Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative

Overview
NameCore 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative
Smed IDSMED30010966
Length (bp)1102
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative (SMED30010966) t-SNE clustered cells

Violin plots show distribution of expression levels for Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative (SMED30010966) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative (SMED30010966) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative (SMED30010966) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010966

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30010966SMESG000061089.1 SmedASXL_012763SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
protonephridiaSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neuronSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		whole organism asexual adult colorimetric in situ hybridization evidence
cilated neuronSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010966SMESG000061089.1 Contig42285newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010966SMESG000061089.1 Contig42285uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
head regionSMED30010966SMESG000061089.1 dd_Smed_v6_2796_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
epidermisSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
ciliated epithelial cellSMED30010966SMESG000061089.1 dd_Smed_v4_2796_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Human
Match: AC008687.4 (novel protein)

HSP 1 Score: 103.219 bits (256), Expect = 2.931e-24
Identity = 90/303 (29.70%), Postives = 141/303 (46.53%), Query Frame = 3 
Query:  144 FNSTLRNPLPPVLAKPP------EILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPN--KWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNP-NSSYEVPRMYETEYASYGNDYPTIL 1025
              ST+ +PLP V  +        E+L    ++TT+G+ +  K   G   Q P         +   +KD  E + +RGWR PL+ +YQ SET  +++  P   +P   ++   Q   L +HH  GPS+ ++A T+NP+L GR     D+ VL     Y+TT+ +  R +   ELSG  +KD  TYW  EE P+V   GP+    P+S+ P   P +R   +    + +          PH G  +   + Y        +     P++ P   P   P    Y VP+ Y TE +SYG+  P ++
Sbjct:   46 VQSTVADPLPQVAKQDSHNWAFDEVL--SRWETTSGSAYVPKTHGGPCAQ-PRAPEPADPTRTVGIKDLGEKLRHRGWRLPLTTKYQSSETRAQYTGSPS-GDPRAPEYFGPQPPQLADHHRGGPSQALIAWTKNPELSGRPFTVSDRGVLDRRQLYLTTSARDFRFYPKTELSGYPRKDSLTYWSFEETPQVWSHGPQRPPCPRSSRPPRPPRVRVPRVSPVTSAM----------PHRGALSLAQESYSPLLHPLRRLDRFCPLEAPWGGPHWKPLRGIYSVPKAYSTENSSYGSLKPALV 334
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Mouse
Match: AC151602.1 (luteinizing hormone beta [Source:NCBI gene;Acc:16866])

HSP 1 Score: 58.151 bits (139), Expect = 1.887e-9
Identity = 54/196 (27.55%), Postives = 89/196 (45.41%), Query Frame = 3 
Query:   24 PMTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHN---KVHYLKDFMENIP-NRGWRRPLSPRYQKSETHDEFSNPPKLYEPN-KWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMT 596
            P + I+E  +    P   L  TS GV HHW P   FP     S ++ PLP V     +++    ++TT+ + +  K   G   Q     +AP H    +   +K   E +  + G   PL+ + Q SET  ++   P + +    + + Q   L +HH  GPS+ ++  T+NP++ G+        +L  L PY T
Sbjct:   13 PWSPISETEVLGTWPNWHL--TSSGVAHHWIPPASFPLPTLQSMVKAPLPAV----SKVI--SRWETTSQSTYTPKTHGGPCAQ----PKAPEHKDPRRTLGIKSLAEKLRRHEGRGVPLNTKCQISETKAQYKGWPDVDQSAPLFAEPQPPELADHHRGGPSQALIPWTKNPEMAGQPFTVNKMGILDRLQPYQT 196
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Mouse
Match: AC151602.1 (luteinizing hormone beta [Source:NCBI gene;Acc:16866])

HSP 1 Score: 57.7658 bits (138), Expect = 5.668e-9
Identity = 54/196 (27.55%), Postives = 89/196 (45.41%), Query Frame = 3 
Query:   24 PMTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHN---KVHYLKDFMENIP-NRGWRRPLSPRYQKSETHDEFSNPPKLYEPN-KWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMT 596
            P + I+E  +    P   L  TS GV HHW P   FP     S ++ PLP V     +++    ++TT+ + +  K   G   Q     +AP H    +   +K   E +  + G   PL+ + Q SET  ++   P + +    + + Q   L +HH  GPS+ ++  T+NP++ G+        +L  L PY T
Sbjct:   13 PWSPISETEVLGTWPNWHL--TSSGVAHHWIPPASFPLPTLQSMVKAPLPAV----SKVI--SRWETTSQSTYTPKTHGGPCAQ----PKAPEHKDPRRTLGIKSLAEKLRRHEGRGVPLNTKCQISETKAQYKGWPDVDQSAPLFAEPQPPELADHHRGGPSQALIPWTKNPEMAGQPFTVNKMGILDRLQPYQT 196
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Mouse
Match: AC151602.1 (luteinizing hormone beta [Source:NCBI gene;Acc:16866])

HSP 1 Score: 57.7658 bits (138), Expect = 5.668e-9
Identity = 54/196 (27.55%), Postives = 89/196 (45.41%), Query Frame = 3 
Query:   24 PMTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHN---KVHYLKDFMENIP-NRGWRRPLSPRYQKSETHDEFSNPPKLYEPN-KWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMT 596
            P + I+E  +    P   L  TS GV HHW P   FP     S ++ PLP V     +++    ++TT+ + +  K   G   Q     +AP H    +   +K   E +  + G   PL+ + Q SET  ++   P + +    + + Q   L +HH  GPS+ ++  T+NP++ G+        +L  L PY T
Sbjct:   13 PWSPISETEVLGTWPNWHL--TSSGVAHHWIPPASFPLPTLQSMVKAPLPAV----SKVI--SRWETTSQSTYTPKTHGGPCAQ----PKAPEHKDPRRTLGIKSLAEKLRRHEGRGVPLNTKCQISETKAQYKGWPDVDQSAPLFAEPQPPELADHHRGGPSQALIPWTKNPEMAGQPFTVNKMGILDRLQPYQT 196
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Match: A0A267E3Z0 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009082g2 PE=4 SV=1)

HSP 1 Score: 351.673 bits (901), Expect = 7.486e-117
Identity = 176/331 (53.17%), Postives = 224/331 (67.67%), Query Frame = 3 
Query:   27 MTSITERNITV---RLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDV-QNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGN 1007
            M++  ER++++   R+PTNDLF+TS GVGHHWRPGYYFPDS F +    PLP  L +P  + P + + TT+ + H+ K P   L +   FKRAP    VH+LKD  E + + GWRRPLSP +Q +ET +EF N P L EPN W    Q F L +HH +G +K  +AST+NPKLKGR L+PKD+EVLR LD Y+TTN + HR FDPKELSG AKKDI TYWDCEEYPK  G GP EN +PK A+PR+  PMRD   FKSAT++RR+P       H+GM+T Y D Y +PSD + KEA   P+D P  +P P   S+   P+MY+TEY S G+
Sbjct:    1 MSTTIERDLSMTAKRMPTNDLFQTSAGVGHHWRPGYYFPDSGFTAASTQPLPDELTRPSRVRPDELYTTTSASAHEPKRPTAPLNRDIEFKRAPALYNVHFLKDHKEKLGHGGWRRPLSPSHQLTETQEEFRNQPPLSEPNTWGSKPQPFLLRDHHGEGSTKLGVASTQNPKLKGRPLFPKDKEVLRGLDMYLTTNDRAHRRFDPKELSGYAKKDIATYWDCEEYPKAWGHGPHENLLPKHAVPRDRLPMRDELRFKSATSVRRVPQRLPPPAHTGMKTLYSDSYARPSDARVKEARECPVDTPWMLPAPGARSAMAAPQMYKTEYQSVGS 331
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Match: A0A1I8JLA9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig009082g1 PE=4 SV=1)

HSP 1 Score: 351.673 bits (901), Expect = 8.348e-117
Identity = 176/331 (53.17%), Postives = 224/331 (67.67%), Query Frame = 3 
Query:   27 MTSITERNITV---RLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDV-QNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGN 1007
            M++  ER++++   R+PTNDLF+TS GVGHHWRPGYYFPDS F +    PLP  L +P  + P + + TT+ + H+ K P   L +   FKRAP    VH+LKD  E + + GWRRPLSP +Q +ET +EF N P L EPN W    Q F L +HH +G +K  +AST+NPKLKGR L+PKD+EVLR LD Y+TTN + HR FDPKELSG AKKDI TYWDCEEYPK  G GP EN +PK A+PR+  PMRD   FKSAT++RR+P       H+GM+T Y D Y +PSD + KEA   P+D P  +P P   S+   P+MY+TEY S G+
Sbjct:    1 MSTTIERDLSMTDKRMPTNDLFQTSAGVGHHWRPGYYFPDSGFTAASTQPLPDELTRPSRVRPDELYTTTSASAHEPKRPTAPLNRDIEFKRAPALYNVHFLKDHKEKLGHGGWRRPLSPSHQLTETQEEFRNQPPLSEPNTWGSKPQPFLLRDHHGEGSTKLGVASTQNPKLKGRPLFPKDKEVLRGLDMYLTTNDRAHRRFDPKELSGYAKKDIATYWDCEEYPKAWGHGPHENLLPKHAVPRDRLPMRDELRFKSATSVRRVPQRLPPPAHTGMKTLYSDSYARPSDARVKEARECPVDTPWMLPAPGARSAMAAPQMYKTEYQSVGS 331
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Match: R7VGG9 (Uncharacterized protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_224565 PE=4 SV=1)

HSP 1 Score: 298.901 bits (764), Expect = 4.399e-96
Identity = 160/333 (48.05%), Postives = 206/333 (61.86%), Query Frame = 3 
Query:   45 RNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKY---PYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEF-SNPPKLYEPNKWLDV--QNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL 1025
            R   ++    + F+TS G GHHWRPGYYFP S F  T   PLPP L KP EI    H+KT+TGT HD K    PYG+  Q   +KRAP   KV Y+ D  + +   GW++PL+   Q SETHD F + P  L E  +   V  Q F L NHH +GPSKK++ +TEN K+KGR  Y K++ +L   DPY+T+    HR F PKEL+G  KKDI TYW CEEYPK  G G K N VPKS I +E PPMRD  +FK  T +RRIPP    VPH+G+QTEY + + KP DV+ KE   +P+D P  +P+    S++  P+MY+TEY + G++ P   
Sbjct:   10 RGTELKRDIYETFQTSTGKGHHWRPGYYFPHSNFQRTADCPLPPELVKPSEIDNAQHYKTSTGTAHDFKTTGGPYGNQSQE--YKRAPALYKVDYVNDHHDKLQAGGWKKPLTMGNQTSETHDRFRAEPDVLAERPRDFAVNPQGFVLSNHHTNGPSKKVLPTTENDKMKGREFYVKNKGILDLNDPYLTSTASAHRRFKPKELNGYPKKDIATYWGCEEYPKSWGHGLKHNPVPKSLILKEQPPMRDEMVFKFPTKVRRIPPITNHVPHAGLQTEYGNHFKKPGDVRCKEGEYTPVDTPFVLPEAGSKSTFAAPKMYKTEYTNIGSNRPITC 340
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Match: V4AK99 (Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_104414 PE=4 SV=1)

HSP 1 Score: 298.13 bits (762), Expect = 7.509e-96
Identity = 157/320 (49.06%), Postives = 207/320 (64.69%), Query Frame = 3 
Query:   75 DLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDV---QNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL 1025
            D  +TS GVGHH+RPGYYFPDSQF S +  PLP  LA P EI+    + TTTG  HD K+P G+     +  + PGH KV+Y+KD  E +   GWRRPL+   QK+ET D F   P + +  ++ +V   Q F L ++HN+GPSK  ++ST NPKL+G+  Y +D  VL  LDPY++T  K+HR F P EL    KKDI TYWDCEEYPK  GFG KEN +PK+++PR+  PMRDT +F +AT I R P +   VPHSG+++ Y D Y +PSDV+ K  H  P+D P  +PDP  NS    P+MY TEY + G+  P ++
Sbjct:   15 DWHQTSTGVGHHYRPGYYFPDSQFRSVMNEPLPRNLAAPDEIIDNQRYDTTTGKAHDNKHP-GTTYNNLVHLKPPGHWKVNYVKDLAEKLGQGGWRRPLTMGNQKTETQDNFRAQPGIKDHYRFEEVYHPQGFLLQDYHNEGPSK--VSSTTNPKLQGKPFYVRDGGVLPLLDPYLSTTHKHHRSFKPSELKKYPKKDIATYWDCEEYPKSMGFGMKENPLPKNSVPRDRLPMRDTMVFPTATKIPRQPKALVPVPHSGLKSVYKDNYTRPSDVRMKHNHYCPVDTPYTLPDPGTNSIMTAPKMYTTEYKNVGSGKPIVV 331
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Match: A0A2T7NAP2 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_21769 PE=4 SV=1)

HSP 1 Score: 293.508 bits (750), Expect = 4.972e-94
Identity = 151/321 (47.04%), Postives = 203/321 (63.24%), Query Frame = 3 
Query:   72 NDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDV---QNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL 1025
             D  +TS  VGHH+RPGYYFPD++F + +R+PLPP LAK  E+    H++TT+G +HD K P+  L    + K+APGH KV+Y+KD  E I   GWR+PL+   Q SE   E++  P L E   + +V   QNF L +HH DGPSK    ST+NPKL+G   Y +D+ +L NLDPY++T  K HR F   EL G AKKDIPT+WDCEEYPK  G G K N++PK  +PRE  PMRDT +F +AT I R P +   VPH G+++EY   Y+    V+ KE +   +D P  +P+P   S +  P+MY TEY   G++ P  +
Sbjct:   14 QDWHQTSTEVGHHYRPGYYFPDTEFTTIIRDPLPPYLAKEDELTRGQHYQTTSGFYHDRK-PHTLLYGERIHKKAPGHWKVNYIKDLAEKIGKGGWRQPLTMGNQLSEMKAEYNGQPGLREKYNFEEVFYPQNFTLQDHHKDGPSKYGEGSTQNPKLQGEPFYVRDKGILHNLDPYLSTTHKDHRSFKLSELKGYAKKDIPTFWDCEEYPKAWGHGQKHNSLPKHTVPREKLPMRDTMVFPTATKIPRPPKAMIPVPHQGLKSEYAGNYVPQDHVRQKENYYCAVDTPFVLPNPGTKSIFTAPKMYNTEYQHIGSNKPVTV 333
[Close]
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Nematostella
Match: EDO47628 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RLM0])

HSP 1 Score: 219.164 bits (557), Expect = 6.061e-69
Identity = 127/316 (40.19%), Postives = 181/316 (57.28%), Query Frame = 3 
Query:   72 NDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKW-LDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLR-NLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVP--DPNPNSSYEVPRMYETEYASYGN 1007
            NDL  TS G+GH  R G+ FP   F  T+    PP LA P  I  +DHFKTTTGT HD K P G L  +PL+ +APG+ KV Y++D +  +  + WRR L+   Q SE  D ++  P +    ++   +Q F+L +HH +G SK ++ ST N  +  +  YP+D+ VL  + DPY+TT  K HR F   E S   +KD  TYW+CE YPK  G G K N +P +++PR+  PMRD  +F+  TT+ R+P S + VP+ GM +   + Y  P + K  E     +  P   P   P P  +++VP++Y TEY  YG+
Sbjct:    7 NDLHLTSTGIGHQVRKGHVFPPEGFTRTIGKVYPPTLANPDWIHYEDHFKTTTGTTHDYKAP-GGLLSHPLYNKAPGNWKVKYVEDNISQLQVKPWRRALTMGNQCSEMKDNYTGRPGVNLTTQFNAGIQPFHLKDHHREGNSK-VVPSTRNQGIAAKKFYPRDRGVLTYHSDPYLTTTQKDHRAFTKLEQSRYPRKDYATYWECEGYPKAWGHGSKLNPLPPNSVPRQKGPMRDEMVFREGTTVPRLPKSLDPVPNKGMVSLMKETYTDPPERKRWELFTCDVPKPWHAPLEAPRPGETFKVPKVYSTEYQYYGS 320
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Planmine SMEST
Match: SMESG000061089.1 (SMESG000061089.1)

HSP 1 Score: 688.337 bits (1775), Expect = 0.000e+0
Identity = 333/333 (100.00%), Postives = 333/333 (100.00%), Query Frame = 3 
Query:   27 MTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL 1025
            MTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL
Sbjct:    1 MTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPPVLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLKDFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDVQNFNLFNHHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFDPKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTTIFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFPCAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL 333
The following BLAST results are available for this feature:
BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
AC008687.42.931e-2429.70novel protein[more]
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 3
Match NameE-valueIdentityDescription
AC151602.11.887e-927.55luteinizing hormone beta [Source:NCBI gene;Acc:168... [more]
AC151602.15.668e-927.55luteinizing hormone beta [Source:NCBI gene;Acc:168... [more]
AC151602.15.668e-927.55luteinizing hormone beta [Source:NCBI gene;Acc:168... [more]
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A267E3Z07.486e-11753.17Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A1I8JLA98.348e-11753.17Uncharacterized protein OS=Macrostomum lignano OX=... [more]
R7VGG94.399e-9648.05Uncharacterized protein OS=Capitella teleta OX=283... [more]
V4AK997.509e-9649.06Uncharacterized protein OS=Lottia gigantea OX=2251... [more]
A0A2T7NAP24.972e-9447.04Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO476286.061e-6940.19Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000061089.10.000e+0100.00SMESG000061089.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010966 ID=SMED30010966|Name=Core 1 udp-galactose:n-acetylgalactosamine-alpha-r beta 1,3-galactosyltransferase, putative|organism=Schmidtea mediterranea sexual|type=transcript|length=1102bp
CTTATGCTTAGCAACAATTCGGTCCTATGACTTCAATTACTGAAAGAAAT
ATTACCGTTAGACTTCCCACGAATGACTTATTTAAAACTAGTGTGGGTGT
CGGTCATCATTGGAGGCCGGGCTATTATTTTCCCGATTCTCAATTTAATT
CAACATTGAGAAACCCTCTTCCTCCAGTTCTTGCTAAACCACCTGAGATC
TTGCCACAAGATCATTTTAAAACAACAACTGGAACATTTCACGATGAGAA
ATATCCATATGGATCTTTACAACAATATCCTTTGTTTAAAAGAGCTCCAG
GTCATAATAAGGTACATTACCTAAAAGACTTCATGGAAAATATTCCTAAT
AGAGGATGGAGACGACCACTAAGTCCGCGTTATCAAAAGTCAGAAACACA
CGATGAATTCAGCAACCCACCAAAATTGTATGAACCTAATAAATGGTTAG
ATGTACAGAATTTCAATCTATTTAACCATCACAACGACGGTCCTTCAAAG
AAAATCATGGCGTCTACAGAAAATCCAAAGTTAAAAGGTAGAGTTCTTTA
TCCCAAGGATCAAGAAGTTCTTCGAAATTTAGATCCATATATGACCACAA
ACATGAAATATCACAGAGTTTTTGATCCAAAAGAACTTAGTGGTATAGCT
AAAAAAGATATACCAACTTATTGGGATTGTGAAGAATATCCGAAAGTTCG
AGGATTCGGTCCAAAAGAAAACACTGTGCCGAAATCCGCTATTCCCAGAG
AAAATCCACCGATGAGAGATACAACGATTTTCAAATCTGCAACTACTATT
CGACGAATTCCACCCTCTGGAGAAATTGTTCCTCACAGTGGGATGCAGAC
CGAATATGATGACAAATATATTAAACCATCGGACGTGAAAGCTAAAGAAG
CTCATTTAAGCCCAATAGATTTTCCATGTGCTGTTCCTGATCCAAATCCA
AACTCATCTTATGAAGTACCAAGAATGTATGAAACTGAATATGCAAGTTA
TGGCAATGATTATCCTACAATATTATAAAACAATATTTAAGAGTTCTATT
AGACACTCAGAAAATGTTTATTCATCAGATAATAAAGAGTATTCTATTGA
AA
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protein sequence of SMED30010966-orf-1

>SMED30010966-orf-1 ID=SMED30010966-orf-1|Name=SMED30010966-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=334bp
MTSITERNITVRLPTNDLFKTSVGVGHHWRPGYYFPDSQFNSTLRNPLPP
VLAKPPEILPQDHFKTTTGTFHDEKYPYGSLQQYPLFKRAPGHNKVHYLK
DFMENIPNRGWRRPLSPRYQKSETHDEFSNPPKLYEPNKWLDVQNFNLFN
HHNDGPSKKIMASTENPKLKGRVLYPKDQEVLRNLDPYMTTNMKYHRVFD
PKELSGIAKKDIPTYWDCEEYPKVRGFGPKENTVPKSAIPRENPPMRDTT
IFKSATTIRRIPPSGEIVPHSGMQTEYDDKYIKPSDVKAKEAHLSPIDFP
CAVPDPNPNSSYEVPRMYETEYASYGNDYPTIL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000020protonephridia
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000106ciliated epithelial cell
PLANA:0000418head
PLANA:0002032epidermal cell
Vocabulary: molecular function
TermDefinition
GO:0016263glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase activity
GO:0016740transferase activity
GO:0016757transferase activity, transferring glycosyl groups
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR37404FAMILY NOT NAMEDcoord: 16..331