SMED30010654

Overview
NameSMED30010654
Smed IDSMED30010654
Length (bp)2801
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010654 (SMED30010654) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010654 (SMED30010654) in cells (dots) of each of the 12 neoblast clusters.

 

back to top

 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010654 (SMED30010654) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010654 (SMED30010654) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top

 

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020



SMED30010654

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 13

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30010654SMESG000022828.1 Contig1050uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30010654SMESG000022828.1 Contig1050newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30010654SMESG000054525.1 Contig1050uc_Smed_v2PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30010654SMESG000054525.1 Contig1050newmark_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
nervous systemSMED30010654SMESG000022832.1 SMESG000022828.1 dd_Smed_v4_3053_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
gutSMED30010654SMESG000022832.1 SMESG000022828.1 dd_Smed_v4_3053_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010654SMESG000022832.1 SMESG000022828.1 dd_Smed_v4_3053_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010654SMESG000022832.1 SMESG000022828.1 dd_Smed_v4_3053_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30010654SMESG000022828.1 Contig33968uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30010654SMESG000022828.1 Contig33968uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30010654SMESG000022828.1 Contig33968newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30010654SMESG000022828.1 Contig33968newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30010654SMESG000022832.1 SMESG000022828.1 dd_Smed_v4_3053_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010654 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.003047:2826..8194 +11700
Homology
[Close]
BLAST of SMED30010654 vs. Ensembl Zebrafish
Match: FO834800.1 (E3 ubiquitin-protein ligase RNF31 [Source:NCBI gene;Acc:100334178])

HSP 1 Score: 50.8322 bits (120), Expect = 8.485e-6
Identity = 33/132 (25.00%), Postives = 49/132 (37.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C+ C+  N      C TC   DR R         +     + +  WQC  C+ +N  S++ CE C       R                  P    P    +S    D+ W C  C+YVN   ++VC++C
Sbjct:  298 WQCSYCTTVNSMLQVLCETC---DRPRLSSAAPSLQEESSQPSTISEWQCKSCTVVNAGSSILCEVCERPRLATR------------------PPAVPPRATPSSSALMDSQWICQFCTYVNRTPSTVCDMC 408
[Close]
BLAST of SMED30010654 vs. Ensembl Xenopus
Match: rnf31 (ring finger protein 31 [Source:NCBI gene;Acc:100036616])

HSP 1 Score: 56.225 bits (134), Expect = 2.426e-7
Identity = 44/162 (27.16%), Postives = 65/162 (40.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETC-----------------GDTARNKRKANELQANKISEMKTLF----------EPGENAPVPNNASR---IDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C  C F+NK  +  CV C   +R RG  +   +  L       DSW+C  C+  N  SAV C  C                     R+ ++ N+     +SE   LF          EP    P+ ++  +   I     WQC+ C++ N ++  VCEIC
Sbjct:  211 WRCYSCHFENKGGSVLCVVC---ERPRGCTSSLSNLSLGSTLQ-RDSWECQACTLQNSASAVLCAVCDRPRLASKPAQDGYDSVALLLRDIQRDNKSITCPVSEKNGLFAMKNHQESMHEPESIGPLIDHTRQEEEIAKSEGWQCSHCTFFNTRNGRVCEIC 368
[Close]
BLAST of SMED30010654 vs. Ensembl Xenopus
Match: rnf31 (ring finger protein 31 [Source:NCBI gene;Acc:100036616])

HSP 1 Score: 56.225 bits (134), Expect = 2.534e-7
Identity = 44/162 (27.16%), Postives = 65/162 (40.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETC-----------------GDTARNKRKANELQANKISEMKTLF----------EPGENAPVPNNASR---IDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C  C F+NK  +  CV C   +R RG  +   +  L       DSW+C  C+  N  SAV C  C                     R+ ++ N+     +SE   LF          EP    P+ ++  +   I     WQC+ C++ N ++  VCEIC
Sbjct:  212 WRCYSCHFENKGGSVLCVVC---ERPRGCTSSLSNLSLGSTLQ-RDSWECQACTLQNSASAVLCAVCDRPRLASKPAQDGYDSVALLLRDIQRDNKSITCPVSEKNGLFAMKNHQESMHEPESIGPLIDHTRQEEEIAKSEGWQCSHCTFFNTRNGRVCEIC 369
[Close]
BLAST of SMED30010654 vs. Ensembl Xenopus
Match: rnf31 (ring finger protein 31 [Source:NCBI gene;Acc:100036616])

HSP 1 Score: 56.225 bits (134), Expect = 2.655e-7
Identity = 44/162 (27.16%), Postives = 65/162 (40.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETC-----------------GDTARNKRKANELQANKISEMKTLF----------EPGENAPVPNNASR---IDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C  C F+NK  +  CV C   +R RG  +   +  L       DSW+C  C+  N  SAV C  C                     R+ ++ N+     +SE   LF          EP    P+ ++  +   I     WQC+ C++ N ++  VCEIC
Sbjct:  231 WRCYSCHFENKGGSVLCVVC---ERPRGCTSSLSNLSLGSTLQ-RDSWECQACTLQNSASAVLCAVCDRPRLASKPAQDGYDSVALLLRDIQRDNKSITCPVSEKNGLFAMKNHQESMHEPESIGPLIDHTRQEEEIAKSEGWQCSHCTFFNTRNGRVCEIC 388
[Close]
BLAST of SMED30010654 vs. Ensembl Xenopus
Match: rnf31 (ring finger protein 31 [Source:NCBI gene;Acc:100036616])

HSP 1 Score: 56.225 bits (134), Expect = 2.660e-7
Identity = 44/162 (27.16%), Postives = 65/162 (40.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETC-----------------GDTARNKRKANELQANKISEMKTLF----------EPGENAPVPNNASR---IDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C  C F+NK  +  CV C   +R RG  +   +  L       DSW+C  C+  N  SAV C  C                     R+ ++ N+     +SE   LF          EP    P+ ++  +   I     WQC+ C++ N ++  VCEIC
Sbjct:  211 WRCYSCHFENKGGSVLCVVC---ERPRGCTSSLSNLSLGSTLQ-RDSWECQACTLQNSASAVLCAVCDRPRLASKPAQDGYDSVALLLRDIQRDNKSITCPVSEKNGLFAMKNHQESMHEPESIGPLIDHTRQEEEIAKSEGWQCSHCTFFNTRNGRVCEIC 368
[Close]
BLAST of SMED30010654 vs. Ensembl Xenopus
Match: rnf31 (ring finger protein 31 [Source:NCBI gene;Acc:100036616])

HSP 1 Score: 56.225 bits (134), Expect = 2.692e-7
Identity = 44/162 (27.16%), Postives = 65/162 (40.12%), Query Frame = 3 
Query: 1332 WSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETC-----------------GDTARNKRKANELQANKISEMKTLF----------EPGENAPVPNNASR---IDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C  C F+NK  +  CV C   +R RG  +   +  L       DSW+C  C+  N  SAV C  C                     R+ ++ N+     +SE   LF          EP    P+ ++  +   I     WQC+ C++ N ++  VCEIC
Sbjct:  205 WRCYSCHFENKGGSVLCVVC---ERPRGCTSSLSNLSLGSTLQ-RDSWECQACTLQNSASAVLCAVCDRPRLASKPAQDGYDSVALLLRDIQRDNKSITCPVSEKNGLFAMKNHQESMHEPESIGPLIDHTRQEEEIAKSEGWQCSHCTFFNTRNGRVCEIC 362
[Close]
BLAST of SMED30010654 vs. Ensembl Mouse
Match: Ranbp2 (RAN binding protein 2 [Source:MGI Symbol;Acc:MGI:894323])

HSP 1 Score: 52.373 bits (124), Expect = 4.088e-6
Identity = 80/344 (23.26%), Postives = 120/344 (34.88%), Query Frame = 3 
Query: 1044 SNKTISAKTVI----ASNTNLSTLANTKSIVCDNK---WACSKCSYENETSDVKCVTCSHTDSNGGKETES---RSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGD-TARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKE-------AEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGA 2021
            SNK +    ++    A  T L    +  + +  NK   W CS C   NE +  +C+ C +T S       S       LP+S          S   +    ++G  W C+ C  +N++S   CV C     N GK+ K               W C+ CS  N+  A+ C  C +    +   A        SEM   F  G              +  W C+ C   NE S + C  C    + P K+       +     ET R  K G    F   + +           W C  CS  N+  S +C  C+   P   K  +    F     +Q+N+  G+
Sbjct: 1376 SNKELLGPPLVENGFAPKTGLENAQDRFATMTANKEGHWDCSVCLVRNEPTVSRCIACQNTKSASSFVQTSFKFGQGDLPKSVD--------SDFRSVFSKKEGQ-WECSVCLVRNERSAKKCVAC----ENPGKQFKE--------------WHCSLCSVKNEAHAIKCVACNNPVTPSLSTAPPSFKFGTSEMSKPFRIG------FEGMFAKKEGQWDCSLCFVRNEASATHCIAC----QYPNKQNQPTSCVSAPASSETSRSPKSGFEGLFPKKEGE-----------WECAVCSVQNESSSLKCVACEASKPTH-KPHEAPSAFTVGSKSQSNESAGS 1670
[Close]
BLAST of SMED30010654 vs. TrEMBL
Match: A0A1X7UH10 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 3.739e-10
Identity = 61/240 (25.42%), Postives = 102/240 (42.50%), Query Frame = 3 
Query: 1137 WACSKCSYENETSDVKCVTC----SHTDSNGGKETESRSNKLPESQ---SITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGY---IDRNRGKENKSRSD----------------------KLLEMQN--FVDSWQCAKCSYINKTSAVTCETCGDTARNKRKA---------NELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C+ C+YEN+     C  C    S  DS+  K  + R+ K  E +    +TV    +S   +  R++  ++WSC  C++KN ++   C  CG+   ID  + K+++  +D                      KL E +    V  W C KC+++N    + CE CG T R    +          E+     + ++ L    EN+P+  N   ++  + W C  C+Y N K    C +C
Sbjct:    7 WTCTSCTYENQLKSQYCSMCNEERSFKDSDPMKRRQKRTKKEDEEEIGPEVTVMDRLSSFSRSLFRLK-SSHWSCPGCTYKNNEADTQCAVCGHDKPIDVKQSKDDRQSNDTNTGSITSSISNGISSGFKKLKKLFEPEPEVTVTGWTCRKCTFVNHPDLLECEQCGSTGRRHPLSLDHILPTDDGEVLFGDPAVLRELINKLENSPL--NEYSLEETDKWVCPACTYHN-KGGERCGVC 242
[Close]
BLAST of SMED30010654 vs. TrEMBL
Match: A0A1A8H6N3 (RANBP2-like and GRIP domain containing 2 (Fragment) OS=Nothobranchius korthausae OX=1143690 GN=RGPD2 PE=4 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 9.036e-10
Identity = 73/340 (21.47%), Postives = 109/340 (32.06%), Query Frame = 3 
Query: 1128 DNKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSN----------------------------------KLPESQSI-TVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCG----------YIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDND--------NTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAE-----GIPEETWRCNKCGNNNSFDVYKCQKCREIAE-------FADK---WVCGQCSFYNDKLSPRCGVCDMDNP 1943
            +  W CS C   N+ +DV+C+ C H +     + E + +                                   +P S +  T + ++A+T  +      G+ W C +CS KN+ S   C++C            +      +   +     +       W C KCS  N+ SA  C +C   A      + L     S M       E      + +  D+           W C  C   NE S   C  C +   NP   +            W CN C   N     +C  CR   E       FA K   W C  C   ND  + +C  C   NP
Sbjct: 1288 EGDWECSVCCVRNKPTDVRCLACQHANPQSSSKPEIQPSGETKGTQFTFKFGTDASNPSSSGSTFSGFGAFGASIPSSFTFGTNSSKSANTKPSAFTFGFGSQWDCKKCSTKNEASVESCISCKAPKIVAPTSVMVQTTPAVDADVKDSGFAQFSKESGQWDCNKCSSKNEVSAECCVSC--KAPKTAALHMLSHAITSAMAQTTPAAEAPAAQPSVAVSDSGFAQFSKKPGQWDCALCEVRNEASADKCAACQS--PNPAATSALGSDFAKKNGQWHCNACLVRNEASATECVSCRTPHENPSLATIFARKEGEWDCDTCLVRNDASTSKCQACQAPNP 1623
[Close]
BLAST of SMED30010654 vs. TrEMBL
Match: A0A1A8ETE8 (RANBP2-like and GRIP domain containing 2 (Fragment) OS=Nothobranchius korthausae OX=1143690 GN=RGPD2 PE=4 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.294e-9
Identity = 73/340 (21.47%), Postives = 109/340 (32.06%), Query Frame = 3 
Query: 1128 DNKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSN----------------------------------KLPESQSI-TVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCG----------YIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDND--------NTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAE-----GIPEETWRCNKCGNNNSFDVYKCQKCREIAE-------FADK---WVCGQCSFYNDKLSPRCGVCDMDNP 1943
            +  W CS C   N+ +DV+C+ C H +     + E + +                                   +P S +  T + ++A+T  +      G+ W C +CS KN+ S   C++C            +      +   +     +       W C KCS  N+ SA  C +C   A      + L     S M       E      + +  D+           W C  C   NE S   C  C +   NP   +            W CN C   N     +C  CR   E       FA K   W C  C   ND  + +C  C   NP
Sbjct: 1173 EGDWECSVCCVRNKPTDVRCLACQHANPQSSSKPEIQPSGETKGTQFTFKFGTDASNPSSSGSTFSGFGAFGASIPSSFTFGTNSSKSANTKPSAFTFGFGSQWDCKKCSTKNEASVESCISCKAPKIVAPTSVMVQTTPAVDADVKDSGFAQFSKESGQWDCNKCSSKNEVSAECCVSC--KAPKTAALHMLSHAITSAMAQTTPAAEAPAAQPSVAVSDSGFAQFSKKPGQWDCALCEVRNEASADKCAACQS--PNPAATSALGSDFAKKNGQWHCNACLVRNEASATECVSCRTPHENPSLATIFARKEGEWDCDTCLVRNDASTSKCQACQAPNP 1508
[Close]
BLAST of SMED30010654 vs. TrEMBL
Match: A0A1X7SKP5 (Uncharacterized protein OS=Amphimedon queenslandica OX=400682 PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 2.604e-9
Identity = 60/240 (25.00%), Postives = 102/240 (42.50%), Query Frame = 3 
Query: 1137 WACSKCSYENETSDVKCVTC----SHTDSNGGKETESRSNKLPESQ---SITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGY---IDRNRGKENKSRSD----------------KLLEMQNF--------VDSWQCAKCSYINKTSAVTCETCGDTARNKRKA---------NELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEIC 1727
            W C+ C+YEN+     C  C    S  DS+  K  + R+ K  E +    +TV    +S   +  R++  ++WSC +C++KN ++   C  CG    ID  + K++K  +D                   +++ F        V  W C KC+++N    + CE CG T R    +          E+     + ++ L    EN+P+  N   ++  + W C  C+Y N K    C +C
Sbjct:    7 WTCASCTYENQLKSQYCSMCNKERSIKDSDPMKRRQKRTKKEDEEEIGPEVTVMDRLSSFSRSLFRLK-SSHWSCPECTYKNNEADTQCGMCGQDKPIDIKQFKDDKQSNDTNTGSITSSISSGISSGFKKLKKFFEPDPEVAVTGWTCRKCTFVNHPDLLECEQCGSTQRRHPLSLDHILPTDDGEVLFGDPAVLRELINKLENSPL--NEYSLEETDRWVCPACTYHN-KGGERCGVC 242
[Close]
BLAST of SMED30010654 vs. TrEMBL
Match: A0A0N0P2D5 (Uncharacterized protein OS=Leptomonas seymouri OX=5684 GN=ABL78_8162 PE=4 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.268e-8
Identity = 66/275 (24.00%), Postives = 103/275 (37.45%), Query Frame = 3 
Query: 1137 WACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDK------LLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNP 1943
            W C+ C++ N+   VKC  C  T    G    + S+  P   +      +AS L+  +R      W C  C   N  S   C  C Y  R+ G   ++ S        ++    F   W+C+ C  +N  S+  C+ C    RN    +E  A   S        G + P+P           W C+ C++ N  S +VC  C +     G+    +  +  +  K  +N      K +   E A    +W C  C+F N     RC +C    P
Sbjct:  206 WGCAVCTFRNKLGVVKCEMCGSTRP--GYTAPATSSPSPPVAATPSAQPSASALSPPSRA-GVTAWQCGMCLAPNDASAERCKVC-YAYRSSGTPVQTTSSAGGGGMGVVSNTPFTTRWKCSVCGEVNAPSSANCKICHGFQRNGTPVSEESAPNSSSNHG----GGDPPLP---------TAWACSVCTFENPTSAAVCTACES-----GQRPRHLAPKRDKEKKHASNKHHGGEKKKAEEEGA--VRQWPCPTCTFLNPVGRKRCDMCGAKRP 456
[Close]
BLAST of SMED30010654 vs. Ensembl Medaka
Match: si:ch1073-55a19.2 (RANBP2-like and GRIP domain-containing protein 8 [Source:NCBI gene;Acc:101160447])

HSP 1 Score: 58.5362 bits (140), Expect = 3.664e-8
Identity = 94/452 (20.80%), Postives = 151/452 (33.41%), Query Frame = 3 
Query:  741 YSTKKNISFEKPDKKKEKSQNLQTYN-FESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRA-DGFGNKSNKTISAKTVIASNTNLSTLANTKSIV---CDNK----WACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKEN--------------KSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEE------------------TWRCNKCGNNNSFDVYKCQKCRE----------IAEFADKWVCGQCSFYNDKLSPRCGVCDMDNP 1943
            +   K ++   P+K+ EK  + +  +   ++  ++E DWE  D     N   + R  KA +  N     E++  + S  T  + + +           GFG   +   S+ T    ++ L   A TK+I     D+K    W C KCS +NETS   C++C  + +   K  +S      +  +       A  L+     + G  W C  C  +N+ +   CV C    +   + +                  D   +       W C  C   N ++A  C  C   A  K+      A  +    T+ +P   AP    A        W C  C   NE +   C  C   + +  KE    P                     W C  C   N     KC  C              +   +W C  C   N+  + +C  C   NP
Sbjct: 1297 FEEAKKVALASPEKQPEKKSSSKDSDSLAARFALKEGDWE-CDVCCVVNKPSDLR-CKACQTANPSSKAEVQAPKASAFTFKFGTDSSKQSSSGSTFAGFGGFGSSVPSSFTFGMGSSKLPDAA-TKAISFGSLDDKRSAQWNCEKCSTKNETSADVCLSCKTSKTATKKTVQSSPTAAVQPSAF------APDLSAQFGKKPGQ-WDCNVCEVRNESAAERCVACKTAAKKTVQSSPAAAVQPAAAVQPAAFAPDLRAQFGKKPGQWDCNVCEVRNDSAAERCVACKTAA--KKTVQSSPAAAVQPAATV-QPAAFAP-DLRAQFGKKPGQWDCNVCEVRNESAAERCVAC---NTSKAKETATAPVAPSLPPAPEITADSSKVGGQWDCKSCLVRNEASATKCVCCSAPNDTLSLEAIFGKNQGEWDCDACLVRNEASASKCVACQAANP 1731
[Close]
BLAST of SMED30010654 vs. Ensembl Medaka
Match: si:ch1073-55a19.2 (RANBP2-like and GRIP domain-containing protein 8 [Source:NCBI gene;Acc:101160447])

HSP 1 Score: 58.5362 bits (140), Expect = 3.693e-8
Identity = 94/452 (20.80%), Postives = 151/452 (33.41%), Query Frame = 3 
Query:  741 YSTKKNISFEKPDKKKEKSQNLQTYN-FESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRA-DGFGNKSNKTISAKTVIASNTNLSTLANTKSIV---CDNK----WACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKEN--------------KSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEE------------------TWRCNKCGNNNSFDVYKCQKCRE----------IAEFADKWVCGQCSFYNDKLSPRCGVCDMDNP 1943
            +   K ++   P+K+ EK  + +  +   ++  ++E DWE  D     N   + R  KA +  N     E++  + S  T  + + +           GFG   +   S+ T    ++ L   A TK+I     D+K    W C KCS +NETS   C++C  + +   K  +S      +  +       A  L+     + G  W C  C  +N+ +   CV C    +   + +                  D   +       W C  C   N ++A  C  C   A  K+      A  +    T+ +P   AP    A        W C  C   NE +   C  C   + +  KE    P                     W C  C   N     KC  C              +   +W C  C   N+  + +C  C   NP
Sbjct: 1297 FEEAKKVALASPEKQPEKKSSSKDSDSLAARFALKEGDWE-CDVCCVVNKPSDLR-CKACQTANPSSKAEVQAPKASAFTFKFGTDSSKQSSSGSTFAGFGGFGSSVPSSFTFGMGSSKLPDAA-TKAISFGSLDDKRSAQWNCEKCSTKNETSADVCLSCKTSKTATKKTVQSSPTAAVQPSAF------APDLSAQFGKKPGQ-WDCNVCEVRNESAAERCVACKTAAKKTVQSSPAAAVQPAAAVQPAAFAPDLRAQFGKKPGQWDCNVCEVRNDSAAERCVACKTAA--KKTVQSSPAAAVQPAATV-QPAAFAP-DLRAQFGKKPGQWDCNVCEVRNESAAERCVAC---NTSKAKETATAPVAPSLPPAPEITADSSKVGGQWDCKSCLVRNEASATKCVCCSAPNDTLSLEAIFGKNQGEWDCDACLVRNEASASKCVACQAANP 1731
[Close]
BLAST of SMED30010654 vs. Ensembl Medaka
Match: zranb1a (zinc finger RANBP2-type containing 1 [Source:NCBI gene;Acc:101175114])

HSP 1 Score: 50.447 bits (119), Expect = 8.997e-6
Identity = 52/205 (25.37%), Postives = 81/205 (39.51%), Query Frame = 3 
Query: 1131 NKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVP-----NNASRID-NDNTWQCTQCSYVNEKSTSVCEIC 1727
             KWAC  C+YEN  S  KC  CS     G   TE      P+         + S   +  R + G+  S   C   +              R R K +         M    + W C  C+Y+N   AV C  C    +  R ++  ++ + S  +    PG ++PV      N+ +R++ +   W CT C+Y N  +T+ C +C
Sbjct:   11 GKWACEYCTYENWPSANKCTMCSAPRMRGPIITEEPYKTGPDL--------DPSPEWDPPRTDRGS--SLLICPDSSA-------------RPRVKPSS--------MAEIANKWSCHMCTYLNWPRAVRCTQC--LCQRPRTSSPTESPQTSGCR----PGVHSPVDTCEEYNDRNRLNTHQQHWTCTVCTYQNWPTTTACVVC 178
[Close]
BLAST of SMED30010654 vs. Planmine SMEST
Match: SMESG000022828.1 (SMESG000022828.1)

HSP 1 Score: 1433.7 bits (3710), Expect = 0.000e+0
Identity = 734/839 (87.49%), Postives = 746/839 (88.92%), Query Frame = 3 
Query:  231 MNGQNFESLVECIETDENTEPLKEKIRQKISCVNQIDYFPVEVQFKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMPFDKYMEKIPKMNDSYIDESQLVEIIAIYVIIYSLLLNQEKILDKDDNMVCIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRAD---------------------------------------------------GFGNKSNKTISAKTVIASNTNLSTLANTKSIVCDNKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACKVSQKTVYNSLPSNNAIKSSPKPGSGDXXXXXXXXXXXXXLSLQNANVTQLAPSSGSGDTKRKSCKNCRKEFQPNSNNNWCITCEQMKWFCLNESCDSVNFKVEFCKKCQVKKGSDLSNYKAFPISLQGKEVLSYQCGYCYQIIRSTINSCNSCKNPRYLSNLVQCKCGWNYKKIENDCGKCKEKFSEKL 2594
            MNGQNFESLVECIETDENTEPLKEKIRQKISC+NQIDYFPVEVQFKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMPFDKYMEKIPKMNDSYIDESQLVEIIAIYVIIYSLLLNQEKILDKDDNMVCIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRAD                                                   GFGNKSNKTISAKTVIASNTNLSTLANTKSIVCDNKWACSKCSYENETSDVKCVTCSHTD NGGKETES SNKLPESQSITVTHENA TLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGY DRNRGKE KSRSDKLLEMQN VDSW+CAKCSYINK SAVTCETCGDTARNKRKANELQANKISEMKTLFEP ENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCR IAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGAC++SQKT Y SLP   AIK SP PGSG AKKNIN+ K GNNLSLQNANVTQ A S GSGDTKRKSCK+CRKE QPNSNNNWCITCE+MKWFCLNE+CDSVNFKVEFCKKCQVKK               GKEVLSYQCGYCYQIIRS +NSCNSCKNP+Y+SNLVQCKCGWNYKK EN+CGKC EKF EKL
Sbjct:    1 MNGQNFESLVECIETDENTEPLKEKIRQKISCINQIDYFPVEVQFKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMPFDKYMEKIPKMNDSYIDESQLVEIIAIYVIIYSLLLNQEKILDKDDNMVCIYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESKQTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRADRFGKESKTNNIKYKDEKKKHKINNDNSDQPSVKTPDCYSLYERTCEDQRADGFGNKSNKTISAKTVIASNTNLSTLANTKSIVCDNKWACSKCSYENETSDVKCVTCSHTDRNGGKETESGSNKLPESQSITVTHENAPTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYTDRNRGKETKSRSDKLLEMQNSVDSWECAKCSYINKISAVTCETCGDTARNKRKANELQANKISEMKTLFEPAENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCRGIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACEISQKTDYYSLPIRIAIKPSPSPGSGGAKKNINDRKTGNNLSLQNANVTQPALSPGSGDTKRKSCKHCRKELQPNSNNNWCITCEKMKWFCLNEACDSVNFKVEFCKKCQVKK---------------GKEVLSYQCGYCYQIIRSILNSCNSCKNPKYISNLVQCKCGWNYKKTENNCGKCNEKFCEKL 824
[Close]
BLAST of SMED30010654 vs. Planmine SMEST
Match: SMESG000022832.1 (SMESG000022832.1)

HSP 1 Score: 800.045 bits (2065), Expect = 0.000e+0
Identity = 407/450 (90.44%), Postives = 414/450 (92.00%), Query Frame = 3 
Query: 1245 KLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACKVSQKTVYNSLPSNNAIKSSPKPGSGDXXXXXXXXXXXXXLSLQNANVTQLAPSSGSGDTKRKSCKNCRKEFQPNSNNNWCITCEQMKWFCLNESCDSVNFKVEFCKKCQVKKGSDLSNYKAFPISLQGKEVLSYQCGYCYQIIRSTINSCNSCKNPRYLSNLVQCKCGWNYKKIENDCGKCKEKFSEKL 2594
            KLPESQSITVTHENA TLNNTNRIEDGNYWSCTQCSFKNK STFGCVTCGYIDRN+ KENKSRSDKLL+MQN VD WQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDN NTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKC KCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNP LIKCRKCRKGFVNAPNNQNNDICGACKVSQKT Y SLPSNNAIK SP PGSG AKKNIN+ K GNNLSLQNANVTQ A SSGSGDTKRKSCKNCRKE QPNSNNNWCITCE+MKWFCLNE+CDSVNF  +FCKKCQVKK               GKEVLSYQCGYCYQIIRSTINSCNSCKNPRY+ NLVQCKCGWNYKKIENDCGKCKEKFSEKL
Sbjct:  215 KLPESQSITVTHENAPTLNNTNRIEDGNYWSCTQCSFKNKNSTFGCVTCGYIDRNKVKENKSRSDKLLKMQNSVDFWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEPGENAPVPNNASRIDNYNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCHKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPHLIKCRKCRKGFVNAPNNQNNDICGACKVSQKTDYYSLPSNNAIKPSPSPGSGGAKKNINDRKTGNNLSLQNANVTQPALSSGSGDTKRKSCKNCRKELQPNSNNNWCITCEKMKWFCLNEACDSVNFNKKFCKKCQVKK---------------GKEVLSYQCGYCYQIIRSTINSCNSCKNPRYIVNLVQCKCGWNYKKIENDCGKCKEKFSEKL 649
[Close]
BLAST of SMED30010654 vs. Planmine SMEST
Match: SMESG000022833.1 (SMESG000022833.1)

HSP 1 Score: 125.561 bits (314), Expect = 1.494e-29
Identity = 98/321 (30.53%), Postives = 149/321 (46.42%), Query Frame = 3 
Query: 1473 WQCAKCSY-INKTSAVTCETCGDTARNKRKANELQANKIS--EMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICFNIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACKVSQKTVYNSLPSNNAIKSSPKPGSGDXXXXXXXXXXXXXLSLQNANVTQLAPSSGSGDTKRKSCKNCRKEFQPNSNNNWCITCEQMKWFCLNESCDSVNFKV-EFCKKCQVKKGSDLSNYKAFPISLQG-KEV 2420
            W+C  CS+  NK     C  C    +++  +     +KIS  E K L E  EN  +  N+ +      W C QCS+ N+K+  +C +C+    NP +      E+TW C+KC   N FD Y+C +C  +A F DKWVC +CSF+N+KL   C VCD+ N  LIKC+KC K   N    Q+N+ C +C   ++  Y           +P P +  +    +  +     S     + + +      D+K K                      Q  W+C+N+ C ++NF + ++C KC VK     SN  +F I L+  KEV
Sbjct:  311 WECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEK-ENFNIEGNSDK------WSCNQCSFKNDKTDLLCSLCYA--NNPYENISHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEK---NDVIEQDNEFCTSC--IKENDYRGY-------GTPTPVNDISSNKSSRNEQDGKYSKNRIPIEEASNKITGKDSKLK----------------------QFHWYCINKKCSAINFSMAKYCSKCFVKNECASSN--SFFIVLKNDKEV 586

HSP 2 Score: 73.1738 bits (178), Expect = 4.909e-13
Identity = 120/512 (23.44%), Postives = 208/512 (40.62%), Query Frame = 3 
Query:  294 LKEKIRQKISCVNQIDYFPVEVQFKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMPFDKYMEKIP------KMNDSYID---------ESQLVEIIAIYVIIYSLLLNQEKILDKDDNMV---CIYCDNLLGDGICEVCDDTRDYSTKKN---ISFEKP----DKKKEKSQNLQTYNFESKQTIEEIDWENMDKI-TKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTTDYYSLNETTCEDQRADGFGNKSNKTISAKTV--IASNTNLSTLANTKSIVCDNK------WACSKCSYENETSDVKCVTCSHTDSNGGKETESRS-NKLPESQS-ITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTC----GYIDRNRGKEN--------------KSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLF---------EPGENAPVPNN 1640
            LK+ ++  IS +N +D F              +  D + LY  L  +K I+QL  N Y +NN LY K C  N         +Y+EKI        +  +Y+D         E+ L+E+++ Y+II+ +L  +E      DN++   CIYCD ++   IC VC+        K+   ++F+ P    +    K+Q+L + +      +E +     D I TK N + N     +     I     ++ +  S     ++  N T+      D FG   +K  ++K    + S+ N+S L+    +  + +      W C  CS+      ++  +    D     ++E+++ +K+  ++  I +  EN +   N+++      WSC QCSFKN K+   C  C     Y + +   E+              + R  +   +  F D W C KCS+ N+     C  C     N  K  + + N + E    F           G   P P N
Sbjct:   19 LKDVLKSSISKINNVDEFIKFFFKDFKCTDELKKLDEEVLYVNLAASKAISQLAWNAY-QNNGLY-KDCHINA--------QYLEKIYFIMGNCNLYTNYLDKFKLGTEFLENVLIEMLSRYIIIHCILYYKEI-----DNLMFKSCIYCDCVVIGDICSVCEGNSFVLKDKSGDPMTFDFPFITDNVTDLKNQDLSSSSSNPTDCLESVS----DSIKTKDNDELNSTINISDYTEKIVSDSAIELNHKSRNQEVFHGSNLTS----FNDSFGLPEDKNQASKEYKDLNSSENISKLSPDFDLATEIEQTKETFWECKSCSFNKNKEHMQFCSVCLFDKQSQIDSENKNMDKISVTEDKILIEKENFNIEGNSDK------WSCNQCSFKNDKTDLLCSLCYANNPYENISHDVEDTWNCSKCKMKNPFDRYRCHQCDGLAGFFDKWVCEKCSFFNEKLDRNCYVCDLVNSNLIKCKKCEKNDVIEQDNEFCTSCIKENDYRGYGTPTPVN 501
[Close]
BLAST of SMED30010654 vs. Planmine SMEST
Match: SMESG000022823.1 (SMESG000022823.1)

HSP 1 Score: 59.3066 bits (142), Expect = 1.065e-8
Identity = 124/550 (22.55%), Postives = 218/550 (39.64%), Query Frame = 3 
Query:  363 FKLSKDQRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNM------PFDKYMEKIPKMNDSYIDESQLVEIIAIYVIIYSLLLNQEKILDKDDNM-------VCIYCDNLL--GDGICEVCDDTRD---------YSTKKNISFEKPDKKKEKSQNL--------------QTYNFESKQTIEEIDWENMDKI---------TKSNVKENKRN-GKASKAYNIKYTGEMKRDQPSVKTTDYY---SLNETTCEDQRADGFGNKSN--KTISAKTVIASNT-------NLSTLANTKSIVCDNKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTHENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKSRSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQANKISEMKTLFEP-GENAPVPNNASRIDNDNTWQC--TQCSYVNEKSTSVCEIC-FNIDRNPGKEAEGIPEETWRCNKCGNNNSFD 1820
             ++S D+ Y N+  D+LY  L N ++ AQ+ LN+Y+      ++KC   ++  ++      PF   +E + ++N   I E+ + E +  + +IY   L  E  LDK+  +       +C +C+  L  G   C VC+  +D         Y T   +  ++P K     QN+                Y+++     E+  +EN+++I         +  N+K N+ N  K SK +      E K  +  +   D +    LNE    +Q+   +G + N  K+ +  +V   NT       N+S++ + K ++C N+  C+               C     N     E+    L E+      + +AST N      + + W C +C   N           Y D      N+ R+ KL         W+C +C  +N      C +CG T  N +   + ++ KI+  +   +    NAP             W C   +CSY+N+   + C IC F   +      + I    W C KC   N ++
Sbjct:    4 LQISIDE-YNNYSTDNLYLELRNCRKKAQILLNLYHCPKE--YEKCKLLDIGSHLNNTNNKPFG--IENVQEINSDIIVEA-IGEFVKYHHVIYERELQVE--LDKNKEITDLLIGSICCFCEMRLDRGQSYCNVCEALKDDMTKYATNHYETLMLVKTQQPKKTSFIYQNVDKVPNFDPITHLNGANYDYKITYKPEKNSYENINEIQNAANRKEKSTENLKINEENIVKTSKDF-----SEWKCSKCGLLNYDEFCMACLNEKVITNQK---YGQEINLEKSGAKNSVEKRNTTSTYRYENVSSIESEKCVICRNETNCN---------------CKSNTKNSAITNENHKETLVENWE----NNDASTTN-----INSDQWKCDKCGMLN-----------YDDFCSACLNERRTIKL---------WKCERCKKLNDFFIEKCFSCGKTNENIQLCKQCKSIKIAADRRFCDTCYNNAP------------HWSCWNEKCSYLNDPFNNYCAICKFQRHKKDIFSIKDISGMFWLCKKCSAINDYE 481
[Close]
BLAST of SMED30010654 vs. Planmine SMEST
Match: SMESG000022826.1 (SMESG000022826.1)

HSP 1 Score: 58.151 bits (139), Expect = 2.238e-8
Identity = 59/253 (23.32%), Postives = 89/253 (35.18%), Query Frame = 3 
Query: 1851 AEFADKWVCGQCSFYNDKLSPRCGVCDMDNPRL-------------------IKCRKCRKGFVNAPNNQNND-ICGACKVSQK-------TVYNSLPSNNAIKSSPKPGSGD---------XXXXXXXXXXXXXLSLQNANVTQLAPSSGSGDTKRKSCKNCRKEFQPNSNNNWCITCEQMKWFCLNESCDSVN-FKVEFCKKCQVKKGSDLSNYKAFPISLQGKEVLSYQCGYCYQIIRSTINSCNSCKNPR 2498
            A+  D W+C QCSF ND     CG C + N  L                   I      + FV A     N  +C  C    K       T   S+  N  I S   P S +          +      K+     +Q+ N      +  S     ++C  C+     N     C+ C ++   C NE+C  ++     +C KC+V+K SD+ N   F +         +QC +C++        C  C N R
Sbjct:  249 AQSRDSWICNQCSFVNDSDLKNCGTCFLSNDLLKENSSSLTSPSDKSENENEIGLENSTENFVEAAKTVYNYWVCPNCTFKNKSDCLNCETCLKSIDINTQIISEYLPDSIENPIENLPKLFQNENLYEKSIKIPDVQDLNTPIEKKTGNSSKGSLRNCLKCKTLIDKNQQEFLCVDCSKLYLVCCNEACTQLSKADKSYCSKCKVRKNSDMKNGNLFDVKSMFSYEKKWQCSFCFRFNDKFQKKCRKCHNSR 501
The following BLAST results are available for this feature:
BLAST of SMED30010654 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
FO834800.18.485e-625.00E3 ubiquitin-protein ligase RNF31 [Source:NCBI gen... [more]
back to top
BLAST of SMED30010654 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rnf312.426e-727.16ring finger protein 31 [Source:NCBI gene;Acc:10003... [more]
rnf312.534e-727.16ring finger protein 31 [Source:NCBI gene;Acc:10003... [more]
rnf312.655e-727.16ring finger protein 31 [Source:NCBI gene;Acc:10003... [more]
rnf312.660e-727.16ring finger protein 31 [Source:NCBI gene;Acc:10003... [more]
rnf312.692e-727.16ring finger protein 31 [Source:NCBI gene;Acc:10003... [more]
back to top
BLAST of SMED30010654 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 1
Match NameE-valueIdentityDescription
Ranbp24.088e-623.26RAN binding protein 2 [Source:MGI Symbol;Acc:MGI:8... [more]
back to top
BLAST of SMED30010654 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1X7UH103.739e-1025.42Uncharacterized protein OS=Amphimedon queenslandic... [more]
A0A1A8H6N39.036e-1021.47RANBP2-like and GRIP domain containing 2 (Fragment... [more]
A0A1A8ETE81.294e-921.47RANBP2-like and GRIP domain containing 2 (Fragment... [more]
A0A1X7SKP52.604e-925.00Uncharacterized protein OS=Amphimedon queenslandic... [more]
A0A0N0P2D51.268e-824.00Uncharacterized protein OS=Leptomonas seymouri OX=... [more]
back to top
BLAST of SMED30010654 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
back to top
BLAST of SMED30010654 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 3
Match NameE-valueIdentityDescription
si:ch1073-55a19.23.664e-820.80RANBP2-like and GRIP domain-containing protein 8 [... [more]
si:ch1073-55a19.23.693e-820.80RANBP2-like and GRIP domain-containing protein 8 [... [more]
zranb1a8.997e-625.37zinc finger RANBP2-type containing 1 [Source:NCBI ... [more]
back to top
BLAST of SMED30010654 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000022828.10.000e+087.49SMESG000022828.1[more]
SMESG000022832.10.000e+090.44SMESG000022832.1[more]
SMESG000022833.11.494e-2930.53SMESG000022833.1[more]
SMESG000022823.11.065e-822.55SMESG000022823.1[more]
SMESG000022826.12.238e-823.32SMESG000022826.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010654 ID=SMED30010654|Name=SMED30010654|organism=Schmidtea mediterranea sexual|type=transcript|length=2801bp
GGATCTTAAATGCTTTATTTATATTAGAAGAATGAAATTCTTGTTTATGA
ATACTATTATATGTTAATTATATATATATACTGATAACTTAATAAAAATA
TTTATCAATTAAATTATACGTTCATAAAATTTACTTACCTTACAAATTAC
GATGCTTGCAATTTTAATATACCGTACTTAAAATATAAATTAAAAATTAT
TTGATTCAGTCAGTATATGTCTTTTGAGAGATGAATGGTCAAAATTTTGA
ATCTTTAGTAGAATGTATTGAAACAGATGAAAATACTGAACCGCTTAAAG
AGAAAATACGACAAAAAATTAGTTGTGTTAATCAAATCGACTATTTTCCT
GTAGAAGTTCAATTCAAATTGTCGAAAGACCAGAGATACCAGAACTTTGA
TATGGATGATCTCTATAAATATTTGATGAACACCAAACGAATCGCACAAT
TGTATTTGAATGTTTACAATAAGAATAATAGTTTATACTTTAAGAAATGT
CTTGCAAATGAGGTTAAAATGAATATGCCTTTCGATAAGTATATGGAAAA
GATACCGAAAATGAATGATAGTTATATCGATGAAAGTCAACTTGTAGAAA
TTATTGCAATTTATGTTATCATTTATTCATTATTATTGAATCAGGAAAAA
ATACTTGATAAAGATGACAATATGGTATGTATTTATTGTGACAACCTTCT
GGGTGATGGAATATGTGAAGTTTGTGACGACACCAGAGATTATTCAACAA
AGAAAAATATTTCATTTGAAAAACCTGACAAAAAGAAAGAAAAAAGTCAA
AATTTACAAACCTACAATTTTGAATCAAAGCAAACCATTGAAGAAATTGA
TTGGGAAAATATGGATAAGATAACTAAATCCAATGTTAAGGAAAATAAGA
GAAACGGAAAAGCATCAAAAGCATATAATATAAAATATACAGGTGAAATG
AAAAGAGATCAACCATCCGTTAAAACAACCGACTATTACAGTTTAAATGA
GACGACATGTGAGGATCAGAGAGCTGATGGATTCGGTAACAAATCAAATA
AAACAATTAGTGCGAAAACTGTCATTGCATCCAATACAAATCTGTCCACA
CTCGCTAATACCAAAAGCATTGTTTGTGACAATAAATGGGCGTGCAGCAA
ATGTTCGTATGAAAACGAAACATCAGATGTTAAATGTGTAACATGTAGTC
ACACTGATAGTAATGGAGGAAAAGAGACTGAATCTAGATCTAATAAGTTA
CCGGAAAGCCAAAGTATCACTGTAACACATGAAAATGCTTCAACACTCAA
TAATACCAACAGAATTGAAGATGGTAATTATTGGTCATGCACGCAATGTT
CCTTTAAAAATAAAAAGTCAACTTTTGGTTGTGTAACATGTGGTTACATT
GATAGGAACAGAGGAAAGGAGAATAAATCACGATCTGATAAACTGTTAGA
AATGCAAAACTTTGTTGATTCTTGGCAATGCGCCAAGTGCTCCTATATAA
ATAAAACATCAGCAGTTACTTGCGAAACATGTGGTGACACTGCTAGGAAC
AAAAGAAAAGCGAATGAATTGCAAGCAAATAAAATATCAGAAATGAAAAC
TCTATTTGAACCTGGTGAAAATGCCCCGGTACCCAATAATGCCAGCAGAA
TTGACAATGACAATACTTGGCAATGTACCCAATGCTCCTATGTAAATGAG
AAATCAACCTCTGTGTGTGAAATATGTTTCAACATTGATAGAAATCCTGG
AAAAGAAGCTGAAGGAATACCAGAGGAAACTTGGCGTTGTAATAAATGTG
GAAATAACAACTCATTTGATGTATACAAATGTCAGAAATGTCGAGAGATT
GCGGAATTCGCTGATAAATGGGTGTGTGGACAATGCTCATTTTACAACGA
CAAGCTGTCACCTCGTTGCGGAGTTTGTGATATGGATAATCCTCGTTTAA
TAAAATGTAGGAAATGCAGGAAAGGTTTTGTAAATGCGCCTAATAATCAA
AATAATGACATTTGCGGTGCTTGCAAAGTTTCACAGAAAACTGTCTATAA
TTCTTTGCCTAGTAACAATGCCATCAAATCATCTCCGAAGCCAGGTTCTG
GTGACGCCAAGAAAAATATTAATAACGGTAAAGCTGGCAACAATTTGAGC
TTGCAAAATGCTAATGTTACACAACTGGCACCGAGTTCAGGTTCTGGTGA
CACCAAGAGAAAGTCGTGTAAAAATTGCAGAAAGGAGTTTCAGCCAAATA
GTAATAACAATTGGTGTATAACATGTGAACAGATGAAATGGTTTTGTTTG
AATGAATCGTGTGACTCGGTTAATTTCAAGGTGGAATTTTGCAAGAAATG
CCAAGTCAAAAAAGGTTCTGATTTATCAAATTATAAAGCATTTCCTATTT
CATTACAAGGCAAAGAAGTCCTCAGCTATCAATGCGGATATTGTTACCAG
ATTATCCGCAGTACAATAAATTCCTGTAATTCCTGTAAAAATCCAAGGTA
TTTATCCAATCTCGTCCAATGCAAATGCGGCTGGAATTATAAAAAAATTG
AAAATGATTGTGGAAAATGTAAGGAAAAATTTTCCGAAAAATTATGAATT
TTCACTAATTTTTTCTGATGTTTTATTGTTAATGTCAGTGCTTGTTTCCC
ATGATAAATAATATTAATTGCCATCTTGATAAATTTTCCTGGAGTTTTCC
TATTTGTTCTTGAAAAATCCTGAAAGAACATTTTTGATATTTTGAGGTTA
GGTTACTCGACTTTTGGTCGCTATATATTGATAAAATAAATTTTATACCG
A
back to top

protein sequence of SMED30010654-orf-1

>SMED30010654-orf-1 ID=SMED30010654-orf-1|Name=SMED30010654-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=789bp
MNGQNFESLVECIETDENTEPLKEKIRQKISCVNQIDYFPVEVQFKLSKD
QRYQNFDMDDLYKYLMNTKRIAQLYLNVYNKNNSLYFKKCLANEVKMNMP
FDKYMEKIPKMNDSYIDESQLVEIIAIYVIIYSLLLNQEKILDKDDNMVC
IYCDNLLGDGICEVCDDTRDYSTKKNISFEKPDKKKEKSQNLQTYNFESK
QTIEEIDWENMDKITKSNVKENKRNGKASKAYNIKYTGEMKRDQPSVKTT
DYYSLNETTCEDQRADGFGNKSNKTISAKTVIASNTNLSTLANTKSIVCD
NKWACSKCSYENETSDVKCVTCSHTDSNGGKETESRSNKLPESQSITVTH
ENASTLNNTNRIEDGNYWSCTQCSFKNKKSTFGCVTCGYIDRNRGKENKS
RSDKLLEMQNFVDSWQCAKCSYINKTSAVTCETCGDTARNKRKANELQAN
KISEMKTLFEPGENAPVPNNASRIDNDNTWQCTQCSYVNEKSTSVCEICF
NIDRNPGKEAEGIPEETWRCNKCGNNNSFDVYKCQKCREIAEFADKWVCG
QCSFYNDKLSPRCGVCDMDNPRLIKCRKCRKGFVNAPNNQNNDICGACKV
SQKTVYNSLPSNNAIKSSPKPGSGDAKKNINNGKAGNNLSLQNANVTQLA
PSSGSGDTKRKSCKNCRKEFQPNSNNNWCITCEQMKWFCLNESCDSVNFK
VEFCKKCQVKKGSDLSNYKAFPISLQGKEVLSYQCGYCYQIIRSTINSCN
SCKNPRYLSNLVQCKCGWNYKKIENDCGKCKEKFSEKL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000034epidermis
PLANA:0000070intestinal phagocyte
PLANA:0000074oocyte
PLANA:0000099neuron
PLANA:0000231vitelline gland
PLANA:0002032epidermal cell
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR001876Znf_RanBP2
Vocabulary: molecular function
TermDefinition
GO:0008270zinc ion binding
GO:0046872metal ion binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001876Zinc finger, RanBP2-typeSMARTSM00547zf_4coord: 366..390
e-value: 0.3
score: 16.0
coord: 545..569
e-value: 1.1
score: 11.4
coord: 516..540
e-value: 2.3
score: 9.1
coord: 478..502
e-value: 0.06
score: 21.6
coord: 301..325
e-value: 0.0049
score: 26.1
coord: 413..437
e-value: 0.0039
score: 26.4
IPR001876Zinc finger, RanBP2-typePFAMPF00641zf-RanBPcoord: 302..323
e-value: 0.0019
score: 17.5
coord: 412..435
e-value: 9.3E-5
score: 21.7
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 415..434
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 303..322
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 480..499
IPR001876Zinc finger, RanBP2-typePROSITEPS01358ZF_RANBP2_1coord: 547..566
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 411..440
score: 8.321
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 299..328
score: 8.761
IPR001876Zinc finger, RanBP2-typePROSITEPS50199ZF_RANBP2_2coord: 363..393
score: 8.75