Eukaryotic initiation factor-4G

Overview
NameEukaryotic initiation factor-4G
Smed IDSMED30010857
Length (bp)1308
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Eukaryotic initiation factor-4G (SMED30010857) t-SNE clustered cells

Violin plots show distribution of expression levels for Eukaryotic initiation factor-4G (SMED30010857) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Eukaryotic initiation factor-4G (SMED30010857) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Eukaryotic initiation factor-4G (SMED30010857) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010857

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010857SMESG000045255.1 SmedASXL_011843SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30010857SMESG000045255.1 dd_Smed_v4_10036_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010857SMESG000045255.1 Contig14671newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010857SMESG000045255.1 Contig14671uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010857SMESG000045255.1 Contig21759newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010857SMESG000045255.1 Contig21759uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2])

HSP 1 Score: 50.447 bits (119), Expect = 4.531e-6
Identity = 25/80 (31.25%), Postives = 42/80 (52.50%), Query Frame = 2 
Query:  533 FERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNI-SFFKDLFKDV 769
             ER  + R  +G  ++ LI+ G+L   QL +G S  +E  DD+  D+P  W +LA+ L   ++    N+   F +L K +
Sbjct: 1393 LERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPL 1472
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2])

HSP 1 Score: 49.2914 bits (116), Expect = 8.572e-6
Identity = 25/78 (32.05%), Postives = 41/78 (52.56%), Query Frame = 2 
Query:  533 FERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNI-SFFKDLFK 763
             ER  + R  +G  ++ LI+ G+L   QL +G S  +E  DD+  D+P  W +LA+ L   ++    N+   F +L K
Sbjct:  998 LERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSK 1075
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Match: eif4g3a (eukaryotic translation initiation factor 4 gamma, 3a [Source:ZFIN;Acc:ZDB-GENE-120810-2])

HSP 1 Score: 49.2914 bits (116), Expect = 9.676e-6
Identity = 25/78 (32.05%), Postives = 41/78 (52.56%), Query Frame = 2 
Query:  533 FERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNI-SFFKDLFK 763
             ER  + R  +G  ++ LI+ G+L   QL +G S  +E  DD+  D+P  W +LA+ L   ++    N+   F +L K
Sbjct: 1001 LERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSK 1078
[Close]
BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Match: D5JG54 (Eukaryotic initiation factor-4G OS=Dugesia japonica OX=6161 GN=eIF4G PE=2 SV=1)

HSP 1 Score: 111.694 bits (278), Expect = 9.955e-23
Identity = 83/278 (29.86%), Postives = 125/278 (44.96%), Query Frame = 2 
Query:  116 RNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQWNAGCQSKSNNSNGFSTIPSRKQVSYDDII---------DKPTVVVIKTQNKYAKLCEDFEVE--------LPSVPV-----KVNNDEALTTELFDLLMNNLAT-----VDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 868
            RN        +  K L  +   + E TVLGP+    W  G  SKS +      IP   +     I            P V++    NKY  L    E           P +P+     +V   E    E  D++ N+L +     ++ WK+L     V+EKW   M E+   CR  +GD F+ L+   ++T +Q+I G    + ++DD+I D+PK W F AQFLERFI  ++SNI FF+ +FK +   +  V  CF      +S R+   IV  +F +
Sbjct:  111 RNVNHAGNKNVSIKNLSCHRHTVDEKTVLGPA-GGSWIYGSGSKSKSHEAEVWIPVSLKTKGGTIRYTSNSRSREQSPQVIL---GNKYRNLESSAEASGADSRSSTPPMIPLISKAYRVVEPEMSDEERADIIFNDLLSTGSFDINSWKKLRTIPVVIEKWIDDMMEKDASCRRTIGDNFNRLLINKIITTEQIIDGTKSFVSSIDDLICDIPKLWNFFAQFLERFILDKNSNIKFFESIFKSILPNQD-VANCFSAALHDASDRLGHKIVGQVFHR 383
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287])

HSP 1 Score: 51.6026 bits (122), Expect = 1.401e-6
Identity = 34/109 (31.19%), Postives = 54/109 (49.54%), Query Frame = 2 
Query:  470 VDKWKELFNPVEVVEKWFAL-MF---------ERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKD 766
            ++ +KE    VE +E+   L MF         ER    R  +G  FH L++ G L+  Q  +G S T+E  DD+  D+P  W +LA+ +   +R     IS  ++LF +
Sbjct: 1387 INDYKEAVQCVEELEQASMLYMFVRVGVESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLR--EGGIS-MRELFSE 1492
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287])

HSP 1 Score: 51.6026 bits (122), Expect = 1.429e-6
Identity = 34/109 (31.19%), Postives = 54/109 (49.54%), Query Frame = 2 
Query:  470 VDKWKELFNPVEVVEKWFAL-MF---------ERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKD 766
            ++ +KE    VE +E+   L MF         ER    R  +G  FH L++ G L+  Q  +G S T+E  DD+  D+P  W +LA+ +   +R     IS  ++LF +
Sbjct: 1421 INDYKEAVQCVEELEQASMLYMFVRVGVESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLR--EGGIS-MRELFSE 1526
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287])

HSP 1 Score: 51.6026 bits (122), Expect = 1.461e-6
Identity = 34/109 (31.19%), Postives = 54/109 (49.54%), Query Frame = 2 
Query:  470 VDKWKELFNPVEVVEKWFAL-MF---------ERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKD 766
            ++ +KE    VE +E+   L MF         ER    R  +G  FH L++ G L+  Q  +G S T+E  DD+  D+P  W +LA+ +   +R     IS  ++LF +
Sbjct: 1362 INDYKEAVQCVEELEQASMLYMFVRVGVESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLR--EGGIS-MRELFSE 1467
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287])

HSP 1 Score: 51.6026 bits (122), Expect = 1.465e-6
Identity = 34/109 (31.19%), Postives = 54/109 (49.54%), Query Frame = 2 
Query:  470 VDKWKELFNPVEVVEKWFAL-MF---------ERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKD 766
            ++ +KE    VE +E+   L MF         ER    R  +G  FH L++ G L+  Q  +G S T+E  DD+  D+P  W +LA+ +   +R     IS  ++LF +
Sbjct: 1405 INDYKEAVQCVEELEQASMLYMFVRVGVESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLR--EGGIS-MRELFSE 1510
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Match: eif4g3b (eukaryotic translation initiation factor 4 gamma 3 [Source:NCBI gene;Acc:103031287])

HSP 1 Score: 51.6026 bits (122), Expect = 1.723e-6
Identity = 34/116 (29.31%), Postives = 57/116 (49.14%), Query Frame = 2 
Query:  449 LMNNLATVDKWKELFNPVEVVEKWFAL-MF---------ERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKD 766
            +++    ++ +KE    VE +E+   L MF         ER    R  +G  FH L++ G L+  Q  +G S T+E  DD+  D+P  W +LA+ +   +R     IS  ++LF +
Sbjct: 1253 IIDEFLHINDYKEAVQCVEELEQASMLYMFVRVGVESTLERSQITRDHMGQLFHQLLQAGSLSKPQFFKGFSETLELADDMAIDIPHIWLYLAELVNPVLR--EGGIS-MRELFSE 1365
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000045255.1 (SMESG000045255.1)

HSP 1 Score: 539.65 bits (1389), Expect = 1.627e-178
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 2 
Query:  113 MRNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQWNAGCQSKSNNSNGFSTIPSRKQVSYDDIIDKPTVVVIKTQNKYAKLCEDFEVELPSVPVKVNNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGKLEK 877
            MRNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQWNAGCQSKSNNSNGFSTIPSRKQVSYDDIIDKPTVVVIKTQNKYAKLCEDFEVELPSVPVKVNNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGKLEK
Sbjct: 1314 MRNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQWNAGCQSKSNNSNGFSTIPSRKQVSYDDIIDKPTVVVIKTQNKYAKLCEDFEVELPSVPVKVNNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGKLEK 1568
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000035786.1 (SMESG000035786.1)

HSP 1 Score: 156.377 bits (394), Expect = 4.005e-41
Identity = 92/255 (36.08%), Postives = 141/255 (55.29%), Query Frame = 2 
Query:  164 LVNPQNLSENTVLGPSLRTQWNAGCQSKSNNS------NGFSTIPSR--KQVSYDDIIDKPTVVVIKTQNKYAKLCEDFE-VELPSV----PVKV-------NNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 868
              N Q  + N    P ++ Q + G    SN         G++TI  +  KQ   + +        +KTQNK++ L E+ E  E+P      PVKV        NDE ++ ELF  +M + A+ D+W+++  PVE+++KW   + ER+ + R  +GDYF+ L +  LL+ D  I+G+   +E   D+I D+PK WEF A+FLERFI +  SNI FF   FK+VE + S + KC    + ++S R+ SD+VE+LF K
Sbjct:  558 FTNNQKGATNNSYKPDIKVQKSTGNTKPSNEKVPVQKIGGWNTIVKKTPKQDFVNKLAGADKSKALKTQNKFSLLAEEKETAEIPVCSIDKPVKVLTTISDNRNDEEISEELFKDIMKSEASPDRWRQISKPVEIIDKWLDKILERRSDSRALLGDYFNKLFKSKLLSKDDFIEGSKQILECSGDLISDIPKLWEFWAEFLERFIENGESNIGFFNLFFKNVENK-SDITKCRNCIKAYASERVGSDVVESLFAK 811
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000040405.1 (SMESG000040405.1)

HSP 1 Score: 148.673 bits (374), Expect = 1.505e-38
Identity = 91/254 (35.83%), Postives = 139/254 (54.72%), Query Frame = 2 
Query:  167 VNPQNLSENTVLGPSLRTQWNAGCQSKSNNS------NGFSTIPSR--KQVSYDDIIDKPTVVVIKTQNKYAKLCEDFE-VELPSV----PVKV-------NNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 868
             N Q  + N    P ++ Q + G    SN         G++TI  +  KQ   + +        +KTQNK++ L E+ E  E+P      PVKV        NDE ++ ELF  +M + A+ D+W ++  PVE+++KW   + ER+ + R  +GDYF++L +  LL  D  I+G+   +E  + +I D+PK WEF A+FLERFI +  SNI FF   FK+VE + S + KC    +  +S R+ SD+VE+LF K
Sbjct:  547 TNNQKGATNNSYKPDIKVQKSTGNTKPSNEKVPVQKIGGWNTIVKKTPKQDFVNKLAGADKSKALKTQNKFSLLAEEKETAEIPVCSIDKPVKVLTTISDNRNDEEISEELFKDIMKSEASPDRWGQISKPVEIIDKWLDKILERRSDSRALLGDYFNNLFKLKLLNKDDFIEGSKQILECSEYMISDIPKLWEFWAEFLERFIENGESNIGFFNLFFKNVENK-SDIIKCRNCIKALASERVGSDVVESLFAK 799
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000005574.1 (SMESG000005574.1)

HSP 1 Score: 143.665 bits (361), Expect = 7.399e-37
Identity = 91/255 (35.69%), Postives = 137/255 (53.73%), Query Frame = 2 
Query:  164 LVNPQNLSENTVLGPSLRTQWNAGCQSKSNNS------NGFSTIPSR--KQVSYDDIIDKPTVVVIKTQNKYAKLCEDFEVELPSV-----PVKV-------NNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 868
              N Q    N    P ++ Q + G    SN         G++TI  +  KQ   + +        +KTQNK++ L E+ E    SV     PVKV        NDE ++ ELF  +M + A+ D+W ++  PVE+++KW   + ER+ + R  +GDYF+ L +  LL+ D  I+G+   +E   D+I D+PK WEF A+FLERFI +  SNI FF   FK+V+ + S + KC    +  +S R+ SD+VE+LF K
Sbjct:  546 FTNNQKGVTNNSYKPDIKVQKSTGNTKPSNEKVPVRKIGGWNTIVKKTPKQDFVNKLAGADKSKALKTQNKFSLLAEEKESAEISVCSIDKPVKVLTTISDNRNDEEISEELFKDIMKSEASSDRWHQISKPVEIIDKWLDKILERRSDSRALLGDYFNKLFKLKLLSKDDFIEGSKQILECSGDMISDIPKLWEFWAEFLERFIENGESNIGFFNQFFKNVKNK-SDITKCRNCIKALASERVGSDVVESLFAK 799
[Close]
BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Match: SMESG000040439.1 (SMESG000040439.1)

HSP 1 Score: 142.895 bits (359), Expect = 1.409e-36
Identity = 90/255 (35.29%), Postives = 139/255 (54.51%), Query Frame = 2 
Query:  164 LVNPQNLSENTVLGPSLRTQWNAGCQSKSNNS------NGFSTIPSR--KQVSYDDIIDKPTVVVIKTQNKYAKLCEDFE-VELPSV----PVKV-------NNDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMRVGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLERFIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALFGK 868
              N Q  + N    P ++ Q   G    SN         G++TI  +  KQ   + +        +KTQNK++ L E+ E  E+P      PVKV        NDE ++ ELF  +M + A+ D+W ++  PVE+++KW   + ER+ + R  +GDYF+ L +  LL+ D  I+G+   +E   D+I D+PK WEF A+FLERFI +  SNI FF   FK+V+ + S + KC    + ++S R+ SD++E+LF K
Sbjct:  558 FTNNQKGATNNSYKPDIKVQKPTGNTKHSNEKVPVQKIGGWNTIVKKTPKQDFVNKLAGADKSKALKTQNKFSLLAEEKETAEIPVCSIDKPVKVLTTISDNRNDEEISEELFKDIMKSEASPDRWGQISKPVEIIDKWLDKILERRSDSRALLGDYFNKLFKLKLLSKDDFIEGSKQILECSGDLISDIPKLWEFWAEFLERFIENGESNIGFFNLFFKNVKNK-SDITKCRNCIKAYASERVGSDVLESLFAK 811
The following BLAST results are available for this feature:
BLAST of Eukaryotic initiation factor-4G vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
eif4g3a4.531e-631.25eukaryotic translation initiation factor 4 gamma, ... [more]
eif4g3a8.572e-632.05eukaryotic translation initiation factor 4 gamma, ... [more]
eif4g3a9.676e-632.05eukaryotic translation initiation factor 4 gamma, ... [more]
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
D5JG549.955e-2329.86Eukaryotic initiation factor-4G OS=Dugesia japonic... [more]
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
eif4g3b1.401e-631.19eukaryotic translation initiation factor 4 gamma 3... [more]
eif4g3b1.429e-631.19eukaryotic translation initiation factor 4 gamma 3... [more]
eif4g3b1.461e-631.19eukaryotic translation initiation factor 4 gamma 3... [more]
eif4g3b1.465e-631.19eukaryotic translation initiation factor 4 gamma 3... [more]
eif4g3b1.723e-629.31eukaryotic translation initiation factor 4 gamma 3... [more]
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Eukaryotic initiation factor-4G vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000045255.11.627e-178100.00SMESG000045255.1[more]
SMESG000035786.14.005e-4136.08SMESG000035786.1[more]
SMESG000040405.11.505e-3835.83SMESG000040405.1[more]
SMESG000005574.17.399e-3735.69SMESG000005574.1[more]
SMESG000040439.11.409e-3635.29SMESG000040439.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010857 ID=SMED30010857|Name=Eukaryotic initiation factor-4G|organism=Schmidtea mediterranea sexual|type=transcript|length=1308bp
AATCACTTTGCACAGTATAGCATATGATTTTTTAACTCATGATTATATAT
TTATATTTATTTAAAACGGAGTTTACGTTAATAATAATAATAATAATACT
AACAACAATAACATGAGAAACCGTCAGCCATATTCTCGTCCAGTAATGGA
CACGAAGAAACTGTTAGTAAATCCTCAAAACCTATCAGAAAATACAGTGC
TAGGACCTTCATTACGAACCCAGTGGAATGCGGGTTGCCAATCGAAATCA
AATAATAGCAACGGATTTTCCACAATACCATCACGTAAGCAAGTCTCTTA
CGATGACATAATCGACAAACCAACCGTCGTCGTCATTAAAACCCAAAATA
AATACGCAAAATTGTGCGAAGATTTCGAAGTTGAACTACCAAGCGTTCCA
GTTAAAGTCAACAATGATGAAGCTCTCACAACGGAATTGTTCGATTTGCT
CATGAACAACCTAGCAACGGTGGATAAATGGAAAGAACTATTCAATCCGG
TTGAAGTCGTTGAAAAGTGGTTTGCGCTTATGTTCGAAAGAAAGGTCGAA
TGTCGAATGAGAGTCGGAGATTATTTCCACGATCTCATTGAAGGAGGCCT
GTTGACATTCGATCAATTGATTCAAGGAGCATCTGTAACCATGGAGACAG
TGGACGACATAATCGATGACGTGCCGAAATTCTGGGAATTTCTAGCTCAA
TTTCTTGAAAGATTCATCAGAAGTGAACACTCAAACATATCATTTTTCAA
AGATCTATTCAAAGATGTCGAAGGACAGGAATCGGGTGTTGAAAAGTGTT
TCAAATTCACCCAGCAGTTCTCATCAAGCAGAATCGACAGTGATATCGTG
GAAGCTTTATTCGGAAAGCTGGAAAAATAATAATAATCAATAATATTGCA
AACTTTTCACATCAATAAATCAATAGTATTGATTTTTGCTTTATTTTGCT
AATATAATAATCGATGTAAATAATTGTAGTGATTATGTAATACATCTCGG
TGATGTCATTTGTAGATTTGATGATATTGATTATTAACATTTATTATTGA
AGTAAATTTATATCAATTTCTCTCTCTCTCGCTCAGTGTGTGTTCATTGA
TGTTTTTTATCGATTGTCTAGAAATAATTTTACATGGATTCCGTTGTGAT
TTCGGTTACGTCATAACTAATGTAAATAGAATAAATTATGTCGGATGGAA
ATGTTTATTGTTGGGAATTTTTCTGTAATTATTGAGATAGATGGTTGCTG
ATTGGATGGTTTTTGTAAATTCTGATACTGTAATATAATTTATTATTGAC
TTTTTGGC
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protein sequence of SMED30010857-orf-1

>SMED30010857-orf-1 ID=SMED30010857-orf-1|Name=SMED30010857-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=256bp
MRNRQPYSRPVMDTKKLLVNPQNLSENTVLGPSLRTQWNAGCQSKSNNSN
GFSTIPSRKQVSYDDIIDKPTVVVIKTQNKYAKLCEDFEVELPSVPVKVN
NDEALTTELFDLLMNNLATVDKWKELFNPVEVVEKWFALMFERKVECRMR
VGDYFHDLIEGGLLTFDQLIQGASVTMETVDDIIDDVPKFWEFLAQFLER
FIRSEHSNISFFKDLFKDVEGQESGVEKCFKFTQQFSSSRIDSDIVEALF
GKLEK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0002109X1 cell
Vocabulary: molecular function
TermDefinition
GO:0005488binding
GO:0003743translation initiation factor activity
Vocabulary: INTERPRO
TermDefinition
IPR016021MIF4-like_sf
IPR003891Initiation_fac_eIF4g_MI
IPR016024ARM-type_fold
Vocabulary: biological process
TermDefinition
GO:0006413translational initiation
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016021MIF4G-like domain superfamilyGENE3DG3DSA:1.25.40.180coord: 126..262
e-value: 1.2E-18
score: 69.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..18
NoneNo IPR availablePANTHERPTHR23253EUKARYOTIC TRANSLATION INITIATION FACTOR 4 GAMMAcoord: 2..242
IPR003891Initiation factor eIF-4 gamma, MA3PROSITEPS51366MIcoord: 91..228
score: 11.291
IPR016024Armadillo-type foldSUPERFAMILYSSF48371ARM repeatcoord: 141..261