NEDD8

Overview
NameNEDD8
Smed IDSMED30011383
Length (bp)1028
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of NEDD8 (SMED30011383) t-SNE clustered cells

Violin plots show distribution of expression levels for NEDD8 (SMED30011383) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of NEDD8 (SMED30011383) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for NEDD8 (SMED30011383) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011383

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pharynxSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30011383 dd_Smed_v4_403_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011383 dd_Smed_v6_403_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of NEDD8 vs. Ensembl Human
Match: NEDD8 (NEDD8 ubiquitin like modifier [Source:HGNC Symbol;Acc:HGNC:7732])

HSP 1 Score: 120.553 bits (301), Expect = 1.227e-33
Identity = 69/78 (88.46%), Postives = 74/78 (94.87%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGG 78          
BLAST of NEDD8 vs. Ensembl Human
Match: NEDD8 (NEDD8 ubiquitin like modifier [Source:HGNC Symbol;Acc:HGNC:7732])

HSP 1 Score: 120.553 bits (301), Expect = 1.227e-33
Identity = 69/78 (88.46%), Postives = 74/78 (94.87%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGG 78          
BLAST of NEDD8 vs. Ensembl Human
Match: NEDD8-MDP1 (NEDD8-MDP1 readthrough [Source:HGNC Symbol;Acc:HGNC:39551])

HSP 1 Score: 93.5893 bits (231), Expect = 2.454e-23
Identity = 55/62 (88.71%), Postives = 60/62 (96.77%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKII 245
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIL 62          
BLAST of NEDD8 vs. Ensembl Human
Match: NEDD8-MDP1 (NEDD8-MDP1 readthrough [Source:HGNC Symbol;Acc:HGNC:39551])

HSP 1 Score: 93.5893 bits (231), Expect = 2.454e-23
Identity = 55/62 (88.71%), Postives = 60/62 (96.77%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKII 245
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIL 62          
BLAST of NEDD8 vs. Ensembl Human
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463])

HSP 1 Score: 80.4925 bits (197), Expect = 2.354e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Celegans
Match: ned-8 (NEDD8 [Source:UniProtKB/Swiss-Prot;Acc:Q93725])

HSP 1 Score: 114.775 bits (286), Expect = 6.451e-32
Identity = 64/76 (84.21%), Postives = 72/76 (94.74%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            MLIKVKTLTGKEIE+DIEP D+VERIKE++EEKEGIPP QQRLIF+GKQMNDDK  A+YK++GGSVLHLVLALRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          
BLAST of NEDD8 vs. Ensembl Celegans
Match: ubq-2 (Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ribosomal protein L40 [Source:UniProtKB/Swiss-Prot;Acc:P49632])

HSP 1 Score: 77.0258 bits (188), Expect = 3.860e-17
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71])

HSP 1 Score: 77.411 bits (189), Expect = 2.564e-15
Identity = 43/77 (55.84%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG+
Sbjct:  761 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGD 837          

HSP 2 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 4 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 5 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          

HSP 6 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  305 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380          

HSP 7 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  457 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 532          

HSP 8 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  533 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 608          

HSP 9 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  609 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 684          

HSP 10 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  685 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 760          

HSP 11 Score: 73.9442 bits (180), Expect = 3.728e-14
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTL GK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  381 MQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 456          
BLAST of NEDD8 vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71])

HSP 1 Score: 77.411 bits (189), Expect = 2.564e-15
Identity = 43/77 (55.84%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG+
Sbjct:  761 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGD 837          

HSP 2 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 4 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 5 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          

HSP 6 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  305 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380          

HSP 7 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  457 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 532          

HSP 8 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  533 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 608          

HSP 9 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  609 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 684          

HSP 10 Score: 76.6406 bits (187), Expect = 4.472e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  685 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 760          

HSP 11 Score: 73.9442 bits (180), Expect = 3.728e-14
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTL GK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  381 MQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 456          
BLAST of NEDD8 vs. Ensembl Celegans
Match: ubq-1 (Polyubiquitin-A Ubiquitin Ubiquitin-related [Source:UniProtKB/Swiss-Prot;Acc:P0CG71])

HSP 1 Score: 76.6406 bits (187), Expect = 3.500e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 2 Score: 76.6406 bits (187), Expect = 3.500e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 3 Score: 76.6406 bits (187), Expect = 3.500e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 4 Score: 76.6406 bits (187), Expect = 3.500e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          

HSP 5 Score: 76.6406 bits (187), Expect = 3.500e-15
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  305 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380          

HSP 6 Score: 73.9442 bits (180), Expect = 2.939e-14
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTL GK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  381 MQIFVKTLIGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 456          
BLAST of NEDD8 vs. Ensembl Fly
Match: Nedd8 (gene:FBgn0032725 transcript:FBtr0342928)

HSP 1 Score: 122.865 bits (307), Expect = 1.000e-34
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDS 78          
BLAST of NEDD8 vs. Ensembl Fly
Match: Nedd8 (gene:FBgn0032725 transcript:FBtr0081192)

HSP 1 Score: 122.865 bits (307), Expect = 1.000e-34
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDS 78          
BLAST of NEDD8 vs. Ensembl Fly
Match: RpL40 (gene:FBgn0003941 transcript:FBtr0334787)

HSP 1 Score: 80.4925 bits (197), Expect = 2.559e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Fly
Match: RpL40 (gene:FBgn0003941 transcript:FBtr0077470)

HSP 1 Score: 80.4925 bits (197), Expect = 2.559e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Fly
Match: RpS27A (gene:FBgn0003942 transcript:FBtr0080016)

HSP 1 Score: 80.1073 bits (196), Expect = 7.854e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Zebrafish
Match: nedd8l (NEDD8 ubiquitin like modifier, like [Source:ZFIN;Acc:ZDB-GENE-040718-295])

HSP 1 Score: 119.398 bits (298), Expect = 1.907e-33
Identity = 69/77 (89.61%), Postives = 73/77 (94.81%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG 
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGGE 77          
BLAST of NEDD8 vs. Ensembl Zebrafish
Match: nedd8 (NEDD8 ubiquitin like modifier [Source:ZFIN;Acc:ZDB-GENE-030616-588])

HSP 1 Score: 119.398 bits (298), Expect = 3.058e-33
Identity = 69/77 (89.61%), Postives = 73/77 (94.81%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG 
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGGQ 77          
BLAST of NEDD8 vs. Ensembl Zebrafish
Match: nedd8l (NEDD8 ubiquitin like modifier, like [Source:ZFIN;Acc:ZDB-GENE-040718-295])

HSP 1 Score: 110.538 bits (275), Expect = 9.694e-30
Identity = 64/74 (86.49%), Postives = 69/74 (93.24%), Query Frame = 3
Query:   66 IKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            I  +TLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG
Sbjct:   19 ITSQTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGG 92          
BLAST of NEDD8 vs. Ensembl Zebrafish
Match: ubb (ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10])

HSP 1 Score: 79.7221 bits (195), Expect = 2.111e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Zebrafish
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:ZFIN;Acc:ZDB-GENE-051023-7])

HSP 1 Score: 80.4925 bits (197), Expect = 3.575e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Xenopus
Match: nedd8 (neural precursor cell expressed, developmentally down-regulated 8 [Source:NCBI gene;Acc:549727])

HSP 1 Score: 119.398 bits (298), Expect = 2.895e-33
Identity = 69/76 (90.79%), Postives = 73/76 (96.05%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGG 76          
BLAST of NEDD8 vs. Ensembl Xenopus
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:448705])

HSP 1 Score: 80.4925 bits (197), Expect = 5.252e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Xenopus
Match: rps27a (ribosomal protein S27a [Source:NCBI gene;Acc:548926])

HSP 1 Score: 80.4925 bits (197), Expect = 1.066e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Xenopus
Match: ENSXETT00000033679.1 (ubiquitin C [Source:NCBI gene;Acc:448120])

HSP 1 Score: 79.7221 bits (195), Expect = 7.589e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 2 Score: 79.7221 bits (195), Expect = 7.589e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 3 Score: 79.7221 bits (195), Expect = 7.589e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          
BLAST of NEDD8 vs. Ensembl Xenopus
Match: pdcd11 (programmed cell death 11 [Source:Xenbase;Acc:XB-GENE-967218])

HSP 1 Score: 82.4185 bits (202), Expect = 1.334e-16
Identity = 45/77 (58.44%), Postives = 61/77 (79.22%), Query Frame = 3
Query:   57 VMLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            +M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D +++A+Y I   S LHLVL LRGG
Sbjct:  424 IMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDCRSLADYNIQKESTLHLVLRLRGG 500          

HSP 2 Score: 80.1073 bits (196), Expect = 6.642e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 80.1073 bits (196), Expect = 6.642e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 4 Score: 80.1073 bits (196), Expect = 6.642e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 5 Score: 80.1073 bits (196), Expect = 6.642e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          

HSP 6 Score: 80.1073 bits (196), Expect = 6.642e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  305 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380          

HSP 7 Score: 79.7221 bits (195), Expect = 8.351e-16
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + + +Y I   S LHLVL LRGG
Sbjct:  577 MQIFVKTLTGKTITLEVEPSDTIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGG 652          

HSP 8 Score: 79.337 bits (194), Expect = 1.284e-15
Identity = 44/76 (57.89%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + + +Y I   S LHLVL LRGG
Sbjct:  501 MQIFVKTLTGKTITLEVEPSDTIENVKGKIQDKEGIPPDQQRLIFAGKQLEDGRTLCDYNIQKESTLHLVLRLRGG 576          
BLAST of NEDD8 vs. Ensembl Mouse
Match: Nedd8 (neural precursor cell expressed, developmentally down-regulated gene 8 [Source:MGI Symbol;Acc:MGI:97301])

HSP 1 Score: 105.916 bits (263), Expect = 3.470e-28
Identity = 61/68 (89.71%), Postives = 66/68 (97.06%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLH 263
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLH
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLH 68          
BLAST of NEDD8 vs. Ensembl Mouse
Match: Nedd8 (neural precursor cell expressed, developmentally down-regulated gene 8 [Source:MGI Symbol;Acc:MGI:97301])

HSP 1 Score: 105.916 bits (263), Expect = 3.470e-28
Identity = 61/68 (89.71%), Postives = 66/68 (97.06%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLH 263
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLH
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLH 68          
BLAST of NEDD8 vs. Ensembl Mouse
Match: Gm8797 (predicted pseudogene 8797 [Source:MGI Symbol;Acc:MGI:3643769])

HSP 1 Score: 79.7221 bits (195), Expect = 1.643e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Mouse
Match: Uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:MGI Symbol;Acc:MGI:98887])

HSP 1 Score: 80.4925 bits (197), Expect = 1.845e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Mouse
Match: Gm11808 (predicted gene 11808 [Source:MGI Symbol;Acc:MGI:3649356])

HSP 1 Score: 80.4925 bits (197), Expect = 3.977e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. UniProt/SwissProt
Match: sp|Q9VJ33|NEDD8_DROME (NEDD8 OS=Drosophila melanogaster OX=7227 GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 1.003e-33
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDS 78          
BLAST of NEDD8 vs. UniProt/SwissProt
Match: sp|Q4PLJ0|NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus OX=9986 GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.891e-33
Identity = 69/78 (88.46%), Postives = 74/78 (94.87%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGG 78          
BLAST of NEDD8 vs. UniProt/SwissProt
Match: sp|Q15843|NEDD8_HUMAN (NEDD8 OS=Homo sapiens OX=9606 GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.891e-33
Identity = 69/78 (88.46%), Postives = 74/78 (94.87%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGG 78          
BLAST of NEDD8 vs. UniProt/SwissProt
Match: sp|P61282|NEDD8_BOVIN (NEDD8 OS=Bos taurus OX=9913 GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 5.891e-33
Identity = 69/78 (88.46%), Postives = 74/78 (94.87%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI+GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGG 78          
BLAST of NEDD8 vs. UniProt/SwissProt
Match: sp|Q93725|NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans OX=6239 GN=ned-8 PE=1 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 8.206e-31
Identity = 64/76 (84.21%), Postives = 72/76 (94.74%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            MLIKVKTLTGKEIE+DIEP D+VERIKE++EEKEGIPP QQRLIF+GKQMNDDK  A+YK++GGSVLHLVLALRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76          
BLAST of NEDD8 vs. TrEMBL
Match: A0A484BGE8 (Ubiquitin-like domain-containing protein OS=Drosophila navojoa OX=7232 GN=AWZ03_005793 PE=4 SV=1)

HSP 1 Score: 124.02 bits (310), Expect = 1.064e-31
Identity = 70/77 (90.91%), Postives = 74/77 (96.10%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGGN
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGN 77          
BLAST of NEDD8 vs. TrEMBL
Match: A0A3B0JBK2 (Blast:NEDD8 OS=Drosophila guanche OX=7266 GN=DGUA_6G012258 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.104e-31
Identity = 70/78 (89.74%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG+A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGHA 78          
BLAST of NEDD8 vs. TrEMBL
Match: B4I5M6 (GM17051 OS=Drosophila sechellia OX=7238 GN=Dsec\GM17051 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.138e-31
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGDS 78          
BLAST of NEDD8 vs. TrEMBL
Match: A0A1S3DU76 (NEDD8 OS=Diaphorina citri OX=121845 GN=LOC103524748 PE=4 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.224e-31
Identity = 70/78 (89.74%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLIFSGKQMND+K  AEYK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIFSGKQMNDEKTAAEYKVQGGSVLHLVLALRGGHS 78          
BLAST of NEDD8 vs. TrEMBL
Match: Q29LT5 (Ubiquitin-like domain-containing protein OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=Dpse\GA10488 PE=4 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 2.763e-31
Identity = 69/78 (88.46%), Postives = 75/78 (96.15%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKV+RIKER+EEKEGIPPQQQRLIFSGKQMNDDK  A+YK+ GGSVLHLVLALRGG++
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVDRIKERVEEKEGIPPQQQRLIFSGKQMNDDKTAADYKVQGGSVLHLVLALRGGHS 78          
BLAST of NEDD8 vs. Ensembl Cavefish
Match: nedd8 (NEDD8 [Source:NCBI gene;Acc:103040682])

HSP 1 Score: 119.783 bits (299), Expect = 1.478e-33
Identity = 69/78 (88.46%), Postives = 73/78 (93.59%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNA 293
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG  
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGGRG 78          
BLAST of NEDD8 vs. Ensembl Cavefish
Match: nedd8l (NEDD8-like [Source:NCBI gene;Acc:103044361])

HSP 1 Score: 118.242 bits (295), Expect = 5.888e-33
Identity = 68/77 (88.31%), Postives = 73/77 (94.81%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            MLIKVKTLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  ++YKI GGSVLHLVLALRGG 
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTASDYKIQGGSVLHLVLALRGGR 77          
BLAST of NEDD8 vs. Ensembl Cavefish
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:103027541])

HSP 1 Score: 80.4925 bits (197), Expect = 3.030e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Cavefish
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:103027541])

HSP 1 Score: 80.4925 bits (197), Expect = 3.030e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Cavefish
Match: rps27a (ribosomal protein S27a [Source:NCBI gene;Acc:103044043])

HSP 1 Score: 80.4925 bits (197), Expect = 6.733e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Sea Lamprey
Match: UBB (ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463])

HSP 1 Score: 84.3445 bits (207), Expect = 2.093e-18
Identity = 45/85 (52.94%), Postives = 64/85 (75.29%), Query Frame = 3
Query:   33 VFFIYTFIVMLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            V +   +++M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    5 VRYCKHYLIMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 89          

HSP 2 Score: 80.1073 bits (196), Expect = 6.037e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   90 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 165          

HSP 3 Score: 80.1073 bits (196), Expect = 6.037e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  166 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 241          

HSP 4 Score: 80.1073 bits (196), Expect = 6.037e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  242 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 317          

HSP 5 Score: 80.1073 bits (196), Expect = 6.037e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  318 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 393          
BLAST of NEDD8 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007011.1 (pep scaffold:Pmarinus_7.0:GL484716:14406:16135:-1 gene:ENSPMAG00000006335.1 transcript:ENSPMAT00000007011.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 53.1434 bits (126), Expect = 3.480e-9
Identity = 25/42 (59.52%), Postives = 31/42 (73.81%), Query Frame = 3
Query:  162 GIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            GIPP QQR IF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 GIPPDQQR-IFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 41          
BLAST of NEDD8 vs. Ensembl Sea Lamprey
Match: zfand4 (zinc finger, AN1-type domain 4 [Source:ZFIN;Acc:ZDB-GENE-090312-102])

HSP 1 Score: 53.5286 bits (127), Expect = 4.253e-8
Identity = 34/89 (38.20%), Postives = 51/89 (57.30%), Query Frame = 3
Query:   24 SILVFFIYTFI-VMLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            S  V + + F   M + ++TLTG   EI + P + V  +K +I+  EGIP  QQ LI+   ++ DD  + +Y I  G  + LVLA+RGG
Sbjct:   15 SPYVSYSFPFYETMEVLIETLTGTFFEIRVSPFESVMSVKAKIQRLEGIPITQQHLIWRNIELEDDYCLHDYHITHGCTIKLVLAMRGG 103          
BLAST of NEDD8 vs. Ensembl Yeast
Match: RUB1 (Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme) [Source:SGD;Acc:S000002546])

HSP 1 Score: 79.337 bits (194), Expect = 2.698e-19
Identity = 46/77 (59.74%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M++KVKTLTGKEI ++++ +D V  IKE +EEKEGIPP QQRLIF GKQ++D   V +  ++ G  LHLVL LRGGN
Sbjct:    1 MIVKVKTLTGKEISVELKESDLVYHIKELLEEKEGIPPSQQRLIFQGKQIDDKLTVTDAHLVEGMQLHLVLTLRGGN 77          
BLAST of NEDD8 vs. Ensembl Yeast
Match: RPL40B (Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication [Source:SGD;Acc:S000001802])

HSP 1 Score: 77.0258 bits (188), Expect = 7.742e-18
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Yeast
Match: RPL40A (Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000001410])

HSP 1 Score: 77.0258 bits (188), Expect = 7.742e-18
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Yeast
Match: UBI4 (Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-26S proteasome system; essential for the cellular stress response; encoded as a polyubiquitin precursor comprised of 5 head-to-tail repeats; protein abundance increases in response to DNA replication stress [Source:SGD;Acc:S000003962])

HSP 1 Score: 79.337 bits (194), Expect = 4.919e-17
Identity = 43/77 (55.84%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGGN
Sbjct:  305 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGN 381          

HSP 2 Score: 76.6406 bits (187), Expect = 3.756e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 76.6406 bits (187), Expect = 3.756e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 4 Score: 76.6406 bits (187), Expect = 3.756e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 5 Score: 76.6406 bits (187), Expect = 3.756e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          
BLAST of NEDD8 vs. Ensembl Yeast
Match: RPS31 (Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog [Source:SGD;Acc:S000004157])

HSP 1 Score: 67.3958 bits (163), Expect = 3.039e-14
Identity = 37/70 (52.86%), Postives = 53/70 (75.71%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLV 269
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLV
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLV 70          
BLAST of NEDD8 vs. Ensembl Nematostella
Match: EDO45561 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RSG9])

HSP 1 Score: 80.4925 bits (197), Expect = 1.567e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 2 Score: 50.0618 bits (118), Expect = 1.221e-7
Identity = 31/71 (43.66%), Postives = 50/71 (70.42%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVL 272
            M I VK    K   +D+E +D VE +KE+I+ +EGIPP+ QRL++  +++ D++++A+Y I  GS+LHL L
Sbjct:   77 MQISVKA-HWKTFTLDVEASDTVESVKEKIQNREGIPPKVQRLLYEEEELVDNRSLADYNIKQGSILHLFL 146          
BLAST of NEDD8 vs. Ensembl Nematostella
Match: EDO48503 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RJ25])

HSP 1 Score: 80.1073 bits (196), Expect = 2.757e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Nematostella
Match: EDO32403 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SV54])

HSP 1 Score: 80.8777 bits (198), Expect = 7.035e-18
Identity = 44/77 (57.14%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQR IF+GKQ+ D + +++Y I   S LHLVL LRGGN
Sbjct:  153 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRFIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGN 229          

HSP 2 Score: 79.7221 bits (195), Expect = 1.837e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 79.7221 bits (195), Expect = 1.837e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          
BLAST of NEDD8 vs. Ensembl Nematostella
Match: EDO32547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SUP6])

HSP 1 Score: 82.4185 bits (202), Expect = 2.292e-17
Identity = 45/77 (58.44%), Postives = 60/77 (77.92%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGGN
Sbjct:  457 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGN 533          

HSP 2 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 3 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          

HSP 4 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  153 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 228          

HSP 5 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  229 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 304          

HSP 6 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  305 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 380          

HSP 7 Score: 79.7221 bits (195), Expect = 1.718e-16
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:  381 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 456          
BLAST of NEDD8 vs. Ensembl Nematostella
Match: EDO31255 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYF9])

HSP 1 Score: 63.1586 bits (152), Expect = 5.568e-13
Identity = 35/65 (53.85%), Postives = 51/65 (78.46%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGS 254
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I  G+
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKGN 65          
BLAST of NEDD8 vs. Ensembl Medaka
Match: ENSORLT00000033696.1 (pep primary_assembly:ASM223467v1:18:18100789:18102949:1 gene:ENSORLG00000024017.1 transcript:ENSORLT00000033696.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 1.397e-29
Identity = 66/76 (86.84%), Postives = 71/76 (93.42%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M IK+ TLTGKEIEIDIEPTDKVERIKER+EEKEGIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG
Sbjct:    1 MYIKL-TLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGG 75          
BLAST of NEDD8 vs. Ensembl Medaka
Match: ENSORLT00000041155.1 (pep primary_assembly:ASM223467v1:17:17794475:17794948:1 gene:ENSORLG00000027923.1 transcript:ENSORLT00000041155.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 81.2629 bits (199), Expect = 5.434e-19
Identity = 36/43 (83.72%), Postives = 40/43 (93.02%), Query Frame = 3
Query:  162 GIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            GIPPQQQRLI+SGKQMND+K  A+YKI GGSVLHLVLALRGG+
Sbjct:   38 GIPPQQQRLIYSGKQMNDEKTAADYKIQGGSVLHLVLALRGGS 80          
BLAST of NEDD8 vs. Ensembl Medaka
Match: rps27a (ribosomal protein S27a [Source:NCBI gene;Acc:101164670])

HSP 1 Score: 80.1073 bits (196), Expect = 7.327e-18
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Medaka
Match: rps27a (ribosomal protein S27a [Source:NCBI gene;Acc:101164670])

HSP 1 Score: 80.4925 bits (197), Expect = 1.161e-17
Identity = 44/76 (57.89%), Postives = 59/76 (77.63%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++EP+D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Ensembl Medaka
Match: uba52 (ubiquitin A-52 residue ribosomal protein fusion product 1 [Source:NCBI gene;Acc:101169681])

HSP 1 Score: 77.0258 bits (188), Expect = 5.534e-17
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVEASDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Planmine SMEST
Match: SMESG000035681.1 (SMESG000035681.1)

HSP 1 Score: 77.7962 bits (190), Expect = 2.210e-17
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDSIENVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Planmine SMEST
Match: SMESG000066693.1 (SMESG000066693.1)

HSP 1 Score: 77.411 bits (189), Expect = 3.083e-17
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D +E +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          
BLAST of NEDD8 vs. Planmine SMEST
Match: SMESG000035995.1 (SMESG000035995.1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.821e-17
Identity = 42/77 (54.55%), Postives = 59/77 (76.62%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++EP+D +E +K +I+ KEG+PP QQRLI++GKQ+ND+  + E+ +  GS LHL L L+GGN
Sbjct:  134 MQIFVKTLTGKTITLEVEPSDTIESVKVKIQRKEGVPPVQQRLIWAGKQLNDNHTLLEFNVRKGSTLHLALRLKGGN 210          

HSP 2 Score: 64.6994 bits (156), Expect = 4.252e-11
Identity = 35/74 (47.30%), Postives = 50/74 (67.57%), Query Frame = 3
Query:   66 IKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            I VKTL GK   +++   D +E++K + ++ EG PP QQRLIF+GKQ+ D + +++Y I   S LHL L LRGG
Sbjct:   60 IFVKTLNGKTNTLEVASGDTIEKVKAKSQDNEGFPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLGLRLRGG 133          
BLAST of NEDD8 vs. Planmine SMEST
Match: SMESG000058166.1 (SMESG000058166.1)

HSP 1 Score: 82.4185 bits (202), Expect = 7.231e-17
Identity = 42/77 (54.55%), Postives = 58/77 (75.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGN 290
            M I VKTLTGK I +++EP+D +E +K  I+ KEG+PP QQRLI++GKQ+ND+  + E+ +  GS LHL L L+GGN
Sbjct:  428 MQIFVKTLTGKTITLEVEPSDTIESVKVMIQRKEGVPPVQQRLIWAGKQLNDNHTLLEFNVRKGSTLHLALRLKGGN 504          

HSP 2 Score: 66.6254 bits (161), Expect = 9.708e-12
Identity = 37/74 (50.00%), Postives = 52/74 (70.27%), Query Frame = 3
Query:   66 IKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            I VKTL GK I +++   D ++++K +I++KEGIPP QQRLIF+GKQ+ D   +++Y I     LHL L LRGG
Sbjct:  354 IFVKTLNGKTITLEVASGDTIKKVKAKIQDKEGIPPNQQRLIFTGKQLEDGHTLSDYNIRKEPTLHLGLQLRGG 427          
BLAST of NEDD8 vs. Planmine SMEST
Match: SMESG000025683.1 (SMESG000025683.1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.008e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:    1 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 76          

HSP 2 Score: 76.6406 bits (187), Expect = 1.008e-16
Identity = 42/76 (55.26%), Postives = 58/76 (76.32%), Query Frame = 3
Query:   60 MLIKVKTLTGKEIEIDIEPTDKVXXXXXXXXXXXGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGG 287
            M I VKTLTGK I +++E +D ++ +K +I++KEGIPP QQRLIF+GKQ+ D + +++Y I   S LHLVL LRGG
Sbjct:   77 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG 152          
The following BLAST results are available for this feature:
BLAST of NEDD8 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
NEDD81.227e-3388.46NEDD8 ubiquitin like modifier [Source:HGNC Symbol;... [more]
NEDD81.227e-3388.46NEDD8 ubiquitin like modifier [Source:HGNC Symbol;... [more]
NEDD8-MDP12.454e-2388.71NEDD8-MDP1 readthrough [Source:HGNC Symbol;Acc:HGN... [more]
NEDD8-MDP12.454e-2388.71NEDD8-MDP1 readthrough [Source:HGNC Symbol;Acc:HGN... [more]
UBB2.354e-1857.89ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463][more]
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BLAST of NEDD8 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ned-86.451e-3284.21NEDD8 [Source:UniProtKB/Swiss-Prot;Acc:Q93725][more]
ubq-23.860e-1756.58Ubiquitin-60S ribosomal protein L40 Ubiquitin 60S ... [more]
ubq-12.564e-1555.84Polyubiquitin-A Ubiquitin Ubiquitin-related [Sourc... [more]
ubq-12.564e-1555.84Polyubiquitin-A Ubiquitin Ubiquitin-related [Sourc... [more]
ubq-13.500e-1556.58Polyubiquitin-A Ubiquitin Ubiquitin-related [Sourc... [more]
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BLAST of NEDD8 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nedd81.000e-3488.46gene:FBgn0032725 transcript:FBtr0342928[more]
Nedd81.000e-3488.46gene:FBgn0032725 transcript:FBtr0081192[more]
RpL402.559e-1857.89gene:FBgn0003941 transcript:FBtr0334787[more]
RpL402.559e-1857.89gene:FBgn0003941 transcript:FBtr0077470[more]
RpS27A7.854e-1857.89gene:FBgn0003942 transcript:FBtr0080016[more]
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BLAST of NEDD8 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nedd8l1.907e-3389.61NEDD8 ubiquitin like modifier, like [Source:ZFIN;A... [more]
nedd83.058e-3389.61NEDD8 ubiquitin like modifier [Source:ZFIN;Acc:ZDB... [more]
nedd8l9.694e-3086.49NEDD8 ubiquitin like modifier, like [Source:ZFIN;A... [more]
ubb2.111e-1857.89ubiquitin B [Source:ZFIN;Acc:ZDB-GENE-050411-10][more]
uba523.575e-1857.89ubiquitin A-52 residue ribosomal protein fusion pr... [more]
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BLAST of NEDD8 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nedd82.895e-3390.79neural precursor cell expressed, developmentally d... [more]
uba525.252e-1857.89ubiquitin A-52 residue ribosomal protein fusion pr... [more]
rps27a1.066e-1757.89ribosomal protein S27a [Source:NCBI gene;Acc:54892... [more]
ENSXETT00000033679.17.589e-1757.89ubiquitin C [Source:NCBI gene;Acc:448120][more]
pdcd111.334e-1658.44programmed cell death 11 [Source:Xenbase;Acc:XB-GE... [more]
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BLAST of NEDD8 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Nedd83.470e-2889.71neural precursor cell expressed, developmentally d... [more]
Nedd83.470e-2889.71neural precursor cell expressed, developmentally d... [more]
Gm87971.643e-1857.89predicted pseudogene 8797 [Source:MGI Symbol;Acc:M... [more]
Uba521.845e-1857.89ubiquitin A-52 residue ribosomal protein fusion pr... [more]
Gm118083.977e-1857.89predicted gene 11808 [Source:MGI Symbol;Acc:MGI:36... [more]
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BLAST of NEDD8 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9VJ33|NEDD8_DROME1.003e-3388.46NEDD8 OS=Drosophila melanogaster OX=7227 GN=Nedd8 ... [more]
sp|Q4PLJ0|NEDD8_RABIT5.891e-3388.46NEDD8 OS=Oryctolagus cuniculus OX=9986 GN=NEDD8 PE... [more]
sp|Q15843|NEDD8_HUMAN5.891e-3388.46NEDD8 OS=Homo sapiens OX=9606 GN=NEDD8 PE=1 SV=1[more]
sp|P61282|NEDD8_BOVIN5.891e-3388.46NEDD8 OS=Bos taurus OX=9913 GN=NEDD8 PE=3 SV=1[more]
sp|Q93725|NEDD8_CAEEL8.206e-3184.21NEDD8 OS=Caenorhabditis elegans OX=6239 GN=ned-8 P... [more]
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BLAST of NEDD8 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A484BGE81.064e-3190.91Ubiquitin-like domain-containing protein OS=Drosop... [more]
A0A3B0JBK21.104e-3189.74Blast:NEDD8 OS=Drosophila guanche OX=7266 GN=DGUA_... [more]
B4I5M62.138e-3188.46GM17051 OS=Drosophila sechellia OX=7238 GN=Dsec\GM... [more]
A0A1S3DU762.224e-3189.74NEDD8 OS=Diaphorina citri OX=121845 GN=LOC10352474... [more]
Q29LT52.763e-3188.46Ubiquitin-like domain-containing protein OS=Drosop... [more]
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BLAST of NEDD8 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
nedd81.478e-3388.46NEDD8 [Source:NCBI gene;Acc:103040682][more]
nedd8l5.888e-3388.31NEDD8-like [Source:NCBI gene;Acc:103044361][more]
uba523.030e-1857.89ubiquitin A-52 residue ribosomal protein fusion pr... [more]
uba523.030e-1857.89ubiquitin A-52 residue ribosomal protein fusion pr... [more]
rps27a6.733e-1857.89ribosomal protein S27a [Source:NCBI gene;Acc:10304... [more]
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BLAST of NEDD8 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 3
Match NameE-valueIdentityDescription
UBB2.093e-1852.94ubiquitin B [Source:HGNC Symbol;Acc:HGNC:12463][more]
ENSPMAT00000007011.13.480e-959.52pep scaffold:Pmarinus_7.0:GL484716:14406:16135:-1 ... [more]
zfand44.253e-838.20zinc finger, AN1-type domain 4 [Source:ZFIN;Acc:ZD... [more]
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BLAST of NEDD8 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 5
Match NameE-valueIdentityDescription
RUB12.698e-1959.74Ubiquitin-like protein with similarity to mammalia... [more]
RPL40B7.742e-1855.26Ubiquitin-ribosomal 60S subunit protein L40B fusio... [more]
RPL40A7.742e-1855.26Ubiquitin-ribosomal 60S subunit protein L40A fusio... [more]
UBI44.919e-1755.84Ubiquitin; becomes conjugated to proteins, marking... [more]
RPS313.039e-1452.86Fusion protein cleaved to yield ribosomal protein ... [more]
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BLAST of NEDD8 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO455611.567e-1857.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO485032.757e-1857.89Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO324037.035e-1857.14Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO325472.292e-1758.44Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO312555.568e-1353.85Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of NEDD8 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000033696.11.397e-2986.84pep primary_assembly:ASM223467v1:18:18100789:18102... [more]
ENSORLT00000041155.15.434e-1983.72pep primary_assembly:ASM223467v1:17:17794475:17794... [more]
rps27a7.327e-1857.89ribosomal protein S27a [Source:NCBI gene;Acc:10116... [more]
rps27a1.161e-1757.89ribosomal protein S27a [Source:NCBI gene;Acc:10116... [more]
uba525.534e-1756.58ubiquitin A-52 residue ribosomal protein fusion pr... [more]
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BLAST of NEDD8 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000035681.12.210e-1756.58SMESG000035681.1[more]
SMESG000066693.13.083e-1756.58SMESG000066693.1[more]
SMESG000035995.14.821e-1754.55SMESG000035995.1[more]
SMESG000058166.17.231e-1754.55SMESG000058166.1[more]
SMESG000025683.11.008e-1655.26SMESG000025683.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011383 ID=SMED30011383|Name=NEDD8|organism=Schmidtea mediterranea sexual|type=transcript|length=1028bp
TTAAAATTTTAATAAAATTAATTTCTATTTTAGTCTTTTTTATATATACA
TTTATTGTGATGTTGATTAAAGTTAAAACTCTGACTGGTAAAGAAATTGA
AATAGATATAGAACCTACAGATAAGGTGGAGCGAATTAAAGAAAGAATAG
AAGAAAAAGAAGGTATACCACCTCAACAACAACGGTTGATATTTTCTGGG
AAACAAATGAATGATGACAAAGCAGTTGCTGAATATAAAATAATTGGTGG
TTCAGTTCTTCATTTAGTGTTGGCTTTACGAGGTGGTAATGCATTGTCAA
TCATTGAATAAATTGTTATTTTATTGCTAAAATTAATTTTCCTTTTACAA
ATTTCTATCAATTTAAATTTCTTCAGAATTTATTTAATTTAACGGTTTGT
TAGTATCTTCAATATTTTTAATGATTAGTGCAATATAATGATTGTTCTTG
TATAAAGTAAAGTTGGTTTAATTTGAAATTATTTTATTTTGCGTTTGTTA
ATTATATTTTTATGCATCGTTGTTCCTACATTGATAACTTGGTTTAATTT
TCATTTGTTTTTATGTAGTGTTAAGGAAACTAATTTCAAGAATGTGATTG
TTGGTCTTTGTAAAGCTGGAAAGTTATTTTTGGATTTGTATAATTCTTGC
TTACTGTTGTAGGCACTTTAAAAATATTATGCAAGATTTTCCAGTTTAAC
TGAAATTTTGATTTTTACGTATTTTATATACAAGTGTTAAATTCTTGAAC
CAATACAAAATGGAATCAAGTTTTTAATAAAAAGTAACTTAAAATTTCCA
ATGGTTTCATTTAAGATCAAAATGTGAAAATCAACAATCCCATTGAAAAA
AAAGTAGCATAAAAGTTAGATTAATTATCGTTCGACTAAATGAAATTTGT
ATATGAATATTTCAGGAATTTACAAGATAAAAATTTGATGTGATTTTGTG
TTATTATACAATGAATAAGTTGTCGTTTTAGTTTTATTATTATTTTGAAC
CTTATTAATAAATCTTAATAATGTTGGG
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protein sequence of SMED30011383-orf-1

>SMED30011383-orf-1 ID=SMED30011383-orf-1|Name=SMED30011383-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=103bp
KILIKLISILVFFIYTFIVMLIKVKTLTGKEIEIDIEPTDKVERIKERIE
EKEGIPPQQQRLIFSGKQMNDDKAVAEYKIIGGSVLHLVLALRGGNALSI
IE*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000429neoblast
PLANA:0002032epidermal cell
Vocabulary: INTERPRO
TermDefinition
IPR038738Nedd8-like
IPR019956Ubiquitin_dom
IPR000626Ubiquitin-like_dom
IPR019954Ubiquitin_CS
IPR029071Ubiquitin-like_domsf
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0031386protein tag
Vocabulary: cellular component
TermDefinition
GO:0005634nucleus
Vocabulary: biological process
TermDefinition
GO:0007275multicellular organism development
GO:0008283cell proliferation
GO:0008589regulation of smoothened signaling pathway
GO:0022008neurogenesis
GO:0030162regulation of proteolysis
GO:0030431sleep
GO:0031647regulation of protein stability
GO:0036099female germ-line stem cell population maintenance
GO:0045116protein neddylation
GO:0048812neuron projection morphogenesis
GO:0051438regulation of ubiquitin-protein transferase activity
GO:2000736regulation of stem cell differentiation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019956Ubiquitin domainPRINTSPR00348UBIQUITINcoord: 30..50
score: 34.89
coord: 72..93
score: 47.57
coord: 51..71
score: 73.83
IPR000626Ubiquitin-like domainSMARTSM00213ubq_7coord: 20..91
e-value: 1.3E-24
score: 97.8
IPR000626Ubiquitin-like domainPFAMPF00240ubiquitincoord: 22..93
e-value: 4.7E-27
score: 93.5
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 20..95
score: 25.357
NoneNo IPR availableGENE3DG3DSA:3.10.20.90coord: 17..96
e-value: 1.2E-32
score: 113.6
NoneNo IPR availablePANTHERPTHR10666UBIQUITINcoord: 19..98
NoneNo IPR availablePANTHERPTHR10666:SF280NEURAL PRECURSOR CELL-EXPRESSED, DEVELOPMENTALLY DOWN-REGULATED 8coord: 19..98
NoneNo IPR availableTMHMMTMhelixcoord: 2..24
IPR019954Ubiquitin conserved sitePROSITEPS00299UBIQUITIN_1coord: 46..71
IPR038738Nedd8-like ubiquitinCDDcd01806Ubl_NEDD8coord: 22..95
e-value: 1.58279E-47
score: 144.454
IPR029071Ubiquitin-like domain superfamilySUPERFAMILYSSF54236Ubiquitin-likecoord: 18..95