MFS domain-containing protein

Overview
NameMFS domain-containing protein
Smed IDSMED30011135
Length (bp)2013
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of MFS domain-containing protein (SMED30011135) t-SNE clustered cells

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30011135) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of MFS domain-containing protein (SMED30011135) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for MFS domain-containing protein (SMED30011135) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011135

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 12

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
pigment cellSMED30011135h1SMcG0004953 SmedASXL_078659SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30011135h1SMcG0004953 SmedASXL_070184SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
pigment cellSMED30011135h1SMcG0004953 SmedASXL_069607SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017		whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30011135h1SMcG0004953 Contig48725newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011135h1SMcG0004953 Contig48725uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011135h1SMcG0004953 Contig13260uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011135h1SMcG0004953 Contig13260newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
gutSMED30011135h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011135h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011135h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30011135h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30011135h1SMcG0004953 dd_Smed_v4_3455_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 88.5817 bits (218), Expect = 2.368e-18
Identity = 61/213 (28.64%), Postives = 114/213 (53.52%), Query Frame = 2 
Query: 1136 VQEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILT 1774
            V+ E L+   S I   +          V   +R   F +L+ +   N V +   S LYK++GQ FI +D F +++G+I+++FN+G R+ WG + DT SY+  MS +  L +++     FS  +  ++   +++   VCL+F C+ G+F++ P  T  CF   N ++ YG +    +++ ++  L++++ L  I +   F++  C  + SL LT
Sbjct:  240 VETENLIGTNSEIRVEQA--------RVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLT 440
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693414:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 88.1965 bits (217), Expect = 2.956e-18
Identity = 61/213 (28.64%), Postives = 114/213 (53.52%), Query Frame = 2 
Query: 1136 VQEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILT 1774
            V+ E L+   S I   +          V   +R   F +L+ +   N V +   S LYK++GQ FI +D F +++G+I+++FN+G R+ WG + DT SY+  MS +  L +++     FS  +  ++   +++   VCL+F C+ G+F++ P  T  CF   N ++ YG +    +++ ++  L++++ L  I +   F++  C  + SL LT
Sbjct:  226 VETENLIGTNSEIRVEQA--------RVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLT 426
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: F10G7.5 (pep chromosome:WBcel235:II:4693542:4696408:1 gene:WBGene00017369.1 transcript:F10G7.5a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:F10G7.5)

HSP 1 Score: 88.1965 bits (217), Expect = 2.956e-18
Identity = 61/213 (28.64%), Postives = 114/213 (53.52%), Query Frame = 2 
Query: 1136 VQEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILT 1774
            V+ E L+   S I   +          V   +R   F +L+ +   N V +   S LYK++GQ FI +D F +++G+I+++FN+G R+ WG + DT SY+  MS +  L +++     FS  +  ++   +++   VCL+F C+ G+F++ P  T  CF   N ++ YG +    +++ ++  L++++ L  I +   F++  C  + SL LT
Sbjct:  226 VETENLIGTNSEIRVEQA--------RVWPQLRTTTFLVLFLSLTCNAVWVQLTSGLYKAYGQQFIDSDFFLSLIGSISSIFNAGSRVFWGAIADTTSYQFSMSIVCSLGAVLA----FSMPLVKMVSNDVLFLATVCLMFSCIGGTFSIFPYITHKCFSKANFSVMYGFLQCAVSVAGLIAGLISTHALSEIGFEYLFIVCGCFMLTSLFLT 426
[Close]
BLAST of MFS domain-containing protein vs. Ensembl Celegans
Match: Y59A8B.21 (pep chromosome:WBcel235:V:17986260:17994546:1 gene:WBGene00013353.1 transcript:Y59A8B.21.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y59A8B.21)

HSP 1 Score: 62.003 bits (149), Expect = 6.837e-10
Identity = 48/166 (28.92%), Postives = 86/166 (51.81%), Query Frame = 2 
Query: 1223 ESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEY 1720
            E ++   F+ L+++ F          +LYK++G++FI +D F A+  +IA+V N+  R+ WG L D  S++  +ST T L S+ + T     M  +  +G++ +  ++   F C+  + A+    T  CFG+ + A  YG +      S IL A ++   L+ I Y
Sbjct:  269 EMLKSPTFYCLFASLFCCSFYANMFYNLYKTYGESFIEDDMFMAMAFSIASVANAIARIGWGYLTDRTSFQIALSTATCLASVFLLT-----MPMTRELGKMAFLFWLVGTFICMGATHALFITATVKCFGNRHKANNYGYLILSTTTSGILLAAISQFYLKAIGY 429
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|Q51330|OXLT_OXAFO (Oxalate:formate antiporter OS=Oxalobacter formigenes OX=847 GN=oxlT PE=1 SV=3)

HSP 1 Score: 67.0106 bits (162), Expect = 2.272e-10
Identity = 53/197 (26.90%), Postives = 93/197 (47.21%), Query Frame = 2 
Query: 1199 TGRDYEVMESVRECRFWILWSTFF---FNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQF 1780
            T +D+   E +R  +FW+LW+ FF   F G+++V  S  Y   G++         I  +I  +FN G R  WG + D     + MS +  + ++V+  F     I +  +G + +   + + FF   GS+A+ P+T S  FG+   A  YG  +   A +SI G  + + +     +   FL+   ++ I+  L  F
Sbjct:  207 TDKDFNSGEMLRTPQFWVLWTAFFSVNFGGLLLVANSVPY---GRSLGLAAGVLTIGVSIQNLFNGGCRPFWGFVSDKIGRYKTMSVVFGINAVVLALF---PTIAA--LGDVAFIAMLAIAFFTWGGSYALFPSTNSDIFGTAYSARNYGFFWAAKATASIFGGGLGAAIATNFGWNTAFLITAITSFIAFALATF 395
[Close]
BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Match: sp|P37662|YHJX_ECOLI (Uncharacterized MFS-type transporter YhjX OS=Escherichia coli (strain K12) OX=83333 GN=yhjX PE=1 SV=1)

HSP 1 Score: 56.9954 bits (136), Expect = 3.505e-7
Identity = 41/181 (22.65%), Postives = 85/181 (46.96%), Query Frame = 2 
Query: 1175 EENELSVPTG---RDYEVMESVRECRFWILWSTFF---FNGVVIVTISSLYKSFGQTFISNDKXXXX-XXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTS 1696
             + E+    G   +DY + ES+R+ ++W+L   F     +G+ ++ ++   K   Q+    D   A     + ++ N  GRL+ G L D  +  R+++   ++  + M    F+ +        + +   +  + F   G+  + P+  S  FG  NLA  YG+++ G+ + SI G+++ S
Sbjct:  190 PKQEVKTSNGVVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGVA---KDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGMAALLFAPL------NAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIGSICGSIIAS 361
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A0K2SYD6 (Oxalate:formate antiporterlike [Strongylocentrotus purpuratus] OS=Lepeophtheirus salmonis OX=72036 PE=4 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 5.450e-33
Identity = 84/189 (44.44%), Postives = 121/189 (64.02%), Query Frame = 2 
Query: 1223 ESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 1789
            + +R   FWILW+TF  N   I  I+S+YK+FGQTFI +D F AIVGA AA+FNSGGR+ WG L D F YR  +  ++ L+SI+ GTFY  S+ KS    + ++A +V  +FFC  G+F ++P  T+ CFG+  L+  YG+V +G A+ +++ A VT  L   I + G FLL +    +S I+  FFP+
Sbjct:  273 QVIRSIEFWILWTTFLLNSQSIGYINSMYKAFGQTFIKDDHFLAIVGAFAAIFNSGGRIFWGHLCDIFGYRICIMVVSSLLSILYGTFYAISLTKS----KALFASWVWAIFFCFCGNFVLMPTATAQCFGTKFLSKNYGLVMSGMALGALVLAAVTQVLQPFIGFLGMFLLMSAVCTLSCIINSFFPS 457
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: W4XBI5 (MFS domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 3.617e-32
Identity = 85/205 (41.46%), Postives = 127/205 (61.95%), Query Frame = 2 
Query: 1169 SIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFF 1783
             ++  + +  +G D      VR   FW+LW+TF  NG  I+ ISSLYK++GQ FI +D F A+VG+ AAVFN+GGRL WG L D FSY+  M  LT   +I     +  +++ +   G+ ++ +++C +FF  +G+FA+ P  T+  FGS  +   YGIVFT  A+SS LGA++ S L E + ++G F+L    +II L+LT FF
Sbjct:  277 PVDNGKDTKASGLDLPPKVMVRTTAFWLLWATFLVNGQGIMFISSLYKAYGQMFIQDDHFLALVGSFAAVFNAGGRLFWGWLADRFSYKLAMILLTGSNAI-----FLLTLVAAQYGGRAMFFVWICGIFFTFSGNFALFPTATARAFGSKYIGPNYGIVFTSVALSSALGAVLVSTLHEHLSWFGMFMLMGGFSIIGLVLTFFF 476
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1S3HHM8 (uncharacterized protein LOC106155360 isoform X2 OS=Lingula unguis OX=7574 GN=LOC106155360 PE=4 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 5.510e-32
Identity = 86/219 (39.27%), Postives = 131/219 (59.82%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEE----NELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFF 1783
             E  LL E  S  +     +++    RDY   + +R   F+ILW  F  NG  ++  SSLYK++GQTFI +D+F AIVGA AAVFN+GGR+ WG + D FS++  M  L   M  +M TF     I + L G++++ I+VCL+F   +G+F++ P  T+  FG+ ++A+ YG++FT   ++  LGA +T  LL +I ++  F +    + ISLILT  F
Sbjct:  111 DESALLPEDRSDSDVDYKKKMAPNIARDYYPKQMIRSKYFYILWFIFLLNGQAVIFSSSLYKAYGQTFIKDDQFLAIVGAFAAVFNAGGRIFWGVIADKFSFKTAMLCLCTSMCALMMTF-----IIAPLGGKVMFFIWVCLIFGTFSGNFSLFPTATAKAFGAKHVAVNYGLLFTSQVIAPPLGAFLTQQLLHLIGWFFMFCIVAGCSFISLILTFLF 324
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A1S3HKS6 (uncharacterized protein LOC106155360 isoform X1 OS=Lingula unguis OX=7574 GN=LOC106155360 PE=4 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 7.827e-31
Identity = 86/219 (39.27%), Postives = 131/219 (59.82%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEE----NELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFF 1783
             E  LL E  S  +     +++    RDY   + +R   F+ILW  F  NG  ++  SSLYK++GQTFI +D+F AIVGA AAVFN+GGR+ WG + D FS++  M  L   M  +M TF     I + L G++++ I+VCL+F   +G+F++ P  T+  FG+ ++A+ YG++FT   ++  LGA +T  LL +I ++  F +    + ISLILT  F
Sbjct:  243 DESALLPEDRSDSDVDYKKKMAPNIARDYYPKQMIRSKYFYILWFIFLLNGQAVIFSSSLYKAYGQTFIKDDQFLAIVGAFAAVFNAGGRIFWGVIADKFSFKTAMLCLCTSMCALMMTF-----IIAPLGGKVMFFIWVCLIFGTFSGNFSLFPTATAKAFGAKHVAVNYGLLFTSQVIAPPLGAFLTQQLLHLIGWFFMFCIVAGCSFISLILTFLF 456
[Close]
BLAST of MFS domain-containing protein vs. TrEMBL
Match: A0A0H3YF06 (Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=slc16a-5 PE=2 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.540e-30
Identity = 79/211 (37.44%), Postives = 129/211 (61.14%), Query Frame = 2 
Query: 1148 KLLDETSSIEEN--ELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILT 1774
              + ET   E N   L      D  + +++++  FWILW  F F G + V  +SLYK+FGQ FI++D + A+VG++A++FN+ GR+ WG+++D FSY+  + ++ I  +I+MGTFY   ++ + +  ++++ I+VC +F  + G FA++PA T+  FG   +   YGI+F+  A + I GAL TS  L  I Y+G F +      I+LILT
Sbjct:  245 NFISETYDDENNIQSLLPKDNHDSNIFKAIKQRDFWILWFIFMFCGTINVYATSLYKTFGQLFITSDYYLALVGSVASIFNAMGRIFWGQVMDRFSYKVAIRSIIINSTILMGTFYLCHLVPNSIGARVMFLIWVCGIFANICGIFAVMPAITAKLFGLKKVVTIYGILFSANAFAGIFGALTTSIFLSKINYFGIFGIGAGLTTIALILT 455
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 406.757 bits (1044), Expect = 5.808e-137
Identity = 216/217 (99.54%), Postives = 216/217 (99.54%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 1789
             EEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT
Sbjct:  158 DEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 374
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 406.757 bits (1044), Expect = 9.072e-137
Identity = 216/217 (99.54%), Postives = 216/217 (99.54%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 1789
             EEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT
Sbjct:  173 DEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 389
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060691.1 (SMESG000060691.1)

HSP 1 Score: 406.757 bits (1044), Expect = 2.194e-136
Identity = 216/217 (99.54%), Postives = 216/217 (99.54%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 1789
             EEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT
Sbjct:  195 DEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFPT 411
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1)

HSP 1 Score: 246.128 bits (627), Expect = 6.856e-74
Identity = 131/217 (60.37%), Postives = 171/217 (78.80%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEENELSVPTG-RDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFP 1786
             E K L E   I++ +L       D  V E++++ R WIL STFF NG+VI+ ISSLYKSFGQTFISND++F+IVGA+AA+FNSGGRL+WGRLLD  SYR++M+ LTI MS++MG+FYF  ++K  +   I+YAI+VCLLFFCL+GSFAM PA TS CFG+TN+AI YGIVF G+A+S ++GAL+ S+LL +I Y G F++AT S+IISL+LTQFFP
Sbjct:  240 NEAKGLKELPEIKQAKLDPKVLLTDSSVKEAIKDKRLWILCSTFFLNGIVIIIISSLYKSFGQTFISNDRYFSIVGAVAAIFNSGGRLVWGRLLDKISYRKMMTILTISMSVMMGSFYFCGLVKHEMGVLIMYAIWVCLLFFCLSGSFAMFPAITSQCFGTTNMAIIYGIVFLGFAVSGVIGALICSSLLSLINYGGLFIVATLSSIISLVLTQFFP 456
[Close]
BLAST of MFS domain-containing protein vs. Planmine SMEST
Match: SMESG000060699.1 (SMESG000060699.1)

HSP 1 Score: 246.514 bits (628), Expect = 7.926e-74
Identity = 131/217 (60.37%), Postives = 171/217 (78.80%), Query Frame = 2 
Query: 1139 QEEKLLDETSSIEENELSVPTG-RDYEVMESVRECRFWILWSTFFFNGVVIVTISSLYKSFGQTFISNDKXXXXXXXXXXXXNSGGRLIWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVCLLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSNLLEVIEYWGQFLLATCSAIISLILTQFFP 1786
             E K L E   I++ +L       D  V E++++ R WIL STFF NG+VI+ ISSLYKSFGQTFISND++F+IVGA+AA+FNSGGRL+WGRLLD  SYR++M+ LTI MS++MG+FYF  ++K  +   I+YAI+VCLLFFCL+GSFAM PA TS CFG+TN+AI YGIVF G+A+S ++GAL+ S+LL +I Y G F++AT S+IISL+LTQFFP
Sbjct:  254 NEAKGLKELPEIKQAKLDPKVLLTDSSVKEAIKDKRLWILCSTFFLNGIVIIIISSLYKSFGQTFISNDRYFSIVGAVAAIFNSGGRLVWGRLLDKISYRKMMTILTISMSVMMGSFYFCGLVKHEMGVLIMYAIWVCLLFFCLSGSFAMFPAITSQCFGTTNMAIIYGIVFLGFAVSGVIGALICSSLLSLINYGGLFIVATLSSIISLVLTQFFP 470
The following BLAST results are available for this feature:
BLAST of MFS domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 4
Match NameE-valueIdentityDescription
F10G7.52.368e-1828.64pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.52.956e-1828.64pep chromosome:WBcel235:II:4693414:4696408:1 gene:... [more]
F10G7.52.956e-1828.64pep chromosome:WBcel235:II:4693542:4696408:1 gene:... [more]
Y59A8B.216.837e-1028.92pep chromosome:WBcel235:V:17986260:17994546:1 gene... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q51330|OXLT_OXAFO2.272e-1026.90Oxalate:formate antiporter OS=Oxalobacter formigen... [more]
sp|P37662|YHJX_ECOLI3.505e-722.65Uncharacterized MFS-type transporter YhjX OS=Esche... [more]
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BLAST of MFS domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A0K2SYD65.450e-3344.44Oxalate:formate antiporterlike [Strongylocentrotus... [more]
W4XBI53.617e-3241.46MFS domain-containing protein OS=Strongylocentrotu... [more]
A0A1S3HHM85.510e-3239.27uncharacterized protein LOC106155360 isoform X2 OS... [more]
A0A1S3HKS67.827e-3139.27uncharacterized protein LOC106155360 isoform X1 OS... [more]
A0A0H3YF061.540e-3037.44Slc16a-5 (Fragment) OS=Schmidtea mediterranea OX=7... [more]
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BLAST of MFS domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of MFS domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000060691.15.808e-13799.54SMESG000060691.1[more]
SMESG000060691.19.072e-13799.54SMESG000060691.1[more]
SMESG000060691.12.194e-13699.54SMESG000060691.1[more]
SMESG000060699.16.856e-7460.37SMESG000060699.1[more]
SMESG000060699.17.926e-7460.37SMESG000060699.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011135 ID=SMED30011135|Name=MFS domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=2013bp
CATATTAATCACCATGTGAAAGTGGAATATTTAATGAAAAAAAAACTTTT
TCATTTTTTAAATTAAGGTAGTAAATTAAGTTTGTCCATAAAAATATCGA
TATAGAATTTTATGGTATGTCAACAACTATTTGTTATGTGACGTTTTTCA
AGTATTTACTAGATTGAATCATGTTAAATATCTTTTCAGCTATACAAAAT
AACACTTTTACATTTCACTAGGTGATTAATATGCTAAATAATAATCCTTA
TAATGGTCTATCTGTAAATCTATCGAAAAGATGATGGCCCATTTTTAATA
AAAACACGAGAAAGCCAAATAAACGACATATATTTCGAGGAAATTATCTT
CATCATCAGTCTTCCTTCATAATTTTTGTCAAATGCTATGTATGGAAAGA
ATAATAATGTAATAGTTATGACTTAATTAATTGCTGATTTAATCAGTGAT
TTAATCGCCAATGCTTCACAAAAATTCTTTGCACATTTTTCTATTCTCAA
TAATATTGCTCACGTCTCCCAGTCGGCTATGTTCCTAAACTCTTAAACTT
TTTATTTGGGATGGAAATTATAAGTGTTTTGCGTAAATTATCCATCTTAA
CGAATTGTGGATCTTCAAGTATCTTATACAATTTTTCAAAATAAACTTAA
TTCAAAACTACAAATCCGTTTCTTTTATCAAATTTACAAACATTCGCTGG
TTTTTAGAAAGTTTCTTTAAAACATGATTTATTTTTCCGAACTGCATTTG
ATGTAAGCTGTGTTTAACTTTATCTATAATGTAGATTCTACATTAATAAA
TTGGTTAATTGAAAAATTTCTCGAATAACCAAAATATGATTCAATCTTGG
CCATAAACCGTTGGTCATCAATACGTTTAGGTCCATATATCAAAACATTA
ATTCTTTCAGAATTAAGATCTATGTCATTACAATTAACAAGTGCATGTTT
AATATTAACATTAAAGTTAAAAACACAAAACATCTTTCTTAATTGAATTA
ATTTTATCAATCAGCAATTTAAAGATACAATAATAGCCATTTTAATACAT
AAATCAAATCATTCATTACCAATTACCCCATACAACATTAACCGGAAAAA
TGCATATTTATTTATTTATGATTAATATTTGTTTTGTTCAGGAAGAAAAG
TTACTGGACGAAACTTCAAGTATTGAAGAAAATGAGCTTTCTGTTCCAAC
TGGAAGAGATTATGAAGTAATGGAATCAGTTCGTGAATGTCGTTTCTGGA
TTTTATGGAGCACATTTTTCTTCAATGGGGTGGTTATTGTCACGATATCG
TCACTATATAAAAGTTTCGGTCAAACTTTCATTAGCAATGATAAGTTCTT
CGCGATTGTTGGAGCTATAGCGGCGGTTTTTAACTCCGGCGGACGATTAA
TTTGGGGGCGATTATTAGACACTTTTTCATACAGGAGGATTATGTCGACC
CTCACCATCCTGATGTCAATTGTGATGGGAACATTTTATTTTTCAAGCAT
GATCAAATCAGTCCTTATCGGTCAAATAGTATACGCAATTTATGTTTGTT
TGCTATTCTTTTGCTTGGCTGGTAGTTTTGCAATGCTTCCGGCTACAACA
TCACACTGTTTTGGTAGCACAAACCTAGCAATAACTTATGGAATCGTGTT
TACTGGCTATGCTATGTCGAGTATTTTAGGAGCTTTGGTTACAAGCAATC
TTTTAGAAGTCATTGAATATTGGGGGCAATTTCTCCTCGCAACATGCTCG
GCAATAATTAGTCTGATTCTGACGCAATTTTTCCCCACAAAGTCCTCCCG
TTTGAGCAAGTAATTGTCTTTGGAAGTGTCAGATCGAGAGCATTTATTCA
CTCGATGTTTAGAATTAAATATTATTTGTTGTAGGATATATTGAATTCCT
GACATTTTAGGTTCGGTTTTAGCATTAATTTCGTTGATGATTATTTCAAG
AGCTTTCATTAGTATTTATATTTCATATGGTGGTTAATAAATAGAGGACC
TTGAATAGTTCAT
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protein sequence of SMED30011135-orf-1

>SMED30011135-orf-1 ID=SMED30011135-orf-1|Name=SMED30011135-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=101bp
MKALEIIINEINAKTEPKMSGIQYILQQIIFNSKHRVNKCSRSDTSKDNY
LLKREDFVGKNCVRIRLIIAEHVARRNCPQYSMTSKRLLVTKAPKILDIA
*
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protein sequence of SMED30011135-orf-2

>SMED30011135-orf-2 ID=SMED30011135-orf-2|Name=SMED30011135-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=238bp
MHIYLFMINICFVQEEKLLDETSSIEENELSVPTGRDYEVMESVRECRFW
ILWSTFFFNGVVIVTISSLYKSFGQTFISNDKFFAIVGAIAAVFNSGGRL
IWGRLLDTFSYRRIMSTLTILMSIVMGTFYFSSMIKSVLIGQIVYAIYVC
LLFFCLAGSFAMLPATTSHCFGSTNLAITYGIVFTGYAMSSILGALVTSN
LLEVIEYWGQFLLATCSAIISLILTQFFPTKSSRLSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: molecular function
TermDefinition
GO:0022857transmembrane transporter activity
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR036259MFS_trans_sf
IPR020846MFS_dom
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: biological process
TermDefinition
GO:0055085transmembrane transport
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:1.20.1250.20coord: 3..234
e-value: 7.0E-17
score: 63.0
NoneNo IPR availablePANTHERPTHR11360:SF3OXALATE/FORMATE ANTIPORTERcoord: 23..232
NoneNo IPR availablePANTHERPTHR11360MONOCARBOXYLATE TRANSPORTERcoord: 23..232
NoneNo IPR availableCDDcd17353MFS_OFA_likecoord: 28..228
e-value: 1.0309E-35
score: 128.112
NoneNo IPR availableTMHMMTMhelixcoord: 208..230
NoneNo IPR availableTMHMMTMhelixcoord: 176..198
NoneNo IPR availableTMHMMTMhelixcoord: 49..68
NoneNo IPR availableTMHMMTMhelixcoord: 83..102
NoneNo IPR availableTMHMMTMhelixcoord: 141..163
NoneNo IPR availableTMHMMTMhelixcoord: 109..131
IPR036259MFS transporter superfamilySUPERFAMILYSSF103473MFS general substrate transportercoord: 42..233