SMED30011202
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011202 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 4
Homology
BLAST of SMED30011202 vs. Planmine SMEST
Match: SMESG000022960.1 (SMESG000022960.1) HSP 1 Score: 621.313 bits (1601), Expect = 0.000e+0 Identity = 441/441 (100.00%), Postives = 441/441 (100.00%), Query Frame = 2 Query: 143 MNNTGHILDNNIDSSHMVNXXXXXXXXXXXXXXXXXXXGLEYESTSQATSTPKESNSPPKLRSNLDEVIELVIQDAIEYADAPEEKRILPALTRKSTKAXXXXXXXXXXXXXXXFTNVASPQQQLAPMNSSGLLMSANASNNPGSFHPLVQANLPPGMQHMLQLATTTKPGISMGGSNTPTSLXXXXXXXXXXXXXMASAMMGFSAAQLPNMIMPSYNTNLQSNYSQQTPGGQYQLPVXXXXXXXXXXXXXXXXXXGNNPNSFTDLSSTSGSQQNINFGNGISNANQQLLQSSYGNFGANSGISVSPYQIPNMIQAYTQSPTVSDNTNPNPISQNDSINSPVNLNSNPTSASCFHSSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPFELQQTQIYAHHRLLXXXXXXXSNNNTAGLFPDYAAVMARYGTYH 1465 MNNTGHILDNNIDSSHMVNKDHDIIHDDDLDDDFKKDIGLEYESTSQATSTPKESNSPPKLRSNLDEVIELVIQDAIEYADAPEEKRILPALTRKSTKAKVKTISVNSSSSINTFTNVASPQQQLAPMNSSGLLMSANASNNPGSFHPLVQANLPPGMQHMLQLATTTKPGISMGGSNTPTSLQHQNNTTQQQQQQMASAMMGFSAAQLPNMIMPSYNTNLQSNYSQQTPGGQYQLPVSLSQIQLAQQQQSSHLLLGNNPNSFTDLSSTSGSQQNINFGNGISNANQQLLQSSYGNFGANSGISVSPYQIPNMIQAYTQSPTVSDNTNPNPISQNDSINSPVNLNSNPTSASCFHSSKANNNNNALTLNSIVSNNQNISTTSSAAAAAASLYQNNPFELQQTQIYAHHRLLQAQQQQQSNNNTAGLFPDYAAVMARYGTYH Sbjct: 1 MNNTGHILDNNIDSSHMVNKDHDIIHDDDLDDDFKKDIGLEYESTSQATSTPKESNSPPKLRSNLDEVIELVIQDAIEYADAPEEKRILPALTRKSTKAKVKTISVNSSSSINTFTNVASPQQQLAPMNSSGLLMSANASNNPGSFHPLVQANLPPGMQHMLQLATTTKPGISMGGSNTPTSLQHQNNTTQQQQQQMASAMMGFSAAQLPNMIMPSYNTNLQSNYSQQTPGGQYQLPVSLSQIQLAQQQQSSHLLLGNNPNSFTDLSSTSGSQQNINFGNGISNANQQLLQSSYGNFGANSGISVSPYQIPNMIQAYTQSPTVSDNTNPNPISQNDSINSPVNLNSNPTSASCFHSSKANNNNNALTLNSIVSNNQNISTTSSAAAAAASLYQNNPFELQQTQIYAHHRLLQAQQQQQSNNNTAGLFPDYAAVMARYGTYH 441
BLAST of SMED30011202 vs. Planmine SMEST
Match: SMESG000074461.1 (SMESG000074461.1) HSP 1 Score: 128.257 bits (321), Expect = 7.038e-33 Identity = 97/224 (43.30%), Postives = 129/224 (57.59%), Query Frame = 2 Query: 257 GLEYESTSQATSTPKESNSPPKLRSNLDEVIELVIQDAIEYADAPEEKRILPALTRKSTKAXXXXXXXXXXXXXXXFTNVASPQQQLAPMNSSGLLMSANASNNPG-------SFHPLVQANLP-PGMQHM---LQLATTTKPGIS-------------MGGSNTPTSLXXXXXXXXXXXXXMASAMMGFSAAQLPNMIMPSYNTNLQSNYSQQTPGGQYQLPV 856 GLE+++ SQ +S++ PK RSNLDEVI+ VIQDA++YADAP EKRILP L RKSTK++ + +N+ + Q M +S +A + NPG + HPL+QAN P MQ M LQLA T K +S +GG+ T T+ N +QQ QQQ+ +AMMGF A+Q+PNM+MP+YN N+ Y QQ GQY LPV Sbjct: 31 GLEFDNGSQT----NDSSTTPKPRSNLDEVIDSVIQDALDYADAPPEKRILPPLVRKSTKSRSRQ------------SNIQNHQ-----MTTSNHTANALMTQNPGSNNSNNNAIHPLMQANFAHPNMQQMAAQLQLAATGKGIVSGTNNNSNLMQHNMIGGTGTATN-----NQSQQHQQQLTAAMMGFGASQIPNMMMPTYNGNVPITYHQQMTSGQYHLPV 228
BLAST of SMED30011202 vs. Planmine SMEST
Match: SMESG000074461.1 (SMESG000074461.1) HSP 1 Score: 126.716 bits (317), Expect = 2.639e-32 Identity = 96/224 (42.86%), Postives = 127/224 (56.70%), Query Frame = 2 Query: 257 GLEYESTSQATSTPKESNSPPKLRSNLDEVIELVIQDAIEYADAPEEKRILPALTRKSTKAXXXXXXXXXXXXXXXFTNVASPQQQLAPMNSSGLLMSANASNNPG-------SFHPLVQANLP-PGMQHM---LQLATTTKPGIS-------------MGGSNTPTSLXXXXXXXXXXXXXMASAMMGFSAAQLPNMIMPSYNTNLQSNYSQQTPGGQYQLPV 856 GLE+++ SQ +S++ PK RSNLDEVI+ VIQDA++YADAP EKRILP L RKSTK++ + +N Q +++ LM + NPG + HPL+QAN P MQ M LQLA T K +S +GG+ T T+ N +QQ QQQ+ +AMMGF A+Q+PNM+MP+YN N+ Y QQ GQY LPV Sbjct: 31 GLEFDNGSQT----NDSSTTPKPRSNLDEVIDSVIQDALDYADAPPEKRILPPLVRKSTKSRSRQ------------SNSIQNHQMTTSNHTANALM----TQNPGSNNSNNNAIHPLMQANFAHPNMQQMAAQLQLAATGKGIVSGTNNNSNLMQHNMIGGTGTATN-----NQSQQHQQQLTAAMMGFGASQIPNMMMPTYNGNVPITYHQQMTSGQYHLPV 229 The following BLAST results are available for this feature:
BLAST of SMED30011202 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30011202 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30011202 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30011202 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30011202 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 3
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011202 ID=SMED30011202|Name=SMED30011202|organism=Schmidtea mediterranea sexual|type=transcript|length=1905bpback to top protein sequence of SMED30011202-orf-1 >SMED30011202-orf-1 ID=SMED30011202-orf-1|Name=SMED30011202-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=442bp MNNTGHILDNNIDSSHMVNKDHDIIHDDDLDDDFKKDIGLEYESTSQATSback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
|