max-like protein x

Overview


Name	max-like protein x (AKG62113.1)
Smed ID	SMED30010649
Length (bp)	1205

Neoblast Clusters

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.

Expression of max-like protein x (SMED30010649) t-SNE clustered cells

Violin plots show distribution of expression levels for max-like protein x (SMED30010649) in cells (dots) of each of the 12 neoblast clusters.

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of max-like protein x (SMED30010649) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for max-like protein x (SMED30010649) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

Anatomical Expression

PAGE et. al., 2020

SMED30010649

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Expressed In	Reference Transcript	Gene Models	Published Transcript	Transcriptome	Publication
nervous system	SMED30010649	SMESG000025391.1	dd_Smed_v4_3140_0_1	dd_Smed_v4	PMID:29674431 Fincher et al., 2018
pharynx	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013
gut	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013
central nervous system	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013
progenitor cell	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013
stem cell	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013
parenchyma	SMED30010649	SMESG000025391.1	HO006087	ncbi_smed_ests	PMID:24173799 Cowles et al., 2013

Note: Hover over icons to view figure legend

Homology

[Close]

BLAST of max-like protein x vs. Ensembl Human
Match: MLX (MAX dimerization protein MLX [Source:HGNC Symbol;Acc:HGNC:11645])

HSP 1 Score: 104.375 bits (259), Expect = 1.757e-25
Identity = 69/165 (41.82%), Postives = 103/165 (62.42%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+CKPQ L  +V  VL
Sbjct:   43 KDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVL 206

[Close]

BLAST of max-like protein x vs. Ensembl Human
Match: MLX (MAX dimerization protein MLX [Source:HGNC Symbol;Acc:HGNC:11645])

HSP 1 Score: 104.76 bits (260), Expect = 2.165e-25
Identity = 77/189 (40.74%), Postives = 114/189 (60.32%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV + + DED D      +   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+CKPQ L  +V  VL
Sbjct:   50 ASSVPNTD-DEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVL 236

[Close]

BLAST of max-like protein x vs. Ensembl Human
Match: MLX (MAX dimerization protein MLX [Source:HGNC Symbol;Acc:HGNC:11645])

HSP 1 Score: 105.531 bits (262), Expect = 4.141e-25
Identity = 77/189 (40.74%), Postives = 114/189 (60.32%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV + + DED D      +   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+CKPQ L  +V  VL
Sbjct:  104 ASSVPNTD-DEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVL 290

[Close]

BLAST of max-like protein x vs. Ensembl Human
Match: MLX (MAX dimerization protein MLX [Source:HGNC Symbol;Acc:HGNC:11645])

HSP 1 Score: 94.3597 bits (233), Expect = 1.690e-21
Identity = 67/168 (39.88%), Postives = 101/168 (60.12%), Query Frame = 2

Query:  188 DEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCK 670
            DED D      +   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+CK
Sbjct:   82 DEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCK 248

[Close]

BLAST of max-like protein x vs. Ensembl Celegans
Match: mml-1 (pep chromosome:WBcel235:III:7370131:7374731:-1 gene:WBGene00003378.1 transcript:T20B12.6a.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mml-1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.603e-6
Identity = 24/57 (42.11%), Postives = 35/57 (61.40%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSL 394
            ++R+R LH  AEQ RR A+K G+D LM +I P   + G K + A +L ++   IR L
Sbjct:  801 EERKRILHLHAEQNRRSALKDGFDQLMDII-PDLYSGGVKPTNAVVLAKSADHIRRL 856

[Close]

BLAST of max-like protein x vs. Ensembl Celegans
Match: mml-1 (pep chromosome:WBcel235:III:7370149:7374731:-1 gene:WBGene00003378.1 transcript:T20B12.6b.1 gene_biotype:protein_coding transcript_biotype:protein_coding gene_symbol:mml-1)

HSP 1 Score: 49.6766 bits (117), Expect = 3.670e-6
Identity = 24/57 (42.11%), Postives = 35/57 (61.40%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSL 394
            ++R+R LH  AEQ RR A+K G+D LM +I P   + G K + A +L ++   IR L
Sbjct:  801 EERKRILHLHAEQNRRSALKDGFDQLMDII-PDLYSGGVKPTNAVVLAKSADHIRRL 856

[Close]

BLAST of max-like protein x vs. Ensembl Fly
Match: bigmax (gene:FBgn0039509 transcript:FBtr0085173)

HSP 1 Score: 97.8265 bits (242), Expect = 3.977e-23
Identity = 69/164 (42.07%), Postives = 102/164 (62.20%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETN---GTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            K+RRRE HTQAEQ+RRDAIK GYDSL  L+ P  + N   G KLSKA ILQ++I  I  L++Q   +E E   L+ E+ ALRI+ + YE M +  +     EE   + ++ KF++F   ++++F++F +    +NF   +T II W+EE+CKP  L  ++   L
Sbjct:   69 KERRREAHTQAEQKRRDAIKKGYDSLQELV-PRCQPNDSSGYKLSKALILQKSIEYIGYLNQQKLKQEDEGSALQKEVTALRIIKNGYENMLQHQQANPGPEEAR-LTDEAKFQVFQAIMEEMFETF-QHIPMENFKQLTTGIIPWLEEHCKPHILRNILSRTL 229

[Close]

BLAST of max-like protein x vs. Ensembl Fly
Match: Mondo (gene:FBgn0032940 transcript:FBtr0081527)

HSP 1 Score: 48.9062 bits (115), Expect = 7.110e-6
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 2

Query:  221 RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSI 412
            R  +RR  H  AEQ+RR  IK+G+D+L  LI         KLSKA +LQ+    I+ L ++ ++
Sbjct:  714 RDTQRRAGHIHAEQKRRYNIKNGFDTLHALIPQLQLNPNAKLSKAAMLQKGADHIKQLRQERNV 777

[Close]

BLAST of max-like protein x vs. Ensembl Fly
Match: Mondo (gene:FBgn0032940 transcript:FBtr0301458)

HSP 1 Score: 48.9062 bits (115), Expect = 7.522e-6
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 2

Query:  221 RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSI 412
            R  +RR  H  AEQ+RR  IK+G+D+L  LI         KLSKA +LQ+    I+ L ++ ++
Sbjct:  908 RDTQRRAGHIHAEQKRRYNIKNGFDTLHALIPQLQLNPNAKLSKAAMLQKGADHIKQLRQERNV 971

[Close]

BLAST of max-like protein x vs. Ensembl Fly
Match: Mondo (gene:FBgn0032940 transcript:FBtr0301457)

HSP 1 Score: 48.9062 bits (115), Expect = 7.522e-6
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 2

Query:  221 RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSI 412
            R  +RR  H  AEQ+RR  IK+G+D+L  LI         KLSKA +LQ+    I+ L ++ ++
Sbjct:  908 RDTQRRAGHIHAEQKRRYNIKNGFDTLHALIPQLQLNPNAKLSKAAMLQKGADHIKQLRQERNV 971

[Close]

BLAST of max-like protein x vs. Ensembl Fly
Match: Mondo (gene:FBgn0032940 transcript:FBtr0301462)

HSP 1 Score: 48.9062 bits (115), Expect = 7.522e-6
Identity = 25/64 (39.06%), Postives = 37/64 (57.81%), Query Frame = 2

Query:  221 RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSI 412
            R  +RR  H  AEQ+RR  IK+G+D+L  LI         KLSKA +LQ+    I+ L ++ ++
Sbjct:  908 RDTQRRAGHIHAEQKRRYNIKNGFDTLHALIPQLQLNPNAKLSKAAMLQKGADHIKQLRQERNV 971

[Close]

BLAST of max-like protein x vs. Ensembl Zebrafish
Match: mlx (MAX dimerization protein MLX [Source:ZFIN;Acc:ZDB-GENE-050522-316])

HSP 1 Score: 74.7146 bits (182), Expect = 6.596e-15
Identity = 59/163 (36.20%), Postives = 93/163 (57.06%), Query Frame = 2

Query:  245 HTQAEQRRRDAIKSGYDSLMPLIHPASETNG------TKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDV 715
            HT+ + R    IK GYD L  ++ P  +          K+SKAT+LQ+TI  I+ L ++   +E+++  L+ E+ AL+IM +NYE + K ++N  + + + +V +QVKF +F   +  LFQSF  S    +F   S  +  W+EE+CKPQ L   V +VL  V
Sbjct:   65 HTRRQSRNAGIIKKGYDDLQSIV-PTCQQQSDFSMATQKMSKATVLQKTIDYIQFLHKEKKKQEEDVSTLRKEVMALKIMKTNYEHIVKAHQNNPQ-QGSEQVSDQVKFSVFQSIMDSLFQSFSASVSVSSFQELSACVFSWIEEHCKPQTLREFVVTVLQQV 225

[Close]

BLAST of max-like protein x vs. Ensembl Xenopus
Match: MLX (MLX, MAX dimerization protein [Source:NCBI gene;Acc:101732928])

HSP 1 Score: 124.79 bits (312), Expect = 1.329e-32
Identity = 82/220 (37.27%), Postives = 133/220 (60.45%), Query Frame = 2

Query:   89 YSKALMYNVNNPSNMYDQSQIVKIASSVSSQNS-------DEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETN---GT-KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ++  +M+N+   S + ++  +V  A+S+ S ++       DED D     I+   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + +   GT KLSKA +LQ+TI  I+ L+++   +E ++  L+ E+ AL+IM SNYE++ K ++N +  E T++VP+++KF +F   +  LF +F+ S   ++F   S  +  W+EE+CKPQ L  +V   L
Sbjct:   34 HAGGVMFNLEVESGLINKGSLVSRANSIGSTSASSVPNTDDEDSDDRQETIKNDYKDRRRRAHTQAEQKRRDAIKKGYDDLQCIVPTCQQQDIAIGTQKLSKAVVLQKTIDYIQFLNKEKKKQEDDVSTLRKEVMALQIMKSNYEQIVKAHQNNLH-EGTDQVPDEMKFRVFQGIMDSLFHTFNSSVSVNSFQELSACVFAWIEEHCKPQTLQDIVIRFL 252

[Close]

BLAST of max-like protein x vs. Ensembl Xenopus
Match: MLX (MLX, MAX dimerization protein [Source:NCBI gene;Acc:101732928])

HSP 1 Score: 120.939 bits (302), Expect = 1.134e-31
Identity = 81/205 (39.51%), Postives = 125/205 (60.98%), Query Frame = 2

Query:  122 PSNMYDQSQIVKIASSVSSQNS-------DEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETN---GT-KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLV 694
            PSN   +  +V  A+S+ S ++       DED D     I+   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + +   GT KLSKA +LQ+TI  I+ L+++   +E ++  L+ E+ AL+IM SNYE++ K ++N +  E T++VP+++KF +F   +  LF +F+ S   ++F   S  +  W+EE+CKPQ L  +V
Sbjct:    2 PSNWLQKGSLVSRANSIGSTSASSVPNTDDEDSDDRQETIKNDYKDRRRRAHTQAEQKRRDAIKKGYDDLQCIVPTCQQQDIAIGTQKLSKAVVLQKTIDYIQFLNKEKKKQEDDVSTLRKEVMALQIMKSNYEQIVKAHQNNLH-EGTDQVPDEMKFRVFQGIMDSLFHTFNSSVSVNSFQELSACVFAWIEEHCKPQTLQDIV 205

[Close]

BLAST of max-like protein x vs. Ensembl Xenopus
Match: MLX (MLX, MAX dimerization protein [Source:NCBI gene;Acc:101732928])

HSP 1 Score: 114.005 bits (284), Expect = 5.387e-29
Identity = 76/179 (42.46%), Postives = 115/179 (64.25%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETN---GT-KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQ 676
            ASSV + + DED D     I+   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + +   GT KLSKA +LQ+TI  I+ L+++   +E ++  L+ E+ AL+IM SNYE++ K ++N +  E T++VP+++KF +F   +  LF +F+ S   ++F   S  +  W+EE+CKPQ
Sbjct:   19 ASSVPNTD-DEDSDDRQETIKNDYKDRRRRAHTQAEQKRRDAIKKGYDDLQCIVPTCQQQDIAIGTQKLSKAVVLQKTIDYIQFLNKEKKKQEDDVSTLRKEVMALQIMKSNYEQIVKAHQNNLH-EGTDQVPDEMKFRVFQGIMDSLFHTFNSSVSVNSFQELSACVFAWIEEHCKPQ 195

[Close]

BLAST of max-like protein x vs. Ensembl Mouse
Match: Mlx (MAX-like protein X [Source:MGI Symbol;Acc:MGI:108398])

HSP 1 Score: 102.834 bits (255), Expect = 4.198e-25
Identity = 68/165 (41.21%), Postives = 103/165 (62.42%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+C+PQ L  +V  VL
Sbjct:   43 KDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQTLREIVIGVL 206

[Close]

BLAST of max-like protein x vs. Ensembl Mouse
Match: Mlx (MAX-like protein X [Source:MGI Symbol;Acc:MGI:108398])

HSP 1 Score: 103.219 bits (256), Expect = 6.886e-25
Identity = 68/165 (41.21%), Postives = 103/165 (62.42%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+C+PQ L  +V  VL
Sbjct:   73 KDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQTLREIVIGVL 236

[Close]

BLAST of max-like protein x vs. Ensembl Mouse
Match: Mlx (MAX-like protein X [Source:MGI Symbol;Acc:MGI:108398])

HSP 1 Score: 103.219 bits (256), Expect = 1.908e-24
Identity = 68/165 (41.21%), Postives = 103/165 (62.42%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+C+PQ L  +V  VL
Sbjct:  127 KDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQTLREIVIGVL 290

[Close]

BLAST of max-like protein x vs. Ensembl Mouse
Match: Mlx (MAX-like protein X [Source:MGI Symbol;Acc:MGI:108398])

HSP 1 Score: 49.2914 bits (116), Expect = 5.655e-7
Identity = 21/30 (70.00%), Postives = 24/30 (80.00%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLI 313
            KDRRR  HTQAEQ+RRDAIK GYD L  ++
Sbjct:   97 KDRRRRAHTQAEQKRRDAIKRGYDDLQTIV 126

[Close]

BLAST of max-like protein x vs. UniProt/SwissProt
Match: sp|Q9UH92|MLX_HUMAN (Max-like protein X OS=Homo sapiens OX=9606 GN=MLX PE=1 SV=2)

HSP 1 Score: 105.531 bits (262), Expect = 1.988e-24
Identity = 77/189 (40.74%), Postives = 114/189 (60.32%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV + + DED D      +   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+CKPQ L  +V  VL
Sbjct:  104 ASSVPNTD-DEDSDYHQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCKPQTLREIVIGVL 290

[Close]

BLAST of max-like protein x vs. UniProt/SwissProt
Match: sp|O08609|MLX_MOUSE (Max-like protein X OS=Mus musculus OX=10090 GN=Mlx PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 9.142e-24
Identity = 76/189 (40.21%), Postives = 114/189 (60.32%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIR---KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGT----KLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV + + DED D      +   KDRRR  HTQAEQ+RRDAIK GYD L  ++    + + +    KLSKA +LQ+TI  I+ L ++   +E+E+  L+ ++ AL+IM  NYE++ K +++    E  ++V +QVKF +F   +  LFQSF+ S    +F   S  +  W+EE+C+PQ L  +V  VL
Sbjct:  104 ASSVPNTD-DEDSDYQQEAYKESYKDRRRRAHTQAEQKRRDAIKRGYDDLQTIVPTCQQQDFSIGSQKLSKAIVLQKTIDYIQFLHKEKKKQEEEVSTLRKDVTALKIMKVNYEQIVKAHQDN-PHEGEDQVSDQVKFNVFQGIMDSLFQSFNASISVASFQELSACVFSWIEEHCEPQTLREIVIGVL 290

[Close]

BLAST of max-like protein x vs. TrEMBL
Match: A0A0F7GXZ2 (Max-like protein x (Fragment) OS=Schmidtea mediterranea OX=79327 PE=2 SV=1)

HSP 1 Score: 455.677 bits (1171), Expect = 3.038e-158
Identity = 250/251 (99.60%), Postives = 250/251 (99.60%), Query Frame = 2

Query:  371 TISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE 1123
             ISQIRSLSEQTSIKEKELENLKNELFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIFGQFLQQLFQSFDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE
Sbjct:   17 AISQIRSLSEQTSIKEKELENLKNELFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIFGQFLQQLFQSFDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE 267

[Close]

BLAST of max-like protein x vs. TrEMBL
Match: A0A0P4VQ84 (Putative bhlhzip transcription factor bigmax (Fragment) OS=Rhodnius neglectus OX=72488 PE=2 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 6.600e-33
Identity = 90/181 (49.72%), Postives = 118/181 (65.19%), Query Frame = 2

Query:  188 DEDIDTANIMI-RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPA--SETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLS 721
            +ED D  N ++  KDRRRE HTQAEQ+RRDAIK GYDSL  L+     S+ +G KLSKAT+LQ++I  I+ L +Q   +E E   L+ E+ ALRIM +NYE+M K  +NQ    E+  V ++VKF++F   L QLF +F+ S   +NFS  S  I  W+EEYCKPQ L  +V SVL  V S
Sbjct:    2 EEDGDDKNCIVPYKDRRREAHTQAEQKRRDAIKKGYDSLQSLLPTCQQSDISGYKLSKATVLQKSIDYIQFLLQQKKKEEDERNALRKEVIALRIMQANYEQMVKAQENQPGLIESR-VCDEVKFDVFQNILDQLFITFN-SVSVNNFSELSACIFTWLEEYCKPQKLREMVTSVLQRVYS 180

[Close]

BLAST of max-like protein x vs. TrEMBL
Match: T1HED9 (BHLH domain-containing protein (Fragment) OS=Rhodnius prolixus OX=13249 PE=4 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 3.658e-32
Identity = 84/182 (46.15%), Postives = 111/182 (60.99%), Query Frame = 2

Query:  188 DEDIDTANIMI-RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPA--SETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLSN 724
            +ED D  N ++  KDRRRE HTQAEQ+RRDAIK GYDSL  L+     S+ +G KLSKAT+LQ++I  I+ L +Q   +E E   L+ E+ ALRIM  NYE+M K  +NQ    E+  V ++VKF++       +      S   +NFS  S  I  W+EEYCKPQ L  +V SVL  V S+
Sbjct:    1 EEDGDDKNCIVPYKDRRREAHTQAEQKRRDAIKKGYDSLQSLLPTCQQSDISGYKLSKATVLQKSIDYIQFLLQQKKKEEDERNALRKEVIALRIMQVNYEQMVKAQENQPGLIESR-VCDEVKFDVVCILPLFITF---NSVSVNNFSELSACIFTWLEEYCKPQKLREMVTSVLQRVYSS 178

[Close]

BLAST of max-like protein x vs. TrEMBL
Match: A0A224XYZ1 (Putative bhlhzip transcription factor bigmax (Fragment) OS=Panstrongylus lignarius OX=156445 PE=4 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 1.914e-31
Identity = 88/181 (48.62%), Postives = 117/181 (64.64%), Query Frame = 2

Query:  188 DEDIDTANIMI-RKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPA--SETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLS 721
            +ED D  N ++  KDRRRE HTQAEQ+RRD IK GYDSL  L+     S+ +G KLSKAT+LQ++I  I+ L +Q   +E E   L+ E+ ALRIM +NYE+M K  +NQ    E+  V ++VKF++F   L QLF +F+ S   +NFS  S  I  W+EEYCKPQ L  +V +VL  V S
Sbjct:    2 EEDGDDKNCIVPYKDRRREAHTQAEQKRRDNIKKGYDSLQSLLPTCQQSDISGYKLSKATVLQKSIDYIQFLLQQKKKEEDERNALRKEVIALRIMQANYEQMVKAQENQPGLIESR-VCDEVKFDVFQNILDQLFITFN-SVSVNNFSELSACIFTWLEEYCKPQKLREMVTTVLQRVYS 180

[Close]

BLAST of max-like protein x vs. TrEMBL
Match: A0A2R5L8J0 (Putative bhlhzip transcription factor bigmax (Fragment) OS=Ornithodoros turicata OX=34597 PE=4 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 2.201e-31
Identity = 85/192 (44.27%), Postives = 121/192 (63.02%), Query Frame = 2

Query:  149 IVKIASSVSSQNSD-EDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASE--TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDV 715
            I  I +S S+ N+D ED D  + +  K+RRRE HTQAEQ+RRDAIK GY+ L  L+    +  TN  K+SKA +LQ++I  I  L +Q    E+EL  L+ E+ AL+IM +NYE++ K N+NQ   +  N+V +++KFE+F      LFQSF+ S    NFS  S  +  W+EEYCKPQ L  +V  +L+ +
Sbjct:   37 IDNILTSSSAHNTDDEDSDHKSTLSYKERRREAHTQAEQKRRDAIKKGYEDLQFLVPTCQQDSTNAVKMSKAAVLQKSIDYITFLLQQKKKHEEELNALRKEVVALQIMKTNYEQLVKVNQNQ-PGQNKNQVSDEMKFEVFQAIGDSLFQSFNASVSVSNFSELSGCVFGWLEEYCKPQTLRDMVLQILSQL 227

[Close]

BLAST of max-like protein x vs. Ensembl Cavefish
Match: mlx (MAX dimerization protein MLX [Source:NCBI gene;Acc:103046275])

HSP 1 Score: 103.605 bits (257), Expect = 3.455e-25
Identity = 81/190 (42.63%), Postives = 115/190 (60.53%), Query Frame = 2

Query:  161 ASSV---SSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASE--TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV     ++SD   +TA     KDRRR+ HTQAEQ+RRDAIK GYD   S++P     SE      K+SKAT+LQ+TI  I+ L ++   +E+E+  L+ E+ AL+IM +NYE + K ++N  + + + +V +QVKF +F   +  LFQSF  S    +F   S  +  W+EE+CKPQ L   V  VL
Sbjct:   52 ASSVPNTDDEDSDYRHETAYKDSYKDRRRQAHTQAEQKRRDAIKKGYDDLQSIVPTCQQQSEFAMATQKISKATVLQKTIDYIQFLHKEKKKQEEEVSTLRKEVMALKIMKTNYEHIVKAHQNNPQ-QGSEQVSDQVKFSVFQNIMDSLFQSFSTSVSVSSFQELSACVFSWIEEHCKPQTLREFVVGVL 240

[Close]

BLAST of max-like protein x vs. Ensembl Cavefish
Match: mlx (MAX dimerization protein MLX [Source:NCBI gene;Acc:103046275])

HSP 1 Score: 103.605 bits (257), Expect = 3.556e-25
Identity = 81/190 (42.63%), Postives = 115/190 (60.53%), Query Frame = 2

Query:  161 ASSV---SSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASE--TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV     ++SD   +TA     KDRRR+ HTQAEQ+RRDAIK GYD   S++P     SE      K+SKAT+LQ+TI  I+ L ++   +E+E+  L+ E+ AL+IM +NYE + K ++N  + + + +V +QVKF +F   +  LFQSF  S    +F   S  +  W+EE+CKPQ L   V  VL
Sbjct:   51 ASSVPNTDDEDSDYRHETAYKDSYKDRRRQAHTQAEQKRRDAIKKGYDDLQSIVPTCQQQSEFAMATQKISKATVLQKTIDYIQFLHKEKKKQEEEVSTLRKEVMALKIMKTNYEHIVKAHQNNPQ-QGSEQVSDQVKFSVFQNIMDSLFQSFSTSVSVSSFQELSACVFSWIEEHCKPQTLREFVVGVL 239

[Close]

BLAST of max-like protein x vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005581.1 (pep scaffold:Pmarinus_7.0:GL487226:4176:7420:1 gene:ENSPMAG00000005067.1 transcript:ENSPMAT00000005581.1 gene_biotype:protein_coding transcript_biotype:protein_coding)

HSP 1 Score: 109.768 bits (273), Expect = 4.301e-28
Identity = 77/185 (41.62%), Postives = 114/185 (61.62%), Query Frame = 2

Query:  161 ASSVSSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASE---TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVL 706
            ASSV + + DED D    +  KDRRR+ HTQAEQ+RRDAIK GY+ L  ++    +       K+SKAT+LQR+I  I  L ++   +E+EL  L+ E+ AL+IM SNYE++ + +++  + +   +V ++VKF IF   +  LFQSF+ S    +F   S  +  W+EE+CKPQ L  +V  VL
Sbjct:   56 ASSVPNTD-DEDSDYRQELTYKDRRRQAHTQAEQKRRDAIKKGYEDLQAIVPTCMQQDLIGSQKMSKATVLQRSIDYIHFLHKEKKKQEEELSTLRKEVLALKIMKSNYEQIVRAHQSNPQ-QGQEQVSDEVKFSIFQGIMDTLFQSFNASIAVSSFQELSACVFSWLEEHCKPQTLREIVLGVL 238

[Close]

BLAST of max-like protein x vs. Ensembl Sea Lamprey
Match: MLXIPL (MLX interacting protein like [Source:HGNC Symbol;Acc:HGNC:12744])

HSP 1 Score: 48.521 bits (114), Expect = 2.052e-6
Identity = 25/60 (41.67%), Postives = 37/60 (61.67%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQ 403
            +D RR  H  AEQ+RR  IK G+D+L  L+ P +    +K+SKA  LQ+T+  I  L ++
Sbjct:  609 EDSRRVTHISAEQKRRFNIKIGFDTLYNLVSPLNLQPNSKVSKAATLQKTVEYITKLQQE 668

[Close]

BLAST of max-like protein x vs. Ensembl Nematostella
Match: EDO46174 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RQW2])

HSP 1 Score: 107.842 bits (268), Expect = 1.655e-27
Identity = 77/201 (38.31%), Postives = 120/201 (59.70%), Query Frame = 2

Query:  134 YDQSQIVKIASSVSSQ-NSDED----IDTANIMIRKDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQN-LNVLVESVLT 709
            ++ ++IV ++S +  Q NSD D    +D  +    K  RR  H+ AEQ+RRDAIK GYD   S++P    ++     KLSKA ILQRTI  +  +  Q    E+ELE L+ E+ AL+IM  NYE++A+ + +Q  + +  +V E+ KF +F   L   F +FD +   ++F + S+ I HW+EEYCKPQ  ++ ++ S+ T
Sbjct:    5 WNDTEIVSLSSEIDDQQNSDSDALDELDFDSSSSSKQNRRFAHSVAEQKRRDAIKKGYDDLQSIVPTCQHSTSAGSPKLSKAIILQRTIEYVSFMHHQRKKHEEELEKLRKEVLALKIMKQNYEQIARAHADQPMSGQ--QVSEEAKFSVFKAILDNQFVTFDGTVVVNDFDSLSSGIFHWLEEYCKPQYFMDTVIRSLRT 203

[Close]

BLAST of max-like protein x vs. Ensembl Nematostella
Match: EDO27547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T8Y7])

HSP 1 Score: 102.064 bits (253), Expect = 5.779e-26
Identity = 66/154 (42.86%), Postives = 93/154 (60.39%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQ 676
            K  RR  H+ AEQ+RRDAIK GYD   S++P    ++     KLSKA ILQRTI  +  +  Q    E+ELE L+ E+ AL+IM  NYE++A+ + +Q       +V E+ KF +F   L   F +FD +   ++F + S+ I HW+EEYCKPQ
Sbjct:   12 KQNRRFAHSVAEQKRRDAIKKGYDDLQSIVPTCQHSTSAGSPKLSKAIILQRTIEYVSFMHHQRKKHEEELEKLRKEVLALKIMKQNYEQIARAHADQ--PMPGQQVSEEAKFSVFKAILDNQFVTFDGTVVVNDFDSLSSGIFHWLEEYCKPQ 163

[Close]

BLAST of max-like protein x vs. Ensembl Nematostella
Match: EDO39391 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SA35])

HSP 1 Score: 45.4394 bits (106), Expect = 9.812e-6
Identity = 21/50 (42.00%), Postives = 32/50 (64.00%), Query Frame = 2

Query:  227 DRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTI 376
            + RR  H  AEQ+RR  IK G+D L  ++   +    +K+SKAT+LQ+T+
Sbjct:    1 EHRRMSHISAEQKRRCNIKMGFDQLASMVPTLASQKSSKVSKATVLQKTV 50

[Close]

BLAST of max-like protein x vs. Ensembl Medaka
Match: mlx (MAX dimerization protein MLX [Source:NCBI gene;Acc:101167456])

HSP 1 Score: 106.301 bits (264), Expect = 4.536e-26
Identity = 74/169 (43.79%), Postives = 104/169 (61.54%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASE--TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDV 715
            KDRRR+ HTQAEQ+RRDAIK GYD   S++P     SE      K+SKATILQ+TI  I  L ++   +E+E+  L+ E+ AL+IM +NYE + K +++  +  E ++V +QVKF+IF   +  LF SF  S    +F   S  +  W+EE+CKPQ L   V  VL  +
Sbjct:   79 KDRRRQAHTQAEQKRRDAIKKGYDDLQSIVPTCQQQSEFAVGAQKISKATILQKTIDYIHFLHKEKKKQEEEVSVLRKEVMALKIMKTNYEHIVKAHQSNPQQGE-DQVSDQVKFDIFQSIMDSLFVSFSNSVSVSSFEELSACVFSWIEEHCKPQTLREFVVGVLRQI 246

[Close]

BLAST of max-like protein x vs. Ensembl Medaka
Match: mlx (MAX dimerization protein MLX [Source:NCBI gene;Acc:101167456])

HSP 1 Score: 105.916 bits (263), Expect = 5.604e-26
Identity = 74/169 (43.79%), Postives = 104/169 (61.54%), Query Frame = 2

Query:  224 KDRRRELHTQAEQRRRDAIKSGYD---SLMPLIHPASE--TNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDV 715
            KDRRR+ HTQAEQ+RRDAIK GYD   S++P     SE      K+SKATILQ+TI  I  L ++   +E+E+  L+ E+ AL+IM +NYE + K +++  +  E ++V +QVKF+IF   +  LF SF  S    +F   S  +  W+EE+CKPQ L   V  VL  +
Sbjct:   72 KDRRRQAHTQAEQKRRDAIKKGYDDLQSIVPTCQQQSEFAVGAQKISKATILQKTIDYIHFLHKEKKKQEEEVSVLRKEVMALKIMKTNYEHIVKAHQSNPQQGE-DQVSDQVKFDIFQSIMDSLFVSFSNSVSVSSFEELSACVFSWIEEHCKPQTLREFVVGVLRQI 239

[Close]

BLAST of max-like protein x vs. Planmine SMEST
Match: SMESG000025391.1 (SMESG000025391.1)

HSP 1 Score: 637.491 bits (1643), Expect = 0.000e+0
Identity = 339/340 (99.71%), Postives = 340/340 (100.00%), Query Frame = 2

Query:  104 MYNVNNPSNMYDQSQIVKIASSVSSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSIXXXXXXXXXXXXFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIXXXXXXXXXXXXDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTDVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE 1123
            MYNVNNPSNMYDQSQIVKIASSVSSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSIKEKELENLKNELFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIFGQFLQQLFQSFDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLT+VLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE
Sbjct:    1 MYNVNNPSNMYDQSQIVKIASSVSSQNSDEDIDTANIMIRKDRRRELHTQAEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQTSIKEKELENLKNELFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKFEIFGQFLQQLFQSFDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESVLTEVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSYRSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQSLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE 340

The following BLAST results are available for this feature:

BLAST of max-like protein x vs. Ensembl Human
BLAST of max-like protein x vs. Ensembl Celegans
BLAST of max-like protein x vs. Ensembl Fly
BLAST of max-like protein x vs. Ensembl Zebrafish
BLAST of max-like protein x vs. Ensembl Xenopus
BLAST of max-like protein x vs. Ensembl Mouse
BLAST of max-like protein x vs. UniProt/SwissProt
BLAST of max-like protein x vs. TrEMBL
BLAST of max-like protein x vs. Ensembl Cavefish
BLAST of max-like protein x vs. Ensembl Sea Lamprey
BLAST of max-like protein x vs. Ensembl Yeast
BLAST of max-like protein x vs. Ensembl Nematostella
BLAST of max-like protein x vs. Ensembl Medaka
BLAST of max-like protein x vs. Planmine SMEST

BLAST of max-like protein x vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 4


Match Name	E-value	Identity	Description
MLX	1.757e-25	41.82	MAX dimerization protein MLX [Source:HGNC Symbol;A...	[more]
MLX	2.165e-25	40.74	MAX dimerization protein MLX [Source:HGNC Symbol;A...	[more]
MLX	4.141e-25	40.74	MAX dimerization protein MLX [Source:HGNC Symbol;A...	[more]
MLX	1.690e-21	39.88	MAX dimerization protein MLX [Source:HGNC Symbol;A...	[more]

BLAST of max-like protein x vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mml-1	3.603e-6	42.11	pep chromosome:WBcel235:III:7370131:7374731:-1 gen...	[more]
mml-1	3.670e-6	42.11	pep chromosome:WBcel235:III:7370149:7374731:-1 gen...	[more]

BLAST of max-like protein x vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 5


Match Name	E-value	Identity	Description
bigmax	3.977e-23	42.07	gene:FBgn0039509 transcript:FBtr0085173	[more]
Mondo	7.110e-6	39.06	gene:FBgn0032940 transcript:FBtr0081527	[more]
Mondo	7.522e-6	39.06	gene:FBgn0032940 transcript:FBtr0301458	[more]
Mondo	7.522e-6	39.06	gene:FBgn0032940 transcript:FBtr0301457	[more]
Mondo	7.522e-6	39.06	gene:FBgn0032940 transcript:FBtr0301462	[more]

BLAST of max-like protein x vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1


Match Name	E-value	Identity	Description
mlx	6.596e-15	36.20	MAX dimerization protein MLX [Source:ZFIN;Acc:ZDB-...	[more]

BLAST of max-like protein x vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3


Match Name	E-value	Identity	Description
MLX	1.329e-32	37.27	MLX, MAX dimerization protein [Source:NCBI gene;Ac...	[more]
MLX	1.134e-31	39.51	MLX, MAX dimerization protein [Source:NCBI gene;Ac...	[more]
MLX	5.387e-29	42.46	MLX, MAX dimerization protein [Source:NCBI gene;Ac...	[more]

BLAST of max-like protein x vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4


Match Name	E-value	Identity	Description
Mlx	4.198e-25	41.21	MAX-like protein X [Source:MGI Symbol;Acc:MGI:1083...	[more]
Mlx	6.886e-25	41.21	MAX-like protein X [Source:MGI Symbol;Acc:MGI:1083...	[more]
Mlx	1.908e-24	41.21	MAX-like protein X [Source:MGI Symbol;Acc:MGI:1083...	[more]
Mlx	5.655e-7	70.00	MAX-like protein X [Source:MGI Symbol;Acc:MGI:1083...	[more]

BLAST of max-like protein x vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2


Match Name	E-value	Identity	Description
sp\|Q9UH92\|MLX_HUMAN	1.988e-24	40.74	Max-like protein X OS=Homo sapiens OX=9606 GN=MLX ...	[more]
sp\|O08609\|MLX_MOUSE	9.142e-24	40.21	Max-like protein X OS=Mus musculus OX=10090 GN=Mlx...	[more]

BLAST of max-like protein x vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5


Match Name	E-value	Identity	Description
A0A0F7GXZ2	3.038e-158	99.60	Max-like protein x (Fragment) OS=Schmidtea mediter...	[more]
A0A0P4VQ84	6.600e-33	49.72	Putative bhlhzip transcription factor bigmax (Frag...	[more]
T1HED9	3.658e-32	46.15	BHLH domain-containing protein (Fragment) OS=Rhodn...	[more]
A0A224XYZ1	1.914e-31	48.62	Putative bhlhzip transcription factor bigmax (Frag...	[more]
A0A2R5L8J0	2.201e-31	44.27	Putative bhlhzip transcription factor bigmax (Frag...	[more]

BLAST of max-like protein x vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mlx	3.455e-25	42.63	MAX dimerization protein MLX [Source:NCBI gene;Acc...	[more]
mlx	3.556e-25	42.63	MAX dimerization protein MLX [Source:NCBI gene;Acc...	[more]

BLAST of max-like protein x vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 2


Match Name	E-value	Identity	Description
ENSPMAT00000005581.1	4.301e-28	41.62	pep scaffold:Pmarinus_7.0:GL487226:4176:7420:1 gen...	[more]
MLXIPL	2.052e-6	41.67	MLX interacting protein like [Source:HGNC Symbol;A...	[more]

BLAST of max-like protein x vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0


Match Name	E-value	Identity	Description

BLAST of max-like protein x vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 3


Match Name	E-value	Identity	Description
EDO46174	1.655e-27	38.31	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO27547	5.779e-26	42.86	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]
EDO39391	9.812e-6	42.00	Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7...	[more]

BLAST of max-like protein x vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 2


Match Name	E-value	Identity	Description
mlx	4.536e-26	43.79	MAX dimerization protein MLX [Source:NCBI gene;Acc...	[more]
mlx	5.604e-26	43.79	MAX dimerization protein MLX [Source:NCBI gene;Acc...	[more]

BLAST of max-like protein x vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1


Match Name	E-value	Identity	Description
SMESG000025391.1	0.000e+0	99.71	SMESG000025391.1	[more]

Analyses

This transcript is derived from or has results from the following analyses


Analysis Name	Date Performed
Embryonic Stages	2016-07-25
Schmidtea mediterranea smed_20140614 BLASTX Human e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99	2016-08-08
Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL	2020-05-01
Schmidtea mediterranea Sexual smed_20140614 transcriptome	2016-07-25
Schmidtea mediteranean smed_20140614 Interproscan	2020-05-01
Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99	2020-05-08
Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST	2020-05-08

Cross References

External references for this transcript


Database	Accession
SmedGD GBrowse	SMED30010649
GenBank	AKG62113.1

Sequences

The following sequences are available for this feature:

transcript sequence
protein sequence

transcript sequence

>SMED30010649 ID=SMED30010649|Name=max-like protein x|organism=Schmidtea mediterranea sexual|type=transcript|length=1205bp
CGCAATTTTAAAATAGTAATCTCTTAAATTTTTTCATATTATTACATACA
TATTAGTGTAATAATAATAATAATATTAAATACATAATTATTCAAAAGCT
TTAATGTACAATGTTAATAATCCATCAAACATGTATGATCAATCTCAGAT
TGTTAAAATAGCATCATCGGTATCATCACAAAATTCTGATGAAGATATTG
ACACTGCTAATATTATGATAAGGAAGGATCGACGTAGAGAATTACATACT
CAAGCCGAACAACGTCGTAGAGATGCAATTAAGAGTGGGTATGATTCTTT
AATGCCACTTATTCACCCAGCATCAGAAACAAATGGAACTAAACTCAGTA
AAGCAACCATTTTACAAAGAACAATTTCTCAAATAAGGTCATTAAGTGAA
CAAACGAGTATAAAGGAAAAAGAACTGGAGAATTTAAAAAATGAACTGTT
TGCTCTGCGCATAATGAATTCAAACTATGAGGAAATGGCCAAGTGTAACA
AAAATCAAATTAAAACCGAAGAAACAAACGAAGTGCCTGAACAAGTGAAA
TTTGAAATATTCGGCCAATTTCTCCAGCAGTTGTTTCAGTCCTTCGATAA
AAGCACGAAATTTGACAATTTCTCCACAGCCTCAACGACAATCATTCACT
GGGTGGAGGAATACTGCAAACCCCAGAATCTCAATGTTCTGGTCGAATCG
GTTTTAACGGACGTTCTATCGAATTATCAGAGCTCACAAGATAGAAGAAT
GCCTGTTGATATTCTGTCGACAGCCGACATGAGCATGGATCAGGATAACC
TCAGCACTTATCTGGGATCGCTATCTGACTGCCAGCCAAGCTCGAAATCT
TACCGATCGATGAGCAATCCGAGTGTCACCTTCTGTCCATTGGGGAATAA
TGAATCGAAACCCTTCAAAACATCCAACAGAAGGCAGCCAGGAATGCATC
CATCTCAGCAGGTGAATATACAACCCCAGGTATCGCTACCGTCGGTAATT
CAATCACTTGATCCCTCAATCGATGATGAGTTCATGTCAATTTCACCAAA
TGCACTCGAATTGGCATTTCTTGACTCCTTGGATAACAGCACGCTTATCG
ATGATATTTGGCTGCCATCGGAATGATGACTTGATTTAGGTGCTTGTTAT
CAGTTTTCATTGGCTACTTTTTGTTGCAATAAATATTTCGCTGCTTCAAA
AAAAA

protein sequence of SMED30010649-orf-1

>SMED30010649-orf-1 ID=SMED30010649-orf-1|Name=SMED30010649-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=341bp
MYNVNNPSNMYDQSQIVKIASSVSSQNSDEDIDTANIMIRKDRRRELHTQ
AEQRRRDAIKSGYDSLMPLIHPASETNGTKLSKATILQRTISQIRSLSEQ
TSIKEKELENLKNELFALRIMNSNYEEMAKCNKNQIKTEETNEVPEQVKF
EIFGQFLQQLFQSFDKSTKFDNFSTASTTIIHWVEEYCKPQNLNVLVESV
LTDVLSNYQSSQDRRMPVDILSTADMSMDQDNLSTYLGSLSDCQPSSKSY
RSMSNPSVTFCPLGNNESKPFKTSNRRQPGMHPSQQVNIQPQVSLPSVIQ
SLDPSIDDEFMSISPNALELAFLDSLDNSTLIDDIWLPSE*

Annotated Terms

The following terms have been associated with this transcript:

Vocabulary: **Planarian Anatomy**
Term	Definition
PLANA:0000016	pharynx
PLANA:0000026	gut
PLANA:0000099	neuron
PLANA:0000103	central nervous system
PLANA:0000120	progenitor cell
PLANA:0000121	stem cell
PLANA:0002142	parenchyma

Vocabulary: **INTERPRO**
Term	Definition
IPR036638	HLH_DNA-bd_sf
IPR032647	Mlx
IPR011598	bHLH_dom

Vocabulary: **molecular function**
Term	Definition
GO:0046983	protein dimerization activity
GO:0008134	transcription factor binding

Vocabulary: **biological process**
Term	Definition
GO:0006355	regulation of transcription, DNA-templated

InterPro

Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 94..128
None	No IPR available	PANTHER	PTHR15741	BASIC HELIX-LOOP-HELIX ZIP TRANSCRIPTION FACTOR	coord: 20..204
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 49..103 e-value: 5.4E-9 score: 45.9
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 44..97 e-value: 1.8E-11 score: 43.8
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 43..97 score: 14.151
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	CDD	cd00083	HLH	coord: 41..100 e-value: 2.38242E-11 score: 56.4535
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	G3DSA:4.10.280.10		coord: 44..120 e-value: 7.1E-14 score: 53.7
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	SSF47459	HLH, helix-loop-helix DNA-binding domain	coord: 43..126
IPR032647	Max-like protein X	PANTHER	PTHR15741:SF25	MAX-LIKE PROTEIN X	coord: 20..204

Main menu

max-like protein x

Zeng et. al., 2018

▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Overview

Single cell RNA-seq of pluripotent neoblasts and its early progenies

Explore this single cell expression dataset with our NB Cluster Shiny App

Neoblast Population

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

Davies et. al., 2017

PAGE et. al., 2020

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE

PAGE Curations: 7

Owlery

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