DNA-binding protein inhibitor ID-2

Overview
NameDNA-binding protein inhibitor ID-2
Smed IDSMED30010638
Length (bp)414
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA-binding protein inhibitor ID-2 (SMED30010638) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA-binding protein inhibitor ID-2 (SMED30010638) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA-binding protein inhibitor ID-2 (SMED30010638) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA-binding protein inhibitor ID-2 (SMED30010638) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010638

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 10

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
protonephridiaSMED30010638SMESG000017492.1 dd_Smed_v4_11911_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010638SMESG000017492.1 dd_Smed_v4_11911_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010638SMESG000017492.1 dd_Smed_v4_11911_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010638SMESG000017492.1 dd_Smed_v4_11911_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30010638SMESG000017492.1 dd_Smed_v4_11911_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30010638SMESG000017492.1 dd_Smed_v6_11911_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
central nervous systemSMED30010638SMESG000017492.1 KF487114smed_ncbi_20200123PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
progenitor cellSMED30010638SMESG000017492.1 KF487114smed_ncbi_20200123PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
stem cellSMED30010638SMESG000017492.1 KF487114smed_ncbi_20200123PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
parenchymaSMED30010638SMESG000017492.1 KF487114smed_ncbi_20200123PMID:24173799
Cowles et al., 2013		whole organism asexual adult colorimetric in situ hybridization evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Match: ID1 (inhibitor of DNA binding 1, HLH protein [Source:HGNC Symbol;Acc:HGNC:5360])

HSP 1 Score: 61.2326 bits (147), Expect = 3.258e-12
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   68 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 106
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Match: ID1 (inhibitor of DNA binding 1, HLH protein [Source:HGNC Symbol;Acc:HGNC:5360])

HSP 1 Score: 61.2326 bits (147), Expect = 4.107e-12
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   68 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 106
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Match: ID4 (inhibitor of DNA binding 4, HLH protein [Source:HGNC Symbol;Acc:HGNC:5363])

HSP 1 Score: 60.8474 bits (146), Expect = 6.231e-12
Identity = 24/39 (61.54%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ L++LVPTIP NKKV+++E+LQHVI+YI DL+
Sbjct:   67 DMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQ 105
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Match: ID2 (inhibitor of DNA binding 2 [Source:HGNC Symbol;Acc:HGNC:5361])

HSP 1 Score: 58.5362 bits (140), Expect = 2.616e-11
Identity = 24/38 (63.16%), Postives = 35/38 (92.11%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NKKV+++E+LQHVI+YI DL+
Sbjct:   39 MNDCYSKLKELVPSIPQNKKVSKMEILQHVIDYILDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Match: ID2 (inhibitor of DNA binding 2 [Source:HGNC Symbol;Acc:HGNC:5361])

HSP 1 Score: 58.5362 bits (140), Expect = 2.616e-11
Identity = 24/38 (63.16%), Postives = 35/38 (92.11%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NKKV+++E+LQHVI+YI DL+
Sbjct:   39 MNDCYSKLKELVPSIPQNKKVSKMEILQHVIDYILDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Fly
Match: emc (gene:FBgn0000575 transcript:FBtr0072578)

HSP 1 Score: 46.9802 bits (110), Expect = 6.216e-7
Identity = 21/39 (53.85%), Postives = 32/39 (82.05%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            EMK   ++LK LVP +PKN+K+ ++E++QHVI+YI DL+
Sbjct:   38 EMKMYLSKLKDLVPFMPKNRKLTKLEIIQHVIDYICDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Match: id3 (inhibitor of DNA binding 3 [Source:ZFIN;Acc:ZDB-GENE-020515-1])

HSP 1 Score: 60.077 bits (144), Expect = 3.472e-12
Identity = 24/46 (52.17%), Postives = 37/46 (80.43%), Query Frame = 1 
Query:  112 TRSIKSLEMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +  +  + M  CY++LK+LVP+IP+NK V+Q+E+LQHVI+YI DL+
Sbjct:   34 SEELSDMNMNDCYSKLKELVPSIPQNKSVSQVEILQHVIDYIFDLQ 79
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Match: id4 (inhibitor of DNA binding 4 [Source:ZFIN;Acc:ZDB-GENE-051113-208])

HSP 1 Score: 59.3066 bits (142), Expect = 6.767e-12
Identity = 24/39 (61.54%), Postives = 35/39 (89.74%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPTIP++KKV+++E+LQHVI+YI DL+
Sbjct:   43 DMNDCYSRLKRLVPTIPQDKKVSKVEILQHVIDYILDLQ 81
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Match: id1 (inhibitor of DNA binding 1, HLH protein [Source:ZFIN;Acc:ZDB-GENE-990415-96])

HSP 1 Score: 57.3806 bits (137), Expect = 4.338e-11
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY++LK+LVPT+P NKK +++E+LQHVI+YI DL+
Sbjct:   49 DMNSCYSKLKELVPTLPTNKKASKMEILQHVIDYIWDLQ 87
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Match: id2a (inhibitor of DNA binding 2a [Source:ZFIN;Acc:ZDB-GENE-020910-1])

HSP 1 Score: 57.3806 bits (137), Expect = 5.966e-11
Identity = 23/38 (60.53%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NK V+++E+LQHVI+YI DL+
Sbjct:   43 MNDCYSKLKELVPSIPQNKNVSKMEILQHVIDYILDLQ 80
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Match: id2a (inhibitor of DNA binding 2a [Source:ZFIN;Acc:ZDB-GENE-020910-1])

HSP 1 Score: 57.3806 bits (137), Expect = 5.966e-11
Identity = 23/38 (60.53%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NK V+++E+LQHVI+YI DL+
Sbjct:   43 MNDCYSKLKELVPSIPQNKNVSKMEILQHVIDYILDLQ 80
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Xenopus
Match: id4 (inhibitor of DNA binding 4, HLH protein [Source:NCBI gene;Acc:448114])

HSP 1 Score: 60.077 bits (144), Expect = 6.437e-12
Identity = 25/39 (64.10%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPTIP NKKV+++E+LQHVI+YI DL+
Sbjct:   51 DMNDCYSRLKRLVPTIPPNKKVSKVEILQHVIDYILDLQ 89
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Xenopus
Match: id2 (inhibitor of DNA binding 2 [Source:NCBI gene;Acc:394480])

HSP 1 Score: 58.9214 bits (141), Expect = 1.521e-11
Identity = 24/38 (63.16%), Postives = 35/38 (92.11%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NKKV+++E+LQHVI+YI DL+
Sbjct:   39 MNDCYSKLKELVPSIPQNKKVSKMEILQHVIDYILDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Xenopus
Match: id3 (inhibitor of DNA binding 3, HLH protein [Source:NCBI gene;Acc:549025])

HSP 1 Score: 56.225 bits (134), Expect = 1.448e-10
Identity = 25/53 (47.17%), Postives = 38/53 (71.70%), Query Frame = 1 
Query:   94 HKRLNLTRSIKSL-EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            HK   +   +  L +M  CY++LK+LVP IP+  K++Q+E+LQHVI+YI DL+
Sbjct:   33 HKGPGVDEPMGLLYDMNGCYSKLKELVPGIPQGSKLSQVEILQHVIDYIFDLQ 85
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Match: Id1 (inhibitor of DNA binding 1, HLH protein [Source:MGI Symbol;Acc:MGI:96396])

HSP 1 Score: 60.8474 bits (146), Expect = 3.045e-12
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   61 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 99
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Match: Id1 (inhibitor of DNA binding 1, HLH protein [Source:MGI Symbol;Acc:MGI:96396])

HSP 1 Score: 60.8474 bits (146), Expect = 4.406e-12
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   61 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 99
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Match: Id4 (inhibitor of DNA binding 4 [Source:MGI Symbol;Acc:MGI:99414])

HSP 1 Score: 60.4622 bits (145), Expect = 5.116e-12
Identity = 24/39 (61.54%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ L++LVPTIP NKKV+++E+LQHVI+YI DL+
Sbjct:   67 DMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQ 105
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Match: Id2 (inhibitor of DNA binding 2 [Source:MGI Symbol;Acc:MGI:96397])

HSP 1 Score: 58.151 bits (139), Expect = 2.832e-11
Identity = 24/38 (63.16%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NKKV ++E+LQHVI+YI DL+
Sbjct:   39 MNDCYSKLKELVPSIPQNKKVTKMEILQHVIDYILDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Match: Id2 (inhibitor of DNA binding 2 [Source:MGI Symbol;Acc:MGI:96397])

HSP 1 Score: 58.151 bits (139), Expect = 2.832e-11
Identity = 24/38 (63.16%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NKKV ++E+LQHVI+YI DL+
Sbjct:   39 MNDCYSKLKELVPSIPQNKKVTKMEILQHVIDYILDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Match: sp|P41134|ID1_HUMAN (DNA-binding protein inhibitor ID-1 OS=Homo sapiens OX=9606 GN=ID1 PE=1 SV=3)

HSP 1 Score: 61.2326 bits (147), Expect = 1.973e-11
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   68 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 106
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Match: sp|P41135|ID1_RAT (DNA-binding protein inhibitor ID-1 OS=Rattus norvegicus OX=10116 GN=Id1 PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 2.506e-11
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   61 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 99
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Match: sp|P47928|ID4_HUMAN (DNA-binding protein inhibitor ID-4 OS=Homo sapiens OX=9606 GN=ID4 PE=1 SV=1)

HSP 1 Score: 60.8474 bits (146), Expect = 2.992e-11
Identity = 24/39 (61.54%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ L++LVPTIP NKKV+++E+LQHVI+YI DL+
Sbjct:   67 DMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQ 105
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Match: sp|P20067|ID1_MOUSE (DNA-binding protein inhibitor ID-1 OS=Mus musculus OX=10090 GN=Id1 PE=1 SV=3)

HSP 1 Score: 60.8474 bits (146), Expect = 3.082e-11
Identity = 23/39 (58.97%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPT+P+N+KV+++E+LQHVI+YI+DL+
Sbjct:   61 DMNGCYSRLKELVPTLPQNRKVSKVEILQHVIDYIRDLQ 99
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Match: sp|Q6GL62|ID4_XENTR (DNA-binding protein inhibitor ID-4 OS=Xenopus tropicalis OX=8364 GN=id4 PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 3.410e-11
Identity = 25/39 (64.10%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPTIP NKKV+++E+LQHVI+YI DL+
Sbjct:   51 DMNDCYSRLKRLVPTIPPNKKVSKVEILQHVIDYILDLQ 89
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Match: U5YTC3 (Id4 protein (Fragment) OS=Schmidtea mediterranea OX=79327 GN=id4 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.142e-15
Identity = 50/50 (100.00%), Postives = 50/50 (100.00%), Query Frame = 1 
Query:  163 KLVPTIPKNKKVNQIELLQHVINYIQDLEXXXXXXXXXXXQTEQKLEKSQ 312
            KLVPTIPKNKKVNQIELLQHVINYIQDLELILLLSSKSISQTEQKLEKSQ
Sbjct:    1 KLVPTIPKNKKVNQIELLQHVINYIQDLELILLLSSKSISQTEQKLEKSQ 50
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Match: C1LEX6 (Basic helix-loop-helix dimerisation region bHLH,domain-containing protein OS=Schistosoma japonicum OX=6182 GN=EWB00_009386 PE=2 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.515e-11
Identity = 30/39 (76.92%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            EMK+C  +LKK+VPTI +NKKVNQ+ELLQHVI+YIQDLE
Sbjct:   31 EMKRCLVKLKKMVPTIERNKKVNQLELLQHVIDYIQDLE 69
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Match: A0A4Z2DRI7 (DNA-binding protein inhibitor ID-2-A isoform 2 OS=Schistosoma japonicum OX=6182 GN=EWB00_009386 PE=4 SV=1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.742e-11
Identity = 30/39 (76.92%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            EMK+C  +LKK+VPTI +NKKVNQ+ELLQHVI+YIQDLE
Sbjct:   31 EMKRCLVKLKKMVPTIERNKKVNQLELLQHVIDYIQDLE 69
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Match: A0A430QSA9 (BHLH domain-containing protein OS=Schistosoma bovis OX=6184 GN=DC041_0006509 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.163e-11
Identity = 30/39 (76.92%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            EMK+C  +LKK+VPTI +NKKVNQ+ELLQHVI+YIQDLE
Sbjct:   31 EMKRCLVKLKKMVPTIERNKKVNQLELLQHVIDYIQDLE 69
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Match: A0A183NJG0 (BHLH domain-containing protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS2246 PE=4 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 2.163e-11
Identity = 30/39 (76.92%), Postives = 36/39 (92.31%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            EMK+C  +LKK+VPTI +NKKVNQ+ELLQHVI+YIQDLE
Sbjct:   31 EMKRCLVKLKKMVPTIERNKKVNQLELLQHVIDYIQDLE 69
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Match: id3 (inhibitor of DNA binding 3, HLH protein [Source:NCBI gene;Acc:103027061])

HSP 1 Score: 58.9214 bits (141), Expect = 6.248e-12
Identity = 26/55 (47.27%), Postives = 41/55 (74.55%), Query Frame = 1 
Query:   94 HKRLNLTRSIKSLE---MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
             + L+++RS    E   M  CY+ LK+LVP+IP+++ V+Q+E+LQHVI+YI DL+
Sbjct:   22 EQSLSISRSRSPAELSDMNDCYSRLKELVPSIPQDRSVSQVEILQHVIDYIFDLQ 76
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Match: id4 (inhibitor of DNA binding 4, HLH protein [Source:NCBI gene;Acc:103041967])

HSP 1 Score: 57.7658 bits (138), Expect = 2.307e-11
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ L++LVPTIP +KKV+++E+LQHVI+YI DL+
Sbjct:   46 DMNDCYSRLRRLVPTIPPHKKVSRVEILQHVIDYILDLQ 84
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Match: id4 (inhibitor of DNA binding 4, HLH protein [Source:NCBI gene;Acc:103041967])

HSP 1 Score: 57.7658 bits (138), Expect = 2.307e-11
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ L++LVPTIP +KKV+++E+LQHVI+YI DL+
Sbjct:   46 DMNDCYSRLRRLVPTIPPHKKVSRVEILQHVIDYILDLQ 84
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Match: id1 (DNA-binding protein inhibitor ID-1-like [Source:NCBI gene;Acc:103047891])

HSP 1 Score: 57.7658 bits (138), Expect = 2.815e-11
Identity = 24/39 (61.54%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CYT+LK+LVPT+P NKK +++E+LQHVI+YI DL+
Sbjct:   48 DMNSCYTKLKELVPTLPANKKHSKVEILQHVIDYIWDLQ 86
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Match: ENSAMXT00000051460.1 (DNA-binding protein inhibitor ID-1-like [Source:NCBI gene;Acc:103045393])

HSP 1 Score: 56.9954 bits (136), Expect = 5.006e-11
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY++LK+LVPT+P NKK +++E+LQHVI+YI DL+
Sbjct:   41 DMNSCYSKLKELVPTLPANKKHSKVEILQHVIDYIWDLQ 79
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Sea Lamprey
Match: id4 (inhibitor of DNA binding 4 [Source:ZFIN;Acc:ZDB-GENE-051113-208])

HSP 1 Score: 47.3654 bits (111), Expect = 2.726e-8
Identity = 20/40 (50.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPK-NKKVNQIELLQHVINYIQDLE 249
            +MK CY+ L +LVPT+    +K +++E+LQHVI+YI DL+
Sbjct:   32 DMKGCYSRLSQLVPTLATTGRKASRMEILQHVIDYILDLQ 71
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Nematostella
Match: EDO44627 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RVE5])

HSP 1 Score: 47.7506 bits (112), Expect = 7.013e-8
Identity = 17/38 (44.74%), Postives = 30/38 (78.95%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M +CY  LK +VP +P  ++++++E+LQ+VI+YI DL+
Sbjct:   43 MSECYERLKNMVPNVPVGRRISKVEILQYVIDYILDLQ 80
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Medaka
Match: id3 (inhibitor of DNA binding 3, HLH protein [Source:NCBI gene;Acc:101164112])

HSP 1 Score: 60.077 bits (144), Expect = 2.662e-12
Identity = 30/67 (44.78%), Postives = 45/67 (67.16%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLEXXXXXXXXXXXQTEQKLEKSQ*KSNFNK 333
            +M  CY+ LK+LVP+IP+NK V+Q+E+LQHVI+YI DL++ L     S  +    ++ S    NF+K
Sbjct:   45 DMNDCYSRLKELVPSIPQNKSVSQVEILQHVIDYIFDLQIALEAVDPSAPEMVLSIKTSDITRNFSK 111
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Medaka
Match: id4 (DNA-binding protein inhibitor ID-4 [Source:NCBI gene;Acc:101162314])

HSP 1 Score: 58.9214 bits (141), Expect = 1.269e-11
Identity = 24/39 (61.54%), Postives = 35/39 (89.74%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY+ LK+LVPTIP++KKV+++E+LQHVI+YI DL+
Sbjct:   47 DMNDCYSRLKRLVPTIPQDKKVSKVEILQHVIDYILDLQ 85
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Medaka
Match: id1 (DNA-binding protein inhibitor ID-1 [Source:NCBI gene;Acc:101161879])

HSP 1 Score: 58.9214 bits (141), Expect = 1.544e-11
Identity = 23/39 (58.97%), Postives = 34/39 (87.18%), Query Frame = 1 
Query:  133 EMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            +M  CY++LK+LVPT+P NKK +++E+LQHVI+YI DL+
Sbjct:   75 DMNSCYSKLKELVPTLPTNKKASKVEILQHVIDYIWDLQ 113
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Medaka
Match: id2a (inhibitor of DNA binding 2 [Source:NCBI gene;Acc:101169826])

HSP 1 Score: 56.9954 bits (136), Expect = 6.544e-11
Identity = 23/38 (60.53%), Postives = 34/38 (89.47%), Query Frame = 1 
Query:  136 MKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLE 249
            M  CY++LK+LVP+IP+NK V+++E+LQHVI+YI DL+
Sbjct:   41 MNDCYSKLKELVPSIPQNKNVSKMEILQHVIDYILDLQ 78
[Close]
BLAST of DNA-binding protein inhibitor ID-2 vs. Planmine SMEST
Match: SMESG000017492.1 (SMESG000017492.1)

HSP 1 Score: 121.709 bits (304), Expect = 3.693e-37
Identity = 74/74 (100.00%), Postives = 74/74 (100.00%), Query Frame = 1 
Query:   91 MHKRLNLTRSIKSLEMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLEXXXXXXXXXXXQTEQKLEKSQ 312
            MHKRLNLTRSIKSLEMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLELILLLSSKSISQTEQKLEKSQ
Sbjct:    1 MHKRLNLTRSIKSLEMKKCYTELKKLVPTIPKNKKVNQIELLQHVINYIQDLELILLLSSKSISQTEQKLEKSQ 74
The following BLAST results are available for this feature:
BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
ID13.258e-1258.97inhibitor of DNA binding 1, HLH protein [Source:HG... [more]
ID14.107e-1258.97inhibitor of DNA binding 1, HLH protein [Source:HG... [more]
ID46.231e-1261.54inhibitor of DNA binding 4, HLH protein [Source:HG... [more]
ID22.616e-1163.16inhibitor of DNA binding 2 [Source:HGNC Symbol;Acc... [more]
ID22.616e-1163.16inhibitor of DNA binding 2 [Source:HGNC Symbol;Acc... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
emc6.216e-753.85gene:FBgn0000575 transcript:FBtr0072578[more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
id33.472e-1252.17inhibitor of DNA binding 3 [Source:ZFIN;Acc:ZDB-GE... [more]
id46.767e-1261.54inhibitor of DNA binding 4 [Source:ZFIN;Acc:ZDB-GE... [more]
id14.338e-1158.97inhibitor of DNA binding 1, HLH protein [Source:ZF... [more]
id2a5.966e-1160.53inhibitor of DNA binding 2a [Source:ZFIN;Acc:ZDB-G... [more]
id2a5.966e-1160.53inhibitor of DNA binding 2a [Source:ZFIN;Acc:ZDB-G... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 3
Match NameE-valueIdentityDescription
id46.437e-1264.10inhibitor of DNA binding 4, HLH protein [Source:NC... [more]
id21.521e-1163.16inhibitor of DNA binding 2 [Source:NCBI gene;Acc:3... [more]
id31.448e-1047.17inhibitor of DNA binding 3, HLH protein [Source:NC... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 5
Match NameE-valueIdentityDescription
Id13.045e-1258.97inhibitor of DNA binding 1, HLH protein [Source:MG... [more]
Id14.406e-1258.97inhibitor of DNA binding 1, HLH protein [Source:MG... [more]
Id45.116e-1261.54inhibitor of DNA binding 4 [Source:MGI Symbol;Acc:... [more]
Id22.832e-1163.16inhibitor of DNA binding 2 [Source:MGI Symbol;Acc:... [more]
Id22.832e-1163.16inhibitor of DNA binding 2 [Source:MGI Symbol;Acc:... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P41134|ID1_HUMAN1.973e-1158.97DNA-binding protein inhibitor ID-1 OS=Homo sapiens... [more]
sp|P41135|ID1_RAT2.506e-1158.97DNA-binding protein inhibitor ID-1 OS=Rattus norve... [more]
sp|P47928|ID4_HUMAN2.992e-1161.54DNA-binding protein inhibitor ID-4 OS=Homo sapiens... [more]
sp|P20067|ID1_MOUSE3.082e-1158.97DNA-binding protein inhibitor ID-1 OS=Mus musculus... [more]
sp|Q6GL62|ID4_XENTR3.410e-1164.10DNA-binding protein inhibitor ID-4 OS=Xenopus trop... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
U5YTC32.142e-15100.00Id4 protein (Fragment) OS=Schmidtea mediterranea O... [more]
C1LEX61.515e-1176.92Basic helix-loop-helix dimerisation region bHLH,do... [more]
A0A4Z2DRI71.742e-1176.92DNA-binding protein inhibitor ID-2-A isoform 2 OS=... [more]
A0A430QSA92.163e-1176.92BHLH domain-containing protein OS=Schistosoma bovi... [more]
A0A183NJG02.163e-1176.92BHLH domain-containing protein OS=Schistosoma matt... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 5
Match NameE-valueIdentityDescription
id36.248e-1247.27inhibitor of DNA binding 3, HLH protein [Source:NC... [more]
id42.307e-1158.97inhibitor of DNA binding 4, HLH protein [Source:NC... [more]
id42.307e-1158.97inhibitor of DNA binding 4, HLH protein [Source:NC... [more]
id12.815e-1161.54DNA-binding protein inhibitor ID-1-like [Source:NC... [more]
ENSAMXT00000051460.15.006e-1158.97DNA-binding protein inhibitor ID-1-like [Source:NC... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
id42.726e-850.00inhibitor of DNA binding 4 [Source:ZFIN;Acc:ZDB-GE... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO446277.013e-844.74Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 4
Match NameE-valueIdentityDescription
id32.662e-1244.78inhibitor of DNA binding 3, HLH protein [Source:NC... [more]
id41.269e-1161.54DNA-binding protein inhibitor ID-4 [Source:NCBI ge... [more]
id11.544e-1158.97DNA-binding protein inhibitor ID-1 [Source:NCBI ge... [more]
id2a6.544e-1160.53inhibitor of DNA binding 2 [Source:NCBI gene;Acc:1... [more]
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BLAST of DNA-binding protein inhibitor ID-2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000017492.13.693e-37100.00SMESG000017492.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010638 ID=SMED30010638|Name=DNA-binding protein inhibitor ID-2|organism=Schmidtea mediterranea sexual|type=transcript|length=414bp
AGATCTCACTTTTGTATATTTAAGAGTCGTAGATATAATTTCGGTAATTC
AATGACAATACATTTTTATAAGAAAACTCAGTATTACAACATGCATAAAC
GTTTGAATTTAACGCGTTCAATCAAGTCTTTAGAAATGAAAAAATGTTAT
ACTGAATTAAAAAAACTCGTTCCAACGATTCCGAAAAACAAGAAAGTTAA
TCAAATAGAATTGTTGCAGCATGTCATTAATTATATACAAGATTTGGAAT
TAATTCTGTTGCTATCATCAAAATCGATTTCTCAGACTGAACAGAAATTA
GAGAAGTCACAATGAAAATCTAATTTTAATAAATCCAGAAATTGAATTTT
CCTCATTGTTCCTGTATCTATAAATCTACGTCTTTTTTTTGTAAATAAAG
TTTCGATTTAAAAA
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protein sequence of SMED30010638-orf-1

>SMED30010638-orf-1 ID=SMED30010638-orf-1|Name=SMED30010638-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=105bp
RSHFCIFKSRRYNFGNSMTIHFYKKTQYYNMHKRLNLTRSIKSLEMKKCY
TELKKLVPTIPKNKKVNQIELLQHVINYIQDLELILLLSSKSISQTEQKL
EKSQ*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: biological process
TermDefinition
GO:0000122negative regulation of transcription from RNA polymerase II promoter
GO:0001889liver development
GO:0007275multicellular organism development
GO:0008593regulation of Notch signaling pathway
GO:0010468regulation of gene expression
GO:0010628positive regulation of gene expression
GO:0010629negative regulation of gene expression
GO:0031573intra-S DNA damage checkpoint
GO:0042752regulation of circadian rhythm
GO:0045664regulation of neuron differentiation
GO:0045777positive regulation of blood pressure
GO:0045893positive regulation of transcription, DNA-templated
GO:0046532regulation of photoreceptor cell differentiation
GO:0048511rhythmic process
GO:0048557embryonic digestive tract morphogenesis
GO:0048661positive regulation of smooth muscle cell proliferation
GO:0048663neuron fate commitment
GO:0061031endodermal digestive tract morphogenesis
GO:0071158positive regulation of cell cycle arrest
GO:0090398cellular senescence
GO:1902866regulation of retina development in camera-type eye
GO:2000177regulation of neural precursor cell proliferation
GO:2000495regulation of cell proliferation involved in compound eye morphogenesis
GO:0045892negative regulation of transcription, DNA-templated
Vocabulary: cellular component
TermDefinition
GO:0032991macromolecular complex
GO:0005634nucleus
GO:0005737cytoplasm
Vocabulary: molecular function
TermDefinition
GO:0043425bHLH transcription factor binding
GO:0046983protein dimerization activity
GO:0003677DNA binding
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000020protonephridia
PLANA:0000099neuron
PLANA:0000103central nervous system
PLANA:0000120progenitor cell
PLANA:0000121stem cell
PLANA:0000418head
PLANA:0002142parenchyma
Vocabulary: INTERPRO
TermDefinition
IPR036638HLH_DNA-bd_sf
IPR026052DNA-bd_prot-inh
IPR011598bHLH_dom
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 92..103
IPR036638Helix-loop-helix DNA-binding domain superfamilyGENE3DG3DSA:4.10.280.10coord: 18..84
e-value: 2.4E-18
score: 67.7
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILYSSF47459HLH, helix-loop-helix DNA-binding domaincoord: 44..96
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPFAMPF00010HLHcoord: 45..82
e-value: 3.5E-9
score: 36.5
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainPROSITEPS50888BHLHcoord: 29..81
score: 10.81
IPR011598Myc-type, basic helix-loop-helix (bHLH) domainCDDcd00083HLHcoord: 44..82
e-value: 3.80167E-8
score: 44.1271
IPR026052DNA-binding protein inhibitorPANTHERPTHR11723DNA-BINDING PROTEIN INHIBITORcoord: 43..95