SMED30011283
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011283 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 8Note: Hover over icons to view figure legend
Homology
BLAST of SMED30011283 vs. Planmine SMEST
Match: SMESG000032426.1 (SMESG000032426.1) HSP 1 Score: 179.874 bits (455), Expect = 6.685e-53 Identity = 90/102 (88.24%), Postives = 93/102 (91.18%), Query Frame = 2 Query: 23 MIESPVKPKISMRHSRTRYKCRSQFQYVTSESYTNNVSEIDLKAFNEDFKLHNVKMLRIRKSTENDLEKESSDGLLIPKFDKELLAQYKISTVKQDSPIYIP 328 MIESPVKPKISMRHSRTR+K RSQ QY TSESYTN VSE DL+AFNEDFKL NVKMLRIRKSTENDLEKES D L IPKFDKELLAQYKISTVK+DSPIYIP Sbjct: 1 MIESPVKPKISMRHSRTRHKFRSQLQYATSESYTNKVSESDLRAFNEDFKLLNVKMLRIRKSTENDLEKESFDELFIPKFDKELLAQYKISTVKRDSPIYIP 102
BLAST of SMED30011283 vs. Planmine SMEST
Match: SMESG000036077.1 (SMESG000036077.1) HSP 1 Score: 78.9518 bits (193), Expect = 1.104e-35 Identity = 42/100 (42.00%), Postives = 59/100 (59.00%), Query Frame = 2 Query: 1706 CLRCCDICSKRKIDQTRTKEIFIPRYSSEFLEQIVMD---------KTYIVLMVDRFSTLISLTARYLKEH---FKTIFYSWIYRFGILKSKLTGRGQNF 1969 C+ C IC+ RKIDQ RTKEI +PR +FLEQI++D K Y++++ D F L+SL+A + I +WIYRFG +S LT +G+ F Sbjct: 169 CINKCKICASRKIDQGRTKEILLPRTGEQFLEQIIVDIAYMETKESKNYMIVIKDCFCKLVSLSAASTHDEETILNVILNNWIYRFGRPESILTDKGRIF 268 HSP 2 Score: 75.0998 bits (183), Expect = 1.104e-35 Identity = 51/163 (31.29%), Postives = 83/163 (50.92%), Query Frame = 3 Query: 1230 LANLAEYDF*LKYRKGEEHSNPSGLSRLKGMICTQRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSSRRISNFKSYEIC*KM---EMCIRIKLVFHST*KIY**M-----IINSRKEQQCLILVQETISGLLIITLHQELCHNGVKILFHYIERIFYWPKM 1694 +ANL+EYDF L+YRKGEE N G+S L IC+ Q H+++KE K RY+ S++ + + K ++I + + EI + E I + + S K + ++ + + ++V E L+ H ELCH G+K Y++ F+WP M Sbjct: 1 MANLSEYDFALQYRKGEERGNADGVSHLNNTICSHCQMEHKDAKEAKCRTRYINSLQGSSNIMKII-KQKQIEDRVTSEIISHLNGNEAYIFYETISSSIFKYLKILQIQDDVLMINTDGKLAVVVPEFCVKSLVNYFHIELCHLGIKKNLFYLKDFFFWPSM 162 HSP 3 Score: 37.3502 bits (85), Expect = 1.104e-35 Identity = 16/24 (66.67%), Postives = 18/24 (75.00%), Query Frame = 1 Query: 2008 SGPYQRQSNGLAESIIRALREPLP 2079 S PYQ QSNGLAE IIR +R+ L Sbjct: 287 SSPYQHQSNGLAERIIRTVRDMLA 310
BLAST of SMED30011283 vs. Planmine SMEST
Match: SMESG000049233.1 (SMESG000049233.1) HSP 1 Score: 113.235 bits (282), Expect = 1.764e-35 Identity = 68/194 (35.05%), Postives = 100/194 (51.55%), Query Frame = 3 Query: 1176 FHVHEHHK-----NANQSTISK----WLANLAEYDF*LKYRKGEEHSNPSGLSRLKGMICTQRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSSRRISNFKSYEIC*KMEMCIR-----------IKLVFHST*KIY**MIINSRKEQQCLILVQETISGLLIITLHQELCHNGVKILFHYIERIFYWPKMQ 1697 F V HK N + IS W+ NL++YDF LKY KGE+HSN GLSR+ GM+C+Q QTRHE +KE K ++++ I++TE M + + + F+ KM+ C+ K+ ++I K L+LV + L+ +H+++C GVK L HYI IFYWP +Q Sbjct: 200 FMVRTDHKALIFLNTTKKAISPQFQTWMVNLSDYDFELKYHKGEDHSNADGLSRIGGMLCSQCQTRHENAKEAKVRVKHICMIQKTEVMHQLFEAQESNKQFE------KMKKCVNNGNFGDFDKKDFFFKLRDNLKMNDGLLI-VNKNGMDLLLVPIEFTQQLVTKIHEKMCRVGVKKLVHYINNIFYWPNLQ 386 HSP 2 Score: 56.6102 bits (135), Expect = 1.764e-35 Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 2 Query: 1706 CLRCCDICSKRKIDQTRTKEIFIPRYSSEFLEQIVMD---------KTYIVLMVDRFSTLISLTARYLKEH---FKTIFYSWIYRFGI 1933 CL+ C C++RK++Q +TKEI +P SEF +QIV+D K Y V++ D FS L+SLTA ++ ++ + + W G+ Sbjct: 392 CLQKCQECARRKVNQEQTKEILLPHVISEFSDQIVIDIAYMKPIATKKYAVVIKD-FSKLVSLTATSKQDDKAIYRVLLHQWRIHQGV 478
BLAST of SMED30011283 vs. Planmine SMEST
Match: SMESG000049233.1 (SMESG000049233.1) HSP 1 Score: 113.235 bits (282), Expect = 1.914e-35 Identity = 68/194 (35.05%), Postives = 100/194 (51.55%), Query Frame = 3 Query: 1176 FHVHEHHK-----NANQSTISK----WLANLAEYDF*LKYRKGEEHSNPSGLSRLKGMICTQRQTRHEESKEGKSNIRYVYSIKRTETMEKYWSSSRRISNFKSYEIC*K-----------MEMCIRIKLVFHST*KIY**MIINSRKEQQCLILVQETISGLLIITLHQELCHNGVKILFHYIERIFYWPKMQ 1697 F V HK N + IS W+ NL++YDF LKY KGE+HSN GLSR+ GM+C+Q QTRHE +KE K ++++ I++TE M + + + F+ + C +R L + +I+N K L+LV + L+ +H+++C GVK L HYI IFYWP +Q Sbjct: 228 FMVRTDHKALIFLNTTKKAISPQFQTWMVNLSDYDFELKYHKGEDHSNADGLSRIGGMLCSQCQTRHENAKEAKVRVKHICMIQKTEVMHQLFEAQESNKQFEKMKKCVNNGNFGDFDKKDFFFKLRDNLKMNDG-----LLIVN--KNGMDLLLVPIEFTQQLVTKIHEKMCRVGVKKLVHYINNIFYWPNLQ 414 HSP 2 Score: 56.6102 bits (135), Expect = 1.914e-35 Identity = 32/88 (36.36%), Postives = 51/88 (57.95%), Query Frame = 2 Query: 1706 CLRCCDICSKRKIDQTRTKEIFIPRYSSEFLEQIVMD---------KTYIVLMVDRFSTLISLTARYLKEH---FKTIFYSWIYRFGI 1933 CL+ C C++RK++Q +TKEI +P SEF +QIV+D K Y V++ D FS L+SLTA ++ ++ + + W G+ Sbjct: 420 CLQKCQECARRKVNQEQTKEILLPHVISEFSDQIVIDIAYMKPIATKKYAVVIKD-FSKLVSLTATSKQDDKAIYRVLLHQWRIHQGV 506
BLAST of SMED30011283 vs. Planmine SMEST
Match: SMESG000038612.1 (SMESG000038612.1) HSP 1 Score: 81.6481 bits (200), Expect = 9.885e-34 Identity = 41/97 (42.27%), Postives = 59/97 (60.82%), Query Frame = 2 Query: 1706 CLRCCDICSKRKIDQTRTKEIFIPRYSSEFLEQIVMD---------KTYIVLMVDRFSTLISLTARYLKEH---FKTIFYSWIYRFGILKSKLTGRG 1960 C+ C IC+ RKID+ RTKEI +PR FLEQIV+D K Y+++++DRF+ +SL+A ++ I +WIYRFG +S LT +G Sbjct: 483 CVNKCKICASRKIDEGRTKEILLPRTGERFLEQIVVDIEYMETKESKKYMIVIIDRFNKFVSLSATSTQDEAIILNAILNNWIYRFGRPESILTDKG 579 HSP 2 Score: 63.5438 bits (153), Expect = 9.885e-34 Identity = 49/171 (28.65%), Postives = 79/171 (46.20%), Query Frame = 3 Query: 1197 KNANQSTISKWLANLAEYDF*LKYRKGEEHSNPSGLSRLKGMICTQRQTRHEESKEGKSNIRY----VYSIKRTETMEKYWSSSRRISNFKSYEIC*KMEMCIRIKLVFHST*KIY**MIINSRKEQQCLILVQETISGLLIITLHQELCHNGVKILFHYIERIFYWPKMQ 1697 K A W+ANL+EYDF L+YRK ++H N G+S L IC+ Q ++++KE K R + I E Y + S I FK +I + I+ + ++IN+ + + ++V + L+ H ELCH G+ Y++ F+W M Sbjct: 327 KKAISPQFQTWMANLSEYDFALQYRKEKKHGNADGMSGLNNTICSNCQMENKDAKEAKCRTRVTSEIISHINGNEAHISYETISSNI--FKYLKI-----LNIQDDV-----------LMINT--DGKLAVVVPDWYVKSLVNYFHFELCHLGIMKTLFYLKDFFFWSFMN 477 HSP 3 Score: 39.6614 bits (91), Expect = 9.885e-34 Identity = 22/57 (38.60%), Postives = 30/57 (52.63%), Query Frame = 1 Query: 2008 SGPYQRQSNGLAESIIRALREP-----------------LPRIEFNINTSWQRASLF 2127 S PY+ QSNGLAE IIR +R LP+IEF++N + Q ++ F Sbjct: 601 SSPYEHQSNGLAERIIRTVRYMLATSLTEIKTKNNWFRLLPKIEFSLNATIQNSTKF 657 The following BLAST results are available for this feature:
BLAST of SMED30011283 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30011283 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30011283 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30011283 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30011283 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011283 ID=SMED30011283|Name=SMED30011283|organism=Schmidtea mediterranea sexual|type=transcript|length=2623bpback to top protein sequence of SMED30011283-orf-1 >SMED30011283-orf-1 ID=SMED30011283-orf-1|Name=SMED30011283-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=191bp MIESPVKPKISMRHSRTRYKCRSQFQYVTSESYTNNVSEIDLKAFNEDFKback to top Annotated Terms
The following terms have been associated with this transcript:
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