ZP domain-containing protein

Overview
NameZP domain-containing protein
Smed IDSMED30011122
Length (bp)241
Neoblast Clusters

Zeng et. al., 2018




 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011122

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30011122h1SMcG0004667 SmedASXL_012021SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
head regionSMED30011122h1SMcG0004667 SmedASXL_005104SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016
whole organism asexual adult RNA-sequencing evidence
Smed sexual biotypeSMED30011122h1SMcG0004667 Contig24987newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
nervous systemSMED30011122h1SMcG0004667 dd_Smed_v4_13730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30011122h1SMcG0004667 dd_Smed_v4_13730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30011122h1SMcG0004667 dd_Smed_v4_13730_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30011122h1SMcG0004667 dd_Smed_v6_13730_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A183RRE8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 6.300e-9
Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3
Query:  105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
            CGIR YK++H  +QYE ILEVL N S +TPS+D   PL CI++ P
Sbjct:   91 CGIRLYKLSHDTIQYEGILEVLVNTSIRTPSRDKIIPLKCISRIP 135          
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A5K4F052 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 7.445e-9
Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3
Query:  105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
            CGIR YK++H  +QYE ILEVL N S +TPS+D   PL CI++ P
Sbjct:  452 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 496          
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A5K4F1C8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 7.819e-9
Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3
Query:  105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
            CGIR YK++H  +QYE ILEVL N S +TPS+D   PL CI++ P
Sbjct:  464 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 508          
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A074Z660 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_10545 PE=4 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.264e-8
Identity = 22/46 (47.83%), Postives = 34/46 (73.91%), Query Frame = 3
Query:  102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
            +CG+R Y++    +QYE +LE+L N+S +TPS+D+Q P+ CI  EP
Sbjct:  537 QCGVRLYRLGRNVIQYEALLEILVNRSVRTPSQDLQIPIKCIAPEP 582          
BLAST of ZP domain-containing protein vs. TrEMBL
Match: G4VRN9 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_167790 PE=4 SV=1)

HSP 1 Score: 59.6918 bits (143), Expect = 1.929e-8
Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3
Query:  105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
            CGIR YK++H  +QYE ILEVL N S +TPS+D   PL CI++ P
Sbjct:  395 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 439          
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000039228.1 (SMESG000039228.1)

HSP 1 Score: 105.531 bits (262), Expect = 2.654e-28
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 3
Query:   84 FIRQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
             + QIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP
Sbjct:  379 LLEQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 430          

HSP 2 Score: 68.5514 bits (166), Expect = 3.593e-15
Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 1
Query:    1 IGGSDFKANSECFLNFVEDENQFCINIDLLDKLENAAFGRIKL 129
            IGGSDFKANSECFLNFVEDENQFCINIDLL+++        K+
Sbjct:  351 IGGSDFKANSECFLNFVEDENQFCINIDLLEQIRECGIRSYKI 393          
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000039228.1 (SMESG000039228.1)

HSP 1 Score: 105.916 bits (263), Expect = 3.134e-28
Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 3
Query:   84 FIRQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239
             + QIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP
Sbjct:  379 LLEQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 430          

HSP 2 Score: 69.3218 bits (168), Expect = 1.989e-15
Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 1
Query:    1 IGGSDFKANSECFLNFVEDENQFCINIDLLDKLENAAFGRIKL 129
            IGGSDFKANSECFLNFVEDENQFCINIDLL+++        K+
Sbjct:  351 IGGSDFKANSECFLNFVEDENQFCINIDLLEQIRECGIRSYKI 393          
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000020512.1 (SMESG000020512.1)

HSP 1 Score: 41.5874 bits (96), Expect = 3.668e-6
Identity = 18/45 (40.00%), Postives = 29/45 (64.44%), Query Frame = 3
Query:  102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKE 236
             CG++ +K+   KL Y+  LEV  +++ K PSKD+  P+SCI+  
Sbjct:  363 NCGVQMHKLKFGKLLYQATLEVRLDRNIKQPSKDLVIPVSCISDH 407          

HSP 2 Score: 25.0238 bits (53), Expect = 3.668e-6
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1
Query:   31 ECFLNFVEDENQFCINIDLLD 93
            +C L  +   N++CI IDLLD
Sbjct:  339 KCNLQKIASYNKYCIRIDLLD 359          
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000020512.1 (SMESG000020512.1)

HSP 1 Score: 41.5874 bits (96), Expect = 3.940e-6
Identity = 18/45 (40.00%), Postives = 29/45 (64.44%), Query Frame = 3
Query:  102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKE 236
             CG++ +K+   KL Y+  LEV  +++ K PSKD+  P+SCI+  
Sbjct:  383 NCGVQMHKLKFGKLLYQATLEVRLDRNIKQPSKDLVIPVSCISDH 427          

HSP 2 Score: 24.6386 bits (52), Expect = 3.940e-6
Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1
Query:   31 ECFLNFVEDENQFCINIDLLD 93
            +C L  +   N++CI IDLLD
Sbjct:  359 KCNLQKIASYNKYCIRIDLLD 379          
The following BLAST results are available for this feature:
BLAST of ZP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183RRE86.300e-957.78Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
A0A5K4F0527.445e-957.78Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A5K4F1C87.819e-957.78Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A074Z6601.264e-847.83Uncharacterized protein OS=Opisthorchis viverrini ... [more]
G4VRN91.929e-857.78Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
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BLAST of ZP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of ZP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000039228.12.654e-2894.23SMESG000039228.1[more]
SMESG000039228.13.134e-2894.23SMESG000039228.1[more]
SMESG000020512.13.668e-640.00SMESG000020512.1[more]
SMESG000020512.13.940e-640.00SMESG000020512.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011122 ID=SMED30011122|Name=ZP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=241bp
ATCGGTGGCAGCGATTTCAAAGCCAATTCCGAATGTTTTCTCAATTTTGT
AGAAGATGAAAATCAGTTCTGTATAAATATAGATTTATTAGACAAATTAG
AGAATGCGGCATTCGGTCGTATAAAATTGCACATAGAAAACTCCAATATG
AATTTATTCTGGAAGTCCTCAGTAATAAATCATTCAAAACTCCATCAAAG
GACATGCAGTTCCCATTATCGTGCATTACAAAAGAACCAGA
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000418head
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001507ZP_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001507Zona pellucida domainPROSITEPS51034ZP_2coord: 1..80
score: 8.731