I++I   +SN    +  V +D  KR+RC  C           C  C+  +  +H++
Sbjct:  327 NITCDNFFTSYRLGEELQKRKLTMLGTVRRNKPELPSEILKIQGRPMHSSIFAFTEKATVVSYCPKRNKNVLVMSTMHTDASLSTRDDMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTARWPMAIFFNIVDVSAYNAYVLWSEINQEWNAGKLYRRRLFLEELGKALITPKIQRRARPARSPAAAAVIEKIKFRTSNQF-AMDPVDTDVKKRKRCQVCPSRDDSKTSTSCVKCKNFICRKHTV 570
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000029842.1 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101157644])

HSP 1 Score: 72.0182 bits (175), Expect = 1.412e-12
Identity = 60/225 (26.67%), Postives = 95/225 (42.22%), Query Frame = 3 
Query:  378 NRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAV--DVQNKNKSEINLYNNRIHRVDDLSKFYNIIVCDIAKK-------------------VIWLNL---YLTWNQSKLNSVENVFEKDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSDKRRRCYCCLYEK 980
            +RNV  DN+FTS+ LA  L  + IT +GT++ +   +P      R R+   + F        ++YVP  ++ V+L+ST H    +  + ++K    I  YN     VD+L K      C    K                   VIW  +   ++ WN+++         KDLV P + R+ ++   +    A D  L+ S       SA    KR+RC  C   K
Sbjct:  576 HRNVTCDNFFTSYALATELLKRNITLLGTIKSNKPELPPELASARGREALSSRFAFTDCAAALSYVPKKNKNVLLLSTKHSRVEICHERRDKKPEMILDYNKTKGGVDNLDKLLATYSCRRMTKRWPLAMFHNMIDMSAYNAYVIWREMHPDWMPWNRNRRRIFLEQLGKDLVRPLVLRRKRVPRAR---PAYD--LMVSMQEAGGTSARSEGKRKRCQICPRSK 795
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000062192.1 (SMESG000062192.1)

HSP 1 Score: 265.774 bits (678), Expect = 1.530e-85
Identity = 131/133 (98.50%), Postives = 132/133 (99.25%), Query Frame = 2 
Query: 1349 ILDNFNCTNFHNNKESPEKNQQCQRKNSLPLNFTNYYYIPSAPASEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN 1747
            + DNFNCTNFHNNKESPEKNQQCQRKNSLPLNFTNYYYIPSAPASEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN
Sbjct:   13 LYDNFNCTNFHNNKESPEKNQQCQRKNSLPLNFTNYYYIPSAPASEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN 145
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000062192.1 (SMESG000062192.1)

HSP 1 Score: 221.09 bits (562), Expect = 9.862e-69
Identity = 114/133 (85.71%), Postives = 116/133 (87.22%), Query Frame = 2 
Query: 1349 ILDNFNCTNFHNNKESPEKNQQCQRKNSLPLNFTNYYYIPSAPASEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN 1747
            + DNFNCTNFHNNKESPEKNQQCQRKN+                SEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN
Sbjct:   13 LYDNFNCTNFHNNKESPEKNQQCQRKNT----------------SEELHENNFFNVDSSTAIELEHTDDLIYTVNSDIQIQSPPDIVRLSPLITSEVPLNMEPLTDQRNFQSSENYVNGDRTSVRPRNSLNDN 129
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000081807.1 (SMESG000081807.1)

HSP 1 Score: 87.8113 bits (216), Expect = 4.718e-18
Identity = 78/279 (27.96%), Postives = 116/279 (41.58%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAV--DVQNKNKSEINLYNNRIHRVDDLSK------------------FYNIIVCDIAKKVIWLNL-YLTWNQSK-----------LNSVENVFEKDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTP-TISAVPSDKRRRCYCCLYEKGRGFKQYCCVCQKPVFNEHSLK--TVVCLTVKL 1073
            VV+ L     N  RNV  DN+FTS++L + L S+ IT +GT+RKS   IP  FL     +F  + F   ++  +V+Y P  +R V+L+STMH+   V  D + +    +  YN     VD   +                  FY II        + LN+   TW   +           L    +   K +++     KS    +  K +    IL     PTP T+    + KR RC  C  ++ +  K  C  C + V  +H  K  T  CL   L
Sbjct:  190 VVKQLMLPLYNSGRNVTTDNFFTSYDLGQFLLSQNITLLGTMRKSRTEIPPEFLSKSREEFE-SKFAFTKDTALVSYAPPKNRTVVLLSTMHNRPVVFDDGRGRKPYMVLDYNMTKGAVDAFDQEVSFYSFAHKTRQWPMRLFYFIIDASCLNAFVLLNMNNPTWKDHEGSRRLDKRRLFLTKAAHSLMKPMIIARADNKS----ISHKPSVARAILAVGVKPTPSTLPGQVAKKRGRCQSCSRKQEQKVKHRCTKCSQFVCGKHGRKELTYTCLKCPL 463
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BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000070813.1 (SMESG000070813.1)

HSP 1 Score: 56.225 bits (134), Expect = 3.770e-8
Identity = 35/99 (35.35%), Postives = 53/99 (53.54%), Query Frame = 3 
Query:  384 NVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKSEINLYNNR 680
            +V  DN+FTS  LA  L +  I   GT+R +   IP  F   R  + + + FG+  N+T+V+Y+P   +AV L+ T  H   +  +  NK  I LY N+
Sbjct:  153 SVTTDNFFTSVPLALKLLNNKIYLTGTLRTNKREIPVQFQVSRRSELS-SNFGYNINLTLVSYIPKKGKAVNLLITKFHNNEICKEENNKPRIILYYNK 250
[Close]
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000067287.1 (SMESG000067287.1)

HSP 1 Score: 48.521 bits (114), Expect = 7.481e-6
Identity = 58/266 (21.80%), Postives = 104/266 (39.10%), Query Frame = 3 
Query:  342 VVEMLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNFLFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKNKS------EINLYNNRIHRVDDLS-------------KFYNIIVCDIAKKVIWLNLYL----TWNQSKLNSVENVFEKDLVVPNIQRKSK-LFLLKSKVAAIDEILINSSNPTPTISAVPSDKRR-----RCYCCLYEKGRGFKQYCC--------VCQKPVF 1028
            +V  L  ++ N   ++  DNY+ S  L+  L SK     GT+R +   +P      ++RK    V+   +N T V+   +  R V+ ++T +H + ++  N+ +       EI  YN  +  VD                ++Y  ++  +    +W + Y+    T +Q K         + L   + +R  + L   K      +EI  N +   P   + P + +R     RC  C  +  R    YCC        +C  P F
Sbjct:    7 LVMRLMQSYLNKGHHLFMDNYYNSLPLSNKLLSKKTHVTGTLRVNRKGLPQEITRRKLRK-GEHVWRR-QNQTYVSKWKD-KRDVLCLTTAYHPSMINTANRRQQEKRKPIEIVNYNQNMSGVDRADQMMSYYSCPRKTIRWYKKVIFHLLDVAVWNSFYIFKEKTGSQMKYIEYREAIIRSLTGMDNKRDGRTLVQFKRAQIQTNEINNNGTQHFPEKISPPENYQRKTYFQRCKQCYKQGIRKETSYCCKNCMSKPALCPAPCF 269
The following BLAST results are available for this feature:
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CABZ01072083.21.270e-1525.49dual specificity phosphatase 26 [Source:NCBI gene;... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1Y1MEP51.854e-3634.60DDE_Tnp_1_7 domain-containing protein (Fragment) O... [more]
A0A4C1VNV02.945e-3539.57PiggyBac transposable element-derived protein 4 OS... [more]
A0A4C1ZGA02.365e-3340.34PiggyBac transposable element-derived protein 4 OS... [more]
A0A4C1UEE45.298e-2941.97PiggyBac transposable element-derived protein 4 OS... [more]
A0A4C1U7C21.559e-2453.64PiggyBac transposable element-derived protein 4 OS... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSORLT00000027888.12.054e-1626.10pep primary_assembly:ASM223467v1:17:9217998:921921... [more]
ENSORLT00000037833.14.719e-1626.94piggyBac transposable element-derived protein 4-li... [more]
ENSORLT00000029842.11.412e-1226.67piggyBac transposable element-derived protein 4 [S... [more]
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BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062192.11.530e-8598.50SMESG000062192.1[more]
SMESG000062192.19.862e-6985.71SMESG000062192.1[more]
SMESG000081807.14.718e-1827.96SMESG000081807.1[more]
SMESG000070813.13.770e-835.35SMESG000070813.1[more]
SMESG000067287.17.481e-621.80SMESG000067287.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011417 ID=SMED30011417|Name=PiggyBac transposable element-derived protein 4|organism=Schmidtea mediterranea sexual|type=transcript|length=2082bp
GAGATCAGTGCAAACAAGGCCTGACCTGTCCCAGCAGACTTTGAAGGATA
AAAGTCTCGTAAAACTAGCACCTACGGATCCTCAAAATTAAATGAGGGTA
TCGAGTACACCGTCGGCCGCTTCCAAATGCTACTATGATAATACAATATA
AATGTTAGGTTACCTACCAGTGGATATAAAAATATCCACGGCCATCCAGA
ATAAAAATAAAATTAACATAGGAAAATATATTATTACAAAAACTACTTAC
AAGTTATCAGTTGGAAATATTTTGCAAATTTCAAAATTGGCATAAATTGA
AATTGTGATTATATCTGTCACTTATATAGTGATTTATATAGGTTGTCGAG
ATGCTTTGCGATACCTTTGAAAATATAAACAGGAACGTGATTTTCGATAA
CTATTTTACCAGTTTTGAGCTAGCACGGACATTGTTTTCCAAAGGAATAA
CATGTGTAGGCACTGTACGAAAAAGCCATATATCCATTCCAGGAAACTTT
TTATTTCATAGGATGAGGAAATTCGCAATAACTGTATTTGGCCATTGTCG
AAATATGACGATTGTTAACTATGTGCCGAACATTAGCAGGGCGGTTATAC
TTATCTCAACGATGCATCATACCACCGCAGTAGATGTCCAAAATAAAAAC
AAGTCCGAAATAAATTTGTACAATAACCGTATACATCGCGTAGATGACCT
CTCGAAATTTTATAATATTATTGTGTGTGATATTGCGAAAAAAGTTATTT
GGTTGAATTTATATCTTACCTGGAATCAGTCAAAGCTAAATTCGGTTGAA
AACGTTTTTGAAAAAGATTTAGTCGTTCCAAATATACAACGCAAAAGTAA
GTTATTTTTGTTGAAATCGAAAGTTGCTGCAATCGACGAGATATTAATAA
ACTCATCTAATCCAACCCCGACTATATCAGCAGTACCATCAGATAAGCGA
CGACGTTGTTACTGCTGCCTTTATGAAAAAGGACGAGGTTTTAAACAATA
CTGTTGTGTTTGTCAGAAACCAGTATTTAATGAGCATTCTCTGAAAACTG
TCGTTTGTCTAACTGTTAAACTTTTTATTTATACATGTTACACTCTGGCA
TGTATAAATAAATAGTTGAACAACATTGGCCACATTATTTGTACTACATT
TTTTTTCAATAAAACAAATGAATTCACTTTGTATTTTGTATTGGTGTCTC
TAAATCACAATGTGACAGTTTACGTTAGTCAGACAATAATAGGGTAAATG
TAAAATCTTTATTAAGTTCCATTTTTGTTGTTTATAATGCTTTTATTAAT
CTATTATTTATAATAAACTAGTATAAATTATTATTTATAGAAATTTTAAT
TTTAGATAATTTTAATTGTACAAACTTTCATAATAATAAAGAAAGTCCAG
AGAAAAATCAACAGTGTCAAAGGAAAAATAGTTTGCCATTAAATTTTACA
AATTATTATTATATTCCAAGTGCTCCAGCTTCTGAGGAATTGCATGAAAA
TAATTTTTTCAATGTTGATAGTTCAACAGCAATTGAGTTAGAGCACACTG
ACGATCTGATTTATACTGTAAATAGTGATATTCAAATACAATCTCCTCCT
GACATTGTAAGACTCTCTCCATTGATTACATCCGAAGTTCCACTTAACAT
GGAGCCATTAACAGATCAGAGAAACTTTCAATCATCTGAAAATTATGTGA
ATGGTGATAGAACTTCTGTTCGACCAAGGAATTCATTAAATGACAACTAA
TATTAATTATTCTTATGTTACATGTGCCAATTCTTATTTGTTCACTTATT
CTTATTATTCTAAAATGATTTGATTATTAATGGTTTTTATTTTTTGTAAT
TTTTAATGATCTAAGTTGTGATTTTAATTTATATTTATTTATAGTTATTC
ATCTGTATCTTTTGTCACTACAACATGAAAATGAGATAAATTTAATCGAA
ACTATCCAATTTCAAGATATTCTTAATTTTATTTTTCTTTATTGATCAGT
TTCTTTTTAAAATTTTTATTGGAGATTTACCAACTGTTTCTTTTTTGAAA
AATGTGTTCAATAAATTCGTCATTATTCTTTC
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protein sequence of SMED30011417-orf-1

>SMED30011417-orf-1 ID=SMED30011417-orf-1|Name=SMED30011417-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=255bp
MLCDTFENINRNVIFDNYFTSFELARTLFSKGITCVGTVRKSHISIPGNF
LFHRMRKFAITVFGHCRNMTIVNYVPNISRAVILISTMHHTTAVDVQNKN
KSEINLYNNRIHRVDDLSKFYNIIVCDIAKKVIWLNLYLTWNQSKLNSVE
NVFEKDLVVPNIQRKSKLFLLKSKVAAIDEILINSSNPTPTISAVPSDKR
RRCYCCLYEKGRGFKQYCCVCQKPVFNEHSLKTVVCLTVKLFIYTCYTLA
CINK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR029526PGBD
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029526PiggyBac transposable element-derived proteinPFAMPF13843DDE_Tnp_1_7coord: 2..120
e-value: 1.1E-13
score: 51.3