SMED30011058

Overview
NameSMED30011058
Smed IDSMED30011058
Length (bp)1343
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30011058 (SMED30011058) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30011058 (SMED30011058) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30011058 (SMED30011058) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30011058 (SMED30011058) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30011058

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 5

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30011058SMESG000042000.1 dd_Smed_v4_3000_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
nervous systemSMED30011058SMESG000042000.1 dd_Smed_v4_3000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011058SMESG000042000.1 Contig55658newmark_estsPMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30011058SMESG000042000.1 Contig55658uc_Smed_v2PMID:29674431
Fincher et al., 2018
FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30011058SMESG000042000.1 OX_Smed_1.0.15795ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30011058 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000608:36533..48843 +6231
Homology
BLAST of SMED30011058 vs. TrEMBL
Match: K1PDZ5 (Uncharacterized protein OS=Crassostrea gigas OX=29159 GN=CGI_10007565 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.035e-7
Identity = 40/127 (31.50%), Postives = 61/127 (48.03%), Query Frame = 2
Query:  617 FVCPWDD---PSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLV-FVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPNA----VENKPVQSVLI-TPGQDQHDPLIQSDNC-PPYPQINQPYPM 967
            F CP D+     YRYCCG+E Q+ CC+ +++PGH+ G++ G++   VA  I+      C   +  R+  + + V  A         V   P   +    P +      + + NC PPYP  N PYPM
Sbjct:   13 FFCPIDEYMSEDYRYCCGEEGQQNCCTFWDKPGHIVGVVFGIIAGMVALFIAVFCVVKCIRTKKSRNCDYASTVSNASKEQVIYPPVYAPPTYEIACGIPNEKTPFAKMDAGNCNPPYPPSNVPYPM 139          
BLAST of SMED30011058 vs. TrEMBL
Match: A0A1S3HKT4 (uncharacterized protein LOC106155682 OS=Lingula unguis OX=7574 GN=LOC106155682 PE=4 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 8.095e-6
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 2
Query:  566 CYNEFPLYDRNISATPWFVCP-WDDP-SYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAF 751
            CYN    +  N S    F+CP WD P    YCCG E+Q+ CC  ++  G +AG+++G+L+ V  
Sbjct:   10 CYNSARRFQENGSLFNEFICPEWDQPDELEYCCGPELQQYCCGFWDDGGRVAGVVIGILIAVTL 73          
BLAST of SMED30011058 vs. TrEMBL
Match: A0A1S3HM18 (uncharacterized protein LOC106155680 OS=Lingula unguis OX=7574 GN=LOC106155680 PE=4 SV=1)

HSP 1 Score: 58.151 bits (139), Expect = 9.442e-6
Identity = 33/94 (35.11%), Postives = 46/94 (48.94%), Query Frame = 2
Query:  476 GISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCP-WDDP-SYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAF 751
            G   GL     R R     DRE +      CYN    +  N S    F+CP WD P    YCCG E+Q+ CC  ++  G +AG+++G+L+ V  
Sbjct:   55 GTVYGLSRYQRRRRYKMDPDREPTR-----CYNSARRFQENGSLFNEFICPEWDQPDELEYCCGPELQQYCCGFWDDGGRVAGVVIGILIAVTL 143          
BLAST of SMED30011058 vs. Planmine SMEST
Match: SMESG000042000.1 (SMESG000042000.1)

HSP 1 Score: 402.905 bits (1034), Expect = 5.895e-139
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 2
Query:  299 KTQVKVKVKIQSKNKXXXXXXXXXXXXXXXXXXXXXWSRYHGFQPNFXXXXXXXXXXXXGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSXXXXXXXXXXXXXXXXXXXXXXXXXXXMGYEQPNQFNQXXXXXXXXXXXXXXXXXXEAAKSHY 1192
            KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY
Sbjct:   57 KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY 354          
BLAST of SMED30011058 vs. Planmine SMEST
Match: SMESG000042000.1 (SMESG000042000.1)

HSP 1 Score: 402.134 bits (1032), Expect = 1.208e-138
Identity = 298/298 (100.00%), Postives = 298/298 (100.00%), Query Frame = 2
Query:  299 KTQVKVKVKIQSKNKXXXXXXXXXXXXXXXXXXXXXWSRYHGFQPNFXXXXXXXXXXXXGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSXXXXXXXXXXXXXXXXXXXXXXXXXXXMGYEQPNQFNQXXXXXXXXXXXXXXXXXXEAAKSHY 1192
            KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY
Sbjct:   62 KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY 359          
BLAST of SMED30011058 vs. Planmine SMEST
Match: SMESG000042000.1 (SMESG000042000.1)

HSP 1 Score: 398.282 bits (1022), Expect = 3.592e-137
Identity = 298/299 (99.67%), Postives = 298/299 (99.67%), Query Frame = 2
Query:  299 KTQVKVKVKIQSKNKXXXXXXXXXXXXXXXXXXXXXWSRYHGFQPNFXXXXXXXXXXXXGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPN-AVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSXXXXXXXXXXXXXXXXXXXXXXXXXXXMGYEQPNQFNQXXXXXXXXXXXXXXXXXXEAAKSHY 1192
            KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPN AVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY
Sbjct:   57 KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNEAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY 355          
BLAST of SMED30011058 vs. Planmine SMEST
Match: SMESG000042000.1 (SMESG000042000.1)

HSP 1 Score: 397.512 bits (1020), Expect = 8.850e-137
Identity = 298/299 (99.67%), Postives = 298/299 (99.67%), Query Frame = 2
Query:  299 KTQVKVKVKIQSKNKXXXXXXXXXXXXXXXXXXXXXWSRYHGFQPNFXXXXXXXXXXXXGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPN-AVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSXXXXXXXXXXXXXXXXXXXXXXXXXXXMGYEQPNQFNQXXXXXXXXXXXXXXXXXXEAAKSHY 1192
            KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPN AVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY
Sbjct:   62 KTQVKVKVKIQSKNKYGPSYRSGSYSRIGYSSRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPNPNEAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGDNYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSSYSDNSLPSYSEAAKSHY 360          
BLAST of SMED30011058 vs. Planmine SMEST
Match: SMESG000042003.1 (SMESG000042003.1)

HSP 1 Score: 156.762 bits (395), Expect = 4.564e-44
Identity = 87/154 (56.49%), Postives = 105/154 (68.18%), Query Frame = 2
Query:  407 WSRYHGFQPNFXXXXXXXXXXXXGISMGLIIMSHRHRNTFIIDRESSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSNYERPGHLAGLIVGLLVFVAFXXXXXXXXXXXXXRNKRDKSHVTLVYTAPNPNAVENKPV 868
            WSR HG   +FRYRSSSY YK  GI  GLIIMS   R TF      SYNDISICYNEFPLYDRN+S +PWFVCPW DP YRYCCGDE++ERCC  ++RPGHLAGLI+G+++  A I  AI  C+      +R  S V   + +P    ++N P+
Sbjct:   73 WSRSHGLSSSFRYRSSSYSYKRTGIITGLIIMSDYQRRTFADQYNKSYNDISICYNEFPLYDRNVSISPWFVCPWKDPRYRYCCGDEIEERCCRYFDRPGHLAGLIIGVIILCALIYIAIKYCHVMIKHCRRSCSKVVYKHDSPKNAKLDNNPI 226          
The following BLAST results are available for this feature:
BLAST of SMED30011058 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 3
Match NameE-valueIdentityDescription
K1PDZ53.035e-731.50Uncharacterized protein OS=Crassostrea gigas OX=29... [more]
A0A1S3HKT48.095e-639.06uncharacterized protein LOC106155682 OS=Lingula un... [more]
A0A1S3HM189.442e-635.11uncharacterized protein LOC106155680 OS=Lingula un... [more]
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BLAST of SMED30011058 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30011058 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042000.15.895e-139100.00SMESG000042000.1[more]
SMESG000042000.11.208e-138100.00SMESG000042000.1[more]
SMESG000042000.13.592e-13799.67SMESG000042000.1[more]
SMESG000042000.18.850e-13799.67SMESG000042000.1[more]
SMESG000042003.14.564e-4456.49SMESG000042003.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30011058 ID=SMED30011058|Name=SMED30011058|organism=Schmidtea mediterranea sexual|type=transcript|length=1343bp
CTAAAACCTAAATATAGATGGAATTTAAGTAATAAATTTAAATTGAATTA
TTCAATAGACATTTTAGTTTCTGAGTTATCTGTTTTAAGAAATGCTACTT
AATTGGATGATCCGCACTTTTTATATTACATTTTTTATATTTTTTATTAT
ATTTGAATTTTATATTACTTCTGGAATATCTCGTGGAGGAGGATCTCGTG
GTGGTAGTGGTGGATATCGAAGTGGTAGCAGTGGAGGGTATCGAAGTGGG
AGCAGTGGAGGGTATCGAAGTGGGAGCAGTGGGTATAGAAGTGGTAGCAA
AACTCAAGTTAAGGTTAAGGTTAAGATTCAATCAAAAAATAAATATGGCC
CTAGTTATCGAAGTGGGTCATATAGCAGAATAGGATATTCTTCCAGATAT
AATTCTTGGTCTCGCTATCATGGATTTCAGCCAAATTTTCGTTATCGGTC
CAGTTCTTATTATTATAAAAGTAGAGGGATTTCAATGGGTCTTATTATTA
TGTCTCACCGTCACAGAAATACATTTATCATTGATCGCGAATCATCTTAT
AATGATATTTCAATATGTTATAATGAGTTCCCACTTTATGATCGCAACAT
TTCTGCAACACCTTGGTTTGTATGTCCATGGGATGATCCAAGCTATAGAT
ACTGTTGTGGTGACGAAATGCAAGAGCGGTGCTGTTCAAACTATGAAAGG
CCGGGGCATCTAGCAGGATTGATAGTTGGATTATTAGTTTTTGTGGCTTT
TATTATTTCAGCAATAATATGTTGTTATTGCTGCTGTTGTCGCAATAAAA
GAGATAAATCTCACGTGACATTGGTATACACTGCACCTAACCCGAATGCT
GTAGAAAATAAACCAGTGCAATCTGTTTTAATAACTCCTGGCCAAGATCA
GCATGATCCTCTAATACAGTCAGATAATTGCCCTCCTTATCCCCAGATCA
ATCAACCATATCCGATGTATCCTCCTTGCCCTACAGGTGATAACTATAAT
TCGCCTCCACAGCCTCAACCAAATATTCCATATCCACCACCCAATCCATA
TGGCCAGGGAGCTCCGCCGTATCCCCCGAATGCAATGGGATACGAGCAGC
CTAATCAATTTAATCAATCTGCACCGCCTGGCAGCAGTAGTTATTCAGAC
AATTCATTACCATCTTACTCAGAGGCTGCAAAAAGTCACTACTGATGTTA
ACGTTCAATATTTAAATTTATTTATTTTATTTATAATCCCACATGTTTCT
CGTCTAGTCAATTTATTGTATGTCATTGTGATAGAATTTTATTAGTAAAT
TTTTGTCAATGGTAAATATATATATATATGCACTTTAATAACC
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protein sequence of SMED30011058-orf-1

>SMED30011058-orf-1 ID=SMED30011058-orf-1|Name=SMED30011058-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=368bp
MLLNWMIRTFYITFFIFFIIFEFYITSGISRGGGSRGGSGGYRSGSSGGY
RSGSSGGYRSGSSGYRSGSKTQVKVKVKIQSKNKYGPSYRSGSYSRIGYS
SRYNSWSRYHGFQPNFRYRSSSYYYKSRGISMGLIIMSHRHRNTFIIDRE
SSYNDISICYNEFPLYDRNISATPWFVCPWDDPSYRYCCGDEMQERCCSN
YERPGHLAGLIVGLLVFVAFIISAIICCYCCCCRNKRDKSHVTLVYTAPN
PNAVENKPVQSVLITPGQDQHDPLIQSDNCPPYPQINQPYPMYPPCPTGD
NYNSPPQPQPNIPYPPPNPYGQGAPPYPPNAMGYEQPNQFNQSAPPGSSS
YSDNSLPSYSEAAKSHY*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002056medial muscle cell
Vocabulary: INTERPRO
TermDefinition
IPR026910Shisa
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 36..64
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 299..324
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 299..359
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 40..64
NoneNo IPR availablePANTHERPTHR31395:SF14PROTEIN SHISA-5coord: 176..353
NoneNo IPR availableTMHMMTMhelixcoord: 7..29
NoneNo IPR availableTMHMMTMhelixcoord: 199..221
IPR026910Shisa familyPANTHERPTHR31395FAMILY NOT NAMEDcoord: 176..353