Granulin precursor
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011250 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 16Note: Hover over icons to view figure legend
Homology
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 232.261 bits (591), Expect = 6.315e-71 Identity = 138/365 (37.81%), Postives = 189/365 (51.78%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPI-------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
T G+ SCCP+ AV C D HCCP+ C+ + +C + +S+ ++ CPD Q +C D S+CCVM DG++ CCP A CC D+ HCCP C+L C + K++ + + A+S+ S+ CPD S C G+TCC L G YGCCP NA CCSD L CCPQ+TVC+L C SK + L+ + V V C D + C DG TCC+L +G ++GCCP++ A CC D IHCCP C+ KGTC+ HQ +E + + L + C N S C TCC ++CCPY VCC+D HCCP C+ C + ++
Sbjct: 75 FTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-----------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSS-SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSG-AWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRW 425 HSP 2 Score: 183.726 bits (465), Expect = 1.929e-52 Identity = 112/304 (36.84%), Postives = 150/304 (49.34%), Query Frame = 2
Query: 398 KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
K S L C + C G SC G +CCP+ AV C D HCCP+ C+ +C + S +I+CPD C +TCC++ DGS+GCCP A CC D++ CCP C+L C + T L K NR V+CPDA+ +C DG TCC+LP+G YGCCP ATCCSD +HCCPQ+TVC++ + C + A L K+ V +KC C TCC + ++ CCP+ AVCC DH HCCP +C +C++
Sbjct: 48 KWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGNN-----------SVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGT-HPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSG-KYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQG 338
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 234.572 bits (597), Expect = 3.424e-70 Identity = 140/350 (40.00%), Postives = 187/350 (53.43%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPI-------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
T G+ SCCP+ AV C D HCCP+ C+ + +C + +S+ ++ CPD Q +C D S+CCVM DG++ CCP A CC D+ HCCP C+L C + K++ + + A+S+ S+ CPD S C G+TCC L G YGCCP NA CCSD L CCPQ+TVC+L C SK + L+ + V V C D + C DG TCC+L +G ++GCCP++ A CC D IHCCP C+ KGTC+ HQ +E + + L + C N S C TCC + + CCP P AVCCSDH HCCP +C
Sbjct: 75 FTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-----------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSS-SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSG-AWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC 410 HSP 2 Score: 227.639 bits (579), Expect = 1.106e-67 Identity = 140/370 (37.84%), Postives = 193/370 (52.16%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSI-------LSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF------QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE-IKISPILVESIKCPNGSLCGQK------GTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFP 1297
V DGS+ CCP A CC D+ HCCP C+L C + + K+PA SV+CPD +S+C DGS+CC + G + CCP +A CCSD HCCPQ+TVC+L C SK + T + K+PA + ++C D +C G TCC L G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P + R + D C TCC+L +G+ +GCCP A CCSD HCCPQ C + +G C +E + ++ P S+ P C Q TCCPS S++CC P+AVCC D HCCP +C++ +SC+K ++ P
Sbjct: 141 VMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRC--ITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGE-WGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQP 504 HSP 3 Score: 210.69 bits (535), Expect = 2.870e-61 Identity = 126/361 (34.90%), Postives = 177/361 (49.03%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV-------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE----IKISP-ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G Y CCP +A CCSD HCCPQ+T C+L + C S + T + K+PA ++ V C + C DG +CC + G + CCP++ AVCC D HCCP C+ GTC+ +V K L +P L + P D S+C + TCC L+ G +GCCP AVCCSD CCPQ C + + ++ S ++ + P R I D C GQTCC G S+ CC A CC D+ HCCP CN+ +C+ + + + + SP + V+ ++C G C TCC ++CCPY VCC+D HCCP C+ C + ++
Sbjct: 227 GKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-----VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCP-SLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRW 580 HSP 4 Score: 183.341 bits (464), Expect = 4.202e-51 Identity = 112/304 (36.84%), Postives = 150/304 (49.34%), Query Frame = 2
Query: 398 KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
K S L C + C G SC G +CCP+ AV C D HCCP+ C+ +C + S +I+CPD C +TCC++ DGS+GCCP A CC D++ CCP C+L C + T L K NR V+CPDA+ +C DG TCC+LP+G YGCCP ATCCSD +HCCPQ+TVC++ + C + A L K+ V +KC C TCC + ++ CCP+ AVCC DH HCCP +C +C++
Sbjct: 48 KWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN-----------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGT-HPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSG-KYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQG 338
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 201.445 bits (511), Expect = 3.692e-59 Identity = 124/366 (33.88%), Postives = 175/366 (47.81%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV-------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE----IKISP-ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
T G+ SCCP+ AV C D HCCP+ C+ + +C + +S+ ++ CPD Q +C D S+CCVM DG++ CCP AVCC D HCCP C+ GTC+ +V K L +P L + P D S+C + TCC L+ G +GCCP AVCCSD CCPQ C + + ++ S ++ + P R I D C GQTCC G S+ CC A CC D+ HCCP CN+ +C+ + + + + SP + V+ ++C G C TCC ++CCPY VCC+D HCCP C+ C + ++
Sbjct: 75 FTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-----------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-----VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPS-LGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRW 423 HSP 2 Score: 167.548 bits (423), Expect = 1.767e-46 Identity = 106/313 (33.87%), Postives = 147/313 (46.96%), Query Frame = 2
Query: 398 KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT------FQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE-IKISPILVESIKCPNGSLCGQK------GTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFP 1297
K S L C + C G SC G +CCP+ AV C D HCCP+ C+ +C + S +I+CPD C +TCC++ DGS+GCCP AVCC D + CCP C+ GTC+ + + K P + R + D C TCC+L +G+ +GCCP A CCSD HCCPQ C + +G C +E + ++ P S+ P C Q TCCPS S++CC P+AVCC D HCCP +C++ +SC+K ++ P
Sbjct: 48 KWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN-----------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGE-WGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQP 347
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 197.978 bits (502), Expect = 1.809e-58 Identity = 119/304 (39.14%), Postives = 166/304 (54.61%), Query Frame = 2
Query: 428 SVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT------FQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE-IKISPILVESIKCPNGSLCGQK------GTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFP 1297
SV+CPD +S+C DGS+CC + G + CCP +A CCSD HCCPQ+TVC+L C SK + T + K+PA + ++C D +C G TCC L G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P + R + D C TCC+L +G+ +GCCP A CCSD HCCPQ C + +G C +E + ++ P S+ P C Q TCCPS S++CC P+AVCC D HCCP +C++ +SC+K ++ P
Sbjct: 19 SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGE-WGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQP 318 HSP 2 Score: 188.734 bits (478), Expect = 7.833e-55 Identity = 116/327 (35.47%), Postives = 162/327 (49.54%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV-------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE----IKISP-ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPY 1192
G Y CCP +A CCSD HCCPQ+T C+L + C S + T + K+PA ++ V C + C DG +CC + G + CCP++ AVCC D HCCP C+ GTC+ +V K L +P L + P D S+C + TCC L+ G +GCCP AVCCSD CCPQ C + + ++ S ++ + P R I D C GQTCC G S+ CC A CC D+ HCCP CN+ +C+ + + + + SP + V+ ++C G C TCC ++CCPY
Sbjct: 41 GKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-----VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPS-LGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPY 360 HSP 3 Score: 164.851 bits (416), Expect = 8.078e-46 Identity = 96/225 (42.67%), Postives = 125/225 (55.56%), Query Frame = 2
Query: 605 KEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPI-------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
K++ + + A+S+ S+ CPD S C G+TCC L G YGCCP NA CCSD L CCPQ+TVC+L C SK + L+ + V V C D + C DG TCC+L +G ++GCCP++ A CC D IHCCP C+ KGTC+ HQ +E + + L + C N S C TCC + + CCP P AVCCSDH HCCP +C
Sbjct: 3 KKLPAQRTNRAVALSS-SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSG-AWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC 224 HSP 4 Score: 98.5969 bits (244), Expect = 5.534e-22 Identity = 61/172 (35.47%), Postives = 85/172 (49.42%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSI---LSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSN 751
T G + CCP AVCCSD QHCCPQ C + +G C + V K+ +S+ + C + C G +CC G++ACC AVCC D+QHCCP CN+ A +C+ KEVV+ + P + +EC +G C TCC + + CCP+
Sbjct: 196 TSGEWGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCE---KEVVSAQPATFLARSPHVGVKDVECGEG-HFCHDNQTCCRDNRQGWACCPYRQ 362
BLAST of Granulin precursor vs. Ensembl Human
Match: GRN (granulin precursor [Source:HGNC Symbol;Acc:HGNC:4601]) HSP 1 Score: 74.7146 bits (182), Expect = 3.773e-15 Identity = 45/126 (35.71%), Postives = 59/126 (46.83%), Query Frame = 2
Query: 398 KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAV 757
K S L C + C G SC G +CCP+ A CC D+ HCCP C+L C + K++ + + A+S+ S+ CPD S C G+TCC L G YGCCP NA
Sbjct: 48 KWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSS-SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNAT 172 HSP 2 Score: 74.3294 bits (181), Expect = 4.238e-15 Identity = 44/108 (40.74%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 686 CKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAAT 988
C G +C G+ CCPF A CC D++ CCP C+L C + T L K NR V+CPDA+ +C DG TCC+LP+G YGCCP AT
Sbjct: 67 CSAGHSCIFTVSGTSSCCPFPEASCCEDRVHCCPHGAFCDLVHTRCITPTGT-HPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGK-YGCCPMPNAT 172 HSP 3 Score: 63.929 bits (154), Expect = 2.193e-11 Identity = 37/100 (37.00%), Postives = 51/100 (51.00%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSI-------LSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAV 526
T G+ SCCP+ A CC D+ HCCP C+L C + + K+PA SV+CPD +S+C DGS+CC + G + CCP +A
Sbjct: 75 FTVSGTSSCCPFPEASCCEDRVHCCPHGAFCDLVHTRC--ITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNAT 172 HSP 4 Score: 56.225 bits (134), Expect = 8.401e-9 Identity = 36/108 (33.33%), Postives = 49/108 (45.37%), Query Frame = 2
Query: 911 QCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---------DHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAV 1204
CS G +C +G S CCP+ A+CC D++HCCP C++ C + A + L S+ CP+ S C TCC + Y CCP PNA
Sbjct: 66 HCSAGHSCIFTVSGTS-SCCPFPEASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNAT 172
BLAST of Granulin precursor vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 111.694 bits (278), Expect = 4.735e-27 Identity = 84/246 (34.15%), Postives = 106/246 (43.09%), Query Frame = 2
Query: 671 DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNIN---------------KGTCDHQAEALEIKISPIL----------------VESIKCPNGSLCGQKGTCC----PSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
D + C TCC L D ++GCCP NAVCC D+ CCP T C+ C K KKP+ KT + N N V+CPD +C DG TCC L G SYGCCP A CC+D +HCCP C+ T H+ A+E V+ I C G C K TCC + CCP NA+CC + CCP C ++ C+K+
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPAR--KTVERNQFNEVVCPDKASKCPDGSTCCLLEQG-SYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGKTCPAKTTCCNVRGKNGEPKTMCCPLSNAICCEN--TCCPAGYHC-VDGGKCEKH 338 HSP 2 Score: 108.612 bits (270), Expect = 5.698e-26 Identity = 83/268 (30.97%), Postives = 120/268 (44.78%), Query Frame = 2
Query: 395 IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVC-------------NLPAGTCDSKTKKPSLLIKTFQ---------------PNPVNRVICPDAKYQCSDGQTCCKLPTGDSY---GCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKI 1087
+ L V + + C +++C D +CC + D + CCP +AVCC D+ HCCP T C+ C ++ V MK K PA + + CPD +S C G+TCCLL GSYGCCP NAVCC+D L CCP C +L S +KP++ + + + V+ + C K C TCC + + CCP S A CC + CCP C ++ G C+ A+ + +
Sbjct: 84 LFLAVFLCRVTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVP-MKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVGK-TCPAKTTCCNVRGKNGEPKTMCCPLSNAICCENT--CCPAGYHC-VDGGKCEKHAKTMRTRF 346 HSP 3 Score: 94.3597 bits (233), Expect = 5.277e-21 Identity = 77/232 (33.19%), Postives = 107/232 (46.12%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSIL------SVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSA----GTCDSKLKEVVTKMKLKIPAISNLS-----------------------IECPDGSSTCKTGATCCLLSDGSYG-----CCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTK 838
D ++ CCP +AVCC D+ HCCP T C+ C ++ V +K K PA + VVCPD+ S+C DGS+CC++ G++ CCP +AVCC+D HCCP C+ L++ + + PA+ LS I C G TC TCC + G G CCP SNA+CC + +CCP C + G C+ K
Sbjct: 115 DNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVP-MKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCHGQFCSQNFAMIPHLRKFASTSIHRKPAVEFLSEEEEDDDDEESTSSEDPESDVDPIACGVG-KTCPAKTTCCNVR-GKNGEPKTMCCPLSNAICCEN--TCCPAGYHC-VDGGKCEKHAK 340 HSP 4 Score: 87.8113 bits (216), Expect = 8.598e-19 Identity = 60/154 (38.96%), Postives = 79/154 (51.30%), Query Frame = 2
Query: 899 DAKYQCSDGQTCCKLPTGD-SYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIK------ISPILVESIKCPN-GSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-----LLKFPIINFH 1312
DA+ +CSD +TCCKL GD ++GCCP A CC D+ HCCP T C+ C D + ++ K + + CP+ S C TCC SY CCP PNAVCC+D HCCPN +C + + C +N L KF + H
Sbjct: 99 DAETECSDDETCCKL--GDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC--HGQFCSQNFAMIPHLRKFASTSIH 248 HSP 5 Score: 51.2174 bits (121), Expect = 7.438e-7 Identity = 24/57 (42.11%), Postives = 32/57 (56.14%), Query Frame = 2
Query: 1100 VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
V + +C + C TCC ++++ CCP PNAVCC D SHCCP T+C C
Sbjct: 93 VTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARC 149
BLAST of Granulin precursor vs. Ensembl Celegans
Match: pgrn-1 (ProGRaNulin homolog [Source:UniProtKB/TrEMBL;Acc:Q7JKP2]) HSP 1 Score: 105.916 bits (263), Expect = 3.404e-25 Identity = 56/129 (43.41%), Postives = 73/129 (56.59%), Query Frame = 2
Query: 437 CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCN 805
C +++C D +CC + D + CCP +AVCC D+ HCCP T C+ C ++ V MK K PA + + CPD +S C G+TCCLL GSYGCCP NAVCC+D L CCP C+
Sbjct: 98 CDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHV-PMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCH 225 HSP 2 Score: 105.531 bits (262), Expect = 5.128e-25 Identity = 58/130 (44.62%), Postives = 70/130 (53.85%), Query Frame = 2
Query: 671 DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCN 1036
D + C TCC L D ++GCCP NAVCC D+ CCP T C+ C K KKP+ KT + N N V+CPD +C DG TCC L G SYGCCP A CC+D +HCCP C+
Sbjct: 99 DAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPAR--KTVERNQFNEVVCPDKASKCPDGSTCCLLEQG-SYGCCPVPNAVCCADMLHCCPNGFTCH 225 HSP 3 Score: 88.5817 bits (218), Expect = 3.738e-19 Identity = 60/154 (38.96%), Postives = 79/154 (51.30%), Query Frame = 2
Query: 899 DAKYQCSDGQTCCKLPTGD-SYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIK------ISPILVESIKCPN-GSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-----LLKFPIINFH 1312
DA+ +CSD +TCCKL GD ++GCCP A CC D+ HCCP T C+ C D + ++ K + + CP+ S C TCC SY CCP PNAVCC+D HCCPN +C + + C +N L KF + H
Sbjct: 99 DAETECSDDETCCKL--GDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGADEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTC--HGQFCSQNFAMIPHLRKFASTSIH 248 HSP 4 Score: 87.0409 bits (214), Expect = 1.319e-18 Identity = 47/112 (41.96%), Postives = 64/112 (57.14%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSIL------SVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCN 574
D ++ CCP +AVCC D+ HCCP T C+ C E +K K PA + VVCPD+ S+C DGS+CC++ G++ CCP +AVCC+D HCCP C+
Sbjct: 115 DNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARCIGA-DEKHVPMKKKKPARKTVERNQFNEVVCPDKASKCPDGSTCCLLEQGSYGCCPVPNAVCCADMLHCCPNGFTCH 225 HSP 5 Score: 51.6026 bits (122), Expect = 6.246e-7 Identity = 24/57 (42.11%), Postives = 32/57 (56.14%), Query Frame = 2
Query: 1100 VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
V + +C + C TCC ++++ CCP PNAVCC D SHCCP T+C C
Sbjct: 93 VTAHQCDAETECSDDETCCKLGDNTWGCCPMPNAVCCDDRSHCCPTGTTCDPQGARC 149
BLAST of Granulin precursor vs. Ensembl Celegans
Match: T02B11.8 (pep chromosome:WBcel235:V:877530:878667:-1 gene:WBGene00044776.1 transcript:T02B11.8a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:T02B11.8) HSP 1 Score: 44.669 bits (104), Expect = 7.657e-6 Identity = 21/60 (35.00%), Postives = 29/60 (48.33%), Query Frame = 2
Query: 1133 CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFH 1312
C + CCP N+ Y CCP+ CC +HCCP SC+ +C + L P+ F
Sbjct: 34 CDEYAVCCPIGNEQYGCCPFTGGTCCPGTNHCCPPGFSCT-TIGTCKRTTTLSNPVSPFR 92
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grnb (granulin b [Source:ZFIN;Acc:ZDB-GENE-030131-7393]) HSP 1 Score: 239.195 bits (609), Expect = 4.209e-71 Identity = 150/396 (37.88%), Postives = 207/396 (52.27%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC--DSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC--------------DSKLKEVVTKMKLKIPAISN--LSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-------DSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESI-----------KCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC----DKNILLKFPIINFHP 1315
+T G Y CCP A CCSD HCC Q T C+L C + + + V K++ K+ A VVCPD +S+C D ++CC M DG + CCP +AVCC D++HCCPQ T C+L C D++ ++ + K + +PA N + CPD S C TCCLL GSYGCCP AVCCSD+ CCP+ T C+L TC + TK P++ + P V+ + CS G TCCK P G S+ CCP A CC D IHCCP+ T+CN+ +CD A + +S +E + KC S C + TCC ++ + CCP P AVCC DH HCCP+ + C++ ++C D + + P++ P
Sbjct: 36 LTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVNKTHVLDWVEKVEAKLQA-----VVCPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSANGVSEMATKIPAVTVL----KPKEEVVPCNETVACSSGTTCCKTPEG-SWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPA---GLSVSVPWMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVKKIP 418 HSP 2 Score: 231.491 bits (589), Expect = 2.930e-68 Identity = 144/391 (36.83%), Postives = 207/391 (52.94%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC--------------DSVLKEVVTKIKLKVPAS--SILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS-----IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCD-------SKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESI----------KCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
DG + CCP +AVCC D++HCCPQ T C+L C D+ ++ + K + +PA +I V+CPD+ S+C + ++CC++ G++ CCP AVCCSD++HCCP+ T C+L TC S V++M KIPA++ L + C + + C +G TCC +GS+ CCP AVCC D + CCP+ T+CN+ A +CD S + K P P + D C TCCKL +GD +GCCP A CC D +HCCP +VCN+ TC+ +++ ++IS +V+ I C S C TCC + S++CCP P AVCC+D HCCP +C L SC ++ L P
Sbjct: 113 DGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETGSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLS--ANGVSEMATKIPAVTVLKPKEEVVPC-NETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVSVPWMEKVSTKPIAPPPNKKC---DESSSCPGESTCCKLSSGD-WGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDS-GLRISVPMVKKIPAVSVPSQKQNCDETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEP 495 HSP 3 Score: 216.083 bits (549), Expect = 1.927e-62 Identity = 137/369 (37.13%), Postives = 194/369 (52.57%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS---IECP-----DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSD------GQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQA--EALEIKISPILVESIKCPNGSLCGQKGTCCPSAN--------DSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI 1282
GSY CCP AVCCSD++HCCP+ T C+L TC S V+++ K+PA ++L V C + C G++CC +G++ACCP AVCC D HCCP+ T+CN++A +CD + + +P + +S I P D SS+C +TCC LS G +GCCP AVCC D + CCP +VCN+ A TC++ + L I + V P K C + G TCCKL +G S+ CCP A CC+D+ HCCPQ C++ + +C + P L E+ + + +C + T CP + + CCP P AVCC D HCCP+ +C+ SC K +
Sbjct: 207 GSYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSA--NGVSEMATKIPAVTVLKPKEEVVPCNETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDP-----AGLSVSVPWMEKVSTKPIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDS-GLRISVPMVKKIPAVSVPSQKQNCDETSSCPTGTTCCKLTSG-SWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPALRETQRVEDRHMCDAQ-TSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGL 565 HSP 4 Score: 212.231 bits (539), Expect = 3.919e-61 Identity = 125/317 (39.43%), Postives = 166/317 (52.37%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK--LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-----------------QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPILV-----ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPI 1300
+++CPD C+DG++CC+ G + CCP A CCSD HCC Q T+C+L C +K + + V K++ K+ A+ CPDG S C TCC + DG +GCCP NAVCC D+ CCPQ T C+L C S T S ++ F + N + VICPD +C + TCC L TG SYGCCP A CCSD+ HCCP+ T C++ TC + + KI + V E + C C TCC + S++CCP P AVCC DH HCCP T C++ SCD L +
Sbjct: 19 ALICPDGGMCEDGNTCCLTPSGGYGCCPLPHAECCSDHLHCCYQGTLCDLEHSKCVNKTHVLDWVEKVEAKLQAVV-----CPDGESECPDDTTCCQMPDGGWGCCPMKNAVCCDDRKHCCPQGTTCDLVHSMCVSTTYGSSPFLRKFDARRRKPLEKNAVDLPAEVNNIREVICPDKISKCPEDTTCCLLETG-SYGCCPMPKAVCCSDQKHCCPEGTTCDLIHSTCLSANGVSEMATKIPAVTVLKPKEEVVPCNETVACSSGTTCCKTPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVSV 329 HSP 5 Score: 212.231 bits (539), Expect = 4.166e-61 Identity = 141/368 (38.32%), Postives = 190/368 (51.63%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCD-----SVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----DSKLKEVVTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQP------NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPILV--------ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK 1276
T +GS++CCP AVCC D HCCP+ T CN+ +CD SV + K+ K P + + C + S C S+CC ++ G++ CCP AVCC D HCCP +VCN++A TC DS L+ V +K KIPA+S S + D +S+C TG TCC L+ GS+ CCP AVCC+D+ CCPQ C+L +C ++ PS+ +P +R +C DA+ C TCC + +GCCP A CC D HCCP CN K +C HQ K + + E +KC + + C TCC + CCP AVCC DH HCCP C L +C+K
Sbjct: 283 TPEGSWACCPLPKAVCCEDHIHCCPEGTLCNVAASSCDDPAGLSVSVPWMEKVSTK-PIAPPPNKKCDESSSCPGESTCCKLSSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVK-KIPAVSVPSQKQNCDETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSC-VRSGLPSMAWFRKEPALRETQRVEDRHMC-DAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFTKKTARVWKSSDELLGHEDVKCDSSTSCPSGSTCCILPTGQWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEK 646 HSP 6 Score: 208.764 bits (530), Expect = 9.215e-60 Identity = 125/357 (35.01%), Postives = 182/357 (50.98%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV----LKEVVTKIKLKVPASSILSVV--CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP---DGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK-------TKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK 1276
+ G + CCP AVCC D HCCP + CN+ TC++V L+ V +K K+PA S+ S C + S C G++CC + G++ACCP AVCC+D++HCCPQ C+L+ +C + + + +E D ++C TCC ++ G +GCCP AVCC D CCP CN +C TKK + + K+ + + D+ C G TCC LPTG +GCCP A CC D HCCPQ +C + GTC+ + + + ++ + + I+C + C +CC A+ +++CCPY AVCC D HCCP C + C K
Sbjct: 364 SSGDWGCCPLPEAVCCEDHVHCCPHGSVCNVAAETCETVSDSGLRISVPMVK-KIPAVSVPSQKQNCDETSSCPTGTTCCKLTSGSWACCPMPQAVCCADQEHCCPQGYTCDLAQSSCVRSGLPSMAWFRKEPALRETQRVEDRHMCDAQTSCPRDDTCCFINRIGKWGCCPLPKAVCCKDGDHCCPSGYTCNEEKTSCTKGLHQIPWFTKKTARVWKSSDELLGHEDVKCDSSTSCPSGSTCCILPTG-QWGCCPLVKAVCCEDHEHCCPQGYICKLELGTCEKASADVSVSLTAVQMPEIQCDTFTRCAHTQSCCRLADSTWACCPYTQAVCCKDMKHCCPMGYKCDPKVQGCTK 718
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 220.32 bits (560), Expect = 8.784e-63 Identity = 145/389 (37.28%), Postives = 195/389 (50.13%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEV----------VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--------DHQAEALEIKISPIL-VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
DG + CCP AVCC D HCCP +CN+ G+CD V + K K+ V +S S V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ +S +S + C D + C G+TCC DG +GCCP AVCC D + CCP CN+ AG+CD + +K + ++ Q P +V C +A + C + TCCK G+ +GCCP+S A CC+D HCCP + CN++ +C + A E +P L + +KC S C TCC + D CCP+P AVCC D HCCP C L +SC K L I
Sbjct: 569 DGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACND-TVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQ-RPSGKVKC-NATHGCPESSTCCKNIAGE-WGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQL 953 HSP 2 Score: 218.779 bits (556), Expect = 2.836e-62 Identity = 139/394 (35.28%), Postives = 201/394 (51.02%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-------VLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKTKKPSLLIKTFQP-----------NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPILVESIKCPNGSL-------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIIN 1306
T DG ++CCP AVCC D HCCP +C++ G+C+ V K V I+ + A + +S + C D C DGS+CC DG + CCP AVCC D HCCP CN++AG+CD V + K K+ + S+ S + P + ++ C G+TCC DG + CCP AVCC D + CCP C++ AG+C DS P + +P +PV+ + + C DG TCCK GD +GCCP A CC D IHCCP CN+ G+CD + ++ ++ P+ + + + P+G + C + TCC + + CCP+ AVCC+D HCCP + C+L++ SC K ++ P N
Sbjct: 479 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGD-WGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVV-IPWYN 870 HSP 3 Score: 206.068 bits (523), Expect = 9.715e-58 Identity = 132/393 (33.59%), Postives = 200/393 (50.89%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCD------SVLKEV----VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKL---KEVVTKMKLKIPAISNL------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT--------------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
T DG ++CCP AVCC D HCCP +C++ G+C+ S +++V + K K+ V S +S V C D + C DG++CC +G++ACCP AVCC D HCCP+ CN++A C+ L + +V + ++ N+ + C D ++ C G+TCC DG + CCP AVCC D + CCP C++ AG+C+ + +K + + T +PV+ I + C DG TCCK G+ +GCCP A CC D IHCCP CN+ G+CD + ++ ++ + PI + + C + + C TCC + + ++CCP P AVCC D HCCP+ C + SC+ +
Sbjct: 306 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTV--SPVSSDIACNDTVACPDGSTCCKDKDGE-WGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDS 694 HSP 4 Score: 205.297 bits (521), Expect = 1.458e-57 Identity = 135/395 (34.18%), Postives = 189/395 (47.85%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVL---KEVVTKIKLK------VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT-FQPNPVNRVICPDAK-----------YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD-----------------HQAEALEIKISPILV-----ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
T +G ++CCP AVCC D HCCP+ +CN+ C+ L + +V + ++ V + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + +S +S I C D + C G+TCC DG +GCCP AVCC D + CCP CN+ AG+CD + + K +P +V A C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + + K+SP+ +++ CP+GS TCC + + CCP P AVCC D HCCP+ C++ SCD
Sbjct: 394 TKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACND-TVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGS------TCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 780 HSP 5 Score: 194.512 bits (493), Expect = 9.255e-54 Identity = 132/367 (35.97%), Postives = 188/367 (51.23%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-------VLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV--VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC---DSKTKKPSLLIKTFQPNP---VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISP------------ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T DG ++CCP AVCC D HCCP +C++ G+C+ V K V I+ + A++ +S V C D C DGS+CC DG++ CCP AVCC D HCCP CN++AG+CD V V K+ + + A S ++C + + C +TCC G +GCCPFS AVCC+D CCP + CNL + +C D + + P P + V C D + CS TCC L + D GCCP+ A CC D+ HCCP+ C++ + +C + L ++I+ ++V+ ++C G C TCC ++ ++ CCP P AVCC D HCCP C
Sbjct: 655 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDSSGSVPWVEKVPVRPIRKQKVANTKVSPVSSDVACNDTVACPDGSTCCKDKDGDWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKC-DEQSSCSADSTCCLL-SKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSC-VKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCG 1017 HSP 6 Score: 192.971 bits (489), Expect = 2.921e-53 Identity = 134/418 (32.06%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS--------------VLKEVVTKIKLKVPASSILS-----------------------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK------TFQPNPVNR----VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVE------------SIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
DG + CCP AVCC DK HCCP++T C++ C S L+ K K P + + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + +S++S + P + ++ C G TCC +G + CCP AVCC D + CCP+ CN+ A C+ L+K T PN + V C D C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + I C + C TCC + + CCP P AVCC D HCCP+ C++ SCD
Sbjct: 189 DGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDT-AACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 604 HSP 7 Score: 182.956 bits (463), Expect = 7.969e-50 Identity = 125/424 (29.48%), Postives = 188/424 (44.34%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV-----LKEVVTKIKLKVPASSIL---------SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-----------------------DSKTKKPSLLIKTFQP-------NPVNRVICPDAKYQ-------------CSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G +SCCP+ CC D HCCP+ C++ TC + L + K +P S + + CPD S C SC +M+ ++ CCP + + CSD +HCCP + C+ + C K K+K+ ++ C +G+S C TCC DG +GCCP AVCC DK+ CCP++TVC++ A C D K K+P T +P N++ A++Q C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + + C + + C TCC + ++CCP P AVCC D HCCP C++ C+ + + P++ P
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLC--------VKRKVKV-----QTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTP 445 HSP 8 Score: 140.969 bits (354), Expect = 1.676e-35 Identity = 107/358 (29.89%), Postives = 155/358 (43.30%), Query Frame = 2
Query: 395 IKLKVPASSILSVVC---PDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----------DSKLKEVVT-----KMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI------------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++L V + + V+C P+ C+ G SCC G F+CCP+ CC D HCCP+ +C++ TC D L + +M +PA S I CPDGSS C +C L+S SYGCCP + + CSD CCP + C+ + C + K V V+C + +C TCC+ G +GCCP A CC DKIHCCP++TVC++ C DH+ + E + + + + C + + C TCC + + ++CCP P AVCC D HCCP+ C + SC+
Sbjct: 2 LRLTVCLAVVTLVICSQCPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPA-SESDITCPDGSS-CPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVK------------VQTVLCGNGTSECPADTTCCQADDG-QWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCE 343 HSP 9 Score: 67.0106 bits (162), Expect = 2.788e-11 Identity = 60/229 (26.20%), Postives = 85/229 (37.12%), Query Frame = 2
Query: 782 CPQNTVCNL-------PAG-------------------------------TCDSKTKKPSLLIKTFQPNP--------VNRVICPDAKYQCSDGQTCCK----LPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP N VC P G TC + T L +T P + + ++ C DG +C L SYGCCP + CSD HCCP + C+ + C + + V+++ C NG S C TCC + + + CCP P AVCC D HCCP +T C + C + + P+ + P
Sbjct: 19 CPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRK---------VKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFP 238
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 217.238 bits (552), Expect = 3.213e-62 Identity = 138/387 (35.66%), Postives = 196/387 (50.65%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVL---KEVVTKIKLK------VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--------DHQAEALEIKISPIL-VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
T +G ++CCP AVCC D HCCP+ +CN+ C+ L + +V + ++ V + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + S+ S + P + ++ C G+TCC DG + CCP AVCC D + CCP CN+ AG+CD + +K + ++ Q P +V C +A + C + TCCK G+ +GCCP+S A CC+D HCCP + CN++ +C + A E +P L + +KC S C TCC + D CCP+P AVCC D HCCP C L +SC K L I
Sbjct: 394 TKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQ-RPSGKVKC-NATHGCPESSTCCKNIAGE-WGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQL 777 HSP 2 Score: 204.142 bits (518), Expect = 1.712e-57 Identity = 133/392 (33.93%), Postives = 201/392 (51.28%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCD------SVLKEV----VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKL---KEVVTKMKLKIPAISNL------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT-FQPNPVNRVICPDAK-----------YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPILVESIKCPNGSL-------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIIN 1306
T DG ++CCP AVCC D HCCP +C++ G+C+ S +++V + K K+ V S +S V C D + C DG++CC +G++ACCP AVCC D HCCP+ CN++A C+ L + +V + ++ N+ + C D ++ C G+TCC DG + CCP AVCC D + CCP C++ AG+C+ + + K +P +V A C DG TCCK G + CCP A CC D IHCCP CN+ G+CD + ++ ++ P+ + + + P+G + C + TCC + + CCP+ AVCC+D HCCP + C+L++ SC K ++ P N
Sbjct: 306 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVV-IPWYN 694 HSP 3 Score: 196.438 bits (498), Expect = 9.288e-55 Identity = 133/367 (36.24%), Postives = 187/367 (50.95%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEV----------VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV--VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC---DSKTKKPSLLIKTFQPNP---VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISP------------ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T DG ++CCP AVCC D HCCP +C++ G+C+ V + K K+ V +S S V C D + C DGS+CC DG +ACCP AVCC D HCCP CN++AG+CD V V K+ + + A S ++C + + C +TCC G +GCCPFS AVCC+D CCP + CNL + +C D + + P P + V C D + CS TCC L + D GCCP+ A CC D+ HCCP+ C++ + +C + L ++I+ ++V+ ++C G C TCC ++ ++ CCP P AVCC D HCCP C
Sbjct: 479 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKC-DEQSSCSADSTCCLL-SKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSC-VKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCG 841 HSP 4 Score: 194.512 bits (493), Expect = 5.183e-54 Identity = 133/418 (31.82%), Postives = 189/418 (45.22%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS--------------VLKEVVTKIKLKVPASSILS-----------------------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK------TFQPNPVNR----VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
DG + CCP AVCC DK HCCP++T C++ C S L+ K K P + + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + +S++S + P + ++ C G TCC +G + CCP AVCC D + CCP+ CN+ A C+ L+K T PN + V C D C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + + C + + C TCC + + ++CCP P AVCC D HCCP+ C++ SCD
Sbjct: 189 DGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDT-AACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 604 HSP 5 Score: 182.956 bits (463), Expect = 5.464e-50 Identity = 125/424 (29.48%), Postives = 188/424 (44.34%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV-----LKEVVTKIKLKVPASSIL---------SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-----------------------DSKTKKPSLLIKTFQP-------NPVNRVICPDAKYQ-------------CSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G +SCCP+ CC D HCCP+ C++ TC + L + K +P S + + CPD S C SC +M+ ++ CCP + + CSD +HCCP + C+ + C K K+K+ ++ C +G+S C TCC DG +GCCP AVCC DK+ CCP++TVC++ A C D K K+P T +P N++ A++Q C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + + C + + C TCC + ++CCP P AVCC D HCCP C++ C+ + + P++ P
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLC--------VKRKVKV-----QTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTP 445 HSP 6 Score: 141.354 bits (355), Expect = 1.177e-35 Identity = 107/358 (29.89%), Postives = 155/358 (43.30%), Query Frame = 2
Query: 395 IKLKVPASSILSVVC---PDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----------DSKLKEVVT-----KMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI------------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++L V + + V+C P+ C+ G SCC G F+CCP+ CC D HCCP+ +C++ TC D L + +M +PA S I CPDGSS C +C L+S SYGCCP + + CSD CCP + C+ + C + K V V+C + +C TCC+ G +GCCP A CC DKIHCCP++TVC++ C DH+ + E + + + + C + + C TCC + + ++CCP P AVCC D HCCP+ C + SC+
Sbjct: 2 LRLTVCLAVVTLVICSQCPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPA-SESDITCPDGSS-CPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVK------------VQTVLCGNGTSECPADTTCCQADDG-QWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCE 343 HSP 7 Score: 66.6254 bits (161), Expect = 2.735e-11 Identity = 60/229 (26.20%), Postives = 85/229 (37.12%), Query Frame = 2
Query: 782 CPQNTVCNL-------PAG-------------------------------TCDSKTKKPSLLIKTFQPNP--------VNRVICPDAKYQCSDGQTCCK----LPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP N VC P G TC + T L +T P + + ++ C DG +C L SYGCCP + CSD HCCP + C+ + C + + V+++ C NG S C TCC + + + CCP P AVCC D HCCP +T C + C + + P+ + P
Sbjct: 19 CPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRK---------VKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFP 238
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 217.238 bits (552), Expect = 3.516e-62 Identity = 141/388 (36.34%), Postives = 197/388 (50.77%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVL---KEVVTKIKLK------VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--------DHQAEALEIKISPIL-VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
T +G ++CCP AVCC D HCCP+ +CN+ C+ L + +V + ++ V + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + +S +S I C D + C G+TCC DG +GCCP AVCC D + CCP CN+ AG+CD + +K + ++ Q P +V C +A + C + TCCK G+ +GCCP+S A CC+D HCCP + CN++ +C + A E +P L + +KC S C TCC + D CCP+P AVCC D HCCP C L +SC K L I
Sbjct: 394 TKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACND-TVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQ-RPSGKVKC-NATHGCPESSTCCKNIAGE-WGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKCDEQSSCSADSTCCLLSKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSCVKTTRLYVEITQL 777 HSP 2 Score: 209.149 bits (531), Expect = 2.573e-59 Identity = 135/394 (34.26%), Postives = 207/394 (52.54%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCD------SVLKEV----VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKL---KEVVTKMKLKIPAISNL------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT--------------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPILVESIKCPNGSL-------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIIN 1306
T DG ++CCP AVCC D HCCP +C++ G+C+ S +++V + K K+ V S +S V C D + C DG++CC +G++ACCP AVCC D HCCP+ CN++A C+ L + +V + ++ N+ + C D ++ C G+TCC DG + CCP AVCC D + CCP C++ AG+C+ + +K + + T +PV+ I + C DG TCCK G+ +GCCP A CC D IHCCP CN+ G+CD + ++ ++ P+ + + + P+G + C + TCC + + CCP+ AVCC+D HCCP + C+L++ SC K ++ P N
Sbjct: 306 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTV--SPVSSDIACNDTVACPDGSTCCKDKDGE-WGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKCNATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVV-IPWYN 694 HSP 3 Score: 193.741 bits (491), Expect = 1.010e-53 Identity = 131/367 (35.69%), Postives = 186/367 (50.68%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-------VLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV--VTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC---DSKTKKPSLLIKTFQPNP---VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISP------------ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T DG ++CCP AVCC D HCCP +C++ G+C+ V K V I+ + A + +S + C D C DGS+CC DG + CCP AVCC D HCCP CN++AG+CD V V K+ + + A S ++C + + C +TCC G +GCCPFS AVCC+D CCP + CNL + +C D + + P P + V C D + CS TCC L + D GCCP+ A CC D+ HCCP+ C++ + +C + L ++I+ ++V+ ++C G C TCC ++ ++ CCP P AVCC D HCCP C
Sbjct: 479 TKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWVEKVPVHLRAGQRPSGKVKC-NATHGCPESSTCCKNIAGEWGCCPFSQAVCCTDGEHCCPAHYKCNLSSVSCIKGDVVIPWYNKIAAESTPAPKLDLGVVKC-DEQSSCSADSTCCLL-SKDETGCCPFPEAVCCPDQKHCCPEGYRCDLRRRSC-VKTTRLYVEITQLTHIRSNKPQPSVVVKDVQCGGGFSCHDGETCCLTSQTTWGCCPSPKAVCCDDMQHCCPAGYKCG 841 HSP 4 Score: 192.971 bits (489), Expect = 1.601e-53 Identity = 134/418 (32.06%), Postives = 186/418 (44.50%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS--------------VLKEVVTKIKLKVPASSILS-----------------------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK------TFQPNPVNR----VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVE------------SIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
DG + CCP AVCC DK HCCP++T C++ C S L+ K K P + + V C D + C DGS+CC DG +ACCP AVCC D HCCP C+++AG+C+ V + K K+ + +S++S + P + ++ C G TCC +G + CCP AVCC D + CCP+ CN+ A C+ L+K T PN + V C D C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + I C + C TCC + + CCP P AVCC D HCCP+ C++ SCD
Sbjct: 189 DGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDT-AACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVPWVEKVPVRPIRKQKVAVTTVSPVSSDIACNDTVACPDGSTCCKDKDGEWGCCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 604 HSP 5 Score: 182.956 bits (463), Expect = 5.105e-50 Identity = 125/424 (29.48%), Postives = 188/424 (44.34%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV-----LKEVVTKIKLKVPASSIL---------SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-----------------------DSKTKKPSLLIKTFQP-------NPVNRVICPDAKYQ-------------CSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G +SCCP+ CC D HCCP+ C++ TC + L + K +P S + + CPD S C SC +M+ ++ CCP + + CSD +HCCP + C+ + C K K+K+ ++ C +G+S C TCC DG +GCCP AVCC DK+ CCP++TVC++ A C D K K+P T +P N++ A++Q C DG TCCK G + CCP A CC D IHCCP C++ G+C+ + ++ ++ + PI + + C + + C TCC + ++CCP P AVCC D HCCP C++ C+ + + P++ P
Sbjct: 37 GGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLC--------VKRKVKV-----QTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTP 445 HSP 6 Score: 141.354 bits (355), Expect = 1.082e-35 Identity = 107/358 (29.89%), Postives = 155/358 (43.30%), Query Frame = 2
Query: 395 IKLKVPASSILSVVC---PDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----------DSKLKEVVT-----KMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI------------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++L V + + V+C P+ C+ G SCC G F+CCP+ CC D HCCP+ +C++ TC D L + +M +PA S I CPDGSS C +C L+S SYGCCP + + CSD CCP + C+ + C + K V V+C + +C TCC+ G +GCCP A CC DKIHCCP++TVC++ C DH+ + E + + + + C + + C TCC + + ++CCP P AVCC D HCCP+ C + SC+
Sbjct: 2 LRLTVCLAVVTLVICSQCPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPA-SESDITCPDGSS-CPAEFSCLLMST-SYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRKVK------------VQTVLCGNGTSECPADTTCCQADDG-QWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFPARLRAEWEDHKQKRPETQRTTARPTGTTSTTTAANQMTTLPAEHQGVSSDVACNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCDVAAGSCE 343 HSP 7 Score: 67.0106 bits (162), Expect = 2.664e-11 Identity = 60/229 (26.20%), Postives = 85/229 (37.12%), Query Frame = 2
Query: 782 CPQNTVCNL-------PAG-------------------------------TCDSKTKKPSLLIKTFQPNP--------VNRVICPDAKYQCSDGQTCCK----LPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP N VC P G TC + T L +T P + + ++ C DG +C L SYGCCP + CSD HCCP + C+ + C + + V+++ C NG S C TCC + + + CCP P AVCC D HCCP +T C + C + + P+ + P
Sbjct: 19 CPNNEVCEAGQSCCQDPTGGFSCCPFHHGECCEDHLHCCPEGMLCSVKDLTCTNATHTQPLADRTLAKKPDLPKSFRMIFSMPASESDITCPDGSSCPAEFSCLLMSTSYGCCPVAQGLACSDGKHCCPNDHECSTDSSLCVKRK---------VKVQTVLCGNGTSECPADTTCCQADDGQWGCCPMPKAVCCDDKIHCCPEDTVCDVKALKCVSSTNQELPMWDKFP 238
BLAST of Granulin precursor vs. Ensembl Zebrafish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 138.272 bits (347), Expect = 2.724e-37 Identity = 80/211 (37.91%), Postives = 112/211 (53.08%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVL---KEVVTKIKLK------VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCD 826
T +G ++CCP AVCC D HCCP+ +CN+ C+ L + +V + ++ V + V C D + C DGS+CC DG +ACCP AVCC D HCCP CN++AG+CD V + K K+ + S+ S + P + ++ C G+TCC DG + CCP AVCC D + CCP CN+ AG+CD
Sbjct: 60 TKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 270 HSP 2 Score: 137.887 bits (346), Expect = 4.425e-37 Identity = 89/253 (35.18%), Postives = 126/253 (49.80%), Query Frame = 2
Query: 386 VTKIKLKVPASSILS--VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKL---KEVVTKMKLKIPAISNL------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT-FQPNPVNRVICPDAK-----------YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL 1075
+ K K+ V S +S V C D + C DG++CC +G++ACCP AVCC D HCCP+ CN++A C+ L + +V + ++ N+ + C D ++ C G+TCC DG + CCP AVCC D + CCP CN+ AG+CD + + K +P +V A C DG TCCK G + CCP A CC D IHCCP CN+ G+CD + ++
Sbjct: 26 IRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCND-TAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSV 276 HSP 3 Score: 131.724 bits (330), Expect = 9.016e-35 Identity = 87/271 (32.10%), Postives = 130/271 (47.97%), Query Frame = 2
Query: 572 NLSAGTCDSKLKEV----------VTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK------TFQPNPVNR----VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIK------------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
+++AG+C+ V + K K+ + +S++S + P + ++ C G TCC +G + CCP AVCC D + CCP+ CN+ A C+ L+K T PN + V C D C DG TCCK G + CCP A CC D IHCCP CN+ G+CD + ++ ++ + PI + + C + + C TCC + + ++CCP P AVCC D HCCP+ C++ SCD
Sbjct: 2 DVAAGSCEDPSGSVSWVEKVPVRPIRKQKVAVTQVSSVSSDVPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTPVQSTTTPNVIGTQKSDVPCNDT-AACPDGSTCCKTKDG-GWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCDDPSGSVPWMDKVPVRPIKKQKVAVTQASSASSDVPCNDTAACPDGSTCCKTKDGGWACCPLPEAVCCEDFIHCCPHGKKCNVAAGSCD 270 HSP 4 Score: 51.6026 bits (122), Expect = 6.388e-7 Identity = 22/69 (31.88%), Postives = 35/69 (50.72%), Query Frame = 2
Query: 1109 IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
+ C + + C TCC + ++CCP P AVCC D HCCP C++ C+ + + P++ P
Sbjct: 43 VPCNDTAACADGTTCCKTKEGDWACCPLPEAVCCEDFVHCCPKGKKCNIAAMKCEDPLCTEEPLVKQTP 111
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 202.986 bits (515), Expect = 1.973e-57 Identity = 132/336 (39.29%), Postives = 168/336 (50.00%), Query Frame = 2
Query: 266 SYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL----KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSI-ECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVES--IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S+ CCP AVCC D HCCP N+EC++ +G C S + KL K V C D + C DGS+CC D + CC SAVCCSD HCCP T C+L C S+ E + ++PAI S + P ++T K TCC L G +GCCP AVCC+D L CCP+ C+ G C KT R + D Y C DGQTCC+L +GD +GCCP + A CC D HCCP C+ + + L K + E+ +KC + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 142 SWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESANQVPCDATTKK--ETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 470 HSP 2 Score: 184.496 bits (467), Expect = 9.085e-51 Identity = 132/362 (36.46%), Postives = 176/362 (48.62%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCD---DGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI----LLKFPIINF 1309
D +Y CC SAVCCSD HCCP T C+L C S E + ++PA S +Q CD +CC + G + CCP AVCC+D HCCP+ C S G C SK++ + K PA+++ + +EC D S C G TCC L+ G +GCCP + AVCC D CCP C+ G C L KT + + D + C+DGQTCC+L +GD +GCCP + A CC D HCCP C+ ++ + K +P L + ++C + C TCC A+ + CCP AVCCSDH HCCP +C L K P + F
Sbjct: 220 DHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESA---NQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGEC-SKMEHSIPWFT-KTPALTHEARDVECDDMYS-CPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGSQCMIGEHSIPWFSK-TPALKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPF 567 HSP 3 Score: 181.03 bits (458), Expect = 1.699e-49 Identity = 125/345 (36.23%), Postives = 176/345 (51.01%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMAD-GNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL----KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T +G +CCP + C D HCC + C+ + C +PAS+ +VVCPD +S+C ++CC+M+D ++ CCP AVCC D HCCP N+ C++ G C S + KL K + C DG + C G+TCC D +YGCC +AVCCSD L CCP T C+L C S+T + LL + + N+V C DA + +TCC+L +G +GCCP A CC+D +HCCP+ C ++G C ++ +P L ++C + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 74 TPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDG-TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DAT---TKKETCCRLVSG-KWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 396 HSP 4 Score: 173.711 bits (439), Expect = 5.864e-47 Identity = 113/319 (35.42%), Postives = 154/319 (48.28%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCP 1189
G + CCP AVCC+D HCCP+ C+ +G C + + K V C D C DG +CC +A G++ CCP + AVCC D +HCCP C S G C + E + K PA+ + ++C D + +C G TCC L+ G +GCCP + AVCC D CCP C+ S+ I F P + D + Y C+DGQTCC+L +GD +GCCP + A CCSD HCCPQ C+ +GTC ++ ++ P C + C TCCP + CCP
Sbjct: 295 GKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPLFSKTPALRQETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS------GSQCMIGEHSIPWFSKTPALKQKAKDVQCDDMYSCADGQTCCRLASGD-WGCCPIAQAVCCSDHWHCCPQGFTCDA-RGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCP 598 HSP 5 Score: 147.132 bits (370), Expect = 1.345e-37 Identity = 113/360 (31.39%), Postives = 153/360 (42.50%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDK--IHCCPQNTVCNINKGTCDH-------QAEALEIKISPILVESIK-----CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G + CCP + AVCC D +HCCP C+ G C + K V C D C DG +CC +A G++ CCP + AVCC D +HCCP C+ S + E K PA+ + ++C D S C G TCC L+ G +GCCP + AVCCSD CCPQ C+ + P L P IC D + C G TCC G + CCP + + I V ++ C Q EA+E S V+ ++ C + C TCC ++CC Y VCC D HCCP C + SC ++ ++
Sbjct: 369 GDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGS----QCMIGEHSIPWFSKTPALKQKAKDVQCDDMYS-CADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSIC-DDTHTCPSGTTCCP-GEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRW 719 HSP 6 Score: 146.747 bits (369), Expect = 1.740e-37 Identity = 101/276 (36.59%), Postives = 132/276 (47.83%), Query Frame = 2
Query: 449 SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK-----TFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA--LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S C D SC +G ACCP + C D HCC + C+ C IPA + ++ CPDG S C T TCC++SD S+GCCP AVCC D + CCP N+ C++ G C S + K + + RV C D + C DG TCC+ +YGCC +A CCSD +HCCP T C++ C Q L ++ I ES +K TCC + + CCP AVCC+DH HCCP +CS
Sbjct: 63 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTF-CPDGSTCCEQ-VDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS 322 HSP 7 Score: 113.235 bits (282), Expect = 3.614e-26 Identity = 90/316 (28.48%), Postives = 134/316 (42.41%), Query Frame = 2
Query: 401 LKVPASSILSVVCPDQSQCDDGSSCCVM-ADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-DHQAEALEIKISPILVES-------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFPIIN 1306
L + S++ + +CPD S C + S CC + + CCP + EVV++ I + ++ S CPD S C +G CCP + C D CC + C+ C + + + V+CPD + +C TCC + S+GCCP A CC D +HCCP N+ C++ +G C +Q + P V+S + C +G+ C TCC + +Y CC +AVCCSDH HCCP T+C L K C + +L + P I
Sbjct: 7 LLLVVSTVSATLCPDGSTCGEKSLCCELPGKKGYGCCPAA------------------------------EVVSRSLPMILSQTSCS-GCPDEYS-------CVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPASNQ-------------SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIR 271
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 203.371 bits (516), Expect = 1.991e-57 Identity = 132/336 (39.29%), Postives = 168/336 (50.00%), Query Frame = 2
Query: 266 SYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL----KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSI-ECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVES--IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S+ CCP AVCC D HCCP N+EC++ +G C S + KL K V C D + C DGS+CC D + CC SAVCCSD HCCP T C+L C S+ E + ++PAI S + P ++T K TCC L G +GCCP AVCC+D L CCP+ C+ G C KT R + D Y C DGQTCC+L +GD +GCCP + A CC D HCCP C+ + + L K + E+ +KC + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 162 SWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESANQVPCDATTKK--ETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 490 HSP 2 Score: 184.882 bits (468), Expect = 9.260e-51 Identity = 132/362 (36.46%), Postives = 176/362 (48.62%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCD---DGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI----LLKFPIINF 1309
D +Y CC SAVCCSD HCCP T C+L C S E + ++PA S +Q CD +CC + G + CCP AVCC+D HCCP+ C S G C SK++ + K PA+++ + +EC D S C G TCC L+ G +GCCP + AVCC D CCP C+ G C L KT + + D + C+DGQTCC+L +GD +GCCP + A CC D HCCP C+ ++ + K +P L + ++C + C TCC A+ + CCP AVCCSDH HCCP +C L K P + F
Sbjct: 240 DHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESA---NQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGEC-SKMEHSIPWFT-KTPALTHEARDVECDDMYS-CPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGSQCMIGEHSIPWFSK-TPALKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPF 587 HSP 3 Score: 181.415 bits (459), Expect = 1.522e-49 Identity = 125/345 (36.23%), Postives = 176/345 (51.01%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMAD-GNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL----KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T +G +CCP + C D HCC + C+ + C +PAS+ +VVCPD +S+C ++CC+M+D ++ CCP AVCC D HCCP N+ C++ G C S + KL K + C DG + C G+TCC D +YGCC +AVCCSD L CCP T C+L C S+T + LL + + N+V C DA + +TCC+L +G +GCCP A CC+D +HCCP+ C ++G C ++ +P L ++C + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 94 TPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDG-TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DAT---TKKETCCRLVSG-KWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 416 HSP 4 Score: 174.096 bits (440), Expect = 6.030e-47 Identity = 113/319 (35.42%), Postives = 154/319 (48.28%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCP 1189
G + CCP AVCC+D HCCP+ C+ +G C + + K V C D C DG +CC +A G++ CCP + AVCC D +HCCP C S G C + E + K PA+ + ++C D + +C G TCC L+ G +GCCP + AVCC D CCP C+ S+ I F P + D + Y C+DGQTCC+L +GD +GCCP + A CCSD HCCPQ C+ +GTC ++ ++ P C + C TCCP + CCP
Sbjct: 315 GKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPLFSKTPALRQETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS------GSQCMIGEHSIPWFSKTPALKQKAKDVQCDDMYSCADGQTCCRLASGD-WGCCPIAQAVCCSDHWHCCPQGFTCDA-RGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCP 618 HSP 5 Score: 147.132 bits (370), Expect = 1.159e-37 Identity = 113/355 (31.83%), Postives = 151/355 (42.54%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDK--IHCCPQNTVCNINKGTCDH-------QAEALEIKISPILVESIK-----CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
G + CCP + AVCC D +HCCP C+ G C + K V C D C DG +CC +A G++ CCP + AVCC D +HCCP C+ S + E K PA+ + ++C D S C G TCC L+ G +GCCP + AVCCSD CCPQ C+ + P L P IC D + C G TCC G + CCP + + I V ++ C Q EA+E S V+ ++ C + C TCC ++CC Y VCC D HCCP C + SC ++
Sbjct: 389 GDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPLFSKTPALRQETKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGS----QCMIGEHSIPWFSKTPALKQKAKDVQCDDMYS-CADGQTCCRLASGDWGCCPIAQAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSICDDT-HTCPSGTTCCP-GEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRS 734 HSP 6 Score: 147.132 bits (370), Expect = 1.388e-37 Identity = 101/276 (36.59%), Postives = 132/276 (47.83%), Query Frame = 2
Query: 449 SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK-----TFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA--LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S C D SC +G ACCP + C D HCC + C+ C IPA + ++ CPDG S C T TCC++SD S+GCCP AVCC D + CCP N+ C++ G C S + K + + RV C D + C DG TCC+ +YGCC +A CCSD +HCCP T C++ C Q L ++ I ES +K TCC + + CCP AVCC+DH HCCP +CS
Sbjct: 83 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTF-CPDGSTCCEQ-VDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPCDATTKKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS 342 HSP 7 Score: 113.235 bits (282), Expect = 3.281e-26 Identity = 90/316 (28.48%), Postives = 134/316 (42.41%), Query Frame = 2
Query: 401 LKVPASSILSVVCPDQSQCDDGSSCCVM-ADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-DHQAEALEIKISPILVES-------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFPIIN 1306
L + S++ + +CPD S C + S CC + + CCP + EVV++ I + ++ S CPD S T +G CCP + C D CC + C+ C + + + V+CPD + +C TCC + S+GCCP A CC D +HCCP N+ C++ +G C +Q + P V+S + C +G+ C TCC + +Y CC +AVCCSDH HCCP T+C L K C + +L + P I
Sbjct: 27 LLLVVSTVSATLCPDGSTCGEKSLCCELPGKKGYGCCPAA------------------------------EVVSRSLPMILSQTSCS-GCPDEYSCVNT-------PEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPASNQ-------------SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIR 291
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 193.741 bits (491), Expect = 2.109e-53 Identity = 128/347 (36.89%), Postives = 178/347 (51.30%), Query Frame = 2
Query: 266 SYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEV--VTKIKLKVPASSI---------LSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSI-ECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPILVES---IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S+ CCP AVCC D HCCP N+EC++ +G C S + ++K+ +V + + V C D + C DGS+CC D + CC SAVCCSD HCCP T C+L C S+ E + ++PAI S + P D +++C TCC LS +GCCP++ AVCC D + CCP C+ G+C + KT Q RV C D C + +TCC+L +G +GCCP A CC+D +HCCP+ C ++G C ++ +P L ++C + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 142 SWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCS--GGSCVLAEHSIPWMRKTLAQGLKTTRVQCDDTA-SCPEKETCCRLVSG-KWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 480 HSP 2 Score: 190.274 bits (482), Expect = 3.139e-52 Identity = 130/352 (36.93%), Postives = 183/352 (51.99%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMAD-GNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC---------DSKLKEVVTKMKLKIPAISN--LSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIKISPIL-VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
T +G +CCP + C D HCC + C+ + C +PAS+ +VVCPD +S+C ++CC+M+D ++ CCP AVCC D HCCP N+ C++ G C SKL V LK+ + + + C DG + C G+TCC D +YGCC +AVCCSD L CCP T C+L C S+T + LL + + N+V C DA C D TCC L + + +GCCPY+ A CC D IHCCP C + G+C +H + ++ L ++C + + C +K TCC + + CCP AVCC+DH HCCP +CS
Sbjct: 74 TPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDG-TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DATTSCPDKNTCCHL-SSEKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSIPWMRKTLAQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS 406 HSP 3 Score: 168.703 bits (426), Expect = 7.796e-45 Identity = 127/410 (30.98%), Postives = 171/410 (41.71%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-EIKISPILVES---IKCPNGSLCGQKGTCCPSANDSYSCCPYPN-----------------------------------------------AVCCSDHSHCCPNNTSCSLNTKSCDKNI----LLKFPIINF 1309
G + CCP AVCC+D HCCP+ C+ +G C + + K V C D C DG +CC +A G++ CCP + AVCC D +HCCP C S G C + E + K PA+ + ++C D + +C G TCC L+ G +GCCP + AVCC D CCP C+ S+ I F P + D + Y C+DGQTCC+L +GD +GCCP + A CC D HCCP C + G C ++ +P L + +KC + C TCC + + CCP AVCCSDH HCCP +C L K P + F
Sbjct: 379 GKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPALTHEARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPLFSKTPALRQETKYVKC-DDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS------GSQCMIGEHSIPWFSKTPALKQKAKDVQCDDMYSCADGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTC--SGGQCQKGEHSIPWYSKTPALKQEGNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKCCQTHTPLLPLQKCCPSLPILALATCHVQDSVQDSCTRLQSTHWCRTAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPF 772 HSP 4 Score: 152.14 bits (383), Expect = 3.284e-39 Identity = 104/287 (36.24%), Postives = 138/287 (48.08%), Query Frame = 2
Query: 449 SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ-AEALEIKISPILVES----IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S C D SC +G ACCP + C D HCC + C+ C IPA + ++ CPDG S C T TCC++SD S+GCCP AVCC D + CCP N+ C++ G C S + K L + + RV C D + C DG TCC+ +YGCC +A CCSD +HCCP T C++ C Q E + P + E + C + C K TCC +++ + CCPY AVCC DH HCCP+ +CS
Sbjct: 63 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLG-DEERRVPCLDGTF-CPDGSTCCEQ-VDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCS 332 HSP 5 Score: 123.635 bits (309), Expect = 1.508e-29 Identity = 116/409 (28.36%), Postives = 156/409 (38.14%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPA--SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSN-----------------------------------------------AVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDK--IHCCPQNTVCNINKGTCDH-------QAEALEIKISPILVESIK-----CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G + CCP + AVCC D +HCCP C+ ++ ++ E K PA V C D C DG +CC +A G++ CCP + AVCC D +HCCP C S G C + E K PA+ ++C D S CK G TCC + G +GCCP AVCCSD CCPQ C+ + P L P IC D + C G TCC G + CCP + + I V ++ C Q EA+E S V+ ++ C + C TCC ++CC Y VCC D HCCP C + SC ++ ++
Sbjct: 527 GDWGCCPIAQAVCCDDHEHCCPPGYTCSGSQ----CMIGEHSIPWFSKTPALKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPWYSKTPALKQEGNIVKC-DDSFACKDGYTCCRMVSGVWGCCPIEKCCQTHTPLLPLQKCCPSLPILALATCHVQDSVQDSCTRLQSTHWCRTAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPFDGTHSICDDT-HTCPSGTTCCP-GEGGGWRCCPVEEVSKMVKRTVILLATACIVTLVSFSFCPDIIIYYALQKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRW 924 HSP 6 Score: 112.849 bits (281), Expect = 5.362e-26 Identity = 90/323 (27.86%), Postives = 135/323 (41.80%), Query Frame = 2
Query: 401 LKVPASSILSVVCPDQSQCDDGSSCCVM-ADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----DH-----------QAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFPIIN 1306
L + S++ + +CPD S C + S CC + + CCP + EVV++ I + ++ S CPD S T +G CCP + C D CC + C+ C + + + V+CPD + +C TCC + S+GCCP A CC D +HCCP N+ C++ +G C DH ++E L++ + C +G+ C TCC + +Y CC +AVCCSDH HCCP T+C L K C + +L + P I
Sbjct: 7 LLLVVSTVSATLCPDGSTCGEKSLCCELPGKKGYGCCPAA------------------------------EVVSRSLPMILSQTSCS-GCPDEYSCVNT-------PEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPASNQ-------------SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIR 278
BLAST of Granulin precursor vs. Ensembl Xenopus
Match: trappc11 (trafficking protein particle complex 11 [Source:Xenbase;Acc:XB-GENE-5931148]) HSP 1 Score: 190.66 bits (483), Expect = 1.689e-52 Identity = 134/374 (35.83%), Postives = 192/374 (51.34%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMAD-GNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC---------DSKLKEVVTKMKLKIPAISN--LSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIKISPIL-VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF---PIINF 1309
T +G +CCP + C D HCC + C+ + C +PAS+ +VVCPD +S+C ++CC+M+D ++ CCP AVCC D HCCP N+ C++ G C SKL V LK+ + + + C DG + C G+TCC D +YGCC +AVCCSD L CCP T C+L C S+T + LL + + N+V C DA C D TCC L + + +GCCPY+ A CC D IHCCP C + G+C +H + ++ L ++C + + C +K TCC + + CCP AVCC+DH HCCP +CS S ++ + F P++ F
Sbjct: 74 TPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDG-TFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPC-DATTSCPDKNTCCHL-SSEKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSIPWMRKTLAQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCSQGECSKMEHSIPWFTKTPMVFF 428 HSP 2 Score: 188.348 bits (477), Expect = 1.426e-51 Identity = 127/360 (35.28%), Postives = 177/360 (49.17%), Query Frame = 2
Query: 266 SYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEV--VTKIKLKVPASSI---------LSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSI-ECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILV-----------------ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S+ CCP AVCC D HCCP N+EC++ +G C S + ++K+ +V + + V C D + C DGS+CC D + CC SAVCCSD HCCP T C+L C S+ E + ++PAI S + P D +++C TCC LS +GCCP++ AVCC D + CCP C+ G+C + KT Q RV C D C + +TCC+L +G +GCCP A CC+D +HCCP+ C ++G C ++ +V ++C + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 142 SWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGE--GPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCS--GGSCVLAEHSIPWMRKTLAQGLKTTRVQCDDTA-SCPEKETCCRLVSG-KWGCCPVVKAVCCNDHLHCCPEGYTC--SQGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 493 HSP 3 Score: 176.792 bits (447), Expect = 1.371e-47 Identity = 129/378 (34.13%), Postives = 168/378 (44.44%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL-KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK-------TKKPSLLIKTFQPN------PVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCP---------------------------QNTVCNINKGTCDHQAEALEIKISPIL-----VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
D +Y CC SAVCCSD HCCP T C+L C S E ++ + S V C + C D ++CC ++ + CCPY+ AVCC D HCCP C S G+C + K + ++C D +++C TCC L G +GCCP AVCC+D L CCP+ C+ G C TK P + + V R + D Y C DGQTCC+L +GD +GCCP + A CC D HCCP Q T C G D L + SP + + +KC + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 226 VDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTC--SGGSCVLAEHSIPWMRKTLAQGLKTTRVQC-DDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVECDDMYSCPDGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGD-DEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 596 HSP 4 Score: 172.555 bits (436), Expect = 3.316e-46 Identity = 129/374 (34.49%), Postives = 174/374 (46.52%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVV---TKMKLKI----------PAIS-NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK-------TKKPSLLIKTF---------QPNPV----NRVICP---------DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
+ CCPY+ AVCC D HCCP C+ G+C + K V C D + C + +CC + G + CCP AVCC+D HCCP+ C S G C SK++ + TK + P +S +EC D S C G TCC L+ G +GCCP + AVCC D CCP C+ G C +K P+L +T P P+ + ICP D + C+DGQTCC+L +GD +GCCP + A CC D HCCP C+ ++ + K +P L + ++C + C TCC A+ + CCP AVCC DH HCCP +CS
Sbjct: 307 WGCCPYAQAVCCDDHIHCCPSGFTCS--GGSCVLAEHSIPWMRKTLAQGLKTTRVQCDDTASCPEKETCCRLVSGKWGCCPVVKAVCCNDHLHCCPEGYTC--SQGEC-SKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVECDDMYS-CPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGSQCMIGEHSIPWFSK-TPALKQKAKDVQCDDMYSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS 670 HSP 5 Score: 168.703 bits (426), Expect = 6.942e-45 Identity = 130/416 (31.25%), Postives = 175/416 (42.07%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKV------------PASSILS-VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS---------------------IECP---------DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCN-------------INKGTCDHQAEALEIKISPILVES---IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI----LLKFPIINF 1309
G + CCP AVCC+D HCCP+ C+ +G C + + K + P S+ V C D C DG +CC +A G++ CCP + AVCC D +HCCP C S G C + E + K PA+ + CP D + +C G TCC L+ G +GCCP + AVCC D CCP C+ S+ I F P + D + Y C+DGQTCC+L +GD +GCCP + A CC D HCCP C+ +K Q + +P+ V + +KC + C TCC + + CCP AVCCSDH HCCP +C L K P + F
Sbjct: 379 GKWGCCPVVKAVCCNDHLHCCPEGYTCS--QGECSKMEHSIPWFTKTPMVFFLSLSLLSILPVVSVARDVECDDMYSCPDGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTC--SGGQC--QKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS------GSQCMIGEHSIPWFSKTPALKQKAKDVQCDDMYSCADGQTCCRLASGD-WGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPWYSKTPALKQEGKCNLANTPVNVFAGNIVKCDDSFACKDGYTCCRMVSGVWGCCPIEKAVCCSDHWHCCPQGFTCDARGTCVLGQFSIPWLTKVPALPF 781 HSP 6 Score: 153.68 bits (387), Expect = 1.192e-39 Identity = 128/441 (29.02%), Postives = 174/441 (39.46%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCP---------------------------QNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK-------TKKPSLLIK---TFQPNPVN----RVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-------------------------------------------------DHQAEALEIKISPILVESIK-----CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G + CCP + AVCC D +HCCP Q T C G + + P S V C D C DG +CC +A G++ CCP + AVCC D +HCCP C+ S + E K PA+ + ++C D S C G TCC L+ G +GCCP + AVCC D CCP C+ G C +K P+L + PVN ++ D + C DG TCC++ +G +GCCP A CCSD HCCPQ C+ +GTC + EA+E S V+ ++ C + C TCC ++CC Y VCC D HCCP C + SC ++ ++
Sbjct: 466 GDWGCCPIAQAVCCDDHEHCCPPGYTCSGGQCQKGEHSIPLFSKTPALRQETACARRCGDDEYPFPLCLLFSPFICPFSVAKYVKCDDTHSCADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCSGS----QCMIGEHSIPWFSKTPALKQKAKDVQCDDMYS-CADGQTCCRLASGDWGCCPIAQAVCCDDHEHCCPPGYTCS--GGQCQKGEHSIPWYSKTPALKQEGKCNLANTPVNVFAGNIVKCDDSFACKDGYTCCRMVSG-VWGCCPIEKAVCCSDHWHCCPQGFTCDA-RGTCVLGQFSIPWLTKVPALPFDGTHSICDDTHTCPSGTTCCPGEGGGWRCCPVEEKEAIETSPSDTSVQGLRLDYVWCDSQYACFDGQTCCRGLGGVWNCCVYTQGVCCPDMVHCCPYGYVCLNHGTSCSRSGSPRW 897 HSP 7 Score: 151.754 bits (382), Expect = 4.660e-39 Identity = 104/287 (36.24%), Postives = 138/287 (48.08%), Query Frame = 2
Query: 449 SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ-AEALEIKISPILVES----IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCS 1252
S C D SC +G ACCP + C D HCC + C+ C IPA + ++ CPDG S C T TCC++SD S+GCCP AVCC D + CCP N+ C++ G C S + K L + + RV C D + C DG TCC+ +YGCC +A CCSD +HCCP T C++ C Q E + P + E + C + C K TCC +++ + CCPY AVCC DH HCCP+ +CS
Sbjct: 63 SGCPDEYSCVNTPEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYC--------------IPASNQSAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLG-DEERRVPCLDGTF-CPDGSTCCEQ-VDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIREESANQVPCDATTSCPDKNTCCHLSSEKWGCCPYAQAVCCDDHIHCCPSGFTCS 332 HSP 8 Score: 112.464 bits (280), Expect = 7.753e-26 Identity = 90/323 (27.86%), Postives = 135/323 (41.80%), Query Frame = 2
Query: 401 LKVPASSILSVVCPDQSQCDDGSSCCVM-ADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----DH-----------QAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFPIIN 1306
L + S++ + +CPD S C + S CC + + CCP + EVV++ I + ++ S CPD S T +G CCP + C D CC + C+ C + + + V+CPD + +C TCC + S+GCCP A CC D +HCCP N+ C++ +G C DH ++E L++ + C +G+ C TCC + +Y CC +AVCCSDH HCCP T+C L K C + +L + P I
Sbjct: 7 LLLVVSTVSATLCPDGSTCGEKSLCCELPGKKGYGCCPAA------------------------------EVVSRSLPMILSQTSCS-GCPDEYSCVNT-------PEGGTACCPLAEGKSCQDGHHCCSVGSYCSDDGHYCIPASNQ-------------SAVVCPDGRSECPTLTTCCMMSDMSSWGCCPMPQAVCCDDHMHCCPHNSECDVQQGRCISNQDHIPWMSKLPARVKSEGLKLVGLGDEERRVPCLDGTFCPDGSTCCEQVDHTYGCCSILSAVCCSDHLHCCPGGTTCDLVHKKCVSQTGEGPLLPQMPAIR 278
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 229.18 bits (583), Expect = 3.037e-68 Identity = 141/371 (38.01%), Postives = 197/371 (53.10%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-----VLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPN--PVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALE----IKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-LLKFPII 1303
+ DGS+ CCP A CC D+ HCCP C+L C S L + K S SVVCPD ++QC D S+CC + G + CCP +A+CCSD HCCPQ+TVC+L C SK T + K+P ++C D +C G TCC L+ G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P P +++ D +C TCCKL +GD +GCCP A CCSD HCCPQ C + +G C D LE + +P+ + I C + C TCCPS S++CC P+AVCC D HCCP +C++ ++C+K++ ++ P++
Sbjct: 153 IMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSK--NYTTDLLTKLPGYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGD-WGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVL 518 HSP 2 Score: 223.016 bits (567), Expect = 5.956e-66 Identity = 141/363 (38.84%), Postives = 186/363 (51.24%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDS---------KLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---QAEALEIKISP-------ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+T G+ SCCP+S V C D HCCPQ C+ + +C ++ + + +V CP Q +C D ++CC+M DG++ CCP A CC D+ HCCP C+L C S K T + +P S+ CPD + C +TCC L G YGCCP NA+CCSD L CCPQ+TVC+L C SK LL K PV V C D + C +G TCC+L TG ++GCCP++ A CC D IHCCP C+ KGTC+ Q ++ I+P IL C + + C TCC + + CCP P AVCCSD+ HCCP +C L C K
Sbjct: 88 LTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLP----FSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTK-LPGYPVKEVKC-DMEVSCPEGYTCCRLNTG-AWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKG 430 HSP 3 Score: 210.305 bits (534), Expect = 3.415e-61 Identity = 130/364 (35.71%), Postives = 177/364 (48.63%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT----FQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G Y CCP +A+CCSD HCCPQ+T C+L + C S K T + K+P + V C + C +G +CC + G + CCP++ AVCC D HCCP C+ GTC+ +K+V+ ++L P I C D + C T TCC L+ G +GCCP AVCCSD CCPQ C L G C + L K P + + C D C GQTCC G S+ CC A CC D+ HCCP CN+ TC+ + ++ + L V +++C G C TCC + ++CCPY VCC D HCCP CS C + + ++ + P
Sbjct: 239 GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLS--KNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPC-DDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGC-DQHTSCPVGQTCCPSLKG-SWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDP 596 HSP 4 Score: 189.119 bits (479), Expect = 2.387e-53 Identity = 115/299 (38.46%), Postives = 149/299 (49.83%), Query Frame = 2
Query: 416 SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
S L C C G SC + G +CCP+S V C D HCCPQ C+ +C ++ +++CP C ATCC++ DGS+GCCP A CC D++ CCP C+L C S T +LL K F NR V+CPDAK QC D TCC+LPTG YGCCP A CCSD +HCCPQ+TVC++ + C + L K+ V+ +KC C + TCC ++ CCP+ AVCC DH HCCP C +C+ IL
Sbjct: 67 SHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLL-KKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTG-KYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGIL 351
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 105.531 bits (262), Expect = 1.565e-25 Identity = 76/258 (29.46%), Postives = 109/258 (42.25%), Query Frame = 2
Query: 569 CNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
C+ GTC+ +K+V+ ++L P I C D + C T TCC L+ G +GCCP C + AG K P+ Q + + C D C GQTCC G S+ CC A CC D+ HCCP CN+ TC+ + ++ + L V +++C G C TCC + ++CCPY VCC D HCCP CS C + + ++ + P
Sbjct: 4 CHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPC-DDFTRCPTNNTCCKLNSGDWGCCPIPEGYCQKGD----------TMVAGL----EKIPARQTTPLQ---IGDIGC-DQHTSCPVGQTCCPSLKG-SWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDP 241 HSP 2 Score: 100.138 bits (248), Expect = 1.315e-23 Identity = 76/251 (30.28%), Postives = 110/251 (43.82%), Query Frame = 2
Query: 338 ECNLNKGTCD------SVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP----DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTK--KPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC 1054
+C+ KGTC+ +K+V+ ++L P C D ++C ++CC + G++ CCP C K +V ++ KIPA ++ D ++C G TCC GS+ CC +AVCC D+ CCP CN+ A TC+ +P +L+ T P V V C + + C D QTCCK G + CCPY CC D HCCP C+ C
Sbjct: 3 QCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEGYC----------------------QKGDTMVAGLE-KIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLL-TLGPK-VGNVECGEGHF-CHDNQTCCKDSAG-VWACCPYLKGVCCRDGRHCCPGGFHCSARGTKC 226 HSP 3 Score: 88.9669 bits (219), Expect = 1.216e-19 Identity = 62/208 (29.81%), Postives = 90/208 (43.27%), Query Frame = 2
Query: 302 CSDKQHCCPQNTECNLNKGTC-------------DSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKI-PAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKTKKPSLLIKTFQPNPV 880
C D C NT C LN G D+++ + + I + C + C G +CC G++ACC AVCC D+QHCCP CN+ A TC+ + + + L + P + N +EC +G C TCC S G + CCP+ VCC D CCP C+ C K + + ++ P P+
Sbjct: 42 CDDFTRCPTNNTCCKLNSGDWGCCPIPEGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGN--VECGEG-HFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDPVPRPL 246 HSP 4 Score: 82.0333 bits (201), Expect = 3.055e-17 Identity = 47/131 (35.88%), Postives = 65/131 (49.62%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNL 655
GS++CC AVCC D+QHCCP CN+ TC+ + + + L + + +V C + C D +CC + G +ACCPY VCC D +HCCP C+ C K K M L+ P L
Sbjct: 118 GSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTL-GPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRK-KIPRWDMFLRDPVPRPL 246
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 104.375 bits (259), Expect = 1.907e-24 Identity = 71/182 (39.01%), Postives = 97/182 (53.30%), Query Frame = 2
Query: 449 SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTK--KPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPY 976
++C ++CC + G++ CCP AVCCSD QHCCPQ C L+ G C K +V ++ KIPA ++ D ++C G TCC GS+ CC +AVCC D+ CCP CN+ A TC+ +P +L+ T P V V C + + C D QTCCK G + CCPY
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQ-KGDTMVAGLE-KIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLL-TLGPK-VGNVECGEGHF-CHDNQTCCKDSAG-VWACCPY 177 HSP 2 Score: 100.908 bits (250), Expect = 2.960e-23 Identity = 63/167 (37.72%), Postives = 85/167 (50.90%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASS-----ILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKI-PAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPF 745
G + CCP AVCCSD QHCCPQ C L +G C + + K+PA I + C + C G +CC G++ACC AVCC D+QHCCP CN+ A TC+ + + + L + P + N +EC +G C TCC S G + CCP+
Sbjct: 17 GDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQK--GDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGN--VECGEG-HFCHDNQTCCKDSAGVWACCPY 177 HSP 3 Score: 95.5153 bits (236), Expect = 2.826e-21 Identity = 65/178 (36.52%), Postives = 85/178 (47.75%), Query Frame = 2
Query: 680 STCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNP--VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPY 1192
+ C T TCC L+ G +GCCP AVCCSD CCPQ C L G C + L I Q P + + C D C GQTCC G S+ CC A CC D+ HCCP CN+ TC+ + ++ + L V +++C G C TCC + ++CCPY
Sbjct: 3 TRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGC-DQHTSCPVGQTCCPSLKG-SWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPY 177 HSP 4 Score: 94.3597 bits (233), Expect = 5.577e-21 Identity = 54/139 (38.85%), Postives = 79/139 (56.83%), Query Frame = 2
Query: 911 QCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALE----IKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-LLKFPII 1303
+C TCCKL +GD +GCCP A CCSD HCCPQ C + +G C D LE + +P+ + I C + C TCCPS S++CC P+AVCC D HCCP +C++ ++C+K++ ++ P++
Sbjct: 4 RCPTNNTCCKLNSGD-WGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVL 140
BLAST of Granulin precursor vs. Ensembl Mouse
Match: Grn (granulin [Source:MGI Symbol;Acc:MGI:95832]) HSP 1 Score: 98.2117 bits (243), Expect = 3.511e-23 Identity = 62/174 (35.63%), Postives = 80/174 (45.98%), Query Frame = 2
Query: 416 SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQC 916
S L C C G SC + G +CCP+S V C D HCCPQ C+ +C ++ +++CP C ATCC++ DGS+GCCP A CC D++ CCP C+L C S T +LL K F NR V+CPDAK QC
Sbjct: 54 SHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLL-KKFPAQKTNRAVSLPFSVVCPDAKTQC 214 HSP 2 Score: 83.9593 bits (206), Expect = 3.620e-18 Identity = 51/156 (32.69%), Postives = 74/156 (47.44%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDS---------KLKEVVTKMKLKIPAISNLSIECPDGSSTC 688
+T G+ SCCP+S V C D HCCPQ C+ + +C ++ + + +V CP Q +C D ++CC+M DG++ CCP A CC D+ HCCP C+L C S K T + +P S+ CPD + C
Sbjct: 75 LTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLP----FSVVCPDAKTQC 214 HSP 3 Score: 82.4185 bits (202), Expect = 1.052e-17 Identity = 45/123 (36.59%), Postives = 62/123 (50.41%), Query Frame = 2
Query: 686 CKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC 1054
C G +C L G+ CCPFS V C D CCPQ C+ +C + NP+ V CP ++++C D TCC + G S+GCCP A+CC D++HCCP C++ C
Sbjct: 67 CPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSCFQMSD-----------NPLGAVQCPGSQFECPDSATCCIMVDG-SWGCCPMPQASCCEDRVHCCPHGASCDLVHTRC 177 HSP 4 Score: 71.633 bits (174), Expect = 7.239e-14 Identity = 43/127 (33.86%), Postives = 58/127 (45.67%), Query Frame = 2
Query: 935 CKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFP 1297
C L + CCP+S C D HCCPQ C+ + +C ++S + +++CP C TCC + S+ CCP P A CC D HCCP+ SC L C +L KFP
Sbjct: 73 CLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC--------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFP 191
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28799|GRN_HUMAN (Progranulin OS=Homo sapiens OX=9606 GN=GRN PE=1 SV=2) HSP 1 Score: 234.572 bits (597), Expect = 1.644e-69 Identity = 140/350 (40.00%), Postives = 187/350 (53.43%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPI-------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
T G+ SCCP+ AV C D HCCP+ C+ + +C + +S+ ++ CPD Q +C D S+CCVM DG++ CCP A CC D+ HCCP C+L C + K++ + + A+S+ S+ CPD S C G+TCC L G YGCCP NA CCSD L CCPQ+TVC+L C SK + L+ + V V C D + C DG TCC+L +G ++GCCP++ A CC D IHCCP C+ KGTC+ HQ +E + + L + C N S C TCC + + CCP P AVCCSDH HCCP +C
Sbjct: 75 FTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQ-----------RSGNNSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGTHPLAKKLPAQRTNRAVALSS-SVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSG-AWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC 410 HSP 2 Score: 227.639 bits (579), Expect = 5.312e-67 Identity = 140/370 (37.84%), Postives = 193/370 (52.16%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSI-------LSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF------QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE-IKISPILVESIKCPNGSLCGQK------GTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFP 1297
V DGS+ CCP A CC D+ HCCP C+L C + + K+PA SV+CPD +S+C DGS+CC + G + CCP +A CCSD HCCPQ+TVC+L C SK + T + K+PA + ++C D +C G TCC L G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P + R + D C TCC+L +G+ +GCCP A CCSD HCCPQ C + +G C +E + ++ P S+ P C Q TCCPS S++CC P+AVCC D HCCP +C++ +SC+K ++ P
Sbjct: 141 VMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRC--ITPTGTHPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSGKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKC-DMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGE-WGCCPIPEAVCCSDHQHCCPQGYTC-VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCPSLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQP 504 HSP 3 Score: 210.69 bits (535), Expect = 1.378e-60 Identity = 126/361 (34.90%), Postives = 177/361 (49.03%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV-------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE----IKISP-ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G Y CCP +A CCSD HCCPQ+T C+L + C S + T + K+PA ++ V C + C DG +CC + G + CCP++ AVCC D HCCP C+ GTC+ +V K L +P L + P D S+C + TCC L+ G +GCCP AVCCSD CCPQ C + + ++ S ++ + P R I D C GQTCC G S+ CC A CC D+ HCCP CN+ +C+ + + + + SP + V+ ++C G C TCC ++CCPY VCC+D HCCP C+ C + ++
Sbjct: 227 GKYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSK-ENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQGPHQVPWMEKAPAHLSLPDPQALKRDVPCDNVSSCPSSDTCCQLTSGEWGCCPIPEAVCCSDHQHCCPQGYTC-----VAEGQCQRGSEIVAGLEKMPARRASLSHPRDIGCDQHTSCPVGQTCCP-SLGGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARSCEKEVVSAQPATFLARSPHVGVKDVECGEGHFCHDNQTCCRDNRQGWACCPYRQGVCCADRRHCCPAGFRCAARGTKCLRREAPRW 580 HSP 4 Score: 183.341 bits (464), Expect = 2.018e-50 Identity = 112/304 (36.84%), Postives = 150/304 (49.34%), Query Frame = 2
Query: 398 KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
K S L C + C G SC G +CCP+ AV C D HCCP+ C+ +C + S +I+CPD C +TCC++ DGS+GCCP A CC D++ CCP C+L C + T L K NR V+CPDA+ +C DG TCC+LP+G YGCCP ATCCSD +HCCPQ+TVC++ + C + A L K+ V +KC C TCC + ++ CCP+ AVCC DH HCCP +C +C++
Sbjct: 48 KWPTTLSRHLGGPCQVDAHCSAGHSCIFTVSGTSSCCPFPEAVACGDGHHCCPRGFHCSADGRSCFQRSGN-----------NSVGAIQCPDSQFECPDFSTCCVMVDGSWGCCPMPQASCCEDRVHCCPHGAFCDLVHTRCITPTGT-HPLAKKLPAQRTNRAVALSSSVMCPDARSRCPDGSTCCELPSG-KYGCCPMPNATCCSDHLHCCPQDTVCDLIQSKCLSKENATTDLLTKLPAHTVGDVKCDMEVSCPDGYTCCRLQSGAWGCCPFTQAVCCEDHIHCCPAGFTCDTQKGTCEQG 338
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28798|GRN_MOUSE (Progranulin OS=Mus musculus OX=10090 GN=Grn PE=1 SV=2) HSP 1 Score: 229.18 bits (583), Expect = 1.498e-67 Identity = 141/371 (38.01%), Postives = 197/371 (53.10%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-----VLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPN--PVNRVICPDAK----YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALE----IKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-LLKFPII 1303
+ DGS+ CCP A CC D+ HCCP C+L C S L + K S SVVCPD ++QC D S+CC + G + CCP +A+CCSD HCCPQ+TVC+L C SK T + K+P ++C D +C G TCC L+ G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P P +++ D +C TCCKL +GD +GCCP A CCSD HCCPQ C + +G C D LE + +P+ + I C + C TCCPS S++CC P+AVCC D HCCP +C++ ++C+K++ ++ P++
Sbjct: 140 IMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSK--NYTTDLLTKLPGYPVKEVKC-DMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGD-WGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVL 505 HSP 2 Score: 223.016 bits (567), Expect = 2.751e-65 Identity = 141/363 (38.84%), Postives = 186/363 (51.24%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDS---------KLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---QAEALEIKISP-------ILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+T G+ SCCP+S V C D HCCPQ C+ + +C ++ + + +V CP Q +C D ++CC+M DG++ CCP A CC D+ HCCP C+L C S K T + +P S+ CPD + C +TCC L G YGCCP NA+CCSD L CCPQ+TVC+L C SK LL K PV V C D + C +G TCC+L TG ++GCCP++ A CC D IHCCP C+ KGTC+ Q ++ I+P IL C + + C TCC + + CCP P AVCCSD+ HCCP +C L C K
Sbjct: 75 LTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLLKKFPAQKTNRAVSLP----FSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTK-LPGYPVKEVKC-DMEVSCPEGYTCCRLNTG-AWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPCDDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKG 417 HSP 3 Score: 210.69 bits (535), Expect = 1.542e-60 Identity = 130/364 (35.71%), Postives = 177/364 (48.63%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT----FQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G Y CCP +A+CCSD HCCPQ+T C+L + C S K T + K+P + V C + C +G +CC + G + CCP++ AVCC D HCCP C+ GTC+ +K+V+ ++L P I C D + C T TCC L+ G +GCCP AVCCSD CCPQ C L G C + L K P + + C D C GQTCC G S+ CC A CC D+ HCCP CN+ TC+ + ++ + L V +++C G C TCC + ++CCPY VCC D HCCP CS C + + ++ + P
Sbjct: 226 GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLS--KNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGILQVPWMKKVIAPLRLPDPQILKSDTPC-DDFTRCPTNNTCCKLNSGDWGCCPIPEAVCCSDNQHCCPQGFTC-LAQGYCQKGDTMVAGLEKIPARQTTPLQIGDIGC-DQHTSCPVGQTCCPSLKG-SWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDVDFIQPPVLLTLGPKVGNVECGEGHFCHDNQTCCKDSAGVWACCPYLKGVCCRDGRHCCPGGFHCSARGTKCLRKKIPRWDMFLRDP 583 HSP 4 Score: 189.119 bits (479), Expect = 1.277e-52 Identity = 115/299 (38.46%), Postives = 149/299 (49.83%), Query Frame = 2
Query: 416 SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR-------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
S L C C G SC + G +CCP+S V C D HCCPQ C+ +C ++ +++CP C ATCC++ DGS+GCCP A CC D++ CCP C+L C S T +LL K F NR V+CPDAK QC D TCC+LPTG YGCCP A CCSD +HCCPQ+TVC++ + C + L K+ V+ +KC C + TCC ++ CCP+ AVCC DH HCCP C +C+ IL
Sbjct: 54 SHHLDGSCQTHGHCPAGYSCLLTVSGTSSCCPFSKGVSCGDGYHCCPQGFHCSADGKSC------------FQMSDNPLGAVQCPGSQFECPDSATCCIMVDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCVSPTGTHTLL-KKFPAQKTNRAVSLPFSVVCPDAKTQCPDDSTCCELPTG-KYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCLSKNYTTDLLTKLPGYPVKEVKCDMEVSCPEGYTCCRLNTGAWGCCPFAKAVCCEDHIHCCPAGFQCHTEKGTCEMGIL 338
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P23785|GRN_RAT (Progranulin OS=Rattus norvegicus OX=10116 GN=Grn PE=1 SV=3) HSP 1 Score: 228.409 bits (581), Expect = 2.426e-67 Identity = 141/371 (38.01%), Postives = 192/371 (51.75%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD----------HQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-----LLKFPI 1300
+T G+ SCCP+S V C D QHCCP+ C+ + +C + ++ ++ P S Q +C D ++CC+M DG++ CCP A CC D+ HCCP C+L C S LK+ + + A+++ S+ CPD + C +TCC L G YGCCP NA+CCSD L CCPQ+TVC+L C SK L+ K PVN V C D + C DG TCC+L TG ++GCCP++ A CC D IHCCP C+ GTC+ +L + IL + C + S C TCC ++ + CCP P AVCC DH HCCP C ++ C K L K P+
Sbjct: 75 LTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISDSLLGAVQ--CPGS---------QFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLLKKFPAQRTNR--AVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTK-LPGYPVNEVKC-DLEVSCPDGYTCCRLNTG-AWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSSCPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPV 428 HSP 2 Score: 221.476 bits (563), Expect = 1.266e-64 Identity = 143/374 (38.24%), Postives = 199/374 (53.21%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS------VLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQ----PNPV---NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALE---IKISPILVES-IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
+ DGS+ CCP A CC D+ HCCP C+L C S +LK+ + + AS SVVCPD ++QC D S+CC + G + CCP +A+CCSD HCCPQ+TVC+L C SK + T + K+P ++C D +C G TCC L+ G++GCCPF+ AVCC D + CCP C+ GTC+ + + K P+P N V C D C TCC+L +GD +GCCP A CC D HCCPQ C +++G C D LE ++ + +L I C + C TCCPS S++CC P+AVCC D HCCP +C++ ++C+K+ P ++
Sbjct: 140 IMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLLKKFPAQRTNRAVAS--FSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISK--DYTTDLMTKLPGYPVNEVKC-DLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFS-SCPSNNTCCRLSSGD-WGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQPSMDL 505 HSP 3 Score: 209.92 bits (533), Expect = 2.707e-60 Identity = 130/367 (35.42%), Postives = 179/367 (48.77%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCD------SKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV-------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G Y CCP +A+CCSD HCCPQ+T C+L + C + K+ T + K+P + V C + C DG +CC + G + CCP++ AVCC D HCCP C+ GTC+ +K+V + L P I + C D SS C + TCC LS G +GCCP AVCC D CCPQ C + G C +K ++ + PV + I D C GQTCC G S+ CC A CC D+ HCCP CN+ TC+ A +++ + V +++C G C +CC + ++CCPY VCC D HCCP CS C + ++ I+ P
Sbjct: 225 GKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKC--ISKDYTTDLMTKLPGYPVNEVKCDLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVLQVPWMKKVTASLSLPDPQILKNDVPCDDFSS-CPSNNTCCRLSSGDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYC----QKGDRMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLKG-SWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQPSMDLTFGSKVGNVECGAGHFCHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKTPRWDILLRDP 582 HSP 4 Score: 191.43 bits (485), Expect = 1.716e-53 Identity = 117/302 (38.74%), Postives = 153/302 (50.66%), Query Frame = 2
Query: 407 VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNR------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
+ S L C + C DG SC + G +CCP+S V C D QHCCP+ C+ +C ++ +++CP C ATCC++ DGS+GCCP A CC D++ CCP C+L C S T LL K F NR V+CPDAK QC D TCC+LPTG YGCCP A CCSD +HCCPQ+TVC++ + C D+ + L K+ V +KC C TCC ++ CCP+ AVCC DH HCCP C T +C+ +L
Sbjct: 51 IITSRRLDGSCQIRDHCPDGYSCLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISDSLLG------------AVQCPGSQFECPDSATCCIMIDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLL-KKFPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPTG-KYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTD-LMTKLPGYPVNEVKCDLEVSCPDGYTCCRLNTGAWGCCPFTKAVCCEDHIHCCPAGFQCHTETGTCELGVL 337 HSP 5 Score: 123.635 bits (309), Expect = 4.175e-29 Identity = 74/212 (34.91%), Postives = 105/212 (49.53%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS---VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKTKKPSLLIKTFQPNPV 880
+ G + CCP AVCC D QHCCPQ +C +++G C + V K+ V +++L + C + C G +CC G++ACC AVCC D+QHCCP CN+ A TC+ V M L + ++EC G C +CC S G + CCP+ VCC D CCP C+ C KT + +L++ P P+
Sbjct: 379 SSGDWGCCPMPEAVCCLDHQHCCPQGFKC-MDEGYCQKGDRMVAGLEKMPVRQTTLLQHGDIGCDQHTSCPVGQTCCPSLKGSWACCQLPHAVCCEDRQHCCPAGYTCNVKARTCEKDAGSVQPSMDLTFGSKVG-NVECGAG-HFCHDNQSCCKDSQGGWACCPYVKGVCCRDGRHCCPIGFHCSAKGTKCLRKKTPRWDILLRDPAPRPL 587 HSP 6 Score: 111.694 bits (278), Expect = 4.266e-25 Identity = 81/243 (33.33%), Postives = 111/243 (45.68%), Query Frame = 2
Query: 611 VVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-DHQAEALEIKISP-------ILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFP 1297
++T +L + CPDG S C L G+ CCPFS V C D CCP+ C+ +C + SLL V CP ++++C D TCC + G S+GCCP A+CC D++HCCP C++ C +K P + S+ CP+ + C TCC Y CCP PNA+CCSDH HCCP +T C L C +++ K P
Sbjct: 51 IITSRRLD--GSCQIRDHCPDGYS-------CLLTVSGTSSCCPFSEGVSCDDGQHCCPRGFHCSADGKSCSQISD--SLL---------GAVQCPGSQFECPDSATCCIMIDG-SWGCCPMPQASCCEDRVHCCPHGASCDLVHTRCISPTGTHPLLKKFPAQRTNRAVASFSVVCPDAKTQCPDDSTCCELPTGKYGCCPMPNAICCSDHLHCCPQDTVCDLIQSKCISKDYTTDLMTKLP 272
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|P28797|GRN_CAVPO (Progranulin OS=Cavia porcellus OX=10141 GN=GRN PE=1 SV=2) HSP 1 Score: 189.504 bits (480), Expect = 1.138e-52 Identity = 115/291 (39.52%), Postives = 154/291 (52.92%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-CPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKE--VVTKMKLK-----------------IPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH 1060
+T G+ +CCP+S A+ C D HCCP C+ + GTC ++ P +L V CP + +C D S+CC M DG++ CCP A CC D+ HCCP C+L C + L + TK+ + +PA S+ CPD S C TCCLL+ G YGCCP NA+CCSD L CCPQ+TVC+L C S+ K +LL K V V C D + C +GQTCC+L +G +GCCP+ A CC D +HCCP+ C+ K TC+
Sbjct: 75 LTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTC------------IQRPDIHLLGAVQCPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTK-LPSWTVWDVEC-DQEVSCPEGQTCCRLQSG-KWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQ 350 HSP 2 Score: 189.504 bits (480), Expect = 1.355e-52 Identity = 126/378 (33.33%), Postives = 178/378 (47.09%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLK-------------------VPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT-------------------FQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK 1276
DGS+ CCP A CC D+ HCCP C+L C + L KL +PA+ SV+CPD +SQC D ++CC++A G + CCP +A+CCSD HCCPQ+TVC+L C S+ K + K+P+ + +EC D +C G TCC L G +GCCPF AVCC D + CCP+ C+ TC+ + KT P P+ I D C+ G CC+L +G+ +GCCP S + + C C + + A + +S + C + C TCCP ++CC P+AVCC D HCCP +C++ +SC+K
Sbjct: 144 DGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNK--AKTLLTKLPSWTVWDVEC-DQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISCDEVVSCAPGNICCRLASGE-WGCCP-------SSEGYLCMAGERCQVGDRLAPEKMAAHLMSLS--QTTDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEK 508 HSP 3 Score: 189.504 bits (480), Expect = 1.547e-52 Identity = 119/331 (35.95%), Postives = 162/331 (48.94%), Query Frame = 2
Query: 395 IKLKVPA-SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNL-SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKT---------FQPNPV-----------NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
+ L+ A S L C + C G SC + A G ACCP+S A+ C D HCCP C+ GTC ++ P I L +++CP G C +TCC + DGS+GCCP A CC D++ CCP C+L C + L K + PV VICPD++ QC D TCC L +G+ YGCCP A CCSD +HCCPQ+TVC++ + C ++A+ L K+ V ++C C + TCC + + CCP+P AVCC DH HCCP C +C++ LL+ P P
Sbjct: 46 VDLRTTALSGYLGRPCQSPANCPIGHSCVLTAAGTAACCPFSQAMACGDGHHCCPYGFHCSTDGGTC------------IQRPDIHLLGAVQCPGGEFECPDSSTCCHMLDGSWGCCPMPQASCCEDRVHCCPHGASCDLVHIRCVTALGSHPLTTKLPAQRTNYTGAEGTPVVSPGLLPAALPTSVICPDSRSQCPDDTTCCLLASGE-YGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNKAKTLLTKLPSWTVWDVECDQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQG-LLQVPWAQKTP 362 HSP 4 Score: 169.474 bits (428), Expect = 3.170e-45 Identity = 116/374 (31.02%), Postives = 160/374 (42.78%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAIS-------------------NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVES------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC-SLNTKSCDKNILLKF 1294
+ G Y CCP +A+CCSD HCCPQ+T C+L + C S K + K+P+ ++ V C + C +G +CC + G + CCP+ AVCC D HCCP+ C+ TC+ L +V K I C D +C G CC L+ G +GCCP S C C G + K + L+ Q V D C GQTCC G + CC A CC D HCCP CN+ +C+ A+ + +P+ V S + C + C + TCC + ++CCP+ VCC D HCCP C S T+ K LL +
Sbjct: 237 LLASGEYGCCPMPNAICCSDHLHCCPQDTVCDLRQSRCLSQNK--AKTLLTKLPSWTVWDVECDQEVSCPEGQTCCRLQSGKWGCCPFPKAVCCEDHVHCCPEGFRCHTEKDTCEQGLLQVPWAQKTPAQPSRPSQPSPPGPPGPPSPPGPLRSEISC-DEVVSCAPGNICCRLASGEWGCCPSSEGYLCMAGERC---------QVGDRLAPEKMAAHLMSLSQTTDVGC----DQHASCPVGQTCCP-KLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAADGAHLA-APLAVGSTGGVMDVACGDRHFCHDEQTCCRDSRGGWACCPFHQGVCCKDQRHCCPAGFHCESQGTRCVHKKSLLHW 592 HSP 5 Score: 87.4261 bits (215), Expect = 3.619e-17 Identity = 62/207 (29.95%), Postives = 80/207 (38.65%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISN-LSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV 880
G + CCP S C + C D + E + + + S V C + C G +CC G +ACC AVCC D QHCCP CN+ A +C+ L + + + + C D C TCC S G + CCPF VCC D+ CCP C C K SLL P P
Sbjct: 410 GEWGCCPSSEGYLCMAGERC-----------QVGDRLAPEKMAAHLMSL--SQTTDVGCDQHASCPVGQTCCPKLGGGWACCQLPHAVCCEDGQHCCPAGYTCNVKARSCEKAADGAHLAAPLAVGSTGGVMDVACGD-RHFCHDEQTCCRDSRGGWACCPFHQGVCCKDQRHCCPAGFHCESQGTRC---VHKKSLLHWDSLPRPA 599
BLAST of Granulin precursor vs. UniProt/SwissProt
Match: sp|Q54QR7|GRN_DICDI (Granulin OS=Dictyostelium discoideum OX=44689 GN=grn PE=3 SV=1) HSP 1 Score: 63.5438 bits (153), Expect = 5.189e-11 Identity = 31/65 (47.69%), Postives = 39/65 (60.00%), Query Frame = 2
Query: 1106 SIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPI 1300
S+KCP+GSLC TCC +++ SY+CCP PNA CCSD HCCP +C C L+F
Sbjct: 64 SVKCPDGSLCPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGGNICKPQQGLRFSF 128 HSP 2 Score: 57.7658 bits (138), Expect = 6.089e-9 Identity = 28/55 (50.91%), Postives = 35/55 (63.64%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC 592
SV CPD S C + ++CC +DG++ACCP +A CCSDKQHCCP C C
Sbjct: 64 SVKCPDGSLCPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGGNIC 118 HSP 3 Score: 55.0694 bits (131), Expect = 5.016e-8 Identity = 33/70 (47.14%), Postives = 38/70 (54.29%), Query Frame = 2
Query: 617 TKMKLKIPAISNL-SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC 823
TK L++ + S++CPDGS C TCC SDGSY CCP NA CCSDK CCP C C
Sbjct: 50 TKKSLELTETQDFGSVKCPDGS-LCPNSNTCCSASDGSYACCPTPNAQCCSDKQHCCPYQFTCGNGGNIC 118
BLAST of Granulin precursor vs. TrEMBL
Match: V4ARK5 (Uncharacterized protein (Fragment) OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_146967 PE=4 SV=1) HSP 1 Score: 268.855 bits (686), Expect = 1.460e-82 Identity = 157/352 (44.60%), Postives = 202/352 (57.39%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV-LKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIE---CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ-----AEALEIKISPILVESIKCPN-GSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
G Y CCP +AVCCSD+ HCCP T C+ G C+ V+ K + + +VVCPDQ SQC DGS+CC ++ G + CCP +AVCCSD+ HCCP T C+ AG C+ ++ + M K + +L +E CPD S C G+TCC LS G YGCCP NAVCCSD++ CCP T C+ AG C+ + + K + Q V V+CPD + QC DG TCCKL TG YGCCP A CCSD+IHCCP T C+ G C+ Q + + + VE++ CP+ S C TCC + Y CCP PNAVCCSD HCCP+ T+C CD+
Sbjct: 13 GQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCN--RQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQ-YGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCDRQ 361 HSP 2 Score: 226.868 bits (577), Expect = 2.006e-66 Identity = 131/291 (45.02%), Postives = 168/291 (57.73%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV-LKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIE---CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIK 1114
G Y CCP +AVCCSD+ HCCP T C+ G C+ V+ K + + +VVCPDQ SQC DGS+CC ++ G + CCP +AVCCSD+ HCCP T C+ AG C+ ++ + M K + +L +E CPD S C G+TCC LS G YGCCP NAVCCSD++ CCP T C+ AG C+ + + K + Q V V+CPD + QC DG TCCKL TG YGCCP A CCSD+IHCCP T C+ G CD Q + +S+K
Sbjct: 91 GQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCN--RQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQ-YGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCDRQDGTSVMWSKKTSSQSLK 378 HSP 3 Score: 222.631 bits (566), Expect = 1.154e-64 Identity = 127/286 (44.41%), Postives = 165/286 (57.69%), Query Frame = 2
Query: 455 CDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIE---CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ-----AEALEIKISPILVESIKCPN-GSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
C DGS+CC ++ G + CCP +AVCCSD+ HCCP T C+ AG C+ ++ + M K + +L +E CPD S C G+TCC LS G YGCCP NAVCCSD++ CCP T C+ AG C+ + + K + Q V V+CPD + QC DG TCCKL TG YGCCP A CCSD+IHCCP T C+ G C+ Q + + + VE++ CP+ S C TCC + Y CCP PNAVCCSD HCCP+ T+C C++
Sbjct: 1 CPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCN--RQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQ-YGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQDGTSVMWSKKTSSQSLKVENVVCPDQQSQCPDGSTCCKLSTGQYGCCPLPNAVCCSDQIHCCPSGTTCDTGAGKCNRQ 283
BLAST of Granulin precursor vs. TrEMBL
Match: K1PAM6 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10005248 PE=4 SV=1) HSP 1 Score: 282.337 bits (721), Expect = 2.791e-82 Identity = 161/386 (41.71%), Postives = 223/386 (57.77%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC---DSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV----------NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----------------DHQAEALEIKISPILV----ESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G + CCP AVCCSD +HCCP++T+C +++G C DS+ + K+ PA +VVCPD QSQC DG +CC +A G + CCP AVCCSD +HCCP+NT C++S+G C + E+ K+PA+ + ++ CPDG S CKT TCC L+ G+YGCCP AVCCSD CCP+NT C++ +G C T+ L++ F+ P+ + V+CPD +C DGQTCC L +G YGCCP A CCSD +HCCPQ T C+I + C Q + E +L+ E+++CP+G S C TCC +A +Y CCP P AVCCSD HCCP T+C +++ C++ ++ P
Sbjct: 393 GQWGCCPIPKAVCCSDGKHCCPESTKCEVSQGKCIRADSLSMDWFEKL----PALRTRNVVCPDGQSQCKDGQTCCKLATGQYGCCPIPKAVCCSDGKHCCPENTQCDVSSGKC-KRGDELSMDWFAKLPALRSKNVVCPDGQSECKTSQTCCKLASGNYGCCPIPKAVCCSDMKHCCPENTKCDVSSGKC---TRGDGLIVDWFEKMPLAVRSRGQGEASSVMCPDGASECEDGQTCCLLASG-KYGCCPIQDAVCCSDHVHCCPQGTKCDIQEKKCIGKPAANLGVISMDMIQIQGKGQEKSHPELLLKLPTENVECPDGQSECKTGQTCCKTAAGNYGCCPLPKAVCCSDMKHCCPEGTTCDVSSGKCNRGNTYVMDWMDKQP 769 HSP 2 Score: 279.641 bits (714), Expect = 2.555e-81 Identity = 150/361 (41.55%), Postives = 212/361 (58.73%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNP----VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI----KISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G+Y CCP AVCCSD +HCCP+ T C+++ G C+ V+ + + SV+CPD Q +C DG +CC +A G + CCP AVCCSD +HCCP+NT C +S G C E++ +K + + + ++ CPDG S CK+G TCC L+ G+YGCCP AVCCSD CCP+ T C++ +G C+ + + ++ P V+CPD +++C DGQTCCKL +G +GCCP A CCSD HCCP++T C +++G C +A++L + K+ + ++ CP+G S C TCC A Y CCP P AVCCSD HCCP NT C +++ C + L P
Sbjct: 162 GNYGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGSTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCSDGKHCCPENTKCEVSQGKCMRGTGEMLDWLKKEPAMLRSKNVVCPDGQSECKSGQTCCKLASGNYGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGS---TYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASG-QWGCCPIPKAVCCSDGKHCCPESTKCEVSQGKC-IRADSLSMDWFEKLPALRTRNVVCPDGQSQCKDGQTCCKLATGQYGCCPIPKAVCCSDGKHCCPENTQCDVSSGKCKRGDELSMDWFAKLP 517 HSP 3 Score: 271.166 bits (692), Expect = 3.219e-78 Identity = 145/361 (40.17%), Postives = 205/361 (56.79%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV----NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQA----EALEIKISPILVESIKCPNGS-LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G + CCP AVCC+D +HCCP+NT+C +++G C E++ + + +VVCPD QS+C G +CC +A GN+ CCP AVCCSD +HCCP+ T C++S+G C+ V+ M + + S+ CPDG CK G TCC L+ G +GCCP AVCCSD CCP+NT C + G C T + ++ + P V+CPD + +C GQTCCKL +G+ YGCCP A CCSD HCCP+ T C+++ G C+ + + ++ + + + S+ CP+G C TCC A+ + CCP P AVCCSD HCCP +T C ++ C + L P
Sbjct: 85 GQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCMRGTGEMLDWFEKEPALVRSENVVCPDGQSECKSGQTCCKLASGNYGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGSTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCSDGKHCCPENTKCEVSQGKCMRGTGE---MLDWLKKEPAMLRSKNVVCPDGQSECKSGQTCCKLASGN-YGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGSTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCSDGKHCCPESTKCEVSQGKCIRADSLSMDWFEKLP 441 HSP 4 Score: 270.011 bits (689), Expect = 8.878e-78 Identity = 156/384 (40.62%), Postives = 210/384 (54.69%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC---DSKLKEVVTKMKLKIPAISNL---SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK---------------------TKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD----HQAEALEIKISPILVESIKCPNGS-LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
G Y CCP AVCCSD +HCCP+NT+C+++ G C E+ K+PA +VVCPD QS+C +CC +A GN+ CCP AVCCSD +HCCP+NT C++S+G C D + + KM L + + S+ CPDG+S C+ G TCCLL+ G YGCCP +AVCCSD + CCPQ T C++ C K P LL+K P V CPD + +C GQTCCK G+ YGCCP A CCSD HCCP+ T C+++ G C+ + + ++ + + + S+ CP+G C TCC A+ + CCP P AVCC+D HCCP NT C ++ C + L P
Sbjct: 469 GQYGCCPIPKAVCCSDGKHCCPENTQCDVSSGKCKRG-DELSMDWFAKLPALRSKNVVCPDGQSECKTSQTCCKLASGNYGCCPIPKAVCCSDMKHCCPENTKCDVSSGKCTRGDGLIVDWFEKMPLAVRSRGQGEASSVMCPDGASECEDGQTCCLLASGKYGCCPIQDAVCCSDHVHCCPQGTKCDIQEKKCIGKPAANLGVISMDMIQIQGKGQEKSHPELLLKL----PTENVECPDGQSECKTGQTCCKTAAGN-YGCCPLPKAVCCSDMKHCCPEGTTCDVSSGKCNRGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCTRGTGLVLDWFEKLP 846 HSP 5 Score: 265.774 bits (678), Expect = 3.001e-76 Identity = 144/351 (41.03%), Postives = 207/351 (58.97%), Query Frame = 2
Query: 293 AVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNP----VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-DHQAEALE-IKISPILVES--IKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
AVCCSD +HCCP+ T C+++ G C V+ + + SV+CPD Q +C DG +CC +A G + CCP AVCC+D +HCCP+NT C +S G C E++ + + + + ++ CPDG S CK+G TCC L+ G+YGCCP AVCCSD CCP+ T C++ +G C+ + + ++ P V+CPD +++C DGQTCCKL +G +GCCP A CCSD HCCP+NT C +++G C E L+ +K P ++ S + CP+G S C TCC A+ +Y CCP P AVCCSD HCCP T+C +++ C++ ++ P
Sbjct: 18 AVCCSDMKHCCPEGTTCDVSSGKCKRGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCMRGTGEMLDWFEKEPALVRSENVVCPDGQSECKSGQTCCKLASGNYGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGS---TYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASG-QWGCCPIPKAVCCSDGKHCCPENTKCEVSQGKCMRGTGEMLDWLKKEPAMLRSKNVVCPDGQSECKSGQTCCKLASGNYGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGSTYVMDWMDKQP 364 HSP 6 Score: 247.669 bits (631), Expect = 1.432e-69 Identity = 148/370 (40.00%), Postives = 204/370 (55.14%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC---DSVLKEVVTKIKLKVPA---SSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK-----------LKEVVTKMKLK-----IPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV----NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQA-------EALEIKISPILVESIK------CPNGSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
G+Y CCP AVCCSD +HCCP+NT+C+++ G C D ++ + K+ L V + SV+CPD S+C+DG +CC++A G + CCP AVCCSD HCCPQ T C++ C K + ++ K + K + + ++ECPDG S CKTG TCC + G+YGCCP AVCCSD CCP+ T C++ +G C+ + + ++ P V+CPD +++C DGQTCCKL +G +GCCP A CC+D HCCP+NT C +++G C E L P LVE IK CP+ C K TCC + + CC Y NAVCC ++CCP C
Sbjct: 545 GNYGCCPIPKAVCCSDMKHCCPENTKCDVSSGKCTRGDGLIVDWFEKMPLAVRSRGQGEASSVMCPDGASECEDGQTCCLLASGKYGCCPIQDAVCCSDHVHCCPQGTKCDIQEKKCIGKPAANLGVISMDMIQIQGKGQEKSHPELLLKLPTENVECPDGQSECKTGQTCCKTAAGNYGCCPLPKAVCCSDMKHCCPEGTTCDVSSGKCN---RGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASG-QWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCTRGTGLVLDWFEKLPALKRPGLVEMIKKDNTIVCPDQKTSCPDKNTCCKNKEGKFGCCAYNNAVCCKSGTYCCPKGYIC 910 HSP 7 Score: 193.741 bits (491), Expect = 3.222e-50 Identity = 113/292 (38.70%), Postives = 153/292 (52.40%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNL-----------NKGTCDSVLKEVVTKIKLKVPASSIL-----SVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV-------------NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCN 1036
+ G Y CCP AVCCSD HCCPQ T+C++ N G + ++ K + K +L +V CPD QS+C G +CC A GN+ CCP AVCCSD +HCCP+ T C++S+G C+ V+ M + + S+ CPDG CK G TCC L+ G +GCCP AVCC+D CCP+NT C + G C T+ L++ F+ P N ++CPD K C D TCCK G +GCC Y+ A CC +CCP+ +C+
Sbjct: 624 LLASGKYGCCPIQDAVCCSDHVHCCPQGTKCDIQEKKCIGKPAANLGVISMDMIQIQGKGQEKSHPELLLKLPTENVECPDGQSECKTGQTCCKTAAGNYGCCPLPKAVCCSDMKHCCPEGTTCDVSSGKCNRGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKC---TRGTGLVLDWFEKLPALKRPGLVEMIKKDNTIVCPDQKTSCPDKNTCCKNKEG-KFGCCAYNNAVCCKSGTYCCPKGYICD 911 HSP 8 Score: 145.976 bits (367), Expect = 7.190e-34 Identity = 93/285 (32.63%), Postives = 131/285 (45.96%), Query Frame = 2
Query: 632 KIPAI---SNLSIECPDGSS---------------------------------------------TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV----NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQA----EALEIKISPILVESIKCPNGS-LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
K PA+ N+++ C D CK G TCC L+ G +GCCP AVCC+D CCP+NT C + G C T + ++ F+ P V+CPD + +C GQTCCKL +G+ YGCCP A CCSD HCCP+ T C+++ G C+ + + ++ + + + S+ CP+G C TCC A+ + CCP P AVCCSD HCCP NT C ++ C + + P
Sbjct: 7 KEPALVRSENVAVCCSDMKHCCPEGTTCDVSSGKCKRGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCMRGTGE---MLDWFEKEPALVRSENVVCPDGQSECKSGQTCCKLASGN-YGCCPIPKAVCCSDMKHCCPEGTTCDVSSGKCNRGSTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCSDGKHCCPENTKCEVSQGKCMRGTGEMLDWLKKEP 287 HSP 9 Score: 143.665 bits (361), Expect = 4.017e-33 Identity = 83/223 (37.22%), Postives = 117/223 (52.47%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----LKIPAISNL-----SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI 892
T G+Y CCP AVCCSD +HCCP+ T C+++ G C+ V+ + + SV+CPD Q +C DG +CC +A G + CCP AVCC+D +HCCP+NT C +S G C V+ + LK P + + +I CPD ++C TCC +G +GCC ++NAVCC CCP+ +C+ C K + N NR I
Sbjct: 718 TAAGNYGCCPLPKAVCCSDMKHCCPEGTTCDVSSGKCNRGNTYVMDWMDKQPAVVRAGSVMCPDGQHECKDGQTCCKLASGQWGCCPIPKAVCCNDGKHCCPENTKCEVSQGKCTRGTGLVLDWFEKLPALKRPGLVEMIKKDNTIVCPDQKTSCPDKNTCCKNKEGKFGCCAYNNAVCCKSGTYCCPKGYICDTLPEICRMPEAKEAW------KNTANRFI 934
BLAST of Granulin precursor vs. TrEMBL
Match: A0A087Y631 (Granulin b OS=Poecilia formosa OX=48698 PE=4 SV=2) HSP 1 Score: 274.633 bits (701), Expect = 6.432e-81 Identity = 160/376 (42.55%), Postives = 212/376 (56.38%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-------VLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF--------QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIK------ISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD--KNILLKFPIINFHP 1315
D S+ CCP AVCC DK+HCCP ++C+L C S +L+++ K P S SV+CPD +S C DG +CCV A G + CCPY A CC+D HCCP NT C+L+ G C S EV + +IPA S +ECPD + C G TCC +++GS+GCCP +AVCCSD + CCP T C+L TC S L IK + V+ V C +A C+DG TCCK PTG + CCP A CC D +HCCP TVCN+ TCDH + + + P+L E+ KC + C TCC +A+ +++CCP P AVCC DH HCCP+ T C+L ++CD + L P++ P
Sbjct: 123 DSSWGCCPLPKAVCCEDKRHCCPGGSKCDLVHLKCVSPSLESFPMLEKLPAKRSKDRPVSVAGSVICPDGESSCPDGYTCCVQASGQYGCCPYKEATCCNDHLHCCPSNTTCDLTHGVCKSGGNEV--PLTRRIPAASR-DVECPDKKTVCPDGTTCCQMTNGSFGCCPMPDAVCCSDHIHCCPSGTKCDLKHSTCVSFQDDAPLPIKKLPTMTNQGPAQSKVHAVPC-NASVACADGNTCCKSPTG-KWACCPLPQAVCCKDHLHCCPSGTVCNLAASTCDHPSGVALMPLLSNTPVFPLLTENDKCDESTSCPGNATCCKTASGAWACCPLPKAVCCDDHVHCCPHGTVCNLEAETCDDSSGVFLPLPLLTKVP 493 HSP 2 Score: 242.662 bits (618), Expect = 7.550e-69 Identity = 154/383 (40.21%), Postives = 204/383 (53.26%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNL------NKGTCDSVLKEVVTKIKLKVP--ASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDS-------KLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-DHQAEA-LEIKISPIL---------VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK-NILLKFPIINFHP*F 1321
T G YSCCP A CCSD HCC Q T C+L NK ++K + +K + S + +V+CPD +S+C D ++CC + D ++ CCP AVCC DK+HCCP + C+L C S L+++ K P S+ CPDG S+C G TCC+ + G YGCCP+ A CC+D L CCP NT C+L G C S + L + P V CPD K C DG TCC++ G S+GCCP A CCSD IHCCP T C++ TC Q +A L IK P + V ++ C C TCC S ++CCP P AVCC DH HCCP+ T C+L +CD + + P+++ P F
Sbjct: 37 TQGGYSCCPLPHAECCSDHLHCCYQGTVCDLVHQKCINKTVSLPMVKRLPSKQRSSASQLVSGVKAVMCPDHESECPDDTTCCQLYDSSWGCCPLPKAVCCEDKRHCCPGGSKCDLVHLKCVSPSLESFPMLEKLPAKRSKDRPVSVAGSVICPDGESSCPDGYTCCVQASGQYGCCPYKEATCCNDHLHCCPSNTTCDLTHGVCKSGGNEVPLTRRI--PAASRDVECPDKKTVCPDGTTCCQMTNG-SFGCCPMPDAVCCSDHIHCCPSGTKCDLKHSTCVSFQDDAPLPIKKLPTMTNQGPAQSKVHAVPCNASVACADGNTCCKSPTGKWACCPLPQAVCCKDHLHCCPSGTVCNLAASTCDHPSGVALMPLLSNTPVF 416 HSP 3 Score: 226.868 bits (577), Expect = 5.340e-63 Identity = 144/379 (37.99%), Postives = 192/379 (50.66%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPA--------SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSI--ECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK----------TKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH------QAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK---NILLKFPIIN 1306
T+GS+ CCP AVCCSD HCCP T+C+L TC S + IK K+P S + +V C C DG++CC G +ACCP AVCC D HCCP TVCNL+A TCD + + P L+ +C D S++C ATCC + G++ CCP AVCC D + CCP TVCNL A TCD TK P+L + Q +++C D + C TCC + +GCCP A CC D IHCCP C+ ++ +C + L P + +KC N S C TCC + + CCP AVCC DH HCCP +C++ TK+C+K N+ L +++
Sbjct: 280 TNGSFGCCPMPDAVCCSDHIHCCPSGTKCDLKHSTCVSFQDDAPLPIK-KLPTMTNQGPAQSKVHAVPCNASVACADGNTCCKSPTGKWACCPLPQAVCCKDHLHCCPSGTVCNLAASTCDHPSGVALMPLLSNTPVFPLLTENDKC-DESTSCPGNATCCKTASGAWACCPLPKAVCCDDHVHCCPHGTVCNLEAETCDDSSGVFLPLPLLTKVPALTSRVQQ----EKIMC-DRQTSCPKDATCCFMERTHKWGCCPLPEAVCCKDGIHCCPSGHTCDPHRSSCSSGPRRIPWSTKLGALAEPSALTDVKCDNTSSCATGTTCCKLPSGEWGCCPLLKAVCCPDHEHCCPQGYTCNMQTKTCEKKGYNLQLTTALLS 651 HSP 4 Score: 219.55 bits (558), Expect = 2.231e-60 Identity = 145/363 (39.94%), Postives = 185/363 (50.96%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNL--------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICP----DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA-----LEIKI----SPILVESIKCPNGSLCGQKGTCC-PSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
V G Y CCPY A CC+D HCCP NT C+L G C S EV + ++PA+S V CPD ++ C DG++CC M +G+F CCP AVCCSD HCCP T C+L TC S + +K K+P ++N ++ C + S C G TCC G + CCP AVCC D L CCP TVCNL A TCD + L+ PV ++ D C TCCK +G ++ CCP A CC D +HCCP TVCN+ TCD + L K+ S + E I C + C + TCC + CCP P AVCC D HCCP+ +C + SC
Sbjct: 204 VQASGQYGCCPYKEATCCNDHLHCCPSNTTCDLTHGVCKSGGNEV--PLTRRIPAAS-RDVECPDKKTVCPDGTTCCQMTNGSFGCCPMPDAVCCSDHIHCCPSGTKCDLKHSTCVSFQDDAPLPIK-KLPTMTNQGPAQSKVHAVPC-NASVACADGNTCCKSPTGKWACCPLPQAVCCKDHLHCCPSGTVCNLAASTCDHPSG--VALMPLLSNTPVFPLLTENDKCDESTSCPGNATCCKTASG-AWACCPLPKAVCCDDHVHCCPHGTVCNLEAETCDDSSGVFLPLPLLTKVPALTSRVQQEKIMCDRQTSCPKDATCCFMERTHKWGCCPLPEAVCCKDGIHCCPSGHTCDPHRSSC 558 HSP 5 Score: 207.994 bits (528), Expect = 4.621e-56 Identity = 134/369 (36.31%), Postives = 184/369 (49.86%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSV--VCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVV-TKMKLKIPAISNL----SIECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKK---PSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPI-------LVESIKCPNGS-------LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G ++CCP AVCC D HCCP T CNL TCD + + P +L+ C + + C ++CC A G +ACCP AVCC D HCCP TVCNL A TCD + + K+PA+++ I C D ++C ATCC + +GCCP AVCC D + CCP C+ +C S ++ + L +P+ + V C D C+ G TCCKLP+G+ +GCCP A CC D HCCPQ CN+ TC+ + L++ + + L E+++ N LC TCC + +SCCP+ A CC+D HCCP SC L +SC L +
Sbjct: 365 GKWACCPLPQAVCCKDHLHCCPSGTVCNLAASTCDHPSGVALMPLLSNTPVFPLLTENDKCDESTSCPGNATCCKTASGAWACCPLPKAVCCDDHVHCCPHGTVCNLEAETCDDSSGVFLPLPLLTKVPALTSRVQQEKIMC-DRQTSCPKDATCCFMERTHKWGCCPLPEAVCCKDGIHCCPSGHTCDPHRSSCSSGPRRIPWSTKLGALAEPSALTDVKC-DNTSSCATGTTCCKLPSGE-WGCCPLLKAVCCPDHEHCCPQGYTCNMQTKTCEKKGYNLQLTTALLSKVEQSALREAVEETNVQCDSTGVFLCPANSTCCKISATEWSCCPFAKAECCADMKHCCPVGYSCDLKARSCTPQTRLTW 730 HSP 6 Score: 199.519 bits (506), Expect = 5.690e-53 Identity = 120/299 (40.13%), Postives = 157/299 (52.51%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT--------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI-KISPILVESIKCPN-GSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
+++CP+ S C D +CC G ++CCP A CCSD HCC Q TVC+L C +K + +L S+ S + CPD S C TCC L D S+GCCP AVCC DK CCP + C+L C S + K P+ K + VICPD + C DG TCC +G YGCCPY ATCC+D +HCCP NT C++ G C + + + P ++CP+ ++C TCC N S+ CCP P+AVCCSDH HCCP+ T C L +C
Sbjct: 18 ALICPNGSMCGDEDTCCKNTQGGYSCCPLPHAECCSDHLHCCYQGTVCDLVHQKCINKTVSLPMVKRLPSKQRSSASQLVSGVKAVMCPDHESECPDDTTCCQLYDSSWGCCPLPKAVCCEDKRHCCPGGSKCDLVHLKCVSPSLESFPMLEKLPAKRSKDRPVSVAGSVICPDGESSCPDGYTCCVQASGQ-YGCCPYKEATCCNDHLHCCPSNTTCDLTHGVCKSGGNEVPLTRRIPAASRDVECPDKKTVCPDGTTCCQMTNGSFGCCPMPDAVCCSDHIHCCPSGTKCDLKHSTC 315 HSP 7 Score: 153.68 bits (387), Expect = 1.224e-36 Identity = 97/290 (33.45%), Postives = 138/290 (47.59%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-----VLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGN-FACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNL--SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSL---LIKTFQPNPVNRVICPD-------AKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAE 1069
T G+++CCP AVCC D HCCP T CNL TCD + ++TK+ ++C Q+ C ++CC M + + CCP AVCC D HCCP C+ +C S + + KL A + ++C D +S+C TG TCC L G +GCCP AVCC D CCPQ CN+ TC+ K L L+ + + + + + C TCCK+ + + CCP++ A CC+D HCCP C++ +C Q
Sbjct: 440 TASGAWACCPLPKAVCCDDHVHCCPHGTVCNLEAETCDDSSGVFLPLPLLTKVPALTSRVQQEKIMCDRQTSCPKDATCCFMERTHKWGCCPLPEAVCCKDGIHCCPSGHTCDPHRSSCSSGPRRIPWSTKLGALAEPSALTDVKC-DNTSSCATGTTCCKLPSGEWGCCPLLKAVCCPDHEHCCPQGYTCNMQTKTCEKKGYNLQLTTALLSKVEQSALREAVEETNVQCDSTGVFLCPANSTCCKI-SATEWSCCPFAKAECCADMKHCCPVGYSCDLKARSCTPQTR 727 HSP 8 Score: 85.8853 bits (211), Expect = 4.332e-14 Identity = 58/156 (37.18%), Postives = 78/156 (50.00%), Query Frame = 2
Query: 887 VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI----------KISPIL--VESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
+ICP+ C D TCCK G Y CCP A CCSD +HCC Q TVC++ C ++ +L + S ++ V+++ CP+ S C TCC + S+ CCP P AVCC D HCCP + C L C L FP++ P
Sbjct: 19 LICPNGSM-CGDEDTCCKNTQG-GYSCCPLPHAECCSDHLHCCYQGTVCDLVHQKCINKTVSLPMVKRLPSKQRSSASQLVSGVKAVMCPDHESECPDDTTCCQLYDSSWGCCPLPKAVCCEDKRHCCPGGSKCDLVHLKCVSPSLESFPMLEKLP 172 HSP 9 Score: 65.0846 bits (157), Expect = 1.772e-7 Identity = 33/73 (45.21%), Postives = 43/73 (58.90%), Query Frame = 2
Query: 1097 LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
L ++ CPNGS+CG + TCC + YSCCP P+A CCSDH HCC T C L + C N + P++ P
Sbjct: 15 LSAALICPNGSMCGDEDTCCKNTQGGYSCCPLPHAECCSDHLHCCYQGTVCDLVHQKC-INKTVSLPMVKRLP 86
BLAST of Granulin precursor vs. TrEMBL
Match: A0A2G8L0S4 (Putative fibrillin-1 isoform X4 (Fragment) OS=Stichopus japonicus OX=307972 GN=BSL78_09258 PE=4 SV=1) HSP 1 Score: 270.396 bits (690), Expect = 8.065e-81 Identity = 152/347 (43.80%), Postives = 204/347 (58.79%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCP-DQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--KISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+ G Y CCP +AVCCSD HCCP C+ ++GTC S E++ + K A+++ +V CP QS+C DG++CC+++ G + CCP +AVCCSD HCCP C++S GTC S+ E++ + K A ++ CP G S C G TCCLLS G YGCCP NAVCCSD L CCP C++ GTC ++ + KT + V V CP + +C DG TCC L +G YGCCP A CCSD +HCCP C+I+ GTC +E + K + ++ CP G S C TCC ++ Y CCP PNAVCCSDH HCCPN +C ++ +C +
Sbjct: 165 ILATGRYGCCPLRNAVCCSDHLHCCPNGYTCDASQGTC-SQGSEIMNWFE-KTEATTLGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTC-SQGSEIMAWFE-KTEATPVGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSGGTCSQGSEIMAWFEKT-EATTVGTVTCPGGQSECPDGNTCCLLSSGQ-YGCCPLPNAVCCSDHLHCCPNGYTCDISGGTCSQGSEIMNWFEKTEATTLGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTCSQG 505 HSP 2 Score: 264.618 bits (675), Expect = 1.186e-78 Identity = 151/355 (42.54%), Postives = 207/355 (58.31%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--KISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPN--------AVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+ + G Y CCP +AVCCSD HCCP C++++GTC S E++ + K A+ + +V CP QS+C DG++CC+++ G + CCP +AVCCSD HCCP C++S GTC S+ E++ + K A + ++ CP G S C G TCCLLS G YGCCP NAVCCSD L CCP C++ GTC ++ + KT + + V CP + +C DG TCC L +G YGCCP A CCSD +HCCP C++++GTC +E + K V ++ CP G S C TCC ++ Y CCP PN AVCCSDH HCCPN +C ++ +C +
Sbjct: 240 LLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTC-SQGSEIMAWFE-KTEATPVGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSGGTC-SQGSEIMAWFE-KTEATTVGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDISGGTCSQGSEIMNWFEKT-EATTLGTVTCPGGQSECPDGNTCCLLSSGQ-YGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTCSQGSEIMNWFEKTEATTVGTVTCPGGQSECPDGSTCCLLSSGQYGCCPLPNCKILLILQAVCCSDHLHCCPNGYTCDVSQGTCSQG 588 HSP 3 Score: 253.062 bits (645), Expect = 3.128e-74 Identity = 151/361 (41.83%), Postives = 198/361 (54.85%), Query Frame = 2
Query: 260 DGSYSCCP---YSSAVCCSDK--------------QHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--KISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
DG Y CCP Y+ CS+ HCCP+ C+ ++ C V K A + SV+CPD+ S+C DGS+CC++A G + CCP +AVCCSD HCCP C+ S GTC S+ E++ + K A + ++ CP G S C G TCCLLS G YGCCP NAVCCSD L CCP C++ GTC ++ + KT + PV V CP + +C DG TCC L +G YGCCP A CCSD +HCCP C+++ GTC +E + K V ++ CP G S C TCC ++ Y CCP PNAVCCSDH HCCPN +C ++ +C +
Sbjct: 76 DGKYGCCPCPKYTCTALCSEDICSSSLFVKAECCDTHCCPEGFSCDGDQ--CSKEEGNVFVAGVSKTEAVIVESVMCPDRRSECPDGSTCCILATGRYGCCPLRNAVCCSDHLHCCPNGYTCDASQGTC-SQGSEIMNWFE-KTEATTLGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTCSQGSEIMAWFEKT-EATPVGTVTCPGGQSECPDGNTCCLLSSGQ-YGCCPLPNAVCCSDHLHCCPNGYTCDVSGGTCSQGSEIMAWFEKTEATTVGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDISGGTCSQG 430 HSP 4 Score: 222.246 bits (565), Expect = 1.964e-62 Identity = 138/356 (38.76%), Postives = 187/356 (52.53%), Query Frame = 2
Query: 293 AVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCP---YSSAVCCSD--------------KQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--KISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN--ILLKF 1294
AVCCSD +HCCP C++++GTC S E + + +V +V C + C DG++CC ADG + CCP Y+ CS+ HCCP+ C+ C + V K A+ S+ CPD S C G+TCC+L+ G YGCCP NAVCCSD L CCP C+ GTC ++ + KT + + V CP + +C DG TCC L +G YGCCP A CCSD +HCCP C++++GTC +E + K V ++ CP G S C TCC ++ Y CCP PNAVCCSDH HCCPN +C ++ +C + I+ F
Sbjct: 16 AVCCSDGEHCCPSGYTCDVSEGTCSSE-SEKLPWFEKEVAK----NVHCGGKYYCKDGNTCCPQADGKYGCCPCPKYTCTALCSEDICSSSLFVKAECCDTHCCPEGFSCD--GDQCSKEEGNVFVAGVSKTEAVIVESVMCPDRRSECPDGSTCCILATGRYGCCPLRNAVCCSDHLHCCPNGYTCDASQGTCSQGSEIMNWFEKT-EATTLGTVTCPGGQSECPDGNTCCLLSSGQ-YGCCPLPNAVCCSDHLHCCPNGYTCDVSQGTCSQGSEIMAWFEKTEATPVGTVTCPGGQSECPDGNTCCLLSSGQYGCCPLPNAVCCSDHLHCCPNGYTCDVSGGTCSQGSEIMAWF 362
BLAST of Granulin precursor vs. TrEMBL
Match: A0A4W5RTY1 (Granulin b OS=Hucho hucho OX=62062 PE=4 SV=1) HSP 1 Score: 273.863 bits (699), Expect = 4.753e-80 Identity = 163/375 (43.47%), Postives = 210/375 (56.00%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK-------TKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH--QAEALEIKISPILVESIKCPNGSL----------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP*FN 1324
+GS+ CCP + AVCC DK HCCP+ T+C+L C S E ++ K PA +V CP +S+C DG++CC++ G++ CCPY AVCC+DK HCCP NT C+L C S T + K PAI N ++CPD S+C TCCLL DGSYGCCP +AVCC D L CCP+ T C+L GTC SK K P+ L TF + VN V C D+ C+DG TCCKL G+ + CCP A CC D +HCCP T+CN+ + TCD AL + + S P L C K CC + +++CC PNAVCC+DH HCCP+ T C+L +CD P +N P F
Sbjct: 119 EGSWGCCPLAKAVCCEDKMHCCPEGTKCDLAHTKCVSPTLETFA-MREKGPAMKKQTVTCPGGKSRCPDGTTCCLLTSGDYGCCPYLEAVCCTDKLHCCPGNTTCDLERDMCTSP--NTQTPLAKKTPAIPN-DVDCPDKVSSCPDETTCCLLGDGSYGCCPMPSAVCCPDHLHCCPEGTTCDLEQGTCVSKHGQTSMAVKFPATLTTTFLQSTVNGVPCNDS-VTCADGTTCCKLLNGE-WACCPLPKAVCCDDHLHCCPHGTICNLEESTCDDPSSGSALVPMLDKVPAFSYVSPEEPLPNSICDRSTSCPGKSMCCKTTTGNWACCLLPNAVCCNDHIHCCPDGTVCNLEASTCDDPSGFNVPWVNKVPAFT 487 HSP 2 Score: 260.766 bits (665), Expect = 3.612e-75 Identity = 156/377 (41.38%), Postives = 206/377 (54.64%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS---------VVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPIL--------VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD--KNILLKFPIINFHP*FNY 1327
G Y CCP +A CCSD HCC + T C+L C + K V +VPA + S V+CPD +S+C + ++CC + +G++ CCP + AVCC DK HCCP+ T C+L+ C S E M+ K PA+ ++ CP G S C G TCCLL+ G YGCCP+ AVCC+DKL CCP NT C+L C S + L KT P N V CPD C D TCC L G SYGCCP +A CC D +HCCP+ T C++ +GTC H ++ +K L V + C + C TCC N ++CCP P AVCC DH HCCP+ T C+L +CD + P+++ P F+Y
Sbjct: 36 GGYGCCPLPNAECCSDHLHCCYEGTVCDLVHSKC--LNKTVSLPWVRRVPAHHLFSPLMVEGVRAVICPDGESECPEDTTCCQLPEGSWGCCPLAKAVCCEDKMHCCPEGTKCDLAHTKCVSPTLETFA-MREKGPAMKKQTVTCPGGKSRCPDGTTCCLLTSGDYGCCPYLEAVCCTDKLHCCPGNTTCDLERDMCTSPNTQTPLAKKT--PAIPNDVDCPDKVSSCPDETTCCLLGDG-SYGCCPMPSAVCCPDHLHCCPEGTTCDLEQGTCVSKHGQTSMAVKFPATLTTTFLQSTVNGVPCNDSVTCADGTTCCKLLNGEWACCPLPKAVCCDDHLHCCPHGTICNLEESTCDDPSSGSALVPMLDKVPAFSY 406 HSP 3 Score: 238.424 bits (607), Expect = 9.762e-67 Identity = 154/379 (40.63%), Postives = 199/379 (52.51%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS--------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL-----IKTFQ----PNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD----------HQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPII 1303
+ T G Y CCPY AVCC+DK HCCP NT C+L + C S T + K PA V CPD+ S C D ++CC++ DG++ CCP SAVCC D HCCP+ T C+L GTC SK + T M +K PA + + C D S TC G TCC L +G + CCP AVCC D L CCP T+CNL TCD + +L+ + F P+ IC D C CCK TG+ + CC A CC+D IHCCP TVCN+ TCD ++ A + +P+ E KC ++C TCC + ++CCP P AVCC DH HCCP +C++ ++CDK L P I
Sbjct: 192 LLTSGDYGCCPYLEAVCCTDKLHCCPGNTTCDLERDMCTS--PNTQTPLAKKTPAIPN-DVDCPDKVSSCPDETTCCLLGDGSYGCCPMPSAVCCPDHLHCCPEGTTCDLEQGTCVSKHGQ--TSMAVKFPATLTTTFLQSTVNGVPCND-SVTCADGTTCCKLLNGEWACCPLPKAVCCDDHLHCCPHGTICNLEESTCDDPSSGSALVPMLDKVPAFSYVSPEEPLPNSIC-DRSTSCPGKSMCCKTTTGN-WACCLLPNAVCCNDHIHCCPDGTVCNLEASTCDDPSGFNVPWVNKVPAFTTQ-APLATE--KCDKQTMCPGGTTCCRQNSGQWACCPLPLAVCCDDHEHCCPKGYTCNVADQTCDKAGTLSLPWI 559 HSP 4 Score: 219.164 bits (557), Expect = 1.060e-59 Identity = 128/306 (41.83%), Postives = 170/306 (55.56%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPA---ISNLSIE------CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH-QAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC-----DKNILLKFP 1297
+++CPD CDDG++CC G + CCP +A CCSD HCC + TVC+L C +K V ++PA S L +E CPDG S C TCC L +GS+GCCP + AVCC DK+ CCP+ T C+L C S T + + + V CP K +C DG TCC L +GD YGCCPY A CC+DK+HCCP NT C++ + C + K +P + + CP+ S C + TCC + SY CCP P+AVCC DH HCCP T+C L +C ++ +KFP
Sbjct: 15 ALICPDGGMCDDGNTCCKNPSGGYGCCPLPNAECCSDHLHCCYEGTVCDLVHSKCLNKT--VSLPWVRRVPAHHLFSPLMVEGVRAVICPDGESECPEDTTCCQLPEGSWGCCPLAKAVCCEDKMHCCPEGTKCDLAHTKCVSPTLETFAMREKGPAMKKQTVTCPGGKSRCPDGTTCCLLTSGD-YGCCPYLEAVCCTDKLHCCPGNTTCDLERDMCTSPNTQTPLAKKTPAIPNDVDCPDKVSSCPDETTCCLLGDGSYGCCPMPSAVCCPDHLHCCPEGTTCDLEQGTCVSKHGQTSMAVKFP 317 HSP 5 Score: 218.394 bits (555), Expect = 1.739e-59 Identity = 147/384 (38.28%), Postives = 196/384 (51.04%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPA--------SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDS-KLKEVVTKMKLKIPAISNLSIECP------DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT--------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI----KISPILVESI--------------KCPNGSLCGQKGTCC-PSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
DGSY CCP SAVCC D HCCP+ T C+L +GTC V K T + +K PA S++ V C D C DG++CC + +G +ACCP AVCC D HCCP T+CNL TCD + M K+PA S +S E P D S++C + CC + G++ CC NAVCC+D + CCP TVCNL A TCD + K P+ + P+ C D + C G TCC+ +G + CCP A CC D HCCP+ CN+ TCD +A L + K+ +L+ + C + C + TCC + + CCP P AVCC++ HCCP+ SC ++ SC K L
Sbjct: 269 DGSYGCCPMPSAVCCPDHLHCCPEGTTCDLEQGTC--VSKHGQTSMAVKFPATLTTTFLQSTVNGVPCNDSVTCADGTTCCKLLNGEWACCPLPKAVCCDDHLHCCPHGTICNLEESTCDDPSSGSALVPMLDKVPAFSYVSPEEPLPNSICDRSTSCPGKSMCCKTTTGNWACCLLPNAVCCNDHIHCCPDGTVCNLEASTCDDPSGFNVPWVNKVPAFTTQA----PLATEKC-DKQTMCPGGTTCCRQNSG-QWACCPLPLAVCCDDHEHCCPKGYTCNVADQTCD-KAGTLSLPWIPKVPALLLHGVLPQASAHAFPPTKNMCDPHTSCPKYTTCCFMNKAGKWGCCPLPKAVCCANGDHCCPSGYSCDVHKTSCTKGRL 643 HSP 6 Score: 207.994 bits (528), Expect = 1.019e-55 Identity = 135/377 (35.81%), Postives = 181/377 (48.01%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-VLKEVVTKIKLKVPASSILS-------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISN---LSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT--------KKPSLLIKTFQPNPVNRVICP-----DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL------EIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK---NILLK 1291
+G ++CCP AVCC D HCCP T CNL + TCD + + KVPA S +S +C + C S CC GN+ACC +AVCC+D HCCP TVCNL A TCD V + K+PA + L+ E D + C G TCC + G + CCP AVCC D CCP+ CN+ TCD K P+LL+ P P D C TCC + +GCCP A CC++ HCCP C+++K +C + + +++ +KC + S C TCC + CCP AVCC+DH HCCP SC++ T +C+K +LL+
Sbjct: 351 NGEWACCPLPKAVCCDDHLHCCPHGTICNLEESTCDDPSSGSALVPMLDKVPAFSYVSPEEPLPNSICDRSTSCPGKSMCCKTTTGNWACCLLPNAVCCNDHIHCCPDGTVCNLEASTCDDPSGFNVPWVN-KVPAFTTQAPLATEKCDKQTMCPGGTTCCRQNSGQWACCPLPLAVCCDDHEHCCPKGYTCNVADQTCDKAGTLSLPWIPKVPALLLHGVLPQASAHAFPPTKNMCDPHTSCPKYTTCCFMNKAGKWGCCPLPKAVCCANGDHCCPSGYSCDVHKTSCTKGRLTIPWYRKEKALTEGAMLKDVKCDDKSSCASGATCCKLPTGEWGCCPLVKAVCCTDHEHCCPQGYSCNMQTGTCEKLVEGVLLR 726 HSP 7 Score: 197.593 bits (501), Expect = 5.278e-52 Identity = 138/390 (35.38%), Postives = 194/390 (49.74%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPA----SSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSS--------------TCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNP-------VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPI--LVES----------IKCPNGSL--CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPII 1303
TT G+++CC +AVCC+D HCCP T CNL TCD V + KVPA + + + C Q+ C G++CC G +ACCP AVCC D +HCCP+ CN++ TCD K + K+PA+ L P S+ +C TCC ++ G +GCCP AVCC++ CCP C++ +C K L I ++ + V C D K C+ G TCCKLPTG+ +GCCP A CC+D HCCPQ CN+ GTC+ E + ++ P+ + ES +KC + C + TCC ++ +SCCP P A+CC+D HCCP +C L C + L + +
Sbjct: 433 TTTGNWACCLLPNAVCCNDHIHCCPDGTVCNLEASTCDDPSGFNVPWVN-KVPAFTTQAPLATEKCDKQTMCPGGTTCCRQNSGQWACCPLPLAVCCDDHEHCCPKGYTCNVADQTCD-KAGTLSLPWIPKVPALL-LHGVLPQASAHAFPPTKNMCDPHTSCPKYTTCCFMNKAGKWGCCPLPKAVCCANGDHCCPSGYSCDVHKTSC----TKGRLTIPWYRKEKALTEGAMLKDVKC-DDKSSCASGATCCKLPTGE-WGCCPLVKAVCCTDHEHCCPQGYSCNMQTGTCEKLVEGVLLRTVPLTKVAESQQRASEMGIDVKCDSTGEYSCPKLETCCKTSPTEWSCCPAPKAICCADTRHCCPMGYTCDLERGGCSQQAELTWDMF 813 HSP 8 Score: 99.7525 bits (247), Expect = 1.396e-18 Identity = 73/219 (33.33%), Postives = 99/219 (45.21%), Query Frame = 2
Query: 743 FSNAVCCSDKLSCCPQNTVCNLPAG-------------------------------TCDSKTKKPSLLIKT-----FQP---NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---DHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
++A+ C D C NT C P+G C +KT + + F P V VICPD + +C + TCC+LP G S+GCCP + A CC DK+HCCP+ T C++ C + A+ K + +++ CP G S C TCC + Y CCPY AVCC+D HCCP NT+C L C
Sbjct: 12 LTSALICPDGGMCDDGNTCCKNPSGGYGCCPLPNAECCSDHLHCCYEGTVCDLVHSKCLNKTVSLPWVRRVPAHHLFSPLMVEGVRAVICPDGESECPEDTTCCQLPEG-SWGCCPLAKAVCCEDKMHCCPEGTKCDLAHTKCVSPTLETFAMREKGPAMKKQTVTCPGGKSRCPDGTTCCLLTSGDYGCCPYLEAVCCTDKLHCCPGNTTCDLERDMC 229
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 221.476 bits (563), Expect = 1.831e-64 Identity = 152/380 (40.00%), Postives = 194/380 (51.05%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS--VLKEVVTKIKLKVPAS-SILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLK--IPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPS--LLIKTFQPNPVNRVICPDAKYQCSD------GQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ--AEALEIKISPIL---------------------VESI-KCPNGSLCGQKGTCCPSAN-DSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
+GSY CCP AVCCSD HCCPQ T C+L C S VL+ V T + V ++ SV C + C+ GS+CC DG++ACCP AVCC D HCCPQ TVC S + L +V ++ K I + + E D ++TC GATCC + G++GCCP AVCC D L CCP+NTVCN+ AGTCDS + L + + P + P A QC + G TCC+ +G ++ CCP A CC D HCCPQ C+I +CDH L K P L VES KC + C + TCC + + CCP P AVCC D HCCPN+ +C +C K L
Sbjct: 208 NGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSL--PPANDQCDETSACPTGTTCCRQKSG-AWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHL 584 HSP 2 Score: 221.476 bits (563), Expect = 2.317e-64 Identity = 151/394 (38.32%), Postives = 195/394 (49.49%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC--DSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS-----------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICP------DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVE-----SIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-----LLKFPIINFHP 1315
T G Y CCP +A CCSD HCC + T C+L C + + V ++ ++ P S +VVCPDQ S+C D ++CC + DG++ CCP +AVCC DK HCCPQ T C+L C S + V ++ K PA ++CPD S C TCC L +GSYGCCP AVCCSD + CCPQ T C+L C S L T +PV RV + C G TCCK GD + CCP A CC D IHCCPQ TVC + TC A+ L ++ P + + KC + C + TCC + ++ CCP P AVCC DH HCCP NT C++ +CD L P + P
Sbjct: 35 TPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQP-SLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSV-PLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSN---VLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGD-WACCPLPEAVCCEDHIHCCPQGTVC--ARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTP 420 HSP 3 Score: 211.075 bits (536), Expect = 1.213e-60 Identity = 129/328 (39.33%), Postives = 174/328 (53.05%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLK--EVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNP---------VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPIL-----VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNT--KSCDKNILLKF----PIINFHP 1315
+++CPD CDDG++CC G + CCP +A CCSD HCC + T+C+L C +K + V ++ ++ P++ ++ CPD S C TCC L DGS+GCCP NAVCC DK+ CCPQ T C+LP C S + K P+ ++ N V CPD C D TCC L G SYGCCP A CCSD +HCCPQ T C++ C + E + +SP++ VES+ C C TCC + + ++CCP P AVCC DH HCCP T C+ +T S D L+++ P IN P
Sbjct: 17 ALICPDGGMCDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQ-AVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNG-SYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVP 342 HSP 4 Score: 206.068 bits (523), Expect = 7.907e-59 Identity = 136/395 (34.43%), Postives = 185/395 (46.84%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCD------DGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL----KIPAIS--NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK--------TKKPSLLIKTFQPNPVNRVICP------------DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
T DG ++CCP AVCC D HCCPQ T C + TC +++T ++ +I V P +CD G++CC M G + CCP AVCC D HCCP+NTVCN++AGTCDS ++ + K PA S + +C D +S C TG TCC G++ CCP AVCC D CCPQ C++ +CD K+P+L T Q N + I DA+ C TCC + + +GCCP A CC D HCCP + C+ K TC +A+ + V ++C + + C TCC + CCP+ AVCC DH HCCP +C L +C + L +
Sbjct: 285 TKDGDWACCPLPEAVCCEDHIHCCPQGTVCA--RSTC-VASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTL 675 HSP 5 Score: 197.593 bits (501), Expect = 1.040e-55 Identity = 126/382 (32.98%), Postives = 183/382 (47.91%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL----KVPASSI--LSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP--------------------DGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILV---ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G++ CCP AVCC D HCCP+NT CN+ GTCDS ++ + K PA+S+ + C + S C G++CC G +ACCP AVCC D +HCCPQ C++ +CD + K K PA+ +++ + D ++C TCC + +GCCP AVCC D CCP + C+ TC K ++ + + + V V C D+ +C G TCC+L TG +GCCP+ A CC D +HCCP C++ GTC + + ++ + E + C S C + TCC ++ ++CCP+ AVCC D HCCP C K C + L +
Sbjct: 367 GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFK-KQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTG-QWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVHTHTKEQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATSLTW 745
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grnb (granulins-like [Source:NCBI gene;Acc:103046308]) HSP 1 Score: 221.476 bits (563), Expect = 1.831e-64 Identity = 152/380 (40.00%), Postives = 194/380 (51.05%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS--VLKEVVTKIKLKVPAS-SILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLK--IPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPS--LLIKTFQPNPVNRVICPDAKYQCSD------GQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ--AEALEIKISPIL---------------------VESI-KCPNGSLCGQKGTCCPSAN-DSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
+GSY CCP AVCCSD HCCPQ T C+L C S VL+ V T + V ++ SV C + C+ GS+CC DG++ACCP AVCC D HCCPQ TVC S + L +V ++ K I + + E D ++TC GATCC + G++GCCP AVCC D L CCP+NTVCN+ AGTCDS + L + + P + P A QC + G TCC+ +G ++ CCP A CC D HCCPQ C+I +CDH L K P L VES KC + C + TCC + + CCP P AVCC D HCCPN+ +C +C K L
Sbjct: 208 NGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSL--PPANDQCDETSACPTGTTCCRQKSG-AWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHL 584 HSP 2 Score: 221.476 bits (563), Expect = 2.317e-64 Identity = 151/394 (38.32%), Postives = 195/394 (49.49%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC--DSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS-----------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICP------DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL----EIKISPILVE-----SIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-----LLKFPIINFHP 1315
T G Y CCP +A CCSD HCC + T C+L C + + V ++ ++ P S +VVCPDQ S+C D ++CC + DG++ CCP +AVCC DK HCCPQ T C+L C S + V ++ K PA ++CPD S C TCC L +GSYGCCP AVCCSD + CCPQ T C+L C S L T +PV RV + C G TCCK GD + CCP A CC D IHCCPQ TVC + TC A+ L ++ P + + KC + C + TCC + ++ CCP P AVCC DH HCCP NT C++ +CD L P + P
Sbjct: 35 TPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQP-SLPQAVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSV-PLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNGSYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSN---VLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGD-WACCPLPEAVCCEDHIHCCPQGTVC--ARSTCVASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTP 420 HSP 3 Score: 211.075 bits (536), Expect = 1.213e-60 Identity = 129/328 (39.33%), Postives = 174/328 (53.05%), Query Frame = 2
Query: 428 SVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLK--EVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNP---------VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--DHQAEALEIKISPIL-----VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNT--KSCDKNILLKF----PIINFHP 1315
+++CPD CDDG++CC G + CCP +A CCSD HCC + T+C+L C +K + V ++ ++ P++ ++ CPD S C TCC L DGS+GCCP NAVCC DK+ CCPQ T C+LP C S + K P+ ++ N V CPD C D TCC L G SYGCCP A CCSD +HCCPQ T C++ C + E + +SP++ VES+ C C TCC + + ++CCP P AVCC DH HCCP T C+ +T S D L+++ P IN P
Sbjct: 17 ALICPDGGMCDDGNTCCQTPSGGYGCCPLPNAECCSDHLHCCHEGTLCDLEHSKCVNKTHTLDWVMRVPIRQPSLPQ-AVVCPDQESECPDDTTCCQLPDGSWGCCPMPNAVCCEDKMHCCPQGTTCDLPHSKCVSSMLGSVPLRRKFPAYKRAEWKARKDSNGLDLADSNDVDCPDKVSFCPDDTTCCPLGNG-SYGCCPMPKAVCCSDHVHCCPQGTSCDLAHSKCVSSNVLEPVTTNLSPVVRVKVTVESVPCNESVACESGSTCCKTKDGDWACCPLPEAVCCEDHIHCCPQGTVCARSTCVASADLLTLVRWVEKKPTINRVP 342 HSP 4 Score: 206.068 bits (523), Expect = 7.907e-59 Identity = 136/395 (34.43%), Postives = 185/395 (46.84%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCD------DGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL----KIPAIS--NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK--------TKKPSLLIKTFQPNPVNRVICP------------DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------DHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINF 1309
T DG ++CCP AVCC D HCCPQ T C + TC +++T ++ +I V P +CD G++CC M G + CCP AVCC D HCCP+NTVCN++AGTCDS ++ + K PA S + +C D +S C TG TCC G++ CCP AVCC D CCPQ C++ +CD K+P+L T Q N + I DA+ C TCC + + +GCCP A CC D HCCP + C+ K TC +A+ + V ++C + + C TCC + CCP+ AVCC DH HCCP +C L +C + L +
Sbjct: 285 TKDGDWACCPLPEAVCCEDHIHCCPQGTVCA--RSTC-VASADLLTLVRWVEKKPTINRVPQPANEKCDPTTTCPKGATCCKMESGTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQC-DETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFKKQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQCDSTARCPTGSTCCRLTTGQWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTL 675 HSP 5 Score: 197.593 bits (501), Expect = 1.040e-55 Identity = 126/382 (32.98%), Postives = 183/382 (47.91%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL----KVPASSI--LSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP--------------------DGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILV---ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G++ CCP AVCC D HCCP+NT CN+ GTCDS ++ + K PA+S+ + C + S C G++CC G +ACCP AVCC D +HCCPQ C++ +CD + K K PA+ +++ + D ++C TCC + +GCCP AVCC D CCP + C+ TC K ++ + + + V V C D+ +C G TCC+L TG +GCCP+ A CC D +HCCP C++ GTC + + ++ + E + C S C + TCC ++ ++CCP+ AVCC D HCCP C K C + L +
Sbjct: 367 GTWGCCPLPQAVCCEDHLHCCPRNTVCNVAAGTCDSAFSGTAGQLSVPWVRKTPATSLPPANDQCDETSACPTGTTCCRQKSGAWACCPLPQAVCCDDHEHCCPQGYKCDIIHESCDHPFLPSMLWFK-KQPALQSMTQQSNANAKAIAESAVADVESKHKCDAQTSCPRDNTCCYMKKFNKWGCCPLPEAVCCGDGDHCCPNSYTCDTTKNTCLKGHLEIPWFKKQEAKVTQGSEVAHGVADVQC-DSTARCPTGSTCCRLTTG-QWGCCPHVQAVCCDDHVHCCPAGYTCDLQAGTCLRTSTLHTVALTLVHTHTKEQVMCDATSHCTESQTCCKLSDTEWACCPFKQAVCCKDMKHCCPVGYVCDPKAKGCARATSLTW 745
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 219.164 bits (557), Expect = 1.260e-63 Identity = 143/379 (37.73%), Postives = 191/379 (50.40%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS------VLKEVVTK--IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLIKTFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKIS-PI----------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI 1282
+G +SCCP AVCC D HCCP +CNL G C+ LK+V ++ + K+P + + +V C D + C D ++CC ++G + CCP AVCC D HCCP CNL+AGTC+ V KL K+P ++ C D ++ C TCC S+G + CCP AVCC D CCP CN+ AGTC+ + K PS I + V P +A + C D TCCK G + CCP A CC D +HCCP CN+ TCD + + LE K S PI ++ C + C TCC N +SCCP+P VCC D HCCP++ +C+L T +CDKN
Sbjct: 307 EGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCND-TAACPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKG-GWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNT 683 HSP 2 Score: 200.675 bits (509), Expect = 8.736e-57 Identity = 134/410 (32.68%), Postives = 183/410 (44.63%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC-----------------------------------------DSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTK--MKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKY---------QCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI------------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
TTDG + CCP AVCC+DK HCCP+N+ C++N C + LK T S++ +V C D C DG++CC +G ++CCP AVCC D HCCP CNL AG C+ LK+V ++ + K+P ++ C D ++ C TCC S+G + CCP AVCC D + CCP CNL AGTC+ + + K P+ P+ K C D TCCK G+ + CCP A CC D HCCP CN+ GTC+ + ++ K+ + + C C + TCC + +SCCP P AVCC D HCCP+ C+L +CD
Sbjct: 188 TTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCND-TAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWM-KKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGE-WKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCD 594 HSP 3 Score: 176.792 bits (447), Expect = 2.797e-48 Identity = 124/410 (30.24%), Postives = 178/410 (43.41%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS---------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-----------------------QP-------------------NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL------------EIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
V + G Y+CCP+ CC D HCCP C + C + + + PA + L+ ++CPD S C SC + D + CCP + + C+D+ HCCP + C+ + +C K + V+ CPDG S C TCC +DG +GCCP AVCC+DK+ CCP+N+VC++ + C S T K + F QP + V V C D C+DG TCCK G + CCP A CC D +HCCP CN+ G C+ + ++ E K+ V ++ C + + C + TCC + + CCP P AVCC D HCCP+ C+L +C+
Sbjct: 38 VLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSSCPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPVM----------------CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPC-DDTVACADGTTCCKNGEG-GWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCE 426 HSP 4 Score: 175.252 bits (443), Expect = 1.078e-47 Identity = 124/364 (34.07%), Postives = 169/364 (46.43%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL--------KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLI----KTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKS 1267
++G + CCP AVCC D HCCP +CNL GTC+ V KL K+P + + +V C D + C D ++CC ++G + CCP AVCC D QHCCP CN++AGTC+ V K+ K+P + + C + + C+ +TCC G + CCP AVCC D L CCP CNL A TCD + KKPS I K V C D+ + C TCCK + + CCP+ CC D+IHCCP + CN+ TCD ++ + +K +K AN+ V C DH H CP+ T+C K+
Sbjct: 390 SEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSC-DSSHFCPASSTCCKDVNAE-WSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVP------MAGPVKASGPPPPEKKKPKENKANE---------PVKCDDH-HSCPDQTTCCFMNKT 734 HSP 5 Score: 140.198 bits (352), Expect = 1.866e-35 Identity = 110/363 (30.30%), Postives = 163/363 (44.90%), Query Frame = 2
Query: 383 VVTKIKLKVPASSILSVV----CPDQSQCDDGSSCC-VMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS---------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGD-SYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI----------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++T +KL V A ++L +V CP+Q C D +CC V++ G + CCP+ CC D HCCP +C + C + + + PA + L+ I CPD SS C +C L+D YGCCP + + C+D+ CCP + C+ + +C +K +P V+CPD +C TCC PT D +GCCP A CC+DK+HCCP+N+VC++N C +H+ A+ K++ V ++ C + C TCC + +SCCP P AVCC D HCCP+ C+L C+
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPNQQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSS-CPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISC----------VKKAEP-----VMCPDGVSECPVQTTCC--PTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCE 342 HSP 6 Score: 118.627 bits (296), Expect = 3.229e-28 Identity = 88/255 (34.51%), Postives = 130/255 (50.98%), Query Frame = 2
Query: 611 VVTKMKLKIPAISNLSI----ECPDGSSTCKTGATCC-LLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPS--LLIKTFQ---PNPVNR--VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
++T +KL + A++ L + +CP+ C TCC +LS G Y CCPF CC D L CCP +C C + T +KP+ L K+F+ + N+ +ICPD C +C KL D YGCCP + C+D+ HCCP + C+ + +C +AE P++ CP+G S C + TCCP+ + + CCP P AVCC+D HCCP N+ C +N+ C + + P+ P
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPN-QQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSLSWTERKPAQAYLAKSFRMISSSTANKDNIICPD-NSSCPPEFSCLKL--TDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAE-------PVM-----CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFP 239
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 216.468 bits (550), Expect = 1.291e-62 Identity = 142/379 (37.47%), Postives = 184/379 (48.55%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL--------KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLIKTFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKIS-PI----------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI 1282
+G +SCCP AVCC D HCCP +CNL GTC+ V KL K+P + + +V C D + C D ++CC ++G + CCP AVCC D HCCP CNL+AGTC+ V K+ K+P + C D + C TCC G + CCP AVCC D CCP CN+ AGTC+ + K PS I + V P +A + C D TCCK G + CCP A CC D +HCCP CN+ TCD + + LE K S PI ++ C + C TCC N +SCCP+P VCC D HCCP++ +C+L T +CDKN
Sbjct: 307 EGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKVPSRPIKGKKLPETKGSDVPCND-TVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKG-GWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNT 683 HSP 2 Score: 202.216 bits (513), Expect = 2.065e-57 Identity = 135/410 (32.93%), Postives = 180/410 (43.90%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC-----------------------------------------DSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK---------YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI------------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
TTDG + CCP AVCC+DK HCCP+N+ C++N C + LK T S++ +V C D C DG++CC +G ++CCP AVCC D HCCP CNL+AGTC+ V KL K+P ++ C D ++ C TCC S+G + CCP AVCC D + CCP CNL AGTC+ + + K P+ P+ K C D TCCK G + CCP A CC D HCCP CN+ GTC+ + ++ K+ + + C C + TCC + +SCCP P AVCC D HCCP+ C+L +CD
Sbjct: 188 TTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCND-TAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWM-KKVPSRPIKGKKLPETKGSDVPCNDTVACPDETTCCKNVKG-GWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCD 594 HSP 3 Score: 178.333 bits (451), Expect = 8.821e-49 Identity = 125/410 (30.49%), Postives = 178/410 (43.41%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS---------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-----------------------QP-------------------NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI------------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
V + G Y+CCP+ CC D HCCP C + C + + + PA + L+ ++CPD S C SC + D + CCP + + C+D+ HCCP + C+ + +C K + V+ CPDG S C TCC +DG +GCCP AVCC+DK+ CCP+N+VC++ + C S T K + F QP + V V C D C+DG TCCK G + CCP A CC D IHCCP CN+ GTC+ + ++ K+ V ++ C + + C + TCC + + CCP P AVCC D HCCP+ C+L +C+
Sbjct: 38 VLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSSCPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPVM----------------CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPC-DDTVACADGTTCCKNGEG-GWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCE 426 HSP 4 Score: 168.318 bits (425), Expect = 3.083e-45 Identity = 125/364 (34.34%), Postives = 169/364 (46.43%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS------VLKEVVTK-IK-LKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLI----KTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKS 1267
++G + CCP AVCC D HCCP +CNL GTC+ +K+V ++ IK K+P + V C D C D ++CC G + CCP AVCC D QHCCP CN++AGTC+ V K+ K+P + + C + + C+ +TCC G + CCP AVCC D L CCP CNL A TCD + KKPS I K V C D+ + C TCCK + + CCP+ CC D+IHCCP + CN+ TCD ++ + +K +K AN+ V C DH H CP+ T+C K+
Sbjct: 390 SEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKVPSRPIKGKKLPETKGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSC-DSSHFCPASSTCCKDVNAE-WSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVP------MAGPVKASGPPPPEKKKPKENKANE---------PVKCDDH-HSCPDQTTCCFMNKT 734 HSP 5 Score: 140.969 bits (354), Expect = 9.366e-36 Identity = 110/363 (30.30%), Postives = 164/363 (45.18%), Query Frame = 2
Query: 383 VVTKIKLKVPASSILSVV----CPDQSQCDDGSSCC-VMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS---------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGD-SYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI----------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++T +KL V A ++L +V CP+Q C D +CC V++ G + CCP+ CC D HCCP +C + C + + + PA + L+ I CPD SS C +C L+D YGCCP + + C+D+ CCP + C+ + +C +K +P V+CPD +C TCC PT D +GCCP A CC+DK+HCCP+N+VC++N C +H+ A+ K++ V ++ C + C TCC + +SCCP P AVCC D HCCP+ C+L +C+
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPNQQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSS-CPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISC----------VKKAEP-----VMCPDGVSECPVQTTCC--PTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCE 342 HSP 6 Score: 118.627 bits (296), Expect = 3.319e-28 Identity = 88/255 (34.51%), Postives = 130/255 (50.98%), Query Frame = 2
Query: 611 VVTKMKLKIPAISNLSI----ECPDGSSTCKTGATCC-LLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPS--LLIKTFQ---PNPVNR--VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
++T +KL + A++ L + +CP+ C TCC +LS G Y CCPF CC D L CCP +C C + T +KP+ L K+F+ + N+ +ICPD C +C KL D YGCCP + C+D+ HCCP + C+ + +C +AE P++ CP+G S C + TCCP+ + + CCP P AVCC+D HCCP N+ C +N+ C + + P+ P
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPN-QQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSLSWTERKPAQAYLAKSFRMISSSTANKDNIICPD-NSSCPPEFSCLKL--TDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAE-------PVM-----CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFP 239
BLAST of Granulin precursor vs. Ensembl Cavefish
Match: grna (granulin a [Source:ZFIN;Acc:ZDB-GENE-030131-8434]) HSP 1 Score: 215.698 bits (548), Expect = 2.583e-62 Identity = 142/378 (37.57%), Postives = 187/378 (49.47%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS------VLKEVVTK--IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLIKTFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---LEIKIS-PI----------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+G +SCCP AVCC D HCCP +CNL G C+ LK+V ++ + K+P + + +V C D + C D ++CC ++G + CCP AVCC D HCCP CNL+AGTC+ V KL K+P + C D + C TCC G + CCP AVCC D CCP CN+ AGTC+ + K PS I + V P +A + C D TCCK G + CCP A CC D +HCCP CN+ TCD + + LE K S PI ++ C + C TCC N +SCCP+P VCC D HCCP++ +C+L T +CDKN
Sbjct: 307 EGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKGSDVPCND-TVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKG-GWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSCDSSHFCPASSTCCKDVNAEWSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKN 682 HSP 2 Score: 199.904 bits (507), Expect = 1.823e-56 Identity = 134/410 (32.68%), Postives = 182/410 (44.39%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC-----------------------------------------DSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTK--MKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAK---------YQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI------------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
TTDG + CCP AVCC+DK HCCP+N+ C++N C + LK T S++ +V C D C DG++CC +G ++CCP AVCC D HCCP CNL AG C+ LK+V ++ + K+P ++ C D ++ C TCC S+G + CCP AVCC D + CCP CNL AGTC+ + + K P+ P+ K C D TCCK G + CCP A CC D HCCP CN+ GTC+ + ++ K+ + + C C + TCC + +SCCP P AVCC D HCCP+ C+L +CD
Sbjct: 188 TTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCND-TAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWM-KKLPSQPIKGKKLPETKGSDVPCNDTVACPDETTCCKNVKG-GWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPCNATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCD 594 HSP 3 Score: 177.178 bits (448), Expect = 2.716e-48 Identity = 124/410 (30.24%), Postives = 178/410 (43.41%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS---------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF-----------------------QP-------------------NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL------------EIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
V + G Y+CCP+ CC D HCCP C + C + + + PA + L+ ++CPD S C SC + D + CCP + + C+D+ HCCP + C+ + +C K + V+ CPDG S C TCC +DG +GCCP AVCC+DK+ CCP+N+VC++ + C S T K + F QP + V V C D C+DG TCCK G + CCP A CC D +HCCP CN+ G C+ + ++ E K+ V ++ C + + C + TCC + + CCP P AVCC D HCCP+ C+L +C+
Sbjct: 38 VLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSSCPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAEPVM----------------CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPC-DDTVACADGTTCCKNGEG-GWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCEDPSGSVPWLKKVPSRPIREKKLPETKVYNVPCNDTAACPDETTCCKDSEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCE 426 HSP 4 Score: 169.474 bits (428), Expect = 1.161e-45 Identity = 124/364 (34.07%), Postives = 164/364 (45.05%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKL--------KVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKL--------KIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPSLLI----KTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKS 1267
++G + CCP AVCC D HCCP +CNL GTC+ V KL K+P + V C D C D ++CC G + CCP AVCC D QHCCP CN++AGTC+ V K+ K+P + + C + + C+ +TCC G + CCP AVCC D L CCP CNL A TCD + KKPS I K V C D+ + C TCCK + + CCP+ CC D+IHCCP + CN+ TCD ++ + +K +K AN+ V C DH H CP+ T+C K+
Sbjct: 390 SEGEWKCCPLPQAVCCDDFIHCCPHGKKCNLAAGTCEDPSGSVPWMKKLPSQPIKGKKLPETKGSDVPCNDTVACPDETTCCKNVKGGWNCCPLPQAVCCDDFQHCCPHGKKCNVAAGTCEDPSGSVPWIKKVPSRPISGKKLPKVKGAGVPC-NATFACEDESTCCKNDKGGWSCCPLPEAVCCEDFLHCCPHGKKCNLAAMTCDDPSGSIPWLEKKPSRPIGGQNKEKGKAAAANVSC-DSSHFCPASSTCCKDVNAE-WSCCPFPQGVCCEDRIHCCPHDATCNLRTLTCDKNTTSVP------MAGPVKASGPPPPEKKKPKENKANE---------PVKCDDH-HSCPDQTTCCFMNKT 734 HSP 5 Score: 140.198 bits (352), Expect = 1.902e-35 Identity = 110/363 (30.30%), Postives = 163/363 (44.90%), Query Frame = 2
Query: 383 VVTKIKLKVPASSILSVV----CPDQSQCDDGSSCC-VMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS---------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGD-SYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----------------------DHQAEALEIKISPI----------------------LVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
++T +KL V A ++L +V CP+Q C D +CC V++ G + CCP+ CC D HCCP +C + C + + + PA + L+ I CPD SS C +C L+D YGCCP + + C+D+ CCP + C+ + +C +K +P V+CPD +C TCC PT D +GCCP A CC+DK+HCCP+N+VC++N C +H+ A+ K++ V ++ C + C TCC + +SCCP P AVCC D HCCP+ C+L C+
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPNQQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSL--SWTERKPAQAYLAKSFRMISSSTANKDNIICPDNSS-CPPEFSCLKLTD-KYGCCPVTQGIVCADRNHCCPNDHECSADSISC----------VKKAEP-----VMCPDGVSECPVQTTCC--PTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFPARLRADWENHRPVAVTPKLTNKQPKTSTALKSTTTNKTRNATVSTVYNVPCDDTVACADGTTCCKNGEGGWSCCPLPQAVCCDDFLHCCPHGKKCNLIAGKCE 342 HSP 6 Score: 118.627 bits (296), Expect = 3.259e-28 Identity = 88/255 (34.51%), Postives = 130/255 (50.98%), Query Frame = 2
Query: 611 VVTKMKLKIPAISNLSI----ECPDGSSTCKTGATCC-LLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT-------KKPS--LLIKTFQ---PNPVNR--VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
++T +KL + A++ L + +CP+ C TCC +LS G Y CCPF CC D L CCP +C C + T +KP+ L K+F+ + N+ +ICPD C +C KL D YGCCP + C+D+ HCCP + C+ + +C +AE P++ CP+G S C + TCCP+ + + CCP P AVCC+D HCCP N+ C +N+ C + + P+ P
Sbjct: 2 LLTMLKLMV-ALALLGLVTGTQCPN-QQLCGDKHTCCQVLSSGEYNCCPFPQGECCEDHLHCCPAGMLCQTAESKCQNSTHSLSWTERKPAQAYLAKSFRMISSSTANKDNIICPD-NSSCPPEFSCLKL--TDKYGCCPVTQGIVCADRNHCCPNDHECSADSISCVKKAE-------PVM-----CPDGVSECPVQTTCCPTTDGGWGCCPMPRAVCCADKVHCCPENSVCHVNSSKCVSSTNKELPMWAKFP 239
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000009302.1 (pep scaffold:Pmarinus_7.0:GL483232:1183:11161:1 gene:ENSPMAG00000008413.1 transcript:ENSPMAT00000009302.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 258.84 bits (660), Expect = 5.691e-79 Identity = 157/364 (43.13%), Postives = 193/364 (53.02%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK---LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKI-SPILV-----ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
T G ++CCP AVCC D +HCCPQ T CNL TCD + K PA + V+C DQS C DG++CC M G++ CCP AVCC D QHCCP+ T CNL CD + K L++ N+ I D +C G TCC L G +GCCP AVCC D CCPQ T CNLPA TCD TK P+L + N VIC D ++ C DG TCCK+P+GD +GCCP A CC D HCCPQ T CN+ TCD A +L +P L +KC + C + TCC + + CCP AVCC DH HCCP T+C++ SCD+ L
Sbjct: 307 TPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQ--GTLSLPWSTKTPALRVAGEPNVVICDDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTLSLPWSTKTPALRVAGEPNVVIC--DDQFSCPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVA----GEPNVVIC-DDQFSCPDGDTCCKMPSGD-WGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRVAGEPNDVKCDDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQGQL 660 HSP 2 Score: 258.455 bits (659), Expect = 7.698e-79 Identity = 158/370 (42.70%), Postives = 195/370 (52.70%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK---LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---------QAEALEIKISPILV---ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
T G ++CCP AVCC D +HCCPQ T CNL TCD + K PA + V+C DQS C DG++CC G++ACCP AVCC D +HCCPQ T CNL A TCD + K L++ N+ I D S+C G TCC + G +GCCP AVCC D CCP+ T CNLP CD TK P+L + N VIC D ++ C DG TCCKLP+GD +GCCP A CC D HCCPQ T CN+ TCD + AL + P +V + CP+G TCC + + CCP AVCC DH HCCP T+C+L ++CDK L
Sbjct: 229 TPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQ--GTLSLPWSTKTPALRVAGEPNVVICDDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC--DDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTLSLPWSTKTPALRVA----GEPNVVIC-DDQFSCPDGNTCCKLPSGD-WGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVICDDQFSCPDGD------TCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGAL 582 HSP 3 Score: 241.121 bits (614), Expect = 2.427e-72 Identity = 151/371 (40.70%), Postives = 191/371 (51.48%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK---LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---------QAEALEIKISPILV---ESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
+ G + CCP AVCC DK+HCCP+ T CN+ TC+ + K PA + V C D C DG++CC G++ACCP AVCC D +HCCPQ T CNL A TCD + K L++ N+ I D S+C G TCC G + CCP AVCC D CCPQ T CNLPA TCD TK P+L + N VIC D + C DG TCCK+P+GD +GCCP A CC D HCCP+ T CN+ CD + AL + P +V + CP+G+ TCC + + CCP AVCC DH HCCP T+C+L ++CD+ L
Sbjct: 150 ILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSL--PWSTKTPALRMTGEPNDVQCDDLVGCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC--DDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVA----GEPNVVIC-DDQSSCPDGNTCCKMPSGD-WGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTLSLPWSTKTPALRVAGEPNVVICDDQFSCPDGN------TCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTL 504 HSP 4 Score: 234.187 bits (596), Expect = 8.331e-70 Identity = 148/368 (40.22%), Postives = 185/368 (50.27%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK---LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL--------EIKISPILVES-------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
G + CCP AVCC D QHCCP+ T CNL CD + K PA + V+C DQ C DG++CC + G++ CCP AVCC D +HCCPQ T CNL A TCD + K L++ N+ I D +C G TCC + G +GCCP AVCC D CCPQ T CNLPA TCD TK P+L + +PN V D + C D TCCK TGD +GCCP A CC D HCCP+ T CN+ G+CD +L +++ +L S ++C + C TCC + CCP P A CCSD HCCP+ +C L SC K
Sbjct: 388 GDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDK--GTLSLPWSTKTPALRVAGEPNVVICDDQFSCPDGNTCCKLPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVVIC--DDQFSCPDGDTCCKMPSGDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRV-AGEPNDVKC----DDQVSCPDQTTCCKTGTGD-WGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQGQLSLPWSSKTPARTRLTALLPASAAARGGSVRCDSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASCIKK 745 HSP 5 Score: 233.417 bits (594), Expect = 1.498e-69 Identity = 149/361 (41.27%), Postives = 187/361 (51.80%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS-VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK---LKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---------QAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
G Y CC AVCCSD HCCP+NT C+L G C V ++ K P+ S V C + C D ++CC++ G + CCP AVCC DK+HCCP+ T+CN+++ TC+ + K L++ N ++C D C G TCC G + CCP AVCC D CCPQ T CNLPA TCD TK P+L + N VIC D + C DG TCCK P GD + CCP A CC D HCCPQ T CN+ TCD + AL + P +V C + S C TCC + + CCP P AVCC DH HCCP T+C+L + CDK L
Sbjct: 79 GQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKC--VHGKLSIPWVSKYPSKQAASDVQCDAFTSCSDQNTCCILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSLPWSTKTPALRMTGEPN-DVQC-DDLVGCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVA----GEPNVVIC-DDQSSCPDGNTCCKTPGGD-WACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVV---ICDDQSSCPDGNTCCKMPSGDWGCCPLPKAVCCDDHQHCCPEGTTCNLPVERCDKGTL 426 HSP 6 Score: 215.698 bits (548), Expect = 6.004e-63 Identity = 143/365 (39.18%), Postives = 184/365 (50.41%), Query Frame = 2
Query: 293 AVCCSDKQHCCPQNTECNLNKGTCDSVLKEV--------VTKIKLKVPASSILSVV---CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNL-------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS-------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH---------QAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL 1285
AVCCSD HCCP T C+L G C V T + + L+V CPD + C D ++CC +A G + CC AVCCSD HCCP+NT+C+L G C KL IP +S ++C D ++C TCC+L G +GCCP AVCC DK CCP+ T+CN+ + TC+ TK P+L + T +PN V D C DG TCCK P GD + CCP A CC D HCCPQ T CN+ TCD + AL + P +V C + S C TCC + ++CCP AVCC DH HCCP T+C+L ++CD+ L
Sbjct: 2 AVCCSDGLHCCPSGTVCDLAHGMCQKGKISVPWLEKTPTFTPRTWRWRLARSLAVRPPQCPDGTSCGDDATCCPLASGQYGCCSLEDAVCCSDYLHCCPKNTMCDLKLGKC--------VHGKLSIPWVSKYPSKQAASDVQC-DAFTSCSDQNTCCILPSGQWGCCPLPKAVCCDDKEHCCPEGTICNVTSQTCNHAALSLPWSTKTPALRM-TGEPNDVQC----DDLVGCPDGNTCCKTPGGD-WACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTLSLPWSTKTPALRVAGEPNVV---ICDDQSSCPDGNTCCKTPGGDWACCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDQGTL 348 HSP 7 Score: 140.584 bits (353), Expect = 2.998e-36 Identity = 88/218 (40.37%), Postives = 110/218 (50.46%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIK---LKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCD--------SKLKEVVTKMKLKIP---AISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPN 874
G + CCP AVCC D +HCCPQ T CNL TCD + K L+V A V C DQ C D ++CC G++ CCP AVCC D +HCCP+ T CN+ AG+CD S T++ +P A S+ C D + C G TCC L G +GCCP A CCSD + CCP C+L +C KKP L T+ P
Sbjct: 544 GDWGCCPLVKAVCCDDHEHCCPQGTTCNLPAQTCDKGALSLPWSTKTPALRV-AGEPNDVKCDDQVSCPDQTTCCKTGTGDWGCCPLVKAVCCDDHEHCCPEGTTCNVPAGSCDQGQLSLPWSSKTPARTRLTALLPASAAARGGSVRC-DSAYACPDGTTCCQLPQGQWGCCPLPLATCCSDFVHCCPSGYTCDLAQASC---IKKPHFL--TWAPK 754
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001039.1 (pep scaffold:Pmarinus_7.0:GL484091:11570:24665:-1 gene:ENSPMAG00000000936.1 transcript:ENSPMAT00000001039.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 152.91 bits (385), Expect = 5.012e-41 Identity = 115/343 (33.53%), Postives = 150/343 (43.73%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTK-IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV--NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALE-IKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
G + CC A CCSD HCCP T C + + + L + K L P L V C C +CC G + CC A CCSD HCCP T C + + L + K + + + C G C+ TCC G +GCC A CCSD + CCP +L +S P L + PV N V C +Y C QTCC+ G +GCC ATCCSD IHCCP T C + C +EAL + S L ++ + C + TCC S + CC A CCSD HCCP+ T C + + C ++
Sbjct: 239 GMWGCCNGRRATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNL-VHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNVVHC-GGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQY-CRRWQTCCRSRRG-MWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGSSTLSHALPYDSRVYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRS 577 HSP 2 Score: 149.058 bits (375), Expect = 1.059e-39 Identity = 121/352 (34.38%), Postives = 156/352 (44.32%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTK-IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTK-MKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV--NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKIS-PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+ G + CC A CCSD HCCP T C + + + L + K L P + V C C +CC G + CC A CCSD HCCP T C + + L + K L P NL + C G C+ TCC G +GCC A CCSD + CCP T C L +S P L + PV N V C +Y C QTCC+ G +GCC ATCCSD IHCCP ++ KG C + A + E +S P+ + C G C + TCC S + CC A CCSD HCCP+ T C + + C ++
Sbjct: 160 SRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNV-VHCGGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNL-VHC-GGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRC-LVGQCVNSAALIPWLKKEAALSGPVGDNVVHCGGGQY-CRRWQTCCRSRRG-MWGCCNGRRATCCSDGIHCCPSGASGDL-KGQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRS 504 HSP 3 Score: 149.058 bits (375), Expect = 1.146e-39 Identity = 117/353 (33.14%), Postives = 151/353 (42.78%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV--NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKIS-PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
G + CC A CCSD HCCP T C + C + + L +VV C C +CC G + CC A CCSD HCCP T C + + L + K + + + C G C+ TCC G +GCC A CCSD + CCP T C L +S P L + PV N V C +Y C QTCC+ G +GCC ATCCSD IHCCP T C + G C + A + E +S P+ + C G C + TCC S + CC A CCSD HCCP+ S L + + L+ +
Sbjct: 86 GMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSVLDYLSREQQDGYNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNVVHC-GGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRC-LVGQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQY-CRRWQTCCRSRCG-MWGCCNGRKATCCSDGIHCCPSMTRCLV--GQCVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQCVNSAALIPW 432 HSP 4 Score: 147.132 bits (370), Expect = 5.583e-39 Identity = 117/341 (34.31%), Postives = 149/341 (43.70%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNL-NKGTC---DSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPV--NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKIS-PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
G + CC A CCSD HCCP T C + +G C +++ + + L P L V C C +CC G + CC A CCSD HCCP T C + C + + L ++ G C+ TCC G +GCC A CCSD + CCP T C L +S P L + PV N V C +Y C QTCC+ G +GCC ATCCSD IHCCP T C + G C + A + E +S P+ + C G C + TCC S + CC A CCSD HCCP+ T C
Sbjct: 7 GMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAALIPWLKKEAALSGPVGDNL-VHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSVLDYLSREQQDGYNVVHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRC-LVGQCVNSAALIPWLKKEAALSGPVGDNVVHCGGGQY-CRRWQTCCRSRCG-MWGCCNGRRATCCSDGIHCCPSMTRCLV--GQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRC 341 HSP 5 Score: 128.642 bits (322), Expect = 1.539e-32 Identity = 99/271 (36.53%), Postives = 127/271 (46.86%), Query Frame = 2
Query: 473 CCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNL-SAGTC---DSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLL--IKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKIS-PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
CC G + CC A CCSD HCCP T C + G C + + + + L P NL + C G C+ TCC G +GCC A CCSD + CCP T C +P C ++ S+L + Q + N V C +Y C QTCC+ G +GCC ATCCSD IHCCP T C + G C + A + E +S P+ + C G C + TCC S + CC A CCSD HCCP+ T C
Sbjct: 1 CCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAALIPWLKKEAALSGPVGDNL-VHC-GGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSVLDYLSREQQDGYNVVHCGGGQY-CRRWQTCCRSRRG-MWGCCNGRRATCCSDGIHCCPSMTRCLV--GQCVNSAALIPWLKKEAALSGPVGDNVVHCGGGQYCRRWQTCCRSRCGMWGCCNGRRATCCSDGIHCCPSMTRC 265 HSP 6 Score: 124.405 bits (311), Expect = 5.065e-31 Identity = 92/281 (32.74%), Postives = 122/281 (43.42%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTK-IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK--LKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPI 1096
G + CC A CCSD HCCP T C + + + L + K L P + V C C +CC G + CC A CCSD HCCP +L +S + + + L P NL + C G C+ TCC G +GCC A CCSD + CCP T C +P C ++ L + ++ + D++ C QTCC+ G +GCC ATCCSD IHCCP T C + C +EAL I
Sbjct: 315 GMWGCCNGRKATCCSDGIHCCPSMTRCLVGQCVNSAALIPWLKKEAALSGPVGDNV-VHCGGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSGASGDLKGQCVNSAALIPWLKKEAALSGPVGDNL-VHC-GGGQYCRRWQTCCRSRRGMWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLC---SRSSEALSGVAGSSTLSHALPYDSRVYCRRWQTCCRSRCG-MWGCCNGRRATCCSDGIHCCPSMTRCLVPYRLCSRSSEALSGVAGRI 588 HSP 7 Score: 67.3958 bits (163), Expect = 2.834e-12 Identity = 43/114 (37.72%), Postives = 58/114 (50.88%), Query Frame = 2
Query: 959 YGCCPYSAATCCSDKIHCCPQNTVCNI-NKGTCDHQAEAL-----EIKIS-PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
+GCC ATCCSD IHCCP T C + +G C + A + E +S P+ + C G C + TCC S + CC A CCSD HCCP+ T C + + C ++
Sbjct: 9 WGCCNGRKATCCSDGIHCCPSMTRCLVPYRGQCVNSAALIPWLKKEAALSGPVGDNLVHCGGGQYCRRWQTCCRSRCGMWGCCNGRKATCCSDGIHCCPSMTRCLVPYRLCSRS 122
BLAST of Granulin precursor vs. Ensembl Nematostella
Match: EDO27824 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T870]) HSP 1 Score: 123.25 bits (308), Expect = 9.829e-33 Identity = 83/229 (36.24%), Postives = 111/229 (48.47%), Query Frame = 2
Query: 413 ASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCD-SKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNP--------VNRVI------CPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKG 1048
AS + C D S C D +CC ++ G++ CCP +AVCCSD +HCCP C++S+ TCD +K + + I+C DGS C TG TCCL+ YGCCP +AVCC+D CCP CN +G C + K + P P ++ I A C TCC + CCP A+CCSD +HCCPQ +C+ KG
Sbjct: 1 ASGANYITCNDGSHCYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQRAGVGEIKCKDGSH-CNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQ-NCCPLPNASCCSDGLHCCPQGFICDDQKG 227 HSP 2 Score: 119.783 bits (299), Expect = 2.286e-31 Identity = 74/201 (36.82%), Postives = 96/201 (47.76%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEV-VTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK----------LKIPAISNLSIE-CPDGSS----TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAG 817
GSY CCP +AVCCSD +HCCP C+++ TCD + V + + + C D S C+ G +CC++ + CCP SAVCC+D QHCCP CN +G C + K K P I + +I P G C+ TCC S CCP NA CCSD L CCPQ +C+ G
Sbjct: 27 GSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQRAGVGEIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICDDQKG 227 HSP 3 Score: 115.931 bits (289), Expect = 5.407e-30 Identity = 91/230 (39.57%), Postives = 115/230 (50.00%), Query Frame = 2
Query: 659 IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCD-SKTKKPSL-LIKTFQPNPVNRVICPDAKYQCSDGQTCCKL-PTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-------------EIKISPILVE-SIK-CPNGS-----LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
I C DGS C TCC LS GSYGCCP NAVCCSD CCP C++ + TCD +K P + T Q V + C D + C+ G+TCC + PT YGCCP +A CC+D HCCP CN G C L + +P++++ +I+ P G C TCC S++ +CCP PNA CCSD HCCP C + K C K
Sbjct: 7 ITCNDGSH-CYDYETCCELSTGSYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQRAGVGEIKCKDGSH-CNTGETCCLVGPT--KYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHVLPMFQKHPSVHPRPAVAKTPVIIDHTIRFVPRGGRGAGCACEPYHTCCGSSSAQQNCCPLPNASCCSDGLHCCPQGFICD-DQKGCVKQ 231 HSP 4 Score: 97.8265 bits (242), Expect = 1.943e-23 Identity = 60/151 (39.74%), Postives = 79/151 (52.32%), Query Frame = 2
Query: 878 VNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA-----LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
N + C D + C D +TCC+L TG SYGCCP A CCSD HCCP C+I+ TCD ++ + V IKC +GS C TCC Y CCP P+AVCC+D HCCP+ +C+ + C + + P+ HP
Sbjct: 4 ANYITCNDGSH-CYDYETCCELSTG-SYGCCPLPNAVCCSDGKHCCPCGYHCDISSSTCDMAKDSTPMVSYHATVQRAGVGEIKCKDGSHCNTGETCCLVGPTKYGCCPLPSAVCCADMQHCCPSGYTCNDKSGHCQRGRHV-LPMFQKHP 151
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grnb (granulin precursor [Source:NCBI gene;Acc:101166910]) HSP 1 Score: 253.447 bits (646), Expect = 3.704e-76 Identity = 152/370 (41.08%), Postives = 204/370 (55.14%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSI---------LSVVCP-DQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK---TKKPSLLIKTFQP---NPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDH-QAEALEIKIS-----PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPI 1300
DGS+ CCP+ AVCC D++HCCP+ T C+L C S + ++ KVPA + SVVCP +S C + +CC++ G+F CCPY A CCSD HCCP NT C+L G C S V ++ + S + CPD S C G TCC L++G++GCCP NAVCCSD + CCP+ T C+L TC S T + ++ + P +PV + P +A C+DG TCC+ G +GCCP A CC D +HCCP TVCN+ TCD AL +S P E+ +C + C + TCC + ++CCP P AVCC DH HCCPN T C+L ++CD L P+
Sbjct: 69 DGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQ-KVPAQRVGTLPVFAAAASVVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSGEARV---EPIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPCNASVACADGSTCCRASRGQ-WGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRCDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPL 433 HSP 2 Score: 232.261 bits (591), Expect = 2.826e-68 Identity = 143/373 (38.34%), Postives = 190/373 (50.94%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPA-------ISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKK---PSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQA----EALEIKISPIL---VESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
+ T G + CCPY A CCSD HCCP NT C+L +G C S V ++ ++S V+CPDQ S C DG++CC + +G F CCP +AVCCSD HCCP+ T C+L+ TC S + + + + L++ C + S C G+TCC S G +GCCP AVCC D L CCP TVCNL A TCD + P L P C D C TCC+ G + CCP A CC D IHCCP T+CN+ TCD + + P L +E KC ++C + TCC +++ ++CCP P AVCCSD HCCP C+ ++C++ P P
Sbjct: 151 LLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSGEARVE---PIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPC-NASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRC-DESASCPGEATCCRTRAG-GWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGMEEEKCGKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLP 517 HSP 3 Score: 224.557 bits (571), Expect = 1.545e-65 Identity = 129/330 (39.09%), Postives = 184/330 (55.76%), Query Frame = 2
Query: 407 VPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPA---------ISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----DHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF-PIINFHP*FNYY 1330
+P + + +V+CPDQ S+C D ++CC + DG++ CCP+ AVCC D++HCCP+ T C+L C S + ++ K+PA + S+ CP G S+C TCCLL+ G +GCCP+ A CCSD L CCP NT C+L G C S + I+ +P + V+CPD + C DG TCC+L G ++GCCP A CCSD +HCCP+ T C++ TC AEA+ + +S P+ ++ C C TCC ++ + CCP P AVCC DH HCCP+ T C+L +CD P+++ P F Y
Sbjct: 41 LPHTRVGAVICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQ-KVPAQRVGTLPVFAAAASVVCPGGKSSCPNSFTCCLLTSGDFGCCPYPQATCCSDHLHCCPGNTTCDLERGVCKSGEAR-VEPIRAVSASPED-VLCPDQRSMCPDGTTCCQLNNG-TFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPCNASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYR 366 HSP 4 Score: 208.764 bits (530), Expect = 9.767e-60 Identity = 130/372 (34.95%), Postives = 172/372 (46.24%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSV--VCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCD--SKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDS--------KTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
+ G + CCP AVCC D HCCP T CNL TCD +T + P S C + + C ++CC G +ACCP AVCC D HCCP T+CNL A TCD S L + + + + + E + C TCC S G + CCP AVCCSD CCP+ CN TC+ + K P+L ++ Q PV DA+ C TCC + +GCCP A CC D HCCP+ C ++ +C + + P V +KC N S C TCCP + CCP AVCC+DH HCCP + +C++ T +C+K P+ P
Sbjct: 309 ASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRCDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGMEEEKCGKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSGQTGPVENKC--DAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVVPWFTKVGALTEPSGVTDVKCDNKSSCASGTTCCPLQTGGWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQGLPLSRVVP 678 HSP 5 Score: 201.445 bits (511), Expect = 4.547e-57 Identity = 145/412 (35.19%), Postives = 189/412 (45.87%), Query Frame = 2
Query: 131 HDVYRFSREFIKPITETNKIPVKVVNXXXXXXXXXXXXXXVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPAS-------SILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP-DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSK----------TKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD----------HQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSAND-SYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
V + ++PI + P V+ S+C DG++CC +G++ CCP +AVCCSD HCCP+ T C+L TC S + + V + L+V C C DGS+CC + G + CCP AVCC D HCCP TVCNL A TCD +T + P S D S++C ATCC G + CCP AVCC D + CCP T+CNL A TCD TK+P L + +CP TCCK +G + CCP A CCSD HCCP+ CN TC+ + AL+ + KC + C + TCC + CCP P AVCC D +HCCP SC + SC +
Sbjct: 185 RGVCKSGEARVEPIRAVSASPEDVLCPDQRSMCPDGTTCCQLNNGTFGCCPLPNAVCCSDHMHCCPEGTTCDLAHSTCVSAAGGTAEAVMVPVSGTPPKTHPVRGLAVPCNASVACADGSTCCRASRGQWGCCPLPEAVCCEDHLHCCPHGTVCNLVASTCDDPSGSALTPLLSNTPGFPYRSENGRCDESASCPGEATCCRTRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRLTAGMEEEKCGKDAVCPGRT-------TCCKNSSGQ-WACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSLPWRQKLPALQQRSGQTGPVENKCDAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRG 588 HSP 6 Score: 186.808 bits (473), Expect = 1.094e-51 Identity = 128/370 (34.59%), Postives = 173/370 (46.76%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV------LKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP------DGSSTCKTGATCCLLSDG-SYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF---QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAE-----ALEIKISPILVESIKCPN-GSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKF 1294
T G ++CCP AVCC D HCCP T CNL TCD L + + +L + + C + C ++CC + G +ACCP AVCCSD HCCP+ CN TC+ + + K+PA+ S + D ++C TCC + +GCCP AVCC D CCP+ C +C + K +P+ V V C D K C+ G TCC L TG +GCCP A CC+D HCCPQ+ CN+ GTC+ +A + + P + + C G C ++ TCC + ++ CCP P AVCC D HCCP SC L C L +
Sbjct: 387 TRAGGWACCPLPQAVCCDDHIHCCPNATICNLEAETCDDPSGLSPPLPWLTKRPRL---TAGMEEEKCGKDAVCPGRTTCCKNSSGQWACCPLPQAVCCSDLDHCCPKGYKCNAFDRTCERPGGPSL-PWRQKLPALQQRSGQTGPVENKCDAQTSCPKDTTCCFMEQTLKWGCCPLPQAVCCGDGNHCCPRGHSCQPHRSSCSRGSHVVPWFTKVGALTEPSGVTDVKC-DNKSSCASGTTCCPLQTG-GWGCCPLVKAVCCADHEHCCPQSYTCNMQTGTCEKKAGQGLPLSRVVPAQPQDTQDVPCDGTGEFKCPKQNTCCRISATAWGCCPSPGAVCCMDSKHCCPAGYSCDLKAGGCTLGPRLSW 750 HSP 7 Score: 58.5362 bits (140), Expect = 7.764e-9 Identity = 34/102 (33.33%), Postives = 46/102 (45.10%), Query Frame = 2
Query: 1013 CPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP VC + + V ++ CP+ S C + TCC + S+ CCP+P AVCC D HCCP T+C L C L FP++ P
Sbjct: 17 CPDGAVCADGHKCFLNSHGGFTCPLPHTRVGAVICPDQESECPDETTCCQLPDGSWGCCPFPKAVCCEDQRHCCPEGTTCDLVHSKCASPSLQAFPMLQKVP 118
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 237.654 bits (605), Expect = 5.603e-69 Identity = 144/365 (39.45%), Postives = 195/365 (53.42%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILL 1288
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ SN+S++ D GS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + K+S + +E + C + C + TCC + + ++ CCP P AVCC DH HCCP T+C L+T SC K L
Sbjct: 390 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVS-VKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGL 748 HSP 2 Score: 230.72 bits (587), Expect = 1.432e-66 Identity = 139/375 (37.07%), Postives = 194/375 (51.73%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP----DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ SN+S++ DGS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 311 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 680 HSP 3 Score: 209.534 bits (532), Expect = 4.830e-59 Identity = 136/416 (32.69%), Postives = 195/416 (46.88%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC----DSVL--------------------KEV------------VTKIKLKVPASSILS------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
+G ++CCP AVCC DK HCCP+ T C++ + C D L KEV ++ P + L + C + + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ S +S++ D GS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 189 EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 601 HSP 4 Score: 202.986 bits (515), Expect = 8.534e-57 Identity = 140/410 (34.15%), Postives = 188/410 (45.85%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNL-------PAGTCDSKTKKP---------SLLIKTFQP------NPVNR----------------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIK-------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP*F 1321
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ S +S++ D GS++C TCC DG++GCCP AVCC D CCP T C+L +G K P S L+KT Q P +R +I D+ QC TCC + +GCCP A CC+D HCCP + C +C + +SI+ C C + +CC + CCP PNAVCC D HCCP +C L +KSC K + L+ + P F
Sbjct: 548 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVF 953 HSP 5 Score: 174.866 bits (442), Expect = 4.052e-47 Identity = 135/426 (31.69%), Postives = 193/426 (45.31%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV-VTKMKLKIPAISNLS----------------------------------------IECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPD-------AKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIK---ISPILVESIKCPNGSL------------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP*FN 1324
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC DG + CCP AVCC D +HCCP T C+LS +C LK + M K+PA + S I C D + C TCC + +GCCP AVCC+D CCP + C D+ K ++I + P I D A +QC + +CC+L TG+ +GCCP A CC DK HCCP+ C++ +C H+ L+++ ++P+ + + +G + C TCC +++ ++ CC P AVCC D HCCP+ C+ SC KN L + +N+H F
Sbjct: 627 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSC---LKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISC-DSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEE----DTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGE-WGCCPLPNAVCCGDKEHCCPEGYTCDLASKSC-HKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCT-KGGSCIKNTGLHW--LNWHTFFT 1037 HSP 6 Score: 173.326 bits (438), Expect = 1.274e-46 Identity = 122/421 (28.98%), Postives = 186/421 (44.18%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV------LKEVVTKIKLKVPASSILSV--------VCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF----------------------------------QPNPV-----NRVICPDAKYQ---------CSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
Y CCP+ A CC D HCCP++ C++ +C + ++ V +++ + ++ +CPDQS+C SC + A + CCP + V C D +HCCP+ C+L + +C V K +K ++ C DG S C TCC +G + CCP AVCC DK+ CCP+ T C++ C S + K + F NPV + +A + C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + K+S + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 40 YDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSC-LKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC-------VKKESIK-------TVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVS-VKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 443 HSP 7 Score: 169.859 bits (429), Expect = 2.323e-45 Identity = 127/417 (30.46%), Postives = 183/417 (43.88%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQ-------HCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPA--------------ISNLSIE------------------CPDGSS----------------TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
Y CCP + V C D +HCCP+ +C+L+ +C V SI +V+C D S+C D ++CC +G +ACCP K HCCP+ T C++ C S + + M K PA +S +++E +GSS +C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 129 YGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC--------------VKKESIKTVLCKDGVSECPDETTCCENQEGKWACCPMP-------KAVCCDDKIHCCPEETTCDVEQMKCLSSSDKAL-PMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 522 HSP 8 Score: 137.502 bits (345), Expect = 1.843e-34 Identity = 99/369 (26.83%), Postives = 159/369 (43.09%), Query Frame = 2
Query: 407 VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNL-SIECPDGSSTCKTGATC-----CLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----------------------DHQAEALEIKI-------------------SPILVES-----------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
V + + + +CP+ +C+ G +CC ++ CCP+ A CC D HCCP++ +C+++ +C + ++ V +++ + + S D + C + C CL + YGCCP + V C D CCP+ C+L + +C KK S + V+C D +C D TCC+ G + CCP A CC DKIHCCP+ T C++ + C ++Q E E+ P +E I C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 10 VSLALVAADMCPNGGKCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC---VKKES----------IKTVLCKDGVSECPDETTCCENQEG-KWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 364 HSP 9 Score: 106.301 bits (264), Expect = 5.147e-24 Identity = 71/234 (30.34%), Postives = 109/234 (46.58%), Query Frame = 2
Query: 665 CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT----------------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP+G C+ G TCC Y CCPF A CC D + CCP++ +C++ +C + T K +I+++ + ICPD + +C +C K T YGCCP + C D HCCP+ C+++ +C + ++++ C +G S C + TCC + ++CCP P AVCC D HCCP T+C + C + P+ P
Sbjct: 20 CPNGGK-CEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDN-ICPD-QSRCPAEFSCLKALT--RYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC----------VKKESIKTVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFP 238 HSP 10 Score: 104.76 bits (260), Expect = 1.620e-23 Identity = 75/229 (32.75%), Postives = 100/229 (43.67%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSS----------------TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI 892
+ CCP AVCC+D +HCCP + +C +C V EVV K+PA+ + V C QC + SCC + G + CCP +AVCC DK+HCCP+ TCD K + L++ + + PD S +C TCC S ++GCC AVCC D CCP C G+C T L TF N +I
Sbjct: 826 WGCCPLPKAVCCADGEHCCPADYKCKEEDTSC--VKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGY-------TCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCTK-GGSCIKNTGLHWLNWHTFFTNKRKAII 1044
BLAST of Granulin precursor vs. Ensembl Medaka
Match: grna (granulins [Source:NCBI gene;Acc:101162601]) HSP 1 Score: 234.958 bits (598), Expect = 6.707e-68 Identity = 143/365 (39.18%), Postives = 194/365 (53.15%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILL 1288
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ S +S++ D GS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + K+S + +E + C + C + TCC + + ++ CCP P AVCC DH HCCP T+C L+T SC K L
Sbjct: 469 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVS-VKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGL 827 HSP 2 Score: 231.876 bits (590), Expect = 8.185e-67 Identity = 141/376 (37.50%), Postives = 197/376 (52.39%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECP----DGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ SN+S++ DGS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + K+S + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 311 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVS-VKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 680 HSP 3 Score: 209.149 bits (531), Expect = 8.568e-59 Identity = 136/416 (32.69%), Postives = 195/416 (46.88%), Query Frame = 2
Query: 260 DGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC----DSVL--------------------KEV------------VTKIKLKVPASSILS------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
+G ++CCP AVCC DK HCCP+ T C++ + C D L KEV ++ P + L + C + + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ S +S++ D GS++C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 189 EGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 601 HSP 4 Score: 202.601 bits (514), Expect = 1.506e-56 Identity = 140/410 (34.15%), Postives = 188/410 (45.85%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPD----GSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNL-------PAGTCDSKTKKP---------SLLIKTFQP------NPVNR----------------VICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIK-------CPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP*F 1321
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC +G + CCP+ AVCC D HCCP+ CNL+A TC+ V M K+P+ S +S++ D GS++C TCC DG++GCCP AVCC D CCP T C+L +G K P S L+KT Q P +R +I D+ QC TCC + +GCCP A CC+D HCCP + C +C + +SI+ C C + +CC + CCP PNAVCC D HCCP +C L +KSC K + L+ + P F
Sbjct: 627 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSCLKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISCDSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEEDTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGYTCDLASKSCHKLLTLQLETVPLTPVF 1032 HSP 5 Score: 174.481 bits (441), Expect = 6.309e-47 Identity = 135/426 (31.69%), Postives = 193/426 (45.31%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVV-----CPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEV-VTKMKLKIPAISNLS----------------------------------------IECPDGSSTCKTGATCCLLSD-GSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPD-------AKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIK---ISPILVESIKCPNGSL------------CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP*FN 1324
T +G + CCP+ AVCC D HCCP+ CNL TC+ V + KVP+ S +SV C + C D ++CC DG + CCP AVCC D +HCCP T C+LS +C LK + M K+PA + S I C D + C TCC + +GCCP AVCC+D CCP + C D+ K ++I + P I D A +QC + +CC+L TG+ +GCCP A CC DK HCCP+ C++ +C H+ L+++ ++P+ + + +G + C TCC +++ ++ CC P AVCC D HCCP+ C+ SC KN L + +N+H F
Sbjct: 706 TQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSV--PMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTKDGAWGCCPLPEAVCCDDHEHCCPTGTTCDLSTLSC---LKASGLVPMSKKLPASTRTSSATSQLMKTGQETEEGENKPDDRDGQEDFDEEDKTDEEGIISC-DSHTQCPQHTTCCFMKQVQKWGCCPLPKAVCCADGEHCCPADYKCKEE----DTSCVKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGE-WGCCPLPNAVCCGDKEHCCPEGYTCDLASKSC-HKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCT-KGGSCIKNTGLHW--LNWHTFFT 1116 HSP 6 Score: 172.94 bits (437), Expect = 2.195e-46 Identity = 122/421 (28.98%), Postives = 186/421 (44.18%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSV------LKEVVTKIKLKVPASSILSV--------VCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTF----------------------------------QPNPV-----NRVICPDAKYQ---------CSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEI--------KISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
Y CCP+ A CC D HCCP++ C++ +C + ++ V +++ + ++ +CPDQS+C SC + A + CCP + V C D +HCCP+ C+L + +C V K +K ++ C DG S C TCC +G + CCP AVCC DK+ CCP+ T C++ C S + K + F NPV + +A + C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + K+S + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 40 YDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSC-LKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC-------VKKESIK-------TVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVS-VKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 443 HSP 7 Score: 169.088 bits (427), Expect = 3.688e-45 Identity = 127/417 (30.46%), Postives = 183/417 (43.88%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQ-SQCDDGSSCCVMADGNFACCPYSSAVCCSDKQ-------HCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPA--------------ISNLSIE------------------CPDGSS----------------TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIK--TFQPNPVNRVICP-DAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS-------PILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
Y CCP + V C D +HCCP+ +C+L+ +C V SI +V+C D S+C D ++CC +G +ACCP K HCCP+ T C++ C S + + M K PA +S +++E +GSS +C TCC +G +GCCPF AVCC D + CCP+ CNL A TC+ +L K +F V P D C D TCCK G +GCCP+ A CC D IHCCP+ CN+ TC+ ++ + + +E + C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 129 YGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC--------------VKKESIKTVLCKDGVSECPDETTCCENQEGKWACCPMP-------KAVCCDDKIHCCPEETTCDVEQMKCLSSSDKAL-PMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSKVSVKMEDVPCDGSTSCPDQTTCCKTQEG-GWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFSNVSVKMEDVPCDGSTSCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 522 HSP 8 Score: 137.117 bits (344), Expect = 3.038e-34 Identity = 99/369 (26.83%), Postives = 159/369 (43.09%), Query Frame = 2
Query: 407 VPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSK------LKEVVTKMKLKIPAISNL-SIECPDGSSTCKTGATC-----CLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC----------------------DHQAEALEIKI-------------------SPILVES-----------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN-----ILLKFPIIN 1306
V + + + +CP+ +C+ G +CC ++ CCP+ A CC D HCCP++ +C+++ +C + ++ V +++ + + S D + C + C CL + YGCCP + V C D CCP+ C+L + +C KK S + V+C D +C D TCC+ G + CCP A CC DKIHCCP+ T C++ + C ++Q E E+ P +E I C + C + TCC + + CCP+P AVCC D HCCP +C+L ++C+ + +L K P +
Sbjct: 10 VSLALVAADMCPNGGKCEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDNICPDQSRCPAEFSCLKALTRYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC---VKKES----------IKTVLCKDGVSECPDETTCCENQEG-KWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFPARTRADWENQKEGKEVSAVAVEGNETKNFPEVTTANPVPAFLEGSSVAGEASAKDIPCNETASCPDQTTCCKTQEGGWGCCPFPEAVCCEDFIHCCPKGKTCNLAAQTCEGDGGSVPMLEKVPSFS 364 HSP 9 Score: 105.531 bits (262), Expect = 7.783e-24 Identity = 71/234 (30.34%), Postives = 109/234 (46.58%), Query Frame = 2
Query: 665 CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKT----------------KKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
CP+G C+ G TCC Y CCPF A CC D + CCP++ +C++ +C + T K +I+++ + ICPD + +C +C K T YGCCP + C D HCCP+ C+++ +C + ++++ C +G S C + TCC + ++CCP P AVCC D HCCP T+C + C + P+ P
Sbjct: 20 CPNGGK-CEKGQTCCQSPSKDYDCCPFDEAECCGDHIHCCPKDMLCDMTTSSCGNATLSMRWVERVSANLQMATKSFRMIRSYMGEDEDN-ICPD-QSRCPAEFSCLKALT--RYGCCPLAQGVSCPDGKHCCPEGHQCSLDSRSC----------VKKESIKTVLCKDGVSECPDETTCCENQEGKWACCPMPKAVCCDDKIHCCPEETTCDVEQMKCLSSSDKALPMWAKFP 238 HSP 10 Score: 104.375 bits (259), Expect = 2.175e-23 Identity = 75/229 (32.75%), Postives = 100/229 (43.67%), Query Frame = 2
Query: 269 YSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILS-----VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSS----------------TCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI 892
+ CCP AVCC+D +HCCP + +C +C V EVV K+PA+ + V C QC + SCC + G + CCP +AVCC DK+HCCP+ TCD K + L++ + + PD S +C TCC S ++GCC AVCC D CCP C G+C T L TF N +I
Sbjct: 905 WGCCPLPKAVCCADGEHCCPADYKCKEEDTSC--VKGEVVIPWYTKIPATDSIQADSSFVQCSAVHQCPEHMSCCRLFTGEWGCCPLPNAVCCGDKEHCCPEGY-------TCDLASKSCHKLLTLQLETVPLTPVFLPDDQSQHGPVKPILNRCDDVYSCNVDETCCRTSHTTWGCCLSPKAVCCRDMKHCCPDGYRCTK-GGSCIKNTGLHWLNWHTFFTNKRKAII 1123
BLAST of Granulin precursor vs. Ensembl Medaka
Match: ENSORLT00000005071.2 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 104.375 bits (259), Expect = 8.173e-25 Identity = 86/285 (30.18%), Postives = 117/285 (41.05%), Query Frame = 2
Query: 623 MKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPS----LLIKTFQPNP------VNRV---------------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--------------------------DHQAEALEIKISPILVES--------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPI 1300
+ L + + SIECPDG C +TCCL G + CCP AVCCSDK CCP CNL C+ KT +P +L K P N V + D+ Y C D TCC+ P G ++ CCPY CC D HCCP C++ C + + LE ++ + S I+C + C +CC A ++CCPYP CC+D HCC +C + +C K L +P+
Sbjct: 12 VGLSVCGFAFCSIECPDGK-LCPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCE-KTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKG-AWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRK---LSYPV 289 HSP 2 Score: 103.99 bits (258), Expect = 1.099e-24 Identity = 84/274 (30.66%), Postives = 112/274 (40.88%), Query Frame = 2
Query: 395 IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVV-----TKMKLKIPAISNLS-------------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC------------DSKTKKPSLLIKTFQPNPV------------------NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC 1054
+ L V + S+ CPD C D S+CC+ G F+CCP AVCCSDK HCCP CNL+ C+ + V TK + P +LS ++C D C TCC G++ CCP+ CC D CCP C+L C + T P+ LI + N + + VI D+K+ C G +CCK G + CCPY CC+D HCC C+ TC
Sbjct: 12 VGLSVCGFAFCSIECPDGKLCPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQC-DSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEG-QWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 282 HSP 3 Score: 86.6557 bits (213), Expect = 1.134e-18 Identity = 70/243 (28.81%), Postives = 96/243 (39.51%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-----VLKEVVTKIKLKVPASSILS-------------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----------------------DSKLKEVVTKMKLKIPAISNLS--------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC 823
T +SCCP AVCCSDK HCCP CNL C+ V ++TK + P + LS V C C D ++CC G + CCPY CC D HCCP C+L+ C S +E +++ + A++ S I C D C G +CC ++G + CCP+ CC+D CC C+ + TC
Sbjct: 41 TGHGFSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRC-DSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 282
BLAST of Granulin precursor vs. Ensembl Medaka
Match: ENSORLT00000029765.1 (granulins [Source:NCBI gene;Acc:101168633]) HSP 1 Score: 103.99 bits (258), Expect = 1.022e-24 Identity = 84/274 (30.66%), Postives = 112/274 (40.88%), Query Frame = 2
Query: 395 IKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVV-----TKMKLKIPAISNLS-------------------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC------------DSKTKKPSLLIKTFQPNPV------------------NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC 1054
+ L V + S+ CPD C D S+CC+ G F+CCP AVCCSDK HCCP CNL+ C+ + V TK + P +LS ++C D C TCC G++ CCP+ CC D CCP C+L C + T P+ LI + N + + VI D+K+ C G +CCK G + CCPY CC+D HCC C+ TC
Sbjct: 10 VGLSVCGFAFCSIECPDGKLCPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQC-DSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEG-QWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 280 HSP 2 Score: 102.834 bits (255), Expect = 2.206e-24 Identity = 84/277 (30.32%), Postives = 113/277 (40.79%), Query Frame = 2
Query: 623 MKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPS----LLIKTFQPNP------VNRV---------------ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC--------------------------DHQAEALEIKISPILVES--------IKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK 1276
+ L + + SIECPDG C +TCCL G + CCP AVCCSDK CCP CNL C+ KT +P +L K P N V + D+ Y C D TCC+ P G ++ CCPY CC D HCCP C++ C + + LE ++ + S I+C + C +CC A ++CCPYP CC+D HCC +C + +C K
Sbjct: 10 VGLSVCGFAFCSIECPDGK-LCPDLSTCCLTGHG-FSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCE-KTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKG-AWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRCDSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATCRK 282 HSP 3 Score: 86.6557 bits (213), Expect = 1.156e-18 Identity = 70/243 (28.81%), Postives = 96/243 (39.51%), Query Frame = 2
Query: 257 TDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-----VLKEVVTKIKLKVPASSILS-------------------VVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----------------------DSKLKEVVTKMKLKIPAISNLS--------IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC 823
T +SCCP AVCCSDK HCCP CNL C+ V ++TK + P + LS V C C D ++CC G + CCPY CC D HCCP C+L+ C S +E +++ + A++ S I C D C G +CC ++G + CCP+ CC+D CC C+ + TC
Sbjct: 39 TGHGFSCCPLPEAVCCSDKAHCCPSGYRCNLATMMCEKTDQPWVSLPMLTKEAAEEPPALDLSTNLVEELKNDNGLAQKSTGVQCDSFYMCPDRTTCCRHPKGAWFCCPYYPGRCCLDGFHCCPYGYDCDLTYTHCIRDGFKYPFYYKQALTSVPAALISHPEENSIQLEKSLTALTEASDAPSEHGVIRC-DSKFFCPAGTSCCKGAEGQWTCCPYPLGKCCADGRHCCQYGYTCDATSATC 280
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000029882.1 (SMESG000029882.1) HSP 1 Score: 655.596 bits (1690), Expect = 0.000e+0 Identity = 332/351 (94.59%), Postives = 342/351 (97.44%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 1315
GSY+CCPYSSAVCCSDK+HCCPQNT CNL+ GTCDS LKEVVTK+KLKVPASSILSVVCPDQSQC+DGS+CC +A G++ACCPYSSAVCCSDK+HCCPQNTVCNL AGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNL AGTCDSKTKKPSLLIKTFQPNPVNRVICP AKYQCSDGQTCCKLP GDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP
Sbjct: 364 GSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKVPASSILSVVCPDQSQCNDGSTCCAVAGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLSAGTCDSKTKKPSLLIKTFQPNPVNRVICPGAKYQCSDGQTCCKLPAGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPIINFHP 714 HSP 2 Score: 535.028 bits (1377), Expect = 0.000e+0 Identity = 302/413 (73.12%), Postives = 339/413 (82.08%), Query Frame = 2
Query: 86 MWKFIFVVLISSVVCHDVYRFSREFIKPITETNKIPVKVVNXXXXXXXXXXXXXXVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQP-NPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA----LEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD---KNILLKFPI 1300
MWKFIFVVLISSVVCHDVYRFSREFIKPITETNKIP KVVNCPDDSVCDDGSSCCVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGN+ACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLK+PA S LS+ CPD S C G+TCC ++ GSY CCP+S+AVCCSDK CCPQNTVCNL AGTCDSK K+ +K P + + V+CPD + QC+DG TCC++ G SY CCPYS+A CCSDK HCCPQNTVCN++ GTCD + + +++K+ + S+ CP+ S C TCC A SY+CCPY +AVCCSD HCCP NT C+L+ +CD K ++ K +
Sbjct: 1 MWKFIFVVLISSVVCHDVYRFSREFIKPITETNKIPAKVVNCPDDSVCDDGSSCCVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNYACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKVPASSILSVVCPD-QSQCNDGSTCCEVAGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKVPASSILSVVCPD-QSQCNDGSTCCEV-AGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKVPASSILSVVCPDQSQCNDGSTCCAVAGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKL 410 HSP 3 Score: 406.371 bits (1043), Expect = 1.664e-135 Identity = 228/343 (66.47%), Postives = 258/343 (75.22%), Query Frame = 2
Query: 263 GSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI-CPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAE--ALEIK-ISPILVESIKCPNGSL-CGQKGTCCP-SANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
GSY+CCPYSSAVCCSDK+HCCPQNT CNL+ GTCDS LKEVVTK+KLKVPASSILSVVCPDQSQC+DGS+CC +A G++ACCPYSSAVCCSDK+HCCPQNTVCNL AGTCDSKLKEVVTKMKLK+PA S LS+ CPD S C G+TCC ++ GSY CCP+S+AVCCSDK CCPQNTVCNL AGTCDSK K+ +K P N I CPD C G TCC L G SYGCCP+S A CCSDK+ CCPQNTVCN++ GTCD + + +L IK P V + CP C TCC A DSY CCPY A CCSD HCCP NT C++N +CD
Sbjct: 288 GSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKVPASSILSVVCPDQSQCNDGSTCCAVAGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKVPASSILSVVCPD-QSQCNDGSTCCAVAGGSYACCPYSSAVCCSDKRHCCPQNTVCNLDAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDG-SYGCCPFSNAVCCSDKLSCCPQNTVCNLSAGTCDSKTKKPSLLIKTFQPNPVNRVICPGAKYQCSDGQTCCKLPAGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD 628 HSP 4 Score: 176.022 bits (445), Expect = 3.267e-48 Identity = 106/209 (50.72%), Postives = 135/209 (64.59%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGN-FACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQP 871
+ +DGSY CCP+S+AVCCSDK CCPQNT CNL+ GTCDS K+ IK P + + V+CP + QC DG +CC + G+ + CCPYS+A CCSDK HCCPQNTVCN++ GTCD + + +++KI I SI+CP+G S C TCC ++ SY CCP+ NAVCCSD CCP NT C+L +CD K I F P
Sbjct: 513 LLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLSAGTCDSKTKKPSLLIKTFQP-NPVNRVICPGAKYQCSDGQTCCKLPAGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA----LEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD-KNILLKFPIINFHP 714
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 205.297 bits (521), Expect = 4.472e-61 Identity = 139/431 (32.25%), Postives = 209/431 (48.49%), Query Frame = 2
Query: 95 FIFVVLISSVVCHDVYRFSREFIKPITETNKIPVKVVNXXXXXXXXXXXXXXVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS---------VLKEVVTKIKLKVPASSIL--------------SVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC-----------DSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ---------AEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
I +++ + ++ F++ ++E N+ V + + C + +CCVT SY CC Y +A+CC DK HCCP + C+ K C V++E + L + SS L + CP+ +++C D +CC+ GN+ CCP+ +AVCCSDK +CCP+NT C+L C + LK + + L +CP+ C CC +S G +GCC F+ +CC D +CCP NT C+ C SK+ + + QP ++ ICPD +C +TCC + G YGCCP+ ATCCSD CCP N VC++ K C ++ +++L S I ++ I CP+ + C CC ++ Y CC Y N VCC+D CCPN T C
Sbjct: 7 MILTIIVDYALTDGIFSFNKASQDQVSEKNE----NVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDG-LYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 431 HSP 2 Score: 152.91 bits (385), Expect = 1.969e-41 Identity = 107/321 (33.33%), Postives = 160/321 (49.84%), Query Frame = 2
Query: 434 VCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----DSKLKEVVTKMKLKIPAIS-------------------NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKT-----KKPSL----LIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKISPILVESIKCPNGSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
VCP+ +++C + +CCV ++ CC Y +A+CC DK HCCP + C+ + C + ++ V + L + + N I CP+ + CK TCC+ G+Y CCP NAVCCSDK +CCP+NT C+L C DS K P L L + V+++ CP+++ QC + CC + +G +GCC ++ CC D CCP NT C+ K C ++ L E++ + + CP+ + C TCC + Y CCP+ NA CCSD++ CCP+N C L K C
Sbjct: 39 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPM-SGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC 358 HSP 3 Score: 97.8265 bits (242), Expect = 6.697e-22 Identity = 72/243 (29.63%), Postives = 105/243 (43.21%), Query Frame = 2
Query: 665 CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTK-------------------KPSLLIKTFQPNPV-----NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL-------------VESI--KCPNGS-LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
CP+ + CK TCC+ SY CC + NA+CC DK CCP + C+ C + KPS + F+ N + + CP+ K +C D +TCC G+ Y CCP+ A CCSDK +CCP+NT C++ C + +P L V+ + KCPN C + CCP + + CC + +CC P+ +C + CD
Sbjct: 40 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYY-CCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICC-------PDMKTCCPHNTDCD 274 HSP 4 Score: 88.1965 bits (217), Expect = 1.045e-18 Identity = 59/163 (36.20%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 866 QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---------------LEIKISPILV------------ESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
Q + N +CP+ + +C + QTCC + +SY CC Y A CC DK HCCP + C+ K C E L++K S L + I CPN + C TCC + + +Y CCP+ NAVCCSD +CCP NT C L K C
Sbjct: 31 QVSEKNENVCPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 192 HSP 5 Score: 56.6102 bits (135), Expect = 1.964e-8 Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 2
Query: 1115 CPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL--LKFPII 1303
CPN + C + TCC + ++SY CC YPNA+CC D HCCP+ ++C K C +N+ +++P++
Sbjct: 40 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVV 105
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 205.297 bits (521), Expect = 4.878e-61 Identity = 139/431 (32.25%), Postives = 209/431 (48.49%), Query Frame = 2
Query: 95 FIFVVLISSVVCHDVYRFSREFIKPITETNKIPVKVVNXXXXXXXXXXXXXXVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS---------VLKEVVTKIKLKVPASSIL--------------SVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC-----------DSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ---------AEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
I +++ + ++ F++ ++E N+ V + + C + +CCVT SY CC Y +A+CC DK HCCP + C+ K C V++E + L + SS L + CP+ +++C D +CC+ GN+ CCP+ +AVCCSDK +CCP+NT C+L C + LK + + L +CP+ C CC +S G +GCC F+ +CC D +CCP NT C+ C SK+ + + QP ++ ICPD +C +TCC + G YGCCP+ ATCCSD CCP N VC++ K C ++ +++L S I ++ I CP+ + C CC ++ Y CC Y N VCC+D CCPN T C
Sbjct: 1 MILTIIVDYALTDGIFSFNKASQDQVSEKNE----NVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDG-LYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 425 HSP 2 Score: 152.525 bits (384), Expect = 2.656e-41 Identity = 111/332 (33.43%), Postives = 164/332 (49.40%), Query Frame = 2
Query: 434 VCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----DSKLKEVVTKMKLKIPAIS-------------------NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKT-----KKPSL----LIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKISPILVESIKCPNGSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNILLKFPII 1303
VCP+ +++C + +CCV ++ CC Y +A+CC DK HCCP + C+ + C + ++ V + L + + N I CP+ + CK TCC+ G+Y CCP NAVCCSDK +CCP+NT C+L C DS K P L L + V+++ CP+++ QC + CC + +G +GCC ++ CC D CCP NT C+ K C ++ L E++ + + CP+ + C TCC + Y CCP+ NA CCSD++ CCP+N C L K C KN P I
Sbjct: 33 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPM-SGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC-KNKETSQPSI 362 HSP 3 Score: 87.8113 bits (216), Expect = 1.380e-18 Identity = 59/163 (36.20%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 866 QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---------------LEIKISPILV------------ESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
Q + N +CP+ + +C + QTCC + +SY CC Y A CC DK HCCP + C+ K C E L++K S L + I CPN + C TCC + + +Y CCP+ NAVCCSD +CCP NT C L K C
Sbjct: 25 QVSEKNENVCPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 186
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018770.1 (SMESG000018770.1) HSP 1 Score: 205.297 bits (521), Expect = 5.284e-61 Identity = 139/431 (32.25%), Postives = 209/431 (48.49%), Query Frame = 2
Query: 95 FIFVVLISSVVCHDVYRFSREFIKPITETNKIPVKVVNXXXXXXXXXXXXXXVTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS---------VLKEVVTKIKLKVPASSIL--------------SVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC-----------DSKLKEVVTKMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQ---------AEALEIKISPILVESIKCPNGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSC 1249
I +++ + ++ F++ ++E N+ V + + C + +CCVT SY CC Y +A+CC DK HCCP + C+ K C V++E + L + SS L + CP+ +++C D +CC+ GN+ CCP+ +AVCCSDK +CCP+NT C+L C + LK + + L +CP+ C CC +S G +GCC F+ +CC D +CCP NT C+ C SK+ + + QP ++ ICPD +C +TCC + G YGCCP+ ATCCSD CCP N VC++ K C ++ +++L S I ++ I CP+ + C CC ++ Y CC Y N VCC+D CCPN T C
Sbjct: 10 MILTIIVDYALTDGIFSFNKASQDQVSEKNE----NVCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDG-LYGCCPFKNATCCSDNASCCPHNKVCDLEKKVCKNKETSQPSITKSKSLLHFYSEIGLKVI-CPDQTKCENDSVCCQMDDNQYGCCSYKNGVCCADKQTCCPNGTQC 434 HSP 2 Score: 152.91 bits (385), Expect = 2.396e-41 Identity = 107/321 (33.33%), Postives = 160/321 (49.84%), Query Frame = 2
Query: 434 VCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC----DSKLKEVVTKMKLKIPAIS-------------------NLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKT-----KKPSL----LIKTFQPNPVNRV--ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEAL-----EIKISPILVESIKCPNGSL-CGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
VCP+ +++C + +CCV ++ CC Y +A+CC DK HCCP + C+ + C + ++ V + L + + N I CP+ + CK TCC+ G+Y CCP NAVCCSDK +CCP+NT C+L C DS K P L L + V+++ CP+++ QC + CC + +G +GCC ++ CC D CCP NT C+ K C ++ L E++ + + CP+ + C TCC + Y CCP+ NA CCSD++ CCP+N C L K C
Sbjct: 42 VCPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPM-SGGHFGCCKFNKGICCPDMKTCCPHNTDCDSAKKACVSKSSDLTEIPNELQPEAQIKLNNICPDKHVECQSFETCCSVGDGLYGCCPFKNATCCSDNASCCPHNKVCDLEKKVC 361 HSP 3 Score: 97.8265 bits (242), Expect = 7.730e-22 Identity = 72/243 (29.63%), Postives = 105/243 (43.21%), Query Frame = 2
Query: 665 CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTK-------------------KPSLLIKTFQPNPV-----NRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKISPIL-------------VESI--KCPNGS-LCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCD 1273
CP+ + CK TCC+ SY CC + NA+CC DK CCP + C+ C + KPS + F+ N + + CP+ K +C D +TCC G+ Y CCP+ A CCSDK +CCP+NT C++ C + +P L V+ + KCPN C + CCP + + CC + +CC P+ +C + CD
Sbjct: 43 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYY-CCPHKNAVCCSDKYNCCPENTKCDLKNKKCLDSSAVFNSYKNPGLKSANLYTFYSLLNVDKLEKKCPNSEKQCPGQKKCCPMSGGHFGCCKFNKGICC-------PDMKTCCPHNTDCD 277 HSP 4 Score: 88.1965 bits (217), Expect = 1.092e-18 Identity = 59/163 (36.20%), Postives = 78/163 (47.85%), Query Frame = 2
Query: 866 QPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEA---------------LEIKISPILV------------ESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
Q + N +CP+ + +C + QTCC + +SY CC Y A CC DK HCCP + C+ K C E L++K S L + I CPN + C TCC + + +Y CCP+ NAVCCSD +CCP NT C L K C
Sbjct: 34 QVSEKNENVCPNNRTKCKETQTCC-VTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVVEENLPVKMLDLKPSSTLHGFESNSETLRVNDDITCPNNKTKCKDTKTCCITKHGNYYCCPHKNAVCCSDKYNCCPENTKCDLKNKKC 195 HSP 5 Score: 56.6102 bits (135), Expect = 1.958e-8 Identity = 27/66 (40.91%), Postives = 43/66 (65.15%), Query Frame = 2
Query: 1115 CPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNIL--LKFPII 1303
CPN + C + TCC + ++SY CC YPNA+CC D HCCP+ ++C K C +N+ +++P++
Sbjct: 43 CPNNRTKCKETQTCCVTKHNSYHCCRYPNAICCPDKYHCCPSGSTCDAAKKRCTRNVEGPMEWPVV 108
BLAST of Granulin precursor vs. Planmine SMEST
Match: SMESG000018760.1 (SMESG000018760.1) HSP 1 Score: 186.808 bits (473), Expect = 1.811e-52 Identity = 127/407 (31.20%), Postives = 195/407 (47.91%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIK-----LKVPASS------ILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC------DSKLKEVVTKMKLK-------IPAISNLSIE--------CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI-------------CPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC---------------------DHQAEALEIKISPILVESIKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC 1270
T SY CC Y++AVCC DK HCCP T C+ C+ ++ + I+ +++P I CP+ +++C D +CCV G++ CC +++AVCC DK HCCP+ + C+ C + ++++++ +K++ + A N E CP+ + C TCC SY CC ++NAVCC DK CCP T C+ C+ + I+T P P+ + CP+ + +C D QTCC G SY CC ++ A CC DK HCCP+ + C+ + C + + LE K++ + + CPN + C TCC + +++Y CCP+ NAVCCSD CCP T+C + K C
Sbjct: 33 TKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQT-TPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHG-SYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNE--KDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKC 435 HSP 2 Score: 176.407 bits (446), Expect = 1.160e-48 Identity = 123/397 (30.98%), Postives = 188/397 (47.36%), Query Frame = 2
Query: 254 TTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDSVLKEVVTKIK-----LKVPASS------ILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTC------DSKLKEVVTKMKLKIPAISNL---------SIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDA------------KYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTC-----DHQAEALEIKISPILVESIKCP-NGSLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSC---DKNILLKFPI 1300
T SY CC Y++AVCC DK HCCP T C+ C+ ++ + I+ +++P I CP+ +++C D +CCV G++ CC +++AVCC DK HCCP+ + C+ C + ++ +++ +K++ +N+ + CP+ + CK TCC+ +Y CCP NAVCCSDK CCP+ T C++ C S + ++ + K + S C + G S G C +SA TCCSD CCPQ+T C+ C D E + + + ++ CP N + C TCC ++ SY CC NAVCC+D CCP+N C + T SC + N FP+
Sbjct: 219 TKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKVNEKDEVMCPNNKTKCKDTKTCCITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRS-GRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPL 614 HSP 3 Score: 173.326 bits (438), Expect = 1.503e-47 Identity = 123/411 (29.93%), Postives = 195/411 (47.45%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTC------DSVLKEVVTKIKLK-VPASSILSV--------------VCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK-----LKIPAISNLSIE------CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCN-------------------LPAGTCDSKTKKPSLLIKTFQPNPVNRVICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCD---HQAEALEIKISPILV-ESIKCPNGS------LCGQKGTC------CPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKN 1279
VT GSY CC +++AVCC DK HCCP+ + C+ + C + +++++ +K++ P ++IL +CP+ +++C + +CC ++ CC Y++AVCC DK HCCP T C+ C+ ++ + ++ +++P I ++ CP+ + C+ TCC+ GSY CC F+NAVCC DK CCP+ + C+ +PA +++ K L K N + V+CP+ K +C D +TCC + ++Y CCP+ A CCSDK CCP+ T C+I C H + +++ L+ +S K L G K CP N C + CCSD CCP +T C C N
Sbjct: 120 VTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMGKLIPALKVENELKTNILEAKV---NEKDEVMCPNNKTKCKDTKTCC-ITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLN 526 HSP 4 Score: 164.851 bits (416), Expect = 1.539e-44 Identity = 107/345 (31.01%), Postives = 165/345 (47.83%), Query Frame = 2
Query: 392 KIKLKVPASSILSVVCPD-QSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMK-----LKIPAISNLSIE------CPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCN-------------------LPAGTCDSKTKKPSLLIKTFQPNPVNRV---ICPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAEALEIKIS--------PILVES-------IKCPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDK 1276
K+ P + +CP+ +++C + +CC ++ CC Y++AVCC DK HCCP T C+ C+ ++ + ++ +++P I ++ CP+ + C+ TCC+ GSY CC F+NAVCC DK CCP+ + C+ +PA + + K L K + + ICP+ + +C++ QTCC+ +SY CC Y+ A CC DK HCCP T C+ C+ EA + I P++ E+ CPN + C TCC + + SY CC + NAVCC D HCCP ++C C K
Sbjct: 2 KLNKSAPNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQT-KHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQTTPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHGSYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVK 345 HSP 5 Score: 130.183 bits (326), Expect = 2.121e-32 Identity = 92/267 (34.46%), Postives = 126/267 (47.19%), Query Frame = 2
Query: 620 KMKLKIPAISNLSIECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTCDSKTKKPSLLIKTFQPNPVNRVI-------------CPDAKYQCSDGQTCCKLPTGDSYGCCPYSAATCCSDKIHCCPQNTVCNINKGTCDHQAE----------ALEIKISP---ILVESIK------------CPNG-SLCGQKGTCCPSANDSYSCCPYPNAVCCSDHSHCCPNNTSCSLNTKSCDKNI-LLKFPI 1300
K+ P + CP+ + C TCC SY CC ++NAVCC DK CCP T C+ C+ + I+T P P+ + CP+ + +C D QTCC G SY CC ++ A CC DK HCCP+ + C+ + C A+ AL+++ P IL + CPN + C ++ TCC + ++SY CC Y NAVCC D HCCPN TSC K C++NI K PI
Sbjct: 2 KLNKSAPNKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPIQT-TPEPMEMPVIEETDVKIITDQTCPNNRTKCQDSQTCCVTKHG-SYHCCKFANAVCCPDKFHCCPEGSTCDAKQHKCVKCAKSNVEMRKLIPALKVEDEPKTNILEAKVNEKDENKETVGNICPNKRTKCTERQTCCQTKHNSYHCCKYANAVCCPDKFHCCPNGTSCDSVHKKCNRNIEATKSPI 266 HSP 6 Score: 120.553 bits (301), Expect = 4.159e-29 Identity = 74/234 (31.62%), Postives = 115/234 (49.15%), Query Frame = 2
Query: 251 VTTDGSYSCCPYSSAVCCSDKQHCCPQNTECNLNKGTCDS-------VLKEVVTKI-----KLKVPASSILSVVCPDQSQCDDGSSCCVMADGNFACCPYSSAVCCSDKQHCCPQNTVCNLSAGTCDSKLKEVVTKMKLKIPAISNLS-IECPDGSSTCKTGATCCLLSDGSYGCCPFSNAVCCSDKLSCCPQNTVCNLPAGTC-DSKTKKPSLLIKTFQPNPVNRVICPDAKY 910
+T +Y CCP+ +AVCCSDK CCP+ T C++ C S + E+ ++ K + I+S++ + + C + +G C +S+ CCSD + CCPQ+T C+ C + T+ P + LS + CPD + C + TCC +SD SYGCC NAVCC+D++SCCP N C++ +C ++ + K TF R D +
Sbjct: 398 ITKHNNYYCCPHKNAVCCSDKYQCCPEATTCDIKNKKCTSSPHSIHEIAHEMAQELIHDSFKYETNFFKIISLLLGFKQKASKSDFYCPITNGRSGRCKFSAGTCCSDLRTCCPQHTKCDEKNNKCTLNSTDFDTEKSKHSPNTNRLSTVVCPDNETECFSWETCCAVSDKSYGCCELENAVCCADRVSCCPHNQKCDIKTNSCVETNSNKRRHFPLTFPKRSKFRKTLNDGNH 631 The following BLAST results are available for this feature:
BLAST of Granulin precursor vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 3
BLAST of Granulin precursor vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Granulin precursor vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 4
BLAST of Granulin precursor vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of Granulin precursor vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Granulin precursor vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Granulin precursor vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of Granulin precursor vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Granulin precursor vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 1
BLAST of Granulin precursor vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Granulin precursor vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011250 ID=SMED30011250|Name=Granulin precursor|organism=Schmidtea mediterranea sexual|type=transcript|length=1335bpback to top protein sequence of SMED30011250-orf-1 >SMED30011250-orf-1 ID=SMED30011250-orf-1|Name=SMED30011250-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=411bp MWKFIFVVLISSVVCHDVYRFSREFIKPITETNKIPVKVVNCPDDSVCDDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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