Reverse transcriptase domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010644 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Match: A0A0C2N2C4 (Retrovirus-related Pol polyprotein OS=Thelohanellus kitauei OX=669202 GN=RF11_14843 PE=4 SV=1) HSP 1 Score: 56.9954 bits (136), Expect = 6.358e-6 Identity = 34/84 (40.48%), Postives = 44/84 (52.38%), Query Frame = 2 Query: 137 ATQLRSFLGTVNYFHLFIQRFPKFSHHCLVFVQIQRNL*LFGMKFCWTHLKKLKTCLANIQTLHYPKRILELRLTTDASNIAIG 388 AT+L+ FLG YF FI+ F +H CL + +N C KLKT L+ + LH+PK + L L TDAS AIG Sbjct: 656 ATELKRFLGFCGYFQKFIKNFASIAH-CL-YSLTSKNTKFVRSPDCQEAFNKLKTSLSQLPLLHFPKPGIPLHLHTDASGKAIG 737
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000037560.1 (SMESG000037560.1) HSP 1 Score: 177.948 bits (450), Expect = 7.278e-58 Identity = 87/87 (100.00%), Postives = 87/87 (100.00%), Query Frame = -2 Query: 23 MRPAKLHSKQLKISLDLNKDQTVVREFRKSLDKQMKVVYCSQKTSQLSCCLSASSYGHYTAVTCLTKLFIANTYLYCLNAFSIKFTI 283 MRPAKLHSKQLKISLDLNKDQTVVREFRKSLDKQMKVVYCSQKTSQLSCCLSASSYGHYTAVTCLTKLFIANTYLYCLNAFSIKFTI Sbjct: 1 MRPAKLHSKQLKISLDLNKDQTVVREFRKSLDKQMKVVYCSQKTSQLSCCLSASSYGHYTAVTCLTKLFIANTYLYCLNAFSIKFTI 87
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000022029.1 (SMESG000022029.1) HSP 1 Score: 64.3142 bits (155), Expect = 6.163e-13 Identity = 31/40 (77.50%), Postives = 34/40 (85.00%), Query Frame = -1 Query: 399 TQISRELQRTKATWIEPSRVKSIYQRLTVAQRGWQEDKAL 518 Q+S ELQRTKATWIE S VK IYQRLT AQRGW+E+KAL Sbjct: 26 VQLSHELQRTKATWIETSGVKRIYQRLTAAQRGWKEEKAL 65
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000036641.1 (SMESG000036641.1) HSP 1 Score: 62.7734 bits (151), Expect = 1.099e-11 Identity = 42/106 (39.62%), Postives = 62/106 (58.49%), Query Frame = -1 Query: 207 QISRELQRTKATWIEPSRVKSIYQRLTVAQRGWQEDKALKTHRQWLYSKHQLLNVTPKSF*GNVMFVY*LDMF---LAFSNASSKTSFQTTKDFFGFEQRPDSGER 515 Q+SRE+Q+ +ATWIEP+R K+I QRLT AQRGW E+K L +++ ++++ L V + Y L + +A+ +ASS T T K +QRP ER Sbjct: 183 QMSREIQKIRATWIEPARAKAINQRLTAAQRGWAEEKQLVQNQR---TQNRSLEVALLACQEGNAVTYQLVIAPAQIAYRDASSTTKPVTAK---RPQQRPGKTER 282
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000042603.1 (SMESG000042603.1) HSP 1 Score: 58.5362 bits (140), Expect = 7.611e-11 Identity = 26/59 (44.07%), Postives = 41/59 (69.49%), Query Frame = -1 Query: 396 ENNNCIYHWRITRVC*PWTQISRELQRTKATWIEPSRVKSIYQRLTVAQRGWQEDKALK 572 +NN +Y + Q+++E+Q+ K+TW++P+R+KSIYQRLT AQRGW E++ L Sbjct: 16 QNNQSLYGQIL--------QLTKEVQQVKSTWVDPARLKSIYQRLTAAQRGWAEERQLN 66
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Match: SMESG000043233.1 (SMESG000043233.1) HSP 1 Score: 58.151 bits (139), Expect = 9.106e-11 Identity = 25/39 (64.10%), Postives = 33/39 (84.62%), Query Frame = -1 Query: 399 QISRELQRTKATWIEPSRVKSIYQRLTVAQRGWQEDKAL 515 Q++RE+Q+ KATWI+P +VKS+YQRLT AQ GW E+K L Sbjct: 21 QLTREVQQVKATWIDPRKVKSLYQRLTTAQNGWAEEKQL 59 The following BLAST results are available for this feature:
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of Reverse transcriptase domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010644 ID=SMED30010644|Name=Reverse transcriptase domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=632bpback to top Annotated Terms
The following terms have been associated with this transcript:
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