G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview


Name	G_PROTEIN_RECEP_F1_2 domain-containing protein
Smed ID	SMED30010971
Length (bp)	1182

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010971) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010971) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010971) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30010971

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication	Specimen	Lifecycle	Evidence
tail region	SMED30010971		dd_Smed_v6_17279_0_1	dd_Smed_v6	PMID:28171748 Stückemann et al., 2017

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A193KUG9 (GCR049 OS=Schmidtea mediterranea OX=79327 GN=gcr049 PE=2 SV=1)

HSP 1 Score: 148.288 bits (373), Expect = 4.029e-37
Identity = 114/341 (33.43%), Postives = 180/341 (52.79%), Query Frame = 1

Query:  106 ANCSNQTDDQTKEFSLN--YFLLSMIVTGINIIAHSFALAIISK-----PTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVR-IHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFESI----VIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKKPNSNNTR 1092
             NCS  + + +   ++   Y ++S+ +  IN  A+ FAL I SK     P       +M +I++  S IEL FQ+T+  F L EY ++D  II   ++    +D + R I+++ +  FL+ARN  I  LA HR+E IC P+ + +Y+T +   +    ++  ++L+ FP++FE    YC  TG V    + L+    YRI+Y N  +FL Q   P++ V  LSIL+L KI +   F R      + + +   +  D L+V LC+TF   EMP+FFSKL      I    +I  L  IANI IY+DS  N  +Y  SN+I +    ++ +K   K   + T+
Sbjct:    8 TNCSTNSGNFSASANIEKIYQIISITLLSINSFANCFALHIFSKFLWHMPI------LMNQILARFSFIELIFQMTLLLFYLVEYMKIDEYIISSRFVNPGIMDALFRCIYLFSVG-FLIARNSWIILLAFHRFECICYPLRTKRYVTSANINFYFFGIVSFAILIAFPRMFEQTYTYCLQTGDVVNVSEALSNVYQYRIVYLNVFVFLIQIGIPLLSVNILSILMLRKIRQSLTFRR---TSAAPNQKKKSFAADKLVVALCITFFTFEMPSFFSKLFHNSHIISNDKIIYHLTFIANISIYIDSSMNLLIYLVSNRIFRGAVLQQISKVSHKKTVSTTK 338

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A074YY29 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Opisthorchis viverrini OX=6198 GN=T265_11621 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 3.046e-26
Identity = 99/375 (26.40%), Postives = 175/375 (46.67%), Query Frame = 1

Query:  109 NCSNQTDDQTKEFSLNYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCE---------LDTIIIKYGYLEFYT---VDIIVRIHIYGIHV--------FLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHR-----------------KYFVRKSNSLRHAKWTT-----------------DVLIVFLCLTFLVLEMPAFFSKLCQMF----ESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAK 1059
            +C         +  L++ ++S+ +  +N   +   + I + P      S M R++  LS+IEL   +++F + L + C           ++I +    L+FYT    + +    I+ + +        F +ARN+ +  LA +RY+ +C+PIGS    T      I++ ++  S +L  P+ FE  II CH TG+      LL++  +Y+I Y   ++F+ Q  GPVI V  L+  I+  I +R +  R                 +   R ++S+++A++++                 D LI  +C+ F + E P+FFSK+ + +      +V   L++IANI IYLDS  N F+Y  SN   +   S K  K
Sbjct:   18 SCQPAPAHPPDKLRLSHGIISLTLGPLNAFLNGICVLIFTHPLW--KKSTMSRLLRGLSVIELCMGISLFAYALLDNCSSHLQTHFDLRESIPVGNTTLDFYTDRHRNYVKHTSIFVLSIWFSLCLLAFQVARNWSVVLLAAYRYDRLCRPIGSGSAFTDERICSILVAVVGCSCVLVIPRAFEAAIIICHRTGLFSGVKLLLSDNHIYQIAYLGVVMFIVQAGGPVICVCVLNAFIIRLIVKRRQLQREKEEKSALRLLNQRSISEVSERGNDSIQNARFSSISKPVNYHVPPKPPPSGDKLIFAVCVCFFIFETPSFFSKILRPYLENNYPVVDSWLSIIANILIYLDSTLNAFVYMASNPTFRKIVSEKLLK 390

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: G4VR07 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mansoni OX=6183 GN=Smp_204230 PE=4 SV=1)

HSP 1 Score: 119.783 bits (299), Expect = 3.511e-26
Identity = 102/365 (27.95%), Postives = 168/365 (46.03%), Query Frame = 1

Query:  157 YFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVF--------------------TFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKY-----------------------------------FVRKSNSL-----RHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMF----ESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAK 1059
            Y  + +++  +N I +   + I +   S    S+M RI+  LSIIEL   L++F                    T +++ Y      I    Y++      I+ I    + VF ++RN+ +  LA +RY+ IC+PIGS          YI+II+ F + L+  P+IFE  I+ C+ +G V +   LL  + +Y+I+Y   ++F+ Q+ GPVI V  LS  ++  I +R +FHR+                                      R SN L          + D L+  +C+TF +LE PAFFSK+   +      ++   L++IAN+ IYLDS  N F+Y  SN + +  +  +C +
Sbjct:   43 YRAIGLLLGPLNTILNGLCVHIFNH--SSWKKSIMSRILKGLSIIELGMGLSLFIHALIFSQNSTTTSHLHQNTTNSIYPYHNEQDTIQTNAYIKTIFSFFIMTISSRFLVVFQISRNWSVVLLAAYRYDQICRPIGSPSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCYISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNIINNYNNNPLKRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLEQDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFRKIAREECIQ 405

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3Q0KV19 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 4.104e-26
Identity = 102/363 (28.10%), Postives = 167/363 (46.01%), Query Frame = 1

Query:  157 YFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVF--------------------TFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKY-----------------------------------FVRKSNSL-----RHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMF----ESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKC 1053
            Y  + +++  +N I +   + I +   S    S+M RI+  LSIIEL   L++F                    T +++ Y      I    Y++      I+ I    + VF ++RN+ +  LA +RY+ IC+PIGS          YI+II+ F + L+  P+IFE  I+ C+ +G V +   LL  + +Y+I+Y   ++F+ Q+ GPVI V  LS  ++  I +R +FHR+                                      R SN L          + D L+  +C+TF +LE PAFFSK+   +      ++   L++IAN+ IYLDS  N F+Y  SN + +  +  +C
Sbjct:   42 YRAIGLLLGPLNTILNGLCVHIFNH--SSWKKSIMSRILKGLSIIELGMGLSLFIHALIFSQNSTTTSHLHQNTTNSIYPYHNEQDTIQTNAYIKTIFSFFIMTISSRFLVVFQISRNWSVVLLAAYRYDQICRPIGSPSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCYISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNIINNYNNNPLKRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLEQDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFRKIAREEC 402

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A183P9C9 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS10965 PE=4 SV=1)

HSP 1 Score: 114.775 bits (286), Expect = 1.835e-24
Identity = 95/349 (27.22%), Postives = 163/349 (46.70%), Query Frame = 1

Query:  163 LLSMIVTGINIIAHSFALAII-------SKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRK----------------------------------YFVRKSNSL-----RHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMF----ESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAK 1059
            ++S I+ G++II     L++           TS  +N  +  I +  + I   + + +   N F++  + T         F+   I  R  +     F ++RN+ +  LA +RY+ IC+PIG+S         YI+II+ F + L+  P+IFE  I+ C+ +G V +   LL  + +Y+I+Y   ++F+ Q+ GPVI V  LS  ++  I +R +FHR+                                  +  R SN L          + D L+  +C+TF +LE PAFFSK+   +      ++   L++IAN+ IYLDS  N F+Y  SN + +  +  +C +
Sbjct:   73 IMSRILKGLSIIELGMGLSLFIHALIFSQNSTSSYSNQNLFNINNTTNSIYPNYDIHINHLNQFQHDSIHTNAYIKTICSFFITTISSRFLV----AFQISRNWSVVLLAAYRYDQICRPIGTSSAFPRERIRYILIIIFFLACLIVIPRIFESGIVVCYISGNVSKDYPLLINYKLYQILYLGLVMFIVQSGGPVICVCVLSAFVIRIIAKRRKFHREKEQRSARNLLRRQTMEIENNQSNLKNNTNNNNTNFLQRNSNGLTKELTERPAPSGDKLMFAVCITFFILETPAFFSKILNPYLEQDYPLIDLTLSVIANLLIYLDSTLNAFIYMASNPLFRKIARNECIQ 417

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000045974.1 (SMESG000045974.1)

HSP 1 Score: 700.279 bits (1806), Expect = 0.000e+0
Identity = 359/360 (99.72%), Postives = 359/360 (99.72%), Query Frame = 1

Query:   82 MYLASILYANCSNQTDDQTKEFSLNYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKKPNSNNTRLEMLNGKMSSSDHCKIFNQIVQS 1161
            MYLASILYANCSNQTDDQTKEFSLNYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASL LTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVIIVITLSILILIKIHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKKPNSNNTRLEMLNGKMSSSDHCKIFNQIVQS
Sbjct:    1 MYLASILYANCSNQTDDQTKEFSLNYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLFLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVIIVITLSILILIKIHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKKPNSNNTRLEMLNGKMSSSDHCKIFNQIVQS 360

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000004763.1 (SMESG000004763.1)

HSP 1 Score: 148.288 bits (373), Expect = 1.642e-40
Identity = 115/341 (33.72%), Postives = 180/341 (52.79%), Query Frame = 1

Query:  106 ANCSNQTDDQTKEFSLN--YFLLSMIVTGINIIAHSFALAIISK-----PTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVR-IHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFESI----VIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKKPNSNNTR 1092
             NCS  + + +   ++   Y ++S+ +  IN  A+ FAL I SK     P       +M +I++  S IEL FQ+T+  F L EY ++D  II   ++    +D + R I+++ +  FL+ARN  I  LA HR+E IC P+ + +Y+T +   +    ++  ++L+ FP++FE    YC  TG V    + L+    YRIIY N  +FL Q   P++ V  LSIL+L KI +   F R      + + +   +  D L+V LC+TF   EMP+FFSKL      I    +I  L  IANI IY+DS  N  +Y  SN+I +    ++ +K   K   + T+
Sbjct:   29 TNCSTNSGNFSASANIEKIYQIISITLLSINSFANCFALHIFSKFLWHMPI------LMNQILARFSFIELIFQMTLLLFYLVEYIKIDEYIISSRFVNPGIMDALFRCIYLFSVG-FLIARNSWIILLAFHRFECICYPLRTKRYVTSANINFYFFGIVSFAILIAFPRMFEQTYTYCLQTGDVVNVSEALSNVYQYRIIYLNVFVFLIQIGIPLLSVNILSILMLRKIRQSLTFRR---TSAAPNQKKKSFAADKLVVALCITFFTFEMPSFFSKLFHNSHIISNDKIIYHLTFIANISIYIDSSMNLLIYLVSNRIFRGAVLQQISKVSHKKTVSTTK 359

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000022454.1 (SMESG000022454.1)

HSP 1 Score: 113.235 bits (282), Expect = 5.637e-28
Identity = 94/323 (29.10%), Postives = 160/323 (49.54%), Query Frame = 1

Query:  109 NCSNQTDDQTKEFSLNYFLL------SMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVR--IHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNT-GMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQMFES-----IVIERLAMIANICIYLDSFANFFLYFFSNKILKM 1035
            NCS    + +   S +  L       S++++ I+ + ++ A+ I +        S+   I+  LS+ E  F  TV   N ++   + +    +   E   +D   R  I ++GI VFL+ RN  +  LA HRYE +C P+G+ + I       I +++   +L++  P++FE +++ C      +  ++  L+    Y  ++   ++F+ Q+ GPVI V  LS  IL  I +  +F      R SN+    +   D +I  LCL F +LE P+FFSK+ + +       IV   L ++ANICIY+DS  NFF+Y  SN   K+
Sbjct:    7 NCSKIITNASGHVSYSNLLFTYYNYSSIVLSSISFLLNTLAIFIFNHRLW--RKSLTSIILKYLSVFESLFNATVLIKNSYDNYAIQSKSPNFSSYESLLIDETFRLFIRLFGI-VFLICRNVTVAVLAAHRYECVCLPVGAKRRINSRNVFQIELVIFLLALVIALPRLFEKYVVACAEPLWKIEGKVAFLDSSNWYVYLHKGVVLFIFQSGGPVITVFILSFYILQVIKKMKKFRN----RNSNNKVRPQPLGDKIIFPLCLIFFILESPSFFSKILEAYLRSKNLVIVDHTLLVVANICIYVDSIINFFIYIISNPTFKL 322

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000076138.1 (SMESG000076138.1)

HSP 1 Score: 110.923 bits (276), Expect = 4.877e-27
Identity = 93/327 (28.44%), Postives = 166/327 (50.76%), Query Frame = 1

Query:  109 NCSNQTDDQTKEFSLNYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPI------GSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLC-QMFESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSR--KCAKS 1062
            NCS+  + +     + Y  ++ ++   NI+ + FA+ I+S   S  N S++  I+  +S  E+ F L  F  N+ +  E       +G     TV+I++ I    +  F +ARN+ +  LA+ RY+++C+          S + +    P I   ++   + L  P++FE     C NT ++      L+   V++  Y   ++F+ Q+ GP+II   +S++++ KI  R    ++     SN  + ++     L++ +C+TF+VLE P+FFSKL    F++  I  + +I N+ IYLDS  N F+Y  SNK  +  S    +CAKS
Sbjct:   23 NCSSCLEKKNG-MPITYTYITFVLVVTNILGNMFAVIILSD--SVWNKSVLTTILKAISYFEILFNLLNFINNICQLTEPLKPFQYFGLKALATVEILLLICDSLMIGFQMARNWSVTILALFRYDMVCRSKTVRRLNNQSNFFSNENLPKIFTFLMIFCIGLALPRMFEVTPQICINTAVICHYADFLSVRKVFQYGYLGVVMFIFQSGGPIIITSCVSLMLIRKIQNRHAIRKRQTNATSNRFQRSQRNPGTLVMAVCITFMVLEFPSFFSKLAFYAFDNNNIH-IGIIPNLLIYLDSCLNLFIYLASNKTFRKTSLNRIRCAKS 345

[Close]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000023730.1 (SMESG000023730.1)

HSP 1 Score: 111.694 bits (278), Expect = 1.424e-26
Identity = 97/323 (30.03%), Postives = 171/323 (52.94%), Query Frame = 1

Query:  106 ANCSNQTDDQTKEFSL------NYFLLSMIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFTFNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLAIHRYEVICKPIGSSK-YITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCHNTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVXXXXXXXXXXXXXXHRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQ---MFESIVIER--LAMIANICIYLDSFANFFLYFFSNKILKMK 1038
             N S+++D+    F+L      NY LL  ++  I+II + F L I          S++  ++  LS  E+ F ++ F +++ ++ +  +I     + + + V  I++I  + +   L+ RN+ I  LAI+R++ IC+P+ + K Y   ST+  + I  I A+ ++ FP+ FE    +C   G +  +  LLN +  Y   Y  ++IF+ QTA PV+    LSI ++  I +   F + +   K+   ++   + D L++F+CL F + E P+FFSK  Q   M  + +IE   LA +AN+ +YLDS  N F+Y  SN + + K
Sbjct:  247 GNKSDESDENLAAFALRIYNFHNYALL--VIPIISIIFNGFCLFIFFDKIW--KKSILSILLKYLSATEIVFNISGFLYSVTKFIDYPSI---NQFSKLFWV-FILQIGTFSVASGLICRNWTILHLAIYRFDSICRPLNTKKIYTETSTYKLLWITYILAA-VIAFPRTFEYKATFCE--GNLVLKPSLLN-YDWYAYGYQGSLIFIIQTAAPVVCTSILSISVMRAILKVKRFRKSF--SKTIKTKNNAISGDALMIFICLAFFLFETPSFFSKALQSYLMRNNKIIEDVLLATVANLLVYLDSVINIFVYILSNPVFRKK 555

The following BLAST results are available for this feature:

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A193KUG9	4.029e-37	33.43	GCR049 OS=Schmidtea mediterranea OX=79327 GN=gcr04...	[more]
A0A074YY29	3.046e-26	26.40	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]
G4VR07	3.511e-26	27.95	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]
A0A3Q0KV19	4.104e-26	28.10	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]
A0A183P9C9	1.835e-24	27.22	G_PROTEIN_RECEP_F1_2 domain-containing protein OS=...	[more]

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5


Match Name	E-value	Identity	Description
SMESG000045974.1	0.000e+0	99.72	SMESG000045974.1	[more]
SMESG000004763.1	1.642e-40	33.72	SMESG000004763.1	[more]
SMESG000022454.1	5.637e-28	29.10	SMESG000022454.1	[more]
SMESG000076138.1	4.877e-27	28.44	SMESG000076138.1	[more]
SMESG000023730.1	1.424e-26	30.03	SMESG000023730.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30010971 ID=SMED30010971|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1182bp
TTCGAATAAAAATTCTATTGGATGATCTCAATATTCTGTTATAAAATTTA
TGAAGTTAATTTTCTAAGAGGAATTATTAAAATGTATTTGGCATCCATCT
TGTATGCTAACTGCAGCAATCAAACAGATGATCAAACGAAGGAATTTTCA
CTTAACTACTTTTTATTATCAATGATTGTAACAGGAATAAACATTATTGC
TCATTCATTTGCATTGGCTATTATTTCCAAACCGACAAGTGGTTCAAATA
ACAGCATGATGATGAGAATTATTTCCAATTTGAGCATTATTGAATTGTTT
TTTCAATTGACGGTATTTACTTTCAATTTGTTTGAATATTGCGAATTGGA
TACAATTATCATCAAATATGGATATCTGGAATTTTATACTGTCGATATCA
TAGTGAGGATTCATATTTATGGAATTCATGTTTTTCTACTTGCCCGAAAT
TTTATCATTTGTTCACTGGCTATTCATAGATACGAAGTGATTTGCAAACC
AATCGGATCCAGTAAATACATTACATTTTCAACATGGCCGTATATTATTA
TAATTATGATTTTCGCGTCTCTCTTACTTACGTTTCCAAAAATCTTCGAG
GATCATATAATTTATTGCCATAATACAGGAATGGTTTTCCAAAGGATGAA
ATTACTCAATGAATTTATCGTTTATAGAATAATCTATACCAATACTATAA
TATTTCTTACCCAAACTGCAGGTCCAGTGATAATTGTTATAACTTTGAGT
ATACTTATTTTAATCAAAATTCACAGAAGAACTGAGTTTCATAGGAAATA
TTTCGTCCGAAAATCAAATTCACTGCGCCATGCAAAGTGGACTACGGACG
TTTTAATCGTTTTTCTTTGTCTAACCTTTCTAGTGTTGGAAATGCCAGCT
TTTTTCAGTAAATTATGTCAAATGTTTGAAAGTATAGTAATAGAAAGGTT
GGCAATGATTGCTAATATTTGTATATACTTGGATTCCTTTGCAAATTTTT
TCTTGTATTTCTTTTCAAATAAAATACTTAAAATGAAATCGTCAAGAAAA
TGCGCCAAATCTCCTAAGAAACCAAATAGCAATAATACGAGATTGGAAAT
GTTAAATGGCAAAATGAGTTCGTCTGATCATTGTAAGATTTTTAATCAAA
TTGTACAATCTTGAAATATATTATTTAGCGGC

protein sequence of SMED30010971-orf-1

>SMED30010971-orf-1 ID=SMED30010971-orf-1|Name=SMED30010971-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp
MISIFCYKIYEVNFLRGIIKMYLASILYANCSNQTDDQTKEFSLNYFLLS
MIVTGINIIAHSFALAIISKPTSGSNNSMMMRIISNLSIIELFFQLTVFT
FNLFEYCELDTIIIKYGYLEFYTVDIIVRIHIYGIHVFLLARNFIICSLA
IHRYEVICKPIGSSKYITFSTWPYIIIIMIFASLLLTFPKIFEDHIIYCH
NTGMVFQRMKLLNEFIVYRIIYTNTIIFLTQTAGPVIIVITLSILILIKI
HRRTEFHRKYFVRKSNSLRHAKWTTDVLIVFLCLTFLVLEMPAFFSKLCQ
MFESIVIERLAMIANICIYLDSFANFFLYFFSNKILKMKSSRKCAKSPKK
PNSNNTRLEMLNGKMSSSDHCKIFNQIVQS*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000421	tail
PLANA:0002089	reproductive organ

Vocabulary: **INTERPRO**
Term	Definition
IPR017452	GPCR_Rhodpsn_7TM

Vocabulary: **cellular component**
Term	Definition
GO:0016021	integral component of membrane
GO:0016020	membrane

Vocabulary: **molecular function**
Term	Definition
GO:0004930	G-protein coupled receptor activity

Vocabulary: **biological process**
Term	Definition
GO:0007186	G-protein coupled receptor signaling pathway

Main menu

G_PROTEIN_RECEP_F1_2 domain-containing protein

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 1

Owlery

blazegraph-runner