SMED30010944

Overview
NameSMED30010944
Smed IDSMED30010944
Length (bp)3781
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010944 (SMED30010944) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010944 (SMED30010944) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30010944

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
central nervous systemSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
posterior region of the whole animalSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:27063937
Scimone et al., 2016
whole organism asexual adult colorimetric in situ hybridization evidence
pigment cellSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 SmedASXL_075274SmedAsxl_ww_GCZZ01PMID:29158443
He et al., 2017
whole organism asexual adult RNA-sequencing evidence
nervous systemSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30010944SMESG000062936.1 SMESG000024338.1 SMESG000024319.1 SMESG000024308.1 dd_Smed_v4_18000_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30010944SMESG000046431.1 SMESG000027653.1 SMESG000058066.1 SMESG000024319.1 OX_Smed_1.0.16323ox_Smed_v2PMID:24238224
Kao et al., 2013
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of SMED30010944 vs. Ensembl Zebrafish
Match: CABZ01033394.1 (pep chromosome:GRCz11:13:6032844:6038477:-1 gene:ENSDARG00000101795.2 transcript:ENSDART00000166130.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CABZ01033394.1)

HSP 1 Score: 109.383 bits (272), Expect = 1.918e-23
Identity = 110/452 (24.34%), Postives = 201/452 (44.47%), Query Frame = -3
Query:  597 ELDQITAKEVIFEYKQANASSPGDDNITYRDLRLL-DPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGL--EFRPEKCAYLSTSNSTD-INGXXXXXXXXX---XXKDKEFYHYLGVPVGECP-NQTPYDTLYPWQKVDMYK-TFLHSRLHFAFRTREIKISAI------GRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLT 1907
            EL+ +TA + +         +PG D +     ++  D  G+    + N  +  G +P   LS +  ++    K GD  +  +WRP++LL + +KI +  L+ RL   I    ++HP Q         +++ +++  +L+ S Y     L +  +D + AF  V H Y+WN L   G    F+ ++++LY    S       ++    I RG+RQGC +S +L++LAI P+L  + S  I  + + +  VQ    A         A D+   +N   ++ + + +  EF     A ++   S   ING   N   +     +  K    YLGV +GE    Q  +D +    +  + K  ++H +L  +FR R + I+ +       R    +  +  L      ++  F++  H  P++ +Y P+E GG   + L   ++   +    RLLT
Sbjct:  480 ELELLTALKAM-----QPGKAPGIDGLPIEFYKIFWDVLGEDFLAVLNESLTEGSLP---LSCRRAVVTLLPKKGDLQEIKNWRPVSLLCSDFKILSKTLANRLKNVI--GHIIHPDQTYCVPGRSIMDNISLVRDILDLSSYFN-LDLGLISIDQEKAFDRVEHQYLWNTLEAFGFGSGFIGMIKVLYQDIESVLKINGGLSAPFKINRGIRQGCALSGMLYSLAIEPMLRKLRSR-IEGFMLANKSVQHQLSAY--------ADDVIVFVNGQKDVNTLVSIISEFDKLSAARVNWGKSDALINGKWNNGTPILPGGLIWKKGGLKYLGVYLGESTMQQKNWDGIIEKIEGKLKKWRWIHPQL--SFRGRVLIINNLIASTLWHRLFCTEPPSGLLLFLQAKLVNFFWDRMHWVPQSELYLPLEEGGQGLVSLVSRHATFRLQFVQRLLT 909          
BLAST of SMED30010944 vs. Ensembl Zebrafish
Match: CU462878.3 (pep chromosome:GRCz11:17:8542203:8545189:1 gene:ENSDARG00000103562.2 transcript:ENSDART00000158873.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU462878.3)

HSP 1 Score: 107.457 bits (267), Expect = 5.441e-23
Identity = 71/238 (29.83%), Postives = 122/238 (51.26%), Query Frame = -3
Query: 1197 LDQITAKEVIFEYKQANAS-SPGDDNITYRDLRLLDPE-GKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1904
            L +IT +E+     +  A+ +PG D       +   P+   +L + FN ++  G+IP  W      +IPK GK  D ++ SS+RPI++L+  YK+F SILS+RL   ++ ++L+   Q G  +     ++      ++ H      + + ++ LD + AF SV   Y++ +LA  G +  F+  ++ LY    +       ++  I ++RG RQGCP+S  LF L I P+  AI   P
Sbjct:  264 LTEITIEELDSAISKLKANKTPGTDGYPSEMYKTFKPQITPMLLKCFNYVLRGGEIPQSWQEAVISIIPKQGK--DKNECSSYRPISVLNVDYKLFASILSKRLE--VVISELVDLDQTGFIQNRQTQDNLRRTLQIMSHITTENISAMLLS-LDAEKAFDSVGWDYLYQVLARFGFNNKFIECIKGLYLSPKAKIKINGHLSKTIYLERGTRQGCPLSPTLFALFIEPLAQAIREEP 496          
BLAST of SMED30010944 vs. Ensembl Zebrafish
Match: BX649434.3 (pep chromosome:GRCz11:3:18270043:18274691:-1 gene:ENSDARG00000105157.2 transcript:ENSDART00000161140.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX649434.3)

HSP 1 Score: 97.0561 bits (240), Expect = 1.168e-19
Identity = 65/220 (29.55%), Postives = 112/220 (50.91%), Query Frame = -3
Query: 1200 NASSPGDDNITYRDLRLLDPE-GKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSS 1856
            N  SPG D I     ++L P  G+ L  + N  ++ G +P   LS +  +I    K GD     +WRP++LL + YKI +  L+ RL+K  + ++++ P Q          ++  ++   LE +K        I+ LD + AF  V H Y+  +L   G   HF+ ++ ++Y+   S       ++   D++RGVRQGC +S +L+++AI P+L  I + 
Sbjct:  496 NGKSPGIDGIPVDFYKVLWPVIGEDLFLVLNDSLNKGSLP---LSCRRAVITLLPKKGDLQKIGNWRPVSLLCSDYKILSRALAVRLSK--VLDQIIQPNQNYSIPNRSIFDNIFLIRDALEVAKLFGINCGLIS-LDQEKAFDRVEHEYLKKVLKVFGFSTHFIKMIEVMYSNIQSVLKINGGLSAPFDVQRGVRQGCAMSGMLYSIAIEPLLHRIRAE 709          
BLAST of SMED30010944 vs. Ensembl Zebrafish
Match: BX323564.1 (pep chromosome:GRCz11:2:10597810:10600950:-1 gene:ENSDARG00000098557.2 transcript:ENSDART00000160216.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:BX323564.1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.505e-18
Identity = 103/413 (24.94%), Postives = 175/413 (42.37%), Query Frame = -3
Query:  642 SPGDDNITYR-DLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPV-LVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALIN--NSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDTLYPWQK--VDMYKTFLHSRLHFAFRTREIKISAIGR---PSAKDSHNKNLSTQLRSIIYSFF--NLPHNAPKAYIYSPIETGGAAFIDLHD 1847
            SPG D ++    L   +     L  ++   I+  ++P         L+PKP K  D     +WRPI LL+  YK+ + I ++RL + +  N+++   Q G         +  ++  +L++S   +   L + +LD   AF ++ H +++  L Y G  + F+S++ L +   NS     P  + +  I RGVRQGCPI+  LF L +  + +  + SS I    + + E++I   ADD  L       L A +   N   IAS  GL     KC  +S  NS            L  +  K+   YLG+ + +  ++       P  K    ++  +L   L    R    K+  + R   P+        +  ++  +   F   N  H+  K  I  P + GG   +D  D
Sbjct:  187 SPGSDGLSVEFYLHFWNIIENPLFEMYKESIELKELPTSLKQGLITLLPKPNK--DLMILDNWRPITLLNVDYKLLSLIYAKRLKEGL--NEIISEYQTGFMAGRHISWNIRLILDLLDYSNLIESEALLL-FLDFHKAFDTIEHQFMFMALKYFGFGDRFISIMELFHRDINSSINLYPNTSKRFPICRGVRQGCPIAPFLFLLVVEFLSIYVLKSSAIQGITIFNKEIRISQLADDTVLFLKDKDQLPAALQLVNDFSIAS--GLTLNVTKCEIISLYNSE--------VKSLFNIPVKKTVKYLGISITKNLSERQDLNFSPKIKKAQGIFNNWLQRDLSIIGRVLLTKVEGVSRFVYPALSLFVQDAICKKINDLFIQFVWRNRHHHLRKEVIQGPRKDGGFELLDFFD 584          
BLAST of SMED30010944 vs. Ensembl Zebrafish
Match: CU638714.1 (pep chromosome:GRCz11:24:19863246:19868789:1 gene:ENSDARG00000100303.2 transcript:ENSDART00000165242.2 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:CU638714.1)

HSP 1 Score: 92.8189 bits (229), Expect = 1.977e-18
Identity = 74/250 (29.60%), Postives = 123/250 (49.20%), Query Frame = -3
Query: 1209 KSKEIIELDQITAKEVIFEYKQ-ANASSPGDDNITYRDLRLLDPE-GKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLT---KWIIENKLLHPGQKGGSRFEGCVEHNAV------LTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAI 1925
            + K++++ D IT KE+    +Q +N  SPG D +     R    E G  L  +F        +P   LS +  ++    K GD     +WRP++LL   YKI +  L+ R+    +++IEN   +           CV   ++      L  V++ S  + K  L +  LD + AF  V H Y++N+L   G  E F+S ++LLY+        G  ++ ++ I RG+RQGCP+S  L++L I P+L+ +
Sbjct:  468 EQKKLLDRD-ITFKELTEAMQQLSNGRSPGIDGLPVEFYRQFWNEIGMDLYEVFLSCFKEKLLP---LSCRRAVLSLLPKKGDLCLLKNWRPVSLLCLDYKILSKCLANRIMHCLQFLIENDQTY-----------CVPDRSIIDNIFMLRDVVDFSHLTSK-DLGVLSLDQEKAFDRVDHGYLFNVLKNFGFGEEFLSYIKLLYSNVFIMVKAGGALSAQVPIGRGIRQGCPLSGQLYSLMIEPLLLKL 701          
BLAST of SMED30010944 vs. Ensembl Xenopus
Match: ENSXETT00000032317.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:130520927:130524992:-1 gene:ENSXETG00000008594.1 transcript:ENSXETT00000032317.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 117.087 bits (292), Expect = 9.723e-26
Identity = 80/241 (33.20%), Postives = 121/241 (50.21%), Query Frame = -3
Query: 1197 ELDQITAKEVIFEYKQANASSPGDDN----ITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1907
            EL  +  +E +  +    A+  G D     I  R  +LL P   +L +LF   +  GQ P        +L+PK GK  D     S+RPI+LL+   KIF  +L+RRL++ I   K++HP Q G    +    +   L   L H+   + A  AIA LDI  AF +V   Y+W +L + G  + ++ +++LLY    +      L TP   + RG RQGCP+S +LF +AI P   A+ + P
Sbjct:  448 ELTAVEIQEAVNAFPLGKAA--GADGLPIEIYKRHSKLLTP---MLLKLFKEAVQLGQFPESLYEAAIVLLPKQGK--DPHLCESFRPISLLTADVKIFAKVLARRLSRVI--TKIIHPDQIGFIPAKTAALNTRRLYLNLAHASAGQGAK-AIAALDITKAFDTVEWPYLWQVLTHFGFGKKYIQMVQLLYKYPVASIRINSLTTPAFALSRGTRQGCPLSPLLFAIAIEPFAQAVRAHP 678          
BLAST of SMED30010944 vs. Ensembl Xenopus
Match: ENSXETT00000040456.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:8:87387561:87390644:-1 gene:ENSXETG00000021299.1 transcript:ENSXETT00000040456.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 116.701 bits (291), Expect = 1.018e-25
Identity = 79/241 (32.78%), Postives = 121/241 (50.21%), Query Frame = -3
Query: 1197 ELDQITAKEVIFEYKQANASSPGDDN----ITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1907
            EL  +  +E +  +    A+  G D     I  R  +LL P   +L +LF   +  GQ P        +L+PK GK  D     S+RPI+LL+   KIF  +L+RRL++  +  K++HP Q G    +    +   L   L H+   + A  AIA LDI  AF +V   Y+W +L + G  + ++ +++LLY    +      L TP   + RG RQGCP+S +LF +AI P   A+ + P
Sbjct:   78 ELTAVEIQEAVNAFPLGKAA--GADGLPIEIYKRHSKLLTP---MLLKLFKEAVQLGQFPESLYEAAIVLLPKQGK--DPHLCESFRPISLLTADVKIFAKVLARRLSR--VITKIIHPDQIGFIPAKTAALNTRRLYLNLAHASAGQGAK-AIAALDITKAFDTVEWPYLWQVLTHFGFGKKYIQMVQLLYKYPVASIRINSLTTPAFALSRGTRQGCPLSPLLFAIAIEPFAQAVRAHP 308          
BLAST of SMED30010944 vs. Ensembl Xenopus
Match: ENSXETT00000002428.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:3:15907829:15913358:1 gene:ENSXETG00000006437.1 transcript:ENSXETT00000002428.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.383 bits (272), Expect = 2.683e-23
Identity = 82/250 (32.80%), Postives = 122/250 (48.80%), Query Frame = -3
Query: 1194 KEIIE--LDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGK----LLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKG--GSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPI 1919
            +E +E  +  I  +E I  + Q  A+  G D I    + L     K    LL +++   I +G +P        IL+PKPGK  D     S+RPI+LL+T  KI+  IL+RRL + I    L++P Q G   ++          L   L  S    +A   IA LDI  AF +V  +Y+W +L  +G    F+++++LLY    +      L T    + RG RQGCP+S +LF LAI P   A+   P+
Sbjct:  442 REFLEAKITDIETQEAIDSFPQGKAA--GSDGI---PIELYKKHAKSQTPLLTKMYGEAISTGILPESMYEAAIILLPKPGK--DPQLCESFRPISLLTTDVKIYAKILARRLARVI--KTLVNPDQIGFIPTKTTALNTRRLYLNLSLTPSNTGNRA---IAALDIAKAFDTVEWSYLWCVLKQLGFGPTFINMVQLLYKSPRATLRINSLCTSNFTLSRGTRQGCPLSPLLFALAIEPFAQAVRKHPL 679          
BLAST of SMED30010944 vs. Ensembl Xenopus
Match: ENSXETT00000045854.1 (pep primary_assembly:Xenopus_tropicalis_v9.1:1:73083463:73084749:1 gene:ENSXETG00000020482.1 transcript:ENSXETT00000045854.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 101.293 bits (251), Expect = 6.959e-22
Identity = 80/261 (30.65%), Postives = 125/261 (47.89%), Query Frame = -3
Query: 1197 EFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANAS--SPGDDNIT---YRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKG--GSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1958
            +++ SI    I   +   LD    K+ + E    + S  +PG D +    Y+ L    P  KLL  L     D+  +P  +     ++IPKPGK  D    SS+RPI+L++T  KI   IL+ RL + + +  L+HP Q G    R              + H +   +    +A LD   AF S+   Y+W +L+  G  + F+  ++LLY    +      + +P   ++RG RQGCP+S ILF LAI P+ + I +SP
Sbjct:  132 QYLASIPIPQIAPPDKAYLDAPITKQEVAEAIATHPSNKTPGPDGLPSNWYKVLAEYIP-SKLLETL-QYAYDNQALPASFAEALIVVIPKPGK--DPSLCSSYRPISLINTDAKILAKILATRLQRVVPD--LVHPDQSGFMPGRSTDINLRRLFTNLQIPHLETDTRV---VASLDSAKAFDSIEWGYLWEVLSGFGFGQTFLKWIKLLYQHPTARVRVNGITSPPFSLERGTRQGCPLSPILFALAIEPLAILIRNSP 383          
BLAST of SMED30010944 vs. Ensembl Xenopus
Match: efcab14 (EF-hand calcium binding domain 14 [Source:Xenbase;Acc:XB-GENE-5791088])

HSP 1 Score: 99.3673 bits (246), Expect = 4.198e-21
Identity = 72/222 (32.43%), Postives = 110/222 (49.55%), Query Frame = -3
Query: 1197 SPGDDNIT---YRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKG--GSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1847
            +PG D +    Y+ L    P  KLL  L     D+  +P  +     ++IPKPGK  D    SS+RPI+L++T  KI   IL+ RL + + +  L+HP Q G    R              + H +   +    +A LD   AF S+   Y+W +L+  G  + F+  ++LLY    +      + +P   ++RG RQGCP+S ILF LAI P+ + I +SP
Sbjct:  121 TPGPDGLPSNWYKVLAEYIP-SKLLETL-QYAYDNQALPASFAEALIVVIPKPGK--DPSLCSSYRPISLINTDAKILAKILATRLQRVVPD--LVHPDQSGFMPGRSTDINLRRLFTNLQIPHLETDTRV---VASLDSAKAFDSIEWGYLWEVLSGFGFGQTFLKWIKLLYQHPTARVRVNGITSPPFSLERGTRQGCPLSPILFALAIEPLAILIRNSP 333          
BLAST of SMED30010944 vs. UniProt/SwissProt
Match: sp|Q03274|PO22_POPJA (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 1.208e-22
Identity = 70/245 (28.57%), Postives = 126/245 (51.43%), Query Frame = -3
Query: 1164 ITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGP-LITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEV 1895
            I  +E+    K    S+PG D +T + +       +L      + +  G +P  W + +T LIPK    GD ++ S+WRPI + S   ++   IL++RL   +     LHP QKG +R +G + ++ +L   +   +  +K    ++ LD++ AF +V H+ I   L  +G+DE   + +    + + +    GP   T KI I+RGV+QG P+S  LFN  ++ +L ++ S+P     +G+ ++
Sbjct:    6 IAREEIQCAIKGWKPSAPGSDGLTVQAI----TRTRLPRNFVQLHLLRGHVPTPWTAMRTTLIPK---DGDLENPSNWRPITIASALQRLLHRILAKRLEAAV----ELHPAQKGYARIDGTLVNSLLLDTYISSRREQRKTYNVVS-LDVRKAFDTVSHSSICRALQRLGIDEGTSNYITGSLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQSTPGIGGTIGEEKI 238          
BLAST of SMED30010944 vs. UniProt/SwissProt
Match: sp|Q03278|PO21_NASVI (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Nasonia vitripennis OX=7425 PE=4 SV=2)

HSP 1 Score: 108.612 bits (270), Expect = 1.840e-22
Identity = 123/443 (27.77%), Postives = 201/443 (45.37%), Query Frame = -3
Query:  666 EIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPK-PGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAY----IGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEF--------YHYLGV------PV-------GECPNQTPYDTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGG 1916
            EI  L +  + + I E +    ++ G D +T      +D   + +  LFN+I+  GQ P  +L  +T+LIPK PG      D + +RP+++ S + + F  IL+ R    I E+ LL   Q+     +G  E+ ++L+A+++ ++   K  L IA LD+K AF SV H  I + L      + +  + + V R   T+       G  I P     RGVRQG P+S +LFN  ++ VL  +  +    + MG  ++  L +ADD+ L+A +   LQA ++         GLE  P KC  L+   S     + +   K   + ++E         + YLGV      P+       G+    T    L P Q++ +   FL  R         I    +GR S  D    +    +R  +  +  LPH+ P  Y ++PI+ GG
Sbjct:  311 EIKNLWRPISNDEIKEVEACKRTAAGPDGMTTTAWNSID---ECIKSLFNMIMYHGQCPRRYLDSRTVLIPKEPGTM----DPACFRPLSIASVALRHFHRILANR----IGEHGLLDTRQRAFIVADGVAENTSLLSAMIKEARMKIKG-LYIAILDVKKAFDSVEHRSILDALRRKKLPLEMRNYIMWVYRNSKTRLEVVKTKGRWIRP----ARGVRQGDPLSPLLFNCVMDAVLRRLPEN--TGFLMGAEKIGALVFADDLVLLAETREGLQASLSRIEAGLQEQGLEMMPRKCHTLALVPSGKEKKIKVETHKPFTVGNQEITQLGHADQWKYLGVVYNSYGPIQVKINIAGDLQRVTAA-PLKPQQRMAILGMFLIPRF--------IHKLVLGRTSNADVRKGD--KIIRKTVRGWLRLPHDTPIGYFHAPIKEGG 724          
BLAST of SMED30010944 vs. UniProt/SwissProt
Match: sp|P16423|POLR_DROME (Retrovirus-related Pol polyprotein from type-2 retrotransposable element R2DM OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 6.980e-21
Identity = 114/454 (25.11%), Postives = 208/454 (45.81%), Query Frame = -3
Query:  648 SKEIIELDQ--------ITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNST-------DINGXXXXXXXXXXXKDKEFYHYLGV--------------PVGECPNQTPYDTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDL 1922
            S E+I++D         IT ++ +   + + +SSPG D IT +  R + P G +L R+ N+I+  G +P+     +T+ IPK   +    D   +RPI++ S   +   +IL+ RL   I  +    P Q+G    +GC ++  ++  VL HS    ++   IA LD+  AF S+ HA I++ L   G  + FV  ++  Y    +        + +    RGV+QG P+S ILFNL ++ +L  + S   AK  +G++     A+ADD+ L A +   LQ L++ +++  S +GL+   +KC  +             +     + + ++  LK  + + YLG+               +G    +     L P Q++   +T L  +L+       + I  +          +     +R  +  + NLP + P A++++P ++GG     L
Sbjct:  327 SGEVIQMDHSLERVWSAITEQD-LRASRVSLSSSPGPDGITPKSAREV-PSGIML-RIMNLILWCGNLPHSIRLARTVFIPKTVTAKRPQD---FRPISVPSVLVRQLNAILATRLNSSINWD----PRQRGFLPTDGCADNATIVDLVLRHSHKHFRS-CYIANLDVSKAFDSLSHASIYDTLRAYGAPKGFVDYVQNTYEGGGTSLNGDGWSSEEFVPARGVKQGDPLSPILFNLVMDRLLRTLPSEIGAK--VGNAITNAAAFADDLVLFAETRMGLQVLLDKTLDFLSIVGLKLNADKCFTVGIKGQPKQKCTVLEAQSFYVGSSEIPSLKRTDEWKYLGINFTATGRVRCNPAEDIGPKLQRLTKAPLKPQQRLFALRTVLIPQLYHKLALGSVAIGVL----------RKTDKLIRYYVRRWLNLPLDVPIAFVHAPPKSGGLGIPSL 757          
BLAST of SMED30010944 vs. UniProt/SwissProt
Match: sp|O00370|LORF2_HUMAN (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 90.5077 bits (223), Expect = 6.927e-17
Identity = 67/238 (28.15%), Postives = 108/238 (45.38%), Query Frame = -3
Query: 1161 SPGDDNITYRDLRLLDPE-GKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLH-------PGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAI-SSSPIAKYRMGDSEVQ 1847
            SPG D  T    +    E    L +LF  I   G +PN +     ILIPKPG+  D     ++RPI+L++   KI   IL+ R+ + I   KL+H       PG +G       +        V++H   +K     I  +D + AF  +   ++   L  +G+D  ++ ++R +Y K  +             +K G RQGCP+S +LFN+ +  +  AI     I   ++G  EV+
Sbjct:  470 SPGPDGFTAEFYQRYKEELVPFLLKLFQSIEKEGILPNSFYEASIILIPKPGR--DTTKKENFRPISLMNIDAKILNKILANRIQQHI--KKLIHHDQVGFIPGMQGWFNIRKSIN-------VIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLARAIRQEKEIKGIQLGKEEVK 696          
BLAST of SMED30010944 vs. UniProt/SwissProt
Match: sp|P08548|LIN1_NYCCO (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 3.951e-16
Identity = 65/238 (27.31%), Postives = 109/238 (45.80%), Query Frame = -3
Query: 1161 SPGDDNITYRDLRLLDPE-GKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLH-------PGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAI-SSSPIAKYRMGDSEVQ 1847
            SPG D  T    +    E   +L  LF  I   G +PN +      LIPKPGK  D     ++RPI+L++   KI   IL+ R+ + I   K++H       PG +G       +        V++H    K     I  +D + AF ++ H ++   L  IG++  F+ ++  +Y+K  +      +      ++ G RQGCP+S +LFN+ +  + +AI     I    +G  E++
Sbjct:  469 SPGPDGFTSEFYQTFKEELVPILLNLFQNIEKEGILPNTFYEANITLIPKPGK--DPTRKENYRPISLMNIDAKILNKILTNRIQQHI--KKIIHHDQVGFIPGSQGWFNIRKSIN-------VIQHINKLKNKDHMILSIDAEKAFDNIQHPFMIRTLKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEVLAIAIREEKAIKGIHIGSEEIK 695          
BLAST of SMED30010944 vs. TrEMBL
Match: A0A159X4Q6 (ORF2-like protein (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 639.802 bits (1649), Expect = 0.000e+0
Identity = 314/319 (98.43%), Postives = 314/319 (98.43%), Query Frame = -3
Query: 1455 ICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIA 2411
            I SYI   LTDRS FKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIA
Sbjct:    1 IXSYIXXXLTDRSXFKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIA 319          
BLAST of SMED30010944 vs. TrEMBL
Match: A0A3L8D8E5 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010823 PE=4 SV=1)

HSP 1 Score: 246.128 bits (627), Expect = 2.219e-62
Identity = 198/637 (31.08%), Postives = 287/637 (45.05%), Query Frame = -3
Query:   78 LDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEI-ASRIGLEFRPEKCAYLSTSNST---------DINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDT---------------LYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWING--KSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVS-WNFVHRARTNNLAINASKLDG**DE 1904
            +   T  EV    ++   S+PG D I Y DLR  DP  +LL  LFN       +P  W +  T+L+    K GD D   SWRP+AL  T  K+F ++ + RLT W+I N  L   QKG  R EGC EHN VL  +L  ++ +++  + +AWLD+ +AFGSVPHA I + L   GV    +++   +Y    +        T  I I+ GVRQGCP+S I+F+L I+ V+ A +      Y +      ILAYADD+ALIA +   ++ L+  +VE+ A R+GL F P KCA L  +            +I G       +  L + E Y +LGVP G   +QTP  T               L PWQ+V++   F+  RL F  R   ++   +    A D H       +R I+ S+ NLP  A    +Y P   GG   + L D   +LTI  AFRLLT    + S +   SLR V + RI    PS  +   +++G  +   +       W      A              E  G   +           +    R ++   L   V E Y   L        + +   + P+ N  +        + W FVHRAR + L +N ++  G  DE
Sbjct:  643 MAPFTEGEVDRRLRRMTNSAPGPDGIAYNDLRAADPGARLLTALFNACYRLEAVPPSWKTSNTVLV---YKKGDRDSLESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTRRHAV-VAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINIWGSMYDGCTTRVRAVDGFTAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELADVGYDILGQTFNILAYADDLALIARTPEGMRQLL-TAVELEAGRVGLHFNPAKCASLHITTGRIGRVLPTVFEIEG-----RPMPTLAEGEPYRHLGVPTGFSVDQTPLTTIEGVLKDIWAVDASLLAPWQRVEVLAAFILPRLDFLLRGAAVEKRPL---RAVDLH-------VRRIVKSWLNLPQRASAEVVYLPPSRGGCGLLPLSDLADVLTIAHAFRLLTASDAVVSGLAWGSLRGVVARRIG-HAPSDEEIASFLSGSLEGRLRGGGEASLWSRARNAARRQANRAAVRWRWSEVTGEMIVECRGPGERIVRVPGSARAQVIHRLRSAVMEYYAGTLLRKPDQGKVFEVSSRVPVSNHFMRGGSFTRFADWRFVHRARLDVLPLNGARRWGTVDE 1258          
BLAST of SMED30010944 vs. TrEMBL
Match: A0A3L8DAI7 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010948 PE=4 SV=1)

HSP 1 Score: 244.202 bits (622), Expect = 1.051e-61
Identity = 195/637 (30.61%), Postives = 287/637 (45.05%), Query Frame = -3
Query:   78 LDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEI-ASRIGLEFRPEKCAYLSTSNST---------DINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDT---------------LYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWING--KSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVS-WNFVHRARTNNLAINASKLDG**DE 1904
            +   T  EV    ++   S+PG D I Y DLR  DP  +LL  LFN       +P  W +  T+L+    K GD D   SWRP+AL  T  K+F ++ + RLT W+I N  L   QKG  R EGC EHN VL  +L  ++ +++  + +AWLD+ +AFGSVPHA I + L   GV    +++   +Y    +        T  I I+ GVRQGCP+S I+F+L I+ V+ A +      Y +      ILAYADD+ALIA +   ++ L+  +VE+ A R+GL F P KCA L  +            +I G       +  L + E Y +LGVP G   +QTP  T               L PWQ+V++   F+  RL F  R   ++   +          + +   +R I+ S+ NLP  A    +Y P   GG   + L D   +LTI  AFRLLT    + S +   SLR V + RI  + PS  +   +++G  +   +       W      A              E  G   +           +    R ++   L   V E Y   L        + +   + P+ N  +        + W FVHRAR + L +N ++  G  DE
Sbjct:  868 MAPFTEGEVDRRLRRMTNSAPGPDGIAYNDLRAADPGARLLTALFNACYRLEAVPPSWKTSNTVLV---YKKGDRDSLESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTRRHAV-VAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINIWGSMYDGCTTRVRAVDGFTAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELTDVGYDILGQTFNILAYADDLALIARTPEGMRQLL-TAVELEAGRVGLHFNPAKCASLHITTGRIGRVLPTVFEIEG-----RPMPTLAEGEPYRHLGVPTGFSVDQTPLTTIEGVLKDIWAVDASLLAPWQRVEVLAAFILPRLDFLLRGAAVEKRPL----------RAVDLHVRRIVKSWLNLPQRASAEVVYLPPSRGGCGLLPLSDLADVLTIAHAFRLLTASDAVVSGLAWGSLRGVVARRIGHA-PSDEEIASFLSGSLEGRLRGGGEASLWSRARNAARRQANRAAVRWRWSEVTGEMIVECRGPGERIVRVPGSARAQVIHRLRSAVMEYYAGTLLRKPDQGKVFEVSSRVPVSNHFMRGGSFTRFADWRFVHRARLDVLPLNGARRWGTVDE 1483          
BLAST of SMED30010944 vs. TrEMBL
Match: K7JWY9 (Reverse transcriptase domain-containing protein OS=Nasonia vitripennis OX=7425 PE=4 SV=1)

HSP 1 Score: 241.506 bits (615), Expect = 1.217e-61
Identity = 218/766 (28.46%), Postives = 333/766 (43.47%), Query Frame = -3
Query:   99 NERRKICNILLAEIATCNANEAYSK-IISAANKSNIKPEHKPYL--GKNFFNSLNSD---------NKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMD---------PDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTD----INGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDTLY---------------PWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSII----YSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASF-LHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNT-VSWNFVHRARTNNLAINASK 2258
             E+R+I  +L A        EA ++ ++  A  +  +PE +  +  G    +    D          +  K++  Y  ++K+   +IL   N++ +     I   F ++          PD  VF   +    S E +    IT +EV    K  N +SPG D ITYRDL   DP   +LA L+N I      P  W    T LI    K GD  D S+WRPI+L  T  K+F +IL+ R+ +W + N      QKG   FEGC EHN VL   +  +K  +K  L +AWLD+  AF SVPH+ I   L   G+     +++  LYT   + +      T  I I+ GVRQGCP+S  +FNL +  VL  I  +    Y +       LAYADD+A++A+S   ++ L+  +   A  +GL F P KCA L  +   +        ++    ++ L  +E Y +LG+P G    QTP +TL                PWQK+D   TFL  RL F  +   I              +K   T+   II     S+ +LP  A    ++ P   GG   + L D Y +LT+  ++++L       S +   +LR   S R++    +I     +++G      S S  T+W + R A L +K   G+      +G   L    + R      S   K L +  L + +   Y   L   K    + +   +    N  +          W F+HRAR + L +NA+K
Sbjct:  265 TEQRRIVGLLEAATGREQLEEATTQAMLFLARLTGRRPEPRNAIRPGPRQRHPAQEDVQAQAPDRIQEAKKLQRLYRTSKKRAVQKILAGPNMNCQIDKNTITAHFVELAARRDGGEDWPD--VFDREEPTAASGEAL-CTPITREEVFRRLKGRNNTSPGPDGITYRDLAKADPGAHVLAALYNSIWRIEATPALWGVSNTTLI---YKKGDAMDISNWRPISLGDTVPKLFAAILADRIKRWAVANGRYSASQKGFLEFEGCYEHNFVLQEAIREAKGGRKE-LVVAWLDLASAFTSVPHSSILQALEGHGLPSKARNIISSLYTGMTTRFHTAEGPTDPILIQSGVRQGCPLSPDVFNLTLEVVLREIQRTGEG-YTIEGRRTSHLAYADDVAILADSPAGMRRLLFAAERGARAVGLTFNPAKCATLHIAGRGEEAVRPTEFSVQGTPVRALASEEAYEHLGIPTGYQVRQTPINTLRDLLADIGSIDRSLLAPWQKLDAVGTFLLPRLDFIMQGAHI--------------DKGFLTEADKIIKKAAKSWLSLPQRASAELVFLPPSQGGGGLLPLADLYDMLTVAHSYKMLYSSDVTVSTIAGSTLRRTVSERLKKRASNID-IARFLSGDLDLPRSTSPSTFWTKVRSAALRIKTKLGLRWSWCQEGEVLLMACGDPRAPGTRVSPQTKHLVTTSLRRCLNRHYAESLLAKKDQGKVFEVTRRSGQSNHFLRSGSFTRFCDWRFIHRARLDVLPLNAAK 1007          
BLAST of SMED30010944 vs. TrEMBL
Match: A0A3L8D7Z7 (Reverse transcriptase domain-containing protein OS=Ooceraea biroi OX=2015173 GN=DMN91_010459 PE=4 SV=1)

HSP 1 Score: 243.817 bits (621), Expect = 1.471e-61
Identity = 198/637 (31.08%), Postives = 288/637 (45.21%), Query Frame = -3
Query:   78 LDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEI-ASRIGLEFRPEKCAYLSTSNST---------DINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDT---------------LYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWING--KSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVS-WNFVHRARTNNLAINASKLDG**DE 1904
            +   T  EV    ++   S+PG D I Y DLR  DP  +LL  LFN       +P  W +  T+L+    K GD D   SWRP+AL  T  K+F ++ + RLT W+I N  L   QKG  R EGC EHN VL  +L  ++ +++  + +AWLD+ +AFGSVPHA I + L   GV    +++   +Y    +        T  I I+ GVRQGCP+S I+F+L I+ V+ A +      Y +      ILAYADD+ALIA +   ++ L+  +VE+ A R+GL F P KCA L  +            +I G       +  L + E Y +LGVP G   +QTP  T               L PWQ+V++   F+  RL F  R   ++   +    A D H       +R I+ S+ NLP  A    +Y P   GG   + L D   +LTI  AFRLLT    + S +   SLR V + RI  + PS  +   +++G  +   +       W      A              E  G   +           +    R ++   L   V E Y   L        + +   + P+ N  +        + W FVHRAR + L +N ++  G  DE
Sbjct:  944 MAPFTEGEVDRRLRRMTNSAPGPDGIAYNDLRAADPGARLLTALFNACYRLEAVPPSWKTSNTVLV---YKKGDRDSLESWRPLALGDTMPKLFAAVAADRLTDWVIANDKLCRAQKGFLRDEGCYEHNFVLQEILTDARRTRRHAV-VAWLDLSNAFGSVPHAAIRSALVRAGVPSGLINIWGSMYDGCTTRVRAVDGFTAPIPIRSGVRQGCPLSPIIFDLVIDSVVRAAAELTDVGYDILGQTFNILAYADDLALIARTPEGMRQLL-TAVELEAGRVGLHFNPAKCASLHITTGRIGRVLPTVFEIEG-----RPMPTLAEGEPYRHLGVPTGFSVDQTPLTTIEGVLKDIWAVDASLLAPWQRVEVLAAFILPRLDFLLRGAAVEKRPL---RAVDLH-------VRRIVKSWLNLPQRASAEVVYLPPSRGGCGLLPLSDLADVLTIAHAFRLLTASDAVVSGLAWGSLRGVVARRIGHA-PSDEEIASFLSGSLEGRLRGGGEASLWSRARNAARRQANRAAVRWRWSEVTGEMIVECRGPGERIVRVPGSARAQVIHRLRSAVMEYYAGTLLRKPDQGKVFEVSSRVPVSNHFMRGGSFTRFADWRFVHRARLDVLPLNGARRWGTVDE 1559          
BLAST of SMED30010944 vs. Ensembl Cavefish
Match: ENSAMXT00000034560.1 (pep primary_assembly:Astyanax_mexicanus-2.0:APWO02000712.1:195631:202616:-1 gene:ENSAMXG00000042811.1 transcript:ENSAMXT00000034560.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 135.191 bits (339), Expect = 1.652e-31
Identity = 122/451 (27.05%), Postives = 215/451 (47.67%), Query Frame = -3
Query:  603 TAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKC-AYLSTSNST-----DINGXXXXXXXXXXXKDKEFYHYLGVPV---------------GECPNQTPYDTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRL 1892
            T KE + + K+  A  PG D IT + L   DP G+ L RL+   + +G +P  +   +T LIPK       ++  +WRPI + S   ++F+ I++ RL +       ++P Q+G     GC E+  VL  ++ H K  +++ LA+ ++D   AF S+ H +I + L    +D+H + +++  Y    +        +P I +K GV+QG  +S ILFNLA++P++ ++ +   + Y++G S V  LA+ADD+ L+++S   +++ I    E   R GL  +  KC  +L    +T     D +  +L    +  ++  E   YLGV V               GE   +     L P+QKV +   F   RL +     ++K   +            L   +R  +  + +LP +     +YS ++ GG   + L    +++  +QA RL
Sbjct:  430 TVKENLGQIKRNTA--PGPDRITRQMLSDWDPSGEKLERLYTAWMVAGVVPKAFKECRTTLIPKSTSKEALEEVGNWRPITIGSLILRLFSKIMAERLARACP----INPRQRGFISAPGCAENLKVLQGLIGHCK-KERSQLAVVFVDFARAFDSISHEHILSALGQRQLDQHVIKLIQSAYVDCVTRIIMDGAKSPDILMKVGVKQGDSMSPILFNLAMDPLIQSLENLG-SGYKIGGSSVTTLAFADDLVLLSSSWDGMRSNIALLDEFCERTGLRVQSRKCHGFLIRRGATSYTVNDCSPWDLGGEPIHLIEPHEVEKYLGVKVNPWVGITKPDLNSQLGEWVTRIGGAPLKPYQKVGLLNDFAIPRLIYLADHCDLKGVTLA----------TLDGTIRRAVKGWLHLPLSTCGGLLYSRVQDGGLGILKLE---ALVPSIQARRL 859          
BLAST of SMED30010944 vs. Ensembl Cavefish
Match: ENSAMXT00000034831.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:16211297:16215948:1 gene:ENSAMXG00000034228.1 transcript:ENSAMXT00000034831.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 118.242 bits (295), Expect = 2.608e-26
Identity = 71/230 (30.87%), Postives = 120/230 (52.17%), Query Frame = -3
Query: 1170 ASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPG-QKGG-SRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDS 1853
             S+PG + + YR  +      K L +L  I+   G IP +W     +LIPK   S   D    +RPI+LL+   KIF S+++RRL  ++  N L+    QK G + F GC+EHN+++   ++ ++  KK  L + +LD+ +AFGSVPHA +W    +  V E    +++  +      +  G  +T    ++ G+  GC IS++ F +A+  V++  S   +   R+ D 
Sbjct:  387 GSAPGPNGVPYRSYKSAPDVLKFLWKLMAIVWKKGIIPKEWHRAGGVLIPKEKDSVAIDQ---FRPISLLNVEGKIFFSVVARRLATYLKRNNLIDTSVQKAGIAGFSGCIEHNSMIWHQIQTARAEKK-DLHVVFLDLANAFGSVPHALLWKAFNFFRVPEEISRLVKAYFEDIQFCFSVGECVTSWQRLEIGIMAGCTISLLAFTMAME-VIIRASKWVVGGERLHDG 611          
BLAST of SMED30010944 vs. Ensembl Cavefish
Match: ENSAMXT00000046127.1 (pep primary_assembly:Astyanax_mexicanus-2.0:7:16384999:16389616:1 gene:ENSAMXG00000035718.1 transcript:ENSAMXT00000046127.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.9821 bits (245), Expect = 7.433e-21
Identity = 54/166 (32.53%), Postives = 92/166 (55.42%), Query Frame = -3
Query: 1197 KSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSP 1694
            K GD  + ++WR + L++  YKIF  +L  RL K  I +K+++  Q  G +     ++  ++  ++ + K+  ++   I  LD K AF S+   Y+W +L      + F+ +++LLY K+         +T KIDI RGV+QGCP+S  L+ LAINP+L ++    
Sbjct:  144 KKGDEYELNNWRQVTLMNIEYKIFAKVLMNRLEK--ILDKIINEEQTCGIKGRSMWDNLCIIRELITN-KHLNESGFYILALDKKKAFDSISREYLWAVLERYNFPDVFIKMIKLLYLKSTVQVKVNGKLTEKIDICRGVKQGCPLSAALYVLAINPLLTSLKKEE 306          
BLAST of SMED30010944 vs. Ensembl Cavefish
Match: ENSAMXT00000033573.1 (pep primary_assembly:Astyanax_mexicanus-2.0:3:31407009:31410218:-1 gene:ENSAMXG00000030324.1 transcript:ENSAMXT00000033573.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.7525 bits (247), Expect = 1.192e-20
Identity = 71/223 (31.84%), Postives = 115/223 (51.57%), Query Frame = -3
Query: 1227 KEVIFEYKQANASS-PGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPG-QKGG-SRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAIN 1886
            KEV    K+A +SS PG   + Y+  +      + L ++  +I   G++   W   + + IPK   S + D    +R I+LLS   KIF SIL+RRLT ++  N  +    QKGG     GC+EH  V+T ++  ++ +K   LA+ WLD+ +A+GS+PH  +   L    V      ++   Y + +     G + +    ++ G+  GC IS+ILF LA+N
Sbjct:  260 KEVQDVVKKARSSSAPGPSGVPYKVYKNCPLLLERLWKILKVIWRRGKVARQWRFAEGVWIPKEEVSKNIDQ---FRIISLLSVEGKIFFSILARRLTDFLSSNHYIDTSVQKGGIPGVPGCLEHTGVVTQLIREARENK-GDLAVLWLDLANAYGSIPHKLVQTTLERHHVPGQVAELIMNYYDQFSMRVSTGSITSEWHRVEVGIITGCTISVILFVLAMN 478          
BLAST of SMED30010944 vs. Ensembl Cavefish
Match: ENSAMXT00000048276.1 (pep primary_assembly:Astyanax_mexicanus-2.0:17:7857318:7859867:1 gene:ENSAMXG00000040121.1 transcript:ENSAMXT00000048276.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 98.9821 bits (245), Expect = 1.806e-20
Identity = 78/264 (29.55%), Postives = 130/264 (49.24%), Query Frame = -3
Query: 1188 DEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANAS-----SPGDDNITYRDL-RLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAK 1961
            D F+  ++   +   ++++LD     E   E K+A  S     SPG D I    L  + D  G +  +     ++ G    D  S    L+ KPGK  D  + SS+RP++LL+T  K+F  +L++RL   I +  L+H  Q G  R     ++   L  +LE +  S+    A+  LD + AF  +   ++W +L   G    F+ ++  LYT  ++    G +I+ K  ++RG RQGCP+S  LF LA+ P+  A+  + + K
Sbjct:  431 DNFLKPLNLSSLAPHQVMQLDAPITLE---ELKEAIISMQTHKSPGLDGIPPELLVAVWDLVGPIFLKSVEYSLEIGSFHRDQNSALISLLLKPGK--DSTECSSYRPLSLLNTDVKVFAKVLAKRLDSVIPD--LIHSDQTGFIRSRLASDNLRRLFHILEFAP-SQMLGSAVLSLDAEKAFDRLEWPFLWAVLKRFGFGSAFIHMIFTLYTNPSACVSTGHMISAKFLLERGTRQGCPLSPFLFALAVEPLAQAVRQNALIK 686          
BLAST of SMED30010944 vs. Ensembl Nematostella
Match: EDO47846 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RKZ5])

HSP 1 Score: 53.5286 bits (127), Expect = 1.551e-8
Identity = 37/109 (33.94%), Postives = 56/109 (51.38%), Query Frame = -3
Query: 1551 KQANASSPGDDNITYRDLRL----LDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKG 1865
            K  N  +PG D I    L+     L P    ++++FN+I+D+G  P+ W      +I    KSGD  D S +R I + S   K+F  IL+ RL  ++  N++LH  Q G
Sbjct:    2 KLKNNKAPGIDRIRNEMLKCGQQTLVP---CISKIFNLILDAGVYPDVWTRG---IISAIYKSGDKSDPSDYRGICVTSCLGKLFCFILNNRLQTFLTSNQILHQSQIG 104          
BLAST of SMED30010944 vs. Ensembl Nematostella
Match: EDO35961 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SJY4])

HSP 1 Score: 47.7506 bits (112), Expect = 1.062e-6
Identity = 28/85 (32.94%), Postives = 46/85 (54.12%), Query Frame = -3
Query: 1551 LDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKG 1805
            ++ +G+   +LFN ++  G     W       I +  KSGD  D  ++R IA+ S   K FT I++ RLTK++ + K+L+  Q G
Sbjct:   17 VESQGRYFEKLFNTVLAQGVFSKSW---SKGYIVQLHKSGDKCDRGNYRGIAISSCLGKFFTLIINGRLTKFLKKYKILNRYQIG 98          
BLAST of SMED30010944 vs. Ensembl Medaka
Match: ENSORLT00000043450.1 (pep primary_assembly:ASM223467v1:12:7314868:7318593:-1 gene:ENSORLG00000025257.1 transcript:ENSORLT00000043450.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.916 bits (263), Expect = 1.503e-22
Identity = 93/309 (30.10%), Postives = 145/309 (46.93%), Query Frame = -3
Query:  999 EIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLL-DPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNS--TDING 1916
            E+I L+++T      +Y + N  SPG D +T    +   D     L ++F   I+ G +P         LIPKP K  D     +WRPI LL+  YKI   IL+ R+    + + ++   Q G  +      +  ++  VL++S+  +   L I +LD   AF S+ H +I   L   G  + F + ++ LY  +NS        +P+  +KRGVRQGCP+S+ LF L++  + + I  SP+    + + E+ I   ADD AL    A+ +   IN     +   GL     KC  L   N   T I G
Sbjct:  447 EVISLNEVTDA---IKYLKIN-KSPGTDGLTAEFYKQFSDNLAPFLLKVFEESINKGALPPTLSQGLITLIPKPRK--DPLSLDNWRPITLLNCDYKILALILANRIKP--VLDTIIDENQSGFMQNRHISNNIRLILDVLDYSELVQDDSL-ILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKNCLLTSIQG 746          
BLAST of SMED30010944 vs. Ensembl Medaka
Match: ENSORLT00000028663.1 (pep primary_assembly:ASM223467v1:7:26712747:26716472:-1 gene:ENSORLG00000028539.1 transcript:ENSORLT00000028663.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 105.916 bits (263), Expect = 1.568e-22
Identity = 119/439 (27.11%), Postives = 191/439 (43.51%), Query Frame = -3
Query:  648 EIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLL-DPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNS--TDINGXXXXXXXXXXXKDKEFYHYLGVPV-----GECP-NQTPY-----DTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFF--NLPHNAPKAYIYSPIETGGAAFIDL 1916
            ++I L+++T      +Y + N  SPG D +T    +   D     L ++F   I+ G +P         LIPKP K  D     +WRPI LL+  YKI   IL+ R+    + + ++   Q G  +      +  ++  +L++S+  +   L I +LD   AF S+ H +I   L   G  + F + ++ LY  +NS        +P+  +KRGVRQGCP+S+ LF L++  + + I  SP+    + + E+ I   ADD AL    A+ +   IN     +   GL     KC  L   N   T I G+ +          K    YLG+ +       C  N +P       TL  W + D+    L  R+     T+   IS +   +     N  +   +  I+Y+F   N  H   K+ I +  + GG   ID 
Sbjct:  447 DVISLNEVTDA---IKYLKIN-KSPGKDGLTAEFYKQFSDNLAPFLLKVFEESINKGALPPTLSQGLITLIPKPRK--DPLSLDNWRPITLLNCDYKILALILANRIKP--VLDTIIDENQSGFMQNRHISNNIRLILDILDYSELVQDDSL-ILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKNCLLTSIQGIPV----------KTSVTYLGIQITKDMQSRCSTNFSPIIDKTKKTLNQWLQRDLS---LKGRV---LLTKAEGISRLTYAAQSLQVNNTVCNTINRILYNFLWRNKTHYIRKSVILNTSDKGGLNCIDF 860          
BLAST of SMED30010944 vs. Ensembl Medaka
Match: ENSORLT00000035976.1 (pep primary_assembly:ASM223467v1:16:7718738:7727892:1 gene:ENSORLG00000029559.1 transcript:ENSORLT00000035976.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 102.449 bits (254), Expect = 1.633e-22
Identity = 72/224 (32.14%), Postives = 108/224 (48.21%), Query Frame = -3
Query: 1203 KQANASSPGDDNIT---YRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISS 1865
            +Q    SPG D +T   Y+  R  D    LL+R+F      G++P   L    +L        D D+   +RPI LL+T YKI TS+L+ RL + +    +L   Q  G +     +    L   +EH + S    L +   D   AF  V H ++W +L   G  E F+S +R LYT  +S   C    T    I R +RQGCP+S +LF++A+ P+   I++
Sbjct:  104 RQNRNKSPGADGLTAEFYQCFR--DDLVPLLSRMFGSFC-LGRLPTFCLGTVKLLFKNKRDRADLDN---YRPITLLNTDYKILTSVLAERLREVL--PSILGSSQAYGVQGRQITDTVLSLLCTVEHMERSGFFFLNV---DFSKAFDRVEHGFLWRVLDLFGFGELFISRIRALYTTASSRVLCNGHSTGVFSITRSIRQGCPLSAMLFSIAVEPLAGVITA 316          
BLAST of SMED30010944 vs. Ensembl Medaka
Match: ENSORLT00000037544.1 (pep primary_assembly:ASM223467v1:22:28708275:28712000:-1 gene:ENSORLG00000028184.1 transcript:ENSORLT00000037544.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 104.375 bits (259), Expect = 4.270e-22
Identity = 118/439 (26.88%), Postives = 191/439 (43.51%), Query Frame = -3
Query:  648 EIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLL-DPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNS--TDINGXXXXXXXXXXXKDKEFYHYLGVPV-----GECP-NQTPY-----DTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFF--NLPHNAPKAYIYSPIETGGAAFIDL 1916
            ++I L+++T      +Y + N  SPG D +T    +   D     L ++F   I+ G +P         LIPKP K  D     +WRPI LL+  YKI   IL+ R+    + + ++   Q G  +      +  ++  +L++S+  +   L I +LD   AF S+ H +I   L   G  + F + ++ LY  +NS        +P+  +KRGVRQGCP+S+ LF L++  + + I  SP+    + + E+ I   ADD AL    A+ +   IN     +   GL     KC  L   N   T I G+ +          K    YLG+ +      +C  N +P        L  W + D+    L  R+     T+   IS +   +     N  +   +  I+Y+F   N  H   K+ I +  + GG   ID 
Sbjct:  447 DVISLNEVTDA---IKYLKIN-KSPGKDGLTAEFYKQFSDNLAPFLLKVFEESINKGALPPTLSQGLITLIPKPRK--DPLSLDNWRPITLLNCDYKILALILANRIKP--VLDTIIDENQSGFMQNRHISNNIRLILDILDYSELVQDDSL-ILFLDFYKAFDSLEHNFIIAALNKFGFGQFFCNAVQTLYVNSNSSIKLASGTSPRFFLKRGVRQGCPLSVYLFLLSVQLLNLHIKLSPLKGLIVAEREIFISQLADDTALFLRDASQVAVAINIIQSFSKASGLTLNLNKCEILPVKNCLLTSIQGIPV----------KTSVTYLGIQITKDMQSKCSTNFSPIIDKTKKNLNQWLQRDLS---LKGRV---LLTKAEGISRLTYAAQSLQVNNTVCNTINRILYNFLWRNKTHYIRKSVILNTSDKGGLNCIDF 860          
BLAST of SMED30010944 vs. Ensembl Medaka
Match: ENSORLT00000045921.1 (pep primary_assembly:ASM223467v1:18:29376025:29377426:1 gene:ENSORLG00000028454.1 transcript:ENSORLT00000045921.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 99.3673 bits (246), Expect = 4.482e-22
Identity = 70/210 (33.33%), Postives = 105/210 (50.00%), Query Frame = -3
Query: 1194 RLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRF----EGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPI 1811
            ++L P   +L   FN    +G +P        IL+PKPGK  +     + RPI+LL++  KI   IL+ RL     +N L H   +  + F    +G   HN      + H      A  A+   D + AF  V   +++N+LA +G  E F   ++LLYT   +       I+  I+IKRG RQGCP+S +LF LAI P+ +A+ + PI
Sbjct:   90 KILKPMLDMLQESFN----NGALPKSMTCALIILLPKPGKPSN--KCENMRPISLLNSDLKIICKILAMRL-----QNTLPHVVHRDQNGFILGRQGL--HNVRRVLNIIHG-LEDTADQALLSFDAEKAFDKVEWPFLFNVLAKLGFGETFRKWIQLLYTNPTAEILTNKNISAPIEIKRGCRQGCPLSPLLFTLAIEPLAIAVRAHPI 285          
BLAST of SMED30010944 vs. Planmine SMEST
Match: SMESG000062936.1 (SMESG000062936.1)

HSP 1 Score: 1763.04 bits (4565), Expect = 0.000e+0
Identity = 917/1011 (90.70%), Postives = 927/1011 (91.69%), Query Frame = -3
Query:  105 LLSNLKIIGETLTMLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKASFKXXXXXXXXXXXSDDWIDCEVGRYIPNSLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDT---------------LYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLAINA 3092
            LLSNLKIIGETLTMLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCK+SFKIIINSINNNNISDDWIDCE GRYI NS+K+NKI LTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLL+IDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLT VGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISA NKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYC KSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKS                    + T++L                                      EHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLL YIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQILAYADDIALIANSATDLQALI +SVEIASRIGLEFRPEKCAYLSTSNS+DINGVNLN+VKLKKLKDKEFYHYLGVPVGECPNQTPYDT               LYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCP TSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTN LAINA
Sbjct:  371 LLSNLKIIGETLTMLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKSSFKIIINSINNNNISDDWIDCEFGRYIDNSIKANKIPLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLIIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTCVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAVNKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCTKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKS--------------------VLTAVL--------------------------------------EHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLTYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQILAYADDIALIANSATDLQALIESSVEIASRIGLEFRPEKCAYLSTSNSSDINGVNLNDVKLKKLKDKEFYHYLGVPVGECPNQTPYDTLEKLLSDANKIKNSDLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPNTSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNTLAINA 1323          

HSP 2 Score: 92.0485 bits (227), Expect = 2.313e-18
Identity = 46/51 (90.20%), Postives = 48/51 (94.12%), Query Frame = -1
Query: 3074 DEVASDNLIVINETFIIDNITYKQFKEIAYIVCPFRSNDVIYIFFFYLI*K 3226
            DEVASDNLIVINETFIIDNITYKQFKEIAYIVCPFRSNDVI+IFF+  I K
Sbjct:  309 DEVASDNLIVINETFIIDNITYKQFKEIAYIVCPFRSNDVIHIFFYNKISK 359          
BLAST of SMED30010944 vs. Planmine SMEST
Match: SMESG000024308.1 (SMESG000024308.1)

HSP 1 Score: 882.863 bits (2280), Expect = 0.000e+0
Identity = 446/456 (97.81%), Postives = 451/456 (98.90%), Query Frame = -3
Query: 1686 MLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKASFKXXXXXXXXXXXSDDWIDCEVGRYIPNSLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSG 3053
            MLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKASFKIIINSINNNNISDDWIDCE GRYIPNS+K+NKI LTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLL+IDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLT VGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISA NKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFN+IIDSG+IPNDWLSFKTILIPKPGK G
Sbjct:    1 MLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKASFKIIINSINNNNISDDWIDCEFGRYIPNSIKANKIPLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLIIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTCVGNKLESILLPPKQNTDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAVNKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNLIIDSGEIPNDWLSFKTILIPKPGKVG 456          
BLAST of SMED30010944 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 691.419 bits (1783), Expect = 0.000e+0
Identity = 410/944 (43.43%), Postives = 564/944 (59.75%), Query Frame = -3
Query:  105 SLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLP----PKQNTDQNILKLPLICKT---INERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSIS-DYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDI-LLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLAINA 2864
            S+K  + +   LN+  IN K++     KYF +L  N  + L+NET+  A++ D  +H+ +FL+   L+ A +IF I  PP   + LL+IDY     +++NPT+            I  N I+ ++   G    M    +DIR  GL S +LIC++I NY  D  L  ++L  +G  + S LLP     K+    ++ K+  I K+   + +R+   + LL +++    NE    II       +K +HKPYLG          NK I ++  +  + K    ++ +  N+  RP+ + I+  F    P +  ++   ++C +SK+ + L+  +  E++F+ K+A+ +SPG D I YRDL LLDPEGKLL  LFN II +  IP+ W SFKT+L PKP K G YDD SSWRPIALLS  YK+F S L+ RLT WI  N LLH GQKGGSR +GCVEHN++L+A LEHSKYSK  PLAIAWLDIKDAFGSVPH Y+W+LL +IGV E F S+L+LLY+ T+++Y CGP++TP I IK+GV+QGCPISMILF LAINPVL A+S S    + +G+S V+ILAYADDIALIA S  DLQ + N +V  A+ IG E+RPEKC YL        + + +NN K+KKL  KEFY YLGVPVGE P+Q+PYD                L  WQK+  YK F+HSRL F FRTREIK  A+ + +  ++ + N+S+QLR        LP+ +  +Y Y+  E GGA  +DL DEY   TI   FRLLT +C     +  DSL+FV   R+ I  PS+ + L+W NGK  K +   +KT + R R A+   + T  I +  +  D   SL IT   RGT I+ S  RK  +  LH  + ++Y        ++NFIA      P INKAIF+S L+  +WNF+HRARTN L+I A
Sbjct: 2730 SVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPTTLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISS-LLPIISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIID-IIVRQFPDRVKSKHKPYLGNK--TRPQKINKGILVRK-FMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFQGNWNNPLNFCKRSKQALNLELFSNFEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKIIVNKIIPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFTSILQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKIKKLLSKEFYQYLGVPVGEEPDQSPYDILDKAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNNNRSVNISSQLRGCFRKMLCLPNQSEVSYFYNATEKGGANCVDLLDEYHTQTITHFFRLLTSECDYARQINIDSLKFVTGPRLGIKTPSLQESLDWFNGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFA 3668          

HSP 2 Score: 690.263 bits (1780), Expect = 0.000e+0
Identity = 410/944 (43.43%), Postives = 563/944 (59.64%), Query Frame = -3
Query:  105 SLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLP----PKQNTDQNILKLPLICKT---INERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSIS-DYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDI-LLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLAINA 2864
            S+K  + +   LN+  IN K++     KYF +L  N  + L+NET+  A++ D  +H+ +FL+   L+ A +IF I  PP   + LL+IDY     +++NPT+            I  N I+ ++   G    M    +DIR  GL S +LIC++I NY  D  L  ++L  +G  + S LLP     K+    ++ K+  I K+   + +R+   + LL +++    NE    II       +K +HKPYLG          NK I ++  +  + K    ++ +  N+  RP+ + I+  F    P    ++   ++C +SK+ + L+  +  E++F+ K+A+ +SPG D I YRDL LLDPEGKLL  LFN II +  IP+ W SFKT+L PKP K G YDD SSWRPIALLS  YK+F S L+ RLT WI  N LLH GQKGGSR +GCVEHN++L+A LEHSKYSK  PLAIAWLDIKDAFGSVPH Y+W+LL +IGV E F S+L+LLY+ T+++Y CGP++TP I IK+GV+QGCPISMILF LAINPVL A+S S    + +G+S V+ILAYADDIALIA S  DLQ + N +V  A+ IG E+RPEKC YL        + + +NN K+KKL  KEFY YLGVPVGE P+Q+PYD                L  WQK+  YK F+HSRL F FRTREIK  A+ + +  ++ + N+S+QLR        LP+ +  +Y Y+  E GGA  +DL DEY   TI   FRLLT +C     +  DSL+FV   R+ I  PS+ + L+W NGK  K +   +KT + R R A+   + T  I +  +  D   SL IT   RGT I+ S  RK  +  LH  + ++Y        ++NFIA      P INKAIF+S L+  +WNF+HRARTN L+I A
Sbjct: 5086 SVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPTTLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISS-LLPIISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIID-IIVRQFPDRVKSKHKPYLGNK--TRPQKINKGILVRK-FMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFHGNWNNPLNFCKRSKQALNLELFSNFEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKIIVNKIIPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFTSILQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKIKKLLSKEFYQYLGVPVGEEPDQSPYDILDKAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNNNRSVNISSQLRGCFRKMLCLPNQSEVSYFYNATEKGGANCVDLLDEYHTQTITHFFRLLTSECDYARQINIDSLKFVTGPRLGIKTPSLQESLDWFNGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFA 6024          

HSP 3 Score: 334.724 bits (857), Expect = 6.507e-94
Identity = 202/445 (45.39%), Postives = 267/445 (60.00%), Query Frame = -3
Query:  114 LLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLE-YDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLA 1400
            LL ++ V   FV++L+LLYT T+S+Y C  + TP I IK+GV+QGCPISMILF +AINPVL AIS S    + +G S VQ+LAYADDIALIA +++DLQ L + +V  A+ IG E+RPEKCAY+       ++ + +NNV +KKL   EFY YLGVP+G+  +Q+PY+                LY WQK+  YK FLHSRL F FRTREIK S++  PS     N N ++QLR  +    NLP+++  +Y Y+  E GGA  IDL DEY   TIV  FRLLT  C  +  +   SL  V S R+ I  P++SQ  EWINGK  K N   +KT + R R +I +      I +L E +    S+RIT    GT II    RK L+  LH  +Q++Y         +N +      CPL+NK IF+S L    W F+HRARTN L+
Sbjct:  902 LLKHMNVSAEFVNILQLLYTDTSSFYQCNQMQTPDIPIKKGVKQGCPISMILFAIAINPVLEAISLSNTTPFMIGTSSVQVLAYADDIALIATNSSDLQTLFDLAVNTANMIGFEYRPEKCAYIQYPKVETVSEIIVNNVLIKKLASNEFYQYLGVPIGDNNDQSPYEIVSKVIEDTKKIAHSNLYGWQKLKAYKIFLHSRLIFPFRTREIKTSSLADPSRNSRINVNNTSQLRGHLRKILNLPNHSEISYFYNSTENGGAGCIDLLDEYHAQTIVYFFRLLTSTCGYSKTINTFSLLSVTSPRLGIKNPTLSQSFEWINGKESKLNHSGRKTRFHRLRTSIEYFSRVHNIKLLFEIFKQNPSIRITTSLTGTRIILPKLRKTLSKILHSALQDSYLSKWEMSNHSNLLVSAIKLCPLVNKLIFRSDLTDFEWKFIHRARTNTLS 1346          

HSP 4 Score: 122.094 bits (305), Expect = 2.274e-27
Identity = 119/445 (26.74%), Postives = 211/445 (47.42%), Query Frame = -3
Query: 1875 CLYSLSL*IQ*CNIYIF----LLSNLKIIGETLTMLFNQFCRSEIVQMSTDY--SKAQFMEYDDLNVCKASFKXXXXXXXXXXXSDDWIDCEVGRYIPNSLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQ------------NILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANK-SNIKPEH--KPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMD-PDEFVFSISDYCIKSKEIIELDQITAKEVI 3143
            CL+  S+ ++  +I+ F    +   L  IG  +T   NQFC +  V+   ++  S + F+ YD        F      +    + +  +DC+      + +K+      +  +  IN K+    I +YF  L  +D Y L+N+TL  A+  D  Q++ +FL+   ++ A++IF I  PP   + LLVIDY     + L+P ++    +     + +L  I+ +R + G A       HDIR  GL S ILIC++I NY  D SL  ++L  +G K+ + L+P    +D             N LKL L      +R++  N LL +++  + +E    I++   +  N++ +   +PYLGKN     ++  K       +  N K    RIL++ ++   P  + I+N FT  D   E   ++  +   S E++EL  I+++E++
Sbjct:  467 CLFIYSIKLKHSHIFDFNYRTIDDRLIEIGVKITDYLNQFCLNRNVKFLANHHISHSTFLSYD-----TQYFASHFIVLFEKLVYEIHLDCDKVYDSLSKIKTETCIEISPGLNLINGKITDKIIFEYFNKLKLSDDYILLNKTLCVAISDDCTQYMKDFLNIECMMKANVIFAIISPPKVHELLLVIDYSTEEHYYLSPVTTDLNVVSQHFCYNILIKINELRESKGRAMKTGHCRHDIRGSGLLSKILICAFIRNYALDYSLENINLHEIG-KIVNSLVPIVDGSDNVKYEKREKLKAANTLKLNL-----KQRKEKINQLLIKLSDSDVDEIICTILTNIPRLENLQKKEFREPYLGKN-----HNKPKKFNNPAEFLTNMKTTVYRILNDQSVKIEPPFQEIINNFTHPDVVPECWDNVLKFSKISDELLELTYISSEEIL 895          

HSP 5 Score: 71.2478 bits (173), Expect = 5.832e-12
Identity = 42/142 (29.58%), Postives = 72/142 (50.70%), Query Frame = -1
Query: 3317 DNITFSDGLFYATNKVTEFDFST--GCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPQEIRK----ETHHRRCTIRL 3724
            + I+F  G F   N+  + D S   G SDI+FN+G +NF+++   +   + +      +EKII++IF LN L+L+ ++   S   Y  +  NY+ NC  L+S+  ++      L+D + W P  ++       H   C  RL
Sbjct: 2010 EQISFVGGKFIKLNETKDSDHSQAEGESDIMFNIGLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERL 2151          

HSP 6 Score: 71.2478 bits (173), Expect = 5.832e-12
Identity = 42/142 (29.58%), Postives = 72/142 (50.70%), Query Frame = -1
Query: 3317 DNITFSDGLFYATNKVTEFDFST--GCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPQEIRK----ETHHRRCTIRL 3724
            + I+F  G F   N+  + D S   G SDI+FN+G +NF+++   +   + +      +EKII++IF LN L+L+ ++   S   Y  +  NY+ NC  L+S+  ++      L+D + W P  ++       H   C  RL
Sbjct: 4359 EQISFVGGKFIKLNETKDSDHSQAEGESDIMFNIGLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERL 4500          
BLAST of SMED30010944 vs. Planmine SMEST
Match: SMESG000021486.1 (SMESG000021486.1)

HSP 1 Score: 690.649 bits (1781), Expect = 0.000e+0
Identity = 410/944 (43.43%), Postives = 564/944 (59.75%), Query Frame = -3
Query:  105 SLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLP----PKQNTDQNILKLPLICKT---INERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSIS-DYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDI-LLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLAINA 2864
            S+K  + +   LN+  IN K++     KYF +L  N  + L+NET+  A++ D  +H+ +FL+   L+ A +IF I  PP   + LL+IDY     +++NPT+            I  N I+ ++   G    M    +DIR  GL S +LIC++I NY  D  L  ++L  +G  + S LLP     K+    ++ K+  I K+   + +R+   + LL +++    NE    II       +K +HKPYLG          NK I ++  +  + K    ++ +  N+  RP+ + I+  F    P +  ++   ++C +SK+ + L+  +  E++F+ K+A+ +SPG D I YRDL LLDPEGKLL  LFN II +  IP+ W SFKT+L PKP K G YDD SSWRPIALLS  YK+F S L+ RLT WI  N LLH GQKGGSR +GCVEHN++L+A LEHSKYSK  PLAIAWLDIKDAFGSVPH Y+W+LL +IGV E F S+L+LLY+ T+++Y CGP++TP I IK+GV+QGCPISMILF LAINPVL A+S S    + +G+S V+ILAYADDIALIA S  DLQ + N +V  A+ IG E+RPEKC YL        + + +NN K+KKL  KEFY YLGVPVGE P+Q+PYD                L  WQK+  YK F+HSRL F FRTREIK  A+ + +  ++ + N+S+QLR        LP+ +  +Y Y+  E GGA  +DL DEY   TI   FRLLT +C     +  DSL+FV   R+ I  PS+ + L+W NGK  K +   +KT + R R A+   + T  I +  +  D   SL IT   RGT I+ S  RK  +  LH  + ++Y        ++NFIA      P INKAIF+S L+  +WNF+HRARTN L+I A
Sbjct: 2701 SVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPTTLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISS-LLPIISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIID-IIVRQFPDRVKSKHKPYLGNK--TRPQKINKGILVRK-FMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFQGNWNNPLNFCKRSKQALNLELFSNFEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKIIVNKIIPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFTSILQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKIKKLLSKEFYQYLGVPVGEEPDQSPYDILDKAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNNNRSVNISSQLRGCFRKMLCLPNQSEVSYFYNATEKGGANCVDLLDEYHTQTITHFFRLLTSECDYARQINIDSLKFVTGPRLGIKTPSLQESLDWFNGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFA 3639          

HSP 2 Score: 689.493 bits (1778), Expect = 0.000e+0
Identity = 410/944 (43.43%), Postives = 563/944 (59.64%), Query Frame = -3
Query:  105 SLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLP----PKQNTDQNILKLPLICKT---INERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVFSIS-DYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDI-LLEYDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLAINA 2864
            S+K  + +   LN+  IN K++     KYF +L  N  + L+NET+  A++ D  +H+ +FL+   L+ A +IF I  PP   + LL+IDY     +++NPT+            I  N I+ ++   G    M    +DIR  GL S +LIC++I NY  D  L  ++L  +G  + S LLP     K+    ++ K+  I K+   + +R+   + LL +++    NE    II       +K +HKPYLG          NK I ++  +  + K    ++ +  N+  RP+ + I+  F    P    ++   ++C +SK+ + L+  +  E++F+ K+A+ +SPG D I YRDL LLDPEGKLL  LFN II +  IP+ W SFKT+L PKP K G YDD SSWRPIALLS  YK+F S L+ RLT WI  N LLH GQKGGSR +GCVEHN++L+A LEHSKYSK  PLAIAWLDIKDAFGSVPH Y+W+LL +IGV E F S+L+LLY+ T+++Y CGP++TP I IK+GV+QGCPISMILF LAINPVL A+S S    + +G+S V+ILAYADDIALIA S  DLQ + N +V  A+ IG E+RPEKC YL        + + +NN K+KKL  KEFY YLGVPVGE P+Q+PYD                L  WQK+  YK F+HSRL F FRTREIK  A+ + +  ++ + N+S+QLR        LP+ +  +Y Y+  E GGA  +DL DEY   TI   FRLLT +C     +  DSL+FV   R+ I  PS+ + L+W NGK  K +   +KT + R R A+   + T  I +  +  D   SL IT   RGT I+ S  RK  +  LH  + ++Y        ++NFIA      P INKAIF+S L+  +WNF+HRARTN L+I A
Sbjct: 5028 SVKIKQANEICLNLNSINCKINEEITAKYFSNLKLNSNFMLLNETMCTAILDDCTRHLTKFLNYEDLIKAQVIFAIIAPPKHREILLIIDYITDEYYIINPTTLCGTESSQSVCRIFRNKINEIKETPGRVINMGDIPNDIRGSGLVSKLLICAFIKNYANDLPLINLNLREIGTNISS-LLPIISESKKILMSDLTKITKIKKSNLNLEQRKLKIDELLHDVSHLTVNEIID-IIVRQFPDRVKSKHKPYLGNK--TRPQKINKGILVRK-FMTDMKWTVDKVFNNTNVSIRPTFQKIVLNFLIKSPFHGNWNNPLNFCKRSKQALNLELFSNFEIMFQLKRADNTSPGIDGIQYRDLLLLDPEGKLLTFLFNKIIVNKIIPDSWKSFKTLLTPKPNKDGKYDDVSSWRPIALLSVIYKVFASCLASRLTYWINTNNLLHIGQKGGSRHDGCVEHNSILSAALEHSKYSKNCPLAIAWLDIKDAFGSVPHDYMWSLLRFIGVGEDFTSILQLLYSDTSTFYSCGPILTPNIPIKQGVKQGCPISMILFALAINPVLEAVSRSDCEPFMIGESPVKILAYADDIALIAKSVEDLQKITNIAVRTAAEIGFEYRPEKCGYLQLPKVHIDSEILINNTKIKKLLSKEFYQYLGVPVGEEPDQSPYDILDKAVSDTRKLANSELLGWQKLKAYKIFVHSRLIFPFRTREIKTGALSKSNGNNNRSVNISSQLRGCFRKMLCLPNQSEVSYFYNATEKGGANCVDLLDEYHTQTITHFFRLLTSECDYARQINIDSLKFVTGPRLGIKTPSLQESLDWFNGKESKPHHSGRKTRFQRARIAVAFFEKTHNISVSFIVKDHKPSLYITTAMRGTIILTSDLRKTTSKVLHMALCDSYLSKWEKSCVSNFIASAVKLSPKINKAIFRSELSEFAWNFIHRARTNTLSIFA 5966          

HSP 3 Score: 334.339 bits (856), Expect = 8.829e-94
Identity = 202/445 (45.39%), Postives = 267/445 (60.00%), Query Frame = -3
Query:  114 LLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYD---------------TLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLE-YDGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVKCPLINKAIFKSRLNTVSWNFVHRARTNNLA 1400
            LL ++ V   FV++L+LLYT T+S+Y C  + TP I IK+GV+QGCPISMILF +AINPVL AIS S    + +G S VQ+LAYADDIALIA +++DLQ L + +V  A+ IG E+RPEKCAY+       ++ + +NNV +KKL   EFY YLGVP+G+  +Q+PY+                LY WQK+  YK FLHSRL F FRTREIK S++  PS     N N ++QLR  +    NLP+++  +Y Y+  E GGA  IDL DEY   TIV  FRLLT  C  +  +   SL  V S R+ I  P++SQ  EWINGK  K N   +KT + R R +I +      I +L E +    S+RIT    GT II    RK L+  LH  +Q++Y         +N +      CPL+NK IF+S L    W F+HRARTN L+
Sbjct:  902 LLKHMNVSAEFVNILQLLYTDTSSFYQCNQMQTPDIPIKKGVKQGCPISMILFAIAINPVLEAISLSNTTPFMIGTSSVQVLAYADDIALIATNSSDLQTLFDLAVNTANMIGFEYRPEKCAYIQYPKVETVSEIIVNNVLIKKLASNEFYQYLGVPIGDNNDQSPYEIVSKVIEDTKKIAHSNLYGWQKLKAYKIFLHSRLIFPFRTREIKTSSLADPSRNSRINVNNTSQLRGHLRKILNLPNHSEISYFYNSTENGGAGCIDLLDEYHAQTIVYFFRLLTSTCGYSKTINTFSLLSVTSPRLGIKNPTLSQSFEWINGKESKLNHSGRKTRFHRLRTSIEYFSRVHNIKLLFEIFKQNPSIRITTSLTGTRIILPKLRKTLSKILHSALQDSYLSKWEMSNHSNLLVSAIKLCPLVNKLIFRSDLTDFEWKFIHRARTNTLS 1346          

HSP 4 Score: 121.709 bits (304), Expect = 2.514e-27
Identity = 119/445 (26.74%), Postives = 211/445 (47.42%), Query Frame = -3
Query: 1875 CLYSLSL*IQ*CNIYIF----LLSNLKIIGETLTMLFNQFCRSEIVQMSTDY--SKAQFMEYDDLNVCKASFKXXXXXXXXXXXSDDWIDCEVGRYIPNSLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQ------------NILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANK-SNIKPEH--KPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMD-PDEFVFSISDYCIKSKEIIELDQITAKEVI 3143
            CL+  S+ ++  +I+ F    +   L  IG  +T   NQFC +  V+   ++  S + F+ YD        F      +    + +  +DC+      + +K+      +  +  IN K+    I +YF  L  +D Y L+N+TL  A+  D  Q++ +FL+   ++ A++IF I  PP   + LLVIDY     + L+P ++    +     + +L  I+ +R + G A       HDIR  GL S ILIC++I NY  D SL  ++L  +G K+ + L+P    +D             N LKL L      +R++  N LL +++  + +E    I++   +  N++ +   +PYLGKN     ++  K       +  N K    RIL++ ++   P  + I+N FT  D   E   ++  +   S E++EL  I+++E++
Sbjct:  467 CLFIYSIKLKHSHIFDFNYRTIDDRLIEIGVKITDYLNQFCLNRNVKFLANHHISHSTFLSYD-----TQYFASHFIVLFEKLVYEIHLDCDKVYDSLSKIKTETCIEISPGLNLINGKITDKIIFEYFNKLKLSDDYILLNKTLCVAISDDCTQYMKDFLNIECMMKANVIFAIISPPKVHELLLVIDYSTEEHYYLSPVTTDLNVVSQHFCYNILIKINELRESKGRAMKTGHCRHDIRGSGLLSKILICAFIRNYALDYSLENINLHEIG-KIVNSLVPIVDGSDNVKYEKREKLKAANTLKLNL-----KQRKEKINQLLIKLSDSDVDEIICTILTNIPRLENLQKKEFREPYLGKN-----HNKPKKFNNPAEFLTNMKTTVYRILNDQSVKIEPPFQEIINNFTHPDVVPECWDNVLKFSKISDELLELTYISSEEIL 895          

HSP 5 Score: 71.2478 bits (173), Expect = 5.780e-12
Identity = 42/142 (29.58%), Postives = 72/142 (50.70%), Query Frame = -1
Query: 3317 DNITFSDGLFYATNKVTEFDFST--GCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPQEIRK----ETHHRRCTIRL 3724
            + I+F  G F   N+  + D S   G SDI+FN+G +NF+++   +   + +      +EKII++IF LN L+L+ ++   S   Y  +  NY+ NC  L+S+  ++      L+D + W P  ++       H   C  RL
Sbjct: 2010 EQISFVGGKFIKLNETKDSDHSQAEGESDIMFNIGLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERL 2151          

HSP 6 Score: 71.2478 bits (173), Expect = 5.780e-12
Identity = 42/142 (29.58%), Postives = 72/142 (50.70%), Query Frame = -1
Query: 3317 DNITFSDGLFYATNKVTEFDFST--GCSDILFNLGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSKLPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPQEIRK----ETHHRRCTIRL 3724
            + I+F  G F   N+  + D S   G SDI+FN+G +NF+++   +   + +      +EKII++IF LN L+L+ ++   S   Y  +  NY+ NC  L+S+  ++      L+D + W P  ++       H   C  RL
Sbjct: 4330 EQISFVGGKFIKLNETKDSDHSQAEGESDIMFNIGLLNFHEMCLKDTTLRSLFSQPKSSEKIIDIIFHLNYLQLKGELKHTSNGSYGDYVCNYVRNCLSLYSISDIETYNTLSLDDIEKWAPSLVKMIPPLSYHSSSCKERL 4471          
BLAST of SMED30010944 vs. Planmine SMEST
Match: SMESG000079542.1 (SMESG000079542.1)

HSP 1 Score: 573.933 bits (1478), Expect = 0.000e+0
Identity = 351/867 (40.48%), Postives = 504/867 (58.13%), Query Frame = -3
Query:  234 NAKLDGFAIDKYFQSLLDNDKYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLLVIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSPLHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQNTDQNILKLPLICKT-----INERRKICNILLAEIATCNANEAYSKIISAANKSNIKPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRPSMENILNQFTQMDPDEFVF-SISDYCIKSKEIIELDQITAKEVIFEYKQANASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIPKPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQKGGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIWNLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPISMILFNLAINPVLVAISSSPIAKYRMGDSEVQXXXXXXXXXXXANSATDLQALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGXXXXXXXXXXXKDKEFYHYLGVPVGECPNQTPYDTLYPWQKVDMYKTFLHSRLHFAFRTREIKISAIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFIDLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQCLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEY-DGFFSLRITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKL 2813
            N K+    +  +F +L  ++ +  +N T++ A+++D  Q++ EFL+   L+ A I+F I  PP + + L++IDY     +LLNP+S      ++ TA  LL  I+ ++       L+ +  HDIR  GL S ILIC++I +Y  D SL  ++L  +GN ++S LLP  Q+  ++ +K     KT     +++R  + N  L  +   N NE  +KI          P  K                  K K  +  N K    +IL    I+ RP++++ +  F+  DP    + ++ ++ +KS+  + L  I  KEVIFE K+A+ +SPG D I Y D+ + D EGKLL  L+N II    +P+ W SFKT++ PKP K   Y+  SSWRPIALLS  YKIF SILSRRLT W+  N LLH GQKGGS  +GCVEHN+VL++ LEHSKYSK  P+ IA+LDIKDAFGSVPH Y+W +L +IGV E F + L+LLYT+T+SYY CGP++TP I IK+GV+QGCP+SMILF +AINPVL A++ S +  +++G+S +Q+LAYADDIALIAN+  DLQ ++N + ++A  IG E+RP+KCAY+   N   I+          +  ++  Y  L   V +       D L  WQK+  YKTFLHSRL F FRTR+ KISA+   SA   +N   +T+LRS +    +LP+N+  +Y Y+ +E GGA+  DL DEY   TI   FRL T  C  T  +  DSL+FV   R+  + PS+     WIN     +    KK  + R R AI   + T  I I  E  D    LR+T   +GT I+   DR+ ++  LH  + ++Y+    N  +
Sbjct:   54 NCKITEKLLTNFFINLNLSENFIFLNNTMTTAILNDCTQYLNEFLNIENLMKAKIVFAIIAPPKARQILMIIDYTSDEYYLLNPSSLLPNFPYLCTANSLLTKINEIKETPELCKLLGNCPHDIRGSGLVSRILICAFIKHYALDLSLENINLRDIGNVIKS-LLPTVQDIIKDSIKNLSDNKTNVKLKLDQRILLINENLLTLQNANVNEMRNKISKV-------PHPK-----------------TKFKSSFNQNMKTTVYKILVNDEINERPTIKDHIGNFSHQDPLIKSYDNVLNFGLKSQNTLLLRPIIDKEVIFELKRADNTSPGIDGIKYNDIAIHDAEGKLLTLLYNKIITENTMPDVWKSFKTLMTPKPDKGDKYNLISSWRPIALLSVVYKIFASILSRRLTSWVNRNNLLHIGQKGGSVHDGCVEHNSVLSSCLEHSKYSKDTPIIIAFLDIKDAFGSVPHDYMWKILRHIGVGEKFTNTLKLLYTETSSYYTCGPIVTPNIPIKQGVKQGCPLSMILFAIAINPVLQAVTLSKVKPFKVGESSIQVLAYADDIALIANNTHDLQNILNIAFDVAREIGFEYRPKKCAYIQLPNVETIS----------EHTNQSPYEILNKVVSDAKKLADSD-LCGWQKLKAYKTFLHSRLTFPFRTRDFKISAL---SANVCNN---TTKLRSHLRKMMSLPNNSEISYFYNSVENGGASCTDLLDEYHTQTISYFFRLFTANCEFTKRINIDSLKFVTGPRLGNNNPSLQDSFNWINKADIIQKHSGKKPRFFRPRTAIQFFEKTHNITISFEVLDDKPILRLTTALKGTIILTEFDRRLVSKVLHLALYDSYFTKWKNSNI 878          
The following BLAST results are available for this feature:
BLAST of SMED30010944 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
CABZ01033394.11.918e-2324.34pep chromosome:GRCz11:13:6032844:6038477:-1 gene:E... [more]
CU462878.35.441e-2329.83pep chromosome:GRCz11:17:8542203:8545189:1 gene:EN... [more]
BX649434.31.168e-1929.55pep chromosome:GRCz11:3:18270043:18274691:-1 gene:... [more]
BX323564.11.505e-1824.94pep chromosome:GRCz11:2:10597810:10600950:-1 gene:... [more]
CU638714.11.977e-1829.60pep chromosome:GRCz11:24:19863246:19868789:1 gene:... [more]
back to top
BLAST of SMED30010944 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSXETT00000032317.19.723e-2633.20pep primary_assembly:Xenopus_tropicalis_v9.1:1:130... [more]
ENSXETT00000040456.11.018e-2532.78pep primary_assembly:Xenopus_tropicalis_v9.1:8:873... [more]
ENSXETT00000002428.12.683e-2332.80pep primary_assembly:Xenopus_tropicalis_v9.1:3:159... [more]
ENSXETT00000045854.16.959e-2230.65pep primary_assembly:Xenopus_tropicalis_v9.1:1:730... [more]
efcab144.198e-2132.43EF-hand calcium binding domain 14 [Source:Xenbase;... [more]
back to top
BLAST of SMED30010944 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q03274|PO22_POPJA1.208e-2228.57Retrovirus-related Pol polyprotein from type-1 ret... [more]
sp|Q03278|PO21_NASVI1.840e-2227.77Retrovirus-related Pol polyprotein from type-1 ret... [more]
sp|P16423|POLR_DROME6.980e-2125.11Retrovirus-related Pol polyprotein from type-2 ret... [more]
sp|O00370|LORF2_HUMAN6.927e-1728.15LINE-1 retrotransposable element ORF2 protein OS=H... [more]
sp|P08548|LIN1_NYCCO3.951e-1627.31LINE-1 reverse transcriptase homolog OS=Nycticebus... [more]
back to top
BLAST of SMED30010944 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A159X4Q60.000e+098.43ORF2-like protein (Fragment) OS=Schmidtea mediterr... [more]
A0A3L8D8E52.219e-6231.08Reverse transcriptase domain-containing protein OS... [more]
A0A3L8DAI71.051e-6130.61Reverse transcriptase domain-containing protein OS... [more]
K7JWY91.217e-6128.46Reverse transcriptase domain-containing protein OS... [more]
A0A3L8D7Z71.471e-6131.08Reverse transcriptase domain-containing protein OS... [more]
back to top
BLAST of SMED30010944 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSAMXT00000034560.11.652e-3127.05pep primary_assembly:Astyanax_mexicanus-2.0:APWO02... [more]
ENSAMXT00000034831.12.608e-2630.87pep primary_assembly:Astyanax_mexicanus-2.0:3:1621... [more]
ENSAMXT00000046127.17.433e-2132.53pep primary_assembly:Astyanax_mexicanus-2.0:7:1638... [more]
ENSAMXT00000033573.11.192e-2031.84pep primary_assembly:Astyanax_mexicanus-2.0:3:3140... [more]
ENSAMXT00000048276.11.806e-2029.55pep primary_assembly:Astyanax_mexicanus-2.0:17:785... [more]
back to top
BLAST of SMED30010944 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010944 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 2
Match NameE-valueIdentityDescription
EDO478461.551e-833.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
EDO359611.062e-632.94Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
back to top
BLAST of SMED30010944 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ENSORLT00000043450.11.503e-2230.10pep primary_assembly:ASM223467v1:12:7314868:731859... [more]
ENSORLT00000028663.11.568e-2227.11pep primary_assembly:ASM223467v1:7:26712747:267164... [more]
ENSORLT00000035976.11.633e-2232.14pep primary_assembly:ASM223467v1:16:7718738:772789... [more]
ENSORLT00000037544.14.270e-2226.88pep primary_assembly:ASM223467v1:22:28708275:28712... [more]
ENSORLT00000045921.14.482e-2233.33pep primary_assembly:ASM223467v1:18:29376025:29377... [more]
back to top
BLAST of SMED30010944 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000062936.12.313e-1890.70SMESG000062936.1[more]
SMESG000024308.10.000e+097.81SMESG000024308.1[more]
SMESG000021486.16.507e-9443.43SMESG000021486.1[more]
SMESG000021486.18.829e-9443.43SMESG000021486.1[more]
SMESG000079542.10.000e+040.48SMESG000079542.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010944 ID=SMED30010944|Name=SMED30010944|organism=Schmidtea mediterranea sexual|type=transcript|length=3781bp
TGGCTCTCATCAGTACTATGCCGGTAGGCGATCAGATTTCTGTTGTTTTC
AGGTTTCTCCTGGTGCCCTGCATGGGACTCATCTCATCACCCGTCTAGTT
TGCTAGCATTTATTGCCAAATTGTTTGTCCTAGCACGATGAACAAAGTTC
CAAGATACAGTGTTTAATCTACTTTTAAATATCGCTTTATTAATTAAAGG
GCACTTCACATAAGTATCAGCAATAAAATTATTAAGCTTTCCATTATGAA
GGTCCATATAATATGTTTCTTGTACAAGTTTATGGAGGAACGAAGCTAGT
TTTTTACGATCTAAACTGTAAATTATTGTAGTTCCCCTATGTTCAGCGGT
TATACGAAGCGAGAAAAAGCCATCATATTCAAGCAAGATATCGATTCCAC
GAGTGTTTTTCAAGTGAAGAATTGCATCACGAAACCTTATCCACCAAGTT
TTCTTTGACAAACTGTTTTTCTTGTAAGATTTACCATTTATCCATTCAAG
ACACTGAGATATTGACGGTCCAGAAATCCTGATGCGCGTCGAAGCAACAA
ATCTAAGGCTATCATGAATAACGGCAGATGTAATCGGACATTTACATGTC
AAAAGTCGGAAAGCTTGTACAATGGTCAGAATACTATATTCGTCATGTAA
ATCAATGAAAGCTGCACCCCCAGTCTCAATCGGACTGTAAATATATGCTT
TAGGAGCATTATGCGGGAGGTTAAAAAAACTGTATATAATAGATCTGAGT
TGTGTGCTCAGATTCTTATTATGGCTATCTTTGGCTGATGGTCTACCAAT
AGCGCTGATTTTTATCTCCCTAGTTCTGAAAGCAAAATGAAGGCGAGAAT
GGAGAAATGTTTTGTACATATCTACCTTCTGCCAAGGATACAATGTATCA
TATGGAGTTTGATTGGGACACTCACCAACTGGAACACCGAGGTAATGGTA
AAATTCTTTATCTTTCAGCTTTTTCAATTTAACATTATTCAAATTTACAC
CATTTATGTCAGTGGAATTTGACGTTGACAAGTAGGCGCATTTCTCTGGC
CTGAATTCCAGGCCAATCCTACTTGCAATCTCAACTGAATTGTTAATAAG
CGCTTGCAAATCAGTAGCACTGTTGGCAATCAACGCAATGTCGTCCGCGT
ATGCCAGAATCTGCACTTCGGAATCACCCATTCTATATTTCGCTATAGGC
GATGAGGAGATTGCAACCAAAACTGGGTTTATGGCCAAGTTAAAGAGAAT
CATTGATATTGGACAGCCCTGACGCACCCCTCGTTTGATATCAATTTTAG
GGGTAATCAAAGGTCCGCAACAGTAGTATGAATTAGTCTTTGTATACAAG
AGGCGCAGAACGGAAACAAAATGTTCATCAACCCCTATATATGCCAGAAG
ATTCCATATGTATGCATGTGGTACACTTCCGAATGCATCTTTAATGTCCA
GCCAAGCGATTGCCAACGGGGCTTTTTTCGAGTATTTAGAATGTTCAAGG
ACAGCAGTCAAAACTGCATTGTGCTCAACACAACCTTCAAATCTAGACCC
TCCTTTTTGGCCTGGGTGTAACAGTTTATTCTCAATTATCCATTTAGTTA
GTCGCCTAGACAGAATTGAGGTAAATATTTTATAAGAGGTAGACAAAAGT
GCGATTGGCCGCCACGAAGATGTGTCGTCATAATCACCCGACTTTCCCGG
TTTTGGAATTAAGATGGTTTTAAAACTTAGCCAGTCATTCGGAATCTGAC
CACTATCTATGATTATATTAAACAGTCGCGCTAATAGTTTACCCTCGGGG
TCTAAAAGACGGAGATCTCTATAAGTTATGTTATCATCTCCAGGAGAAGA
AGCATTCGCCTGTTTATATTCAAAGATCACCTCCTTTGCAGTTATTTGAT
CTAGTTCAATAATCTCCTTAGACTTTATGCAGTAGTCACTTATTGAGAAG
ACGAACTCATCAGGATCCATTTGCGTAAATTGATTGAGGATATTTTCCAT
ACTCGGTCTTGTGTCAATATTAATCTCATCCAAAATCCTAGTGGCAACCT
TCTTACGGTTGTGGAAATACCCCCCTTTTATTTTAATCATTTTATTGTCA
CTATTTAATGAGTTAAAGAAATTTTTACCCAAGTATGGTTTATGCTCAGG
TTTTATGTTACTTTTATTTGCCGCAGAAATTATTTTAGAATAGGCCTCAT
TTGCGTTACATGTTGCAATTTCGGCTAACAATATATTACAAATTTTACGG
CGCTCATTAATAGTTTTACAAATTAACGGTAATTTTAGTATATTTTGATC
AGTATTTTGCTTAGGTGGCAGAAGTATACTTTCCAATTTGTTTCCGACAT
ATGTCAGATCCATTTTAAAAAGGCTTCTGTCAGTCAGGTAATTTGTGATA
TAGCTGCATATGAGAATATTAGAAAAAAGACCACAAGTACGAATATCATG
TAGTGGACTAGTCATTAGTGGTGCAGCTCCAACGTTGTTTCGAACAGATG
AGATGTAGTTCAGGAGGATGAATGCAGTCTCGACATGTAAAGGAATTTCC
TTTGAAGACGTGGGATTAAGCAAAAAGGAAGTGCACTTTTCATAATCGAT
AACTAGTAGTTGCTTCCCAGAAGATGGAGGAACAAAAATTACAAAAATAA
TGGATGCATCCAATAGCTTAGTTTTGTCAAGGAATTCAGCCACATGCTGC
CAACTGTCACTAATCAAGGCATCACTCAACGTTTCATTCACCAAAAAGTA
CTTATCATTATCTAAAAGACTTTGGAAATACTTGTCAATGGCAAAACCAT
CCAGCTTTGCATTTATCCCTATCACATTCAAGGTTGTTAGTGAAATTTTA
TTTGATTTTAAGGAATTGGGAATGTAACGCCCAACCTCGCAGTCAATCCA
GTCATCAGAAATATTATTATTGTTTATCGAATTAATGATAATTTTAAAAG
ATGCTTTACAAACATTCAAATCATCATACTCCATAAACTGGGCTTTACTA
TAGTCCGTTGACATTTGAACTATTTCAGAACGGCAAAATTGATTAAAAAG
CATTGTTAAAGTTTCACCAATGATTTTTAAATTAGATAAAAGAAAAATAT
ATATATTACATCATTGGATCTAAAGGGACAAACTATATAAGCAATTTCCT
TGAACTGTTTATAAGTGATGTTATCTATTATGAAGGTTTCATTTATAACA
ATCAAGTTGTCACTAGCTACTTCATCTAAAACAAAAGAAGCAGGTCCCCT
GCCAACAAGTCAATATTCTTCGTCACCAATGATGTCGCCAGATGCCCCAC
TAGCAGCATCCCCGTGAAGCCTGATCGTGCATCTACGATGATGCGTTTCT
TTACGGATTTCCTGGGGTCCCCACGTTTGGAAATCCTCAAGACGATAATT
TGCTAATTCATCAACAGACAAGACTGAGTGAAGTACACCACAATTCAGAA
TGTAATTATAGGCAAATCTATGATAAGGCAGCTTTGAATTTTGTGCGATT
TTATTTTCCAATTTCAAACAATTTAATAAAAATATCATATTAATAATCTT
CTCATTGATATTTGAAATTTTTAAAATTTCTTGGAAGTCTTTATTTGATT
TAGAAATTTTATAAAAATTGACATGGCCAAGATTAAAAAGAATATCAGAG
CAACCAGTCGAAAAGTCAAATTCAGTCACTTTATTTGTAGCATAAAAGAG
ACCATCAGAAAACGTGATATTATCTATATTATTACGCAATAATATTTTAT
TTATGGACTTGTCTGAATTTTCACCGGTCTT
back to top

protein sequence of SMED30010944-orf-1

>SMED30010944-orf-1 ID=SMED30010944-orf-1|Name=SMED30010944-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=174bp
KTGENSDKSINKILLRNNIDNITFSDGLFYATNKVTEFDFSTGCSDILFN
LGHVNFYKISKSNKDFQEILKISNINEKIINMIFLLNCLKLENKIAQNSK
LPYHRFAYNYILNCGVLHSVLSVDELANYRLEDFQTWGPQEIRKETHHRR
CTIRLHGDAASGASGDIIGDEEY*
back to top

protein sequence of SMED30010944-orf-2

>SMED30010944-orf-2 ID=SMED30010944-orf-2|Name=SMED30010944-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=989bp
MLFNQFCRSEIVQMSTDYSKAQFMEYDDLNVCKASFKIIINSINNNNISD
DWIDCEVGRYIPNSLKSNKISLTTLNVIGINAKLDGFAIDKYFQSLLDND
KYFLVNETLSDALISDSWQHVAEFLDKTKLLDASIIFVIFVPPSSGKQLL
VIDYEKCTSFLLNPTSSKEIPLHVETAFILLNYISSVRNNVGAAPLMTSP
LHDIRTCGLFSNILICSYITNYLTDRSLFKMDLTYVGNKLESILLPPKQN
TDQNILKLPLICKTINERRKICNILLAEIATCNANEAYSKIISAANKSNI
KPEHKPYLGKNFFNSLNSDNKMIKIKGGYFHNRKKVATRILDEINIDTRP
SMENILNQFTQMDPDEFVFSISDYCIKSKEIIELDQITAKEVIFEYKQAN
ASSPGDDNITYRDLRLLDPEGKLLARLFNIIIDSGQIPNDWLSFKTILIP
KPGKSGDYDDTSSWRPIALLSTSYKIFTSILSRRLTKWIIENKLLHPGQK
GGSRFEGCVEHNAVLTAVLEHSKYSKKAPLAIAWLDIKDAFGSVPHAYIW
NLLAYIGVDEHFVSVLRLLYTKTNSYYCCGPLITPKIDIKRGVRQGCPIS
MILFNLAINPVLVAISSSPIAKYRMGDSEVQILAYADDIALIANSATDLQ
ALINNSVEIASRIGLEFRPEKCAYLSTSNSTDINGVNLNNVKLKKLKDKE
FYHYLGVPVGECPNQTPYDTLYPWQKVDMYKTFLHSRLHFAFRTREIKIS
AIGRPSAKDSHNKNLSTQLRSIIYSFFNLPHNAPKAYIYSPIETGGAAFI
DLHDEYSILTIVQAFRLLTCKCPITSAVIHDSLRFVASTRIRISGPSISQ
CLEWINGKSYKKNSLSKKTWWIRFRDAILHLKNTRGIDILLEYDGFFSLR
ITAEHRGTTIIYSLDRKKLASFLHKLVQETYYMDLHNGKLNNFIADTYVK
CPLINKAIFKSRLNTVSWNFVHRARTNNLAINASKLDG*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000099neuron
PLANA:0000101muscle cell
PLANA:0000103central nervous system
PLANA:0000142posterior region of the whole animal
PLANA:0000418head
PLANA:0002075pigment cell
Vocabulary: INTERPRO
TermDefinition
IPR000477RT_dom