dynactin subunit 3

Overview
Namedynactin subunit 3
Smed IDSMED30010984
Length (bp)675
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of dynactin subunit 3 (SMED30010984) t-SNE clustered cells

Violin plots show distribution of expression levels for dynactin subunit 3 (SMED30010984) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of dynactin subunit 3 (SMED30010984) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for dynactin subunit 3 (SMED30010984) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010984

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
posterior sideSMED30010984h1SMcG0012976 dd_Smed_v4_7456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30010984h1SMcG0012976 dd_Smed_v4_7456_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Human
Match: DCTN3 (dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:2713])

HSP 1 Score: 55.0694 bits (131), Expect = 6.508e-9
Identity = 32/130 (24.62%), Postives = 68/130 (52.31%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            +S  R ++  +  +++++ KY+DP + D I++ +  K++ ILA+E+ I      L+Q+  L  +L S H+K       + + L+  +I  +++     E  + LL+EY +    +S   + W++ L   E
Sbjct:   47 ISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQDQCVEITEESKALLEEYNKTTMLLSKQFVQWDELLCQLE 176
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Human
Match: DCTN3 (dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:2713])

HSP 1 Score: 45.8246 bits (107), Expect = 7.544e-6
Identity = 21/71 (29.58%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLK 406
            +S  R ++  +  +++++ KY+DP + D I++ +  K++ ILA+E+ I      L+Q+  L  +L S H+K
Sbjct:   47 ISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIK 117
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Human
Match: DCTN3 (dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:2713])

HSP 1 Score: 46.2098 bits (108), Expect = 8.545e-6
Identity = 21/71 (29.58%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLK 406
            +S  R ++  +  +++++ KY+DP + D I++ +  K++ ILA+E+ I      L+Q+  L  +L S H+K
Sbjct:   47 ISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIK 117
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Human
Match: DCTN3 (dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:2713])

HSP 1 Score: 46.2098 bits (108), Expect = 8.968e-6
Identity = 21/71 (29.58%), Postives = 43/71 (60.56%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLK 406
            +S  R ++  +  +++++ KY+DP + D I++ +  K++ ILA+E+ I      L+Q+  L  +L S H+K
Sbjct:   47 ISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIK 117
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Zebrafish
Match: dctn3 (dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-65])

HSP 1 Score: 71.2478 bits (173), Expect = 6.557e-15
Identity = 40/140 (28.57%), Postives = 79/140 (56.43%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKL 571
            C   L  +S  +   +  R ++  +  +++++ KY+DP F D I++ ++ K+E ILA+EE +R  A  L+Q+  ++ +L S H+K    L  K + LS  +I  ++++E  +  V+ L +EY ++M  +S     W++ L
Sbjct:   35 CAESLTRISAALANTANKRERVKILHKKIEDLLKYLDPQFTDFIAVPDSVKLEFILAEEEFLRSQAALLEQVQNMQPLLDSNHIKAVPELTTKVQRLSQIHIQQQDQNEDLSAEVKRLFEEYNKMMFLLSKQFSQWDETL 174
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Zebrafish
Match: dctn3 (dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-65])

HSP 1 Score: 66.6254 bits (161), Expect = 2.898e-13
Identity = 36/126 (28.57%), Postives = 72/126 (57.14%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELM 529
            C   L  +S  +   +  R ++  +  +++++ KY+DP F D I++ ++ K+E ILA+EE +R  A  L+Q+  ++ +L S H+K    L  K + LS  +I  ++++E  +  V+ L +EY ++ 
Sbjct:   35 CAESLTRISAALANTANKRERVKILHKKIEDLLKYLDPQFTDFIAVPDSVKLEFILAEEEFLRSQAALLEQVQNMQPLLDSNHIKAVPELTTKVQRLSQIHIQQQDQNEDLSAEVKRLFEEYNKMF 160
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Mouse
Match: Dctn3 (dynactin 3 [Source:MGI Symbol;Acc:MGI:1859251])

HSP 1 Score: 49.2914 bits (116), Expect = 4.932e-7
Identity = 29/130 (22.31%), Postives = 65/130 (50.00%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            ++  R ++  +  +++++ KY+DP + D I++    K++ ILA+E+ I      L+Q+  L  +L S  +K       + + L+  +I  +++     E  + LL+ Y +    +S   + W++ L   E
Sbjct:   47 IASKRERVKILYKKIEDLIKYLDPEYIDRIAIPEASKLQFILAEEQFILSQVALLEQVNALVPVLDSASIKAVPEHAARLQRLAQIHIQQQDQCVAITEESKALLEGYNKTTMLLSKQFVQWDELLCQLE 176
[Close]
BLAST of dynactin subunit 3 vs. UniProt/SwissProt
Match: sp|Q0P5A1|DCTN3_BOVIN (Dynactin subunit 3 OS=Bos taurus OX=9913 GN=DCTN3 PE=2 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 2.802e-8
Identity = 32/130 (24.62%), Postives = 68/130 (52.31%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            ++  R ++  +  +++++ KY+DP + D I+L +  K++ ILA+E+ I      L+Q+  L  +L S H+K       + + L+  +I  +++     E  + LL+EY +    +S   + W++ L   E
Sbjct:   47 IASKRERVKVLYKKIEDLIKYLDPEYIDRIALPDASKLQFILAEEQFILSQVALLEQVEALVPMLDSTHIKAVPEHAARLQRLAQIHIQQQDQCVEITEESKALLEEYNKTTMLLSKQFVQWDELLCQLE 176
[Close]
BLAST of dynactin subunit 3 vs. UniProt/SwissProt
Match: sp|O75935|DCTN3_HUMAN (Dynactin subunit 3 OS=Homo sapiens OX=9606 GN=DCTN3 PE=1 SV=1)

HSP 1 Score: 55.0694 bits (131), Expect = 3.125e-8
Identity = 32/130 (24.62%), Postives = 68/130 (52.31%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            +S  R ++  +  +++++ KY+DP + D I++ +  K++ ILA+E+ I      L+Q+  L  +L S H+K       + + L+  +I  +++     E  + LL+EY +    +S   + W++ L   E
Sbjct:   47 ISSKRERVKILYKKIEDLIKYLDPEYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQDQCVEITEESKALLEEYNKTTMLLSKQFVQWDELLCQLE 176
[Close]
BLAST of dynactin subunit 3 vs. UniProt/SwissProt
Match: sp|Q9Z0Y1|DCTN3_MOUSE (Dynactin subunit 3 OS=Mus musculus OX=10090 GN=Dctn3 PE=1 SV=2)

HSP 1 Score: 49.2914 bits (116), Expect = 3.450e-6
Identity = 29/130 (22.31%), Postives = 65/130 (50.00%), Query Frame = 2 
Query:  194 VSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            ++  R ++  +  +++++ KY+DP + D I++    K++ ILA+E+ I      L+Q+  L  +L S  +K       + + L+  +I  +++     E  + LL+ Y +    +S   + W++ L   E
Sbjct:   47 IASKRERVKILYKKIEDLIKYLDPEYIDRIAIPEASKLQFILAEEQFILSQVALLEQVNALVPVLDSASIKAVPEHAARLQRLAQIHIQQQDQCVAITEESKALLEGYNKTTMLLSKQFVQWDELLCQLE 176
[Close]
BLAST of dynactin subunit 3 vs. TrEMBL
Match: A0A075AHA6 (UBIQUITIN_CONJUGAT_2 domain-containing protein (Fragment) OS=Opisthorchis viverrini OX=6198 GN=T265_13346 PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 4.321e-18
Identity = 48/130 (36.92%), Postives = 78/130 (60.00%), Query Frame = 2 
Query:  200 ENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKEKE 589
            E RP I K+ SR+ E++KY DP F DD S+S   K+E+IL ++E +  +A  L+++ +L  +L+    +D   L++K  EL+ +Y++ K  SE      Q LL  Y +L+   S L++ WNQK++S   E
Sbjct:  322 ERRPMISKMFSRLTELQKYADPHFVDDDSMSTKAKVEIILLEKEKLENMAVALEKIRQLSNVLNHPSFRDLASLRRKLNELNLTYVNQKQRSEQLITASQTLLANYYDLILATSKLLIQWNQKVVSTADE 451
[Close]
BLAST of dynactin subunit 3 vs. TrEMBL
Match: A0A1S8X6L8 (Ubiquitin--protein ligase OS=Opisthorchis viverrini OX=6198 GN=X801_01710 PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 4.739e-18
Identity = 48/130 (36.92%), Postives = 78/130 (60.00%), Query Frame = 2 
Query:  200 ENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKEKE 589
            E RP I K+ SR+ E++KY DP F DD S+S   K+E+IL ++E +  +A  L+++ +L  +L+    +D   L++K  EL+ +Y++ K  SE      Q LL  Y +L+   S L++ WNQK++S   E
Sbjct:  325 ERRPMISKMFSRLTELQKYADPHFVDDDSMSTKAKVEIILLEKEKLENMAVALEKIRQLSNVLNHPSFRDLASLRRKLNELNLTYVNQKQRSEQLITASQTLLANYYDLILATSKLLIQWNQKVVSTADE 454
[Close]
BLAST of dynactin subunit 3 vs. TrEMBL
Match: R7UQ88 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_189188 PE=4 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.254e-17
Identity = 42/137 (30.66%), Postives = 84/137 (61.31%), Query Frame = 2 
Query:  119 GDSVIPESFEPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELM 529
            GD+     +   ++ L     ++KK  E RPKI  ++ R+ ++E  +DP   + + LS + K ++ILA+E+ IRQ A NL ++  +++IL S+H+K    LQ+K  +LS+ ++  ++ +   +E+V++LL++Y  ++
Sbjct:   21 GDADKEADYPKIVDTLGKFDSQMKKAVEGRPKINTVIKRLDQLESLLDPAQMESLMLSQDAKADIILAEEQLIRQQAENLQKIQGMQDILGSEHIKAVPDLQEKLHQLSSVHLKQQDSTVALSEDVKDLLRQYNSIV 157
[Close]
BLAST of dynactin subunit 3 vs. TrEMBL
Match: A0A267G2M7 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009512g2 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.441e-16
Identity = 46/148 (31.08%), Postives = 87/148 (58.78%), Query Frame = 2 
Query:  146 EPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLK--DSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            E C   L  V+K+ +  +  R K+ +I++R  EI+KYMD  F ++ +LS   K+ +IL++E+ IR+ A  L+++++LK +L S+HL+  D  +L+ +F EL    ++   +++   +    LL  Y   M+ +S   + WN+K+ + E
Sbjct:   25 ESCAETLQTVNKKYEASTAKRDKVQRILARFNEIDKYMDSRFCEETALSTEAKIRVILSEEQQIRETAAALERISELKSVLDSEHLRAADQKLLE-RFAELRQKQLEQTEDAQKLEQQTYELLNVYFSFMDLVSKQFIAWNKKVRAAE 171
[Close]
BLAST of dynactin subunit 3 vs. TrEMBL
Match: A0A267ELX8 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009512g1 PE=4 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 1.521e-16
Identity = 46/148 (31.08%), Postives = 87/148 (58.78%), Query Frame = 2 
Query:  146 EPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLK--DSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            E C   L  V+K+ +  +  R K+ +I++R  EI+KYMD  F ++ +LS   K+ +IL++E+ IR+ A  L+++++LK +L S+HL+  D  +L+ +F EL    ++   +++   +    LL  Y   M+ +S   + WN+K+ + E
Sbjct:   26 ESCAETLQTVNKKYEASTAKRDKVQRILARFNEIDKYMDSRFCEETALSTEAKIRVILSEEQQIRETAAALERISELKSVLDSEHLRAADQKLLE-RFAELRQKQLEQTEDAQKLEQQTYELLNVYFSFMDLVSKQFIAWNKKVRAAE 172
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Cavefish
Match: dctn3 (dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-65])

HSP 1 Score: 60.4622 bits (145), Expect = 4.619e-11
Identity = 40/140 (28.57%), Postives = 76/140 (54.29%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKL 571
            C   L  ++  +   +  R ++ KI+ + +++ KY+DP F   I++ +  K+E ILA+E+ +   A  L+Q+  L+ +L S H+K    L  K + LS  +I    +SE  +  V+ L++EY ++M  +S     W++ L
Sbjct:   33 CAESLARINTALANTANKRERV-KILHKKEDLLKYLDPQFTAHIAVPDAMKLEFILAEEDFLCSQAALLEQVNNLQPLLDSNHIKVPE-LTTKVQRLSQIHIKQNGQSEELSAEVRRLIEEYNKMMFLLSKQFTQWDETL 170
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Cavefish
Match: dctn3 (dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-65])

HSP 1 Score: 53.5286 bits (127), Expect = 1.239e-8
Identity = 35/140 (25.00%), Postives = 72/140 (51.43%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKL 571
            C   L  ++  +   +  R ++  +  +++++ KY+DP F   I++ +  K+E ILA+E+ +   A  L+Q+  L+ +L S H+K   +    FR    + +      E+  E V+ L++EY ++M  +S     W++ L
Sbjct:   37 CAESLARINTALANTANKRERVKILHKKIEDLLKYLDPQFTAHIAVPDAMKLEFILAEEDFLCSQAALLEQVNNLQPLLDSNHIKGKMMGFSNFRCSRPAGVHICRLEELSAE-VRRLIEEYNKMMFLLSKQFTQWDETL 175
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Nematostella
Match: EDO44294 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RWD0])

HSP 1 Score: 64.3142 bits (155), Expect = 7.920e-13
Identity = 36/142 (25.35%), Postives = 73/142 (51.41%), Query Frame = 2 
Query:  146 EPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKL 571
            E C++ +  V + +K++S    ++ +   +VKE+E ++ P F +  +LS++ K ++I+  E  +      L ++  L +I++++HLKD  V   K   L    I  +      NE + NLL  Y  ++  +S   ++W+  L
Sbjct:   36 ESCLSTVAHVQQELKRLSSKYSRVAEAWKKVKELETFLSPEFVEGATLSDDVKADIIITGENKLISCTEKLSEIEDLNKIVNTEHLKDLPVWCAKMEPLIQVQIKQQEHVGEMNERLTNLLSCYNNIITTLSKQFIEWDALL 177
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Medaka
Match: dctn3 (dynactin subunit 3 [Source:NCBI gene;Acc:101164183])

HSP 1 Score: 69.707 bits (169), Expect = 4.536e-14
Identity = 38/144 (26.39%), Postives = 80/144 (55.56%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            C   L  +   +   +  R ++  +  +++++ KY+DP F D I++ +  K+E ILA+E+S+   A  L+Q++ L+ +L S +++D      K   LS  +I  ++++E  ++ V+ L +EY ++M  +S     W++ L + E
Sbjct:   90 CAESLARIQSALTNTANKRERVKILHKKIEDLMKYLDPQFTDHITVPDTIKLEFILAEEDSLLSQAALLEQVSSLQPLLDSTYIRDVPEHATKLHRLSQIHIKEQDQTEAQSQEVKKLFEEYNKMMFLLSKQFTQWDESLRTME 233
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Medaka
Match: dctn3 (dynactin subunit 3 [Source:NCBI gene;Acc:101164183])

HSP 1 Score: 69.707 bits (169), Expect = 4.536e-14
Identity = 38/144 (26.39%), Postives = 80/144 (55.56%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            C   L  +   +   +  R ++  +  +++++ KY+DP F D I++ +  K+E ILA+E+S+   A  L+Q++ L+ +L S +++D      K   LS  +I  ++++E  ++ V+ L +EY ++M  +S     W++ L + E
Sbjct:   90 CAESLARIQSALTNTANKRERVKILHKKIEDLMKYLDPQFTDHITVPDTIKLEFILAEEDSLLSQAALLEQVSSLQPLLDSTYIRDVPEHATKLHRLSQIHIKEQDQTEAQSQEVKKLFEEYNKMMFLLSKQFTQWDESLRTME 233
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Medaka
Match: dctn3 (dynactin subunit 3 [Source:NCBI gene;Acc:101164183])

HSP 1 Score: 69.707 bits (169), Expect = 4.536e-14
Identity = 38/144 (26.39%), Postives = 80/144 (55.56%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKE 583
            C   L  +   +   +  R ++  +  +++++ KY+DP F D I++ +  K+E ILA+E+S+   A  L+Q++ L+ +L S +++D      K   LS  +I  ++++E  ++ V+ L +EY ++M  +S     W++ L + E
Sbjct:   90 CAESLARIQSALTNTANKRERVKILHKKIEDLMKYLDPQFTDHITVPDTIKLEFILAEEDSLLSQAALLEQVSSLQPLLDSTYIRDVPEHATKLHRLSQIHIKEQDQTEAQSQEVKKLFEEYNKMMFLLSKQFTQWDESLRTME 233
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Medaka
Match: dctn3 (dynactin subunit 3 [Source:NCBI gene;Acc:101164183])

HSP 1 Score: 53.9138 bits (128), Expect = 1.390e-8
Identity = 28/102 (27.45%), Postives = 56/102 (54.90%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYI 457
            C   L  +   +   +  R ++  +  +++++ KY+DP F D I++ +  K+E ILA+E+S+   A  L+Q++ L+ +L S +++D      K   LS  +I
Sbjct:   90 CAESLARIQSALTNTANKRERVKILHKKIEDLMKYLDPQFTDHITVPDTIKLEFILAEEDSLLSQAALLEQVSSLQPLLDSTYIRDVPEHATKLHRLSQIHI 191
[Close]
BLAST of dynactin subunit 3 vs. Ensembl Medaka
Match: dctn3 (dynactin subunit 3 [Source:NCBI gene;Acc:101164183])

HSP 1 Score: 53.9138 bits (128), Expect = 1.390e-8
Identity = 28/102 (27.45%), Postives = 56/102 (54.90%), Query Frame = 2 
Query:  152 CINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYI 457
            C   L  +   +   +  R ++  +  +++++ KY+DP F D I++ +  K+E ILA+E+S+   A  L+Q++ L+ +L S +++D      K   LS  +I
Sbjct:   90 CAESLARIQSALTNTANKRERVKILHKKIEDLMKYLDPQFTDHITVPDTIKLEFILAEEDSLLSQAALLEQVSSLQPLLDSTYIRDVPEHATKLHRLSQIHI 191
[Close]
BLAST of dynactin subunit 3 vs. Planmine SMEST
Match: SMESG000010771.1 (SMESG000010771.1)

HSP 1 Score: 316.235 bits (809), Expect = 4.847e-111
Identity = 158/159 (99.37%), Postives = 159/159 (100.00%), Query Frame = 2 
Query:  119 GDSVIPESFEPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKEKENS 595
            GDSVIPESFEPCINQL+AVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKEKENS
Sbjct:   21 GDSVIPESFEPCINQLNAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNLDQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLLKEYTELMENMSLLVLDWNQKLISKEKENS 179
[Close]
BLAST of dynactin subunit 3 vs. Planmine SMEST
Match: SMESG000066339.1 (SMESG000066339.1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.815e-24
Identity = 48/79 (60.76%), Postives = 56/79 (70.89%), Query Frame = 2 
Query:  119 GDSVIPESFEPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANN 355
            G S I ES EPCI QLD V +RVK+VSEN P++  IV+ V E  K +DPTFFDDI L NND  E+IL DE+SIRQ   N
Sbjct:   21 GSSEILESLEPCIKQLDVVFERVKRVSENCPRVENIVNGVGETGKCIDPTFFDDIDLLNNDTFEIILVDEKSIRQRTKN 99
[Close]
BLAST of dynactin subunit 3 vs. Planmine SMEST
Match: SMESG000046803.1 (SMESG000046803.1)

HSP 1 Score: 93.9745 bits (232), Expect = 6.740e-23
Identity = 46/77 (59.74%), Postives = 57/77 (74.03%), Query Frame = 2 
Query:  119 GDSVIPESFEPCINQLDAVSKRVKKVSENRPKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIA 349
            G+SVI   FE CI+QLD V +RV +VSENRP++  I S V EIEK M+ TFF+ I LSNNDK++MI  DEESI Q+ 
Sbjct:  191 GNSVIRVRFESCISQLDVVFERVNRVSENRPRVRNIASGVGEIEKCMELTFFNGIGLSNNDKLKMIFVDEESICQMT 267
The following BLAST results are available for this feature:
BLAST of dynactin subunit 3 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4
Match NameE-valueIdentityDescription
DCTN36.508e-924.62dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:27... [more]
DCTN37.544e-629.58dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:27... [more]
DCTN38.545e-629.58dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:27... [more]
DCTN38.968e-629.58dynactin subunit 3 [Source:HGNC Symbol;Acc:HGNC:27... [more]
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BLAST of dynactin subunit 3 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynactin subunit 3 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynactin subunit 3 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dctn36.557e-1528.57dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-... [more]
dctn32.898e-1328.57dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-... [more]
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BLAST of dynactin subunit 3 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynactin subunit 3 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Dctn34.932e-722.31dynactin 3 [Source:MGI Symbol;Acc:MGI:1859251][more]
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BLAST of dynactin subunit 3 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 3
Match NameE-valueIdentityDescription
sp|Q0P5A1|DCTN3_BOVIN2.802e-824.62Dynactin subunit 3 OS=Bos taurus OX=9913 GN=DCTN3 ... [more]
sp|O75935|DCTN3_HUMAN3.125e-824.62Dynactin subunit 3 OS=Homo sapiens OX=9606 GN=DCTN... [more]
sp|Q9Z0Y1|DCTN3_MOUSE3.450e-622.31Dynactin subunit 3 OS=Mus musculus OX=10090 GN=Dct... [more]
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BLAST of dynactin subunit 3 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A075AHA64.321e-1836.92UBIQUITIN_CONJUGAT_2 domain-containing protein (Fr... [more]
A0A1S8X6L84.739e-1836.92Ubiquitin--protein ligase OS=Opisthorchis viverrin... [more]
R7UQ882.254e-1730.66Uncharacterized protein OS=Capitella teleta OX=283... [more]
A0A267G2M71.441e-1631.08Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267ELX81.521e-1631.08Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
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BLAST of dynactin subunit 3 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
dctn34.619e-1128.57dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-... [more]
dctn31.239e-825.00dynactin 3 (p22) [Source:ZFIN;Acc:ZDB-GENE-040704-... [more]
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BLAST of dynactin subunit 3 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynactin subunit 3 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of dynactin subunit 3 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO442947.920e-1325.35Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of dynactin subunit 3 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
dctn34.536e-1426.39dynactin subunit 3 [Source:NCBI gene;Acc:101164183... [more]
dctn34.536e-1426.39dynactin subunit 3 [Source:NCBI gene;Acc:101164183... [more]
dctn34.536e-1426.39dynactin subunit 3 [Source:NCBI gene;Acc:101164183... [more]
dctn31.390e-827.45dynactin subunit 3 [Source:NCBI gene;Acc:101164183... [more]
dctn31.390e-827.45dynactin subunit 3 [Source:NCBI gene;Acc:101164183... [more]
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BLAST of dynactin subunit 3 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000010771.14.847e-11199.37SMESG000010771.1[more]
SMESG000066339.11.815e-2460.76SMESG000066339.1[more]
SMESG000046803.16.740e-2359.74SMESG000046803.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010984 ID=SMED30010984|Name=dynactin subunit 3|organism=Schmidtea mediterranea sexual|type=transcript|length=675bp
AAATTAAAAAATTATTTTTAAAAATTATTTGTTAAATTTTATTATTAAAT
TTTGTAAAATGGATTCAACTTTAATTTATCTGGAAAGTAGATTAGAGGAA
ATCGAAAGAATATTGATAGGAGATTCTGTAATTCCTGAAAGTTTCGAGCC
ATGTATTAATCAGCTTGACGCTGTATCTAAAAGAGTAAAAAAAGTATCAG
AAAATCGTCCGAAGATTGGAAAAATTGTTAGTAGAGTTAAAGAAATTGAA
AAATATATGGATCCTACGTTTTTTGATGATATTAGTTTGTCAAATAATGA
TAAAATGGAAATGATTCTTGCTGATGAAGAAAGTATTCGCCAAATAGCAA
ATAACCTGGATCAGCTAACTAAATTAAAAGAAATCCTTAGCAGTCAACAT
CTAAAAGATTCTGGCGTGTTACAAAAGAAATTTAGAGAATTATCAGCTTC
GTACATCGATACCAAAAATGAGTCAGAGGTTTTTAATGAAAATGTTCAAA
ATTTACTGAAAGAATACACAGAACTAATGGAAAACATGTCACTTTTAGTT
CTTGATTGGAATCAAAAATTGATATCTAAAGAAAAGGAGAATTCATAGAA
TCTTTTTTATGGTTTATAAACTAAATATGGTTTTTGGTTTGACTATAAAT
AATTGCTTGTGATATAATGTAATTT
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protein sequence of SMED30010984-orf-1

>SMED30010984-orf-1 ID=SMED30010984-orf-1|Name=SMED30010984-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=180bp
MDSTLIYLESRLEEIERILIGDSVIPESFEPCINQLDAVSKRVKKVSENR
PKIGKIVSRVKEIEKYMDPTFFDDISLSNNDKMEMILADEESIRQIANNL
DQLTKLKEILSSQHLKDSGVLQKKFRELSASYIDTKNESEVFNENVQNLL
KEYTELMENMSLLVLDWNQKLISKEKENS*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0061640cytoskeleton-dependent cytokinesis
GO:0006888ER to Golgi vesicle-mediated transport
GO:0007017microtubule-based process
GO:0007049cell cycle
GO:0007067mitotic nuclear division
GO:0019886antigen processing and presentation of exogenous peptide antigen via MHC class II
GO:0051301cell division
GO:0097711ciliary basal body docking
GO:0000086G2/M transition of mitotic cell cycle
GO:0000910cytokinesis
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0003001lateral region of the whole animal
Vocabulary: INTERPRO
TermDefinition
IPR009991DCTN3
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
GO:0005198structural molecule activity
Vocabulary: cellular component
TermDefinition
GO:0005694chromosome
GO:0005730nucleolus
GO:0005737cytoplasm
GO:0005813centrosome
GO:0005815microtubule organizing center
GO:0005819spindle
GO:0005829cytosol
GO:0005856cytoskeleton
GO:0005869dynactin complex
GO:0005875microtubule associated complex
GO:0030496midbody
GO:0032154cleavage furrow
GO:0048471perinuclear region of cytoplasm
GO:0000775chromosome, centromeric region
GO:0000776kinetochore
GO:0000777condensed chromosome kinetochore
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 139..163
NoneNo IPR availableCOILSCoilCoilcoord: 93..113
IPR009991Dynactin subunit 3PFAMPF07426Dynactin_p22coord: 7..168
e-value: 2.2E-38
score: 131.7
IPR009991Dynactin subunit 3PANTHERPTHR28360FAMILY NOT NAMEDcoord: 7..177