ZP domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011122 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A183RRE8 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 6.300e-9 Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3 Query: 105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 CGIR YK++H +QYE ILEVL N S +TPS+D PL CI++ P Sbjct: 91 CGIRLYKLSHDTIQYEGILEVLVNTSIRTPSRDKIIPLKCISRIP 135
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A5K4F052 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 7.445e-9 Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3 Query: 105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 CGIR YK++H +QYE ILEVL N S +TPS+D PL CI++ P Sbjct: 452 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 496
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A5K4F1C8 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 60.8474 bits (146), Expect = 7.819e-9 Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3 Query: 105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 CGIR YK++H +QYE ILEVL N S +TPS+D PL CI++ P Sbjct: 464 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 508
BLAST of ZP domain-containing protein vs. TrEMBL
Match: A0A074Z660 (Uncharacterized protein OS=Opisthorchis viverrini OX=6198 GN=T265_10545 PE=4 SV=1) HSP 1 Score: 60.077 bits (144), Expect = 1.264e-8 Identity = 22/46 (47.83%), Postives = 34/46 (73.91%), Query Frame = 3 Query: 102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 +CG+R Y++ +QYE +LE+L N+S +TPS+D+Q P+ CI EP Sbjct: 537 QCGVRLYRLGRNVIQYEALLEILVNRSVRTPSQDLQIPIKCIAPEP 582
BLAST of ZP domain-containing protein vs. TrEMBL
Match: G4VRN9 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_167790 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 1.929e-8 Identity = 26/45 (57.78%), Postives = 33/45 (73.33%), Query Frame = 3 Query: 105 CGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 CGIR YK++H +QYE ILEVL N S +TPS+D PL CI++ P Sbjct: 395 CGIRLYKLSHDTVQYEGILEVLVNTSIRTPSRDKIIPLKCISRTP 439
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000039228.1 (SMESG000039228.1) HSP 1 Score: 105.531 bits (262), Expect = 2.654e-28 Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 3 Query: 84 FIRQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 + QIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP Sbjct: 379 LLEQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 430 HSP 2 Score: 68.5514 bits (166), Expect = 3.593e-15 Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 1 Query: 1 IGGSDFKANSECFLNFVEDENQFCINIDLLDKLENAAFGRIKL 129 IGGSDFKANSECFLNFVEDENQFCINIDLL+++ K+ Sbjct: 351 IGGSDFKANSECFLNFVEDENQFCINIDLLEQIRECGIRSYKI 393
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000039228.1 (SMESG000039228.1) HSP 1 Score: 105.916 bits (263), Expect = 3.134e-28 Identity = 49/52 (94.23%), Postives = 50/52 (96.15%), Query Frame = 3 Query: 84 FIRQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 239 + QIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP Sbjct: 379 LLEQIRECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKEP 430 HSP 2 Score: 69.3218 bits (168), Expect = 1.989e-15 Identity = 31/43 (72.09%), Postives = 35/43 (81.40%), Query Frame = 1 Query: 1 IGGSDFKANSECFLNFVEDENQFCINIDLLDKLENAAFGRIKL 129 IGGSDFKANSECFLNFVEDENQFCINIDLL+++ K+ Sbjct: 351 IGGSDFKANSECFLNFVEDENQFCINIDLLEQIRECGIRSYKI 393
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000020512.1 (SMESG000020512.1) HSP 1 Score: 41.5874 bits (96), Expect = 3.668e-6 Identity = 18/45 (40.00%), Postives = 29/45 (64.44%), Query Frame = 3 Query: 102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKE 236 CG++ +K+ KL Y+ LEV +++ K PSKD+ P+SCI+ Sbjct: 363 NCGVQMHKLKFGKLLYQATLEVRLDRNIKQPSKDLVIPVSCISDH 407 HSP 2 Score: 25.0238 bits (53), Expect = 3.668e-6 Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1 Query: 31 ECFLNFVEDENQFCINIDLLD 93 +C L + N++CI IDLLD Sbjct: 339 KCNLQKIASYNKYCIRIDLLD 359
BLAST of ZP domain-containing protein vs. Planmine SMEST
Match: SMESG000020512.1 (SMESG000020512.1) HSP 1 Score: 41.5874 bits (96), Expect = 3.940e-6 Identity = 18/45 (40.00%), Postives = 29/45 (64.44%), Query Frame = 3 Query: 102 ECGIRSYKIAHRKLQYEFILEVLSNKSFKTPSKDMQFPLSCITKE 236 CG++ +K+ KL Y+ LEV +++ K PSKD+ P+SCI+ Sbjct: 383 NCGVQMHKLKFGKLLYQATLEVRLDRNIKQPSKDLVIPVSCISDH 427 HSP 2 Score: 24.6386 bits (52), Expect = 3.940e-6 Identity = 10/21 (47.62%), Postives = 14/21 (66.67%), Query Frame = 1 Query: 31 ECFLNFVEDENQFCINIDLLD 93 +C L + N++CI IDLLD Sbjct: 359 KCNLQKIASYNKYCIRIDLLD 379 The following BLAST results are available for this feature:
BLAST of ZP domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of ZP domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of ZP domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of ZP domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of ZP domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 4
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011122 ID=SMED30011122|Name=ZP domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=241bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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