SMED30011316

Overview
NameSMED30011316
Smed IDSMED30011316
Length (bp)3161
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011316 (SMED30011316) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011316 (SMED30011316) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011316 (SMED30011316) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011316 (SMED30011316) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011316

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30011316SMESG000065696.1 SmedASXL_001008SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
X1 cellSMED30011316SMESG000065696.1 SmedASXL_019869SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
zeta neoblastSMED30011316SMESG000065696.1 dd_Smed_v4_12726_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30011316SMESG000065696.1 dd_Smed_v4_39554_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011316SMESG000065696.1 SMED_05003_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30011316SMESG000065696.1 SMED_05003_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011316 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001732:791..5149 -13019
Homology
BLAST of SMED30011316 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1)

HSP 1 Score: 1396.33 bits (3613), Expect = 0.000e+0
Identity = 696/700 (99.43%), Postives = 697/700 (99.57%), Query Frame = 2
Query:  878 KSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFXXXXXXXXXXXXDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2977
            KSVINN+  LPNRKKSILKENQ VQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ
Sbjct: 1486 KSVINNS--LPNRKKSILKENQTVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2183          

HSP 2 Score: 665.226 bits (1715), Expect = 0.000e+0
Identity = 337/349 (96.56%), Postives = 341/349 (97.71%), Query Frame = 2
Query:    2 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEV 1048
            NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSE QISPNQ I   +  +I+N +
Sbjct:  910 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSELQISPNQLISLVNKSVINNSL 1258          
BLAST of SMED30011316 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1)

HSP 1 Score: 1396.33 bits (3613), Expect = 0.000e+0
Identity = 696/700 (99.43%), Postives = 697/700 (99.57%), Query Frame = 2
Query:  878 KSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFXXXXXXXXXXXXDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2977
            KSVINN+  LPNRKKSILKENQ VQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ
Sbjct: 1481 KSVINNS--LPNRKKSILKENQTVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2178          

HSP 2 Score: 665.226 bits (1715), Expect = 0.000e+0
Identity = 337/349 (96.56%), Postives = 341/349 (97.71%), Query Frame = 2
Query:    2 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEV 1048
            NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSE QISPNQ I   +  +I+N +
Sbjct:  905 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSELQISPNQLISLVNKSVINNSL 1253          
BLAST of SMED30011316 vs. Planmine SMEST
Match: SMESG000065696.1 (SMESG000065696.1)

HSP 1 Score: 1395.95 bits (3612), Expect = 0.000e+0
Identity = 696/700 (99.43%), Postives = 697/700 (99.57%), Query Frame = 2
Query:  878 KSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFXXXXXXXXXXXXDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2977
            KSVINN+  LPNRKKSILKENQ VQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ
Sbjct: 1482 KSVINNS--LPNRKKSILKENQTVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVGESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEFRNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPMENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKLHDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETADMDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQAISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVSYFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVDSENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDENVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTKVVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIIINQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ 2179          

HSP 2 Score: 664.84 bits (1714), Expect = 0.000e+0
Identity = 337/349 (96.56%), Postives = 341/349 (97.71%), Query Frame = 2
Query:    2 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEV 1048
            NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSE QISPNQ I   +  +I+N +
Sbjct:  906 NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMINSSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQRPMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIGCEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYHSIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFSSKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNSLLPNRKKSILKENQMVQDNLMTSSLNSELQISPNQLISLVNKSVINNSL 1254          
BLAST of SMED30011316 vs. Planmine SMEST
Match: SMESG000054674.1 (SMESG000054674.1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.272e-8
Identity = 42/132 (31.82%), Postives = 64/132 (48.48%), Query Frame = 2
Query: 2369 NFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVS-RRRKAKNHQDNEHKFQNYLRPLDGISIL-----TNESIRTKVVFFSKEICFEGAIK 2746
            +FID   + ++    N + SF S  DPI+   G+  RG GKR   K +S P E II   D    R + PSP+T+  R+ + +     +     +LRPLD  +I      +++  + KVVF   E  + G  K
Sbjct:  950 DFIDGFSTFVEKNDFNQSLSFVSPTDPILTTDGINFRGLGKRTRAKTVSTPYESIIYDGDQIQHRKIYPSPNTIKIRKARLRERMKTKSSLSKFLRPLDNETITICNPQSDQPEKIKVVFEKTEYSYFGFSK 1081          
BLAST of SMED30011316 vs. Planmine SMEST
Match: SMESG000054674.1 (SMESG000054674.1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.581e-8
Identity = 42/132 (31.82%), Postives = 64/132 (48.48%), Query Frame = 2
Query: 2369 NFIDFSLSVIKPVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDGNVIRMMPPSPDTVS-RRRKAKNHQDNEHKFQNYLRPLDGISIL-----TNESIRTKVVFFSKEICFEGAIK 2746
            +FID   + ++    N + SF S  DPI+   G+  RG GKR   K +S P E II   D    R + PSP+T+  R+ + +     +     +LRPLD  +I      +++  + KVVF   E  + G  K
Sbjct:  974 DFIDGFSTFVEKNDFNQSLSFVSPTDPILTTDGINFRGLGKRTRAKTVSTPYESIIYDGDQIQHRKIYPSPNTIKIRKARLRERMKTKSSLSKFLRPLDNETITICNPQSDQPEKIKVVFEKTEYSYFGFSK 1105          
The following BLAST results are available for this feature:
BLAST of SMED30011316 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011316 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000065696.10.000e+099.43SMESG000065696.1[more]
SMESG000065696.10.000e+099.43SMESG000065696.1[more]
SMESG000065696.10.000e+099.43SMESG000065696.1[more]
SMESG000054674.11.272e-831.82SMESG000054674.1[more]
SMESG000054674.11.581e-831.82SMESG000054674.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011316 ID=SMED30011316|Name=SMED30011316|organism=Schmidtea mediterranea sexual|type=transcript|length=3161bp
AAATCATAATATTGCCAATAAATCGATTATCAATGAATATCCAGAAATGA
ATTTGAATGAGAATGATAATTCACATCTACTAATCAATACTGATTCTGAA
AGATTTTCAAAAATTCCAGAAATGTCTTCGAAATCATTCCCGATGATAAA
TTCATCTAGCAGATATATCGATTCCTCAAGGCAATTATTGATGAGCAATT
CATCAAAAAAGTTCAATAGAGAGGATTTTTCTATTGAATTTAATGAAAAT
TTAAATCAATCGAATGTTAGTAACTCACCAATTGTTAATGTATTGCAACG
GCCAATGGAATGCTTAGAATCCTGTAAATTGTCCAATCCAAACGCGTCAG
GGCTAAAACATCAAAATAATTCCAATAAATCGATATTAAATAATAAAGGC
GGTGAATCACCTCGACATAACGTTATGATTGGTGCTAACGAATCTATAGG
TTGTGAACAACGTTTTTCCGACACAAATTCATTTAGAGAAATTGAACTTC
CGCAAAATTGTGTTTTGACCAATAATTCTTTAAATATGTCGCAAACCAAT
GAAAATGTCAGAATGGAATGCTTAGAAAAAAGTGAAAATGAATTGTATCA
CTCAATTGTTGATGAAAATAATTCTATTGAGCCTGAAATTCCATCTACAT
TAAATAATGAAAATAACTTATTGATGCCTCTGTCAATAAGTAAATCACCG
AATAATGTAATTATTATTGGAAATTCAGCACTGAAAAATTCGACTTTTTC
ATCAAAAATTTCGCCTCAAGAACAAACTGTTGAAAAATCATTTTCTAATC
GATTTTATAGAAAAGAAACTGGAACCTGTTCAAAAGTATATGATGATATA
GAAATGTCAAATGTTTTAGATAATCAAAAATCTGTAATCAATAATAATTC
ATTATTGCCAAATCGAAAAAAATCGATTTTAAAAGAAAATCAAATGGTAC
AAGATAATTTAATGACGTCCAGTCTCAATTCTGAATCGCAAATATCACCA
AATCAATCAATTGAACCGAATCACACACAAATTATCGATAATGAAGTTGG
GGAATCAGTAAATGCTGAGAAGAACGTGACTCCGACTGGGAAAACTGACA
AAGTTGACATTTGTTTAGAAAGAGAAGTTAATTCTAACGTTGATAAAATA
GAAATTAGTGCAAAACATATTGAAAATATTCCTGCCAGTGAAATCGAATT
TAGAAATAGTTTATGTGAAGAAATAAAGGATGGCAAAGAAACAGCGAGAA
ACCCAATACAGACCGATAACAACATTAGCAATGGGATTATAAATGAACCG
AATAAGGATCTGTTAGATGTAGAAACTGTAAATGAAAATTCAAAGCCGAT
GGAAAATATTATGGACAAAATTCACTCATCAACCGATATGAGTTTCCTGA
AACATTTGGCCGTACAAAGCCTGTCGAGAATAATATCACTGGAAACTGGT
GAAACCGATATCCAGCATTCACCGTATATTGATTTAGTAGAGGAAAAACT
GCATGATATTTTGTCGACGATCGTCACTAGTAGACAAAACGATATTGAGA
GTAGGAAAGGTAAAAATAACGGAACTCAAACAGATAAAATAGTTAGAAAG
AGATCTGTAAAAATCCAATGCGAATTATCTGATTACGAAGAAACAGCTGA
TATGGATTTGAAAACGATTTGGATTAGGAAAGCTATGGATTTCAAAGGCG
ATGAAATGAAATCATCGATAAAAGATGTTGAAGGTGAAATAATACCGAGA
GAAACCAAAGTTCCGTTGGTTTGCGATAATGACGTGGAATCATCACAAGC
CATTTCTAGAATTAATTTACCACCGAGCCATTCCAAAATTGCAAAATCTC
AACAACCGGGAAACGATATAGAAATTCCGAATATAATCGATTCCGATAAA
CACCTGTCAATAAGGAATACTAATGAATTGGAATCCAATTTCAAGGTCAG
TTACTTCGAATCGACTCGAGTGATGGGAGGAAATAATCAGAAATTGCAAG
TGAACCTTTCATCAATCGATAATGTAGCGACATCATCAGCACAAACCACC
GATAATCCCACTAATGGAAACTTCTCCTCGGAATATAAATCAATAGTTGA
TTCGGAAAATGAAAATATAGATTCATTTTCAGGAGGAGGAAAACTTCCAA
GTGCTGTTAAGCACTTACCACATGATAAAAATCAATATTCCGTTATCCCA
GAAGAAGTTGATGAGATTTCAGGAATGAAACAATCCGATAATGTAGATGA
AAATGTCTCCATTCCGACTGATAAACAATCGATTAATCCCGTTGTAAAAC
GCACCCGGAATTATAAAATCGATTTAAATGATATTTCATCAATAAAACGA
GACCCAGGAAATCCATTTAACTTTATTGATTTCTCGCTGTCAGTCATCAA
GCCGGTATTGCGCAATACTGCCAGGAGTTTCCAGAGCGTGAATGATCCGA
TCATACTTGATCCCGGAGTGATTGACAGGGGACACGGGAAACGCAAGGTC
ATAAAGCCGATATCCCATCCCAAGGAGGTAATCATCAGGAGGAAGGATGG
CAATGTCATCAGGATGATGCCGCCGTCACCGGACACCGTGAGCAGGAGGA
GGAAGGCCAAGAACCATCAAGATAACGAGCATAAGTTTCAGAACTATTTG
CGGCCCCTCGATGGAATTAGTATTTTAACCAATGAATCAATAAGAACAAA
AGTTGTTTTCTTTTCTAAAGAGATTTGTTTTGAAGGGGCAATAAAATCCA
AACCGACCAAAATCCAGTTTAGGACGCTTACTAGAACTAAAACCAACACA
AAAACCGATGCAATGTTTCGGTGCCCATTTAAAAATGTCTACATCATAAT
AAATCAGTATAAGTTTCTATTCGAACCGGATAAGATTTTTTTAGTCCCGA
AAGCTGTTCCTTTTATGTTTTATAATCGAAGCGAATTGGCGGTCACAATC
ACTGAAACTTGTCCTTCTTTGCCACAGTAAAAATCAGCATCAGTCGTCTT
TCAGATTATTTTCACTAATTTATAATGTTTTATTGATTTTCACAAAAGTT
TGTTTATTGATTTTCAAATTTAAAAAAGCGCGAGTGCCATGCGATAAAAA
TATGTAGGTATATAGCCGACGGAGCAAGAACCGAGGAGAGGATTCCAGTT
TCGGATTGTCT
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protein sequence of SMED30011316-orf-1

>SMED30011316-orf-1 ID=SMED30011316-orf-1|Name=SMED30011316-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=993bp
NHNIANKSIINEYPEMNLNENDNSHLLINTDSERFSKIPEMSSKSFPMIN
SSSRYIDSSRQLLMSNSSKKFNREDFSIEFNENLNQSNVSNSPIVNVLQR
PMECLESCKLSNPNASGLKHQNNSNKSILNNKGGESPRHNVMIGANESIG
CEQRFSDTNSFREIELPQNCVLTNNSLNMSQTNENVRMECLEKSENELYH
SIVDENNSIEPEIPSTLNNENNLLMPLSISKSPNNVIIIGNSALKNSTFS
SKISPQEQTVEKSFSNRFYRKETGTCSKVYDDIEMSNVLDNQKSVINNNS
LLPNRKKSILKENQMVQDNLMTSSLNSESQISPNQSIEPNHTQIIDNEVG
ESVNAEKNVTPTGKTDKVDICLEREVNSNVDKIEISAKHIENIPASEIEF
RNSLCEEIKDGKETARNPIQTDNNISNGIINEPNKDLLDVETVNENSKPM
ENIMDKIHSSTDMSFLKHLAVQSLSRIISLETGETDIQHSPYIDLVEEKL
HDILSTIVTSRQNDIESRKGKNNGTQTDKIVRKRSVKIQCELSDYEETAD
MDLKTIWIRKAMDFKGDEMKSSIKDVEGEIIPRETKVPLVCDNDVESSQA
ISRINLPPSHSKIAKSQQPGNDIEIPNIIDSDKHLSIRNTNELESNFKVS
YFESTRVMGGNNQKLQVNLSSIDNVATSSAQTTDNPTNGNFSSEYKSIVD
SENENIDSFSGGGKLPSAVKHLPHDKNQYSVIPEEVDEISGMKQSDNVDE
NVSIPTDKQSINPVVKRTRNYKIDLNDISSIKRDPGNPFNFIDFSLSVIK
PVLRNTARSFQSVNDPIILDPGVIDRGHGKRKVIKPISHPKEVIIRRKDG
NVIRMMPPSPDTVSRRRKAKNHQDNEHKFQNYLRPLDGISILTNESIRTK
VVFFSKEICFEGAIKSKPTKIQFRTLTRTKTNTKTDAMFRCPFKNVYIII
NQYKFLFEPDKIFLVPKAVPFMFYNRSELAVTITETCPSLPQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000429neoblast
PLANA:0002109X1 cell