Protein kinase domain-containing protein
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30011043 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 9
Homology
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: CHEK1 (checkpoint kinase 1 [Source:HGNC Symbol;Acc:HGNC:1925]) HSP 1 Score: 107.457 bits (267), Expect = 3.853e-33 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 3.853e-33 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: CHEK1 (checkpoint kinase 1 [Source:HGNC Symbol;Acc:HGNC:1925]) HSP 1 Score: 107.457 bits (267), Expect = 3.853e-33 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 3.853e-33 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: CHEK1 (checkpoint kinase 1 [Source:HGNC Symbol;Acc:HGNC:1925]) HSP 1 Score: 107.457 bits (267), Expect = 3.853e-33 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 3.853e-33 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: CHEK1 (checkpoint kinase 1 [Source:HGNC Symbol;Acc:HGNC:1925]) HSP 1 Score: 107.457 bits (267), Expect = 3.853e-33 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 3.853e-33 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Match: CHEK1 (checkpoint kinase 1 [Source:HGNC Symbol;Acc:HGNC:1925]) HSP 1 Score: 107.457 bits (267), Expect = 3.853e-33 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 3.853e-33 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: chk-1 (Serine/threonine-protein kinase chk-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9N3Z3]) HSP 1 Score: 109.383 bits (272), Expect = 5.415e-32 Identity = 88/336 (26.19%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW------HDKHWASLTIQRLPVDMINFLIDALAHDPT-GRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQP--------ENVAVIPKSQPTMLKDHLNTSTEVN-----YDFPLSQPINFAELLM-------PSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVD-----SILVDFRRVRGDGLLFHTVFSEV 1464
+++ H +++VH DIKPEN++++G+ V K+ DFG+A + + GT Y+APE Y G D++S + + +TGE W++AS Y W ++ W + ++ L ++ + D T R TI++I+ WY+ G++ T N +P EN+ +++ + + HL T E + + SQP +LL+ +++N +V RMTRF D + T+ +V E H + + + VT +V+ + +Y D ++VDFRR RGDG+ F +F +V
Sbjct: 137 GLKFIHDNDVVHRDIKPENLLLTGTHVLKISDFGMATLYRNKGEERLLDLSCGTIPYAAPELCAGKKYRGPPVDVWSSGIVLIAMLTGELPWDRASDASQSYMGWISNTSLDERPWKKIDVRAL------CMLRKIVTDKTDKRATIEQIQADPWYQHNF-GQVET----PNGRPLKRARNNDENITCTQQAECSAKRRHLETPNEKSTLAERQNASFSQPTKTEDLLLTQHIDMSQTNSNLLQRMVCRMTRFCVVTDIRSTYQKVARASE-HAGFGVRETDDYRLLVTWR----EVSMMVSLYTMGDIPDKPRVMVDFRRSRGDGIQFKKMFMDV 456 HSP 2 Score: 48.1358 bits (113), Expect = 5.415e-32 Identity = 33/111 (29.73%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAV--KIVRSMINEKLRKRIREEFRIQKNLF---HRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
Y + +GEGA G V N +N + A KI + ++ IR+E+ +QK + H N++ + G+ N F +F+E G+LFD I CG++ + +KQ+
Sbjct: 24 YRVVQTLGEGAFGEVLLIVNTKNPEVAAAMKKINIANKSKDFIDNIRKEYLLQKRVSAVGHDNVIRMIGMRNDPQFYYLFLEYADGGELFDKIEPDCGMSPVFAQFYFKQL 134
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: chk-1 (Serine/threonine-protein kinase chk-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9N3Z3]) HSP 1 Score: 108.997 bits (271), Expect = 1.419e-25 Identity = 88/336 (26.19%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW------HDKHWASLTIQRLPVDMINFLIDALAHDPT-GRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQP--------ENVAVIPKSQPTMLKDHLNTSTEVN-----YDFPLSQPINFAELLM-------PSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVD-----SILVDFRRVRGDGLLFHTVFSEV 1464
+++ H +++VH DIKPEN++++G+ V K+ DFG+A + + GT Y+APE Y G D++S + + +TGE W++AS Y W ++ W + ++ L ++ + D T R TI++I+ WY+ G++ T N +P EN+ +++ + + HL T E + + SQP +LL+ +++N +V RMTRF D + T+ +V E H + + + VT +V+ + +Y D ++VDFRR RGDG+ F +F +V
Sbjct: 45 GLKFIHDNDVVHRDIKPENLLLTGTHVLKISDFGMATLYRNKGEERLLDLSCGTIPYAAPELCAGKKYRGPPVDVWSSGIVLIAMLTGELPWDRASDASQSYMGWISNTSLDERPWKKIDVRAL------CMLRKIVTDKTDKRATIEQIQADPWYQHNF-GQVET----PNGRPLKRARNNDENITCTQQAECSAKRRHLETPNEKSTLAERQNASFSQPTKTEDLLLTQHIDMSQTNSNLLQRMVCRMTRFCVVTDIRSTYQKVARASE-HAGFGVRETDDYRLLVTWR----EVSMMVSLYTMGDIPDKPRVMVDFRRSRGDGIQFKKMFMDV 364 HSP 2 Score: 26.5646 bits (57), Expect = 1.419e-25 Identity = 13/40 (32.50%), Postives = 21/40 (52.50%), Query Frame = 2
Query: 443 NTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
N F +F+E G+LFD I CG++ + +KQ+
Sbjct: 3 NDPQFYYLFLEYADGGELFDKIEPDCGMSPVFAQFYFKQL 42
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: chk-1 (Serine/threonine-protein kinase chk-1 [Source:UniProtKB/Swiss-Prot;Acc:Q9N3Z3]) HSP 1 Score: 108.997 bits (271), Expect = 1.419e-25 Identity = 88/336 (26.19%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW------HDKHWASLTIQRLPVDMINFLIDALAHDPT-GRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQP--------ENVAVIPKSQPTMLKDHLNTSTEVN-----YDFPLSQPINFAELLM-------PSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVD-----SILVDFRRVRGDGLLFHTVFSEV 1464
+++ H +++VH DIKPEN++++G+ V K+ DFG+A + + GT Y+APE Y G D++S + + +TGE W++AS Y W ++ W + ++ L ++ + D T R TI++I+ WY+ G++ T N +P EN+ +++ + + HL T E + + SQP +LL+ +++N +V RMTRF D + T+ +V E H + + + VT +V+ + +Y D ++VDFRR RGDG+ F +F +V
Sbjct: 45 GLKFIHDNDVVHRDIKPENLLLTGTHVLKISDFGMATLYRNKGEERLLDLSCGTIPYAAPELCAGKKYRGPPVDVWSSGIVLIAMLTGELPWDRASDASQSYMGWISNTSLDERPWKKIDVRAL------CMLRKIVTDKTDKRATIEQIQADPWYQHNF-GQVET----PNGRPLKRARNNDENITCTQQAECSAKRRHLETPNEKSTLAERQNASFSQPTKTEDLLLTQHIDMSQTNSNLLQRMVCRMTRFCVVTDIRSTYQKVARASE-HAGFGVRETDDYRLLVTWR----EVSMMVSLYTMGDIPDKPRVMVDFRRSRGDGIQFKKMFMDV 364 HSP 2 Score: 26.5646 bits (57), Expect = 1.419e-25 Identity = 13/40 (32.50%), Postives = 21/40 (52.50%), Query Frame = 2
Query: 443 NTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
N F +F+E G+LFD I CG++ + +KQ+
Sbjct: 3 NDPQFYYLFLEYADGGELFDKIEPDCGMSPVFAQFYFKQL 42
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: Y43D4A.6 (pep chromosome:WBcel235:IV:16787327:16788510:1 gene:WBGene00012792.1 transcript:Y43D4A.6.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:Y43D4A.6) HSP 1 Score: 75.0998 bits (183), Expect = 1.722e-25 Identity = 47/151 (31.13%), Postives = 75/151 (49.67%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLA----VDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW-HDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIW 996
+ ++Y H IVH DIKP+N++I+ S K+ DFGL+ VD I + R+GTT + APE F + G D++S + + +TG W++ D Y W ++K + L ++ FL L + R TI+ I+ W
Sbjct: 115 SGLKYIHGCGIVHRDIKPDNLLINSSDALKIADFGLSTRFIVDDKEI-LLHERIGTTPFMAPELFAKKKHRGPPVDIWSAGILVFSLLTGNLPWDEPKESDILYSQWMNNKTSKNCIWYGLSTPVVRFLKKLLKENVNERATIEEIESDPW 264 HSP 2 Score: 60.4622 bits (145), Expect = 1.722e-25 Identity = 39/119 (32.77%), Postives = 67/119 (56.30%), Query Frame = 2
Query: 239 NSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMIN-----EKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPI--GCGINTLDGFKIYKQMQSSI 574
N ++ IG+GA G V+ A+ N + +K+ IN ++ ++I++E+ IQKN+ H+NIV++YG+ F +F E +T GDLF+ I G +N + G+ KQ+ S +
Sbjct: 4 NQFKPIKSIGKGAFGQVFLAEKLNNPR---IKVAMKKINIFGAPKEFLEQIQQEYYIQKNMKHKNIVKMYGMSMDTEFCYLFQEFVTGGDLFEKIDFENGMPVNQVKGY--LKQLMSGL 117
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Match: kin-34 (pep chromosome:WBcel235:V:244469:245846:-1 gene:WBGene00019815.1 transcript:R02C2.2.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:kin-34) HSP 1 Score: 71.2478 bits (173), Expect = 3.251e-22 Identity = 45/155 (29.03%), Postives = 68/155 (43.87%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPT---IKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASL-TIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
N +++ H ++ H DIKPEN+M++ SG K+ DFGLA + + R G+ Y APE + Y G + D++S V L +T + W + Y W A + L L L+ R TI+ I+ WY G
Sbjct: 134 VNGLKFLHCRDVAHRDIKPENLMLTHSGHLKIADFGLATWYRYKGAELIFEQRCGSVEYVAPEIYTGAQYRGPQIDIWSAGVVLLAMLTRQLPWEFPCMTNKRYFFWMKNRVAHIDAWNELSCRAKKLLWKMLSPGSENRATIEEIQSSAWYLFG 288 HSP 2 Score: 53.1434 bits (126), Expect = 3.251e-22 Identity = 34/116 (29.31%), Postives = 59/116 (50.86%), Query Frame = 2
Query: 224 EPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAV--KIVRSMINEKLRKRIREEFRIQKNLF-HRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
P P YE+ +G+G+ G V N+ + LA +IV + + + IR+E IQ++L H N+++V G + F +F+E GDL++ I GC + + +KQ+
Sbjct: 20 HPDRP--YEVLKPLGQGSFGQVALVSNRRYPEMLAALKRIVITGQDREYIDAIRKEITIQESLTGHENVIQVLGKESDAQFCYMFLEYADGGDLYEKITSGCSFSLEEAHSYFKQL 133
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: grp (gene:FBgn0261278 transcript:FBtr0080886) HSP 1 Score: 89.7373 bits (221), Expect = 1.096e-26 Identity = 91/360 (25.28%), Postives = 141/360 (39.17%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPT---IKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDK-HWASLT-IQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIG-------------------KISTVKIQTNFQPEN-------VAVIPKSQPTMLK------------------DHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSS--------IVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSA-VSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
+ + Y H+ I H D+KPEN+++ K+ DFG+A + R GT Y APE L Y ADL+S V +T + GE W++ S + +W D HW T +L I+ L LA P RLT+++ DH W K ++Q++ N + PTM D + E + SQP +LL+ + N+T + +V+RMTRFF T +T R+ IE+ T + + V++ + + F+ I + ILVD R +G GL F F ++
Sbjct: 129 SGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEV-LQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKI 487 HSP 2 Score: 50.447 bits (119), Expect = 1.096e-26 Identity = 31/110 (28.18%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
+ ++ +GEGA G V N++ G+ +A+K+V + +R+E IQK L ++I+ +G + S IF+E G+LFD I G+ + + + Q+ S +
Sbjct: 22 WTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGL 131
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: grp (gene:FBgn0261278 transcript:FBtr0080885) HSP 1 Score: 89.7373 bits (221), Expect = 1.096e-26 Identity = 91/360 (25.28%), Postives = 141/360 (39.17%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPT---IKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDK-HWASLT-IQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIG-------------------KISTVKIQTNFQPEN-------VAVIPKSQPTMLK------------------DHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSS--------IVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSA-VSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
+ + Y H+ I H D+KPEN+++ K+ DFG+A + R GT Y APE L Y ADL+S V +T + GE W++ S + +W D HW T +L I+ L LA P RLT+++ DH W K ++Q++ N + PTM D + E + SQP +LL+ + N+T + +V+RMTRFF T +T R+ IE+ T + + V++ + + F+ I + ILVD R +G GL F F ++
Sbjct: 129 SGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEV-LQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKI 487 HSP 2 Score: 50.447 bits (119), Expect = 1.096e-26 Identity = 31/110 (28.18%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
+ ++ +GEGA G V N++ G+ +A+K+V + +R+E IQK L ++I+ +G + S IF+E G+LFD I G+ + + + Q+ S +
Sbjct: 22 WTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGL 131
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: grp (gene:FBgn0261278 transcript:FBtr0080884) HSP 1 Score: 89.7373 bits (221), Expect = 1.096e-26 Identity = 91/360 (25.28%), Postives = 141/360 (39.17%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPT---IKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDK-HWASLT-IQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIG-------------------KISTVKIQTNFQPEN-------VAVIPKSQPTMLK------------------DHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSS--------IVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSA-VSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
+ + Y H+ I H D+KPEN+++ K+ DFG+A + R GT Y APE L Y ADL+S V +T + GE W++ S + +W D HW T +L I+ L LA P RLT+++ DH W K ++Q++ N + PTM D + E + SQP +LL+ + N+T + +V+RMTRFF T +T R+ IE+ T + + V++ + + F+ I + ILVD R +G GL F F ++
Sbjct: 129 SGLNYLHQRGIAHRDLKPENLLLDEHDNVKISDFGMATMFRCKGKERLLDKRCGTLPYVAPEV-LQKAYHAQPADLWSCGVILVTMLAGELPWDQPSTNCTEFTNWRDNDHWQLQTPWSKLDTLAISLLRKLLATSPGTRLTLEKTLDHKWCNMQFADNERSYDLVDSAAALEICSPKAKRQRLQSSAHLSNGLDDSISRNYCSQPMPTMRSDDDFNVRLGSGRSKEDGGDRQTLAQEARLSYSFSQPALLDDLLLATQMNQTQNASQNYFQRLVRRMTRFFVTTRWDDTIKRLVGTIERLGGYTCKFGDDGVVTVSTVDRNKLRLVFKAHIIEMDGKILVDCRLSKGCGLEFKRRFIKI 487 HSP 2 Score: 50.447 bits (119), Expect = 1.096e-26 Identity = 31/110 (28.18%), Postives = 57/110 (51.82%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
+ ++ +GEGA G V N++ G+ +A+K+V + +R+E IQK L ++I+ +G + S IF+E G+LFD I G+ + + + Q+ S +
Sbjct: 22 WTLAQTLGEGAYGEVKLLINRQTGEAVAMKMVDLKKHPDAANSVRKEVCIQKMLQDKHILRFFGKRSQGSVEYIFLEYAAGGELFDRIEPDVGMPQHEAQRYFTQLLSGL 131
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: Mekk1 (gene:FBgn0024329 transcript:FBtr0083684) HSP 1 Score: 73.9442 bits (180), Expect = 2.687e-22 Identity = 53/159 (33.33%), Postives = 82/159 (51.57%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENV-MISGSGVPKLIDFGLAVD---HSVIP-TIKGRLGTTAYSAPETF--LDCDYTGFKADLFSLIVTFLTAVTGERLWNK--ASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
+ V HKH IVH DIK N+ ++ GS KL DFG AV H+ +P ++G +GT AY APE F + D G AD++S+ + +G+R W + ++F+ +K A ++ + D I+ L HDP RLT + +H + + G
Sbjct: 1390 SGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGELQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVGMGEKPQAPESLSQEGHDFIDH---CLQHDPKRRLTAVELLEHNFCKYG 1545 HSP 2 Score: 51.2174 bits (121), Expect = 2.687e-22 Identity = 29/85 (34.12%), Postives = 46/85 (54.12%), Query Frame = 2
Query: 263 IGEGAIGVVYTAQNKENGKFLAVKIVRSMINE-KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPI 514
IG+G G VYTA N G+ +A+K + E + K + EE +I + + H+N+V YGI + IFME + G L ++ +
Sbjct: 1288 IGQGRFGKVYTAVNNNTGELMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVEL 1372
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Match: Mekk1 (gene:FBgn0024329 transcript:FBtr0331353) HSP 1 Score: 73.559 bits (179), Expect = 3.170e-22 Identity = 53/159 (33.33%), Postives = 82/159 (51.57%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENV-MISGSGVPKLIDFGLAVD---HSVIP-TIKGRLGTTAYSAPETF--LDCDYTGFKADLFSLIVTFLTAVTGERLWNK--ASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
+ V HKH IVH DIK N+ ++ GS KL DFG AV H+ +P ++G +GT AY APE F + D G AD++S+ + +G+R W + ++F+ +K A ++ + D I+ L HDP RLT + +H + + G
Sbjct: 1297 SGVSELHKHGIVHRDIKTANIFLVDGSNSLKLGDFGSAVKIQAHTTVPGELQGYVGTQAYMAPEVFTKTNSDGHGRAADIWSVGCVVVEMASGKRPWAQFDSNFQIMFKVGMGEKPQAPESLSQEGHDFIDH---CLQHDPKRRLTAVELLEHNFCKYG 1452 HSP 2 Score: 51.2174 bits (121), Expect = 3.170e-22 Identity = 29/85 (34.12%), Postives = 46/85 (54.12%), Query Frame = 2
Query: 263 IGEGAIGVVYTAQNKENGKFLAVKIVRSMINE-KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPI 514
IG+G G VYTA N G+ +A+K + E + K + EE +I + + H+N+V YGI + IFME + G L ++ +
Sbjct: 1195 IGQGRFGKVYTAVNNNTGELMAMKEIAIQPGETRALKNVAEELKILEGIKHKNLVRYYGIEVHREELLIFMELCSEGTLESLVEL 1279
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: chek1 (checkpoint kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-896]) HSP 1 Score: 105.916 bits (263), Expect = 2.618e-24 Identity = 97/349 (27.79%), Postives = 149/349 (42.69%), Query Frame = 1
Query: 571 YCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCH-YCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWY---------RQGLIGKISTVKIQTNFQPENV---AVIPKSQ--PTMLKDHLNTSTEVNYDFPLSQPINFAELLM--------PSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDV--NFRIFIYKFV---DSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLI 1524
+ H I H DIKPEN+++ K+ DFGLA H RL GT Y APE + AD ++ + + GE W++ S E+C Y DW ++ +++ ++ L L H+P R TI IK H W+ RQG+ + + + +N A I SQ P L + + + SQP +L+ +S + +V+RMTRFF+T + + + +++ H N + VS D N IFI F+ + ILVDFR +GDGL F +F K+ ++L + N L LI
Sbjct: 71 FGHSVGITHRDIKPENILLDDKDNLKISDFGLATMFRHRGRERALNRLCGTLPYVAPELMSRSSFNAQPADTWACGIVLTAMLAGELPWDQPS-ENCQEYLDWLERKTYLTPWKKIDAVPLSLLSKILLHNPEDRFTIPEIKKHRWFSRSFKSAVQRQGITPAAKIQRADSELRRKNSDDRAQISSSQPEPQGLWEEKEVTVHTDVSVSFSQPTCPDHMLLGTQLLGTPGASQSPWQRLVRRMTRFFTTENTEASCIALRDSCIALGHTWKQSCNNQ-----ATVSTMDRRNNKLIFIVHFLEMEERILVDFRLSKGDGLEFKRIF---LKLKQKLSDIISNQKVLPLI 410
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: snrka (SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040718-84]) HSP 1 Score: 74.3294 bits (181), Expect = 2.328e-21 Identity = 45/149 (30.20%), Postives = 73/149 (48.99%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYR 1002
+A+ YCH+ ++VH D+KPENV+ G+ KL DFG + + G+ AYSAPE L +Y D++SL V V G+ + +A+ + K+ + D+IN + L DP R +++ I+ H W +
Sbjct: 125 HAISYCHRLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVCGQPPFQEANDSETLTMIMDCKYTVPAHVSNACKDLINRM---LQRDPKRRASLEEIESHAWLQ 270 HSP 2 Score: 47.7506 bits (112), Expect = 2.328e-21 Identity = 29/104 (27.88%), Postives = 56/104 (53.85%), Query Frame = 2
Query: 200 MQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
M +K + K+ Y++ +G G VV A++ G+ +AVK++ ++ ++ + +E R K + H NIV +Y +I+T + + + +E GD+FD I
Sbjct: 1 MAGIKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTVATGHLFQEVRCMKLVQHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYI 104
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: snrka (SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040718-84]) HSP 1 Score: 74.7146 bits (182), Expect = 6.033e-21 Identity = 47/151 (31.13%), Postives = 74/151 (49.01%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL 1011
A+ YCH+ ++VH D+KPENV+ G+ KL DFG + + G+ AYSAPE L +Y D++SL V V G+ + +A+ + K+ + D+IN + L DP R +++ I+ H W QG+
Sbjct: 126 AISYCHRLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVCGQPPFQEANDSETLTMIMDCKYTVPAHVSNACKDLINRM---LQRDPKRRASLEEIESHAWL-QGV 272 HSP 2 Score: 46.2098 bits (108), Expect = 6.033e-21 Identity = 29/104 (27.88%), Postives = 56/104 (53.85%), Query Frame = 2
Query: 200 MQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
M +K + K+ Y++ +G G VV A++ G+ +AVK++ ++ ++ + +E R K + H NIV +Y +I+T + + + +E GD+FD I
Sbjct: 1 MAGIKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTVATGHLFQEVRCMKLVQHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYI 104
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: sik1 (salt-inducible kinase 1 [Source:ZFIN;Acc:ZDB-GENE-030131-9446]) HSP 1 Score: 74.7146 bits (182), Expect = 7.409e-21 Identity = 47/149 (31.54%), Postives = 71/149 (47.65%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPV----DMINFLIDALAHDPTGRLTIDRIKDHIW 996
AV+YCH+H+IVH D+K EN+++ + KL DFG ++ + G+ Y+APE F +Y G + D++SL V V G ++ S + R+P D N + L DP R+TI +IK H W
Sbjct: 135 AVDYCHRHHIVHRDLKTENLLLDANMNIKLADFGFGNFYNAGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGDSLPALRQRVTEGRF-------RIPFFMSQDCENLIRKMLVVDPAKRITIAQIKQHRW 276 HSP 2 Score: 45.8246 bits (107), Expect = 7.409e-21 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
YEI +G+G VV A++K +A+KI+ ++ +N ++I E +I K L H +I+++Y ++ T + I E G++FD +
Sbjct: 26 YEIIRTLGKGNFAVVKLARHKVTKTQVAIKIIDKTRLNSANLEKIYREVQIMKLLNHPHIIKLYQVMETKDMLYIVTEYAKNGEMFDYL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Match: mark3a (MAP/microtubule affinity-regulating kinase 3a [Source:ZFIN;Acc:ZDB-GENE-030131-6232]) HSP 1 Score: 73.559 bits (179), Expect = 1.785e-20 Identity = 46/154 (29.87%), Postives = 74/154 (48.05%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
+AV+YCH+ IVH D+K EN+++ G K+ DFG + + V + G+ Y+APE F Y G + D++SL V T V+G ++ + ++ K+ R+P D N L L +P R T+++I W G
Sbjct: 165 SAVQYCHQKRIVHRDLKAENLLLDGDMNIKIADFGFSNEFMVGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY-------RIPFYMSTDCENLLKRFLVLNPAKRGTLEQIMKERWINSG 311 HSP 2 Score: 45.8246 bits (107), Expect = 1.785e-20 Identity = 28/98 (28.57%), Postives = 55/98 (56.12%), Query Frame = 2
Query: 218 EIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
++ P + N Y + IG+G V A++ G +A+K++ ++ +N +++ E I KNL H NIV+++ +I T + + ME + G++FD +
Sbjct: 49 DVHPVIGN-YRLLKTIGKGNFAKVKLARHILTGSEVAIKMIDKTQLNPTSLQKLSREVTIMKNLNHPNIVKLFEVIETEKTLFLVMEYASGGEVFDYL 145
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: bnc2 (basonuclin 2 [Source:Xenbase;Acc:XB-GENE-953157]) HSP 1 Score: 108.612 bits (270), Expect = 2.810e-33 Identity = 86/343 (25.07%), Postives = 147/343 (42.86%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQ--------------------GLIGKIST---VKIQTNFQPENVAVIPKSQP-TMLKDHLNTSTEVN-----YDFPLSQPINFAELLMP--------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFK 1470
VEY H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE + D++S + + GE W++ + YCDW +K+ +++ + L L +P R+TI IK W+ + GL +I + + I ++ + +N + +P T L + + ++ SQP +L+ SS N +VKRMTRFF+ +DA+ ++ + + E+ + N T+ + F++ + + D IL+DFR +GDGL F F ++ K
Sbjct: 117 GVEYLHSIGITHRDIKPENLLLDDRDHLKISDFGLATVFRHNAKERLLNKMCGTLPYVAPELIKSRAFNAEPVDVWSCGIVLTAMLAGELPWDQPTEACQEYCDWKEKNHYLTPWKKIGATPLALLCKMLTENPQSRITIPDIKKDRWFTELIKKGIKRSRAFSGGTSDSSGLCKQIRSDMEISIISHSEEKNAYSSTQPEPRTALATWDSNGSHIDNLVQGKGISFSQPACPENMLLSSQLIGTPGSSQNVWQRLVKRMTRFFTKVDAESSYSNLMDTCEKMGYVLKKSCANQVTLSTTDRRNNKLIFKVNLVEMEDRILLDFRLSKGDGLEFKRHFLKIKK 459 HSP 2 Score: 54.6842 bits (130), Expect = 2.810e-33 Identity = 34/107 (31.78%), Postives = 54/107 (50.47%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEGA G V A N++ + +AVKIV + I++E I + L H NIV YG + +F+E G+LFD I G+ D K ++Q+
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRKTEEAVAVKIVDMTRAADCPENIKKEICINRMLNHTNIVRFYGHRREGNIQYLFLEYCRGGELFDRIEPDVGMPEQDAQKFFQQL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: bnc2 (basonuclin 2 [Source:Xenbase;Acc:XB-GENE-953157]) HSP 1 Score: 108.227 bits (269), Expect = 4.901e-25 Identity = 86/343 (25.07%), Postives = 147/343 (42.86%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQ--------------------GLIGKIST---VKIQTNFQPENVAVIPKSQP-TMLKDHLNTSTEVN-----YDFPLSQPINFAELLMP--------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFK 1470
VEY H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE + D++S + + GE W++ + YCDW +K+ +++ + L L +P R+TI IK W+ + GL +I + + I ++ + +N + +P T L + + ++ SQP +L+ SS N +VKRMTRFF+ +DA+ ++ + + E+ + N T+ + F++ + + D IL+DFR +GDGL F F ++ K
Sbjct: 38 GVEYLHSIGITHRDIKPENLLLDDRDHLKISDFGLATVFRHNAKERLLNKMCGTLPYVAPELIKSRAFNAEPVDVWSCGIVLTAMLAGELPWDQPTEACQEYCDWKEKNHYLTPWKKIGATPLALLCKMLTENPQSRITIPDIKKDRWFTELIKKGIKRSRAFSGGTSDSSGLCKQIRSDMEISIISHSEEKNAYSSTQPEPRTALATWDSNGSHIDNLVQGKGISFSQPACPENMLLSSQLIGTPGSSQNVWQRLVKRMTRFFTKVDAESSYSNLMDTCEKMGYVLKKSCANQVTLSTTDRRNNKLIFKVNLVEMEDRILLDFRLSKGDGLEFKRHFLKIKK 380
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: bnc2 (basonuclin 2 [Source:Xenbase;Acc:XB-GENE-953157]) HSP 1 Score: 88.9669 bits (219), Expect = 2.499e-24 Identity = 78/312 (25.00%), Postives = 129/312 (41.35%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNE-TSSIVKRMTRFFSTLDAKETFDRVKNII-----EQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFK 1470
VEY H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE + D++S + + GE W++ + YCDW +K+ +++ + L L +P R+TI IK W+ + + I + + ++ + + + M ++ S E N + +QP L SN ++V+ FS E +I Q T +R N I F++ + + D IL+DFR +GDGL F F ++ K
Sbjct: 111 GVEYLHSIGITHRDIKPENLLLDDRDHLKISDFGLATVFRHNAKERLLNKMCGTLPYVAPELIKSRAFNAEPVDVWSCGIVLTAMLAGELPWDQPTEACQEYCDWKEKNHYLTPWKKIGATPLALLCKMLTENPQSRITIPDIKKDRWFTELIKKGIKRSRAFSGGTSDSSGLCKQIRSDMEISIISHSEEKNA-YSSTQPEPRTALATWDSNGSHIDNLVQGKGISFSQPACPENMLLSSQLIGTPGSSQVTLSTTDRRNNKLI------------FKVNLVEMEDRILLDFRLSKGDGLEFKRHFLKIKK 409 HSP 2 Score: 44.2838 bits (103), Expect = 2.499e-24 Identity = 31/107 (28.97%), Postives = 51/107 (47.66%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEG A N++ + +AVKIV + I++E I + L H NIV YG + +F+E G+LFD I G+ D K ++Q+
Sbjct: 8 DWDLVQTLGEG------LAVNRKTEEAVAVKIVDMTRAADCPENIKKEICINRMLNHTNIVRFYGHRREGNIQYLFLEYCRGGELFDRIEPDVGMPEQDAQKFFQQL 108
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: bnc2 (basonuclin 2 [Source:Xenbase;Acc:XB-GENE-953157]) HSP 1 Score: 86.6557 bits (213), Expect = 1.063e-23 Identity = 70/282 (24.82%), Postives = 120/282 (42.55%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQ--------------------GLIGKIST---VKIQTNFQPENVAVIPKSQP-TMLKDHLNTSTEVN-----YDFPLSQPINFAELLMP--------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQ 1287
VEY H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE + D++S + + GE W++ + YCDW +K+ +++ + L L +P R+TI IK W+ + GL +I + + I ++ + +N + +P T L + + ++ SQP +L+ SS N +VKRMTRFF+ +DA+ ++ + + E+
Sbjct: 111 GVEYLHSIGITHRDIKPENLLLDDRDHLKISDFGLATVFRHNAKERLLNKMCGTLPYVAPELIKSRAFNAEPVDVWSCGIVLTAMLAGELPWDQPTEACQEYCDWKEKNHYLTPWKKIGATPLALLCKMLTENPQSRITIPDIKKDRWFTELIKKGIKRSRAFSGGTSDSSGLCKQIRSDMEISIISHSEEKNAYSSTQPEPRTALATWDSNGSHIDNLVQGKGISFSQPACPENMLLSSQLIGTPGSSQNVWQRLVKRMTRFFTKVDAESSYSNLMDTCEK 392 HSP 2 Score: 44.2838 bits (103), Expect = 1.063e-23 Identity = 31/107 (28.97%), Postives = 51/107 (47.66%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEG A N++ + +AVKIV + I++E I + L H NIV YG + +F+E G+LFD I G+ D K ++Q+
Sbjct: 8 DWDLVQTLGEG------LAVNRKTEEAVAVKIVDMTRAADCPENIKKEICINRMLNHTNIVRFYGHRREGNIQYLFLEYCRGGELFDRIEPDVGMPEQDAQKFFQQL 108
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Match: SIK3 (SIK family kinase 3 [Source:NCBI gene;Acc:100492939]) HSP 1 Score: 71.2478 bits (173), Expect = 8.659e-21 Identity = 51/183 (27.87%), Postives = 86/183 (46.99%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG--------LIGKISTVKIQTNFQPENVAVI 1074
AV +CH NIVH D+K EN+++ + K+ DFG + + +K G+ Y+APE F +Y G K D++SL V V G ++ ++ ++ K R+P + + + L +P+ RL++++I H W QG LI + VK++ P N V+
Sbjct: 122 AVHFCHCRNIVHRDLKAENLLLDANLNIKIADFGFSNRFTPGQLLKTWCGSPPYAAPELFEGKEYDGPKVDIWSLGVVLYVLVCGALPFDGSTLQNLRARVLSGKF-------RIPFFMSTECEHLIRHMLVLEPSKRLSMEQICKHKWMCQGEQDLEFERLIAECQHVKLERQRDPLNEQVL 297 HSP 2 Score: 50.0618 bits (118), Expect = 8.659e-21 Identity = 33/123 (26.83%), Postives = 63/123 (51.22%), Query Frame = 2
Query: 209 LKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
L + P YEI IG+G VV A + +A+KI+ ++ ++E+ K+I E +I K L H +I+ +Y ++ T I + E + G++FD + + + K +KQ+ +++
Sbjct: 1 LAQDAAPARIGYYEIDRTIGKGNFAVVKLATHIVTRAKVAIKIIDKTKLDEENLKKIFREVQIMKMLCHPHIIRLYQVMETERMIYLVTEYASGGEIFDHLVAHGRMAEKEARKKFKQIVAAV 123
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Chek1 (checkpoint kinase 1 [Source:MGI Symbol;Acc:MGI:1202065]) HSP 1 Score: 98.5969 bits (244), Expect = 1.768e-30 Identity = 93/343 (27.11%), Postives = 140/343 (40.82%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVK----------------IQTN--FQP------ENVAVIPKSQPTMLKD-HLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L P+ R+TI IK WY + L + I +N F P E SQP L + N D L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F+I + + + ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVETPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPVNNGSSEETVKFSSSQPEPRTGLSLWDTGPSNVD-KLVQGISFSQPTCPEHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTTDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 54.299 bits (129), Expect = 1.768e-30 Identity = 34/112 (30.36%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLSHENVVKFYGHRREGHIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Chek1 (checkpoint kinase 1 [Source:MGI Symbol;Acc:MGI:1202065]) HSP 1 Score: 98.5969 bits (244), Expect = 1.768e-30 Identity = 93/343 (27.11%), Postives = 140/343 (40.82%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVK----------------IQTN--FQP------ENVAVIPKSQPTMLKD-HLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L P+ R+TI IK WY + L + I +N F P E SQP L + N D L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F+I + + + ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVETPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPVNNGSSEETVKFSSSQPEPRTGLSLWDTGPSNVD-KLVQGISFSQPTCPEHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTTDRRNNKLIFKINLVEMDEKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 54.299 bits (129), Expect = 1.768e-30 Identity = 34/112 (30.36%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLSHENVVKFYGHRREGHIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Chek1 (checkpoint kinase 1 [Source:MGI Symbol;Acc:MGI:1202065]) HSP 1 Score: 80.1073 bits (196), Expect = 5.680e-25 Identity = 77/284 (27.11%), Postives = 114/284 (40.14%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVK----------------IQTN--FQP------ENVAVIPKSQPTMLKD-HLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQ 1287
V Y H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L P+ R+TI IK WY + L + I +N F P E SQP L + N D L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVETPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPVNNGSSEETVKFSSSQPEPRTGLSLWDTGPSNVD-KLVQGISFSQPTCPEHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEK 399 HSP 2 Score: 54.299 bits (129), Expect = 5.680e-25 Identity = 34/112 (30.36%), Postives = 56/112 (50.00%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLSHENVVKFYGHRREGHIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gm5157 (predicted gene 5157 [Source:MGI Symbol;Acc:MGI:3648211]) HSP 1 Score: 88.1965 bits (217), Expect = 1.161e-24 Identity = 64/182 (35.16%), Postives = 90/182 (49.45%), Query Frame = 1
Query: 535 RRI*NL*TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIK--GRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTI-QRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAV 1071
RR+ +AV+YCH H+IVH DIK N++I G KL DFGLA + VIP K G GT Y APE Y G D++SL V V+G + SF D + A+ +I +P+D+ N +I+ L +P+ R TI +I H R G + Q + P+ A+
Sbjct: 124 RRLFAQIVHAVQYCHDHHIVHRDIKASNILIDCRGNAKLCDFGLAAE--VIPGQKLAGFCGTLPYCAPELLQAEKYEGPPVDIWSLGVLLFLMVSGNLPFQGKSFVDLK----QEIISANFSIPSHVPIDIFNVIIELLMINPSRRPTIHQIMRHPMIR-GSEACLPPTSTQISQAPQATAL 298 HSP 2 Score: 45.0542 bits (105), Expect = 1.161e-24 Identity = 33/108 (30.56%), Postives = 56/108 (51.85%), Query Frame = 2
Query: 191 KELMQHLKS--EIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
K + Q LK+ IE +Y++ N +GEG VV A + +AVKI+++ ++ I E RI K+L H NI++++ ++ + ME + G+L D I
Sbjct: 7 KMMEQDLKACYSIEENFDTNYKMLNTLGEGKFSVVKRAFHVPTSTSVAVKILQN--TKEYTSPICREARIMKSLSHPNIIKLFHVVQRRETTYLVMEYASEGELQDRI 112
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Match: Gm5890 (predicted gene 5890 [Source:MGI Symbol;Acc:MGI:3649012]) HSP 1 Score: 85.5001 bits (210), Expect = 2.755e-24 Identity = 58/152 (38.16%), Postives = 80/152 (52.63%), Query Frame = 1
Query: 535 RRI*NL*TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIK--GRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTI-QRLPVDMINFLIDALAHDPTGRLTIDRI 981
RR+ +AV+YCH H+IVH DIK N++I G KL DFGLA + VIP K G GT Y APE Y G D++SL V V+G + SF D + A+ +I +P+D+ N +I+ L +P+ R TI +I
Sbjct: 123 RRLFAQIVHAVQYCHDHHIVHRDIKASNILIDCRGNAKLCDFGLAAE--VIPGQKLAGFCGTLPYCAPELLQAEKYEGPPVDIWSLGVLLFLMVSGNLPFQGKSFVDLK----QEIISANFSIPSHVPIDIFNVIIELLMINPSRRPTIHQI 268 HSP 2 Score: 46.595 bits (109), Expect = 2.755e-24 Identity = 33/108 (30.56%), Postives = 56/108 (51.85%), Query Frame = 2
Query: 191 KELMQHLKS--EIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
K + Q LK+ IE +Y++ N +GEG VV A + +AVKI+++ ++ I E RI K+L H NI++++ ++ + ME + G+L D I
Sbjct: 6 KMMEQDLKACYSIEENFDTNYKMLNTLGEGNFSVVKRAFHVPTSTSVAVKILQN--TKEYTSPICREARIMKSLSHPNIIKLFHVVQRRETTYLVMEYASEGELLDCI 111
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|O14757|CHK1_HUMAN (Serine/threonine-protein kinase Chk1 OS=Homo sapiens OX=9606 GN=CHEK1 PE=1 SV=2) HSP 1 Score: 107.457 bits (267), Expect = 1.694e-32 Identity = 95/342 (27.78%), Postives = 144/342 (42.11%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL----------IGKIS------TVKIQTN--FQPENVAVIPK------SQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA ++ + GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L G +S + IQ+N F P N A + SQP T +Y L Q I+F++ P SS N +VKRMTRFF+ LDA +++ +K E+ + N T+ + F++ + + D ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRYNNRERLLNKMCGTLPYVAPELLKRREFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLKKGAKRPRVTSGGVSESPSGFSKHIQSNLDFSPVNSASSEENVKYSSSQPEPRTGLSLWDTSPSYIDKLVQGISFSQPTCPDHMLLNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETCEKLGYQWKKSCMNQVTISTTDRRNNKLIFKVNLLEMDDKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 55.0694 bits (131), Expect = 1.694e-32 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRVTEEAVAVKIVDMKRAVDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEPDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q8AYC9|CHK1_CHICK (Serine/threonine-protein kinase Chk1 OS=Gallus gallus OX=9031 GN=CHEK1 PE=1 SV=1) HSP 1 Score: 106.686 bits (265), Expect = 3.996e-32 Identity = 87/342 (25.44%), Postives = 145/342 (42.40%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLI-----GKIST-------------VKIQTNFQP------ENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H D+KPEN+++ K+ DFGLA H+ + ++ GT Y APE ++ D+++ V + GE W++ S Y DW ++ +++ + L L +PT R+TI IK WY + L G++S+ ++ T+F P E+ A SQP + L Q I+F++ P SS + +V+RMTRFF+ LDA ++ ++++ E+ + N T+ + F++ + + ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHSMGITHRDLKPENLLLDERDNLKISDFGLATVFKHNGRERLLNKMCGTLPYVAPELLRRPEFRAEPVDVWACGVVLTAMLAGELPWDQPSDSCQEYSDWKERKTYLAPWRKIDSAPLALLHKILTENPTARITIPDIKKDRWYCRPLKKGTKRGRVSSGGVTESPGALPKHIRSDTDFSPVKSALGEDKASYSTSQPEPGTGGALWDSSTGSIDRLVQGISFSQPACPEHMLLNSQLLGTPGSSQSPWQRLVRRMTRFFTKLDADGSYRSLRDVCEKMGYGWKQSCTNQVTISTTDRRNNKLIFKVNLLEMESRILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 54.6842 bits (130), Expect = 3.996e-32 Identity = 33/112 (29.46%), Postives = 58/112 (51.79%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ + + ++Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRRTEEAVAVKIVDMKRAADCPENIKKEICINKMLNHENVVKFYGHRREGATQYLFLEYCSGGELFDRIEPDIGMPEPEAQRFFQQLIAGVV 119
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q6DE87|CHK1_XENLA (Serine/threonine-protein kinase Chk1 OS=Xenopus laevis OX=8355 GN=chek1 PE=1 SV=2) HSP 1 Score: 103.219 bits (256), Expect = 3.304e-31 Identity = 87/352 (24.72%), Postives = 153/352 (43.47%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWY----RQGLI-----------GKISTVKIQTNF------QPENVAVIPKSQP---TMLKDHLNTSTEVNYDFPLSQPINFAELLMP--------------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFV 1494
VEY H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE + D++S + + GE W++ + YCDW +K+ +++ + L L +P R+TI IK W+ ++GL + +I+++ E + +QP T L + S+ ++ + + I+F++ P SS N +VKRMTRFF+ ++A+ ++ + + E+ + N T+ + F++ + + D IL+DFR +GDGL F F K+ K+++ V
Sbjct: 117 GVEYLHSIGITHRDIKPENLLLDERDQLKISDFGLATVFRHNGKERLLNKMCGTLPYVAPELIKSRAFHADPVDVWSCGIVLTAMLAGELPWDQPNEVCQEYCDWKEKNHYLTPWKKISATPLALLCKMLTENPQSRITIPDIKKDRWFTEIIKKGLKRSRVISGGSSDSSVLCKQIRSDIDISHFSHSEEKTALSSTQPEPRTALATWDSNSSYID-NLVQGKGISFSQPACPDNMLLNSQLIGTPGSSQNVWQRLVKRMTRFFTKVNAESSYSNLMDTCEKMGYVLKKSCANEVTLSTTDRRNNKLIFKVNLVEMEDRILLDFRLSKGDGLEFKRHF---LKIKKKMDAV 464 HSP 2 Score: 55.0694 bits (131), Expect = 3.304e-31 Identity = 34/107 (31.78%), Postives = 54/107 (50.47%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEGA G V A N++ + +AVKIV + I++E I + L H NIV YG + +F+E G+LFD I G+ D K ++Q+
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRKTEEAVAVKIVDMTRAADCPENIKKEICINRMLSHTNIVRFYGHRREGNIQYLFLEYCRGGELFDRIEPDVGMPEQDAQKFFQQL 114
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q9N3Z3|CHK1_CAEEL (Serine/threonine-protein kinase chk-1 OS=Caenorhabditis elegans OX=6239 GN=chk-1 PE=1 SV=2) HSP 1 Score: 109.383 bits (272), Expect = 5.643e-31 Identity = 88/336 (26.19%), Postives = 155/336 (46.13%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV---DHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW------HDKHWASLTIQRLPVDMINFLIDALAHDPT-GRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQP--------ENVAVIPKSQPTMLKDHLNTSTEVN-----YDFPLSQPINFAELLM-------PSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVD-----SILVDFRRVRGDGLLFHTVFSEV 1464
+++ H +++VH DIKPEN++++G+ V K+ DFG+A + + GT Y+APE Y G D++S + + +TGE W++AS Y W ++ W + ++ L ++ + D T R TI++I+ WY+ G++ T N +P EN+ +++ + + HL T E + + SQP +LL+ +++N +V RMTRF D + T+ +V E H + + + VT +V+ + +Y D ++VDFRR RGDG+ F +F +V
Sbjct: 137 GLKFIHDNDVVHRDIKPENLLLTGTHVLKISDFGMATLYRNKGEERLLDLSCGTIPYAAPELCAGKKYRGPPVDVWSSGIVLIAMLTGELPWDRASDASQSYMGWISNTSLDERPWKKIDVRAL------CMLRKIVTDKTDKRATIEQIQADPWYQHNF-GQVET----PNGRPLKRARNNDENITCTQQAECSAKRRHLETPNEKSTLAERQNASFSQPTKTEDLLLTQHIDMSQTNSNLLQRMVCRMTRFCVVTDIRSTYQKVARASE-HAGFGVRETDDYRLLVTWR----EVSMMVSLYTMGDIPDKPRVMVDFRRSRGDGIQFKKMFMDV 456 HSP 2 Score: 48.1358 bits (113), Expect = 5.643e-31 Identity = 33/111 (29.73%), Postives = 56/111 (50.45%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAV--KIVRSMINEKLRKRIREEFRIQKNLF---HRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
Y + +GEGA G V N +N + A KI + ++ IR+E+ +QK + H N++ + G+ N F +F+E G+LFD I CG++ + +KQ+
Sbjct: 24 YRVVQTLGEGAFGEVLLIVNTKNPEVAAAMKKINIANKSKDFIDNIRKEYLLQKRVSAVGHDNVIRMIGMRNDPQFYYLFLEYADGGELFDKIEPDCGMSPVFAQFYFKQL 134
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Match: sp|Q91ZN7|CHK1_RAT (Serine/threonine-protein kinase Chk1 OS=Rattus norvegicus OX=10116 GN=Chek1 PE=2 SV=1) HSP 1 Score: 99.7525 bits (247), Expect = 6.543e-30 Identity = 97/354 (27.40%), Postives = 149/354 (42.09%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLK-DHLNT-STEVNYDFPLSQP----------------------INFAELLMP--------------SSNNETSSIVKRMTRFFSTLDA-------KETFDRV-----KNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
V Y H I H DIKPEN+++ K+ DFGLA H+ + ++ GT Y APE ++ D++S + + GE W++ S Y DW +K +++ + L L +P+ R+TI IK WY + L + + E+ + K + L +N+ S+E N F SQP I+F++ P SS N +VKRMTRFF+ LDA KETF+++ K+ + Q T++R N I F+I + + + ILVDFR +GDGL F F ++
Sbjct: 117 GVVYLHGIGITHRDIKPENLLLDERDNLKISDFGLATVFRHNNRERLLNKMCGTLPYVAPELLKRKEFHAEPVDVWSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLALLHKILVENPSARITIPDIKKDRWYNKPLNRGAKRPRATSGGMSESSSGFSKHIHSNLDFSPINSGSSEENVKFSSSQPEPRTGLSLWDTGPSNVDKLVQGISFSQPTCPDHMLVNSQLLGTPGSSQNPWQRLVKRMTRFFTKLDADKSYQCLKETFEKLGYQWKKSCMNQVTVSTMDRRNNKLI------------FKINLVEMDEKILVDFRLSKGDGLEFKRHFLKI 458 HSP 2 Score: 53.9138 bits (128), Expect = 6.543e-30 Identity = 34/112 (30.36%), Postives = 57/112 (50.89%), Query Frame = 2
Query: 242 SYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
+++ +GEGA G V A N+ + +AVKIV + I++E I K L H N+V+ YG + +F+E + G+LFD I G+ D + + Q+ + +V
Sbjct: 8 DWDLVQTLGEGAYGEVQLAVNRITEEAVAVKIVDMKRAIDCPENIKKEICINKMLNHENVVKFYGHRREGNIQYLFLEYCSGGELFDRIEPDIGMPEQDAQRFFHQLMAGVV 119
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A4S2M8J7 (Protein kinase domain-containing protein OS=Opisthorchis felineus OX=147828 GN=CRM22_002870 PE=4 SV=1) HSP 1 Score: 229.18 bits (583), Expect = 9.269e-76 Identity = 136/360 (37.78%), Postives = 194/360 (53.89%), Query Frame = 1
Query: 559 NAVEYCHKHN-IVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFE-DCHYCDWHDKH--WASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKI----------------------STVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNET-------SSIVKRMTRFFSTLDAKE-TFDRVKNIIEQHHHC--TINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILP 1530
+A+ Y H I H+D+KPEN+M++ KLIDFG AVD S P +KG +GTT+Y+APE F C Y+G D+FSL VT LTAVTG+R W++A+ + D Y W D+ A RLP D+ NFL LAH P+ RLT+D ++ H W+R+GL+ ++ S+V + + QP+ A +P SQP + L + D LSQP + +LM S + S ++KRMTRFFS L + E T + ++ +I Q T N + + + FRI +Y SIL D RR+ GDGLLFH ++ + + VNE+HPYL++ILP
Sbjct: 174 DALHYLHTDQLIAHLDVKPENIMLTRKDRAKLIDFGWAVDLSKNPVLKGPVGTTSYAAPEVFGRCPYSGPPVDIFSLGVTLLTAVTGQRCWSRANPDVDPIYKQWVDRRDLHAGPIFSRLPPDLANFLTRVLAHQPSERLTVDGVRSHPWFRRGLLRRLTPVAPPPRPVSTNRETYQDSLESSVSCRASLQPD--APVPSSQP--VYTCLTQADPTANDLRLSQPADMTSMLMASQSANPDDMRLGFSCLMKRMTRFFSALSSVEHTMEVIRAVIIQRRQLRPTSKGPGNLMVQIRNQ-DHPGTAFRILLYPLAGSILADCRRISGDGLLFHQTYAAFYADLAAMGAVNESHPYLRMILP 528 HSP 2 Score: 85.5001 bits (210), Expect = 9.269e-76 Identity = 48/133 (36.09%), Postives = 74/133 (55.64%), Query Frame = 2
Query: 209 LKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVR------SMINE-----KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
++ E +P LP Y++S+ + EG +G V+ N E LA K+V S+ N K+R +R+E +Q+ L H ++ VYGI NT SF +FME L G+LF+ IPIG G++ F Y Q+ ++
Sbjct: 44 IQREGKPILPPGYQLSHCLAEGGMGRVFLVVNPETKDCLAAKVVSVYGRMLSVKNAEENVAKIRADLRQEAELQRRLKHHHVATVYGIRNTPSFTFMFMELLPGGELFERIPIGVGLHVQQMFIYYSQILDAL 176
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: H2KQ04 (Serine/threonine-protein kinase chk1 OS=Clonorchis sinensis OX=79923 GN=CLF_102933 PE=4 SV=1) HSP 1 Score: 225.713 bits (574), Expect = 1.099e-74 Identity = 135/360 (37.50%), Postives = 193/360 (53.61%), Query Frame = 1
Query: 559 NAVEYCHKHN-IVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFE-DCHYCDWHDKH--WASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKIS----------------------TVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNET-------SSIVKRMTRFFSTLDAKE-TFDRVKNIIEQHHHC--TINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILP 1530
+A+ Y H I H+D+KPEN+M++ KLIDFG AVD P +KG +GTT+Y+APE F C Y+G D+FSL VT LTAVTG+R W++A+ + D Y W D+ A RLP D+ NFL LAH P+ RLT+D I+ H W+R+GL+ +++ +V + + QP+ A +P SQP + L D LSQP + +LM S + S ++KRMTRFFS L + E T + ++ +I Q T N + + + FRI +Y SIL D RR+ GDGLLFH ++ ++ + VNE+HPYL++ILP
Sbjct: 221 DALHYLHTDQLIAHLDVKPENIMLTRKDRAKLIDFGWAVDLRKNPALKGPVGTTSYAAPEVFGRCSYSGPPVDIFSLGVTLLTAVTGQRCWSRANPDVDPIYKQWVDRRDLHAGPIFSRLPPDLANFLTRVLAHRPSERLTVDGIRSHPWFRRGLLRRLTPVAPPPRPVSTNRETHQDSLEFSVSSRASLQPD--APVPSSQP--VYTCLTQVDPTANDLRLSQPADMTSMLMASQSANPDDMRLGFSCLMKRMTRFFSALSSVEHTMEVIRAVILQRRQLRPTSKGPGNLMVQIRNQ-DHPGTAFRILLYPLAGSILADCRRISGDGLLFHQTYAALYADLVAMGAVNESHPYLRMILP 575 HSP 2 Score: 85.5001 bits (210), Expect = 1.099e-74 Identity = 48/133 (36.09%), Postives = 74/133 (55.64%), Query Frame = 2
Query: 209 LKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVR------SMINE-----KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
++ E +P LP Y++S+ + EG +G V+ N E LA K+V S+ N K+R +R+E +Q+ L H ++ VYGI NT SF +FME L G+LF+ IPIG G++ F Y Q+ ++
Sbjct: 91 IQREGKPILPPGYQLSHCLAEGGMGRVFLVVNPETKDCLAAKVVSVYGRMLSVKNAEENVAKIRADLRQEAELQRRLKHHHVATVYGIRNTPSFTFMFMELLPGGELFERIPIGVGLHVQQMFIYYSQILDAL 223
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A3R7H2M8 (Serine/threonine-protein kinase Chk1 OS=Clonorchis sinensis OX=79923 GN=Chek1 PE=4 SV=1) HSP 1 Score: 224.942 bits (572), Expect = 2.115e-74 Identity = 135/360 (37.50%), Postives = 193/360 (53.61%), Query Frame = 1
Query: 559 NAVEYCHKHN-IVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFE-DCHYCDWHDKH--WASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKIS----------------------TVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNET-------SSIVKRMTRFFSTLDAKE-TFDRVKNIIEQHHHC--TINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILP 1530
+A+ Y H I H+D+KPEN+M++ KLIDFG AVD P +KG +GTT+Y+APE F C Y+G D+FSL VT LTAVTG+R W++A+ + D Y W D+ A RLP D+ NFL LAH P+ RLT+D I+ H W+R+GL+ +++ +V + + QP+ A +P SQP + L D LSQP + +LM S + S ++KRMTRFFS L + E T + ++ +I Q T N + + + FRI +Y SIL D RR+ GDGLLFH ++ ++ + VNE+HPYL++ILP
Sbjct: 300 DALHYLHTDQLIAHLDVKPENIMLTRKDRAKLIDFGWAVDLRKNPALKGPVGTTSYAAPEVFGRCSYSGPPVDIFSLGVTLLTAVTGQRCWSRANPDVDPIYKQWVDRRDLHAGPIFSRLPPDLANFLTRVLAHRPSERLTVDGIRSHPWFRRGLLRRLTPVAPPPRPVSTNRETHQDSLEFSVSSRASLQPD--APVPSSQP--VYTCLTQVDPTANDLRLSQPADMTSMLMASQSANPDDMRLGFSCLMKRMTRFFSALSSVEHTMEVIRAVILQRRQLRPTSKGPGNLMVQIRNQ-DHPGTAFRILLYPLAGSILADCRRISGDGLLFHQTYAALYADLVAMGAVNESHPYLRMILP 654 HSP 2 Score: 85.5001 bits (210), Expect = 2.115e-74 Identity = 48/133 (36.09%), Postives = 74/133 (55.64%), Query Frame = 2
Query: 209 LKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVR------SMINE-----KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
++ E +P LP Y++S+ + EG +G V+ N E LA K+V S+ N K+R +R+E +Q+ L H ++ VYGI NT SF +FME L G+LF+ IPIG G++ F Y Q+ ++
Sbjct: 170 IQREGKPILPPGYQLSHCLAEGGMGRVFLVVNPETKDCLAAKVVSVYGRMLSVKNAEENVAKIRADLRQEAELQRRLKHHHVATVYGIRNTPSFTFMFMELLPGGELFERIPIGVGLHVQQMFIYYSQILDAL 302
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: A0A183QUM2 (Protein kinase domain-containing protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1) HSP 1 Score: 220.32 bits (560), Expect = 2.481e-74 Identity = 139/363 (38.29%), Postives = 190/363 (52.34%), Query Frame = 1
Query: 559 NAVEYCHK-HNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASF-EDCHYCDW--HDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAV----------------------IPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNET----------SSIVKRMTRFFSTLDAKE-TFDRVKNIIEQHHH--CTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILP 1530
+A+ Y H I H+D+KPEN+M++ KLIDFG AVD I+G +GTT+Y+APE F Y G ADLFSL VT LTAVTG+R W++AS+ D Y W H RLP D+ NFL ALAH P+ RLTI+ IK+H WYR GL +++ V + Q V+V +P SQPT + H + +N D LSQP + +LM S S +++RMTRFFS L + E T + ++ ++ Q H TI N + + + FR+ +Y SILVD RRV GDGLLFH ++ ++ + VN HPYL ++LP
Sbjct: 176 DALNYLHNDQQIAHLDVKPENIMLTKKDRAKLIDFGWAVDLRKQKLIQGAVGTTSYAAPEVFGKNPYFGPPADLFSLGVTLLTAVTGQRCWSRASYTSDPVYRQWTEHKDLHTGPVFSRLPPDLANFLTRALAHRPSDRLTIESIKNHPWYRLGLARRLTPVASPRSRQDSGVSVNTVVPNSDRTNTSRSAYQGDAPVPSSQPTPVC-HAQLDSIIN-DLHLSQPADTDSMLMASQMVGDIDYVKDRLGFSCLMRRMTRFFSALSSVEHTMEVIRAVVVQRRHLRATIKGSNNLLVDIKTP-EHPGTAFRVLLYPLAGSILVDCRRVSGDGLLFHQTYAAIYTDLSAMGAVNITHPYLHILLP 535 HSP 2 Score: 90.1225 bits (222), Expect = 2.481e-74 Identity = 49/139 (35.25%), Postives = 78/139 (56.12%), Query Frame = 2
Query: 197 LMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV------------RSMIN-EKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
+ + +K+E +P LP Y+IS+ + EG IG VY N + LA K+V ++ +N ++R +R+E +Q+ L H NI +YGI NT +F +FME L G+LF+ IPIG G++ F Y Q+ ++
Sbjct: 40 IQKSIKTEAQPILPPGYQISHCLAEGGIGRVYLIVNPDTKDCLAAKVVNVYGQSKLRSNTKAEVNVSQVRAELRQEAELQRRLKHHNIATLYGIRNTPTFTFMFMELLPGGELFERIPIGIGLHVQQMFIYYAQILDAL 178
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Match: G4VTW8 (Serine/threonine kinase OS=Schistosoma mansoni OX=6183 GN=Smp_077180 PE=4 SV=1) HSP 1 Score: 219.164 bits (557), Expect = 5.504e-74 Identity = 139/363 (38.29%), Postives = 189/363 (52.07%), Query Frame = 1
Query: 559 NAVEYCHK-HNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASF-EDCHYCDW--HDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENV----------------------AVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNET----------SSIVKRMTRFFSTLDAKE-TFDRVKNIIEQHHH--CTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILP 1530
+A+ Y H I H+D+KPEN+M++ KLIDFG AVD I+G +GTT+Y+APE F Y G ADLFSL VT LTAVTG+R W++AS+ D Y W H RLP D+ NFL ALAH P+ RLTI+ IK+H WYR GL +++ V + Q V A +P SQPT + H + +N D LSQP + +LM S S +++RMTRFFS L + E T + ++ ++ Q H TI N + + + FR+ +Y SILVD RRV GDGLLFH ++ ++ + VN HPYL ++LP
Sbjct: 176 DALNYLHNDQQIAHLDVKPENIMLTKKDRAKLIDFGWAVDLRKQKLIQGAVGTTSYAAPEVFGKNPYFGPPADLFSLGVTLLTAVTGQRCWSRASYTSDPVYRQWTEHKDLHTGPVFSRLPPDLANFLTRALAHRPSDRLTIESIKNHPWYRLGLARRLTPVASPRSRQDSGVSANTVVPNSDRTNTSRSTYQGDAPVPSSQPTPVC-HAQLDSIIN-DLHLSQPADTDSMLMASQMVGDIDYVKDRLGFSCLMRRMTRFFSALSSVEHTMEVIRAVVVQRRHLRATIRGSNNLLVDIKTP-EHPGTAFRVLLYPLAGSILVDCRRVSGDGLLFHQTYAAIYTDLSAMGAVNITHPYLHILLP 535 HSP 2 Score: 90.1225 bits (222), Expect = 5.504e-74 Identity = 50/135 (37.04%), Postives = 72/135 (53.33%), Query Frame = 2
Query: 197 LMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRK-------------RIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+ + +K+E +P LP Y+IS+ + EG IG VY N + LA K+V KLR +R+E +Q+ L H NI +YGI NT +F +FME L G+LF+ IPIG G++ F Y Q+
Sbjct: 40 IQKSIKTEAQPILPPGYQISHCLAEGGIGRVYLIVNPDTKDCLAAKVVNVYGQSKLRSNTKAEVNVSQVRAELRQEAELQRRLKHHNIATLYGIRNTPTFTFMFMELLPGGELFERIPIGIGLHVQQMFIYYAQI 174
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: chek1 (checkpoint kinase 1 [Source:NCBI gene;Acc:103021956]) HSP 1 Score: 115.161 bits (287), Expect = 9.720e-35 Identity = 92/329 (27.96%), Postives = 144/329 (43.77%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCH-YCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQ-TNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPS--------SNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENH 1506
VEY H I H DIKPEN+++ K+ DFGLA H + RL GT Y APE ++ AD+++ + + GE W++ S E C Y DW K +++ + L L H P RLTI +IK H W+ + ++Q ++ QPE + + + D S SQP +L+ S S N +VKRMTRFF+TL+A+++ +++ + N T + F++ + + IL+DFR +GDGL F +F K+ ++L + N
Sbjct: 116 GGVEYLHSIGITHRDIKPENILLDDKDNLKISDFGLATMFRHRGRERMLTRLCGTLPYVAPELMSRPEFHAQPADIWACGIVLTAMLAGELPWDQPS-ESCQEYFDWLQKKTYLTPWKKIDSIPLGLLTKMLLHSPEDRLTIPQIKKHRWFSRSFKSGAVEDRVQISSSQPEPQGLWEVREDVVHSDGAQVS--------FSQPACPDHMLLGSQLMGTPGASQNPWQRLVKRMTRFFTTLNAEKSCTALRDACVAMGYTWKQSCTNQATVSTLDRRNNKLIFKVHFLEMEERILLDFRLSKGDGLEFKRIF---LKLKQKLADIISNQ 432 HSP 2 Score: 51.9878 bits (123), Expect = 9.720e-35 Identity = 29/108 (26.85%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 239 NSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEGA G V N++ + +AVK+V + + +++E + K L H NIV +G + IF+E + G+LFD I G+ + + ++Q+
Sbjct: 7 QDWDLVQTLGEGAYGEVRLLVNRKTEEAVAVKVVDTSRAKDCLDNVKKEVCVHKMLSHANIVRFFGHRSEGPIQYIFLEYCSGGELFDRIEPDVGMPEKEAHRFFQQL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: chek1 (checkpoint kinase 1 [Source:NCBI gene;Acc:103021956]) HSP 1 Score: 108.227 bits (269), Expect = 1.021e-32 Identity = 98/348 (28.16%), Postives = 150/348 (43.10%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGRL-GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCH-YCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWY---------RQ----GLIGKI--STVKIQTNFQPENVAVIPKSQPT-----MLKDHLNTSTEVNYDFPLSQPINFAELLMPS--------SNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENH 1506
VEY H I H DIKPEN+++ K+ DFGLA H + RL GT Y APE ++ AD+++ + + GE W++ S E C Y DW K +++ + L L H P RLTI +IK H W+ RQ G + K+ S + E+ I SQP +++ + S F SQP +L+ S S N +VKRMTRFF+TL+A+++ +++ + N T + F++ + + IL+DFR +GDGL F +F K+ ++L + N
Sbjct: 116 GGVEYLHSIGITHRDIKPENILLDDKDNLKISDFGLATMFRHRGRERMLTRLCGTLPYVAPELMSRPEFHAQPADIWACGIVLTAMLAGELPWDQPS-ESCQEYFDWLQKKTYLTPWKKIDSIPLGLLTKMLLHSPEDRLTIPQIKKHRWFSRSFKSAIKRQSDTHGPVTKLLRSDSEQLARTNSEDRVQISSSQPEPQGLWEVREDVVHSDGAQVSF--SQPACPDHMLLGSQLMGTPGASQNPWQRLVKRMTRFFTTLNAEKSCTALRDACVAMGYTWKQSCTNQATVSTLDRRNNKLIFKVHFLEMEERILLDFRLSKGDGLEFKRIF---LKLKQKLADIISNQ 457 HSP 2 Score: 51.9878 bits (123), Expect = 1.021e-32 Identity = 29/108 (26.85%), Postives = 55/108 (50.93%), Query Frame = 2
Query: 239 NSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQM 562
+++ +GEGA G V N++ + +AVK+V + + +++E + K L H NIV +G + IF+E + G+LFD I G+ + + ++Q+
Sbjct: 7 QDWDLVQTLGEGAYGEVRLLVNRKTEEAVAVKVVDTSRAKDCLDNVKKEVCVHKMLSHANIVRFFGHRSEGPIQYIFLEYCSGGELFDRIEPDVGMPEKEAHRFFQQL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: sik1 (serine/threonine-protein kinase SIK2-like [Source:NCBI gene;Acc:103047200]) HSP 1 Score: 74.3294 bits (181), Expect = 6.033e-21 Identity = 46/149 (30.87%), Postives = 70/149 (46.98%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPV----DMINFLIDALAHDPTGRLTIDRIKDHIW 996
AV+YCH+H+IVH D+K EN+++ + KL DFG + + G+ Y+APE F +Y G + D++SL V V G ++ S + R+P D N + L DP R+T+ +IK H W
Sbjct: 135 AVDYCHRHHIVHRDLKTENLLLDANMNIKLADFGFGNFYKTGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGESLPALRQRVTEGRF-------RIPFFMSEDCENLIRKMLVVDPAKRITVAQIKQHRW 276 HSP 2 Score: 46.595 bits (109), Expect = 6.033e-21 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
YEI +G+G VV A++K +A+KI+ ++ +N ++I E +I K L H +I+++Y ++ T + I E G++FD +
Sbjct: 26 YEILRTLGKGNFAVVKLARHKVTKTQVAIKIIDKTRLNSSNLEKIYREVQIMKLLNHPHIIKLYQVMETKDMLYIVTEYAKNGEMFDFL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: sik1 (serine/threonine-protein kinase SIK2-like [Source:NCBI gene;Acc:103047200]) HSP 1 Score: 74.3294 bits (181), Expect = 6.544e-21 Identity = 46/149 (30.87%), Postives = 70/149 (46.98%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPV----DMINFLIDALAHDPTGRLTIDRIKDHIW 996
AV+YCH+H+IVH D+K EN+++ + KL DFG + + G+ Y+APE F +Y G + D++SL V V G ++ S + R+P D N + L DP R+T+ +IK H W
Sbjct: 135 AVDYCHRHHIVHRDLKTENLLLDANMNIKLADFGFGNFYKTGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDIWSLGVVLYVLVCGSLPFDGESLPALRQRVTEGRF-------RIPFFMSEDCENLIRKMLVVDPAKRITVAQIKQHRW 276 HSP 2 Score: 46.2098 bits (108), Expect = 6.544e-21 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
YEI +G+G VV A++K +A+KI+ ++ +N ++I E +I K L H +I+++Y ++ T + I E G++FD +
Sbjct: 26 YEILRTLGKGNFAVVKLARHKVTKTQVAIKIIDKTRLNSSNLEKIYREVQIMKLLNHPHIIKLYQVMETKDMLYIVTEYAKNGEMFDFL 114
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Match: snrka (SNF related kinase [Source:NCBI gene;Acc:103032037]) HSP 1 Score: 74.3294 bits (181), Expect = 9.319e-21 Identity = 47/151 (31.13%), Postives = 74/151 (49.01%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGL 1011
A+ YCH+ ++VH D+KPENV+ G+ KL DFG + + G+ AYSAPE L +Y D++SL V V G+ + +A+ + K+ + D+IN + L DP R +++ I+ H W QG+
Sbjct: 126 AISYCHRLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNKFQPGKKLTTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVCGQPPFQEANDSETLTMIMDCKYTVPTHVSNACKDLINRM---LQRDPKRRASLEEIESHPWL-QGV 272 HSP 2 Score: 45.8246 bits (107), Expect = 9.319e-21 Identity = 29/104 (27.88%), Postives = 56/104 (53.85%), Query Frame = 2
Query: 200 MQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
M +K + K+ Y++ +G G VV A++ G+ +AVK++ ++ ++ + +E R K + H NIV +Y +I+T + + + +E GD+FD I
Sbjct: 1 MAGIKRGYDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDTVATGHLFQEVRCMKLVQHPNIVRLYEVIDTQTKLYLILELGDGGDMFDYI 104
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006410.1 (pep scaffold:Pmarinus_7.0:GL476370:17405:31068:-1 gene:ENSPMAG00000005688.1 transcript:ENSPMAT00000006410.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 68.1662 bits (165), Expect = 4.554e-20 Identity = 44/154 (28.57%), Postives = 76/154 (49.35%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
+AV+YCH+ NIVH D+K EN+++ K+ DFG + + +++ + G+ Y+APE F Y G + D++SL V T V+G ++ + ++ K+ R+P D N L L +P R ++++I W G
Sbjct: 147 SAVQYCHQKNIVHRDLKAENLLLDVDMHIKIADFGFSNEFTLVNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELREKVLRGKY-------RIPFYMSTDCENLLKRFLVLNPIKRGSLEQIMRDRWMNVG 293 HSP 2 Score: 46.9802 bits (110), Expect = 4.554e-20 Identity = 28/89 (31.46%), Postives = 51/89 (57.30%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y + IG+G V A++ G+ +A+KI+ ++ +N +++ E RI K L H NIV++Y +I T + + ME + G++FD +
Sbjct: 39 YRLLKTIGKGNFAKVKLARHILTGREVAIKIIDKTQLNPTSLQKLFREVRIMKCLNHPNIVKLYEVIETEKTLYLVMEYASGGEVFDYL 127
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: snrka (SNF related kinase a [Source:ZFIN;Acc:ZDB-GENE-040718-84]) HSP 1 Score: 68.1662 bits (165), Expect = 7.462e-20 Identity = 45/147 (30.61%), Postives = 70/147 (47.62%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIW 996
+A+ YCH+ ++VH D+KPENV+ G+ KL DFG + + G+ AYSAPE L +Y D++SL V V G + +A+ + K+ I R D+I + L DP R ++ ++ H W
Sbjct: 126 HAISYCHRLHVVHRDLKPENVVFFEKQGLVKLTDFGFSNLFQPGTKLATSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILYMLVCGHPPFQEANDSETLTMIMDCKYTVPAHISRECKDLIARM---LQRDPRRRASLQEMEGHPW 269 HSP 2 Score: 46.595 bits (109), Expect = 7.462e-20 Identity = 26/90 (28.89%), Postives = 51/90 (56.67%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRI-REEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y++ +G G VV A++ G+ +AVK++ +S ++ + ++E R K + H N+V +Y +I+T + + + +E GDL+D I
Sbjct: 16 YDLEKTLGRGHFAVVKLARHVFTGEKVAVKVIDKSKLDPMATSHLFQQEVRCMKLVQHPNVVRLYEVIDTQTKLYLILELADGGDLYDYI 105
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 66.6254 bits (161), Expect = 1.082e-19 Identity = 43/154 (27.92%), Postives = 75/154 (48.70%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
+AV+YCH+ +IVH D+K EN+++ K+ DFG + + ++ + G+ Y+APE F Y G + D++SL V T V+G ++ + ++ K+ R+P D N L L +P R ++++I W G
Sbjct: 162 SAVQYCHQKHIVHRDLKAENLLLDADMNIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY-------RIPFYMSTDCENLLKRFLVLNPAKRGSLEQIMKDRWMNVG 308 HSP 2 Score: 47.7506 bits (112), Expect = 1.082e-19 Identity = 30/99 (30.30%), Postives = 56/99 (56.57%), Query Frame = 2
Query: 215 SEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
+E+E +Y + IG+G V A++ G+ +AVKI+ ++ +N +++ E RI K L H NIV+++ +I T + + ME + G++FD +
Sbjct: 44 AEVEHAHIGTYRLLKTIGKGNFAKVKLARHTLTGREVAVKIIDKTQLNPSSLQKLFREVRIMKILNHPNIVKLFEVIETEKTLYLVMEYASGGEVFDYL 142
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: chek1 (checkpoint kinase 1 [Source:ZFIN;Acc:ZDB-GENE-040426-896]) HSP 1 Score: 87.0409 bits (214), Expect = 5.718e-19 Identity = 89/337 (26.41%), Postives = 139/337 (41.25%), Query Frame = 1
Query: 571 YCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGR-LGTTAYSAPETFL-DCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWY-----RQGLIGKISTVKIQT-------NFQPENVAVIPK------SQPTMLKDH----LNTSTEVNY---DFPLSQPINFAELLMPS--------SNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVD---SILVDFR--RVRGDGLLFHTVF 1455
Y H I H DIKPEN+++ + K+ DFGLA HS R GT Y APE D + D+++ V + GE W++ + Y DW + +++ + L L D R TI I+ + W+ R G+ ++ + + + P ++A + SQP H +T + +N SQP++ +L+ S S N +VKRMTRFF A+E R+ + E H ++ Q VT + N IF F++ +L+DFR + +GDGL F F
Sbjct: 1 YLHSLGITHRDIKPENLLLDENDTLKISDFGLATVFRHSGRERRLSRTCGTLPYVAPELLQPDPTFRAAPVDVWACGVVLTAMLAGELPWDQPAMSCMEYVDWRQRRLHVTPWRKIDSLPLALLRKVLEPDAEKRATIPEIRQNSWFNHTLKRGGVRRELGESGLSSKRICSGIDLSPGSLASSDEQVRYSVSQPEPRLTHPSPSQSTGSSINALLGGLSFSQPLHPDHMLLSSQLQSTPVSSQNPWLRLVKRMTRFFVRGAAEEVRSRLSEVCEGH---GLSYRQTCPTQVTLSTLDRRNNKLIFKVTFLEMDGRLLLDFRLSKRQGDGLEFKRHF 334
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Match: MARK3 (microtubule affinity regulating kinase 3 [Source:HGNC Symbol;Acc:HGNC:6897]) HSP 1 Score: 65.0846 bits (157), Expect = 2.862e-18 Identity = 42/150 (28.00%), Postives = 73/150 (48.67%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIW 996
+AV+YCH+ +IVH D+K EN+++ K+ DFG + + ++ + G+ Y+APE F Y G + D++SL V T V+G ++ + ++ K+ R+P D N L L P R ++++I W
Sbjct: 176 SAVQYCHQKHIVHRDLKAENLLLDADMNIKIADFGFSNEFTLGSKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY-------RIPFYMSTDCENLLKRFLVLHPLKRGSLEQIMKDRW 318 HSP 2 Score: 44.2838 bits (103), Expect = 2.862e-18 Identity = 27/89 (30.34%), Postives = 50/89 (56.18%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y + IG+G V A++ G+ +AVKI+ ++ +N +++ E RI K L H NIV+++ +I + + ME + G++FD +
Sbjct: 68 YRLLKTIGKGNFAKVKLAKHTLTGQEVAVKIIDKTQLNTSSLQKLFREVRIMKVLNHPNIVKLFEVIEMEKTLYLVMEYSSGGEVFDYL 156
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: IPL1 (Aurora kinase of chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in meiosis I, and kinetochore release from SPB clusters at meiotic exit; helps maintain condensed chromosomes during anaphase; required for SPB cohesion and prevention of multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; required for inhibition of karyopherin Pse1p upon SAC arrest [Source:SGD;Acc:S000006130]) HSP 1 Score: 67.3958 bits (163), Expect = 3.002e-18 Identity = 47/150 (31.33%), Postives = 74/150 (49.33%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQ---RLPVDMINFLIDALAHDPTGRLTIDRIKDHIW 996
NA++Y HK NI+H DIKPEN++I + V KL DFG ++ + K GT Y +PE +Y D ++L V +TG + + +D Y K A+L I+ + D + ++ L +DP R+ + +K H W
Sbjct: 212 ANALDYMHKKNIIHRDIKPENILIGFNNVIKLTDFGWSIINPPENRRKTVCGTIDYLSPEMVESREY-DHTIDAWALGVLAFELLTGAPPF-EEEMKDTTY-----KRIAALDIKMPSNISQDAQDLILKLLKYDPKDRMRLGDVKMHPW 354 HSP 2 Score: 41.2022 bits (95), Expect = 3.002e-18 Identity = 22/85 (25.88%), Postives = 44/85 (51.76%), Query Frame = 2
Query: 287 VYTAQNKENGKFLAVKIVR--SMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTL 535
VY +++ G A+K++ +I L+K+ R E IQ +L H N+ + YG + + + ME L G+++ ++ + N +
Sbjct: 118 VYCVRHRSTGYICALKVMEKEEIIKYNLQKQFRREVEIQTSLNHPNLTKSYGYFHDEKRVYLLMEYLVNGEMYKLLRLHGPFNDI 202
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: SSK22 (MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication [Source:SGD;Acc:S000000669]) HSP 1 Score: 55.8398 bits (133), Expect = 1.193e-17 Identity = 46/161 (28.57%), Postives = 69/161 (42.86%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLA----------VDHSVI-------PTIKGRLGTTAYSAPETFLDCDYTGFKA--DLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP------VDMINFLIDALAHDPTGRLT 969
+ Y H+ +VH DIKPEN+++ +G+ K +DFG A V ++ + ++ +GT Y APET G D+++L L TG R W+ D + + H A+ I +LP FL L DPT R T
Sbjct: 1145 GLAYLHQSGVVHRDIKPENILLDFNGIIKYVDFGTARTVVGSRTRTVRNAAVQDFGVETKSLNEMMGTPMYMAPETISGSAVKGKLGADDVWALGCVVLEMATGRRPWSNL---DNEWAIMY--HVAAGRIPQLPNRDEMTAAGRAFLERCLVQDPTMRAT 1300 HSP 2 Score: 50.447 bits (119), Expect = 1.193e-17 Identity = 33/94 (35.11%), Postives = 50/94 (53.19%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRK---RIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIG 517
++ + IG G G VY+A N ENG+ LAVK ++ ++K I+EE + + L H NIV+ YG+ + IFME G L ++ G
Sbjct: 1034 WQKRSFIGGGTFGQVYSAINLENGEILAVKEIKIHDTTTMKKIFPLIKEEMTVLEMLNHPNIVQYYGVEVHRDKVNIFMEYCEGGSLASLLDHG 1127
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: KCC4 (Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication [Source:SGD;Acc:S000000529]) HSP 1 Score: 60.4622 bits (145), Expect = 4.643e-16 Identity = 29/85 (34.12%), Postives = 45/85 (52.94%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTG 816
+ YCH IVH D+KPEN+++ K+ DFG+A + ++ G+ Y+APE Y GF +D++S V +TG
Sbjct: 139 GISYCHALGIVHRDLKPENLLLDSFYNIKIADFGMAALQTDADLLETSCGSPHYAAPEIVSGLPYEGFASDVWSCGVILFALLTG 223 HSP 2 Score: 40.817 bits (94), Expect = 4.643e-16 Identity = 25/98 (25.51%), Postives = 48/98 (48.98%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMI----------NEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
+++ +G G+ G V AQ++ G AVK++ I + L I E I K L H N++ +Y + T + + + +E +G+LF+++
Sbjct: 21 WKLGETLGFGSTGKVQLAQHERTGHRTAVKVISKSIFNNNGNHSNDDSVLPYNIEREIVIMKLLSHPNVLSLYDVWETNNNLYLILEYAEKGELFNLL 118
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: KIN2 (Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; KIN2 has a paralog, KIN1, that arose from the whole genome duplication [Source:SGD;Acc:S000004086]) HSP 1 Score: 77.0258 bits (188), Expect = 2.670e-15 Identity = 50/162 (30.86%), Postives = 79/162 (48.77%), Query Frame = 1
Query: 529 HVRRI*NL*TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLT--IQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
H R+ +A++Y H +NIVH D+K EN+MIS SG K+IDFGL+ + G+ ++APE YTG + D++S + V G K F+D + H+K L +++I+ L + DP R T+ + +H W +G
Sbjct: 224 HARKFARGIASALQYLHANNIVHRDLKIENIMISSSGEIKIIDFGLSNIFDYRKQLHTFCGSLYFAAPELLKAQPYTGPEVDIWSFGIVLYVLVCG-----KVPFDDENSSILHEKIKKGKVDYPSHLSIEVISLLTRMIVVDPLRRATLKNVVEHPWMNRG 380
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Match: SAT4 (Ser/Thr protein kinase involved in salt tolerance; funtions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion [Source:SGD;Acc:S000000601]) HSP 1 Score: 58.9214 bits (141), Expect = 2.792e-15 Identity = 32/108 (29.63%), Postives = 59/108 (54.63%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFG------LAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDW 867
V Y H+ + H D+KPEN++++ GV K+ DFG +A + + I G G++ Y APE ++ ++ D+++ V ++ TG +LW+ A +D Y ++
Sbjct: 436 GVVYMHEMGVCHRDLKPENLLLTHDGVLKITDFGNSECFKMAWEKN-IHLSGGVCGSSPYIAPEEYIKEEFDPRPVDIWACGVIYMAMRTGRQLWSSAEKDDPFYMNY 542 HSP 2 Score: 39.6614 bits (91), Expect = 2.792e-15 Identity = 35/118 (29.66%), Postives = 53/118 (44.92%), Query Frame = 2
Query: 260 MIGEGAIGVVYTAQNK----ENG----KFLAVKIVR---SMINEKLRKRIREEFRIQKNLFHRNIVEVYGII-NTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSIV 577
++G+GA GVV Q K ++G K AVK + S EK KR+ EF I +L H NIV + + ME GDLF ++ + ++ +KQ+ +V
Sbjct: 321 VLGKGAFGVVRICQKKNVSSQDGNKSEKLYAVKEFKRRTSESAEKYSKRLTSEFCISSSLHHTNIVTTLDLFQDAKGEYCEVMEYCAGGDLFTLVVAAGKLEYMEADCFFKQLIRGVV 438
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO30712 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T001]) HSP 1 Score: 87.0409 bits (214), Expect = 5.002e-24 Identity = 87/354 (24.58%), Postives = 141/354 (39.83%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAV--DHSVIPTIKGR-LGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKIST---------------VKIQTNFQPEN----VAVIPKSQPTMLKDHLNTS-----------TEVNYDFPLSQPINFAELLMP--------SSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQ---HHHC---------TINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEV 1464
VEY H+ + H DIKPEN+++ K+ DFG A H + G+ GT Y APE DY AD++S + + + GE W++ S + Y W + ++ + L L +P+ R TI IK W+ + + VK+ N + A I SQP + HL++ E SQPI+ +L+ SS +VKRMTR S + + T ++K+ +++ + C TI+R +N + ++ + ++LVDFR +GDGL F F ++
Sbjct: 124 GVEYLHRQGVTHRDIKPENLLLDSDDNLKITDFGFATVFRHKGRERLLGKCCGTPPYVAPEVLGKMDYRAQPADIWSCGIVLVAMLAGELPWDEPSPRNREYLAWIENKINRTPWVKIDPQPLALLKKILLANPSKRCTIPLIKADRWFDRTFTVTTRSPGNSKSICNFIIYLYVKLCDNHCNNSWNGHFAHISASQPEP-RRHLDSGLGSAIGSTEDCGEAKEALWFSQPIHPDHMLLSSQLPCTPGSSQTPFQRLVKRMTRIHSKKNQETTLAKLKDALDKLSYSYKCTGVKLLTVETIDRRKNKIV------------MKVSLIDMAPAVLVDFRLSKGDGLEFKRQFLKI 464 HSP 2 Score: 42.3578 bits (98), Expect = 5.002e-24 Identity = 32/118 (27.12%), Postives = 56/118 (47.46%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV--------RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
++ +GEGA G V A N+E + +AVKI+ S ++ IR+E I + L ++++ Y S +F+E + G+LFD I G+ K +KQ+ + +
Sbjct: 8 WDFIETLGEGAYGEVKLAVNRETQEAIAVKIINIERVKAASSQNGTSIQDDIRKEICIHRMLQDVHVIKFYAQRVENSKQYLFLEYSSGGELFDRIEPDIGMPQAMAHKYFKQLLAGV 125
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO36587 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SI81]) HSP 1 Score: 70.4774 bits (171), Expect = 2.222e-21 Identity = 53/159 (33.33%), Postives = 77/159 (48.43%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIK--GRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKAS-------FEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQG 1008
A++YCHK ++VH D+KPENV+ + KL DFG + ++ IP K G+ AYSAPE L Y D++SL V V G+ +++A+ DC Y D D H + L N + L +P R ++ I H W +QG
Sbjct: 129 AIDYCHKLHVVHRDLKPENVIFFKNQDMAKLTDFGFS--NNFIPNEKLDTACGSLAYSAPEVLLGDAYEAPAVDVWSLGVILYMLVCGQAPFSEANDSETLTKIMDCRY-DVPD-HVSPLC--------KNLISRMLIREPHNRASLGEIMSHPWLQQG 275 HSP 2 Score: 50.0618 bits (118), Expect = 2.222e-21 Identity = 26/89 (29.21%), Postives = 53/89 (59.55%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y++ +G G VV A++ G+ +AVK++ ++ ++E R+ + +E R K + H N+V +Y +I+T + + + +E GD++D I
Sbjct: 19 YDLGETLGRGHFAVVKVARHVITGERVAVKVIDKTKLDEVSREHLLKEVRCMKLVQHPNVVRLYQVIDTNTKLYLILELGDGGDMYDYI 107
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO46035 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR22]) HSP 1 Score: 70.8626 bits (172), Expect = 1.303e-19 Identity = 45/151 (29.80%), Postives = 71/151 (47.02%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMI----NFLIDALAHDPTGRLTIDRIKDHIW 996
+A+EYCHK NIVH D+K EN+++ K+ DFG + +K G+ Y+APE F +Y G + D++SL V V G ++ ++ + + R+P M + + L DP R TI +I+ H W
Sbjct: 127 VSAIEYCHKMNIVHRDLKAENLLLDEDMNIKIADFGFSNIFQADKKLKTWCGSPPYAAPELFEGKEYLGPEVDIWSLGVVLYVLVCGALPFDGSTLQSLRSRVLDGRF-------RIPFFMSTECEHLIRHMLVRDPVKRFTIPQIRQHKW 270 HSP 2 Score: 43.8986 bits (102), Expect = 1.303e-19 Identity = 26/111 (23.42%), Postives = 58/111 (52.25%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
Y I IG+G VV A + +AVKI+ +S +++ +++ E ++ K L H +I++++ ++ T + + E ++G++FD + + + + Q+ S+I
Sbjct: 20 YNIEKTIGKGNFAVVKLATHCITKSKVAVKIIDKSQLDDDNLTKVKREVKVMKKLAHPHIIKLHEVMETERMLYLVTEYASKGEIFDYLVAHGRMQEKEAKNTFNQIVSAI 130
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO30768 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SZV3]) HSP 1 Score: 62.7734 bits (151), Expect = 6.199e-19 Identity = 39/150 (26.00%), Postives = 71/150 (47.33%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLP----VDMINFLIDALAHDPTGRLTIDRIKDHIW 996
+AV+YCH+ +++H D+K EN+++ K+ DFG + + + + G+ Y+APE F Y G + D++SL V T V+G ++ + ++ K+ R+P D N L L +P R +++ W
Sbjct: 146 SAVQYCHQKHVIHRDLKAENLLLDADMNIKIADFGFSNEFTPGNKLDTFCGSPPYAAPELFQGKKYDGPEVDVWSLGVILYTLVSGSLPFDGQNLKELRERVLRGKY-------RIPFYMSTDCENLLKKFLVLNPQKRARLEQTMTDKW 288 HSP 2 Score: 49.2914 bits (116), Expect = 6.199e-19 Identity = 29/89 (32.58%), Postives = 51/89 (57.30%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y + IG+G V A++ GK +A+KI+ ++ +N +++ E RI K L H NIV++Y +I T + + ME + G++FD +
Sbjct: 38 YRLIKTIGKGNFAKVKLAKHVPTGKEVAIKIIDKTQLNPSSLQKLFREVRIMKFLDHPNIVKLYEVIETDKTLYLVMEYASGGEVFDYL 126
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Match: EDO33625 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SRN4]) HSP 1 Score: 55.8398 bits (133), Expect = 3.595e-17 Identity = 34/92 (36.96%), Postives = 48/92 (52.17%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLA-VDHSVIPTIKGRL-----GTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTG 816
+AVEY H +VH D+K EN++++ + DFG A H + T K RL G+ AY+ PE Y G AD++SL V T V+
Sbjct: 170 DAVEYLHNKGVVHRDLKCENILLNRDNRILISDFGFARTQHVMADTGKRRLSQTFCGSYAYAPPEILRGIAYDGTLADIWSLGVVLYTMVSA 261 HSP 2 Score: 50.447 bits (119), Expect = 3.595e-17 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV--RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
Y + + +G+G+ VV A +++ K +AVKIV + ++ L K + E ++ K+L H N+V ++ I T S I I ++ GDL + I I + Y Q+ ++
Sbjct: 61 YALGDTLGKGSYAVVKAAYSRKLKKQVAVKIVTKKKAPDDYLTKFLPREIQVMKHLNHSNVVSLHEAIETSSRIYIILDLADNGDLLEYIRSNGAIPENEARLFYHQLVDAV 172
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: chek1 (checkpoint kinase 1 [Source:NCBI gene;Acc:101170780]) HSP 1 Score: 110.153 bits (274), Expect = 2.298e-35 Identity = 98/367 (26.70%), Postives = 156/367 (42.51%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGR-------LGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCH-YCDWHDKH-----WASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTN------FQPENVAVIPKSQPTMLKDHLNT-----STEVNYDFPLSQPINFAELLMPS--------SNNETSSIVKRMTRFFSTLDAKETFDRVKNIIE-----------QHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI 1533
AVEY HK I H DIKPEN+++ KL DFGLA + KGR GT Y APE +Y AD+++ + + GE W++ + E C Y DW + W IQ P+ +++ L L +P R+T + +K W+ QG + + + N ++ + SQP +E F SQP +L+ S S + +V+RMTRFF+T+DA T +K+ + Q T+++ N + F++ + +L+DFR +GDGL F +F ++ + K + VN K++LP+
Sbjct: 117 AVEYLHKLGITHRDIKPENILLDDKDNLKLTDFGLA----TMFRFKGRERRLNRLCGTLPYVAPELLSQSEYHAQPADIWACGIVLTAMLAGELPWDQPT-ESCQEYADWLQRKTYLPPWKK--IQPAPLSLLSKL---LQPNPNTRITGEELKKDRWFTQGCLSSGCSKVLHNNRDFMFRASSDDRMQLSSSQPDFAAGGWEAMLVFGQSEGQVSF--SQPTKPEHMLLGSQLLATPGASQSPWQKLVRRMTRFFTTVDADATLSALKDTCDALALGFKLTCRQVTLNTLDKRNNPLV------------FKVLLLHLNQKVLLDFRLSKGDGLEFKRLFVKIKQ--KLGDIVNSQ----KILLPV 453 HSP 2 Score: 58.9214 bits (141), Expect = 2.298e-35 Identity = 31/112 (27.68%), Postives = 61/112 (54.46%), Query Frame = 2
Query: 239 NSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
+++ +GEGA G V N++ + +AVK++ + E+ + +++E I K L H NIV +G + + +F+E T G+LFD I G+ D + ++Q+ +++
Sbjct: 7 QDWDVVQTLGEGAYGEVRLLVNRQTEEAVAVKVIDTSQAEECAENVKKEICIHKILNHCNIVRFFGHRREGTTVYLFLEYCTGGELFDRIEPDVGMPERDAHRFFQQLIAAV 118
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: ENSORLT00000006985.2 (SNF-related serine/threonine-protein kinase [Source:NCBI gene;Acc:101156261]) HSP 1 Score: 77.7962 bits (190), Expect = 2.762e-23 Identity = 48/149 (32.21%), Postives = 77/149 (51.68%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVM-ISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYR 1002
+A+ YCH+ ++VH D+KPENV+ + GV KL DFG + T+ G+ AYSAPE L +Y D++SL V V G+ + +A+ + K+ I + D+I + L DP R T+++I++H W +
Sbjct: 125 HAISYCHRLHVVHRDLKPENVVFLEKQGVVKLTDFGFSNRFQPGKTLNTSCGSLAYSAPEILLGDEYDAPAVDIWSLGVILFMLVCGQPPFQEANDSETLTMIMDCKYTVPPHISQACRDLIGHM---LQRDPKKRATLEQIENHEWLQ 270 HSP 2 Score: 50.8322 bits (120), Expect = 2.762e-23 Identity = 29/104 (27.88%), Postives = 57/104 (54.81%), Query Frame = 2
Query: 200 MQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
M LK + K+ Y++ +G G VV A++ G+ +AVK++ ++ ++ R + +E R K + H N+V +Y +I+T + + + +E GD++D I
Sbjct: 1 MAGLKRHHDGKIAGLYDLDKTLGRGHFAVVKLARHVFTGEKVAVKVIDKTKLDPVARGHLFQEVRCMKMVQHPNVVRLYEVIDTATKLYLILELGDGGDMYDCI 104
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: map3k4 (mitogen-activated protein kinase kinase kinase 4 [Source:NCBI gene;Acc:101174856]) HSP 1 Score: 72.7886 bits (177), Expect = 2.908e-21 Identity = 54/166 (32.53%), Postives = 83/166 (50.00%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVD-----HSVIPTIKGRLGTTAYSAPE--TFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKA--SFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKIS 1026
T A+ H+H IVH DIK N+ ++ SG+ KL DFG +V H++ + LGT AY APE T + G AD++SL + VTG+R W++ +F+ + K ++L + +FL L +P R T + DH + + L GK+S
Sbjct: 1261 TTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHK---PPIPEKLSTEGKDFLCHCLESEPKRRWTASMLLDHPFVKVRL-GKLS 1422 HSP 2 Score: 48.9062 bits (115), Expect = 2.908e-21 Identity = 31/97 (31.96%), Postives = 51/97 (52.58%), Query Frame = 2
Query: 230 KLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINE-KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIG 517
K+ ++ N IGEG G VYT N + G+ +A+K +R N+ K K +E +I + + H N+V +G+ + IFME G L ++ +G
Sbjct: 1151 KVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLG 1247
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: map3k4 (mitogen-activated protein kinase kinase kinase 4 [Source:NCBI gene;Acc:101174856]) HSP 1 Score: 71.2478 bits (173), Expect = 8.439e-21 Identity = 50/154 (32.47%), Postives = 76/154 (49.35%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVD-----HSVIPTIKGRLGTTAYSAPE--TFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKA--SFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDH 990
T A+ H+H IVH DIK N+ ++ SG+ KL DFG +V H++ + LGT AY APE T + G AD++SL + VTG+R W++ +F+ + K ++L + +FL L +P R T + DH
Sbjct: 1385 TTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHK---PPIPEKLSTEGKDFLCHCLESEPKRRWTASMLLDH 1535 HSP 2 Score: 48.9062 bits (115), Expect = 8.439e-21 Identity = 31/97 (31.96%), Postives = 51/97 (52.58%), Query Frame = 2
Query: 230 KLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINE-KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIG 517
K+ ++ N IGEG G VYT N + G+ +A+K +R N+ K K +E +I + + H N+V +G+ + IFME G L ++ +G
Sbjct: 1275 KVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLG 1371
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Match: map3k4 (mitogen-activated protein kinase kinase kinase 4 [Source:NCBI gene;Acc:101174856]) HSP 1 Score: 71.2478 bits (173), Expect = 8.658e-21 Identity = 50/154 (32.47%), Postives = 76/154 (49.35%), Query Frame = 1
Query: 556 TNAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVD-----HSVIPTIKGRLGTTAYSAPE--TFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKA--SFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDH 990
T A+ H+H IVH DIK N+ ++ SG+ KL DFG +V H++ + LGT AY APE T + G AD++SL + VTG+R W++ +F+ + K ++L + +FL L +P R T + DH
Sbjct: 1304 TTAINVLHEHGIVHRDIKGANIFLTSSGLIKLGDFGCSVKLRNNTHTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVLIEMVTGKRPWHEYEHNFQIMYKVGMGHK---PPIPEKLSTEGKDFLCHCLESEPKRRWTASMLLDH 1454 HSP 2 Score: 48.9062 bits (115), Expect = 8.658e-21 Identity = 31/97 (31.96%), Postives = 51/97 (52.58%), Query Frame = 2
Query: 230 KLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINE-KLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIG 517
K+ ++ N IGEG G VYT N + G+ +A+K +R N+ K K +E +I + + H N+V +G+ + IFME G L ++ +G
Sbjct: 1194 KVTFKWQRGNKIGEGQYGKVYTCINVDTGELMAMKEIRFQPNDHKTIKETADELKIFEGIKHPNLVRYFGVELHREEMYIFMEYCDEGTLEEVSKLG 1290
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000025533.1 (SMESG000025533.1) HSP 1 Score: 672.159 bits (1733), Expect = 0.000e+0 Identity = 321/325 (98.77%), Postives = 323/325 (99.38%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI 1533
+AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSL VTFLT+VTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINR QNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI
Sbjct: 138 DAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLTVTFLTSVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRCQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI 462 HSP 2 Score: 280.796 bits (717), Expect = 0.000e+0 Identity = 135/140 (96.43%), Postives = 138/140 (98.57%), Query Frame = 2
Query: 155 MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQSSI 574
MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQ+ ++
Sbjct: 1 MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQLLDAV 140
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000025533.1 (SMESG000025533.1) HSP 1 Score: 635.18 bits (1637), Expect = 0.000e+0 Identity = 303/314 (96.50%), Postives = 309/314 (98.41%), Query Frame = 1
Query: 592 VHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRYQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI 1533
++ ++ +NVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSL VTFLT+VTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINR QNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI
Sbjct: 132 IYKQMQSKNVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLTVTFLTSVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWYRQGLIGKISTVKIQTNFQPENVAVIPKSQPTMLKDHLNTSTEVNYDFPLSQPINFAELLMPSSNNETSSIVKRMTRFFSTLDAKETFDRVKNIIEQHHHCTINRCQNSFIHVTSAVSGFDVNFRIFIYKFVDSILVDFRRVRGDGLLFHTVFSEVFKMAKQLEFVNENHPYLKLILPI 445 HSP 2 Score: 285.419 bits (729), Expect = 0.000e+0 Identity = 138/138 (100.00%), Postives = 138/138 (100.00%), Query Frame = 2
Query: 155 MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQS 568
MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQS
Sbjct: 1 MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGCGINTLDGFKIYKQMQS 138
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000038583.1 (SMESG000038583.1) HSP 1 Score: 120.168 bits (300), Expect = 2.726e-32 Identity = 60/147 (40.82%), Postives = 85/147 (57.82%), Query Frame = 1
Query: 559 NAVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTGERLWNKASFEDCHYCDWHDKHWASLTIQRLPVDMINFLIDALAHDPTGRLTIDRIKDHIWY 999
N +EY H NI HMDIKP+N++I K+IDF ++D S IP+++G +G+ A+ APE L+ Y GF D+FSL +T T+ TG LW++A D Y +W + L VD+ FL L HDP R +I IK+ W+
Sbjct: 125 NVIEYLHSKNIAHMDIKPDNILIDMCKSVKIIDFENSLDFSKIPSVRGPIGSFAFGAPEINLNGFYRGFPCDIFSLHLTLATSATGRLLWSQAQDSDEKYKNWRSNDSRTAEFSDLNVDLFLFLKRVLIHDPRKRPSIRLIKEDHWF 271 HSP 2 Score: 38.5058 bits (88), Expect = 2.726e-32 Identity = 34/107 (31.78%), Postives = 52/107 (48.60%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIVRSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMIPIGC-GINTLDGFKIYKQM 562
Y +N+IG+G+ G V QN+ + K +A KI K E I K + H NI+++ G T + I+ME + G + + I C GI + F IY Q+
Sbjct: 25 YIFTNIIGKGSDGTVVKLQNQSSKKQIAAKI-------STNKNNSNEINILKKIGHENIIKMLGYEFTANQRIIYMEYV-EGVTLETLIIECNGIEMSELFPIYYQV 123
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000057003.1 (SMESG000057003.1) HSP 1 Score: 66.2402 bits (160), Expect = 1.585e-20 Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTG 816
A++YCH IVH D+K EN+++S K+ DFG A + V + G+ Y+APE F Y G + D++SL V T V+G
Sbjct: 142 AIQYCHHKQIVHRDLKAENLLLSNDLQIKIADFGFANNFDVKKQLDTFCGSPPYAAPELFQGRKYEGPEVDVWSLGVILYTLVSG 226 HSP 2 Score: 52.7582 bits (125), Expect = 1.585e-20 Identity = 28/89 (31.46%), Postives = 53/89 (59.55%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y++ IG+G V AQ+ GK +AVK++ ++ +N +++ E RI K + H NIV+++ +I + + + ME +RG++FD +
Sbjct: 33 YKLIKTIGKGNFAKVKLAQHVTTGKKVAVKVIDKTALNNSSLQKLFREVRIMKTIDHPNIVKLFEVIESERHVYLVMEYASRGEVFDYL 121
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Match: SMESG000057003.1 (SMESG000057003.1) HSP 1 Score: 66.2402 bits (160), Expect = 1.585e-20 Identity = 33/85 (38.82%), Postives = 48/85 (56.47%), Query Frame = 1
Query: 562 AVEYCHKHNIVHMDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDCDYTGFKADLFSLIVTFLTAVTG 816
A++YCH IVH D+K EN+++S K+ DFG A + V + G+ Y+APE F Y G + D++SL V T V+G
Sbjct: 130 AIQYCHHKQIVHRDLKAENLLLSNDLQIKIADFGFANNFDVKKQLDTFCGSPPYAAPELFQGRKYEGPEVDVWSLGVILYTLVSG 214 HSP 2 Score: 52.7582 bits (125), Expect = 1.585e-20 Identity = 28/89 (31.46%), Postives = 53/89 (59.55%), Query Frame = 2
Query: 245 YEISNMIGEGAIGVVYTAQNKENGKFLAVKIV-RSMINEKLRKRIREEFRIQKNLFHRNIVEVYGIINTISFIAIFMECLTRGDLFDMI 508
Y++ IG+G V AQ+ GK +AVK++ ++ +N +++ E RI K + H NIV+++ +I + + + ME +RG++FD +
Sbjct: 21 YKLIKTIGKGNFAKVKLAQHVTTGKKVAVKVIDKTALNNSSLQKLFREVRIMKTIDHPNIVKLFEVIESERHVYLVMEYASRGEVFDYL 109 The following BLAST results are available for this feature:
BLAST of Protein kinase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of Protein kinase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30011043 ID=SMED30011043|Name=Protein kinase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1563bpback to top protein sequence of SMED30011043-orf-1 >SMED30011043-orf-1 ID=SMED30011043-orf-1|Name=SMED30011043-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=313bp MDIKPENVMISGSGVPKLIDFGLAVDHSVIPTIKGRLGTTAYSAPETFLDback to top protein sequence of SMED30011043-orf-2 >SMED30011043-orf-2 ID=SMED30011043-orf-2|Name=SMED30011043-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=156bp MTIPELDFERVHKELMQHLKSEIEPKLPNSYEISNMIGEGAIGVVYTAQNback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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