Acidic phospholipase A2

Overview
NameAcidic phospholipase A2
Smed IDSMED30011291
Length (bp)598
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Acidic phospholipase A2 (SMED30011291) t-SNE clustered cells

Violin plots show distribution of expression levels for Acidic phospholipase A2 (SMED30011291) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Acidic phospholipase A2 (SMED30011291) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Acidic phospholipase A2 (SMED30011291) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011291

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30011291SMESG000079712.1 dd_Smed_v4_2969_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011291SMESG000079712.1 dd_Smed_v4_2969_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011291SMESG000079712.1 Contig45991newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011291SMESG000079712.1 Contig45991uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011291SMESG000079713.1 SMESG000079712.1 Contig10980newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011291SMESG000079713.1 SMESG000079712.1 Contig10980uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30011291SMESG000079712.1 dd_Smed_v6_2969_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
gutSMED30011291SMESG000079712.1 SMED30011291smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30011291SMESG000079712.1 SMED30011291smed_20140614PMID:26457503
Tu et al., 2015		whole organism asexual adult colorimetric in situ hybridization evidence
Category 3 cellSMED30011291SMESG000079712.1 dd_Smed_v6_2969_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30011291SMESG000079712.1 dd_Smed_v4_2969_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Match: PLA2G5 (phospholipase A2 group V [Source:HGNC Symbol;Acc:HGNC:9038])

HSP 1 Score: 62.7734 bits (151), Expect = 3.134e-12
Identity = 37/109 (33.94%), Postives = 56/109 (51.38%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            ++  +M++ V  +N     G YG YCG G   G P D  D CC  HD CY   E     N    SY + +  GVV+C+        LC CD+ + YC++ N +++NP++
Sbjct:   23 LDLKSMIEKVTGKNALTNYGFYGCYCGWGG-RGTPKDGTDWCCWAHDHCYGRLEEK-GCNIRTQSYKYRFAWGVVTCEPGPFCHVNLCACDRKLVYCLKRNLRSYNPQY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 60.4622 bits (145), Expect = 2.492e-11
Identity = 34/109 (31.19%), Postives = 52/109 (47.71%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            + F+ M+K    +  +L  G YG +CG G   G P D  D CC  HD CY+  E        L SY +S +   ++C +    R +LC+CD+    C   N   +N ++
Sbjct:   23 VNFHRMIKLTTGKEAALSYGFYGCHCGVGG-RGSPKDATDRCCVTHDCCYKRLEKRGCGTKFL-SYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 60.4622 bits (145), Expect = 2.492e-11
Identity = 34/109 (31.19%), Postives = 52/109 (47.71%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            + F+ M+K    +  +L  G YG +CG G   G P D  D CC  HD CY+  E        L SY +S +   ++C +    R +LC+CD+    C   N   +N ++
Sbjct:   23 VNFHRMIKLTTGKEAALSYGFYGCHCGVGG-RGSPKDATDRCCVTHDCCYKRLEKRGCGTKFL-SYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Match: PLA2G2A (phospholipase A2 group IIA [Source:HGNC Symbol;Acc:HGNC:9031])

HSP 1 Score: 56.225 bits (134), Expect = 5.446e-10
Identity = 33/104 (31.73%), Postives = 49/104 (47.12%), Query Frame = 2 
Query:  110 MLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            M+K    +  +L  G YG +CG G   G P D  D CC  HD CY+  E        L SY +S +   ++C +    R +LC+CD+    C   N   +N ++
Sbjct:    1 MIKLTTGKEAALSYGFYGCHCGVGG-RGSPKDATDRCCVTHDCCYKRLEKRGCGTKFL-SYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKY 102
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Match: PLA2G1B (phospholipase A2 group IB [Source:HGNC Symbol;Acc:HGNC:9030])

HSP 1 Score: 56.6102 bits (135), Expect = 8.018e-10
Identity = 33/104 (31.73%), Postives = 52/104 (50.00%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRNPSLLV--GYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHK-----NNPLLTSYSWSYNEGVVSCDQNTKPRQK-LCKCDQGITYCV 385
            +F  M+K V+  +   L    YG YCG G   G P+DE+D CC+ HD+CY+  +         +NP   +YS+S +   ++C    K  +  +C CD+    C 
Sbjct:   26 QFRKMIKCVIPGSDPFLEYNNYGCYCGLGG-SGTPVDELDKCCQTHDNCYDQAKKLDSCKFLLDNPYTHTYSYSCSGSAITCSSKNKECEAFICNCDRNAAICF 128
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Celegans
Match: Y69A2AL.2 (Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q3V5J7])

HSP 1 Score: 51.9878 bits (123), Expect = 1.410e-8
Identity = 30/81 (37.04%), Postives = 42/81 (51.85%), Query Frame = 2 
Query:  155 YGRYCGKGNLPGKPIDEIDSCCKVHDDCYE----TWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCV 385
            YG  C  G  P KPID ID CC+VH+DCY     T +  + N+P    Y W       +C+  +K  Q +C+CD+    C+
Sbjct:   41 YGCSCS-GISPNKPIDGIDRCCQVHNDCYNELLLTKKCQNSNSPYFCLYKWECVYQQPACNNESKCTQSVCQCDEQFINCL 120
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Celegans
Match: C07E3.9 (Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q17790])

HSP 1 Score: 44.669 bits (104), Expect = 7.027e-6
Identity = 27/81 (33.33%), Postives = 40/81 (49.38%), Query Frame = 2 
Query:  155 YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPL----LTSYSWS-YNEGVVSCDQNTKPRQKLCKCDQGITYC 382
            YG +CG G    +P+D ID CC  HD CY+   ++     +    +  YSWS  N   +  D+N   +  LC CD+ +  C
Sbjct:   47 YGCWCGIGG-SHEPVDGIDECCMHHDKCYDAAVDNKVCMDVEIEYVDDYSWSCMNSTAICSDKNMGCKAALCDCDKIVVEC 126
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Zebrafish
Match: pla2g1b (phospholipase A2, group IB (pancreas) [Source:ZFIN;Acc:ZDB-GENE-070117-1])

HSP 1 Score: 58.151 bits (139), Expect = 1.148e-10
Identity = 40/118 (33.90%), Postives = 65/118 (55.08%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRN-PSL-LVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCY-ETWENSH----KNNPLLTSYSWSYNE--GVVSC--DQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +F  M+   +  + P+L    YG YCGKG   G P+DE+D CC+VHD CY ++W++       +NP    YS++ ++   +VSC  D+N      +C+CD+   +C       +NPE+
Sbjct:   28 QFRAMILCTIPDSWPALDYADYGCYCGKGG-SGTPVDELDRCCQVHDQCYSDSWQHDDCWGILDNPYTEIYSFACDKPAKIVSCNADKNRPCEMFICECDRIAAHCFAQ--AGYNPEY 142
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Zebrafish
Match: zgc:92162 (zgc:92162 [Source:ZFIN;Acc:ZDB-GENE-040718-41])

HSP 1 Score: 49.2914 bits (116), Expect = 2.511e-7
Identity = 35/109 (32.11%), Postives = 50/109 (45.87%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQ-NTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +E   ++K    R+    V YG YCG G   G P D  D CC  HD CY   E +         Y W+ ++    CD  N +  + LC+CD+    C+R     FNP++
Sbjct:   31 LELAGVIKCSTGRSALSYVMYGCYCGLGG-QGWPRDRADWCCHKHDCCYGDAEFAGCQTK-TDRYHWTCDDEQADCDSLNDRCAKILCRCDREAARCLRK--APFNPKY 135
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Match: cntln (centlein [Source:Xenbase;Acc:XB-GENE-953151])

HSP 1 Score: 55.4546 bits (132), Expect = 1.707e-9
Identity = 34/105 (32.38%), Postives = 56/105 (53.33%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRNPSLLV--GYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHK-----NNPLLTSYSWSYNE-GVVSCDQ--NTKPRQKLCKCDQGITYC 382
            +F+N++K  +  +  LL    YG YCG G   G P+DE+D CC++HD+CY+     +       NP   +YS++  +  ++SC+   N +    +CKCD     C
Sbjct:   27 QFSNIIKCAIPGSQPLLEFNDYGCYCGLGG-SGTPVDELDRCCQIHDNCYDKASLINNCVPVLQNPYTETYSYTCTDNNMMSCNSKYNNECDMHICKCDMNAALC 130
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Match: sgtb (small glutamine-rich tetratricopeptide repeat (TPR)-containing, beta [Source:Xenbase;Acc:XB-GENE-1011551])

HSP 1 Score: 51.9878 bits (123), Expect = 2.945e-8
Identity = 32/113 (28.32%), Postives = 56/113 (49.56%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRNPSLLV--GYGRYCGKGNLPGKPIDEIDSCCKVHDDCY---ETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQK-LCKCDQGITYCVRDNFKAFNPEH 418
            +F N++K V+  +        YG YCG G   G P+D +D CC+ HD CY   + + N   ++P +  Y+++ +   V+C  +    +K +C CD+    C       +NP +
Sbjct:   24 QFRNIIKCVIPSSDPYFDYNDYGCYCGIGG-SGTPVDALDRCCQTHDKCYSDSKGYCNGILDSPYIELYAYTCSGTSVTCSSSNNACEKFICDCDKNAAICFSR--AGYNPAY 133
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 50.8322 bits (120), Expect = 7.551e-8
Identity = 36/109 (33.03%), Postives = 48/109 (44.04%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKL-CKCDQGITYCVRDNFKAFNPEH 418
            I+    ++    R+    +GYG +CG G   G P D  D CC  HD CYE  E  +     L  YSW+  +  V C       QK+ CKCD     C+      FN +H
Sbjct:   18 IQLAGTIQCGTGRSAVHYIGYGCHCGLGG-QGVPKDNTDWCCHSHDCCYEFAEK-YGCKTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKH 122
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 50.8322 bits (120), Expect = 1.069e-7
Identity = 36/109 (33.03%), Postives = 48/109 (44.04%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKL-CKCDQGITYCVRDNFKAFNPEH 418
            I+    ++    R+    +GYG +CG G   G P D  D CC  HD CYE  E  +     L  YSW+  +  V C       QK+ CKCD     C+      FN +H
Sbjct:   24 IQLAGTIQCGTGRSAVHYIGYGCHCGLGG-QGVPKDNTDWCCHSHDCCYEFAEK-YGCKTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKH 128
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Match: eml6 (echinoderm microtubule associated protein like 6 [Source:Xenbase;Acc:XB-GENE-6039541])

HSP 1 Score: 50.8322 bits (120), Expect = 1.143e-7
Identity = 35/109 (32.11%), Postives = 49/109 (44.95%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            I+    ++    R+    +GYG +CG G   G P D  D CC  HD CYE  E  +     L  YSW+  +  V C D     ++ +CKCD     C+      FN +H
Sbjct:   34 IQLAGTIQCGTGRSAVHYIGYGCHCGLGG-QGVPKDNTDWCCHSHDCCYEFAEK-YGCKTKLGQYSWTCKDNTVKCGDMKDWCQKIVCKCDSKFAECLSKT--RFNSKH 138
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 67.0106 bits (162), Expect = 5.233e-14
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLT-SYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +E  +M++ V ++N     G YG YCG G   G P D  D CC++HD CY   E   K+  + T SY + Y  G+V C+ ++    +LC CD+ + YC+R N   +NP +
Sbjct:   23 LELKSMIEKVTRKNAFKNYGFYGCYCGWGG-RGTPKDGTDWCCQMHDRCYGQLE--EKDCAIRTQSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 67.0106 bits (162), Expect = 5.233e-14
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLT-SYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +E  +M++ V ++N     G YG YCG G   G P D  D CC++HD CY   E   K+  + T SY + Y  G+V C+ ++    +LC CD+ + YC+R N   +NP +
Sbjct:   23 LELKSMIEKVTRKNAFKNYGFYGCYCGWGG-RGTPKDGTDWCCQMHDRCYGQLE--EKDCAIRTQSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 67.0106 bits (162), Expect = 5.233e-14
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLT-SYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +E  +M++ V ++N     G YG YCG G   G P D  D CC++HD CY   E   K+  + T SY + Y  G+V C+ ++    +LC CD+ + YC+R N   +NP +
Sbjct:   23 LELKSMIEKVTRKNAFKNYGFYGCYCGWGG-RGTPKDGTDWCCQMHDRCYGQLE--EKDCAIRTQSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Match: Pla2g5 (phospholipase A2, group V [Source:MGI Symbol;Acc:MGI:101899])

HSP 1 Score: 67.0106 bits (162), Expect = 5.233e-14
Identity = 40/110 (36.36%), Postives = 61/110 (55.45%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVG-YGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLT-SYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +E  +M++ V ++N     G YG YCG G   G P D  D CC++HD CY   E   K+  + T SY + Y  G+V C+ ++    +LC CD+ + YC+R N   +NP +
Sbjct:   23 LELKSMIEKVTRKNAFKNYGFYGCYCGWGG-RGTPKDGTDWCCQMHDRCYGQLE--EKDCAIRTQSYDYRYTNGLVICEHDSFCPMRLCACDRKLVYCLRRNLWTYNPLY 129
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Match: Pla2g10 (phospholipase A2, group X [Source:MGI Symbol;Acc:MGI:1347522])

HSP 1 Score: 56.225 bits (134), Expect = 7.133e-10
Identity = 34/98 (34.69%), Postives = 50/98 (51.02%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCD-QNTKPRQKLCKCDQGITYCV 385
            +E    L  V  R+P   + YG YCG G   G+P D ID CC  HD CY   +++   +P L  Y W   +  + C     K ++ LC+CD+ + YC+
Sbjct:   31 LELAGTLDCVGPRSPMAYMNYGCYCGLGGH-GEPRDAIDWCCYHHDCCYSRAQDA-GCSPKLDRYPWKCMDHHILCGPAENKCQELLCRCDEELAYCL 126
[Close]
BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Match: sp|P0DMT3|PA2HS_ECHCO (Phospholipase A2 homolog ECO_00035 OS=Echis coloratus OX=64175 PE=1 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.567e-16
Identity = 35/108 (32.41%), Postives = 61/108 (56.48%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            IE   M+  +  + P+  V YG +CG G+  GKP D  D CC VH  CY+T  +    +P    Y + +  G + C+ +T  ++++C+CD+ +  C+R+N K +N ++
Sbjct:    3 IELGKMIVQLTNKTPASYVSYGCFCGGGDR-GKPKDATDRCCFVHSCCYDTLPDC---SPKTDQYKYKWENGEIICENSTSCKKRICECDKAVAICLRENLKTYNKKY 106
[Close]
BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Match: sp|Q8AXW7|PA2B_MICCO (Basic phospholipase A2 OS=Micrurus corallinus OX=54390 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 2.145e-16
Identity = 41/99 (41.41%), Postives = 54/99 (54.55%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSY--SWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            I F  M++   +R+      YG YCG G   G P+DE+D CCKVHDDCY   E  H+ +P LT Y  + S   G V+C D  TK +  +C CD+    C
Sbjct:   30 INFQRMIQCTTRRSAWDFTNYGCYCGAGG-SGTPVDELDRCCKVHDDCYGAAEKYHRCSPKLTLYTSTCSSQTGSVTCKDNGTKCKAFVCNCDRTAALC 127
[Close]
BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Match: sp|P0DMT1|PA2HS_ECHPL (Phospholipase A2 homolog EPL_00195 OS=Echis pyramidum leakeyi OX=38415 PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.608e-16
Identity = 35/108 (32.41%), Postives = 60/108 (55.56%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            IE   M+  +  + P+  V YG +CG G+  GKP D  D CC VH  CY+T  +    +P    Y + +  G + C+ +T  ++++C+CD+ +  C+RDN   +N ++
Sbjct:    3 IELGKMIIQLTNKTPASYVSYGCFCGGGD-KGKPKDATDRCCFVHSCCYDTLPDC---SPKTDQYKYKWENGEIICENSTSCKKRICECDKAVAICLRDNLNTYNKKY 106
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BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Match: sp|Q71QE8|PA2BN_CROVV (Basic phospholipase A2 Cvv-N6 OS=Crotalus viridis viridis OX=8742 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 9.322e-16
Identity = 39/109 (35.78%), Postives = 58/109 (53.21%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNP-SLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            ++FN M+K + K+N       YG YCG G   G+P D  D CC VHD CYE   N    +P    YS+S+  GV+ C + T   +++C+CD+    C R+N   +   +
Sbjct:   19 LQFNKMIKMMTKKNAFPFYTSYGCYCGWGG-RGRPKDATDRCCFVHDCCYEKLTNC---SPKTDIYSYSWKRGVIICGKGTPCEKQICECDRAAAVCFRENLPTYKKRY 123
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BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Match: sp|P0DPT7|PA21_SCODE (Phospholipase A2 SSD387 OS=Scolopendra dehaani OX=2609776 PE=1 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.014e-15
Identity = 44/116 (37.93%), Postives = 60/116 (51.72%), Query Frame = 2 
Query:  101 FNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENS----HKNNPLLTSYSWSYNEGVVSCD-QNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQG 433
            F +M     KR+P    GYG YCG G   G P+D ID CC+VHD+CY T   +    +  N  L  Y W      + CD ++    + LCKCD+ I  C  +N K +NPE+ +  G
Sbjct:   33 FLSMTLTAAKRSPQEYDGYGNYCGWGG-EGTPVDSIDRCCQVHDNCYGTVNENECGHYIRNVKLIDYDWHMEGTEIVCDPKDDACGRALCKCDKDIIDCFNENDKDYNPEYNKVIG 147
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Match: U3F588 (Phospholipase A2 22 OS=Micrurus fulvius OX=8637 PE=2 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 7.225e-15
Identity = 41/97 (42.27%), Postives = 55/97 (56.70%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            I F  M++   +R+      YG YCG G   G P+DE+D CCKVHDDCY   E  HK +P  T YS+  + G ++C D NTK +  +C CD+    C
Sbjct:   30 INFKRMIECTTRRSVWDFTNYGCYCGSGGR-GTPVDELDRCCKVHDDCYAAAEKYHKCSPKWTLYSYDCSNGQLTCKDNNTKCKDFVCNCDRTAALC 125
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Match: A0A194ARB4 (Phospholipase A2 6e OS=Micrurus tener OX=1114301 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 9.026e-15
Identity = 41/97 (42.27%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            ++F NM++   KR+      YG YCG G   G P+DE+D CC+VHDDCY   E  H   P LT YS   +EG ++C D +TK +  +C CD+    C
Sbjct:   30 LDFKNMIECTTKRSVWDFTNYGCYCGAGG-SGTPVDELDRCCQVHDDCYGEAEKVHGCWPKLTLYSHECSEGQLTCKDNDTKCQDFVCNCDRAAALC 125
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Match: A0A194AT57 (Phospholipase A2 6f OS=Micrurus tener OX=1114301 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.740e-14
Identity = 41/97 (42.27%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            ++F NM++   KR+      YG YCG G   G P+DE+D CC+VHDDCY   E  H   P LT YS   +EG ++C D +TK +  +C CD+    C
Sbjct:   30 LDFKNMIECTTKRSVWDFTNYGCYCGAGG-SGTPVDELDRCCQVHDDCYGEAEKVHGCWPKLTLYSHECSEGQLTCKDNDTKCQDFVCNCDRTAALC 125
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Match: A0A194AT61 (Phospholipase A2 6a OS=Micrurus tener OX=1114301 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.894e-14
Identity = 41/97 (42.27%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            ++F NM++   KR+      YG YCG G   G P+DE+D CC+VHDDCY   E  H   P LT YS   +EG ++C D +TK +  +C CD+    C
Sbjct:   30 LDFKNMIECTTKRSVWDFTNYGCYCGAGG-SGTPVDELDRCCQVHDDCYGEAEKVHGCWPKLTLYSHECSEGQLTCKDNDTKCQDFVCNCDRTAALC 125
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Match: A0A194AS73 (Phospholipase A2 6d OS=Micrurus tener OX=1114301 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.914e-14
Identity = 41/97 (42.27%), Postives = 56/97 (57.73%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC-DQNTKPRQKLCKCDQGITYC 382
            ++F NM++   KR+      YG YCG G   G P+DE+D CC+VHDDCY   E  H   P LT YS   +EG ++C D +TK +  +C CD+    C
Sbjct:   30 LDFKNMIECTTKRSVWDFTNYGCYCGAGG-SGTPVDELDRCCQVHDDCYGEAEKVHGCWPKLTLYSHECSEGQLTCKDNDTKCQDFVCNCDRTAALC 125
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BLAST of Acidic phospholipase A2 vs. Ensembl Cavefish
Match: ENSAMXT00000030049.1 (phospholipase A2-like [Source:NCBI gene;Acc:103028136])

HSP 1 Score: 52.373 bits (124), Expect = 1.187e-8
Identity = 34/112 (30.36%), Postives = 53/112 (47.32%), Query Frame = 2 
Query:   98 EFNNMLKWVLK-RNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKN---NPLLTSYSWSYNEGVVSCD-QNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +F NM++      NP +   YG +CG G   G P D++D CC+VHD CY   +   +    +P +  YS S +    SC   N K    +C+CD+   +C       +N  +
Sbjct:   15 QFGNMIQCAQPGVNPLIYNNYGCFCGLGG-SGSPKDQLDRCCQVHDQCYTNAKKLPECTFLDPYVKVYSHSCSNKKASCSGSNAKCEAAVCECDRVAAHCFAQYNHVYNSAY 125
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BLAST of Acidic phospholipase A2 vs. Ensembl Cavefish
Match: ENSAMXT00000031293.1 (phospholipase A2-like [Source:NCBI gene;Acc:103028136])

HSP 1 Score: 51.9878 bits (123), Expect = 1.883e-8
Identity = 34/112 (30.36%), Postives = 53/112 (47.32%), Query Frame = 2 
Query:   98 EFNNMLKWVLK-RNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKN---NPLLTSYSWSYNEGVVSCD-QNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +F NM++      NP +   YG +CG G   G P D++D CC+VHD CY   +   +    +P +  YS S +    SC   N K    +C+CD+   +C       +N  +
Sbjct:   26 QFGNMIQCAQPGVNPLIYNNYGCFCGLGG-SGSPKDQLDRCCQVHDQCYTNAKKLPECTFLDPYVKVYSHSCSNKKASCSGSNAKCEAAVCECDRVAAHCFAQYNHVYNSAY 136
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BLAST of Acidic phospholipase A2 vs. Ensembl Cavefish
Match: ENSAMXT00000000319.2 (phospholipase A2 [Source:NCBI gene;Acc:103024514])

HSP 1 Score: 48.1358 bits (113), Expect = 6.472e-7
Identity = 35/117 (29.91%), Postives = 56/117 (47.86%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRN-PSL-LVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHK-----NNPLLTSYSWS---YNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            +F +M+   +  + P+L    YG YCGKG   G P+D++D CC+VHD CY      +      +NP    Y++S    N+ +     N +    +C+CD+    C       +N EH
Sbjct:   39 QFRDMIICTIPDSWPALDYSDYGCYCGKGG-SGTPVDDLDRCCEVHDRCYSDAMQHNACWPILDNPYTEIYAYSCDKENKKITCKSNNNECEMFICECDRKAAECFA--VSDYNEEH 152
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BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008226.1 (pep scaffold:Pmarinus_7.0:GL476891:263168:265744:1 gene:ENSPMAG00000007428.1 transcript:ENSPMAT00000008226.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 59.6918 bits (143), Expect = 6.071e-12
Identity = 33/97 (34.02%), Postives = 52/97 (53.61%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCD-QNTKPRQKLCKCDQGITYC 382
            ++F NM++  + R P    GYG +CG G   G P+D  D CC+ HD CY     +   NP    Y ++   G ++CD +N    +K+C+CD+  + C
Sbjct:   36 VQFGNMIECRMNRQPLDYYGYGCHCGFGG-KGAPVDPTDRCCRSHDCCYNRAITA-GCNPYSDIYHYACKHGRITCDAKNDWCERKICECDRRASAC 130
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BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008209.1 (pep scaffold:Pmarinus_7.0:GL476891:251313:253691:-1 gene:ENSPMAG00000007418.1 transcript:ENSPMAT00000008209.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 57.3806 bits (137), Expect = 4.392e-11
Identity = 36/113 (31.86%), Postives = 56/113 (49.56%), Query Frame = 2 
Query:  101 FNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKL-CKCDQGITYCVRDNFKAFNPEHYRRQGI 436
            F  M+  V  RNP    GYG +C  G+  G P+D +D CC+VH+ CY+    S      L SY +S     ++C +   P + L C CD+    C   +    N ++++R G+
Sbjct:   33 FAWMIYRVTGRNPLDYSGYGCHCIMGS-EGHPVDAVDRCCQVHNRCYDA-AFSMGCASDLDSYEFSTRGKAITCRKQGSPCETLACSCDKAAVECFAIHDFDDNFQNFKRNGV 143
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008454.1 (pep scaffold:Pmarinus_7.0:GL483377:2289:3737:-1 gene:ENSPMAG00000007656.1 transcript:ENSPMAT00000008454.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.595 bits (109), Expect = 2.650e-7
Identity = 35/113 (30.97%), Postives = 51/113 (45.13%), Query Frame = 2 
Query:  101 FNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKP-RQKLCKCDQGITYC-VRDNF----KAFNPEHY 421
            F  M+  V+  NP+   GYG  C      GKP+D++D CC+ H  CYE   N    +P    Y +S     + C +   P     C+CD+    C  R +F    + FN + Y
Sbjct:    5 FAWMIWKVVGINPAEYSGYGCNCLSTGH-GKPVDDVDRCCQAHTKCYEQ-VNKMSCSPDWDPYYFSIRGKAIRCTRGGPPCDVHTCRCDKAAVECFARSHFNRRYRNFNRKRY 115
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BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008199.1 (pep scaffold:Pmarinus_7.0:GL476891:241341:242643:-1 gene:ENSPMAG00000007414.1 transcript:ENSPMAT00000008199.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 46.2098 bits (108), Expect = 5.219e-7
Identity = 31/110 (28.18%), Postives = 47/110 (42.73%), Query Frame = 2 
Query:  101 FNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQ 430
            F  M+  V++ NP+   GYG  C      GKP+D++D CC+ H  CYE        +P    Y +S     + C +   P      CD     C +   + F   H+ R+
Sbjct:   28 FAWMIWKVVRINPAEYSGYGCNCLSTG-HGKPVDDVDRCCQAHTKCYEQ-VYKMSCSPHWDPYYFSIRGKAIRCTRGGPP------CDVHTCLCDKAAVECFARSHFNRR 129
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BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008463.1 (pep scaffold:Pmarinus_7.0:GL483377:12502:14271:-1 gene:ENSPMAG00000007657.1 transcript:ENSPMAT00000008463.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 45.8246 bits (107), Expect = 6.688e-7
Identity = 34/115 (29.57%), Postives = 51/115 (44.35%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKP-RQKLCKCDQGITYC-VRDNF----KAFNPEHY 421
            + F  M+  V+  NP+   GYG  C      GKP+D++D CC+ H  CYE        +P    Y +S     + C +   P     C+CD+    C  R +F    + FN + Y
Sbjct:   21 LAFALMIWKVVGINPAEYSGYGCNCLSTG-HGKPVDDVDRCCQAHTKCYEQVYKM-SCSPHWDPYYFSIRGKAIRCTRGGPPCDVHTCRCDKAAVECFARSHFNRRYRNFNRKRY 133
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Match: EDO34714 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SNJ7])

HSP 1 Score: 57.3806 bits (137), Expect = 1.777e-10
Identity = 36/92 (39.13%), Postives = 45/92 (48.91%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCD--QNTKPRQKLCKCD 364
             +F+ M+K   KRNP     YG YCG G   G P+D +D CC VHD CY+    S             Y  G   CD  +N+K  Q LC+CD
Sbjct:   45 FDFHAMIKCETKRNPLDYNYYGCYCGFGG-RGSPVDGLDKCCYVHDMCYKASRTSGICWAGQAYIHLYYRTGCTGCDKAKNSKCGQMLCECD 135
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Match: EDO43504 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RYD8])

HSP 1 Score: 54.6842 bits (130), Expect = 3.263e-10
Identity = 36/111 (32.43%), Postives = 49/111 (44.14%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCD------QNTKPRQKLCKCDQGITYCVRDNFKAFNP 412
            +F  M+K    R      GYG +CG+G   G P+D +D CC VHD C   W+      P    YS+S ++   SC        N    Q +C CD+    C   N   +NP
Sbjct:    1 QFGRMVKCATGRRAWDYNGYGNWCGRGGR-GTPVDGVDRCCMVHDHC---WDRLVHCRPYSNKYSYSVSDRHPSCSITCNAGNNDSCEQSICNCDKAAAECFARN--TYNP 105
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Match: EDO38422 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCY6])

HSP 1 Score: 48.1358 bits (113), Expect = 1.261e-7
Identity = 21/57 (36.84%), Postives = 35/57 (61.40%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNP-SLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSH 262
             +  NM+K ++ + P +  V YG YCG G   G P+D++D CC+ HD CY+   +++
Sbjct:   41 FQLANMMKCLIPQRPWAKYVAYGCYCGPGG-EGIPVDDVDRCCQAHDTCYDKVSSNY 96
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Match: EDO40720 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6H4])

HSP 1 Score: 47.3654 bits (111), Expect = 2.461e-7
Identity = 33/119 (27.73%), Postives = 54/119 (45.38%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSY-NEGVVSCDQNTKPRQ-KLCKCDQGITYCVR-----DNFKAFNPEHYRRQ 430
            ++F  M+K    R+     GYG +CGKG   G+ +D  D CC +HD CY+           +  Y+  Y  +G     +   P Q ++C+CD     C +     + +K +N   Y R+
Sbjct:    7 VQFGLMIKCTTGRSAFDYNGYGCWCGKGG-SGRAVDATDRCCLIHDRCYDAVIRDRVCPWGINVYTTVYFRKGCNIFGKTNNPCQYRICQCDSAAVRCFKRSYYNNRYKDYNKNKYCRK 124
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Match: EDO40742 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7S6G1])

HSP 1 Score: 43.5134 bits (101), Expect = 6.914e-6
Identity = 32/114 (28.07%), Postives = 42/114 (36.84%), Query Frame = 2 
Query:  101 FNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSC---------DQNTKPRQKLCKCDQGITYCVRDNFKAFNPE 415
            F  M+      +    + YG +CG G   G P+DE D CC +HD CY+              Y   Y      C         D   + R  LCKCD     C +     FNP+
Sbjct:    6 FRKMIGCHTGNSAFDYIDYGCWCGLGG-NGSPLDETDECCYIHDKCYDDIIARSTCGYSFQVYFRDYKHSCTRCEPLSSYPSDDYYRECRVDLCKCDSEAAKCFKQ--ARFNPD 116
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Medaka
Match: ENSORLT00000032129.1 (phospholipase A2 group IB [Source:NCBI gene;Acc:101155667])

HSP 1 Score: 48.9062 bits (115), Expect = 3.090e-7
Identity = 32/98 (32.65%), Postives = 46/98 (46.94%), Query Frame = 2 
Query:  155 YGRYCGKGNLPGKPIDEIDSCCKVHDDCY-------ETWENSHKNNPLLTSYSWSYNEG---VVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEH 418
            YG YCG G   G P+DE+D CC+VHD CY       E W     +NP    Y ++ +E    +     N +    +C+CD+    C       ++PEH
Sbjct:   48 YGCYCGLGG-SGNPVDELDRCCQVHDQCYSDAMQHPECWPI--LDNPYTEIYDYTCDEANKKLTCTSSNDECEMFICECDRKAAECF--GISTWHPEH 140
[Close]
BLAST of Acidic phospholipase A2 vs. Ensembl Medaka
Match: zgc:92162 (phospholipase A2 [Source:NCBI gene;Acc:101170929])

HSP 1 Score: 45.4394 bits (106), Expect = 4.500e-6
Identity = 33/101 (32.67%), Postives = 42/101 (41.58%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWE--NSHKNNPLLTSYSWSYNEGVVSCDQ-NTKPRQKLCKCDQGITYCVR 388
            +E    +K    R+    + YG YCG G   G P D  D CC  HD CY   E    H        Y W+  +    CD    K  + LCKCD+    C+R
Sbjct:   32 LELAGAIKCSTGRSALAYMMYGCYCGLGG-QGWPRDRADWCCHRHDCCYGDAELLGCHTKT---AQYHWTCEDRKAECDDLKDKCEKMLCKCDREAAKCLR 128
[Close]
BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Match: SMESG000079712.1 (SMESG000079712.1)

HSP 1 Score: 295.819 bits (756), Expect = 1.092e-103
Identity = 140/140 (100.00%), Postives = 140/140 (100.00%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF 514
            IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF
Sbjct:   22 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF 161
[Close]
BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Match: SMESG000079712.1 (SMESG000079712.1)

HSP 1 Score: 284.648 bits (727), Expect = 1.055e-99
Identity = 135/135 (100.00%), Postives = 135/135 (100.00%), Query Frame = 2 
Query:  110 MLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF 514
            MLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF
Sbjct:    1 MLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF 135
[Close]
BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Match: SMESG000075160.1 (SMESG000075160.1)

HSP 1 Score: 145.976 bits (367), Expect = 1.443e-44
Identity = 62/139 (44.60%), Postives = 88/139 (63.31%), Query Frame = 2 
Query:   98 EFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRNTEQDTSLFVNF 514
            +FN M+K++   NP + +GYG YCG+G+L G P+D+ID CCK HD+CY+ +   +  NPLLT Y WS     + C  N    Q LCKCD+ I YC+RDN   FN  HY+RQ  +RA +  LN + K++  Q   +F+N+
Sbjct:   28 QFNLMVKYITGLNPGIFIGYGNYCGEGSLSGNPVDKIDECCKRHDECYDVFLKKYNANPLLTVYKWSLAGKEIWCKNNPVKLQTLCKCDKDIAYCLRDNLAHFNKGHYKRQNFQRAVISFLNTLIKKSYSQKQKMFINY 166
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BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Match: SMESG000025902.1 (SMESG000025902.1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.832e-26
Identity = 48/129 (37.21%), Postives = 71/129 (55.04%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNN-PLLTSYSWSYN----EGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNL 466
            +E NNML+  L  NP  LVGYG YCG GN    PID ID CCK+HD+C+  W   +++    LT Y W+      +  + C + T+ ++ LC+CD+    CV+  +  FN +HY      R  + + N+
Sbjct:   22 LELNNMLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGRQTRKKKDLCQCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNI 150
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BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Match: SMESG000025979.1 (SMESG000025979.1)

HSP 1 Score: 98.5969 bits (244), Expect = 4.555e-26
Identity = 48/129 (37.21%), Postives = 69/129 (53.49%), Query Frame = 2 
Query:   95 IEFNNMLKWVLKRNPSLLVGYGRYCGKGNLPGKPIDEIDSCCKVHDDCYETWENSHKNN-PLLTSYSWSYN----EGVVSCDQNTKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNL 466
            +E NNML+  L  NP  LVGYG YCG GN    PID ID CCK+HD+C+  W   +++    LT Y W+      +  + C   T+ ++ LC CD+    CV+  +  FN +HY      R  + + N+
Sbjct:   22 LELNNMLRKYLHINPGKLVGYGNYCGPGNNGKAPIDAIDMCCKIHDNCFGLWSEKYEDTKTYLTKYKWTLTMHGIKKFIVCGSQTRKKKDLCHCDKEFVICVKSKWHRFNHDHYSDTKNPRKFITSFNI 150
The following BLAST results are available for this feature:
BLAST of Acidic phospholipase A2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PLA2G53.134e-1233.94phospholipase A2 group V [Source:HGNC Symbol;Acc:H... [more]
PLA2G2A2.492e-1131.19phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G2A2.492e-1131.19phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G2A5.446e-1031.73phospholipase A2 group IIA [Source:HGNC Symbol;Acc... [more]
PLA2G1B8.018e-1031.73phospholipase A2 group IB [Source:HGNC Symbol;Acc:... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Y69A2AL.21.410e-837.04Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q... [more]
C07E3.97.027e-633.33Phospholipase A(2) [Source:UniProtKB/TrEMBL;Acc:Q... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acidic phospholipase A2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
pla2g1b1.148e-1033.90phospholipase A2, group IB (pancreas) [Source:ZFIN... [more]
zgc:921622.511e-732.11zgc:92162 [Source:ZFIN;Acc:ZDB-GENE-040718-41][more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
cntln1.707e-932.38centlein [Source:Xenbase;Acc:XB-GENE-953151][more]
sgtb2.945e-828.32small glutamine-rich tetratricopeptide repeat (TPR... [more]
eml67.551e-833.03echinoderm microtubule associated protein like 6 [... [more]
eml61.069e-733.03echinoderm microtubule associated protein like 6 [... [more]
eml61.143e-732.11echinoderm microtubule associated protein like 6 [... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Pla2g55.233e-1436.36phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g55.233e-1436.36phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g55.233e-1436.36phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g55.233e-1436.36phospholipase A2, group V [Source:MGI Symbol;Acc:M... [more]
Pla2g107.133e-1034.69phospholipase A2, group X [Source:MGI Symbol;Acc:M... [more]
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BLAST of Acidic phospholipase A2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P0DMT3|PA2HS_ECHCO1.567e-1632.41Phospholipase A2 homolog ECO_00035 OS=Echis colora... [more]
sp|Q8AXW7|PA2B_MICCO2.145e-1641.41Basic phospholipase A2 OS=Micrurus corallinus OX=5... [more]
sp|P0DMT1|PA2HS_ECHPL2.608e-1632.41Phospholipase A2 homolog EPL_00195 OS=Echis pyrami... [more]
sp|Q71QE8|PA2BN_CROVV9.322e-1635.78Basic phospholipase A2 Cvv-N6 OS=Crotalus viridis ... [more]
sp|P0DPT7|PA21_SCODE1.014e-1537.93Phospholipase A2 SSD387 OS=Scolopendra dehaani OX=... [more]
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BLAST of Acidic phospholipase A2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U3F5887.225e-1542.27Phospholipase A2 22 OS=Micrurus fulvius OX=8637 PE... [more]
A0A194ARB49.026e-1542.27Phospholipase A2 6e OS=Micrurus tener OX=1114301 P... [more]
A0A194AT571.740e-1442.27Phospholipase A2 6f OS=Micrurus tener OX=1114301 P... [more]
A0A194AT611.894e-1442.27Phospholipase A2 6a OS=Micrurus tener OX=1114301 P... [more]
A0A194AS731.914e-1442.27Phospholipase A2 6d OS=Micrurus tener OX=1114301 P... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSAMXT00000030049.11.187e-830.36phospholipase A2-like [Source:NCBI gene;Acc:103028... [more]
ENSAMXT00000031293.11.883e-830.36phospholipase A2-like [Source:NCBI gene;Acc:103028... [more]
ENSAMXT00000000319.26.472e-729.91phospholipase A2 [Source:NCBI gene;Acc:103024514][more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSPMAT00000008226.16.071e-1234.02pep scaffold:Pmarinus_7.0:GL476891:263168:265744:1... [more]
ENSPMAT00000008209.14.392e-1131.86pep scaffold:Pmarinus_7.0:GL476891:251313:253691:-... [more]
ENSPMAT00000008454.12.650e-730.97pep scaffold:Pmarinus_7.0:GL483377:2289:3737:-1 ge... [more]
ENSPMAT00000008199.15.219e-728.18pep scaffold:Pmarinus_7.0:GL476891:241341:242643:-... [more]
ENSPMAT00000008463.16.688e-729.57pep scaffold:Pmarinus_7.0:GL483377:12502:14271:-1 ... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Acidic phospholipase A2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 5
Match NameE-valueIdentityDescription
EDO347141.777e-1039.13Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO435043.263e-1032.43Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO384221.261e-736.84Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407202.461e-727.73Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO407426.914e-628.07Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Acidic phospholipase A2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2
Match NameE-valueIdentityDescription
ENSORLT00000032129.13.090e-732.65phospholipase A2 group IB [Source:NCBI gene;Acc:10... [more]
zgc:921624.500e-632.67phospholipase A2 [Source:NCBI gene;Acc:101170929][more]
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BLAST of Acidic phospholipase A2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000079712.11.092e-103100.00SMESG000079712.1[more]
SMESG000079712.11.055e-99100.00SMESG000079712.1[more]
SMESG000075160.11.443e-4444.60SMESG000075160.1[more]
SMESG000025902.11.832e-2637.21SMESG000025902.1[more]
SMESG000025979.14.555e-2637.21SMESG000025979.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011291 ID=SMED30011291|Name=Acidic phospholipase A2|organism=Schmidtea mediterranea sexual|type=transcript|length=598bp
CTCAAAATTAATAACTGAAAGATATTGAAAAATGCTTTGCCGTTTAAAGT
TTTTAACGTTTTTTTTGTGTTTTACTCTATCATTAGGCTCATTGATAGAA
TTCAATAACATGCTTAAATGGGTATTGAAAAGAAACCCCTCGCTACTTGT
GGGCTATGGACGATATTGTGGTAAAGGAAATTTGCCTGGAAAGCCAATAG
ATGAAATTGACTCGTGCTGCAAAGTCCATGATGACTGTTATGAAACTTGG
GAAAATTCACATAAAAATAATCCCTTATTAACTAGCTATAGCTGGTCCTA
CAATGAAGGGGTTGTGTCCTGTGATCAGAACACTAAACCACGACAGAAGT
TGTGCAAATGTGACCAAGGCATAACTTATTGTGTTAGAGATAATTTCAAA
GCATTCAATCCCGAACATTACCGACGTCAAGGCATAGAGAGAGCGGCTCT
TGGAACATTAAATTTAATTTGGAAAAGAAATACTGAACAAGACACTTCGC
TATTTGTAAATTTTTAATTATTTCACTCAATGTCAACTTTTTAATTGTTT
ATTAAATATATTTACTCTTGTGAACTTATTCAATAGATCAGACTCAAT
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protein sequence of SMED30011291-orf-1

>SMED30011291-orf-1 ID=SMED30011291-orf-1|Name=SMED30011291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=162bp
MLCRLKFLTFFLCFTLSLGSLIEFNNMLKWVLKRNPSLLVGYGRYCGKGN
LPGKPIDEIDSCCKVHDDCYETWENSHKNNPLLTSYSWSYNEGVVSCDQN
TKPRQKLCKCDQGITYCVRDNFKAFNPEHYRRQGIERAALGTLNLIWKRN
TEQDTSLFVNF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0034638phosphatidylcholine catabolic process
GO:0016042lipid catabolic process
GO:0006629lipid metabolic process
GO:0006644phospholipid metabolic process
GO:0050482arachidonic acid secretion
Vocabulary: INTERPRO
TermDefinition
IPR036444PLipase_A2_dom_sf
IPR001211PLipase_A2
IPR016090PLipase_A2_dom
IPR033113PLipase_A2_His_AS
Vocabulary: molecular function
TermDefinition
GO:0004623phospholipase A2 activity
GO:0005509calcium ion binding
GO:0016787hydrolase activity
GO:0046872metal ion binding
Vocabulary: cellular component
TermDefinition
GO:0005576extracellular region
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001211Phospholipase A2PRINTSPR00389PHPHLIPASEA2coord: 103..119
score: 26.41
coord: 21..31
score: 33.75
coord: 85..99
score: 27.91
coord: 55..73
score: 53.58
IPR001211Phospholipase A2PANTHERPTHR11716PHOSPHOLIPASE A2 FAMILY MEMBERcoord: 18..130
IPR016090Phospholipase A2 domainSMARTSM00085PA-fincoord: 20..139
e-value: 6.4E-23
score: 92.1
IPR016090Phospholipase A2 domainPFAMPF00068Phospholip_A2_1coord: 21..129
e-value: 9.7E-25
score: 87.2
IPR016090Phospholipase A2 domainCDDcd00125PLA2ccoord: 20..132
e-value: 2.55387E-25
score: 91.5424
IPR036444Phospholipase A2 domain superfamilyGENE3DG3DSA:1.20.90.10coord: 16..137
e-value: 3.0E-28
score: 100.3
IPR036444Phospholipase A2 domain superfamilySUPERFAMILYSSF48619Phospholipase A2, PLA2coord: 20..130
IPR033113Phospholipase A2, histidine active sitePROSITEPS00118PA2_HIScoord: 62..69
NoneNo IPR availableSIGNALP_EUKSignalP-TMSignalP-TMcoord: 1..19
score: 0.625
NoneNo IPR availableTMHMMTMhelixcoord: 7..24