SMED30010667

Overview
NameSMED30010667
Smed IDSMED30010667
Length (bp)498
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010667 (SMED30010667) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010667 (SMED30010667) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010667 (SMED30010667) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010667 (SMED30010667) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010667

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
gutSMED30010667h1SMcG0020483 dd_Smed_v4_2672_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010667h1SMcG0020483 dd_Smed_v4_2672_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010667h1SMcG0020483 dd_Smed_v4_2672_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010667h1SMcG0020483 dd_Smed_v4_2672_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010667h1SMcG0020483 Contig45059uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010667h1SMcG0020483 Contig45059newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30010667h1SMcG0020483 dd_Smed_v6_2672_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010667 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000281:28979..31007 +2360
Homology
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BLAST of SMED30010667 vs. TrEMBL
Match: A0A2B4RN34 (Putative serine/threonine-protein kinase OS=Stylophora pistillata OX=50429 GN=AWC38_SpisGene17416 PE=4 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.692e-7
Identity = 36/126 (28.57%), Postives = 68/126 (53.97%), Query Frame = 3 
Query:    3 GKVLFINLLVNNRVLPVSIRSSSVCQVRLTYGAVERA--EVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKKT 374
            GK   INLL+N RVLP S+R +++    ++YG  + A     D++     +   EF  ++K+Y++K  +  +  C    I L + +L+GG+ I D+ G+G++E     V+K L      ++++  +
Sbjct:   83 GKSSLINLLLNERVLPSSVRQNTLTICEISYGPEKEAVLHFHDQSKPASILKGEEFEKQMKTYIQKSTEDKDW-CEKIEIRLPNTLLKGGIVIVDSPGIGDTENIASIVMKYLPQANAFIYVINSS 207
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BLAST of SMED30010667 vs. Planmine SMEST
Match: SMESG000010252.1 (SMESG000010252.1)

HSP 1 Score: 240.35 bits (612), Expect = 3.206e-87
Identity = 120/123 (97.56%), Postives = 120/123 (97.56%), Query Frame = 3 
Query:    3 GKVLFINLLVNNRVLPVSIRSSSVCQVRLTYGAVERAEVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKK 371
            GK  FINLLVNNRVLPVSIRSSSVCQVRLTYGAVE AEVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKK
Sbjct:  159 GKSSFINLLVNNRVLPVSIRSSSVCQVRLTYGAVECAEVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKK 281

HSP 2 Score: 99.3673 bits (246), Expect = 3.206e-87
Identity = 44/49 (89.80%), Postives = 46/49 (93.88%), Query Frame = 2 
Query:  347 GCSFLVKENNPIGDNTVGGLSKVICHIATNGIPGGSMHFIINMCDLLVK 493
            G  FL+K+NNPIGDNTVGGLSKVICHIATNGIPGGSMHFIINMCDLL K
Sbjct:  274 GVLFLLKKNNPIGDNTVGGLSKVICHIATNGIPGGSMHFIINMCDLLEK 322
[Close]
BLAST of SMED30010667 vs. Planmine SMEST
Match: SMESG000010251.1 (SMESG000010251.1)

HSP 1 Score: 155.221 bits (391), Expect = 1.090e-54
Identity = 74/123 (60.16%), Postives = 98/123 (79.67%), Query Frame = 3 
Query:    3 GKVLFINLLVNNRVLPVSIRSSSVCQVRLTYGAVERAEVVDRNNTTIKIGKNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGESEMFNDQVLKDLNDITGVLFLLKK 371
            GK  FINLLV   +LPVSI++++ CQVRLT+G  E AEVV+R+ T  +I K +F  +L +Y+EK D+R ++SCNS TI + S IL+GGLDIYDTIGLGES+ FN+ +LKDL++ITGV+FLLKK
Sbjct:  160 GKSSFINLLVKRDILPVSIKTNTACQVRLTFGENEYAEVVERDGTKKRIEKEKFSKELTAYIEKYDERDQVSCNSVTINMESSILQGGLDIYDTIGLGESDFFNEHLLKDLSNITGVIFLLKK 282

HSP 2 Score: 75.8702 bits (185), Expect = 1.090e-54
Identity = 33/49 (67.35%), Postives = 40/49 (81.63%), Query Frame = 2 
Query:  347 GCSFLVKENNPIGDNTVGGLSKVICHIATNGIPGGSMHFIINMCDLLVK 493
            G  FL+K+NNPIG+NTVGGLSK+I HI TN +P  SM+F+INMCD L K
Sbjct:  275 GVIFLLKKNNPIGENTVGGLSKIIDHIMTNDVPSSSMYFVINMCDQLDK 323
The following BLAST results are available for this feature:
BLAST of SMED30010667 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 1
Match NameE-valueIdentityDescription
A0A2B4RN341.692e-728.57Putative serine/threonine-protein kinase OS=Stylop... [more]
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BLAST of SMED30010667 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010667 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000010252.13.206e-8797.56SMESG000010252.1[more]
SMESG000010251.11.090e-5460.16SMESG000010251.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010667 ID=SMED30010667|Name=SMED30010667|organism=Schmidtea mediterranea sexual|type=transcript|length=498bp
AGGGAAAAGTTCTTTTCATAAACCTCCTGGTTAATAATCGAGTCCTTCCG
GTTTCAATAAGAAGTAGCTCAGTTTGTCAAGTAAGACTGACATACGGTGC
CGTTGAGCGTGCTGAGGTAGTTGATCGGAATAACACTACGATCAAGATTG
GAAAGAATGAGTTTGGAACCAAACTCAAATCATATCTAGAAAAGCTAGAT
GATCGAACCGAAATTTCATGCAATTCTGCGACCATTTATTTGAATTCTCC
AATTCTAGAGGGTGGATTGGATATCTACGACACGATTGGACTGGGAGAGT
CAGAAATGTTTAATGATCAAGTACTGAAAGATTTAAATGATATAACGGGT
GTTCTTTTCTTGTTAAAGAAAACAACCCGATCGGAGACAATACTGTTGGA
GGGCTTTCGAAGGTAATTTGTCATATCGCAACGAATGGGATTCCCGGCGG
ATCTATGCACTTTATTATCAATATGTGCGATTTATTGGTAAAAATTTT
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protein sequence of SMED30010667-orf-1

>SMED30010667-orf-1 ID=SMED30010667-orf-1|Name=SMED30010667-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=138bp
GKVLFINLLVNNRVLPVSIRSSSVCQVRLTYGAVERAEVVDRNNTTIKIG
KNEFGTKLKSYLEKLDDRTEISCNSATIYLNSPILEGGLDIYDTIGLGES
EMFNDQVLKDLNDITGVLFLLKKTTRSETILLEGFRR*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000026gut
PLANA:0000101muscle cell
PLANA:0003001lateral region of the whole animal
PLANA:0003116parenchymal cell
Vocabulary: cellular component
TermDefinition
GO:0016020membrane
GO:0016021integral component of membrane