SMED30011134

Overview
NameSMED30011134
Smed IDSMED30011134
Length (bp)2107
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011134 (SMED30011134) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011134 (SMED30011134) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011134 (SMED30011134) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011134 (SMED30011134) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011134

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30011134SMESG000058633.1 Contig2529newmark_estsPMID:23079596
Forsthoefel et al., 2012
FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
headSMED30011134SMESG000063657.1 SmedASXL_018433SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
headSMED30011134SMESG000063657.1 SmedASXL_018432SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
pharynxSMED30011134SMESG000063657.1 dd_Smed_v4_10132_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30011134SMESG000063657.1 dd_Smed_v4_10132_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30011134SMESG000063657.1 dd_Smed_v4_10132_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
neuronSMED30011134SMESG000063657.1 dd_Smed_v4_10132_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult colorimetric in situ hybridization evidence
headSMED30011134SMESG000063657.1 dd_Smed_v6_10132_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
headSMED30011134 dd_Smed_v6_28080_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
epidermisSMED30011134SMESG000063657.1 dd_Smed_v4_10132_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011134 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003435:16143..20517 -10258
Homology
BLAST of SMED30011134 vs. Ensembl Xenopus
Match: ENSXETT00000017042.1 (amyotrophic lateral sclerosis 2 chromosome region 12 [Source:NCBI gene;Acc:100494057])

HSP 1 Score: 53.5286 bits (127), Expect = 8.512e-7
Identity = 57/207 (27.54%), Postives = 98/207 (47.34%), Query Frame = 2
Query: 1139 FQASFDSYRQALI---------KESENK--IEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRR-------ELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNER 1705
             + SF +Y+++L          KE++ K   E +K R L   N++L D  +K+KKEL    E +++ +++ H  ++ ++   +K                         EV   K+++QKL    ENL    ++ +R       E      ENE LKS++    ++   RV +V+  +KQRI SL N+  ++RR  + K E+L NER
Sbjct:  274 LRGSFSTYKESLCEEINESWAQKEAQWKEAFEEKKVRALSKQNQELRDLFEKEKKELWRQAEEELDMVQESHKKQVNEVWEKYK-------------------------EV---KQEFQKLNIMKENLQAEIAENKRIISSQHLEAQKVHRENEMLKSQVDHLRKQTHVRVSKVEAQFKQRIVSLTNENADLRRRLITKSEQLFNER 452          
BLAST of SMED30011134 vs. Ensembl Xenopus
Match: ENSXETT00000017018.1 (amyotrophic lateral sclerosis 2 chromosome region 12 [Source:NCBI gene;Acc:100494057])

HSP 1 Score: 53.5286 bits (127), Expect = 9.327e-7
Identity = 57/207 (27.54%), Postives = 98/207 (47.34%), Query Frame = 2
Query: 1139 FQASFDSYRQALI---------KESENK--IEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRR-------ELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNER 1705
             + SF +Y+++L          KE++ K   E +K R L   N++L D  +K+KKEL    E +++ +++ H  ++ ++   +K                         EV   K+++QKL    ENL    ++ +R       E      ENE LKS++    ++   RV +V+  +KQRI SL N+  ++RR  + K E+L NER
Sbjct:  252 LRGSFSTYKESLCEEINESWAQKEAQWKEAFEEKKVRALSKQNQELRDLFEKEKKELWRQAEEELDMVQESHKKQVNEVWEKYK-------------------------EV---KQEFQKLNIMKENLQAEIAENKRIISSQHLEAQKVHRENEMLKSQVDHLRKQTHVRVSKVEAQFKQRIVSLTNENADLRRRLITKSEQLFNER 430          
BLAST of SMED30011134 vs. Ensembl Xenopus
Match: ENSXETT00000031791.1 (amyotrophic lateral sclerosis 2 chromosome region 12 [Source:NCBI gene;Acc:100494057])

HSP 1 Score: 53.1434 bits (126), Expect = 1.137e-6
Identity = 57/207 (27.54%), Postives = 98/207 (47.34%), Query Frame = 2
Query: 1139 FQASFDSYRQALI---------KESENK--IEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRR-------ELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNER 1705
             + SF +Y+++L          KE++ K   E +K R L   N++L D  +K+KKEL    E +++ +++ H  ++ ++   +K                         EV   K+++QKL    ENL    ++ +R       E      ENE LKS++    ++   RV +V+  +KQRI SL N+  ++RR  + K E+L NER
Sbjct:  251 LRGSFSTYKESLCEEINESWAQKEAQWKEAFEEKKVRALSKQNQELRDLFEKEKKELWRQAEEELDMVQESHKKQVNEVWEKYK-------------------------EV---KQEFQKLNIMKENLQAEIAENKRIISSQHLEAQKVHRENEMLKSQVDHLRKQTHVRVSKVEAQFKQRIVSLTNENADLRRRLITKSEQLFNER 429          
BLAST of SMED30011134 vs. TrEMBL
Match: A0A2T7PZY1 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_01608 PE=4 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 1.210e-21
Identity = 75/277 (27.08%), Postives = 144/277 (51.99%), Query Frame = 2
Query: 1127 ELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRLQNDSHKVPADTNNSDVFIRP 1957
            E++  Q +FDSY+ +L +E +++ + ++E L   C       + + K +L     V+  +  +EH+ ++E L+  HK E+D   RRF+ A A  ++L KT  E+E+ + + Q +Q +Y    Q  +   R LT       + K  + +F ERF E++  +DD Y+ ++  L +Q  E+RR Y+ KC++L +E+  ++++   +++SAK+ ++ +I T+ +A IS+     D  ++   K R          SS    + ++ K       +D   RP
Sbjct:  214 EIEFLQGAFDSYKSSLHQEMDDRWKKKEEELRVQCEEQTQTAIQEMKTKLVQERNVERATSAKEHNRQMESLRKEHKKELDAFVRRFSGAAADMERLKKTTAELEETQAELQHIQEQYNETCQQLANTTRNLT-------DAKVRLMDFEERFQEKIGEIDDKYRLKLQDLMSQNTELRRMYVQKCDQLFDEKTMSEQRTMQEVQSAKEALELLIKTKHKANISIAFSAEDLNSQRTPKVRP---------SSAPGTRCETKKAHLSAGETDHLFRP 474          
BLAST of SMED30011134 vs. TrEMBL
Match: A0A1I8II15 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 5.750e-20
Identity = 61/229 (26.64%), Postives = 126/229 (55.02%), Query Frame = 2
Query: 1139 FQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSE 1825
             ++SFD+++ +  K+ + K   ++  L+          +D+   EL    E ++E++K  H+ E++KL+  H+ E+  L+ R++D      +L+   +++  ++   + L  + E+     + + R L        N +SE+  F  R DERV+ VD+ YK R+ +++ + V++R+AY+ KCE+LV  RK  D +L  ++  +K++M+++I  R R  +S+ +L+   E
Sbjct:  230 LKSSFDTFKDSYTKDLDEKWRKKEAELIAEGEARAKRLVDEATSELLKEREAEVEALKTSHNSELQKLQLEHEKELSELHNRYSDMEGTVQRLNSATRDLVAKEEKVKSLTSQLESRENEVASLERNLA-------NTRSELAAFKARLDERVRLVDEKYKARMATMRLEAVDLRKAYITKCEQLVESRKTEDTKLGERVYKSKEIMRDVIKARHRVEVSMAALDPSEE 451          
BLAST of SMED30011134 vs. TrEMBL
Match: A0A267EK79 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig023567g1 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 6.818e-20
Identity = 61/229 (26.64%), Postives = 126/229 (55.02%), Query Frame = 2
Query: 1139 FQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSE 1825
             ++SFD+++ +  K+ + K   ++  L+          +D+   EL    E ++E++K  H+ E++KL+  H+ E+  L+ R++D      +L+   +++  ++   + L  + E+     + + R L        N +SE+  F  R DERV+ VD+ YK R+ +++ + V++R+AY+ KCE+LV  RK  D +L  ++  +K++M+++I  R R  +S+ +L+   E
Sbjct:  257 LKSSFDTFKDSYTKDLDEKWRKKEAELIAEGEARAKRLVDEATSELLKEREAEVEALKTSHNSELQKLQLEHEKELSELHNRYSDMEGTVQRLNSATRDLVAKEEKVKSLTSQLESRENEVASLERNLA-------NTRSELAAFKARLDERVRLVDEKYKARMATMRLEAVDLRKAYITKCEQLVESRKTEDTKLGERVYKSKEIMRDVIKARHRVEVSMAALDPSEE 478          
BLAST of SMED30011134 vs. TrEMBL
Match: A0A267FG36 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig025973g2 PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.896e-19
Identity = 60/229 (26.20%), Postives = 125/229 (54.59%), Query Frame = 2
Query: 1139 FQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSE 1825
             ++SFD+++ +  K+ + K   ++  L+          +D+   EL    E ++E++K  H+ E++K +  H+ E+  L+ R++D      +L+   +++  ++   + L  + E+     + + R L        N +SE+  F  R DERV+ VD+ YK R+ +++ + V++R+AY+ KCE+LV  RK  D +L  ++  +K++M+++I  R R  +S+ +L+   E
Sbjct:  257 LKSSFDTFKDSYTKDLDEKWRKKEAELIAEGEARAKRLVDEATSELLKEREAEVEALKTSHNTELQKFQLEHEKELSELHNRYSDMEGTVQRLNSATRDLVAKEEKVKSLTSQLESRENEVASLERNLA-------NTRSELAAFKARLDERVRLVDEKYKARMATMRLEAVDLRKAYITKCEQLVESRKTEDTKLGERVYKSKEIMRDVIKARHRVEVSMAALDPSEE 478          
BLAST of SMED30011134 vs. TrEMBL
Match: A0A1I8I9Y4 (Uncharacterized protein OS=Macrostomum lignano OX=282301 PE=4 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.012e-19
Identity = 60/229 (26.20%), Postives = 125/229 (54.59%), Query Frame = 2
Query: 1139 FQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSE 1825
             ++SFD+++ +  K+ + K   ++  L+          +D+   EL    E ++E++K  H+ E++K +  H+ E+  L+ R++D      +L+   +++  ++   + L  + E+     + + R L        N +SE+  F  R DERV+ VD+ YK R+ +++ + V++R+AY+ KCE+LV  RK  D +L  ++  +K++M+++I  R R  +S+ +L+   E
Sbjct:  148 LKSSFDTFKDSYTKDLDEKWRKKEAELIAEGEARAKRLVDEATSELLKEREAEVEALKTSHNTELQKFQLEHEKELSELHNRYSDMEGTVQRLNSATRDLVAKEEKVKSLTSQLESRENEVASLERNLA-------NTRSELAAFKARLDERVRLVDEKYKARMATMRLEAVDLRKAYITKCEQLVESRKTEDTKLGERVYKSKEIMRDVIKARHRVEVSMAALDPSEE 369          
BLAST of SMED30011134 vs. Ensembl Nematostella
Match: EDO45787 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RRM6])

HSP 1 Score: 65.4698 bits (158), Expect = 3.685e-11
Identity = 46/148 (31.08%), Postives = 77/148 (52.03%), Query Frame = 2
Query: 1253 LDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELV 1696
            L +Q+KEL      +++ + +EH  +IE +   H  EID L  +FA+ V   + L K L E++   ++   L+   E L     K+R+       E E+ K ++  + E F E+V  VD+ Y  RI  L +   ++RR Y+ KCEE++
Sbjct:  289 LGQQRKELIHEKNKELDGLNKEHQRQIETIVEQHGKEIDRLMEKFAECVQDSENLKKVLTEMQVMTKENASLK---EKLKSTSDKLRKTTV----ELEDKKVKLAGYEEHFAEKVAEVDNKYSNRIHGLMSDNTDLRRHYVKKCEEVI 429          
BLAST of SMED30011134 vs. Planmine SMEST
Match: SMESG000063657.1 (SMESG000063657.1)

HSP 1 Score: 619.002 bits (1595), Expect = 0.000e+0
Identity = 305/307 (99.35%), Postives = 307/307 (100.00%), Query Frame = 2
Query: 1112 DKSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRLQNDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL 2032
            +KSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRL+NDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL
Sbjct:  251 NKSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRLRNDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL 557          
BLAST of SMED30011134 vs. Planmine SMEST
Match: SMESG000063657.1 (SMESG000063657.1)

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 304/312 (97.44%), Postives = 307/312 (98.40%), Query Frame = 2
Query: 1112 DKSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQ-----KKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRLQNDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL 2032
            +KSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQ     +KELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRL+NDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL
Sbjct:  251 NKSQQELQTFQASFDSYRQALIKESENKIEMEKERLLDCCNRDLSDKLDKQNLYLIQKELKCNFEVDIESIKQEHSCEIEKLKSNHKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAYSKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQIVEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISLVSLESDSETEEFWKTRNKTEEAKSFLSSFGRLRNDSHKVPADTNNSDVFIRPISAPTTEMDEKNVKMQSFLLNKDIL 562          
The following BLAST results are available for this feature:
BLAST of SMED30011134 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
ENSXETT00000017042.18.512e-727.54amyotrophic lateral sclerosis 2 chromosome region ... [more]
ENSXETT00000017018.19.327e-727.54amyotrophic lateral sclerosis 2 chromosome region ... [more]
ENSXETT00000031791.11.137e-627.54amyotrophic lateral sclerosis 2 chromosome region ... [more]
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BLAST of SMED30011134 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2T7PZY11.210e-2127.08Uncharacterized protein OS=Pomacea canaliculata OX... [more]
A0A1I8II155.750e-2026.64Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267EK796.818e-2026.64Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267FG361.896e-1926.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8I9Y42.012e-1926.20Uncharacterized protein OS=Macrostomum lignano OX=... [more]
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BLAST of SMED30011134 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO457873.685e-1131.08Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30011134 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011134 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000063657.10.000e+099.35SMESG000063657.1[more]
SMESG000063657.10.000e+097.44SMESG000063657.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011134 ID=SMED30011134|Name=SMED30011134|organism=Schmidtea mediterranea sexual|type=transcript|length=2107bp
AACCTTAGTTGAGGCAAATCTAGTTGCTTGACATAGTTAGTTGGTGTAAA
AACTTATTATATCACTTATTACAAAGACTGGGGTCCATTTTAGCCCAATG
CACAGACTGAAGGTATAAAGTATTAGTCAAAATATTGTTTAAAAATCCCA
AAATAATAATTTAAGAAAACATTTTTTGATGTTTAGTTAAAAATCTTTTT
GCATGTCAGCGTTTACCGACTCCTGCTTTAATAGTTTTATTTTATTGGAA
GTATAAGTTCGTGCTGATGTTGTCTGAATAAAATTGAATACATGACAGAG
CTTGACACTGTTTAAACCATATAAGAATATTTTGAAAAATATGAATTATG
AATGGTTTCTGTTTCAAACAAATTATATCAAAGATTTCCGCTTCCTGGCC
AGGTATATTAATGCAAGGATCCGATTGGCTGACCAGTGACACAAATTTTA
TGAATAAAAACATTCAACTTATTGACGGGCATAATCTTTTGCGATTTTAT
ACGAAAAGTTTTTTTAGAAATAATACAACGACCATTCCAAAACTAATCTC
AGTGAACCGGATCATACCGGGTTTTGACTAATTTTTTGATATTCTCCCTT
ATTTGTATCTAATTTTAGGTTAAGGAGGTCCGGATAAGACAAAATGTAAT
GATTTGATTGGTTGAATTTCGACCAGCCGGATTTATTATCTTATAAGCAG
TAACTCAAATATTGCATTATTTATTTGTGAAATGCCACAATGGATGAGTT
TTGCCAATGATTCCTGCAAATATTAAAATTGTTTAACCAAAAAAGGCAAT
GATAAGTCTCTTATTTTGAAAGCTTTTAATTTTTATCGCTTTCAGACAAT
ACCAGACCAAGTCGTCATTACTGAGTGTTCATATGAAAATATCTTTGACA
ACCTTTTATGATACAAAATCTTATTATCTTTTATCAGTGACTTGACAATT
AATTGGGAAGGAGTTATTTACGATACTTAAATATAATGTTTTCCCTATTT
AAGCCTGGCGCCATTTCTTAGCTATCAGATTACTCCACTTTTCTGTTGTT
TTACTGTAATAAATTTCGAATTTCCCAATCTTTAATTACTGATATCCATT
TATTTGTTTCAGATAAGAGCCAACAAGAGCTCCAGACTTTTCAGGCTTCT
TTCGACTCTTACAGACAAGCGCTGATAAAGGAGTCAGAGAACAAAATTGA
AATGGAAAAAGAACGCCTATTAGATTGTTGTAATCGTGATTTATCGGATA
AGCTGGACAAACAAAAAAAAGAATTGAAATGTAATTTTGAAGTTGATATT
GAAAGCATCAAGCAAGAGCACTCTTGTGAAATTGAGAAACTCAAATCGAA
CCACAAAACTGAAATCGATATACTGAATCGACGCTTTGCTGATGCCGTTG
CCGTCGAGGATAAGCTCAGCAAAACTCTACAAGAGGTTGAAAAAAGGAAA
CGAGATTATCAGAAATTGCAAGGAGAATATGAAAATTTGCACCAAGCATA
CTCCAAGATCCGAAGAGAGTTGACCATGTCCTTCAACGAAAATGAAAATT
TGAAATCTGAGATCCAAGAATTTTCAGAAAGATTTGATGAGCGTGTGAAA
AGAGTTGATGATGATTATAAACAGAGAATTGATTCTCTACAGAACCAAAT
AGTAGAAATGAGACGGGCTTACATGGCGAAATGCGAAGAGCTCGTCAATG
AGCGGAAAGAAGCCGATAAACAGTTGCACGGAAAAATTGAGTCTGCCAAA
GATGTCATGAAAGAAATTATCCACACTCGACAGCGTGCAAGAATCAGTCT
CGTGAGTTTGGAAAGCGATTCTGAAACTGAGGAATTTTGGAAAACTCGAA
ATAAAACAGAAGAAGCGAAATCCTTTCTGTCTTCTTTTGGCAGATTGCAA
AATGACTCACATAAAGTTCCAGCTGATACAAATAACTCCGACGTTTTCAT
AAGGCCAATTAGCGCACCGACAACCGAGATGGATGAGAAAAATGTCAAGA
TGCAGAGTTTTTTGCTCAATAAAGATATTTTATAATTTTCCTTGAAGGTT
TTAGAAAAATTAATTTTATTTGTTGAAATTTATTTATTGAATTTTATTGA
CCTTTCA
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protein sequence of SMED30011134-orf-1

>SMED30011134-orf-1 ID=SMED30011134-orf-1|Name=SMED30011134-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=278bp
MEKERLLDCCNRDLSDKLDKQKKELKCNFEVDIESIKQEHSCEIEKLKSN
HKTEIDILNRRFADAVAVEDKLSKTLQEVEKRKRDYQKLQGEYENLHQAY
SKIRRELTMSFNENENLKSEIQEFSERFDERVKRVDDDYKQRIDSLQNQI
VEMRRAYMAKCEELVNERKEADKQLHGKIESAKDVMKEIIHTRQRARISL
VSLESDSETEEFWKTRNKTEEAKSFLSSFGRLQNDSHKVPADTNNSDVFI
RPISAPTTEMDEKNVKMQSFLLNKDIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000418head
Vocabulary: INTERPRO
TermDefinition
IPR026674FLACC1
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 69..127
NoneNo IPR availableCOILSCoilCoilcoord: 136..174
NoneNo IPR availableGENE3DG3DSA:1.20.120.20coord: 12..157
e-value: 7.3E-7
score: 31.0
IPR026674Flagellum-associated coiled-coil domain-containing protein 1PANTHERPTHR21707UNCHARACTERIZEDcoord: 16..191