Calcium activated potassium channel subunit

Overview
NameCalcium activated potassium channel subunit
Smed IDSMED30011376
Length (bp)778
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Calcium activated potassium channel subunit (SMED30011376) t-SNE clustered cells

Violin plots show distribution of expression levels for Calcium activated potassium channel subunit (SMED30011376) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Calcium activated potassium channel subunit (SMED30011376) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Calcium activated potassium channel subunit (SMED30011376) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011376

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
nervous systemSMED30011376h1SMcG0020732 dd_Smed_v4_15762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011376h1SMcG0020732 dd_Smed_v4_15762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30011376h1SMcG0020732 dd_Smed_v4_15762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30011376h1SMcG0020732 dd_Smed_v4_15762_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Human
Match: KCNMB4 (potassium calcium-activated channel subfamily M regulatory beta subunit 4 [Source:HGNC Symbol;Acc:HGNC:6289])

HSP 1 Score: 53.9138 bits (128), Expect = 4.072e-8
Identity = 44/170 (25.88%), Postives = 75/170 (44.12%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCN-LNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV---SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLLLIV 669
            SI +  F+++  ++ L I       P L +     A C  L+  ++G+   C   C  D    S++ C+Q+ VN   +N     +  EH    +  +   C+   PC +E   N   V    ++ +D    + F C F   + P + LL++ +D    LHC LWPL+  V
Sbjct:   17 SIRLGLFLIISGVVSLFIFGFCWLSPALQDLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEH----QLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLWPLVTFV 182
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Zebrafish
Match: si:ch211-38m6.7 (si:ch211-38m6.7 [Source:ZFIN;Acc:ZDB-GENE-130603-15])

HSP 1 Score: 51.6026 bits (122), Expect = 2.463e-7
Identity = 44/164 (26.83%), Postives = 74/164 (45.12%), Query Frame = 1 
Query:  223 FIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHV----ATLRHFSKNHCATSP-CHKEEIDNYLIVSRFNRDIKRQR-NFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVI 696
            +++V ILM   + P +H     S  C+L    + DEV     C +    FKCL+ +VN   +NK  S  LY       + L+  S + C  +P C + + +         + +  QR N  CF S  HP +A+  + Y+    L+  LWP L++      + ++
Sbjct:   66 YLMVGILM---VKPCIHSDWISSVNCSLVHIDLSDEVM---DC-RGIVNFKCLRALVNITTSNKTSSLQLYYDEDAVNSRLKTNSFSQCFYTPKCQRNKTEQETEARNIKQALITQRENLTCFLSPSHPEDAICRRKYNLEMALYYLLWPSLMLFGGIVLVGLV 222
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Zebrafish
Match: si:ch211-38m6.7 (si:ch211-38m6.7 [Source:ZFIN;Acc:ZDB-GENE-130603-15])

HSP 1 Score: 51.6026 bits (122), Expect = 2.518e-7
Identity = 44/164 (26.83%), Postives = 74/164 (45.12%), Query Frame = 1 
Query:  223 FIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHV----ATLRHFSKNHCATSP-CHKEEIDNYLIVSRFNRDIKRQR-NFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVI 696
            +++V ILM   + P +H     S  C+L    + DEV     C +    FKCL+ +VN   +NK  S  LY       + L+  S + C  +P C + + +         + +  QR N  CF S  HP +A+  + Y+    L+  LWP L++      + ++
Sbjct:   85 YLMVGILM---VKPCIHSDWISSVNCSLVHIDLSDEVM---DC-RGIVNFKCLRALVNITTSNKTSSLQLYYDEDAVNSRLKTNSFSQCFYTPKCQRNKTEQETEARNIKQALITQRENLTCFLSPSHPEDAICRRKYNLEMALYYLLWPSLMLFGGIVLVGLV 241
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Zebrafish
Match: kcnmb2 (potassium large conductance calcium-activated channel, subfamily M, beta member 2 [Source:ZFIN;Acc:ZDB-GENE-050320-97])

HSP 1 Score: 50.447 bits (119), Expect = 5.250e-7
Identity = 46/200 (23.00%), Postives = 80/200 (40.00%), Query Frame = 1 
Query:  160 ATKENWFKIIYLASISIFVST--FIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCF-TSIKHPSEALLYKFYDASTFLHCTLWP---------LLLIVSSATSLTVI-YQIDK 711
            A K    + I L    +F S   + V+CI M   +  Y          C +  + +  E+ C   C  D    SK+ CLQ+ V+     ++    L  H   +   S        C K+     +++   +  +K Q+   CF    +H +  LL + Y +++ LH   WP         ++L+V     L+++  QIDK
Sbjct:   36 ALKAGEDRAILLGLSMVFSSVMMYFVICITM---VRAYADSLLIDETTCTVLNSSLTKEINCSYNCGSDCWRSSKYPCLQVYVSLNATGRIG---LLSHNEEMLDVSSECFYVPKCQKDHTAMRVLIQNISDHLKMQQQVRCFHDPSEHQASILLKRIYGSASLLHSLFWPSCMLSGGTLIILMVKLTQYLSILCEQIDK 229
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Xenopus
Match: dnajc1 (DnaJ heat shock protein family (Hsp40) member C1 [Source:Xenbase;Acc:XB-GENE-950431])

HSP 1 Score: 53.9138 bits (128), Expect = 3.552e-8
Identity = 52/163 (31.90%), Postives = 73/163 (44.79%), Query Frame = 1 
Query:  202 ISIFVSTFIVVCILMHLVIIPYLHETSFISAQCN-LNETRMGDEVRCENKCS---KDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV----SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLL 660
            IS  +S FI+    +H    P L E    +A C  L+  ++G+   C   C    K  S + CLQI VN   +N    A L++    L   S   C+   PC +E   N   V      +N +I  Q  F C F   + P + LL + +D S  LHC LWPL+
Sbjct:   26 ISGVISLFILGFCWLH----PALLEMQGKTANCTVLSVQQIGETFECTFSCGSECKGTSLYPCLQIYVNNSESNS--RALLHQDERQL--ISNPKCSYIPPCERENQKNSENVLSWQEYWNNEIGFQ-TFTCYFNKHRRPEDVLLRRTHDESVLLHCFLWPLV 179
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Xenopus
Match: serp1 (stress-associated endoplasmic reticulum protein 1 [Source:Xenbase;Acc:XB-GENE-1004958])

HSP 1 Score: 47.7506 bits (112), Expect = 8.042e-6
Identity = 42/182 (23.08%), Postives = 80/182 (43.96%), Query Frame = 1 
Query:  193 LASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSP-------CHKEE---IDNYLIVSRFNRDIKRQRNFNCFTSIK-HPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVI 696
            +  +S+ +   + + IL   ++  +  E++    Q ++    M D V C   C  D   +SK+ CLQ++VN   +N   SA L        H+++     +P       C +++   +D  + +  +  D K    F CF   +  P + +  K YD S   HC  WP L+++  A  + ++
Sbjct:   81 MMGLSVLMFFLLGITILKPFMLSVWNEESNCTIIQADI----MEDWVDCSFTCGVDCHGQSKYPCLQVLVNV--SNSGQSAVL--------HYNEEAVQVNPKCFYIPRCQRDKNDLLDGVMNIKEY-FDRKNDTPFLCFHRPESKPEDVIFIKKYDRSVVFHCLFWPSLMLLGGALIVGMV 247
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Mouse
Match: Kcnmb4 (potassium large conductance calcium-activated channel, subfamily M, beta member 4 [Source:MGI Symbol;Acc:MGI:1913272])

HSP 1 Score: 51.9878 bits (123), Expect = 1.155e-7
Identity = 43/170 (25.29%), Postives = 75/170 (44.12%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCN-LNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV---SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLLLIV 669
            SI +  F++V  ++ L I       P L +    +A C  L+  ++G+   C   C  D    S++ C+Q+ VN   +N     +  +H    +  +   C+   PC +E   N   V    ++ +D    + F C F   + P + LL + +D    LHC LWP++  V
Sbjct:   17 SIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQH----QLLTNPKCSYIPPCKRENQKNSESVMNWQQYWKDEIGSQPFTCYFNQHQRPEDVLLQRTHDEIALLHCFLWPVVAFV 182
[Close]
BLAST of Calcium activated potassium channel subunit vs. UniProt/SwissProt
Match: sp|Q86W47|KCMB4_HUMAN (Calcium-activated potassium channel subunit beta-4 OS=Homo sapiens OX=9606 GN=KCNMB4 PE=1 SV=2)

HSP 1 Score: 53.9138 bits (128), Expect = 1.956e-7
Identity = 44/170 (25.88%), Postives = 75/170 (44.12%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCN-LNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV---SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLLLIV 669
            SI +  F+++  ++ L I       P L +     A C  L+  ++G+   C   C  D    S++ C+Q+ VN   +N     +  EH    +  +   C+   PC +E   N   V    ++ +D    + F C F   + P + LL++ +D    LHC LWPL+  V
Sbjct:   17 SIRLGLFLIISGVVSLFIFGFCWLSPALQDLQATEANCTVLSVQQIGEVFECTFTCGADCRGTSQYPCVQVYVNNSESNSRALLHSDEH----QLLTNPKCSYIPPCKRENQKNLESVMNWQQYWKDEIGSQPFTCYFNQHQRPDDVLLHRTHDEIVLLHCFLWPLVTFV 182
[Close]
BLAST of Calcium activated potassium channel subunit vs. UniProt/SwissProt
Match: sp|Q9JIN6|KCMB4_MOUSE (Calcium-activated potassium channel subunit beta-4 OS=Mus musculus OX=10090 GN=Kcnmb4 PE=1 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 8.078e-7
Identity = 43/170 (25.29%), Postives = 75/170 (44.12%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCN-LNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV---SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLLLIV 669
            SI +  F++V  ++ L I       P L +    +A C  L+  ++G+   C   C  D    S++ C+Q+ VN   +N     +  +H    +  +   C+   PC +E   N   V    ++ +D    + F C F   + P + LL + +D    LHC LWP++  V
Sbjct:   17 SIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQH----QLLTNPKCSYIPPCKRENQKNSESVMNWQQYWKDEIGSQPFTCYFNQHQRPEDVLLQRTHDEIALLHCFLWPVVAFV 182
[Close]
BLAST of Calcium activated potassium channel subunit vs. UniProt/SwissProt
Match: sp|Q9ESK8|KCMB4_RAT (Calcium-activated potassium channel subunit beta-4 OS=Rattus norvegicus OX=10116 GN=Kcnmb4 PE=2 SV=1)

HSP 1 Score: 51.9878 bits (123), Expect = 8.973e-7
Identity = 43/170 (25.29%), Postives = 75/170 (44.12%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCN-LNETRMGDEVRCENKCSKD---RSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV---SRFNRDIKRQRNFNC-FTSIKHPSEALLYKFYDASTFLHCTLWPLLLIV 669
            SI +  F++V  ++ L I       P L +    +A C  L+  ++G+   C   C  D    S++ C+Q+ VN   +N     +  +H    +  +   C+   PC +E   N   V    ++ +D    + F C F   + P + LL + +D    LHC LWP++  V
Sbjct:   17 SIRLGLFLIVSGILSLFIFGFCWLSPALQDLQATAANCTVLSVQQIGEVFECTFTCGTDCRGTSQYPCVQVYVNNSESNSRALLHSDQH----QLLTNPKCSYIPPCKRENQKNSESVMNWQQYWKDEIGSQPFTCYFNQHQRPEDVLLQRTHDEIVLLHCFLWPVVAFV 182
[Close]
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A4Z2CW83 (Calcium-activated potassium channel subunit beta-2 OS=Schistosoma japonicum OX=6182 GN=EWB00_007000 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 2.705e-53
Identity = 89/192 (46.35%), Postives = 123/192 (64.06%), Query Frame = 1 
Query:  172 NWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCN-LNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVI 744
            N  KIIYL   ++  +TFIV CIL+HLV+ PY HE++F    C+ L+   +  +V+CENKCSKDRSKF CL++IV Y   NK  +  L++++AT  H+    CATS CH  ++DN   V +F R I+ QR+F C+    H  EALLYKFY  ++  H   WP+ L + S+  L +IY +DKCRVW  D+ VI
Sbjct:   20 NSIKIIYLTCATVITTTFIVECILIHLVVQPYFHESAFTHTNCSFLHAYIVRTDVKCENKCSKDRSKFPCLKVIVQYLSGNKNHTVILFDNIATYNHYKLLGCATSACHHRDMDNTEWVEKFQRRIRLQRHFRCYVHATHEDEALLYKFYTFTSVFHAVFWPISLFLISSICLLLIYIMDKCRVWTGDQDVI 211
[Close]
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A4E0RG47 (Calcium-activated potassium channel subunit beta-2 OS=Fasciola hepatica OX=6192 GN=D915_003482 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.627e-53
Identity = 84/197 (42.64%), Postives = 128/197 (64.97%), Query Frame = 1 
Query:  160 ATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCN-LNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
            A +E   K+ YL   ++  + FI+ CIL+HLVI+P  +ET F + +C  L  T + + V+CEN+CSKDRS F+C+++IV+Y+++ K F+A L++++AT +H+    CATS CH++   N   V RF + ++R  +F C+T+  H  EALLYKFY  ST  +   WPL L+  S   L V++  D+CRVW DD  VI+
Sbjct:   47 AIREPLIKVTYLTCFTLITTGFIIECILLHLVILPSQYETGFTATECAYLRTTLVMESVKCENRCSKDRSAFQCVRVIVSYKKSGKNFTASLFDNIATYQHYHFLGCATSSCHRQNSANQAWVQRFTQLVRRHGHFTCYTNENHEDEALLYKFYGPSTLFNTIFWPLGLLFLSGMCLMVVWLYDRCRVWGDDNIVIA 243
[Close]
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A504ZCY1 (Calcium-activated potassium channel subunit beta-2 OS=Fasciola gigantica OX=46835 GN=FGIG_06101 PE=4 SV=1)

HSP 1 Score: 181.415 bits (459), Expect = 5.751e-53
Identity = 83/197 (42.13%), Postives = 128/197 (64.97%), Query Frame = 1 
Query:  160 ATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCN-LNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
            A +E   K+ YL   ++  + FIV CIL+HL+I+P  +ET F + +C  L  T + + V+CEN+CSKDRS F+C++++V+Y+++ K F+A L++++AT +H+    CATS CH++   N   V RF + ++R  +F C+T+  H  EALLYKFY  ST  +   WPL L+  S   L V++  D+CRVW DD  VI+
Sbjct:   47 AIREPLIKVTYLTCFTLITTGFIVECILLHLIILPSQYETGFTATECAYLRTTLVMESVKCENRCSKDRSAFQCVRVVVSYKKSGKNFTASLFDNIATYQHYHFLGCATSSCHRQNSANQAWVQRFTQLVRRHGHFTCYTNENHEDEALLYKFYGPSTLFNTIFWPLGLLFLSGMCLMVVWLYDRCRVWGDDNIVIA 243
[Close]
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A504Z4N0 (Calcium activated potassium channel subunit OS=Fasciola gigantica OX=46835 GN=FGIG_02312 PE=4 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.189e-50
Identity = 80/191 (41.88%), Postives = 124/191 (64.92%), Query Frame = 1 
Query:  178 FKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRM-GDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
             K +YL   ++  +T I+ CIL+HLVI+PY HE+ F   +C+  +  +  + V+CENKCSKDRS F+C+++IV+Y +  + ++A L++++AT +H+    CATS CH++   N+  V RF+R I+R   F CFT   H +EALL+KFY  +T  +   WPL L+  S T L +++  D+CRVW D   VI+
Sbjct:   64 LKFVYLTCATLITTTLIIECILLHLVIMPYCHESGFTPTECSYKQAHVVMNAVKCENKCSKDRSSFRCIRVIVSYTKWGRNYTANLFDNIATYQHYHNLGCATSSCHRQNAANHAWVQRFHRMIRRSMKFTCFTHEDHINEALLHKFYGPATLFNTLFWPLGLLGLSTTCLLILWLYDRCRVWGDGNIVIA 254
[Close]
BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Match: A0A4E0RRI8 (Calcium activated potassium channel subunit OS=Fasciola hepatica OX=6192 GN=D915_000502 PE=4 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 1.215e-50
Identity = 80/191 (41.88%), Postives = 123/191 (64.40%), Query Frame = 1 
Query:  178 FKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRM-GDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
             K +YL   ++  +TFI+ CIL+HLVI+PY HE+ F   +C   +  +  + V+CENKCSKDRS F+C++++V+Y +  + ++A L++++AT +H+    CATS CH++   N+  V RF+R I+R   F CFT   H +EALL+KFY  +T  +   WPL L   S T L +++  D+CRVW D   VI+
Sbjct:   64 LKFVYLTCATLITTTFIIECILLHLVIMPYCHESGFTPTECAYKQAHVVMNAVKCENKCSKDRSSFRCIRVVVSYTKWGRNYTANLFDNIATYQHYHNLGCATSSCHRQNAANHAWVQRFHRMIRRSMKFTCFTHEDHINEALLHKFYGPATLFNTLFWPLGLFGLSTTCLLILWLYDRCRVWGDGNIVIA 254
[Close]
BLAST of Calcium activated potassium channel subunit vs. Ensembl Medaka
Match: KCNMB4 (potassium calcium-activated channel subfamily M regulatory beta subunit 4 [Source:NCBI gene;Acc:101171123])

HSP 1 Score: 49.6766 bits (117), Expect = 8.608e-7
Identity = 44/169 (26.04%), Postives = 70/169 (41.42%), Query Frame = 1 
Query:  205 SIFVSTFIVVCILMHLVII------PYLHETSFISAQCNLNETRMGDEV-----RCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCA-TSPCHKEEIDNYLIV----SRFNRDIKRQRNFNCF-TSIKHPSEALLYKFYDASTFLHCTLWPLL 660
            SI +  F++ C ++ L I+      P L       A C +      +E+      C   C K  S + CLQI VN   +N +   +  E    L       C+   PC ++   N   V      F+ ++  Q +F CF    + P + L  + +D S  LHC LWP++
Sbjct:   17 SIRLGLFLIACGILSLFILGFCWLSPTLQSLQSKPANCTVVSVLRPEEMFECVFTCGTDC-KGTSLYPCLQIFVNNSESNSVALLHFDEQQLVLNP----KCSYVPPCERDNQKNKDSVLWWEDYFSNEVSSQ-SFTCFFNQQRRPDDVLWRRSHDTSVLLHCILWPMV 179
[Close]
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000017226.1 (SMESG000017226.1)

HSP 1 Score: 407.912 bits (1047), Expect = 6.248e-146
Identity = 196/196 (100.00%), Postives = 196/196 (100.00%), Query Frame = 1 
Query:  160 ATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
            ATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS
Sbjct:   33 ATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 228
[Close]
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000047387.1 (SMESG000047387.1)

HSP 1 Score: 181.415 bits (459), Expect = 4.831e-57
Identity = 84/187 (44.92%), Postives = 116/187 (62.03%), Query Frame = 1 
Query:  169 ENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHD 729
            ++W K +Y++  ++ +   IV  IL+HLV+IP+ HE SF S+ C L  T +   ++CENKCSKDRS F CLQI   Y   ++ F AY+Y+H+ATL H+ K+ CA SPC     DN +IVS F   +++   F C+ + +H  E LLYKFY    F HC  WP  L+V S   L V+Y  DKC VW+D
Sbjct:   10 QDWVKFLYISCSTLSLCALIVGAILIHLVVIPFTHENSFYSSTCTLINTNITTSIKCENKCSKDRSMFPCLQITAKYTDLDQEFLAYVYDHIATLSHYRKSQCAISPCFSSTKDNGIIVSEFQSRMRKTPTFACYINAEHRDEVLLYKFYSIGMFWHCVAWPSGLLVISLLILAVLYSKDKCNVWND 196
[Close]
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000055809.1 (SMESG000055809.1)

HSP 1 Score: 150.984 bits (380), Expect = 5.927e-45
Identity = 75/202 (37.13%), Postives = 116/202 (57.43%), Query Frame = 1 
Query:  178 FKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYR--------RNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNR----DIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
             K +Y++S+S+ VS FI+ CIL+HL+I PY HET F+  +C +        ++C NKCSK+RSKF C  + V Y+         N   +  YL++ + TL +F    C  SPC+K ++DNY+    F       ++    F C+ +    +E LLYKFY   T++ C LWP+ LIV S   + + Y ID+C+VW +D+ +I+
Sbjct:   13 LKFLYISSLSVAVSGFIIECILLHLIISPYGHETQFVDGKCTVLNISTRYGIKCVNKCSKERSKFFCSTVKVKYQSKISLGRLSNQTWYHGYLFDQITTLHNFKDEKCIISPCYKLKLDNYIETRNFLHVHFDHLESNSAFPCYVNRYLTNEVLLYKFYSYETYISCILWPITLIVLSVFIILLCYWIDRCKVWANDKAIIT 214
[Close]
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000053268.1 (SMESG000053268.1)

HSP 1 Score: 140.584 bits (353), Expect = 7.239e-41
Identity = 74/199 (37.19%), Postives = 108/199 (54.27%), Query Frame = 1 
Query:  181 KIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCNLNETR-----MGD-EVRCENKCSKDRSKFKCLQIIVNYRRNN----KMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS 747
            K+IYL+S++   S FI+ CIL+HL++ PYL E  F    C    +      + D ++RCENKC+KD  KF C+ ++VNY   N    ++   YLY++  T   + K  C+TSPCH    DN   V  F+R +  + NF+C+ + K     LLYK Y  S  LHC LWP  + +     +T    I  C VW ++  VI+
Sbjct:   20 KLIYLSSMTFLTSGFILECILLHLMLTPYLAEQEFEQTTCVYRNSSSRFILLSDLQLRCENKCTKDTGKFPCVWLLVNYVIPNSGQRQLQDGYLYDYYRTYNRYKKQRCSTSPCHLLLSDNIDWVISFDRTLYSKHNFSCYYNTKDHMSVLLYKLYPKSLILHCILWPFGIFIVGLVGITFTALIAGCDVWKNEDVVIA 218
[Close]
BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Match: SMESG000055809.1 (SMESG000055809.1)

HSP 1 Score: 134.42 bits (337), Expect = 5.894e-38
Identity = 75/191 (39.27%), Postives = 109/191 (57.07%), Query Frame = 1 
Query:  178 FKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQCN-LN-ETRMGDEVRCENKCSKDRSKFKCLQIIVNYR--------RNNKMFSAYLYEHVATLRHFSKNHCATSPCHKEEIDNYLIVSRFNR----DIKRQRNFNCFTSIKHPSEALLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQID 708
             K +Y++S+S+ VS FI+ CIL+HL+I PY HET F+  +C  LN  TR G  ++C NKCSK+RSKF C  + V Y+         N   +  YL++ + TL +F    C  SPC+K ++DNY+    F       ++    F C+ +    +E LLYKFY   T++ C LWP+ LIV S   + + Y ID
Sbjct:   13 LKFLYISSLSVAVSGFIIECILLHLIISPYGHETQFVDGKCTVLNISTRYG--IKCVNKCSKERSKFFCSTVKVKYQSKISLGRLSNQTWYHGYLFDQITTLHNFKDEKCIISPCYKLKLDNYIETRNFLHVHFDHLESNSAFPCYVNRYLTNEVLLYKFYSYETYISCILWPITLIVLSVFIILLCYWID 201
The following BLAST results are available for this feature:
BLAST of Calcium activated potassium channel subunit vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
KCNMB44.072e-825.88potassium calcium-activated channel subfamily M re... [more]
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
si:ch211-38m6.72.463e-726.83si:ch211-38m6.7 [Source:ZFIN;Acc:ZDB-GENE-130603-1... [more]
si:ch211-38m6.72.518e-726.83si:ch211-38m6.7 [Source:ZFIN;Acc:ZDB-GENE-130603-1... [more]
kcnmb25.250e-723.00potassium large conductance calcium-activated chan... [more]
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dnajc13.552e-831.90DnaJ heat shock protein family (Hsp40) member C1 [... [more]
serp18.042e-623.08stress-associated endoplasmic reticulum protein 1 ... [more]
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Kcnmb41.155e-725.29potassium large conductance calcium-activated chan... [more]
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BLAST of Calcium activated potassium channel subunit vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q86W47|KCMB4_HUMAN1.956e-725.88Calcium-activated potassium channel subunit beta-4... [more]
sp|Q9JIN6|KCMB4_MOUSE8.078e-725.29Calcium-activated potassium channel subunit beta-4... [more]
sp|Q9ESK8|KCMB4_RAT8.973e-725.29Calcium-activated potassium channel subunit beta-4... [more]
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BLAST of Calcium activated potassium channel subunit vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A4Z2CW832.705e-5346.35Calcium-activated potassium channel subunit beta-2... [more]
A0A4E0RG475.627e-5342.64Calcium-activated potassium channel subunit beta-2... [more]
A0A504ZCY15.751e-5342.13Calcium-activated potassium channel subunit beta-2... [more]
A0A504Z4N01.189e-5041.88Calcium activated potassium channel subunit OS=Fas... [more]
A0A4E0RRI81.215e-5041.88Calcium activated potassium channel subunit OS=Fas... [more]
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Calcium activated potassium channel subunit vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
KCNMB48.608e-726.04potassium calcium-activated channel subfamily M re... [more]
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BLAST of Calcium activated potassium channel subunit vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000017226.16.248e-146100.00SMESG000017226.1[more]
SMESG000047387.14.831e-5744.92SMESG000047387.1[more]
SMESG000055809.15.927e-4537.13SMESG000055809.1[more]
SMESG000053268.17.239e-4137.19SMESG000053268.1[more]
SMESG000055809.15.894e-3839.27SMESG000055809.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011376 ID=SMED30011376|Name=Calcium activated potassium channel subunit|organism=Schmidtea mediterranea sexual|type=transcript|length=778bp
CAAAATCAGAGGAGAAATAATAAATATCCCACAAAATGTTTGCTCTCTCA
ATCCATCTATTGCATGACAACCAACACAAATTCCAATAAGTCATCCGTGT
CCGTTACTGCCTTGCGTCGTCAGTCAACGAGACGGCGTCCAAAACCGTCG
ACCAGTCGAGCGACAAAGGAAAACTGGTTCAAAATTATTTATCTCGCCTC
CATCTCGATATTTGTGTCGACATTCATTGTTGTTTGCATTCTGATGCATC
TGGTTATCATTCCATATCTCCACGAGACGTCTTTCATCTCTGCCCAATGC
AATCTCAATGAGACGCGGATGGGGGACGAGGTGAGATGTGAAAACAAATG
CAGCAAAGACCGATCCAAATTTAAATGTTTACAAATAATTGTCAATTACC
GAAGGAATAACAAAATGTTCTCGGCATATCTTTACGAGCACGTCGCAACC
TTAAGGCATTTCAGCAAAAATCACTGCGCCACAAGTCCTTGCCATAAGGA
GGAAATTGACAATTACTTAATTGTCTCCAGGTTCAACAGAGACATCAAAA
GACAACGAAACTTCAACTGTTTCACCAGCATCAAGCATCCTTCTGAAGCA
CTTCTTTACAAATTCTATGACGCCTCGACATTTCTCCATTGCACCTTGTG
GCCGCTTCTCCTCATAGTCAGTTCTGCTACGTCTCTGACGGTCATTTATC
AGATAGACAAATGCCGCGTTTGGCACGACGACAGGACCGTGATTTCTTGA
TTTTATAATTATTTTATAAATAACTAGC
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protein sequence of SMED30011376-orf-1

>SMED30011376-orf-1 ID=SMED30011376-orf-1|Name=SMED30011376-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=250bp
QNQRRNNKYPTKCLLSQSIYCMTTNTNSNKSSVSVTALRRQSTRRRPKPS
TSRATKENWFKIIYLASISIFVSTFIVVCILMHLVIIPYLHETSFISAQC
NLNETRMGDEVRCENKCSKDRSKFKCLQIIVNYRRNNKMFSAYLYEHVAT
LRHFSKNHCATSPCHKEEIDNYLIVSRFNRDIKRQRNFNCFTSIKHPSEA
LLYKFYDASTFLHCTLWPLLLIVSSATSLTVIYQIDKCRVWHDDRTVIS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
Vocabulary: INTERPRO
TermDefinition
IPR003930K_chnl_Ca-activ_BK_bsu
Vocabulary: biological process
TermDefinition
GO:0006813potassium ion transport
GO:0071805potassium ion transmembrane transport
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: molecular function
TermDefinition
GO:0015269calcium-activated potassium channel activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003930Potassium channel, calcium-activated, BK, beta subunitPFAMPF03185CaKBcoord: 73..224
e-value: 1.9E-17
score: 63.6
IPR003930Potassium channel, calcium-activated, BK, beta subunitPANTHERPTHR10258CALCIUM-ACTIVATED POTASSIUM CHANNEL SUBUNIT BETAcoord: 51..245
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 32..52
NoneNo IPR availableTMHMMTMhelixcoord: 63..85
NoneNo IPR availableTMHMMTMhelixcoord: 211..233