G_PROTEIN_RECEP_F1_2 domain-containing protein

Overview
NameG_PROTEIN_RECEP_F1_2 domain-containing protein
Smed IDSMED30010926
Length (bp)1217
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010926) t-SNE clustered cells

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010926) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010926) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for G_PROTEIN_RECEP_F1_2 domain-containing protein (SMED30010926) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30010926

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 1

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
prepharyngeal regionSMED30010926h1SMcG0021571 dd_Smed_v6_79653_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017
whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Match: frpr-12 (FMRFamide Peptide Receptor family [Source:UniProtKB/TrEMBL;Acc:Q9GYH3])

HSP 1 Score: 51.6026 bits (122), Expect = 5.500e-7
Identity = 74/337 (21.96%), Postives = 143/337 (42.43%), Query Frame = 3
Query:   45 PNMTQEKELSLIKFNFISLFCIGM-PLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMIN----VCEMFRNLLTVLIGIERYLVTCHPLNI----LGTNKFRSAIY--GISICFFLTVLLRVPIFLRTVISNANVSDYA-----YKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKDTRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAA-----------ICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKC 974
            PN T     ++  +    +F + M P+I++G+  + ++  ++  +     T    L  LS +D++ L  A+        + ++P   ++        + +A  ++ I     + +M    + VLI +ER+   C PL +       N  R+ +Y   ISI F     L     L T + N  +S  A     + ++  I SIF   L      F  I +TN +   + ++ S+E        Q K+ + T ML++++ +++ C          +N + K  F+N ++  +A            I  L  + YS S  F+Y+I+  KYR ++   + C
Sbjct:   35 PNSTDFPTSNVQSYTLNEVFSMVMLPIILIGLLGNTVSIYVYSRNHMKKNTIGFLLLSLSTIDLIVLVTALPTFG-TYKFPFFPGYNKIGSA---HTIFSAFCLVYIYPFGCMAKMIGQYIIVLIAVERWFAVCRPLQVQVWCTQKNTIRAMLYIIAISISFNAPRFLEFTADLSTGVVNMGLSHSANNTWYFYLYYGIRSIFFDALIP----FAIISVTN-IQVIQQLHKSNEERKLLTTQQQKEKRTTTMLLVMVILYATCHF--------FNTTLK--FINMVSKTYAQFRFPVIRVIHHISNLLLVIYSASTVFIYLIFSTKYRKVLSTCVTC 352          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3S5C4G3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Protopolystoma xenopodis OX=117903 GN=PXEA_LOCUS28147 PE=4 SV=1)

HSP 1 Score: 139.428 bits (350), Expect = 3.564e-32
Identity = 111/328 (33.84%), Postives = 172/328 (52.44%), Query Frame = 3
Query:  108 IGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDL-IELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRSAIYGISICFFLTVLLRVPIFL------RTVISNAN--VSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYT------SSENTPQ---------------RLKSQIKKIKDTRMLVIIISIFSLCGLPSIIYTI--------IYNASKKCNFVNEITTR-FAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKC 974
            +G+P+++ G+  S ++  MF  DR TP+TT++ L   + +D  FL +A  Y+E K +   YP L    L       V     II IN CE+ RN + VLIG+ERY++TC+PL+       R+   GI++C    +LLR+P+ L      RT +S+    V   + +IHT+IDSI L +LPVL+L FCS++I   ++ S  + +      S  N                  RL +QI+  K  ++L+I++  F+ CGLP + +           + A +K +  +E +      +CW  S+  ST+NFFVY+IY  KYR I   ML C
Sbjct:  202 LGLPVLLTGLITSLLSLGMFFRDRLTPRTTRIMLTTSAGLDFAFLLSAAFYLEFKTILSQYPSLHARRLYDAYEDYV-----IIFINYCELLRNWVIVLIGLERYIITCYPLHCKAWWSVRTTKIGIAVCAAFALLLRLPMLLRRIARKRTHLSSWWQLVHSESLRIHTLIDSICLTLLPVLILSFCSLQILRVMHTSTRLRSNHLLGGSGSNRGSISAGHKGEQIRLNRIRLANQIRA-KVHKVLMIVLGTFTFCGLPFLPHIFFECYREWETFQAREKASCWSETSYNILTPVCWFCSMLNSTANFFVYIIYWDKYRQIFHTMLGC 523          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P7NP63 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS6860 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 2.157e-30
Identity = 97/315 (30.79%), Postives = 165/315 (52.38%), Query Frame = 3
Query:  108 IGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPL--NILGTNKFRSAIYGISICFFLTVLLRVPIF----LRTVISNANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLK----------SQIKKIKDTRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRF------AAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCHCHK 986
            +G+P+ +VGI  S ++  +F  D+ TP+TT   L + S++D+ FL  ++LY++     +    L  LL        L  I    +N+ E FRN L VLIG+ER+LV C P+   +  T K   ++  ++ CF  +V++R+P+     L +V    +V+ + Y++H+  DSI + ++P+++L+ CS++I   L  S          P+R +          S  +++K TR L+I+I  F+L  LP +  +I+   S   +F     T F      + +  L S   ST+NFFVY++Y  KYR ++  ML C   K
Sbjct:   40 LGLPVCMVGIVTSGLSICLFCRDKTTPRTTCKLLIVTSLIDVQFLLFSLLYLQPLTFCKRSSPLRRLL--------LTNIIFSFVNILECFRNWLVVLIGVERFLVICFPVRSKVWWTGKITDSL--MAACFVFSVVVRLPLISYLSLESVKPKGSVTAWLYQLHSFTDSILVTLIPLIILIVCSLQIGRGLQQSDHFRWGQGKHPERQRASTSSTSSNCSVARRVKLTRGLLIVIVTFTLFMLPMVPVSIVQLLSH--HFFTSSCTYFITLHVCSYVATLGSQMNSTANFFVYIVYWGKYRKMLKRMLGCQYAK 342          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P7KZU3 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS5855 PE=4 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 1.345e-28
Identity = 101/347 (29.11%), Postives = 174/347 (50.14%), Query Frame = 3
Query:   45 PNMTQEKELS------LIKFNFISLFCI-GMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIE-LKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLN--ILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISN-----ANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLK--------------SQIKKIKDTRMLVIIISIFSLCGLPSIIYTIIYNASKK---CNFVNEITTRFAA-ICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCHCHK 986
            P M QE  L        +++N + L  I G+P+ + GI  S ++  +F  D+  P+TT+  L + S++D+ FL  ++ Y++ L    +  P  + LL         N I  +++N+ E FRN L VLIG+ER+LV C P++  +    K    + G   CF  ++++R+P+     I N     + V+++ Y++H+  DSI + ++P+++L+ CS++I   L  S          P+R                S  +++K TR L+I+I  F+   LP +  +I+   S      N    IT  F + +  L S   ST+NFFVY++Y  KYR ++  +L C   K
Sbjct:   12 PCMMQEAWLGGPTHEDFLRYNRLILLSILGLPVCVAGIVTSGLSICLFCCDKKAPRTTRKLLIVTSLIDVQFLLLSLFYLQPLTFCKRTSPLRLPLL---------NNILFVLVNILECFRNWLVVLIGLERFLVVCFPVHSKVWWNGKKTDGLIG--GCFGFSIVVRLPLISYLAIENVKPEWSGVTEWFYQLHSFTDSILVTLIPLIVLIVCSLQIGRGLRRSDRFRLGPREYPERQTLSTVSQKQNTSSKCSIARRVKLTRGLLIVIFTFTFFMLPMVPVSIVQLLSHHFFTSNCAYLITVHFCSYVAALGSQMNSTANFFVYIVYWGKYRKMLKRVLGCQYEK 347          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0R3VTU0 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Taenia asiatica OX=60517 GN=TASK_LOCUS680 PE=4 SV=1)

HSP 1 Score: 120.553 bits (301), Expect = 7.612e-27
Identity = 94/308 (30.52%), Postives = 162/308 (52.60%), Query Frame = 3
Query:  108 IGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHP------LNILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISN-----ANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIY--------TSSENTPQRLKSQIKKIKDTRMLVIIISIFSLCGLPSIIYTII----YNASKKCNFV--NEITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIII 956
            +G+P+ ++GI  S ++ ++F+ D  TP TT+  L  +SI D VFL +++LY++           I  L PV            ++N+ EM RN   VLI IERY++TCHP      L++  TNK       I+IC   TVL R+P  +   + +     A+ +    +IH+ ID++ + +LP+ +++ CS+RI   +  S+ ++        + S +T +RLK +   +K TR+L+++I +F++  LP +   I+    +     C+ +   +I    A    L SI +S +NF +YVI   KYR I+
Sbjct:   43 LGLPVSLIGIATSIVSLVLFLRDHLTPHTTRHLLLTMSIADAVFLLSSLLYLQTMNFCGRSCRRIAYLLPVST----------LVNIFEMLRNWTVVLICIERYIITCHPLRSKRWLSLPRTNK------SIAICVIATVLARIPFMVYVTLESYGATFASTTHLLKQIHSTIDAVLVTLLPLFIIVVCSLRIYRCIQDSEMLHRMVVVVAASHSASTRRRLKYRSNSVKVTRVLLVVIVVFTILMLPLVPVNILAFEQFFPGATCHVLIGRQIVDPLAV---LGSILHSMANFLIYVICWQKYRRIL 331          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A3P7LR68 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Dibothriocephalus latus OX=60516 GN=DILT_LOCUS11551 PE=4 SV=1)

HSP 1 Score: 116.701 bits (291), Expect = 1.327e-25
Identity = 104/344 (30.23%), Postives = 171/344 (49.71%), Query Frame = 3
Query:   45 PNMTQEKELS------LIKFNFISLFCI-GMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPL--NILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISN-----ANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKD--------------TRMLVIIISIFSLCGLPSIIYTII---YNASKKCNFVNE--ITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCH 977
            P M Q+  L        +++N + L  I G+P+ +VGI  S ++  MF  D+ TP+TT+  L +LS++D       VLY+ L +L     +     CP +   +L +I + +  + E FRN L VLIG+ER+LV C P+   +  T    + +  I++CF  ++L+R+P+   T I N     + V    +++H   DSI + ++P+++L+ CS++I   L  S          PQ   + I   KD              TR L+I+I  F+L  LP  I  ++   +   + C ++    I +   A   L S   ST+NFFVY++Y  KYR ++  ML C 
Sbjct:   12 PCMLQKAWLGGPTYKDFLRYNQLILITILGLPVCVVGIFTSGLSICMFGRDKTTPRTTRKLLIVLSVLD-------VLYLLLSMLVFQPMNFCGRNCP-QQMPLLTSIIVPLGTILESFRNWLVVLIGVERFLVICFPVRSKVWWTGAITNRL--IAVCFGFSILVRIPLISFTAIENVRPEWSAVKSRFWQLHCFTDSILVTLVPLIILIVCSVQIGRGLRRSGRFRRGQGEHPQLQTASIDCQKDGIQSDCIVLRCLKLTRGLLIVIITFALFMLPLSILQLLSLHFLRHRTCVYMVSLNICSYLVA---LGSQINSTANFFVYIVYWAKYRRMLRQMLGCQ 342          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000015663.1 (SMESG000015663.1)

HSP 1 Score: 703.745 bits (1815), Expect = 0.000e+0
Identity = 364/365 (99.73%), Postives = 364/365 (99.73%), Query Frame = 3
Query:   51 MTQEKELSLIKFNFISLFCIGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISNANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKDTRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCHCHKYSSITTPFRLIAPVIKMRNLNNKDILETSNRLRDAKKIGGIILFEHALKLENQ 1145
            MTQEKELSLIKFNFISLFCIGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISNANVSDYAYKIHTIIDSIFLQILPVLLLLFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIK TRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCHCHKYSSITTPFRLIAPVIKMRNLNNKDILETSNRLRDAKKIGGIILFEHALKLENQ
Sbjct:    1 MTQEKELSLIKFNFISLFCIGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRSAIYGISICFFLTVLLRVPIFLRTVISNANVSDYAYKIHTIIDSIFLQILPVLLLLFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKVTRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCHCHKYSSITTPFRLIAPVIKMRNLNNKDILETSNRLRDAKKIGGIILFEHALKLENQ 365          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000058691.1 (SMESG000058691.1)

HSP 1 Score: 196.438 bits (498), Expect = 4.326e-59
Identity = 133/337 (39.47%), Postives = 198/337 (58.75%), Query Frame = 3
Query:   84 FNFISLFCIGMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRS---AIYGISICFFLTVLLRVPIFLRTVI---SNAN---VSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKDTRMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLK----CHCHKYSSITTPFRLIAPVIKMRNLNNK 1055
            FN + LF +G P+I+ GI  S ++ IMF+ D FTP++TK  L+ LSI D++FL + + Y ELK++Y  YP L   L P++ + +     ++++N+ E+FRN LTV+IGIER+LVTC+PL +     F +   A++ I I    T LLR+P+ +R +    +N +   +S  + K H II++IF   LPV +LLFC IRIT AL  SK +     ++   +K   +K+  TRML+III +FS C  P I+  I     ++   +N       +ICW FSI YS+SNFFVY+IYM +YR+II  M      C      S+   ++LI       NL N+
Sbjct:   18 FNQLFLF-LGAPIILTGIISSILSTIMFLRDSFTPRSTKFILQTLSITDLLFLMSMIFYFELKIIYILYPHLELTLKPIKEKFIFADFLVMLVNISELFRNWLTVIIGIERFLVTCYPLKMTKYQDFNNSYLAVFSIGI---FTFLLRLPMLIRRITFYSTNQHMIQISQKSLKAHDIIEAIFQSFLPVTILLFCFIRITKALKKSKALRIFHRSSAVSIK---RKMHITRMLIIIIILFSFCNTPHILNAIF----QQLKIINR---NLISICWFFSILYSSSNFFVYIIYMKRYRLIISLMFSNKFCCPLTTIESLEAKYQLINSYRDRSNLRNR 340          
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000081403.1 (SMESG000081403.1)

HSP 1 Score: 152.91 bits (385), Expect = 7.182e-42
Identity = 112/328 (34.15%), Postives = 170/328 (51.83%), Query Frame = 3
Query:  111 GMPLIIVGITFSCMTFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYY--PDLIELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILGTNKFRSAIYGISICFFLTVLLRVP--IFLRTVISNANVSDYAYKIHTIIDSIFXXXXXXXXXXFCSIRITNAL-NASKDIYTSSENTPQRLK----------------SQIKKIKDT--RMLVIIISIFSLCGLPSIIYTIIYNASKKCNFVNE----------------ITTRFAAICWLFSISYSTSNFFVYVIYMPKYRIIIGHMLKCH 977
            GMPLI VGI  S ++F MF+ D  TPKTTK  L + +++D++FL  A  Y+ELKVL   +   D+ +     +  +       I++N CEMFRN +TV+IG+ERYL+ CH L+       +    GI IC  ++V+LR+P  I + T     N+S      H  ID+IF+ I P+ +LL CS  I   + N S ++  SS+                       S+I +++    +ML++++ IF++CG P II  ++   +   +  N+                ++   + +CW  SI YST+NFFVYV Y  KYR II  ML C 
Sbjct:   26 GMPLIAVGIFSSLLSFGMFLKDNMTPKTTKTMLMVTAMLDLIFLILAFFYLELKVLSVTFNLTDIDQFYNQTKVPVS------ILLNWCEMFRNWITVMIGLERYLIICHALDFKKWWNLKVTRLGILICSLISVILRIPHLIGVTTKDKYQNISLLLMATHDWIDAIFMTITPIFILLVCSFAIAKQIRNLSANLSMSSKTKETHDNVRASSISISRSQFNNTSRIDRVRTRVHKMLMVVLIIFTVCGTPFIINAVVRIIAGDLSLFNQNITSNPNPIDQECSKVVSKILSVLCWFCSIIYSTTNFFVYVCYFKKYRQIIHRMLVCQ 347          
The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 1
Match NameE-valueIdentityDescription
frpr-125.500e-721.96FMRFamide Peptide Receptor family [Source:UniProt... [more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A3S5C4G33.564e-3233.84G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P7NP632.157e-3030.79G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P7KZU31.345e-2829.11G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A0R3VTU07.612e-2730.52G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
A0A3P7LR681.327e-2530.23G_PROTEIN_RECEP_F1_2 domain-containing protein OS=... [more]
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 3
Match NameE-valueIdentityDescription
SMESG000015663.10.000e+099.73SMESG000015663.1[more]
SMESG000058691.14.326e-5939.47SMESG000058691.1[more]
SMESG000081403.17.182e-4234.15SMESG000081403.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010926 ID=SMED30010926|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1217bp
CAAAAATATGTTTGAAGCATATGAATAATTATCAAAAAAATTGTCCAAAC
ATGACTCAAGAAAAAGAATTGTCTTTGATAAAATTTAATTTTATTTCCCT
CTTTTGTATTGGAATGCCATTAATTATCGTTGGGATTACATTCAGTTGCA
TGACTTTTATCATGTTCGTTTTGGACAGATTCACTCCGAAAACTACGAAA
TTATTCCTGAAAATTTTGTCTATTGTCGACATTGTGTTTTTGGCTAATGC
CGTCCTTTATATTGAATTGAAAGTTCTTTATCAGTATTATCCCGATCTCA
TAGAGTTGTTATGTCCAGTAAGAAATCAAATTGTTTTAAATGCTATTAAT
ATAATTATGATTAATGTCTGTGAAATGTTTCGAAATCTCTTGACGGTTTT
GATTGGAATTGAAAGGTATTTAGTTACATGTCATCCGTTAAATATTTTAG
GAACAAATAAATTCCGGTCCGCAATATATGGAATATCTATTTGCTTTTTT
TTGACAGTTTTATTGCGCGTTCCTATTTTCTTGAGAACTGTCATTTCGAA
TGCAAATGTGTCAGATTACGCTTACAAAATTCACACTATCATTGACTCGA
TATTTTTGCAAATTTTGCCAGTCTTGCTGTTATTGTTTTGTTCAATCAGA
ATCACAAATGCGCTTAATGCATCGAAAGATATTTATACGAGCTCCGAAAA
TACTCCACAAAGACTAAAATCTCAAATTAAAAAAATTAAAGATACTAGGA
TGTTAGTTATTATCATATCAATATTCAGTTTATGTGGTTTGCCATCAATT
ATTTACACAATTATTTATAATGCGTCTAAAAAATGCAATTTTGTCAATGA
GATAACAACAAGATTTGCGGCAATTTGCTGGTTATTTAGTATTTCGTATT
CAACGAGCAATTTTTTTGTTTATGTCATTTATATGCCTAAATATCGAATA
ATTATTGGCCATATGTTAAAATGCCATTGCCACAAATACAGTTCAATAAC
TACACCATTTAGATTGATTGCTCCAGTTATAAAGATGCGAAATTTGAACA
ATAAAGATATTTTGGAAACATCAAACAGGTTAAGAGATGCTAAAAAAATC
GGTGGAATCATTCTCTTTGAGCATGCATTAAAATTAGAAAATCAAGATGA
TCATAGCGATGATTCGGATTGAAAGTGAACGTTGTGTTATGATCAGTTCA
ATTTTTTTCCATAAAAT
back to top

protein sequence of SMED30010926-orf-1

>SMED30010926-orf-1 ID=SMED30010926-orf-1|Name=SMED30010926-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=390bp
KICLKHMNNYQKNCPNMTQEKELSLIKFNFISLFCIGMPLIIVGITFSCM
TFIMFVLDRFTPKTTKLFLKILSIVDIVFLANAVLYIELKVLYQYYPDLI
ELLCPVRNQIVLNAINIIMINVCEMFRNLLTVLIGIERYLVTCHPLNILG
TNKFRSAIYGISICFFLTVLLRVPIFLRTVISNANVSDYAYKIHTIIDSI
FLQILPVLLLLFCSIRITNALNASKDIYTSSENTPQRLKSQIKKIKDTRM
LVIIISIFSLCGLPSIIYTIIYNASKKCNFVNEITTRFAAICWLFSISYS
TSNFFVYVIYMPKYRIIIGHMLKCHCHKYSSITTPFRLIAPVIKMRNLNN
KDILETSNRLRDAKKIGGIILFEHALKLENQDDHSDDSD*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000419prepharyngeal region
Vocabulary: INTERPRO
TermDefinition
IPR015155PFU
IPR000276GPCR_Rhodpsn
IPR017452GPCR_Rhodpsn_7TM
Vocabulary: biological process
TermDefinition
GO:0007186G-protein coupled receptor signaling pathway
Vocabulary: cellular component
TermDefinition
GO:0016021integral component of membrane
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0004930G-protein coupled receptor activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000276G protein-coupled receptor, rhodopsin-likePRINTSPR00237GPCRRHODOPSNcoord: 247..271
score: 27.27
coord: 289..315
score: 18.42
coord: 65..86
score: 21.0
coord: 195..218
score: 20.2
IPR000276G protein-coupled receptor, rhodopsin-likePFAMPF000017tm_1coord: 50..307
e-value: 4.0E-14
score: 52.6
NoneNo IPR availableGENE3DG3DSA:1.20.1070.10coord: 16..334
e-value: 2.5E-28
score: 100.9
NoneNo IPR availablePANTHERPTHR46641FAMILY NOT NAMEDcoord: 13..327
NoneNo IPR availableCDDcd149787tmA_FMRFamide_R-likecoord: 36..315
e-value: 4.36024E-17
score: 78.8288
NoneNo IPR availableSUPERFAMILYSSF81321Family A G protein-coupled receptor-likecoord: 23..331
NoneNo IPR availableTMHMMTMhelixcoord: 250..272
NoneNo IPR availableTMHMMTMhelixcoord: 287..309
NoneNo IPR availableTMHMMTMhelixcoord: 67..89
NoneNo IPR availableTMHMMTMhelixcoord: 156..178
NoneNo IPR availableTMHMMTMhelixcoord: 30..52
NoneNo IPR availableTMHMMTMhelixcoord: 193..215
IPR017452GPCR, rhodopsin-like, 7TMPROSITEPS50262G_PROTEIN_RECEP_F1_2coord: 47..307
score: 21.415