SMED30011131

Overview
NameSMED30011131
Smed IDSMED30011131
Length (bp)1213
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011131 (SMED30011131) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011131 (SMED30011131) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011131 (SMED30011131) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011131 (SMED30011131) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30011131

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011131 SmedASXL_016602SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
protonephridiaSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011131h1SMcG0004831 dd_Smed_v4_11192_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30011131h1SMcG0004831 dd_Smed_v4_11192_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30011131h1SMcG0004831 dd_Smed_v4_11192_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
epidermisSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ventral epidermisSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
dorsal region of the epidermisSMED30011131h1SMcG0018385 dd_Smed_v4_5794_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011131 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.013292:11133..15693 -5819
v31.030597:427..1610 -4734
v31.003151:49478..50616 +4332
v31.014296:9955..11122 +4303
Homology
[Close]
BLAST of SMED30011131 vs. Planmine SMEST
Match: SMESG000050000.1 (SMESG000050000.1)

HSP 1 Score: 509.22 bits (1310), Expect = 1.951e-178
Identity = 268/272 (98.53%), Postives = 268/272 (98.53%), Query Frame = 1 
Query:  181 MKLKXXXXXXXXXXXXXXXQYSFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            MKLKINILFICLIFVIILIQYSFG I NFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLE NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAY VTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM
Sbjct:    1 MKLKINILFICLIFVIILIQYSFGLIANFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLESNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYLVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 272

HSP 2 Score: 491.5 bits (1264), Expect = 1.590e-171
Identity = 241/250 (96.40%), Postives = 241/250 (96.40%), Query Frame = 1 
Query:  244 SFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF 993
            SFG I NFSQRNTNLSVIINVTGIEPQ AFYV VSESFC TSNLISFNSHNLSDTGQRLE NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPS IGGTILAY VTIGGQLNDSVCIKRWLLSC DCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF
Sbjct:  311 SFGLIANFSQRNTNLSVIINVTGIEPQTAFYVNVSESFCLTSNLISFNSHNLSDTGQRLESNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSGIGGTILAYLVTIGGQLNDSVCIKRWLLSCGDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF 560
[Close]
BLAST of SMED30011131 vs. Planmine SMEST
Match: SMESG000005243.1 (SMESG000005243.1)

HSP 1 Score: 492.656 bits (1267), Expect = 2.150e-176
Identity = 241/251 (96.02%), Postives = 244/251 (97.21%), Query Frame = 1 
Query:  241 YSFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF 993
            +SFG + NFSQRNTNLSVIINVTGIEPQ AFYVKVSESFCFTSNLISFNSHNLSDTGQ LE NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYAN TWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAY VT+GGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF
Sbjct:   13 HSFGLVANFSQRNTNLSVIINVTGIEPQTAFYVKVSESFCFTSNLISFNSHNLSDTGQLLESNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANLTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYLVTMGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF 263
[Close]
BLAST of SMED30011131 vs. Planmine SMEST
Match: SMESG000005237.1 (SMESG000005237.1)

HSP 1 Score: 498.434 bits (1282), Expect = 2.084e-173
Identity = 263/272 (96.69%), Postives = 265/272 (97.43%), Query Frame = 1 
Query:  181 MKLKXXXXXXXXXXXXXXXQYSFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            MKLKINILFICLIFVIILIQYSFG I NFSQRN NLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQ LEKNCTLVNYLNGNIVNG VYATFNICCIVSG+MYEISINY NSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAY VTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF+
Sbjct:    1 MKLKINILFICLIFVIILIQYSFGLIANFSQRNANLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQLLEKNCTLVNYLNGNIVNGHVYATFNICCIVSGKMYEISINYVNSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYLVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFI 272

HSP 2 Score: 66.2402 bits (160), Expect = 1.802e-11
Identity = 67/247 (27.13%), Postives = 113/247 (45.75%), Query Frame = 1 
Query:  430 CTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM---------YSNGGSFNPGVDYRRPVLTIFPDCEIHQTFRKLFYNGQSIENQCI 1131
            C  +N LN N  +     T N   I+S   Y I+I   N   W   ++ T  +    S PV N+   S +    L I W  P    G I+ YF+ +    N+  C+    L+C+DC+ +       + + S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ TDQ++          S+  SF   + +  PV++   + EI Q F  +    Q+  N+C+
Sbjct:  343 CYTINGLNFNSNDKSFSMTINDGKILSYTNYTINIRSLNGYGWGNLSSKTIKTNQYISGPVTNVTVNSSFPSFPLSITWAPPGVTNGEIVQYFIWVQNTNNE--CVLTMSLNCTDCKNNLSV--PRMPENSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYISGPVTNLSVTSSFPSFPLSIIWSPPVVS---NGEIVQYFIWV----QNTNNECV 576
[Close]
BLAST of SMED30011131 vs. Planmine SMEST
Match: SMESG000005237.1 (SMESG000005237.1)

HSP 1 Score: 496.893 bits (1278), Expect = 2.620e-164
Identity = 263/272 (96.69%), Postives = 265/272 (97.43%), Query Frame = 1 
Query:  181 MKLKXXXXXXXXXXXXXXXQYSFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            MKLKINILFICLIFVIILIQYSFG I NFSQRN NLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQ LEKNCTLVNYLNGNIVNG VYATFNICCIVSG+MYEISINY NSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAY VTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF+
Sbjct:    1 MKLKINILFICLIFVIILIQYSFGLIANFSQRNANLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQLLEKNCTLVNYLNGNIVNGHVYATFNICCIVSGKMYEISINYVNSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYLVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFI 272

HSP 2 Score: 71.633 bits (174), Expect = 4.140e-13
Identity = 64/210 (30.48%), Postives = 99/210 (47.14%), Query Frame = 1 
Query:  427 NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFMYSNGGSFNPGVDYRRP 1044
            NC  +N LN N  N     T N   I+    Y I+I   N   W  S++ T  +    S PV NL+ TS +    L I W  P    G I+ YF+ +    N+  C+    L+C+DC+ +       + + S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ TDQ  Y++G   N  V    P
Sbjct:  472 NCYTINGLNFNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYISGPVTNLSVTSSFPSFPLSIIWSPPVVSNGEIVQYFIWVQNTNNE--CVLTMSLNCTDCKNNLSV--PRIPENSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQ--YTSGSIMNLSVASSFP 673

HSP 3 Score: 67.0106 bits (162), Expect = 1.312e-11
Identity = 56/202 (27.72%), Postives = 95/202 (47.03%), Query Frame = 1 
Query:  427 NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFMYSNGGSFN 1020
            NC  +N LN N  N     T N   I+    Y I+I   N   W  S++ T  +    S  + NL+  S +    L I W+ P    G I+ Y + +    N+  C+     +C+DC+ +      ++ D S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ T+Q++     +FN
Sbjct:  602 NCYTINGLNFNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYTSGSIMNLSVASSFPSFPLSITWEPPGVANGEIVQYLIWVQNTNNE--CVLTVSFNCTDCKNNLSV--PAMPDNSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINVRSLNGYGWGNSSSKTIQTNQYISGPVSNFN 797

HSP 4 Score: 66.6254 bits (161), Expect = 1.725e-11
Identity = 55/193 (28.50%), Postives = 91/193 (47.15%), Query Frame = 1 
Query:  430 CTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            C  +N LN N  +     T N   I+S   Y I+I   N   W   ++ T  +    S PV N+   S +    L I W  P    G I+ YF+ +    N+  C+    L+C+DC+ +       + + S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ TDQ++
Sbjct:  343 CYTINGLNFNSNDKSFSMTINDGKILSYTNYTINIRSLNGYGWGNLSSKTIKTNQYISGPVTNVTVNSSFPSFPLSITWAPPGVTNGEIVQYFIWVQNTNNE--CVLTMSLNCTDCKNNLSV--PRMPENSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYI 529
[Close]
BLAST of SMED30011131 vs. Planmine SMEST
Match: SMESG000005237.1 (SMESG000005237.1)

HSP 1 Score: 497.278 bits (1279), Expect = 3.324e-164
Identity = 263/272 (96.69%), Postives = 265/272 (97.43%), Query Frame = 1 
Query:  181 MKLKXXXXXXXXXXXXXXXQYSFGFIVNFSQRNTNLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            MKLKINILFICLIFVIILIQYSFG I NFSQRN NLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQ LEKNCTLVNYLNGNIVNG VYATFNICCIVSG+MYEISINY NSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAY VTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQF+
Sbjct:    1 MKLKINILFICLIFVIILIQYSFGLIANFSQRNANLSVIINVTGIEPQNAFYVKVSESFCFTSNLISFNSHNLSDTGQLLEKNCTLVNYLNGNIVNGHVYATFNICCIVSGKMYEISINYVNSTWRESANITAFSKSPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYLVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFI 272

HSP 2 Score: 72.0182 bits (175), Expect = 3.454e-13
Identity = 64/210 (30.48%), Postives = 99/210 (47.14%), Query Frame = 1 
Query:  427 NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFMYSNGGSFNPGVDYRRP 1044
            NC  +N LN N  N     T N   I+    Y I+I   N   W  S++ T  +    S PV NL+ TS +    L I W  P    G I+ YF+ +    N+  C+    L+C+DC+ +       + + S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ TDQ  Y++G   N  V    P
Sbjct:  472 NCYTINGLNFNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYISGPVTNLSVTSSFPSFPLSIIWSPPVVSNGEIVQYFIWVQNTNNE--CVLTMSLNCTDCKNNLSV--PRIPENSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQ--YTSGSIMNLSVASSFP 673

HSP 3 Score: 67.0106 bits (162), Expect = 1.166e-11
Identity = 56/202 (27.72%), Postives = 95/202 (47.03%), Query Frame = 1 
Query:  427 NCTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFMYSNGGSFN 1020
            NC  +N LN N  N     T N   I+    Y I+I   N   W  S++ T  +    S  + NL+  S +    L I W+ P    G I+ Y + +    N+  C+     +C+DC+ +      ++ D S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ T+Q++     +FN
Sbjct:  602 NCYTINGLNFNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYTSGSIMNLSVASSFPSFPLSITWEPPGVANGEIVQYLIWVQNTNNE--CVLTVSFNCTDCKNNLSV--PAMPDNSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINVRSLNGYGWGNSSSKTIQTNQYISGPVSNFN 797

HSP 4 Score: 66.6254 bits (161), Expect = 1.505e-11
Identity = 55/193 (28.50%), Postives = 91/193 (47.15%), Query Frame = 1 
Query:  430 CTLVNYLNGNIVNGQVYATFNICCIVSGQMYEISINYANS-TWRESANITAFSK---SPPVQNLNGTSKWGVNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQAIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIATALNEFGWGDAQSISLLTDQFM 996
            C  +N LN N  +     T N   I+S   Y I+I   N   W   ++ T  +    S PV N+   S +    L I W  P    G I+ YF+ +    N+  C+    L+C+DC+ +       + + S  NC  IN  +   ++ +K  S+  ND  + P T+Y +   +LN +GWG++ S ++ TDQ++
Sbjct:  343 CYTINGLNFNSNDKSFSMTINDGKILSYTNYTINIRSLNGYGWGNLSSKTIKTNQYISGPVTNVTVNSSFPSFPLSITWAPPGVTNGEIVQYFIWVQNTNNE--CVLTMSLNCTDCKNNLSV--PRMPENSTDNCYTINGLN--FNSNNKSFSMTINDGKILPYTNYTINIRSLNGYGWGNSSSKTIQTDQYI 529
The following BLAST results are available for this feature:
BLAST of SMED30011131 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011131 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000050000.11.951e-17898.53SMESG000050000.1[more]
SMESG000005243.12.150e-17696.02SMESG000005243.1[more]
SMESG000005237.12.084e-17396.69SMESG000005237.1[more]
SMESG000005237.12.620e-16496.69SMESG000005237.1[more]
SMESG000005237.13.324e-16496.69SMESG000005237.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011131 ID=SMED30011131|Name=SMED30011131|organism=Schmidtea mediterranea sexual|type=transcript|length=1213bp
GATTCGTATTTCTTTGATGTAAAATTGATAAATTTGTTTGAATATGTCAA
ATAAAATAAAAGTTAGCGAGCAACTTATAAATTATAAATCTAATATCATT
TAATTATATTATAATATACTAAAGAAATCGAGATATTATTATTTGATACT
ATTATTAACCGGTACTTTATCAGTATTTAAATGAAATTGAAGATTAATAT
TTTGTTTATATGTCTTATTTTTGTTATTATTCTAATACAATATTCATTTG
GATTTATTGTAAATTTTTCTCAAAGAAATACGAATCTCTCAGTTATAATT
AATGTAACTGGCATTGAACCACAAAATGCGTTCTATGTCAAAGTTTCCGA
ATCATTTTGCTTTACCAGCAACCTAATATCTTTCAATAGTCATAATCTAT
CAGACACTGGCCAGCGCCTGGAGAAAAATTGCACTCTTGTAAATTATCTC
AACGGGAACATAGTCAATGGGCAAGTGTATGCAACATTCAACATATGCTG
CATTGTCAGTGGGCAAATGTATGAAATCTCAATAAATTATGCCAACTCAA
CATGGAGAGAATCTGCCAACATAACTGCCTTCAGTAAAAGTCCACCAGTG
CAAAACTTGAACGGAACTAGCAAATGGGGCGTGAATAATCTCCTGATCGA
ATGGGATCGTCCATCGGCGATTGGAGGGACAATTCTCGCGTATTTTGTAA
CGATCGGTGGGCAATTAAACGATTCAGTTTGCATCAAACGCTGGCTGCTC
AGTTGCAGTGATTGCCAGGAATCAGCGCAGGCGATCGGGCAGAGTTTGCA
GGATCCTTCAGTAAGGAATTGCAGCCAGATCAATAATGCCAGCTCATTCG
TGTCGACCGCTGATAAGCATTTATCACTGGCAACTAATGATTCGGATGTT
AAACCGGCCACGAGTTATCTCGTGATTGCGACTGCTCTCAATGAATTCGG
CTGGGGAGACGCGCAGTCAATTTCACTGCTGACGGACCAATTCATGTATT
CTAATGGCGGTTCATTTAACCCAGGGGTCGATTACCGACGGCCCGTCTTG
ACAATTTTTCCGGACTGCGAGATTCATCAGACTTTTAGAAAATTATTTTA
TAATGGCCAAAGTATTGAAAACCAATGTATTAAAAAAATACATTCATATA
TTCAAAAATTGATTGTTGAAATTATGAAAAAAAAATAACTTTGATTTAAA
AATAATATTCTTG
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protein sequence of SMED30011131-orf-1

>SMED30011131-orf-1 ID=SMED30011131-orf-1|Name=SMED30011131-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=336bp
MKLKINILFICLIFVIILIQYSFGFIVNFSQRNTNLSVIINVTGIEPQNA
FYVKVSESFCFTSNLISFNSHNLSDTGQRLEKNCTLVNYLNGNIVNGQVY
ATFNICCIVSGQMYEISINYANSTWRESANITAFSKSPPVQNLNGTSKWG
VNNLLIEWDRPSAIGGTILAYFVTIGGQLNDSVCIKRWLLSCSDCQESAQ
AIGQSLQDPSVRNCSQINNASSFVSTADKHLSLATNDSDVKPATSYLVIA
TALNEFGWGDAQSISLLTDQFMYSNGGSFNPGVDYRRPVLTIFPDCEIHQ
TFRKLFYNGQSIENQCIKKIHSYIQKLIVEIMKKK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000054dorsal region of the epidermis
PLANA:0000106ciliated epithelial cell
Vocabulary: INTERPRO
TermDefinition
IPR036116FN3_sf
IPR013783Ig-like_fold
IPR003961FN3_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 121..270
e-value: 6.8E-6
score: 28.1
IPR036116Fibronectin type III superfamilySUPERFAMILYSSF49265Fibronectin type IIIcoord: 102..265
NoneNo IPR availableTMHMMTMhelixcoord: 5..27