PiggyBac transposable element-derived protein 4
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010813 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Homology
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Match: CABZ01072083.2 (dual specificity phosphatase 26 [Source:NCBI gene;Acc:570815]) HSP 1 Score: 70.0922 bits (170), Expect = 1.389e-12 Identity = 34/105 (32.38%), Postives = 55/105 (52.38%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLL 927 V+Y + +S+ H D + ++ KP +I +YN+T+GGV+N DK Y+ R+ P I ML+ A N F++++ N W RRR+FL+ L Sbjct: 391 VSYHPRKNKNVLVMSTLHRDDAENSDEQSKPQMILDYNSTRGGVDNFDKLTSTYTCQRKTLHWPMVIFFNMLDVSAYNAFVLWMDLNPSWEAQKTNRRRIFLQDL 495
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Match: npffr2 (neuropeptide FF receptor 2 [Source:Xenbase;Acc:XB-GENE-994428]) HSP 1 Score: 67.781 bits (164), Expect = 8.306e-12 Identity = 44/131 (33.59%), Postives = 66/131 (50.38%), Query Frame = 1 Query: 544 DNANRTRFSCFLGRTGISRSDNCVTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD 936 D A R FS + R+G S + Y ++ C L+S H D +IG+ +KP I +YN+ K GV+ +D+ YS R P + ML+ A+N +I+Y GW +RR+FL LL K+ Sbjct: 205 DTAQREVFSTSVLRSG---SVSLTIYAPKKNLTVCVLNSMHQDVTIGDNRKRKPNTITDYNHMKCGVDVLDQMARMYSVRAATRRWPVAVYYNMLDLAAVNAYILY-KACTGWTG----KRRLFLSLLAKE 327
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: G3Q385 (DDE_Tnp_1_7 domain-containing protein OS=Gasterosteus aculeatus OX=69293 PE=4 SV=1) HSP 1 Score: 83.1889 bits (204), Expect = 4.521e-15 Identity = 39/115 (33.91%), Postives = 63/115 (54.78%), Query Frame = 1 Query: 589 GISRSDNCVTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVK 933 + V+Y +R +S+ H D+S+ KP +I +YN TKGGV+N+DK YS R+ AR P + +++ A N ++++I N WN + +RRR+FL+ L K Sbjct: 41 AFTEKTTLVSYCPKRNKNVLVMSTMHKDASLSTREDMKPQMILDYNATKGGVDNLDKVTATYSCQRKTARWPLVVFYNIVDVSAYNAYVLWIEINQQWNASKLYRRRLFLEELGK 155
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: G3N553 (DDE_Tnp_1_7 domain-containing protein OS=Gasterosteus aculeatus OX=69293 PE=4 SV=1) HSP 1 Score: 82.4185 bits (202), Expect = 8.775e-15 Identity = 39/107 (36.45%), Postives = 62/107 (57.94%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVK 933 V+Y +R +S+ H D+S+ KP +I +YN TKGGV+N+DK YS R+ AR P + +++ A N ++++I N WN + +RRR+FL+ L K Sbjct: 49 VSYCPKRNKYVLVMSTMHKDASLSTREDMKPQMILDYNATKGGVDNLDKVTATYSCQRKTARWPLVVFYNIVDVSAYNAYVLWIEINQQWNASKLYRRRLFLEELGK 155
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A0S7EQU4 (PGBD4 (Fragment) OS=Poeciliopsis prolifica OX=188132 GN=PGBD4 PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 1.390e-14 Identity = 42/130 (32.31%), Postives = 68/130 (52.31%), Query Frame = 1 Query: 589 GISRSDNCVTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILDVR 978 + V+Y +R +S+ H D+S+ KP +I +YN+TKGGV+N+DK YS R+ PF I +++ A N ++++I N WN + RRR+FL+ L K ++ R+ VR Sbjct: 13 AFTEKTTLVSYCPKRNKNVLVMSTMHKDASLSTREDMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTTYWPFVIFCNIVDVSAYNAYVLWIEINQQWNASKLHRRRLFLEELGKALVTPYIQNRVRPVR 142
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A1Y1LXG8 (DDE_Tnp_1_7 domain-containing protein (Fragment) OS=Photinus pyralis OX=7054 PE=4 SV=1) HSP 1 Score: 81.2629 bits (199), Expect = 1.899e-14 Identity = 43/128 (33.59%), Postives = 68/128 (53.12%), Query Frame = 1 Query: 589 GISRSDNCVTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILD 972 G V+Y + LSS+H DS+I +KP +I +YN TKG V+ +DK I EY+ R++ R P + M +++ A+N F++++ N W +RRMFL L K +++ RI D Sbjct: 10 GFQPEITMVSYVPKVNQSVILLSSEHRDSAISEGSKRKPEIITHYNATKGAVDTLDKMIAEYTCNRQSKRWPATLFMNIIDIAAVNAFVMWVHLNPEWERQYLDKRRMFLLELGKRLVQPHLQRRISD 137
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Match: A0A5C6MNA5 (DDE_Tnp_1_7 domain-containing protein OS=Takifugu flavidus OX=433684 GN=D4764_08G0002360 PE=4 SV=1) HSP 1 Score: 83.1889 bits (204), Expect = 2.596e-14 Identity = 41/109 (37.61%), Postives = 61/109 (55.96%), Query Frame = 1 Query: 607 NCVTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVK 933 + V+Y ++ +S+ H D I ++ H KP +I +YN TKGGV+N+DK + YS RR R P I ML+ A N F+I++ N W +RR+FL+ L K Sbjct: 106 SLVSYVPKKAKNVVLMSTLHGDGRICDQEHHKPEIIMDYNATKGGVDNIDKLVTAYSCKRRTLRWPLVIFFDMLDISAYNAFVIWMALNPEWKRVKLQKRRLFLEDLGK 214
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000037833.1 (piggyBac transposable element-derived protein 4-like [Source:NCBI gene;Acc:101160710]) HSP 1 Score: 82.4185 bits (202), Expect = 1.187e-16 Identity = 39/107 (36.45%), Postives = 62/107 (57.94%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVK 933 V+Y +R +S+ H+D+S+ KP +I +YN+TKGGV+N+DK YS R+ AR P I +++ A N ++++ N WN +RRR+FL+ L K Sbjct: 387 VSYCPKRNKNVLVMSTMHTDASLSTRDDMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTARWPMAIFFNIVDVSAYNAYVLWSEINQEWNAGKLYRRRLFLEELGK 493
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000027888.1 (pep primary_assembly:ASM223467v1:17:9217998:9219211:-1 gene:ENSORLG00000025878.1 transcript:ENSORLT00000027888.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 80.8777 bits (198), Expect = 1.788e-16 Identity = 38/107 (35.51%), Postives = 62/107 (57.94%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVK 933 V+Y +R +S+ H+D+S+ KP +I +YN+TKGGV+N+DK YS R+ AR P I +++ A N ++++ N WN +RR++FL+ L K Sbjct: 190 VSYCPKRNKNVLVMSTMHTDASLSTRDDMKPQMILDYNSTKGGVDNLDKVTATYSCQRKTARWPMAIFFNIVDVSAYNAYVLWSEINQEWNAGKLYRRQLFLEELSK 296
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Match: ENSORLT00000029842.1 (piggyBac transposable element-derived protein 4 [Source:NCBI gene;Acc:101157644]) HSP 1 Score: 67.0106 bits (162), Expect = 4.119e-13 Identity = 45/136 (33.09%), Postives = 72/136 (52.94%), Query Frame = 1 Query: 610 CVTYTKERKNGSCNLSSQHSDSSIGNE*H-QKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILDVRKKPSILKAIQAY 1014 ++Y ++ LS++HS I +E +KP +I +YN TKGGV+N+DK + YS R R P + M++ A N ++I+ + W RRR+FL+ L KD +R L +R+K + +A AY Sbjct: 637 ALSYVPKKNKNVLLLSTKHSRVEICHERRDKKPEMILDYNKTKGGVDNLDKLLATYSCRRMTKRWPLAMFHNMIDMSAYNAYVIWREMHPDWMPWNRNRRRIFLEQLGKD------LVRPLVLRRK-RVPRARPAY 765 HSP 2 Score: 26.5646 bits (57), Expect = 4.119e-13 Identity = 14/25 (56.00%), Postives = 17/25 (68.00%), Query Frame = 3 Query: 1035 VQTQEAAQTS-RATGK*GRCRLCPR 1106 V QEA TS R+ GK RC++CPR Sbjct: 769 VSMQEAGGTSARSEGKRKRCQICPR 793
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000015322.1 (SMESG000015322.1) HSP 1 Score: 135.961 bits (341), Expect = 1.588e-39 Identity = 67/70 (95.71%), Postives = 67/70 (95.71%), Query Frame = 1 Query: 817 MQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILDVRKKPSILKAIQAYGELL 1026 MQMLNTEALN FIIYITKNHGWNTNLGFRRRMFLKLLVKD TNQNMKLRILDVRK PSILKAIQAYGELL Sbjct: 1 MQMLNTEALNAFIIYITKNHGWNTNLGFRRRMFLKLLVKDLTNQNMKLRILDVRKTPSILKAIQAYGELL 70
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000015322.1 (SMESG000015322.1) HSP 1 Score: 131.339 bits (329), Expect = 7.840e-38 Identity = 65/68 (95.59%), Postives = 65/68 (95.59%), Query Frame = 1 Query: 823 MLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILDVRKKPSILKAIQAYGELL 1026 MLNTEALN FIIYITKNHGWNTNLGFRRRMFLKLLVKD TNQNMKLRILDVRK PSILKAIQAYGELL Sbjct: 1 MLNTEALNAFIIYITKNHGWNTNLGFRRRMFLKLLVKDLTNQNMKLRILDVRKTPSILKAIQAYGELL 68
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000070813.1 (SMESG000070813.1) HSP 1 Score: 65.4698 bits (158), Expect = 1.878e-11 Identity = 40/134 (29.85%), Postives = 70/134 (52.24%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSSIGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRILDVRKKPSILKAIQAY 1014 V+Y ++ L ++ ++ I E + KP +I YN TKG V++ D EYS R R FR+ M++++ A N ++++ + W N RR++FL+ L K +N++ R D+ K SI + I + Sbjct: 212 VSYIPKKGKAVNLLITKFHNNEICKEENNKPRIILYYNKTKGAVDSGDMMTFEYSIVRVTRRWAFRLFMELIDIAAFNAYVVWKAVHPDWRNNDSGRRKVFLRELAKALATKNVQDRKYDMFHK-SIKRCIDIF 344
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000081807.1 (SMESG000081807.1) HSP 1 Score: 60.8474 bits (146), Expect = 6.268e-10 Identity = 39/155 (25.16%), Postives = 73/155 (47.10%), Query Frame = 1 Query: 574 FLGRTGISRSDNCVTYTKERKNGSCNLSSQHSDSSIGNE*H-QKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFLKLLVKD*TNQNMKLRIL------DVRKKPSILKAIQAYG 1017 F + ++ V+Y + LS+ H+ + ++ +KP ++ +YN TKG V+ D+ + YS+A + + P R+ +++ LN F++ N W + G RR +L + + MK I+ + KPS+ +AI A G Sbjct: 250 FESKFAFTKDTALVSYAPPKNRTVVLLSTMHNRPVVFDDGRGRKPYMVLDYNMTKGAVDAFDQEVSFYSFAHKTRQWPMRLFYFIIDASCLNAFVLLNMNNPTWKDHEGSRRLDKRRLFLTKAAHSLMKPMIIARADNKSISHKPSVARAILAVG 404
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Match: SMESG000032747.1 (SMESG000032747.1) HSP 1 Score: 51.2174 bits (121), Expect = 8.800e-7 Identity = 31/104 (29.81%), Postives = 53/104 (50.96%), Query Frame = 1 Query: 613 VTYTKERKNGSCNLSSQHSDSS--IGNE*HQKPCVIENYNNTKGGVNNVDKFIGEYSYARRAAR*PFRILMQMLNTEALNTFIIYITKNHGWNTNLGFRRRMFL 918 V+Y ++ + LS+ H+ + +G++ KP V+ YN TKGGV+ +D+ + Y+ + R +L+ A N F ++ WN N RRR+FL Sbjct: 48 VSYVAKKSKVANILSTAHNKITGYVGSK--HKPDVLLFYNATKGGVDAMDERVATYTTKFKCRRWHVAFFCNILDISAFNAFALHYLVEPQWNANKSHRRRLFL 149 The following BLAST results are available for this feature:
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 1
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of PiggyBac transposable element-derived protein 4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 3
BLAST of PiggyBac transposable element-derived protein 4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010813 ID=SMED30010813|Name=PiggyBac transposable element-derived protein 4|organism=Schmidtea mediterranea sexual|type=transcript|length=1181bpback to top Annotated Terms
The following terms have been associated with this transcript:
|