39S ribosomal protein L10, mitochondrial

Overview
Name39S ribosomal protein L10, mitochondrial
Smed IDSMED30010905
Length (bp)3172
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 39S ribosomal protein L10, mitochondrial (SMED30010905) t-SNE clustered cells

Violin plots show distribution of expression levels for 39S ribosomal protein L10, mitochondrial (SMED30010905) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 39S ribosomal protein L10, mitochondrial (SMED30010905) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 39S ribosomal protein L10, mitochondrial (SMED30010905) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30010905

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 9

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
Smed sexual biotypeSMED30010905h1SMcG0019147 Contig5146GPL15192PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
cephalic gangliaSMED30010905h1SMcG0019147 dd_Smed_v4_8959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parapharyngeal regionSMED30010905h1SMcG0019147 dd_Smed_v4_8959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymaSMED30010905h1SMcG0019147 dd_Smed_v4_8959_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010905h1SMcG0019147 dd_Smed_v4_7627_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010905h1SMcG0019147 dd_Smed_v4_7627_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010905h1SMcG0019147 dd_Smed_v4_7627_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30010905h1SMcG0019147 dd_Smed_v6_8959_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
head regionSMED30010905h1SMcG0019147 OX_Smed_1.0.11558ox_Smed_v2PMID:24238224
Kao et al., 2013		whole organism asexual adult RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. UniProt/SwissProt
Match: sp|Q550F6|DCX_DICDI (Protein doublecortin OS=Dictyostelium discoideum OX=44689 GN=dcx PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 2.060e-6
Identity = 31/102 (30.39%), Postives = 54/102 (52.94%), Query Frame = 2 
Query: 1682 HSVDFFSP*MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLF-ELTGTLVQSHTQIVDGNYYVAASSENF 1984
            HS+D FS          +   ++K+I  +RNGD Y     V +   +    +++ + + ++V+LP   P+RKL+   +G LV++  +I+DG YYV A  E  
Sbjct:  181 HSIDKFS----------VQTEKAKVIMCFRNGDRYHSGERVTVHSTKFKTYDQLKEQLSKQVKLPTG-PVRKLYLASSGKLVKTMEEIIDGEYYVCAGGETL 271
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Match: A0A094ZYL2 (39S ribosomal protein L10, mitochondrial OS=Schistosoma haematobium OX=6185 GN=MS3_08526 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 2.893e-28
Identity = 77/229 (33.62%), Postives = 123/229 (53.71%), Query Frame = 2 
Query:   89 QNVRHRFNEDPGTLEQRLFRAVTEAVVP---FKPRDMLINSSXXXXXXXXXXXXXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNS--LQEAHANLVRNLSSCQ-ADMATNLQFHQISLISGLDELASRNK 757
            Q++R R+  DPGTLEQRLFR VT+ V+P     P +  +   +  +   +       FL+E+ +ED ++     M+LVF    HK+  +  +K+ LF   M F  +P  I++ A+KGT        +    + NM +  + + E  R A+   +KV  LLL+GGI+DYRI TV Q+++F AL+S  L  A ++LV  L   +   +  +L +HQ  +++GL  L    K
Sbjct:   20 QSIRFRYVPDPGTLEQRLFREVTKPVIPPINVNPVEQRLRRQEENRLRQENNPYRK-FLIERAKEDFLKPMNNNMVLVFQQLCHKAREMVPVKNKLFLKDMHFKGFPLSILREASKGTRYEMFTRYMLHSNIPNMYLFSEPTPEKCRYAINITKKVHFLLLLGGIVDYRIMTVQQLNEFAALSSQGLDGARSHLVMLLEQNRGGQIVQDLNYHQSFIVNGLKNLYDETK 247
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Match: A0A3Q0KIQ7 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 3.504e-28
Identity = 77/229 (33.62%), Postives = 123/229 (53.71%), Query Frame = 2 
Query:   89 QNVRHRFNEDPGTLEQRLFRAVTEAVVPF---KPRDMLINSSXXXXXXXXXXXXXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNS--LQEAHANLVRNLSSCQAD-MATNLQFHQISLISGLDELASRNK 757
            Q+VR R+  DPGTLEQRLFR VT+ V+P     P +  +   +  +   +       FL+EK +ED ++     M+LVF   YHK+  +  +K+ L    M F  +P  I++ A+KGT        +    + N  +  + + E  R A+   +K   LLL+GGI+DYRI TV Q+++F AL+S  L  A ++LV  L   + + +  +L +HQ  +++GL  L+   K
Sbjct:   20 QSVRFRYVPDPGTLEQRLFREVTKPVIPLINVHPVEQRLRRQEENRLRQENNPYRR-FLIEKAKEDFLKPMNNNMVLVFQQLYHKAREMVPVKNKLLLKDMHFKGFPLSILREASKGTRYEMFTRYMLHSNIPNAYLFSEPTPEKCRYAINITKKAHFLLLLGGIVDYRIMTVQQLNEFAALSSQGLDGARSHLVMLLEQNRGEQIVQDLNYHQSFIVNGLKNLSDEMK 247
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Match: A0A183LD84 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS1759 PE=4 SV=1)

HSP 1 Score: 124.405 bits (311), Expect = 8.373e-28
Identity = 77/229 (33.62%), Postives = 124/229 (54.15%), Query Frame = 2 
Query:   89 QNVRHRFNEDPGTLEQRLFRAVTEAVVP---FKPRDMLINSSXXXXXXXXXXXXXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNS--LQEAHANLVRNLSSCQ-ADMATNLQFHQISLISGLDELASRNK 757
            Q++R R+  DPGTLEQRLFR VT+AV+P     P +  +   +  +   +       FL+E+ +ED ++     M+LVF    HK+  +  +K+ LF   M F  +P  I++ A+KGT        +    + N  +  + + E  R A+   +KV  LLL+GGI+DYRI TV Q+++F AL+S  L  A ++LV  L   +   +  +L +HQ  +++GL +L    K
Sbjct:   20 QSIRFRYVPDPGTLEQRLFREVTKAVIPPINVNPVEQRLRRQEENRLRQENNPYRN-FLIERAKEDFLKPMNNNMVLVFQQLCHKAREMVPVKNKLFLKDMHFKGFPLSILREASKGTRYEMFTRYMLHSNIPNTYLFSEPTPEKCRYAINITKKVHFLLLLGGIVDYRIMTVQQLNEFAALSSQGLDGARSHLVMLLEQNRGGQILQDLNYHQSFIVNGLKKLYDETK 247
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Match: A0A183RA95 (Uncharacterized protein OS=Schistosoma rodhaini OX=6188 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.240e-27
Identity = 79/234 (33.76%), Postives = 124/234 (52.99%), Query Frame = 2 
Query:   89 QNVRHRFNEDPGTLEQRLFRAVTEAVVPFKPRDMLINSSXXXXXXXXXXX--------XXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNS--LQEAHANLVRNLSSCQAD-MATNLQFHQISLISGLDELASRNK 757
            Q++R R+  DPGTLEQRLFR VT+ V+P      LIN    ++   +            Y  FL+EK +ED ++     M+LVF   YHK+  +  +K+ L    M F  +P  I++ A+KGT        +    + N  +  + + E  R A+   +K   LLL+GGI+DYRI TV Q+++F AL+S  L  A ++LV  L   + + +  +L +HQ  +++GL  L+   K
Sbjct:   20 QSIRFRYVPDPGTLEQRLFREVTKPVIP------LINVHPVERRLLRQEENRLRQENNPYRRFLIEKAKEDFLKPMNNNMVLVFQQLYHKAREVVPVKNKLLLKDMHFKGFPLSILREASKGTRYEMFTRYMLHSNIPNAYLFSEPTPEKCRYAINITKKAHFLLLLGGIVDYRIMTVQQLNEFAALSSQGLDGARSHLVMLLEQNRGEKIVQDLNYHQSFIVNGLKNLSDEMK 247
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Match: G4VF29 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 GN=Smp_085780 PE=4 SV=1)

HSP 1 Score: 123.635 bits (309), Expect = 1.364e-27
Identity = 76/229 (33.19%), Postives = 122/229 (53.28%), Query Frame = 2 
Query:   89 QNVRHRFNEDPGTLEQRLFRAVTEAVVPF---KPRDMLINSSXXXXXXXXXXXXXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNS--LQEAHANLVRNLSSCQAD-MATNLQFHQISLISGLDELASRNK 757
            Q+VR R+  DPGTLEQRLFR VT+ V+P     P +  +   +  +   +       FL+EK +ED ++     M+LVF   YHK+  +  +K+ L    M F  +P  I++ A+KGT        +    + N  +  + + E  R A+   +K   LLL+GGI+DYRI TV Q+++F  L+S  L  A ++LV  L   + + +  +L +HQ  +++GL  L+   K
Sbjct:   20 QSVRFRYVPDPGTLEQRLFREVTKPVIPLINVHPVEQRLRRQEENRLRQENNPYRS-FLIEKAKEDFLKPMNNNMVLVFQQLYHKAREMVPVKNKLLLKDMHFKGFPLSILREASKGTRYEMFTRYMLHSNIPNAYLFSEPTPEKCRYAINITKKAHFLLLLGGIVDYRIMTVQQLNEFATLSSQGLDGARSHLVMLLEQNRGEQIVQDLNYHQSFIVNGLKNLSDEMK 247
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Match: SMESG000026635.1 (SMESG000026635.1)

HSP 1 Score: 783.482 bits (2022), Expect = 0.000e+0
Identity = 406/415 (97.83%), Postives = 409/415 (98.55%), Query Frame = 2 
Query: 1709 MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGKPNQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSLXXXXXXXXXXXXXMFASEHVDNILDDRVDINIDTKVNEVKDDERKNKLEITEPQNENKDPYEIMSNVDVSVPNGMPQKSEIEDLVMNKTDDKISLANEATLELQAAESNPIENLSEFQDSTTVNVSNQVFDNTDAYENENVFE--ERKFNEHVESKDLEDLEKLNQVEVKEMGDETDRDVNEILPQDSADDNQNNPDTVSDELYSDAAIKIQATYRGFRVRNELDKAKKSDFPEDSIDENPDLSEATNYEENI 2947
            MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGK NQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSLKKEDNKKKQNNKKMFASEHVDNILDDRVDINIDT+VNEVKDDERK+KLEITEPQNENKDPYEIMS+VDVSV NGMPQKSEIEDLVMNKTDDKISLANEATLELQAAESNPIENLSEFQDSTTVNVSNQVFDNTDAYENENVFE  ERKFN HVESKDLEDLEKLNQVEVKEMGDETDRDVNEILPQDSADDNQNNPDTVSDELYSDAA KIQATYRGFRVRNELDKAKKSDFPEDSIDENPDLSEATNYEENI
Sbjct:    1 MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGKLNQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSLKKEDNKKKQNNKKMFASEHVDNILDDRVDINIDTEVNEVKDDERKDKLEITEPQNENKDPYEIMSDVDVSVSNGMPQKSEIEDLVMNKTDDKISLANEATLELQAAESNPIENLSEFQDSTTVNVSNQVFDNTDAYENENVFEETERKFNGHVESKDLEDLEKLNQVEVKEMGDETDRDVNEILPQDSADDNQNNPDTVSDELYSDAATKIQATYRGFRVRNELDKAKKSDFPEDSIDENPDLSEATNYEENI 415
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Match: SMESG000026636.1 (SMESG000026636.1)

HSP 1 Score: 460.685 bits (1184), Expect = 2.044e-155
Identity = 242/244 (99.18%), Postives = 243/244 (99.59%), Query Frame = 2 
Query:   26 MNIFKCFKNTSLFNKTLFYAIQNVRHRFNEDPGTLEQRLFRAVTEAVVPFKPRDMLINSSXXXXXXXXXXXXXXXFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNSLQEAHANLVRNLSSCQADMATNLQFHQISLISGLDELASRNK 757
            MNIFKCFKNTSLFNKTL YAIQNVRHRFNEDPG+LEQRLFRAVTEAVVPFKPRDMLINSSDYKKNDGKGYGNYGDFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNSLQEAHANLVRNLSSCQADMATNLQFHQISLISGLDELASRNK
Sbjct:    1 MNIFKCFKNTSLFNKTLSYAIQNVRHRFNEDPGSLEQRLFRAVTEAVVPFKPRDMLINSSDYKKNDGKGYGNYGDFLVEKCREDVIESAKGRMLLVFHMNYHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKVLNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIALNSLQEAHANLVRNLSSCQADMATNLQFHQISLISGLDELASRNK 244
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Match: SMESG000033740.1 (SMESG000033740.1)

HSP 1 Score: 142.895 bits (359), Expect = 2.713e-34
Identity = 70/162 (43.21%), Postives = 99/162 (61.11%), Query Frame = 2 
Query: 1709 MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGKPNQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSL 2194
            M+SRY+ D++ R+SKIIH+YRNGD Y RA  V+LR  EQ  LERI KAIEEK+ LP N+PI+++F + G  +     I D  YYV +S ENF++ +YG    W ++GPV+ SK+ +V Y    +E+     LS  N V+   R   P   S +   K  +S+
Sbjct:    1 MTSRYMIDIENRKSKIIHVYRNGDKYNRAALVLLRPMEQQKLERIFKAIEEKLTLPYNKPIKRIFTIKGKEIDDPIMIKDQKYYVVSSLENFINTRYGSIIKWAIIGPVKWSKFADVFYEPPEQED-----LSTGNKVI--YRWKNPTQSSARFPSKMTKSM 155
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Match: SMESG000033740.1 (SMESG000033740.1)

HSP 1 Score: 142.895 bits (359), Expect = 3.081e-34
Identity = 70/162 (43.21%), Postives = 99/162 (61.11%), Query Frame = 2 
Query: 1709 MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGKPNQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSL 2194
            M+SRY+ D++ R+SKIIH+YRNGD Y RA  V+LR  EQ  LERI KAIEEK+ LP N+PI+++F + G  +     I D  YYV +S ENF++ +YG    W ++GPV+ SK+ +V Y    +E+     LS  N V+   R   P   S +   K  +S+
Sbjct:    1 MTSRYMIDIENRKSKIIHVYRNGDKYNRAALVLLRPMEQQKLERIFKAIEEKLTLPYNKPIKRIFTIKGKEIDDPIMIKDQKYYVVSSLENFINTRYGSIIKWAIIGPVKWSKFADVFYEPPEQED-----LSTGNKVI--YRWKNPTQSSARFPSKMTKSM 155
[Close]
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Match: SMESG000033740.1 (SMESG000033740.1)

HSP 1 Score: 133.65 bits (335), Expect = 2.212e-31
Identity = 66/155 (42.58%), Postives = 93/155 (60.00%), Query Frame = 2 
Query: 1730 DLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIEEKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHPANWVLVGPVRTSKYENVHYVTRAEEEGKPNQLSASNNVLSTSRTNKPVTPSKKSGRKSIQSL 2194
            D++ R+SKIIH+YRNGD Y RA  V+LR  EQ  LERI KAIEEK+ LP N+PI+++F + G  +     I D  YYV +S ENF++ +YG    W ++GPV+ SK+ +V Y    +E+     LS  N V+   R   P   S +   K  +S+
Sbjct:    3 DIENRKSKIIHVYRNGDKYNRAALVLLRPMEQQKLERIFKAIEEKLTLPYNKPIKRIFTIKGKEIDDPIMIKDQKYYVVSSLENFINTRYGSIIKWAIIGPVKWSKFADVFYEPPEQED-----LSTGNKVI--YRWKNPTQSSARFPSKMTKSM 150
The following BLAST results are available for this feature:
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 1
Match NameE-valueIdentityDescription
sp|Q550F6|DCX_DICDI2.060e-630.39Protein doublecortin OS=Dictyostelium discoideum O... [more]
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A094ZYL22.893e-2833.6239S ribosomal protein L10, mitochondrial OS=Schist... [more]
A0A3Q0KIQ73.504e-2833.62Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
A0A183LD848.373e-2833.62Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A183RA951.240e-2733.76Uncharacterized protein OS=Schistosoma rodhaini OX... [more]
G4VF291.364e-2733.19Uncharacterized protein OS=Schistosoma mansoni OX=... [more]
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of 39S ribosomal protein L10, mitochondrial vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000026635.10.000e+097.83SMESG000026635.1[more]
SMESG000026636.12.044e-15599.18SMESG000026636.1[more]
SMESG000033740.12.713e-3443.21SMESG000033740.1[more]
SMESG000033740.13.081e-3443.21SMESG000033740.1[more]
SMESG000033740.12.212e-3142.58SMESG000033740.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010905 ID=SMED30010905|Name=39S ribosomal protein L10, mitochondrial|organism=Schmidtea mediterranea sexual|type=transcript|length=3172bp
ATCTATATAAATCTTATATGATCAAATGAATATTTTCAAGTGCTTTAAAA
ATACTTCTCTCTTTAATAAAACTTTATTCTATGCAATACAAAATGTTCGT
CATAGATTTAATGAAGACCCTGGTACCTTAGAGCAAAGATTGTTTCGTGC
TGTAACAGAAGCTGTCGTTCCTTTTAAACCCAGAGACATGCTAATCAACA
GTTCTGATTATAAAAAAAATGACGGAAAAGGTTACGGAAATTATGGTGAT
TTTTTAGTAGAAAAATGTCGAGAGGATGTAATTGAGTCAGCTAAAGGAAG
AATGTTATTGGTGTTCCATATGAATTATCATAAAAGTTACCACTTATTTA
ACTTGAAGAGCAGTTTGTTTGATAATGGGATGACATTTCATTTTTATCCC
AGTGAGATTATGAAGGCGGCTGCAAAGGGTACAAATCTATCGGATGTGGT
CAACACGCTATTCCTGGACAAGGTACTGAACATGGTGGTTATTGGTGATT
GCTCATCTGAGTTGGCTCGAGTAGCAGTTGAATCCGGTAGGAAAGTAAAG
CAACTGCTTTTAATTGGCGGAATTCTTGACTATCGGATCGCAACTGTCGA
TCAGATCGATCAGTTCATCGCGCTCAACAGCCTGCAGGAGGCACATGCGA
ATCTCGTCAGGAATTTATCCTCCTGCCAAGCCGACATGGCTACCAATCTG
CAATTCCACCAGATCAGTTTGATTTCCGGTCTCGACGAGCTGGCCAGTCG
GAATAAATAAATATTTTGTTGACTTTCTTTTGTTTGAATGGCTGTGAGCT
GGTTGGAGTTTTGTTTTGATTGCTGATGAGATCTTGCTAGTTGATTGCCA
TTCATATTTAGGAATTTGACATTCATTTGTGAAATGGGACAGTTTGGCAC
ATTTACATCAACCGTACCACCTCAATTTATTTATGTTTCCTAGTAGTTAC
ATTCTGCAAACAGTTTCAGGAGGCCTTCATAACCGAGGTGTATTTTTATA
TATGTTCGTTAATACTCAGGATTTTTTTTAATTGTAGCGTATTTTAGCTT
CCCTGACGCAATTTCTTAGACTGTTCTGCTATAATTCATTCCCTCTTTGT
AATATTAATAATAAATTATTCAATGTAATATATGACATCACAGTTCCATC
CTTCCATGTGCTCGAGTAGAATTTTAGGAGTTAGAGAAATTAAGTTGTAT
TTTTGAGTTATTTTATCACCAATAAAATTGGCTTAGTAAAAGGCAAAAAT
AATCCTGATTTATTTTACAGCGATTTATAAATATTTTTCACCCAGGTCCT
GTGCTGAATTAATCCATCGATTTCTGCAACAATATATAATTTCCTTCAAA
TGAATCGCGATTGGGTAGTGGATATTAAAAAGTTGTCAACCTTTATAAAC
AAAATTTTATATATGAACGGATGGCCGCCGTATTTTCATGGATAGTAACT
CCATTTCCAGGAAAATAATCTATGAATAACCATAAAGCTGCTCTGTGAAA
AAGTCCAATAATTGAAATGTAAATATTTCTCCCTGAACTGACTGGCAACT
CCTAACAACAACCATTGAAAATCAATCCGTAAGTTGTGCTGAGCAATATT
TCATTGAGTTGTGCAATCATTGATCGATATGAGCAGAGAAAATAAATATT
GATTTATGTTAGAAATTATTTGCAAATTTCCCATTCAGTTGATTTCTTTT
CACCATAAATGAGCTCTCGGTATTTGAAGGATCTCGATATTAGGCGATCT
AAAATTATCCATATCTATCGGAACGGTGATCCGTATGGTCGGGCGATTTT
TGTCATTCTCAGACTTCCTGAACAGGCGGCTTTAGAGAGGATTGTTAAAG
CTATCGAAGAGAAAGTCAGATTACCGCTCAATAGGCCAATACGAAAATTA
TTTGAATTAACCGGGACACTTGTTCAGAGTCATACTCAAATTGTGGATGG
CAATTATTATGTTGCTGCTTCTTCAGAAAATTTTCTCGATATTAAATATG
GCCATCCTGCCAATTGGGTCTTGGTAGGCCCTGTTAGAACCAGCAAGTAC
GAGAATGTCCACTACGTCACCAGAGCAGAAGAAGAGGGTAAGCCTAATCA
GTTATCGGCGTCCAATAATGTTTTATCCACATCAAGAACAAACAAACCAG
TGACTCCATCAAAAAAATCCGGAAGAAAGAGTATTCAATCTCTTAAGAAA
GAAGATAATAAAAAAAAACAAAATAATAAAAAGATGTTTGCATCTGAACA
TGTCGACAATATCCTTGATGATCGTGTGGACATTAACATTGACACTAAGG
TCAACGAAGTGAAGGATGATGAAAGAAAGAATAAATTAGAAATCACTGAA
CCTCAAAATGAAAACAAAGATCCGTATGAAATAATGTCTAATGTAGACGT
ATCAGTTCCCAACGGAATGCCACAGAAAAGCGAGATCGAGGATTTGGTAA
TGAATAAAACTGACGATAAGATCTCTTTAGCCAATGAAGCGACTCTAGAA
TTGCAAGCTGCAGAATCAAATCCGATTGAAAATTTATCAGAATTTCAAGA
CTCCACAACTGTCAACGTATCAAATCAGGTTTTCGATAACACTGATGCGT
ATGAGAATGAAAATGTGTTTGAAGAAAGGAAATTCAATGAGCATGTCGAA
TCTAAAGATTTAGAAGATCTTGAAAAATTGAATCAAGTTGAAGTCAAGGA
GATGGGAGATGAAACCGACCGAGATGTCAATGAAATACTCCCACAAGACT
CGGCTGATGACAATCAGAATAATCCGGATACAGTTTCAGATGAACTTTAT
TCCGATGCTGCAATCAAAATTCAAGCGACATATCGAGGATTTCGTGTAAG
AAACGAATTGGATAAAGCAAAAAAGAGCGATTTTCCTGAAGATAGTATCG
ACGAAAATCCCGACCTAAGTGAAGCCACCAATTATGAAGAAAATATTTAG
ATTTTTTCGTATAAATGTTTGATCCCTTGAATTATTGCTGCTATTCATTC
TTAGATATTTTCTTTCTCTCACAAATTTTAGTTATTTTAAAATTTCTTTG
AGTTTCGAATGTTTTAATTTTCTTTACTTTCATTTATATCATTTGTATGA
TTCTATGTGACATGCTAAACGTTTATGCGGATGTGATTTCACTATTTAAT
ATATCTATTATTAGCAAAAAAA
back to top

protein sequence of SMED30010905-orf-1

>SMED30010905-orf-1 ID=SMED30010905-orf-1|Name=SMED30010905-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=245bp
MNIFKCFKNTSLFNKTLFYAIQNVRHRFNEDPGTLEQRLFRAVTEAVVPF
KPRDMLINSSDYKKNDGKGYGNYGDFLVEKCREDVIESAKGRMLLVFHMN
YHKSYHLFNLKSSLFDNGMTFHFYPSEIMKAAAKGTNLSDVVNTLFLDKV
LNMVVIGDCSSELARVAVESGRKVKQLLLIGGILDYRIATVDQIDQFIAL
NSLQEAHANLVRNLSSCQADMATNLQFHQISLISGLDELASRNK*
back to top

protein sequence of SMED30010905-orf-2

>SMED30010905-orf-2 ID=SMED30010905-orf-2|Name=SMED30010905-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=414bp
MSSRYLKDLDIRRSKIIHIYRNGDPYGRAIFVILRLPEQAALERIVKAIE
EKVRLPLNRPIRKLFELTGTLVQSHTQIVDGNYYVAASSENFLDIKYGHP
ANWVLVGPVRTSKYENVHYVTRAEEEGKPNQLSASNNVLSTSRTNKPVTP
SKKSGRKSIQSLKKEDNKKKQNNKKMFASEHVDNILDDRVDINIDTKVNE
VKDDERKNKLEITEPQNENKDPYEIMSNVDVSVPNGMPQKSEIEDLVMNK
TDDKISLANEATLELQAAESNPIENLSEFQDSTTVNVSNQVFDNTDAYEN
ENVFEERKFNEHVESKDLEDLEKLNQVEVKEMGDETDRDVNEILPQDSAD
DNQNNPDTVSDELYSDAAIKIQATYRGFRVRNELDKAKKSDFPEDSIDEN
PDLSEATNYEENI*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0042254ribosome biogenesis
GO:0035556intracellular signal transduction
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000418head
PLANA:0003117parapharyngeal cell
Vocabulary: INTERPRO
TermDefinition
IPR036572Doublecortin_dom_sf
IPR003533Doublecortin_dom
IPR000048IQ_motif_EF-hand-BS
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003533Doublecortin domainSMARTSM00537doub1_11coord: 10..98
e-value: 2.1E-5
score: 33.9
IPR003533Doublecortin domainPFAMPF03607DCXcoord: 43..92
e-value: 2.4E-8
score: 33.9
IPR003533Doublecortin domainPROSITEPS50309DCcoord: 15..98
score: 18.168
NoneNo IPR availableGENE3DG3DSA:1.20.5.190coord: 354..392
e-value: 3.9E-5
score: 25.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 158..180
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 131..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 386..415
NoneNo IPR availablePANTHERPTHR23004DOUBLECORTIN DOMAIN CONTAINING 2coord: 12..233
NoneNo IPR availableCDDcd16113DCX2_DCDC2_likecoord: 14..88
e-value: 8.71977E-14
score: 64.1346
IPR000048IQ motif, EF-hand binding sitePFAMPF00612IQcoord: 368..385
e-value: 1.9E-4
score: 20.9
IPR000048IQ motif, EF-hand binding sitePROSITEPS50096IQcoord: 366..395
score: 9.285
IPR036572Doublecortin domain superfamilyGENE3DG3DSA:3.10.20.230coord: 14..100
e-value: 7.0E-16
score: 59.6
IPR036572Doublecortin domain superfamilySUPERFAMILYSSF89837Doublecortin (DC)coord: 5..98