SMED30011056
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011056 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30011056 aligns in the following genomic locations:
Homology
BLAST of SMED30011056 vs. TrEMBL
Match: A0A368FHD9 (Uncharacterized protein (Fragment) OS=Ancylostoma caninum OX=29170 GN=ANCCAN_22634 PE=4 SV=1) HSP 1 Score: 77.0258 bits (188), Expect = 1.899e-12 Identity = 53/126 (42.06%), Postives = 79/126 (62.70%), Query Frame = 3 Query: 240 QSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIR 617 Q++ V + ++ NA++Q RRQNE + R+ N+++Q RR NE + S R+ NA+RQ +RRQ+E E+ + R NAERQ RRLNE E+ RR +A+RQ RRRQNE++E+ + R Sbjct: 65 QNENDVQISARRAANAEQQRHRRQNENDVQVSARRAANAEQQRHRRQNENDVQISARRAANAQRQQIRRQSEDVEQRSARLLTNAERQRRRRLNEDNEQRTARRVAHADRQQRRRQNESAEQRSAR 190 HSP 2 Score: 71.2478 bits (173), Expect = 1.545e-10 Identity = 55/152 (36.18%), Postives = 92/152 (60.53%), Query Frame = 3 Query: 186 SNNSDELQINFDGNQQFN--QSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 +N+++ L+ + + QQ + Q++ V + ++ NA++Q RRQNE + R+ N+++Q RR NE + S R+ NAE+Q RRQNE + + R+ NA+RQ +RR +E E+ + R NAERQ RRR NE +E+ R+ ++A+ Sbjct: 22 ANSAERLRSHPNAEQQRHRRQNENDVQISARRAANAEQQRHRRQNENDVQISARRAANAEQQRHRRQNENDVQVSARRAANAEQQRHRRQNENDVQISARRAANAQRQQIRRQSEDVEQRSARLLTNAERQRRRRLNEDNEQRTARRVAHAD 173 HSP 3 Score: 63.5438 bits (153), Expect = 6.115e-8 Identity = 54/131 (41.22%), Postives = 81/131 (61.83%), Query Frame = 3 Query: 240 QSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNA 632 Q++ V + ++ NA RQ RRQ+E E+ R N++RQ RRLNE E+ + R+ +A+RQ RRQNE+ E+ + R N ER+ RR +E E+ ++R NAERQ R+RQNE+ E+ + R+ NA Sbjct: 111 QNENDVQISARRAANAQRQQIRRQSEDVEQRSARLLTNAERQRRRRLNEDNEQRTARRVAHADRQQRRRQNESAEQRSARLLGNVERERRRRQSEDTEQRSVRLLANAERQQRQRQNESVEQRSARRPMNA 241 HSP 4 Score: 62.3882 bits (150), Expect = 1.629e-7 Identity = 54/132 (40.91%), Postives = 80/132 (60.61%), Query Frame = 3 Query: 240 QSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 Q++ V + ++ NA++Q RRQNE + R+ N+QRQ RR +E E+ S R NAERQ RR NE E+ R+ +A+RQ RR NE+ E+ + R N ER+ RRRQ+E +E+ ++R +NAE Sbjct: 88 QNENDVQVSARRAANAEQQRHRRQNENDVQISARRAANAQRQQIRRQSEDVEQRSARLLTNAERQRRRRLNEDNEQRTARRVAHADRQQRRRQNESAEQRSARLLGNVERERRRRQSEDTEQRSVRLLANAE 219 HSP 5 Score: 57.3806 bits (137), Expect = 9.464e-6 Identity = 49/119 (41.18%), Postives = 74/119 (62.18%), Query Frame = 3 Query: 270 KKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRS 626 +++ +ADRQ +RRQNE++E+ R N +R+ RR +E E+ SVR NAERQ +RQNE+ E+ + R+ +NA RQ RR NE + R R +A R +RRR N+ ++ + RS Sbjct: 167 RRVAHADRQQRRRQNESAEQRSARLLGNVERERRRRQSEDTEQRSVRLLANAERQQRQRQNESVEQRSARRPMNAARQQRRRQNEGVAQRNERLRNDAVRHYRRR-NDAADTTGLALRS 284
BLAST of SMED30011056 vs. TrEMBL
Match: A0A0L7LHT3 (RING-type domain-containing protein OS=Operophtera brumata OX=104452 GN=OBRU01_06817 PE=4 SV=1) HSP 1 Score: 72.0182 bits (175), Expect = 9.862e-11 Identity = 48/121 (39.67%), Postives = 74/121 (61.16%), Query Frame = 3 Query: 270 KKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNA 632 ++ LNA+R +R +ET+EE VR++ + R +E EEA+ R++ NAER + +ET EE A+R++ A R +E PEE A RR+ NAER +R +ET+EE A+R++ A Sbjct: 193 RRQLNAERMDSQRASETAEETTVRRQQLAACMASHRASEVTEEAASRRQENAERMASQGASETAEETAVRRQQVAACMASHRASEVPEEVASRRQENAERMASQRASETAEETAVRRQQVA 313 HSP 2 Score: 70.0922 bits (170), Expect = 4.745e-10 Identity = 51/132 (38.64%), Postives = 78/132 (59.09%), Query Frame = 3 Query: 240 QSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 Q + SV +++ A+RQ+ R ETS + + R++LN++R +R +ET EE +VR++ A R +E EEAA R++ NAER + +ET EETA+RR+ A R +E EE A R++ NAE Sbjct: 160 QQESSVHAEQRRIEQAERQANVRAAETSTQADARRQLNAERMDSQRASETAEETTVRRQQLAACMASHRASEVTEEAASRRQENAERMASQGASETAEETAVRRQQVAACMASHRASEVPEEVASRRQENAE 291 HSP 3 Score: 59.3066 bits (142), Expect = 1.770e-6 Identity = 41/102 (40.20%), Postives = 60/102 (58.82%), Query Frame = 3 Query: 282 NADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQ 587 NA+R + + +ET+EE VR++ + R +E PEE + R++ NAER +R +ET EE A+R++ A +R +ET EE RR AERQ RRQ Sbjct: 243 NAERMASQGASETAEETAVRRQQVAACMASHRASEVPEEVASRRQENAERMASQRASETAEETAVRRQQVAACMASQRASETFEEADTRRHAVAERQAERRQ 344
BLAST of SMED30011056 vs. TrEMBL
Match: A0A2G9UDE8 (Uncharacterized protein OS=Teladorsagia circumcincta OX=45464 GN=TELCIR_09942 PE=4 SV=1) HSP 1 Score: 67.781 bits (164), Expect = 1.192e-10 Identity = 57/113 (50.44%), Postives = 66/113 (58.41%), Query Frame = 3 Query: 276 MLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAI 614 M +A RQ RR ET EE R N+ RQ RR ET EE +VR +A RQ RR ETPEE IR NA RQ RRL ETPEE ++R +A RQ +RR ETSEE + Sbjct: 1 MKDALRQRSRRLEETLEEQTARLEKNAARQRQRRSAETAEERAVRLAEDAARQRKRRLLETPEERCIRLAGNAARQRWRRLLETPEEQSVRLAESAVRQRQRRGPETSEEEPV 113
BLAST of SMED30011056 vs. TrEMBL
Match: A0A0C2GQ68 (Helitron_like_N domain-containing protein OS=Ancylostoma duodenale OX=51022 GN=ANCDUO_08493 PE=4 SV=1) HSP 1 Score: 71.633 bits (174), Expect = 1.393e-10 Identity = 55/121 (45.45%), Postives = 74/121 (61.16%), Query Frame = 3 Query: 270 KKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNA 632 +++ NA+RQ +RRQNE EE R N++RQ RR N EE S R NAERQ RQ+E ++ + R R NAERQ RR NE ++ RR N ERQ R RQNE ++ ++R++ NA Sbjct: 92 RRLANAERQQRRRQNENIEERSARLAANAERQQRRRQNGDFEERSARLAANAERQQRLRQSEDVQQRSERLRANAERQRERRQNEDADQRIARRPANVERQQRLRQNEDADSISMRRQVNA 212 HSP 2 Score: 70.8626 bits (172), Expect = 2.494e-10 Identity = 56/118 (47.46%), Postives = 74/118 (62.71%), Query Frame = 3 Query: 282 NADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 +A R + R+ ET++E VR N+++Q RRL E+ E+AS R+ NAERQ RRQNE EE + R NAERQ RR N EE + R NAERQ R RQ+E ++ + R R+NAE Sbjct: 50 DAQRVRRHRERETAQERNVRVNSNAEQQRRRRLGESTEQASARRLANAERQQRRRQNENIEERSARLAANAERQQRRRQNGDFEERSARLAANAERQQRLRQSEDVQQRSERLRANAE 167 HSP 3 Score: 68.1662 bits (165), Expect = 2.135e-9 Identity = 53/118 (44.92%), Postives = 72/118 (61.02%), Query Frame = 3 Query: 282 NADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 NA++Q +RR E++E+ R+ N++RQ RR NE EE S R NAERQ RRQN EE + R NAERQ R +E ++ + R R NAERQ RRQNE +++ R+ +N E Sbjct: 73 NAEQQRRRRLGESTEQASARRLANAERQQRRRQNENIEERSARLAANAERQQRRRQNGDFEERSARLAANAERQQRLRQSEDVQQRSERLRANAERQRERRQNEDADQRIARRPANVE 190 HSP 4 Score: 62.7734 bits (151), Expect = 1.626e-7 Identity = 51/118 (43.22%), Postives = 71/118 (60.17%), Query Frame = 3 Query: 282 NADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 NA+RQ +RRQN EE R N++RQ R +E ++ S R + NAERQ RRQNE ++ R+ N ERQ R NE + ++RR++NA RQ RQNE ++ A R R++AE Sbjct: 119 NAERQQRRRQNGDFEERSARLAANAERQQRLRQSEDVQQRSERLRANAERQRERRQNEDADQRIARRPANVERQQRLRQNEDADSISMRRQVNAARQRLLRQNEEDDQRAQRLRNDAE 236
BLAST of SMED30011056 vs. TrEMBL
Match: B0WPV7 (Uncharacterized protein OS=Culex quinquefasciatus OX=7176 GN=6041490 PE=4 SV=1) HSP 1 Score: 69.707 bits (169), Expect = 4.083e-10 Identity = 60/152 (39.47%), Postives = 83/152 (54.61%), Query Frame = 3 Query: 180 DISNNSDELQINFDGNQQFNQSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAE 635 D +N+S + + +S+ + A+ + K NA+R ++R NET EE ++R N++ RR NET E ++R NA RQ LRR ETPE+ AIRK A+R R NETPE+ +R A R + ETSEE A R R AE Sbjct: 69 DATNDSSGTSLTKAQRRLLGESEAARAKRLAK--NAERMRQKRSNETYEEYKIRLAKNAEANRRRRANETEAERALRHMRNAMRQRLRRAMETPEQKAIRKARLAQRMREIRANETPEQRKVRLEKGAARARHKFLTETSEERAERNRKKAE 218
BLAST of SMED30011056 vs. Planmine SMEST
Match: SMESG000009090.1 (SMESG000009090.1) HSP 1 Score: 355.14 bits (910), Expect = 6.906e-126 Identity = 195/195 (100.00%), Postives = 195/195 (100.00%), Query Frame = 3 Query: 114 MNNYAGQSFSSDLDNCLKTGSNDISNNSDELQINFDGNQQFNQSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAEXXXXXXXXXXXXXXKCSVPQP 698 MNNYAGQSFSSDLDNCLKTGSNDISNNSDELQINFDGNQQFNQSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAERQLLRRQSQNSRNLKCSVPQP Sbjct: 1 MNNYAGQSFSSDLDNCLKTGSNDISNNSDELQINFDGNQQFNQSDKSVAQAIKKMLNADRQSKRRQNETSEECEVRKRLNSQRQYFRRLNETPEEASVRKKLNAERQWLRRQNETPEEAAIRKRLNAERQYLRRLNETPEETAIRRRLNAERQFRRRQNETSEEAAIRKRSNAERQLLRRQSQNSRNLKCSVPQP 195 The following BLAST results are available for this feature:
BLAST of SMED30011056 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30011056 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30011056 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30011056 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30011056 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30011056 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 1
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011056 ID=SMED30011056|Name=SMED30011056|organism=Schmidtea mediterranea sexual|type=transcript|length=732bpback to top protein sequence of SMED30011056-orf-1 >SMED30011056-orf-1 ID=SMED30011056-orf-1|Name=SMED30011056-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=196bp MNNYAGQSFSSDLDNCLKTGSNDISNNSDELQINFDGNQQFNQSDKSVAQback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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