Fzd-4-3

Overview
NameFzd-4-3
Smed IDSMED30011165
Length (bp)496
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Fzd-4-3 (SMED30011165) t-SNE clustered cells

Violin plots show distribution of expression levels for Fzd-4-3 (SMED30011165) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Fzd-4-3 (SMED30011165) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Fzd-4-3 (SMED30011165) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30011165

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior region of the whole animalSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016		whole organism asexual adult colorimetric in situ hybridization evidence
head regionSMED30011165h1SMcG0002271 SmedASXL_015764SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30011165h1SMcG0002271 dd_Smed_v4_13356_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30011165h1SMcG0002271 dd_Smed_v6_13356_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Fzd-4-3 vs. Ensembl Fly
Match: fz2 (gene:FBgn0016797 transcript:FBtr0330195)

HSP 1 Score: 46.9802 bits (110), Expect = 2.058e-6
Identity = 40/162 (24.69%), Postives = 64/162 (39.51%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSE-----QLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWL---------VNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKI 452
            G   PD L    + P    LV+G TFL  G               +I + + QQ       +   L  + +RI  F  +Y V  ++ I C  Y+   +  W+         V  P       P++ V +LK F+   VGI S +W  S  +  +W   +R++
Sbjct:  473 GNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFV----------SLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKK----PLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRL 620
[Close]
BLAST of Fzd-4-3 vs. Ensembl Fly
Match: fz2 (gene:FBgn0016797 transcript:FBtr0112890)

HSP 1 Score: 45.4394 bits (106), Expect = 7.967e-6
Identity = 41/167 (24.55%), Postives = 65/167 (38.92%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSE-----QLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWL---------VNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWD 467
            G   PD L    + P    LV+G TFL  G               +I + + QQ       +   L  + +RI  F  +Y V  ++ I C  Y+   +  W+         V  P       P++ V +LK F+   VGI S +W  S  +  +W   +R++    D
Sbjct:  473 GNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFV----------SLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKK----PLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRLLGAPD 625
[Close]
BLAST of Fzd-4-3 vs. Ensembl Fly
Match: fz2 (gene:FBgn0016797 transcript:FBtr0075029)

HSP 1 Score: 45.4394 bits (106), Expect = 7.967e-6
Identity = 41/167 (24.55%), Postives = 65/167 (38.92%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSE-----QLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWL---------VNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWD 467
            G   PD L    + P    LV+G TFL  G               +I + + QQ       +   L  + +RI  F  +Y V  ++ I C  Y+   +  W+         V  P       P++ V +LK F+   VGI S +W  S  +  +W   +R++    D
Sbjct:  473 GNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFV----------SLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKK----PLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRLLGAPD 625
[Close]
BLAST of Fzd-4-3 vs. Ensembl Fly
Match: fz2 (gene:FBgn0016797 transcript:FBtr0332735)

HSP 1 Score: 45.4394 bits (106), Expect = 7.967e-6
Identity = 41/167 (24.55%), Postives = 65/167 (38.92%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSE-----QLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWL---------VNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWD 467
            G   PD L    + P    LV+G TFL  G               +I + + QQ       +   L  + +RI  F  +Y V  ++ I C  Y+   +  W+         V  P       P++ V +LK F+   VGI S +W  S  +  +W   +R++    D
Sbjct:  473 GNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFV----------SLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKK----PLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRLLGAPD 625
[Close]
BLAST of Fzd-4-3 vs. Ensembl Fly
Match: fz2 (gene:FBgn0016797 transcript:FBtr0330194)

HSP 1 Score: 45.4394 bits (106), Expect = 7.967e-6
Identity = 41/167 (24.55%), Postives = 65/167 (38.92%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSE-----QLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWL---------VNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWD 467
            G   PD L    + P    LV+G TFL  G               +I + + QQ       +   L  + +RI  F  +Y V  ++ I C  Y+   +  W+         V  P       P++ V +LK F+   VGI S +W  S  +  +W   +R++    D
Sbjct:  473 GNLNPDHLKTFVLAPLFVYLVIGTTFLMAGFV----------SLFRIRSVIKQQGGVGAGVKADKLEKLMIRIGIFSVLYTVPATIVIGCYLYEAAYFEDWIKALACPCAQVKGPGKK----PLYSVLMLKYFMALAVGITSGVWIWSGKTLESWRRFWRRLLGAPD 625
[Close]
BLAST of Fzd-4-3 vs. Ensembl Xenopus
Match: fzd8 (frizzled class receptor 8 [Source:NCBI gene;Acc:100038274])

HSP 1 Score: 46.2098 bits (108), Expect = 8.603e-6
Identity = 37/147 (25.17%), Postives = 60/147 (40.82%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNN------PNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAW 431
            G Q  D L    + P +  L +G  FL  G               +I + + Q   +   L  + +RI  F  +Y V  ++ + C  Y+ +N   W V +      P       P + V++LK F+  VVGI S +W  S  +  +W
Sbjct:  397 GNQNLDNLRGFVLAPLVIYLFIGSMFLLAGFV----------SLFRIRSVIKQGGTKTDKLEKLMIRIGIFSVLYTVPATIVVACFFYEQHNRQGWEVAHNCNSCQPEVAQPRRPDYAVFMLKYFMCLVVGITSGVWIWSGKTLESW 533
[Close]
BLAST of Fzd-4-3 vs. TrEMBL
Match: A0A159X4Q9 (Frizzled-4-3 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=fz-4-3 PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.888e-26
Identity = 69/164 (42.07%), Postives = 88/164 (53.66%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS 494
            S GEQTPDLLL  SIIP++ CL++G  FL  G      R+ K S K     C +Q+   L+ +     R+  F  IY + I+  ICC+ YQ+ NY  W             I  V+LLK F+NQVVGI SAIW LSLDS  AW + F  + +K    KK   NS
Sbjct:   33 SVGEQTPDLLLTFSIIPQLICLIIGFIFLLSGFFFFKYRTKKFSNKS---LCHHQKITFLLSIG---FRLGLFSIIYIIAIATMICCELYQYYNYHYWSRAPIKFSKRKDAIVAVFLLKFFMNQVVGINSAIWILSLDSVAAWKKCFVMLLEKVTFNKKIKSNS 190
[Close]
BLAST of Fzd-4-3 vs. TrEMBL
Match: T1DBJ6 (Fzd-4-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.772e-24
Identity = 69/164 (42.07%), Postives = 88/164 (53.66%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS 494
            S GEQTPDLLL  SIIP++ CL++G  FL  G      R+ K S K     C +Q+   L+ +     R+  F  IY + I+  ICC+ YQ+ NY  W             I  V+LLK F+NQVVGI SAIW LSLDS  AW + F  + +K    KK   NS
Sbjct:  369 SVGEQTPDLLLTFSIIPQLICLIIGFIFLLSGFFFFKYRTKKFSNKS---LCHHQKITFLLSIG---FRLGLFSIIYIIAIATMICCELYQYYNYHYWSRAPIKFSKRKDAIVAVFLLKFFMNQVVGINSAIWILSLDSVAAWKKCFVMLLEKVTFNKKIKSNS 526
[Close]
BLAST of Fzd-4-3 vs. TrEMBL
Match: T1D142 (Fzd-4-3a (Fragment) OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 5.224e-17
Identity = 50/147 (34.01%), Postives = 77/147 (52.38%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELF 443
            S G Q+PD L+  SIIPK+ CL+LG+  L +G        +K+    Q +   +  S      S +  RI  F  IY     +SI C+ YQ+ NY  W +      +   P+  ++LL +F+NQ+VGI S++W  SLD+  +W + F
Sbjct:  382 SVGGQSPDRLMTFSIIPKLVCLILGMFLLFVGFIFLKPEEEKMKSNSQNIFRWSTSST-----SKVSCRIGLFSCIYVTATFISIVCEFYQYVNYVRWKIQLKIEESTAQPLVAIFLLNIFMNQIVGINSSVWIFSLDAAKSWRKFF 523
[Close]
BLAST of Fzd-4-3 vs. TrEMBL
Match: T1E111 (Fzd-4-3b OS=Dendrocoelum lacteum OX=27895 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 6.286e-13
Identity = 50/150 (33.33%), Postives = 78/150 (52.00%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKI 452
            S G Q+P+LL+  SIIPK  CL++G   L +G      R   ++          ++   +  ++ +  R+  F  IY + IS+ I C+ YQ+ NY  W        ++  PI  ++LL +F+ QVVGI SAIW  SLD+  +W  +F  I
Sbjct:  377 SVGGQSPELLMTFSIIPKSICLIVGTLLLCLGFLFLKTRKKTINKN-------GKKIRSITHITSVAFRMGIFSGIYILAISIHIVCELYQYINYNIWRRAPQIFRDIKEPIVAIFLLNIFMGQVVGINSAIWIWSLDAATSWKSVFWNI 519
[Close]
BLAST of Fzd-4-3 vs. TrEMBL
Match: A0A143VP64 (Frizzled-4 OS=Euperipatoides kanangrensis OX=488523 GN=frizzled-4 PE=2 SV=1)

HSP 1 Score: 58.5362 bits (140), Expect = 8.117e-7
Identity = 43/164 (26.22%), Postives = 81/164 (49.39%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQ---QSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPN 491
            G Q+ + L+   + P  + LV+G++FL             L+G V +    NQ      ++  L  + +RI  F  +Y V  +  I C  Y++ N++SW +N P    ++ P   +++LK+F++ VVGI S +W  S  +  +W    ++ TK++      +P+
Sbjct:  395 GNQSFESLMGFVLGPLTAYLVIGISFL-------------LAGFVALFRIRNQMKNDGSKMDKLEVLMVRIGIFSVLYTVPATCVIGCYFYEYTNWSSWTLNIP----ISKPNIEIFMLKIFMSLVVGITSGMWIWSTKTLTSW----QRFTKRFVHRNSNAPS 537
[Close]
BLAST of Fzd-4-3 vs. Ensembl Cavefish
Match: fzd8a (frizzled class receptor 8a [Source:ZFIN;Acc:ZDB-GENE-000328-3])

HSP 1 Score: 45.4394 bits (106), Expect = 9.560e-6
Identity = 37/148 (25.00%), Postives = 62/148 (41.89%), Query Frame = 3 
Query:    9 GEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTL----NLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVEL 440
            G Q  D L    + P +  L +G  FL  G               +I + + Q   +   L  + +RI  F  +Y V  ++ + C  Y+ +N  SW  ++  +      L  P + V++LK F+   VGI S +W  S  +  +W  L
Sbjct:  370 GNQNLDNLRGFVLAPLVIYLFIGTMFLLAGFV----------SLFRIRSVIKQGGTKTDKLEKLMIRIGIFTVLYTVPATIIVACYFYEQHNRQSWETSHNCSCLMEHELRRPDYAVFMLKYFMCLSVGITSGVWIWSGKTLDSWRAL 507
[Close]
BLAST of Fzd-4-3 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002787.1 (pep scaffold:Pmarinus_7.0:GL477229:55:1374:1 gene:ENSPMAG00000002542.1 transcript:ENSPMAT00000002787.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 51.2174 bits (121), Expect = 8.353e-9
Identity = 48/172 (27.91%), Postives = 75/172 (43.60%), Query Frame = 3 
Query:    6 GGEQTPDXXXXXSIIPKISCLVLGLTFLTIGL-AIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLV-------------NNPN-TLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEK 476
            GG  +P+ L    I+P    L+LG +F+  GL A+   R    +G         ++ E+LM      ++I  F  +Y V  +  I C  Y++ N  SW                 P+ T   + P   VYLLK+F++ VVGI S +W  S  +  +W  L+ K   +    K
Sbjct:   25 GGLMSPNALAGFVIVPLTCYLILGTSFILAGLVALFDIRRVMRTGGTD-----TEKLEKLM------VKIGVFSVLYSVPATCVIACYFYEWYNADSWRTLAVASKCRAAQGNQTPDCTAVQSIPSIEVYLLKVFMSLVVGITSGMWIWSSKTLQSWQMLWNKKLSRQTGRK 185
[Close]
BLAST of Fzd-4-3 vs. Ensembl Nematostella
Match: EDO38567 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SCF4])

HSP 1 Score: 47.7506 bits (112), Expect = 5.480e-7
Identity = 34/122 (27.87%), Postives = 57/122 (46.72%), Query Frame = 3 
Query:  144 QILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNP-NT---------LNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKK 479
            +I T L  +      L  + +RI  F  +Y + +S  I C  Y++NN   W V  P NT         L    P   V+++K F+  ++GI S +W  S  +  +W + + K  K  +++KK
Sbjct:  415 RIRTVLKNKETNTDKLEKLIVRIGVFSVLYTIPVSAVIACSFYEYNNMDVWKVRAPFNTYICRGDQACLENYGPSIEVFIVKYFMLLIIGITSNMWIWSTKTFKSWRKFYSK-RKGGEAKKK 535
[Close]
BLAST of Fzd-4-3 vs. Planmine SMEST
Match: SMESG000037981.1 (SMESG000037981.1)

HSP 1 Score: 319.701 bits (818), Expect = 1.875e-108
Identity = 161/164 (98.17%), Postives = 161/164 (98.17%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS 494
            S GEQTPDLLLL SIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIF VYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS
Sbjct:  372 SVGEQTPDLLLLFSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFTVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS 535
[Close]
BLAST of Fzd-4-3 vs. Planmine SMEST
Match: SMESG000037982.1 (SMESG000037982.1)

HSP 1 Score: 107.071 bits (266), Expect = 1.939e-27
Identity = 69/164 (42.07%), Postives = 88/164 (53.66%), Query Frame = 3 
Query:    3 SGGEQTPDXXXXXSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITKKWDSEKKTSPNS 494
            S GEQTPDLLL  SIIP++ CL++G  FL  G      R+ K S K     C +Q+   L+ +     R+  F  IY + I+  ICC+ YQ+ NY  W             I  V+LLK F+NQVVGI SAIW LSLDS  AW + F  + +K    KK   NS
Sbjct:  369 SVGEQTPDLLLTFSIIPQLICLIIGFIFLLSGFFFFKYRTKKFSNKS---LCHHQKITFLLSIG---FRLGLFSIIYIIAIATMICCELYQYYNYHYWSRAPIKFSKRKDAIVAVFLLKFFMNQVVGINSAIWILSLDSVAAWKKCFVMLLEKVTFNKKIKSNS 526
[Close]
BLAST of Fzd-4-3 vs. Planmine SMEST
Match: SMESG000005043.1 (SMESG000005043.1)

HSP 1 Score: 48.521 bits (114), Expect = 5.760e-7
Identity = 37/147 (25.17%), Postives = 63/147 (42.86%), Query Frame = 3 
Query:   45 IIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQILTCLNQQSEQLMD-LSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNN--------PNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKITK 458
            I+P    L++G  FL  G              + I   +  + E   D L  + +RI  F  +Y V  ++ I C  Y+  N   W  +N         +  N + P + V++LK F+  VVGI S  W  +  +  +W   FR++++
Sbjct:  396 IVPLTVYLLIGSCFLLSGFI----------ALISIRKTIRAKGESKTDKLEKLMMRIGIFGLLYSVPAAVVIGCHLYEVKNREFWEKSNNCYCNRNIKSQENFSKPEYSVFMLKYFMCLVVGITSGFWIWTSKTLDSWHNAFRRLSQ 532
[Close]
BLAST of Fzd-4-3 vs. Planmine SMEST
Match: SMESG000014322.1 (SMESG000014322.1)

HSP 1 Score: 47.3654 bits (111), Expect = 1.301e-6
Identity = 33/105 (31.43%), Postives = 54/105 (51.43%), Query Frame = 3 
Query:  189 LSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLVNNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAW-----VEL-FRKITKKWDSEKKTS 485
            L  + +RI  F  ++ V  S  + C+ Y++ N   WL +  N      P   VY+LK+F++ VVGI S +W  S+ +  +W     V++  RK T+    EKK +
Sbjct:  427 LEILMVRIGLFSVLFLVPSSTVLGCNFYEYANRDHWLKSGTN------PNIEVYMLKIFMSSVVGITSGVWVWSIKTLESWQWFCLVKMCCRKPTRSMIEEKKNN 525
The following BLAST results are available for this feature:
BLAST of Fzd-4-3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5
Match NameE-valueIdentityDescription
fz22.058e-624.69gene:FBgn0016797 transcript:FBtr0330195[more]
fz27.967e-624.55gene:FBgn0016797 transcript:FBtr0112890[more]
fz27.967e-624.55gene:FBgn0016797 transcript:FBtr0075029[more]
fz27.967e-624.55gene:FBgn0016797 transcript:FBtr0332735[more]
fz27.967e-624.55gene:FBgn0016797 transcript:FBtr0330194[more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fzd88.603e-625.17frizzled class receptor 8 [Source:NCBI gene;Acc:10... [more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A159X4Q92.888e-2642.07Frizzled-4-3 (Fragment) OS=Schmidtea mediterranea ... [more]
T1DBJ67.772e-2442.07Fzd-4-3 OS=Schmidtea mediterranea OX=79327 PE=2 SV... [more]
T1D1425.224e-1734.01Fzd-4-3a (Fragment) OS=Dendrocoelum lacteum OX=278... [more]
T1E1116.286e-1333.33Fzd-4-3b OS=Dendrocoelum lacteum OX=27895 PE=2 SV=... [more]
A0A143VP648.117e-726.22Frizzled-4 OS=Euperipatoides kanangrensis OX=48852... [more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
fzd8a9.560e-625.00frizzled class receptor 8a [Source:ZFIN;Acc:ZDB-GE... [more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
ENSPMAT00000002787.18.353e-927.91pep scaffold:Pmarinus_7.0:GL477229:55:1374:1 gene:... [more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO385675.480e-727.87Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of Fzd-4-3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of Fzd-4-3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000037981.11.875e-10898.17SMESG000037981.1[more]
SMESG000037982.11.939e-2742.07SMESG000037982.1[more]
SMESG000005043.15.760e-725.17SMESG000005043.1[more]
SMESG000014322.11.301e-631.43SMESG000014322.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011165 ID=SMED30011165|Name=Fzd-4-3|organism=Schmidtea mediterranea sexual|type=transcript|length=496bp
GTTCGGGAGGCGAGCAGACTCCAGATTTACTTTTATTGTTGTCTATTATT
CCAAAAATTAGTTGTTTAGTTTTAGGTTTGACTTTTCTTACAATCGGGCT
TGCAATTCATGCCGGTCGGTCCGATAAATTGAGTGGGAAAGTTCAGATTT
TAACCTGTCTGAATCAACAGAGCGAACAATTGATGGATTTGTCTCCCATG
AAATTAAGGATTAAAACATTTTTGTTTATTTATTGTGTAACAATATCATT
ATCCATTTGCTGTGACGCCTACCAATTTAATAATTACGCGTCTTGGTTGG
TGAATAATCCCAACACCTTAAATTTAACCTTTCCAATCTTTCCAGTTTAC
CTTTTGAAGATGTTTGTGAATCAGGTAGTTGGTATTTATAGTGCGATTTG
GTTCCTTTCTCTTGATTCCCGAGGAGCTTGGGTTGAATTATTTAGAAAAA
TTACCAAAAAATGGGATTCAGAAAAGAAAACTTCTCCCAACTCCCA
back to top

protein sequence of SMED30011165-orf-1

>SMED30011165-orf-1 ID=SMED30011165-orf-1|Name=SMED30011165-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=164bp
SGGEQTPDLLLLLSIIPKISCLVLGLTFLTIGLAIHAGRSDKLSGKVQIL
TCLNQQSEQLMDLSPMKLRIKTFLFIYCVTISLSICCDAYQFNNYASWLV
NNPNTLNLTFPIFPVYLLKMFVNQVVGIYSAIWFLSLDSRGAWVELFRKI
TKKWDSEKKTSPNS
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: INTERPRO
TermDefinition
IPR015526Frizzled/SFRP
IPR000539Frizzled/Smoothened_TM
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0007166cell surface receptor signaling pathway
GO:0007275multicellular organism development
GO:0016055Wnt signaling pathway
Vocabulary: molecular function
TermDefinition
GO:0004888transmembrane signaling receptor activity
GO:0042813Wnt-activated receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 1..153
e-value: 3.2E-17
score: 64.3
NoneNo IPR availablePANTHERPTHR11309:SF99FRIZZLED-3coord: 2..153
NoneNo IPR availableCDDcd139517tmF_Frizzled_SMOcoord: 1..148
e-value: 7.59372E-12
score: 59.64
NoneNo IPR availableTMHMMTMhelixcoord: 15..37
NoneNo IPR availableTMHMMTMhelixcoord: 112..134
NoneNo IPR availableTMHMMTMhelixcoord: 73..92
IPR000539Frizzled/Smoothened, transmembrane domainPFAMPF01534Frizzledcoord: 2..149
e-value: 5.7E-14
score: 51.9
IPR015526Frizzled/secreted frizzled-related proteinPANTHERPTHR11309FRIZZLEDcoord: 2..153