helix-loop-helix protein 1

Overview
Namehelix-loop-helix protein 1
Smed IDSMED30011397
Length (bp)431
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of helix-loop-helix protein 1 (SMED30011397) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for helix-loop-helix protein 1 (SMED30011397) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011397

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011397 dd_Smed_v6_31217_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
central nervous systemSMED30011397 KF487113smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30011397 KF487113smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30011397 KF487113smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30011397 KF487113smed_ncbi_20240424PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Match: NHLH1 (nescient helix-loop-helix 1 [Source:HGNC Symbol;Acc:HGNC:7817])

HSP 1 Score: 95.5153 bits (236), Expect = 1.566e-25
Identity = 45/60 (75.00%), Postives = 51/60 (85.00%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +T KYR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   72 ATAKYRTAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 131
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Match: NHLH2 (nescient helix-loop-helix 2 [Source:HGNC Symbol;Acc:HGNC:7818])

HSP 1 Score: 87.8113 bits (216), Expect = 1.638e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Match: NHLH2 (nescient helix-loop-helix 2 [Source:HGNC Symbol;Acc:HGNC:7818])

HSP 1 Score: 87.8113 bits (216), Expect = 1.638e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Match: TAL2 (TAL bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:11557])

HSP 1 Score: 63.1586 bits (152), Expect = 2.933e-13
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
             RER R +  NSAF +LR L+PT PPDKK+SK E LRLA+RY+++L  V+  Q
Sbjct:    9 TRERWRQQNVNSAFAKLRKLIPTHPPDKKLSKNETLRLAMRYINFLVKVLGEQ 61
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Match: SCX (scleraxis bHLH transcription factor [Source:HGNC Symbol;Acc:HGNC:32322])

HSP 1 Score: 61.6178 bits (148), Expect = 6.347e-12
Identity = 29/55 (52.73%), Postives = 39/55 (70.91%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            R+   ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   77 RHTANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVL 131
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Match: hlh-15 (Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18590])

HSP 1 Score: 93.5893 bits (231), Expect = 9.315e-26
Identity = 51/73 (69.86%), Postives = 61/73 (83.56%), Query Frame = 3 
Query:  156 ESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLD 374
            E + KLSK ERR+RRR+T KYRN HA RERIRVE+FN AF QLR+LLPT P +KK+SKIEILR +I Y+S+LD
Sbjct:   13 EEYRKLSKAERRKRRRATPKYRNLHATRERIRVESFNMAFSQLRALLPTLPVEKKLSKIEILRFSIAYISFLD 85
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Match: hlh-13 (Helix-loop-helix protein 13 [Source:UniProtKB/Swiss-Prot;Acc:Q20561])

HSP 1 Score: 58.151 bits (139), Expect = 2.269e-11
Identity = 28/57 (49.12%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            R   + RER R+ + N AFI+LR+ +PTFP +K++SKI+ L LAI Y++ LD V+ T
Sbjct:   43 RQTASIRERKRMCSINVAFIELRNYIPTFPYEKRLSKIDTLNLAIAYINMLDDVLRT 99
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Match: ngn-1 (NeuroGeNin [Source:UniProtKB/TrEMBL;Acc:Q95XG7])

HSP 1 Score: 50.8322 bits (120), Expect = 1.921e-8
Identity = 26/62 (41.94%), Postives = 36/62 (58.06%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ*PPS 404
            R+   ARER R+ + N A   LR +LP  P + KM+KIE LR A  Y++ L F ++   P S
Sbjct:   64 RDKANARERRRMNSLNDALEHLRGILPALPDEPKMTKIETLRKAQEYIASLSFQLSGGSPDS 125
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Match: hlh-8 (Twist-related protein [Source:UniProtKB/Swiss-Prot;Acc:Q11094])

HSP 1 Score: 49.6766 bits (117), Expect = 5.222e-8
Identity = 25/46 (54.35%), Postives = 31/46 (67.39%), Query Frame = 3 
Query:  237 RERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLD 374
            RER R +  N AF  LR L+P+ P D KMSKI  LR+A  Y+S+LD
Sbjct:   28 RERQRTKELNDAFTLLRKLIPSMPSD-KMSKIHTLRIATDYISFLD 72
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Match: hlh-6 (Helix-loop-helix protein 6 [Source:UniProtKB/Swiss-Prot;Acc:Q10007])

HSP 1 Score: 46.595 bits (109), Expect = 1.088e-6
Identity = 22/49 (44.90%), Postives = 33/49 (67.35%), Query Frame = 3 
Query:  237 RERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            RER RV   N  + +LR  LP    +K++SK++ LRLAIRY+ +LD ++
Sbjct:  181 RERCRVRNVNDGYERLRKHLPVHFDEKRISKVDTLRLAIRYIKHLDNLL 229
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Match: HLH4C (gene:FBgn0011277 transcript:FBtr0070691)

HSP 1 Score: 92.8189 bits (229), Expect = 2.055e-24
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            +T KYR AHA RERIRVEAFN +F +LR LLPT PPDKK+SKIEIL+LAI Y++YL+ V+ T
Sbjct:  105 ATLKYRTAHATRERIRVEAFNVSFAELRKLLPTLPPDKKLSKIEILKLAICYIAYLNHVLET 166
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Match: HLH4C (gene:FBgn0011277 transcript:FBtr0331163)

HSP 1 Score: 92.8189 bits (229), Expect = 3.622e-24
Identity = 43/62 (69.35%), Postives = 52/62 (83.87%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            +T KYR AHA RERIRVEAFN +F +LR LLPT PPDKK+SKIEIL+LAI Y++YL+ V+ T
Sbjct:  105 ATLKYRTAHATRERIRVEAFNVSFAELRKLLPTLPPDKKLSKIEILKLAICYIAYLNHVLET 166
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Match: HLH3B (gene:FBgn0011276 transcript:FBtr0070506)

HSP 1 Score: 58.151 bits (139), Expect = 1.629e-10
Identity = 26/49 (53.06%), Postives = 35/49 (71.43%), Query Frame = 3 
Query:  237 RERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            RER R +  + AF +LR L+PT PPDKK+SK EILR AI+Y+  L  ++
Sbjct:  173 RERWRQQNVSGAFAELRKLVPTHPPDKKLSKNEILRSAIKYIKLLTGIL 221
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Match: HLH54F (gene:FBgn0022740 transcript:FBtr0089547)

HSP 1 Score: 56.6102 bits (135), Expect = 3.878e-10
Identity = 26/57 (45.61%), Postives = 38/57 (66.67%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            RNA  ARER+R+   +SA+ +L++ LP  PPD K+SK++ LRLA  Y+  L   + T
Sbjct:   33 RNAANARERMRMRVLSSAYGRLKTKLPNIPPDTKLSKLDTLRLATLYIKQLITAVET 89
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Match: Fer2 (gene:FBgn0038402 transcript:FBtr0301709)

HSP 1 Score: 55.4546 bits (132), Expect = 1.237e-9
Identity = 31/62 (50.00%), Postives = 42/62 (67.74%), Query Frame = 3 
Query:  219 RNAHAARERIRVEA-FNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ*PP 401
            R A   RER R+++  NSAF +LR  +PTFP +K++SKI+ LRLAI Y+S L  V+ T   P
Sbjct:  149 RQAANVRERKRIQSSINSAFDELRVHVPTFPYEKRLSKIDTLRLAIAYISLLREVLQTDYDP 210
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Match: nhlh2 (nescient helix loop helix 2 [Source:ZFIN;Acc:ZDB-GENE-040426-1809])

HSP 1 Score: 92.0485 bits (227), Expect = 1.773e-24
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = 3 
Query:  216 YRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            YR+AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   65 YRSAHATRERIRVEAFNVAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 120
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Match: tcf15 (transcription factor 15 [Source:ZFIN;Acc:ZDB-GENE-980605-20])

HSP 1 Score: 66.6254 bits (161), Expect = 3.668e-14
Identity = 32/57 (56.14%), Postives = 42/57 (73.68%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            K RNA  ARER R ++ N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   64 KQRNAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVL 120
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Match: scxa (scleraxis bHLH transcription factor a [Source:ZFIN;Acc:ZDB-GENE-060503-414])

HSP 1 Score: 66.6254 bits (161), Expect = 4.804e-14
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + RNA  ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   79 RQRNAANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVL 135
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Match: CR626923.2 (scleraxis bHLH transcription factor b [Source:NCBI gene;Acc:567817])

HSP 1 Score: 64.3142 bits (155), Expect = 3.059e-13
Identity = 30/57 (52.63%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + RN   ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   77 RQRNTANARERERTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Match: si:ch211-246m6.4 (si:ch211-246m6.4 [Source:ZFIN;Acc:ZDB-GENE-050208-498])

HSP 1 Score: 64.3142 bits (155), Expect = 3.278e-13
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            K R A  ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   83 KQRQAANARERDRTHSVNTAFTSLRTLIPTEPADRKLSKIETLRLASSYISHLANVL 139
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Match: clybl (citrate lyase beta like [Source:Xenbase;Acc:XB-GENE-989411])

HSP 1 Score: 97.0561 bits (240), Expect = 2.681e-26
Identity = 45/60 (75.00%), Postives = 51/60 (85.00%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +T KYR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   67 ATAKYRTAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 126
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Match: TAL2 (TAL bHLH transcription factor 2 [Source:HGNC Symbol;Acc:HGNC:11557])

HSP 1 Score: 62.7734 bits (151), Expect = 3.074e-13
Identity = 29/53 (54.72%), Postives = 39/53 (73.58%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
             RER R +  NSAF +LR L+PT PPDKK+SK E LRLA+RY+++L  ++  Q
Sbjct:    9 TRERWRQQNVNSAFAELRKLIPTHPPDKKLSKNETLRLAMRYINFLIDILGDQ 61
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Match: tcf15 (transcription factor 15 [Source:NCBI gene;Acc:549260])

HSP 1 Score: 63.1586 bits (152), Expect = 8.060e-13
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            K R A  ARER R ++ N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   62 KQRQAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVL 118
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Match: adra1d (adrenoceptor alpha 1D [Source:Xenbase;Acc:XB-GENE-6050827])

HSP 1 Score: 63.929 bits (154), Expect = 9.966e-13
Identity = 30/60 (50.00%), Postives = 40/60 (66.67%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
               K R A  ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  ++
Sbjct:   99 GVSKQRQAANARERDRTHSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLANIL 158
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Match: hnrnpc (heterogeneous nuclear ribonucleoprotein C (C1/C2) [Source:Xenbase;Acc:XB-GENE-489141])

HSP 1 Score: 62.7734 bits (151), Expect = 1.863e-12
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            K R A  ARER R ++ N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   62 KQRQAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVL 118
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Match: Nhlh1 (nescient helix loop helix 1 [Source:MGI Symbol;Acc:MGI:98481])

HSP 1 Score: 95.9005 bits (237), Expect = 6.819e-26
Identity = 45/60 (75.00%), Postives = 51/60 (85.00%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +T KYR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   72 ATAKYRTAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 131
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Match: Nhlh2 (nescient helix loop helix 2 [Source:MGI Symbol;Acc:MGI:97324])

HSP 1 Score: 87.8113 bits (216), Expect = 1.240e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Match: Nhlh2 (nescient helix loop helix 2 [Source:MGI Symbol;Acc:MGI:97324])

HSP 1 Score: 87.8113 bits (216), Expect = 1.240e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Match: Nhlh2 (nescient helix loop helix 2 [Source:MGI Symbol;Acc:MGI:97324])

HSP 1 Score: 87.8113 bits (216), Expect = 1.240e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Match: Tal2 (T cell acute lymphocytic leukemia 2 [Source:MGI Symbol;Acc:MGI:99540])

HSP 1 Score: 62.7734 bits (151), Expect = 2.993e-13
Identity = 29/53 (54.72%), Postives = 40/53 (75.47%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
             RER R ++ N+AF +LR L+PT PPDKK+SK E LRLA+RY+++L  V+  Q
Sbjct:    9 TRERWRQQSVNNAFAKLRKLIPTHPPDKKLSKNETLRLAMRYINFLVKVLGEQ 61
[Close]
BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Match: sp|Q02576|HEN1_MOUSE (Helix-loop-helix protein 1 OS=Mus musculus OX=10090 GN=Nhlh1 PE=2 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 4.770e-25
Identity = 45/60 (75.00%), Postives = 51/60 (85.00%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +T KYR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   72 ATAKYRTAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 131
[Close]
BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Match: sp|Q02575|HEN1_HUMAN (Helix-loop-helix protein 1 OS=Homo sapiens OX=9606 GN=NHLH1 PE=1 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 7.523e-25
Identity = 45/60 (75.00%), Postives = 51/60 (85.00%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +T KYR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   72 ATAKYRTAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 131
[Close]
BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Match: sp|Q02577|HEN2_HUMAN (Helix-loop-helix protein 2 OS=Homo sapiens OX=9606 GN=NHLH2 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 7.868e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Match: sp|Q64221|HEN2_MOUSE (Helix-loop-helix protein 2 OS=Mus musculus OX=10090 GN=Nhlh2 PE=1 SV=2)

HSP 1 Score: 87.8113 bits (216), Expect = 8.672e-22
Identity = 41/54 (75.93%), Postives = 47/54 (87.04%), Query Frame = 3 
Query:  222 NAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   80 SAHATRERIRVEAFNLAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 133
[Close]
BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Match: sp|Q16559|TAL2_HUMAN (T-cell acute lymphocytic leukemia protein 2 OS=Homo sapiens OX=9606 GN=TAL2 PE=1 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.409e-12
Identity = 30/53 (56.60%), Postives = 39/53 (73.58%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
             RER R +  NSAF +LR L+PT PPDKK+SK E LRLA+RY+++L  V+  Q
Sbjct:    9 TRERWRQQNVNSAFAKLRKLIPTHPPDKKLSKNETLRLAMRYINFLVKVLGEQ 61
[Close]
BLAST of helix-loop-helix protein 1 vs. TrEMBL
Match: U5YQE1 (Hlh1 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=hlh1 PE=2 SV=1)

HSP 1 Score: 149.058 bits (375), Expect = 3.083e-44
Identity = 80/80 (100.00%), Postives = 80/80 (100.00%), Query Frame = 3 
Query:  153 DESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
            DESWSKLSKEERRRRRRSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ
Sbjct:    1 DESWSKLSKEERRRRRRSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 80
[Close]
BLAST of helix-loop-helix protein 1 vs. TrEMBL
Match: B7QIA4 (Helix-loop-helix protein, putative OS=Ixodes scapularis OX=6945 GN=8041671 PE=4 SV=1)

HSP 1 Score: 104.375 bits (259), Expect = 3.211e-26
Identity = 57/79 (72.15%), Postives = 66/79 (83.54%), Query Frame = 3 
Query:  147 DQDESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            D  ++   LS+E+RRRRRR+TQKYR AHA RERIRVEAFN AF QLR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   23 DGHQAVVSLSREDRRRRRRATQKYRLAHATRERIRVEAFNVAFAQLRRLLPTLPPDKKLSKIEILRLAICYISYLNHVL 101
[Close]
BLAST of helix-loop-helix protein 1 vs. TrEMBL
Match: A0A5B7IX19 (Helix-loop-helix protein 1 OS=Portunus trituberculatus OX=210409 GN=Nhlh1 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 4.675e-25
Identity = 55/78 (70.51%), Postives = 65/78 (83.33%), Query Frame = 3 
Query:  150 QDESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + ES   ++KEERRRRRR+T KYR AHA RER+RVEAFN AF QLR LLPT PPDKK+SKIEILRLAI Y++YL+ V+
Sbjct:    7 ERESVLAMTKEERRRRRRATLKYRTAHATRERMRVEAFNVAFAQLRKLLPTLPPDKKLSKIEILRLAICYIAYLNHVL 84
[Close]
BLAST of helix-loop-helix protein 1 vs. TrEMBL
Match: A0A1S3CU76 (helix-loop-helix protein 1 OS=Diaphorina citri OX=121845 GN=LOC103505341 PE=4 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 4.877e-25
Identity = 58/79 (73.42%), Postives = 67/79 (84.81%), Query Frame = 3 
Query:  153 DESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            D S   LS+EERRRRRR+TQKYR AHA RER+RVEAFNSAF +LR LLPT PPDKK+SKIEILRLAI Y++YL+ V+ T
Sbjct:   31 DGSTQALSREERRRRRRATQKYRTAHATRERVRVEAFNSAFGELRKLLPTLPPDKKLSKIEILRLAICYIAYLNNVLET 109
[Close]
BLAST of helix-loop-helix protein 1 vs. TrEMBL
Match: A0A3Q4GLC4 (BHLH domain-containing protein OS=Neolamprologus brichardi OX=32507 PE=4 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.087e-24
Identity = 54/78 (69.23%), Postives = 63/78 (80.77%), Query Frame = 3 
Query:  150 QDESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            QD      SKEE+RR+RR+T KYR+AHA RER+RV AFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYLD V+
Sbjct:   36 QDGETKARSKEEKRRKRRATAKYRSAHATRERVRVVAFNVAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLDHVL 113
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Match: ENSAMXT00000026732.2 (helix-loop-helix protein 2-like [Source:NCBI gene;Acc:103035065])

HSP 1 Score: 91.2781 bits (225), Expect = 2.422e-24
Identity = 43/56 (76.79%), Postives = 49/56 (87.50%), Query Frame = 3 
Query:  216 YRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            YR+AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   65 YRSAHATRERIRVEAFNVAFGELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 120
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Match: nhlh2 (helix-loop-helix protein 2-like [Source:NCBI gene;Acc:103037174])

HSP 1 Score: 89.3521 bits (220), Expect = 1.246e-23
Identity = 43/56 (76.79%), Postives = 48/56 (85.71%), Query Frame = 3 
Query:  216 YRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            YR AHA RERIRVEAFN AF +LR LLPT PPDKK+SKIEILRLAI Y+SYL+ V+
Sbjct:   65 YRLAHATRERIRVEAFNVAFAELRKLLPTLPPDKKLSKIEILRLAICYISYLNHVL 120
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Match: tcf15 (transcription factor 15 [Source:NCBI gene;Acc:103040145])

HSP 1 Score: 67.781 bits (164), Expect = 1.417e-14
Identity = 33/59 (55.93%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            K RNA  ARER R ++ N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+ T
Sbjct:   61 KQRNAANARERDRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVLLT 119
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Match: scxa (scleraxis bHLH transcription factor a [Source:ZFIN;Acc:ZDB-GENE-060503-414])

HSP 1 Score: 67.0106 bits (162), Expect = 3.659e-14
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + RNA  ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   78 RQRNAANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVL 134
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Match: scxb (basic helix-loop-helix transcription factor scleraxis-like [Source:NCBI gene;Acc:103029811])

HSP 1 Score: 64.6994 bits (156), Expect = 2.423e-13
Identity = 31/57 (54.39%), Postives = 41/57 (71.93%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + RNA  ARER R  + N AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L+ V+
Sbjct:   78 RQRNAANARERDRTNSVNVAFTALRTLIPTEPADRKLSKIETLRLASSYISHLENVL 134
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002470.1 (pep scaffold:Pmarinus_7.0:GL487131:18401:21594:-1 gene:ENSPMAG00000002259.1 transcript:ENSPMAT00000002470.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.3066 bits (142), Expect = 9.403e-13
Identity = 29/53 (54.72%), Postives = 39/53 (73.58%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
            +RER R +  N AF +LR LLPT PPD+K+SK EILRLA+RY+ +L  ++  Q
Sbjct:   29 SRERWRQQNVNGAFSELRRLLPTHPPDRKLSKNEILRLAMRYIDFLVALLRDQ 81
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Match: scxa (scleraxis bHLH transcription factor a [Source:ZFIN;Acc:ZDB-GENE-060503-414])

HSP 1 Score: 60.077 bits (144), Expect = 1.084e-12
Identity = 34/79 (43.04%), Postives = 47/79 (59.49%), Query Frame = 3 
Query:  135 AHFPDQDESWSKLSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYL 371
             + P QD+    LS +E  R        R A  ARER R ++ NSAF  LR+L+PT P D+K+SK+E L LA  Y+++L
Sbjct:   14 CNNPSQDD----LSCKESGRLMVPCGPQRRAANARERDRTQSVNSAFTALRTLIPTEPADRKLSKLETLLLASSYIAHL 88
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004072.1 (pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 gene:ENSPMAG00000003727.1 transcript:ENSPMAT00000004072.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 56.6102 bits (135), Expect = 9.664e-11
Identity = 26/60 (43.33%), Postives = 43/60 (71.67%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ*P 398
            R A   RER R+++ N+AF  LR+ +PT P +K++SK++ LRLAI Y+S+L  ++ ++ P
Sbjct:  133 RQAANQRERRRMQSINAAFEGLRAHIPTLPYEKRLSKVDTLRLAIGYISFLGELVHSERP 192
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Match: si:dkey-34f9.3 (si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-410])

HSP 1 Score: 53.5286 bits (127), Expect = 2.485e-10
Identity = 27/51 (52.94%), Postives = 37/51 (72.55%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYL 371
            R A   RER R+ + NSAF +LR  +PTFP +K++SKI+ LRLAI Y++ L
Sbjct:   35 RTAANVRERRRMLSINSAFEELRCHVPTFPYEKRLSKIDTLRLAIAYIALL 85
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Match: TWIST1 (twist family bHLH transcription factor 1 [Source:HGNC Symbol;Acc:HGNC:12428])

HSP 1 Score: 53.9138 bits (128), Expect = 4.692e-10
Identity = 31/72 (43.06%), Postives = 42/72 (58.33%), Query Frame = 3 
Query:  171 LSKEEXXXXXXSTQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIA 386
            LS EE        Q+ R     RER R ++ N AF  LR ++PT P D K+SKI+ L+LA RY+ +L  V+A
Sbjct:   88 LSGEE-------VQQQRFVANVRERQRTQSLNEAFASLRQIIPTLPSD-KLSKIQTLKLATRYIDFLYQVLA 151
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Match: EDO46194 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN4])

HSP 1 Score: 64.6994 bits (156), Expect = 3.224e-13
Identity = 28/55 (50.91%), Postives = 41/55 (74.55%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            R     +ER R ++ N AF ++R LLPT+PPDKK+SK+EILR A++Y+ +LD V+
Sbjct:   52 RTVTNKKERWRQQSVNLAFAEIRKLLPTYPPDKKLSKVEILRTAVKYIQFLDGVL 106
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Match: EDO42924 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RZZ0])

HSP 1 Score: 58.9214 bits (141), Expect = 9.142e-13
Identity = 29/57 (50.88%), Postives = 39/57 (68.42%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            K R A  ARER R  + N+AF  LR L+PT P D+K+SKIE LRLA  Y+++L  ++
Sbjct:    1 KQRQAANARERNRTHSVNAAFNALRLLIPTEPSDRKLSKIETLRLASSYIAHLSTIL 57
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Match: EDO46193 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN3])

HSP 1 Score: 56.6102 bits (135), Expect = 7.618e-12
Identity = 24/51 (47.06%), Postives = 40/51 (78.43%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIA 386
            +RER R +  N AF +LR L+PT+PP++K+SK EILR A++Y+ +L+ +++
Sbjct:    8 SRERWRQQHVNLAFAELRKLIPTYPPERKLSKNEILRFAMKYIKFLENILS 58
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Match: EDO46140 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQN5])

HSP 1 Score: 58.5362 bits (140), Expect = 1.712e-11
Identity = 27/50 (54.00%), Postives = 37/50 (74.00%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            +RER R    N AF +LR LLPT+PPDKK+SK EILR  ++Y+ +LD ++
Sbjct:   73 SRERWRQYQVNLAFAELRKLLPTYPPDKKLSKHEILRSTMKYIKFLDGLL 122
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Match: NVAS-C2 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T1A5])

HSP 1 Score: 55.0694 bits (131), Expect = 2.746e-11
Identity = 25/52 (48.08%), Postives = 35/52 (67.31%), Query Frame = 3 
Query:  237 RERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
            RERIRV   N  + +LR  LP  P DK+MSK+E LR AIRY+ +L+ ++  +
Sbjct:   17 RERIRVRHVNEGYARLREHLPEEPSDKRMSKVETLRAAIRYIKHLESLLQAE 68
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Match: tcf15 (transcription factor 15 [Source:NCBI gene;Acc:101172734])

HSP 1 Score: 66.2402 bits (160), Expect = 4.882e-14
Identity = 32/59 (54.24%), Postives = 43/59 (72.88%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIAT 389
            K R+A  ARER R ++ N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+ T
Sbjct:   62 KQRSAANARERGRTQSVNTAFTALRTLIPTEPVDRKLSKIETLRLASSYISHLANVLLT 120
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Match: ENSORLT00000017495.2 (pep primary_assembly:ASM223467v1:4:24388791:24389132:-1 gene:ENSORLG00000013953.2 transcript:ENSORLT00000017495.2 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 64.6994 bits (156), Expect = 5.165e-14
Identity = 30/55 (54.55%), Postives = 42/55 (76.36%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ*P 398
            +RER R +  N AF +LR L+PT PPD+K+SK EILRLA+RY+ +LD +++ Q P
Sbjct:    9 SRERWRQQNVNGAFAELRRLIPTHPPDRKLSKNEILRLALRYIRFLDQLLSEQDP 63
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Match: scxb (scleraxis bHLH transcription factor [Source:NCBI gene;Acc:101167982])

HSP 1 Score: 63.5438 bits (153), Expect = 9.844e-13
Identity = 30/57 (52.63%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  213 KYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVI 383
            + R A  ARER R  + N+AF  LR+L+PT P D+K+SKIE LRLA  Y+S+L  V+
Sbjct:   96 RQRTAANARERDRTNSVNTAFTALRTLIPTEPADRKLSKIETLRLASSYISHLGNVL 152
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Match: tal1 (T-cell acute lymphocytic leukemia protein 1 [Source:NCBI gene;Acc:101174459])

HSP 1 Score: 64.3142 bits (155), Expect = 1.341e-12
Identity = 29/53 (54.72%), Postives = 40/53 (75.47%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
            +RER R +  N AF +LR L+PT PPDKK+SK EILRLA++Y+S+L  ++  Q
Sbjct:  232 SRERWRQQNVNGAFAELRKLIPTHPPDKKLSKNEILRLAMKYISFLSNLLEDQ 284
[Close]
BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Match: tal2 (TAL bHLH transcription factor 2 [Source:NCBI gene;Acc:101160485])

HSP 1 Score: 60.8474 bits (146), Expect = 1.545e-12
Identity = 28/53 (52.83%), Postives = 40/53 (75.47%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
             RER R    N+AF +LR L+PT PP+KK+SK EILRLA+RY+++L  ++ +Q
Sbjct:    9 TRERWRQHNVNTAFAELRKLIPTHPPEKKLSKNEILRLAMRYINFLVRLLESQ 61
[Close]
BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Match: SMESG000018258.1 (SMESG000018258.1)

HSP 1 Score: 58.5362 bits (140), Expect = 3.207e-11
Identity = 33/76 (43.42%), Postives = 45/76 (59.21%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYL------DFVIATQ*PPSEIY 413
            ST   R+A  +RER R+   +SAFI L+S LP  P D K+SK++ L+LA  Y++YL      DF   T  PP  +Y
Sbjct:   36 STLAQRSAANSRERSRMRVLSSAFIDLKSALPWVPKDTKLSKLDTLKLAAGYIAYLKSVLVEDFSGTTLPPPWSVY 111
[Close]
BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Match: SMESG000067729.1 (SMESG000067729.1)

HSP 1 Score: 58.9214 bits (141), Expect = 9.088e-11
Identity = 30/64 (46.88%), Postives = 44/64 (68.75%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ*PPSEI 410
            R ++ +RER R ++ N+AF  LR +LP  PPDKK+SK EILR AI+Y+  L+ ++  Q   +EI
Sbjct:   53 RKSYNSRERERQQSVNTAFNDLRQILPCHPPDKKLSKHEILRQAIKYIRILEEILNFQSNHNEI 116
[Close]
BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Match: SMESG000045286.1 (SMESG000045286.1)

HSP 1 Score: 55.4546 bits (132), Expect = 1.184e-9
Identity = 25/46 (54.35%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  234 ARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYL 371
             RER R+ + N+AF +LRS +PTFP ++++SKI+ LRLAI Y+S L
Sbjct:   57 VRERNRIMSINTAFAELRSRVPTFPYERRLSKIDTLRLAIAYISLL 102
[Close]
BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Match: SMESG000079648.1 (SMESG000079648.1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.367e-9
Identity = 25/58 (43.10%), Postives = 41/58 (70.69%), Query Frame = 3 
Query:  219 RNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYLDFVIATQ 392
            R A   RER R+++ N AF  LR+ +PT P +K++SK++ LRLAI Y+++L  ++ T+
Sbjct:  104 RQAANLRERRRMQSINEAFEGLRAHIPTLPYEKRLSKVDTLRLAIGYINFLQDLVKTE 161
[Close]
BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Match: SMESG000019274.1 (SMESG000019274.1)

HSP 1 Score: 53.5286 bits (127), Expect = 1.996e-9
Identity = 27/56 (48.21%), Postives = 40/56 (71.43%), Query Frame = 3 
Query:  204 STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAIRYMSYL 371
            +T + R+A   RER R+   N AF +LR L+PTF  +K++S+IE LRLAI Y+S++
Sbjct:  112 TTIEQRHAANIRERRRMNNLNKAFDRLRQLVPTFAYEKQLSRIETLRLAITYISFM 167
The following BLAST results are available for this feature:
BLAST of helix-loop-helix protein 1 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NHLH11.566e-2575.00nescient helix-loop-helix 1 [Source:HGNC Symbol;Ac... [more]
NHLH21.638e-2275.93nescient helix-loop-helix 2 [Source:HGNC Symbol;Ac... [more]
NHLH21.638e-2275.93nescient helix-loop-helix 2 [Source:HGNC Symbol;Ac... [more]
TAL22.933e-1356.60TAL bHLH transcription factor 2 [Source:HGNC Symbo... [more]
SCX6.347e-1252.73scleraxis bHLH transcription factor [Source:HGNC S... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
hlh-159.315e-2669.86Helix Loop Helix [Source:UniProtKB/TrEMBL;Acc:Q18... [more]
hlh-132.269e-1149.12Helix-loop-helix protein 13 [Source:UniProtKB/Swi... [more]
ngn-11.921e-841.94NeuroGeNin [Source:UniProtKB/TrEMBL;Acc:Q95XG7][more]
hlh-85.222e-854.35Twist-related protein [Source:UniProtKB/Swiss-Pro... [more]
hlh-61.088e-644.90Helix-loop-helix protein 6 [Source:UniProtKB/Swis... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
HLH4C2.055e-2469.35gene:FBgn0011277 transcript:FBtr0070691[more]
HLH4C3.622e-2469.35gene:FBgn0011277 transcript:FBtr0331163[more]
HLH3B1.629e-1053.06gene:FBgn0011276 transcript:FBtr0070506[more]
HLH54F3.878e-1045.61gene:FBgn0022740 transcript:FBtr0089547[more]
Fer21.237e-950.00gene:FBgn0038402 transcript:FBtr0301709[more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nhlh21.773e-2476.79nescient helix loop helix 2 [Source:ZFIN;Acc:ZDB-G... [more]
tcf153.668e-1456.14transcription factor 15 [Source:ZFIN;Acc:ZDB-GENE-... [more]
scxa4.804e-1454.39scleraxis bHLH transcription factor a [Source:ZFIN... [more]
CR626923.23.059e-1352.63scleraxis bHLH transcription factor b [Source:NCBI... [more]
si:ch211-246m6.43.278e-1354.39si:ch211-246m6.4 [Source:ZFIN;Acc:ZDB-GENE-050208-... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
clybl2.681e-2675.00citrate lyase beta like [Source:Xenbase;Acc:XB-GEN... [more]
TAL23.074e-1354.72TAL bHLH transcription factor 2 [Source:HGNC Symbo... [more]
tcf158.060e-1354.39transcription factor 15 [Source:NCBI gene;Acc:5492... [more]
adra1d9.966e-1350.00adrenoceptor alpha 1D [Source:Xenbase;Acc:XB-GENE-... [more]
hnrnpc1.863e-1254.39heterogeneous nuclear ribonucleoprotein C (C1/C2) ... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nhlh16.819e-2675.00nescient helix loop helix 1 [Source:MGI Symbol;Acc... [more]
Nhlh21.240e-2275.93nescient helix loop helix 2 [Source:MGI Symbol;Acc... [more]
Nhlh21.240e-2275.93nescient helix loop helix 2 [Source:MGI Symbol;Acc... [more]
Nhlh21.240e-2275.93nescient helix loop helix 2 [Source:MGI Symbol;Acc... [more]
Tal22.993e-1354.72T cell acute lymphocytic leukemia 2 [Source:MGI Sy... [more]
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BLAST of helix-loop-helix protein 1 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q02576|HEN1_MOUSE4.770e-2575.00Helix-loop-helix protein 1 OS=Mus musculus OX=1009... [more]
sp|Q02575|HEN1_HUMAN7.523e-2575.00Helix-loop-helix protein 1 OS=Homo sapiens OX=9606... [more]
sp|Q02577|HEN2_HUMAN7.868e-2275.93Helix-loop-helix protein 2 OS=Homo sapiens OX=9606... [more]
sp|Q64221|HEN2_MOUSE8.672e-2275.93Helix-loop-helix protein 2 OS=Mus musculus OX=1009... [more]
sp|Q16559|TAL2_HUMAN1.409e-1256.60T-cell acute lymphocytic leukemia protein 2 OS=Hom... [more]
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BLAST of helix-loop-helix protein 1 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U5YQE13.083e-44100.00Hlh1 protein (Fragment) OS=Schmidtea mediterranea ... [more]
B7QIA43.211e-2672.15Helix-loop-helix protein, putative OS=Ixodes scapu... [more]
A0A5B7IX194.675e-2570.51Helix-loop-helix protein 1 OS=Portunus tritubercul... [more]
A0A1S3CU764.877e-2573.42helix-loop-helix protein 1 OS=Diaphorina citri OX=... [more]
A0A3Q4GLC41.087e-2469.23BHLH domain-containing protein OS=Neolamprologus b... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000026732.22.422e-2476.79helix-loop-helix protein 2-like [Source:NCBI gene;... [more]
nhlh21.246e-2376.79helix-loop-helix protein 2-like [Source:NCBI gene;... [more]
tcf151.417e-1455.93transcription factor 15 [Source:NCBI gene;Acc:1030... [more]
scxa3.659e-1454.39scleraxis bHLH transcription factor a [Source:ZFIN... [more]
scxb2.423e-1354.39basic helix-loop-helix transcription factor sclera... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000002470.19.403e-1354.72pep scaffold:Pmarinus_7.0:GL487131:18401:21594:-1 ... [more]
scxa1.084e-1243.04scleraxis bHLH transcription factor a [Source:ZFIN... [more]
ENSPMAT00000004072.19.664e-1143.33pep scaffold:Pmarinus_7.0:GL492732:1750:4325:-1 ge... [more]
si:dkey-34f9.32.485e-1052.94si:dkey-34f9.3 [Source:ZFIN;Acc:ZDB-GENE-081104-41... [more]
TWIST14.692e-1043.06twist family bHLH transcription factor 1 [Source:H... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of helix-loop-helix protein 1 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO461943.224e-1350.91Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO429249.142e-1350.88Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO461937.618e-1247.06Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO461401.712e-1154.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
NVAS-C22.746e-1148.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of helix-loop-helix protein 1 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
tcf154.882e-1454.24transcription factor 15 [Source:NCBI gene;Acc:1011... [more]
ENSORLT00000017495.25.165e-1454.55pep primary_assembly:ASM223467v1:4:24388791:243891... [more]
scxb9.844e-1352.63scleraxis bHLH transcription factor [Source:NCBI g... [more]
tal11.341e-1254.72T-cell acute lymphocytic leukemia protein 1 [Sourc... [more]
tal21.545e-1252.83TAL bHLH transcription factor 2 [Source:NCBI gene;... [more]
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BLAST of helix-loop-helix protein 1 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000018258.13.207e-1143.42SMESG000018258.1[more]
SMESG000067729.19.088e-1146.88SMESG000067729.1[more]
SMESG000045286.11.184e-954.35SMESG000045286.1[more]
SMESG000079648.11.367e-943.10SMESG000079648.1[more]
SMESG000019274.11.996e-948.21SMESG000019274.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011397 ID=SMED30011397|Name=helix-loop-helix protein 1|organism=Schmidtea mediterranea sexual|type=transcript|length=431bp
GACAATTTGAGTTTAATAAATGAAAATAAATTCATTTAATTCTGTTTTGA
GAAATGAACAAAATGAAATACGATTATGTAAACGATATAAATTCCAGTAC
AAATTCATCTGACAGTTGGATTATTGATAACAACGCTCATTTTCCTGATC
AAGATGAATCGTGGTCGAAATTGAGCAAAGAAGAACGACGACGACGCCGG
CGATCAACGCAAAAATATCGCAATGCTCATGCTGCTCGAGAACGGATACG
AGTTGAAGCATTCAATTCTGCCTTTATCCAATTGAGAAGTCTCCTGCCGA
CATTTCCACCAGACAAGAAAATGTCTAAAATCGAAATTCTACGTCTTGCG
ATTCGATATATGTCCTACTTAGATTTCGTGATTGCCACACAATAACCCCC
CTCGGAAATTTATACCTGAACATAATACAAC
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protein sequence of SMED30011397-orf-1

>SMED30011397-orf-1 ID=SMED30011397-orf-1|Name=SMED30011397-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=114bp
MNKMKYDYVNDINSSTNSSDSWIIDNNAHFPDQDESWSKLSKEERRRRRR
STQKYRNAHAARERIRVEAFNSAFIQLRSLLPTFPPDKKMSKIEILRLAI
RYMSYLDFVIATQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0000418head
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR040238TAL-like
IPR011598bHLH_dom
Vocabulary: molecular function
TermDefinition
GO:0046983protein dimerization activity
GO:0000977RNA polymerase II regulatory region sequence-specific DNA binding
GO:0000981RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0001102RNA polymerase II activating transcription factor binding
GO:0003677DNA binding
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0006351transcription, DNA-templated
GO:0006355regulation of transcription, DNA-templated
GO:0006357regulation of transcription from RNA polymerase II promoter
GO:0007275multicellular organism development
GO:0007617mating behavior
GO:0030154cell differentiation
GO:0042698ovulation cycle
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainSMARTSM00353finuluscoord: 60..112
e-value: 3.6E-16
score: 69.7
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 56..106
e-value: 4.0E-18
score: 65.1
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 54..106
score: 16.71
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 54..106
e-value: 1.31768E-17
score: 68.7799
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 49..113
e-value: 1.1E-20
score: 74.9
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 53..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 34..60
NoneNo IPR availablePANTHERPTHR13864:SF10NESCIENT HELIX LOOP HELIX 2coord: 21..110
IPR040238T-cell acute lymphocytic leukemia protein-likePANTHERPTHR13864T-CELL ACUTE LYMPHOCYTIC LEUKEMIA/STEM CELL LEUKEMIA-RELATEDcoord: 21..110