SMED30010669

Overview
NameSMED30010669
Smed IDSMED30010669
Length (bp)610
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010669 (SMED30010669) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010669 (SMED30010669) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020




SMED30010669

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 2

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
parenchymal cellSMED30010669SMESG000015083.1 dd_Smed_v4_3223_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
whole organism asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010669SMESG000015083.1 dd_Smed_v6_3223_0dd_Smed_v6PMID:29674432
Plass et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010669 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001173:65193..65787 +2930
Homology
BLAST of SMED30010669 vs. Planmine SMEST
Match: SMESG000015083.1 (SMESG000015083.1)

HSP 1 Score: 318.931 bits (816), Expect = 1.444e-110
Identity = 201/202 (99.50%), Postives = 202/202 (100.00%), Query Frame = 3
Query:    3 LDLSENQVSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPNHNGEVWKIVVAILLPFFGLAIIFVCYW 608
            LDLSENQ+SSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRPNHNNSTTNNPNHSTSTYPNYSNTTKTSNSTKNHSTKPPNHNGEVWKIVVAILLPFFGLAIIFVCYW
Sbjct:   94 LDLSENQLSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRPNHNNSTTNNPNHSTSTYPNYSNTTKTSNSTKNHSTKPPNHNGEVWKIVVAILLPFFGLAIIFVCYW 295          
BLAST of SMED30010669 vs. Planmine SMEST
Match: SMESG000015083.1 (SMESG000015083.1)

HSP 1 Score: 318.931 bits (816), Expect = 2.079e-110
Identity = 201/202 (99.50%), Postives = 202/202 (100.00%), Query Frame = 3
Query:    3 LDLSENQVSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPNHNGEVWKIVVAILLPFFGLAIIFVCYW 608
            LDLSENQ+SSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRPNHNNSTTNNPNHSTSTYPNYSNTTKTSNSTKNHSTKPPNHNGEVWKIVVAILLPFFGLAIIFVCYW
Sbjct:  102 LDLSENQLSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFKPSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRPNHNNSTTNNPNHSTSTYPNYSNTTKTSNSTKNHSTKPPNHNGEVWKIVVAILLPFFGLAIIFVCYW 303          
BLAST of SMED30010669 vs. Planmine SMEST
Match: SMESG000070256.1 (SMESG000070256.1)

HSP 1 Score: 78.9518 bits (193), Expect = 4.420e-17
Identity = 51/97 (52.58%), Postives = 65/97 (67.01%), Query Frame = 3
Query:    3 LDLSENQVSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNK----NY-ELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINIS 278
            LDLSENQ+SSLALSG QLSVVNVS NFLSGL+VNLPN+ +L   NN+    N+  L    H      + + S++ ++S P  L+T   NK LTIN+S
Sbjct:   97 LDLSENQLSSLALSGDQLSVVNVSSNFLSGLEVNLPNVLQLIASNNRMGKLNFGNLILDLHKSPSLTVLDVSRNRINSLPVDLMTFNNNKNLTINLS 193          
BLAST of SMED30010669 vs. Planmine SMEST
Match: SMESG000070256.1 (SMESG000070256.1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.877e-17
Identity = 51/97 (52.58%), Postives = 65/97 (67.01%), Query Frame = 3
Query:    3 LDLSENQVSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNK----NY-ELFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINIS 278
            LDLSENQ+SSLALSG QLSVVNVS NFLSGL+VNLPN+ +L   NN+    N+  L    H      + + S++ ++S P  L+T   NK LTIN+S
Sbjct:   94 LDLSENQLSSLALSGDQLSVVNVSSNFLSGLEVNLPNVLQLIASNNRMGKLNFGNLILDLHKSPSLTVLDVSRNRINSLPVDLMTFNNNKNLTINLS 190          
The following BLAST results are available for this feature:
BLAST of SMED30010669 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010669 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 4
Match NameE-valueIdentityDescription
SMESG000015083.11.444e-11099.50SMESG000015083.1[more]
SMESG000015083.12.079e-11099.50SMESG000015083.1[more]
SMESG000070256.14.420e-1752.58SMESG000070256.1[more]
SMESG000070256.14.877e-1752.58SMESG000070256.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010669 ID=SMED30010669|Name=SMED30010669|organism=Schmidtea mediterranea sexual|type=transcript|length=610bp
TTTTGGATCTTAGTGAGAATCAGGTGAGCAGTCTAGCATTATCTGGCGGT
CAACTGTCGGTTGTAAACGTTTCCCGCAACTTTCTCTCAGGATTGGACGT
CAACTTACCGAATCTCAGGAAGCTGTTTGTTGACAATAATAAAAATTATG
AGTTATTTTCATCTCAACATGATTATCTCTTGAATGAACTTCAGAATGAG
AGCAAAGATTGGGTTGATAGCGCACCAACTGCGCTTGTGACAGTTACCCG
CAATAAACCTTTAACGATTAACATCTCTTCTTTGATTAGGCCTGTTTTCA
AGCCTTCACGATTAATTAAACGCAAACAATTTCATCTCTCTTTGACTAAA
TCTACAATGAAAGGAATCTTCACTTTAATTGAATCAAGTGACCGAGTTGC
ATTCCGCCCAAACCACAACAACTCAACTACCAATAACCCTAATCACTCAA
CTTCCACCTACCCCAACTACTCAAACACCACCAAGACCAGCAACTCAACC
AAAAACCATTCTACTAAACCCCCAAATCACAATGGAGAAGTATGGAAAAT
TGTAGTAGCAATCTTACTGCCATTCTTTGGATTAGCAATCATTTTCGTGT
GCTACTGGAC
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protein sequence of SMED30010669-orf-1

>SMED30010669-orf-1 ID=SMED30010669-orf-1|Name=SMED30010669-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=202bp
LDLSENQVSSLALSGGQLSVVNVSRNFLSGLDVNLPNLRKLFVDNNKNYE
LFSSQHDYLLNELQNESKDWVDSAPTALVTVTRNKPLTINISSLIRPVFK
PSRLIKRKQFHLSLTKSTMKGIFTLIESSDRVAFRPNHNNSTTNNPNHST
STYPNYSNTTKTSNSTKNHSTKPPNHNGEVWKIVVAILLPFFGLAIIFVC
YW
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR032675LRR_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3DG3DSA:3.80.10.10coord: 1..95
e-value: 6.8E-6
score: 26.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..175
NoneNo IPR availableSUPERFAMILYSSF52075Outer arm dynein light chain 1coord: 1..53