E3 ubiquitin-protein ligase RGLG4
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010632 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 17
Homology
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 105.916 bits (263), Expect = 1.631e-24 Identity = 59/209 (28.23%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 188 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQ-----------HNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 375 HSP 2 Score: 90.5077 bits (223), Expect = 3.009e-19 Identity = 44/153 (28.76%), Postives = 81/153 (52.94%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 218 GFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 82.4185 bits (202), Expect = 1.519e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 403 HSP 4 Score: 76.2554 bits (186), Expect = 1.643e-14 Identity = 58/254 (22.83%), Postives = 107/254 (42.13%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFINRNDEIAFDKI---------WKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ V+ F + + I + DF N + LHV N +VK +LD+ Sbjct: 27 SYLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNAN---MVKLLLDR 273 HSP 5 Score: 60.077 bits (144), Expect = 3.332e-9 Identity = 28/97 (28.87%), Postives = 51/97 (52.58%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC-----NIEC 474 ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ N+ C Sbjct: 353 ALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVSC 449
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Match: ANK2 (ankyrin 2 [Source:HGNC Symbol;Acc:HGNC:493]) HSP 1 Score: 102.834 bits (255), Expect = 5.554e-23 Identity = 58/206 (28.16%), Postives = 104/206 (50.49%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ N + ++ G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ ++KLLL+R I+ + G+ P+HCA+ GH Q+++LLL+R + + S L +A Q ++CV+ L+ + D++ D + + LHV A C R+ K +LDK Sbjct: 158 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPV------DDVTLDYL------------TALHV---AAHCGHYRVTKLLLDK 342 HSP 2 Score: 99.3673 bits (246), Expect = 7.231e-22 Identity = 47/140 (33.57%), Postives = 80/140 (57.14%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G V VA ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH ++++LLLER + G+ P+H A+ G H++ +K LLQ K +D Sbjct: 175 LQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 314 HSP 3 Score: 92.4337 bits (228), Expect = 1.412e-19 Identity = 55/203 (27.09%), Postives = 91/203 (44.83%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H+A G + ++++D G I+ +T G+ P+H A+ G +++ LLLER + T +G+ P+H A+ G GH + KLLL++ N G+ PIH A+ GHL I+ LLLQ D N E+ L +A ++ Q++ V+ L+ L D R ++ Sbjct: 221 GITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALSGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPL 423 HSP 4 Score: 88.1965 bits (217), Expect = 3.514e-18 Identity = 45/159 (28.30%), Postives = 77/159 (48.43%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 + + +G ++H+A G VEV R ++ G+ ++ P+H AS G EI+ LLL+ + T +G P+H ++ G ++ +LLE +G P+H A+ G L + KLLLQR+ D + L +A + QM L++ Sbjct: 381 VTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLN 539 HSP 5 Score: 83.5741 bits (205), Expect = 1.151e-16 Identity = 47/183 (25.68%), Postives = 79/183 (43.17%), Query Frame = 1 Query: 97 QCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 Q LQH+ +A G+ +HI+ G V+VA ++++ G+ T G P+H A+ G L++ LLL+R + +G P+H A+ +I LL +G+ P+H AS GH ++ LLL + I + L LA Q +++ +L Sbjct: 436 QLLLQHMAHPDA---------------ATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 603 HSP 6 Score: 79.7221 bits (195), Expect = 2.405e-15 Identity = 62/248 (25.00%), Postives = 104/248 (41.94%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFI---------NRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 + GN D V +YLK + G ++H+A G V + + ++ GS+++ T G +H AS G E++ +L++ +I ++ +G P++ A+ H +++K LLE N G P+ A GH Q + +LL+ D K + L +A + LL+ VQ F N N + DF N + LHV N +VK +LD+ Sbjct: 3 RAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTN---MVKLLLDR 243 HSP 7 Score: 78.9518 bits (193), Expect = 3.979e-15 Identity = 41/149 (27.52%), Postives = 70/149 (46.98%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G E+ ++++ ++ + TT G P+H ++ G +++ ++LLE T G P+H A+ G ++ KLLL+R + G P+H A+ +QI LL + + L LA Q D V LL+ Sbjct: 423 LHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLL 571 HSP 8 Score: 75.485 bits (184), Expect = 5.318e-14 Identity = 39/177 (22.03%), Postives = 83/177 (46.89%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +EG D L++ +++ +G +H+A G ++VA++++ + + G P+H A+ + ++I + LL + T G+ P+H AS GH++++ LLL++ NI G+ +H A+ + + +L + D + + L +A + V L+ Sbjct: 461 REGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGYTPLHIAAKKNQMQIASTLLNYGAETNIVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLL 637 HSP 9 Score: 75.0998 bits (183), Expect = 7.018e-14 Identity = 37/145 (25.52%), Postives = 70/145 (48.28%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELA 609 IV QG +H+A G ++ +++D G+NI T G+ +H A+ + + +L + D + T G P+ A G+ +++ LL++ N+ + G P+H A+ GH I+ +LLQ + + + L +A Sbjct: 546 IVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADQDAHTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIA 690 HSP 10 Score: 73.559 bits (179), Expect = 2.070e-13 Identity = 39/150 (26.00%), Postives = 70/150 (46.67%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V ++++D +N G+ PIH A+ G L I+ LLL+ + G +H A+ G E+++ LL ++ P+H AS G +I++LLLQ D + L ++ + Q+D +L+ Sbjct: 323 ALHVAAHCGHYRVTKLLLDKRANPNARALSGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLL 472 HSP 11 Score: 68.5514 bits (166), Expect = 9.771e-12 Identity = 39/153 (25.49%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G VE + ++ + ++ T + +H A+ G + LLL++ + SG+ PIH A+ GH I+ LLL+ + + + RG +H A+ G +++++ LL+ +D + ++ L +A + + + VQLL+ Sbjct: 287 GLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALSGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 439 HSP 12 Score: 67.0106 bits (162), Expect = 2.457e-11 Identity = 36/165 (21.82%), Postives = 74/165 (44.85%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G IH+A G + + +++ G++ + G +H A+ G +E++ LL ++ P+H AS G +EI++LLL+ + + G P+H ++ G + + +LL+ + L +A + +D +LL+ + D +N Sbjct: 353 GLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKN 517
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 102.834 bits (255), Expect = 7.149e-23 Identity = 59/209 (28.23%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 199 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 386 HSP 2 Score: 88.9669 bits (219), Expect = 2.504e-18 Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 216 LQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 381 HSP 3 Score: 81.6481 bits (200), Expect = 5.208e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 266 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 414 HSP 4 Score: 79.337 bits (194), Expect = 3.538e-15 Identity = 43/163 (26.38%), Postives = 85/163 (52.15%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 351 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 513 HSP 5 Score: 76.6406 bits (187), Expect = 2.411e-14 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 625 GYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 777 HSP 6 Score: 76.6406 bits (187), Expect = 2.432e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 592 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 744 HSP 7 Score: 76.2554 bits (186), Expect = 3.181e-14 Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H ++ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 459 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 612 HSP 8 Score: 76.2554 bits (186), Expect = 3.542e-14 Identity = 45/175 (25.71%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G AD V + L+ G+ +H++ G +VA ++D G+++ T G P+H A+ G LE+ LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+ Sbjct: 504 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 678 HSP 9 Score: 72.4034 bits (176), Expect = 5.633e-13 Identity = 44/182 (24.18%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 39 YLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 216 HSP 10 Score: 70.8626 bits (172), Expect = 1.922e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 271 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 480 HSP 11 Score: 70.4774 bits (171), Expect = 2.196e-12 Identity = 32/125 (25.60%), Postives = 64/125 (51.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G P+H A+ GH I+ +LLQ Sbjct: 654 VTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 778 HSP 12 Score: 65.855 bits (159), Expect = 7.650e-11 Identity = 31/116 (26.72%), Postives = 63/116 (54.31%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G P+H A+ GH+ I+ +LL+ N + Sbjct: 667 QEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 782 HSP 13 Score: 65.4698 bits (158), Expect = 1.060e-10 Identity = 38/156 (24.36%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G++ TT G P+H ++ G ++ LL+ + T G P+H A+ G E+ LLL+++ + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 492 DDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLL 645 HSP 14 Score: 64.3142 bits (155), Expect = 2.407e-10 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L L+ + D L+ Sbjct: 394 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLL 546 HSP 15 Score: 57.3806 bits (137), Expect = 3.608e-8 Identity = 32/157 (20.38%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H ++ GH + LL + + L +A + +++ LL+ Sbjct: 423 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 579
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 102.834 bits (255), Expect = 7.153e-23 Identity = 59/209 (28.23%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 188 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 375 HSP 2 Score: 88.9669 bits (219), Expect = 2.505e-18 Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 81.6481 bits (200), Expect = 5.212e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 403 HSP 4 Score: 79.337 bits (194), Expect = 3.540e-15 Identity = 43/163 (26.38%), Postives = 85/163 (52.15%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 5 Score: 76.6406 bits (187), Expect = 2.412e-14 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 766 HSP 6 Score: 76.6406 bits (187), Expect = 2.434e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 733 HSP 7 Score: 76.2554 bits (186), Expect = 3.183e-14 Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H ++ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 8 Score: 76.2554 bits (186), Expect = 3.544e-14 Identity = 45/175 (25.71%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G AD V + L+ G+ +H++ G +VA ++D G+++ T G P+H A+ G LE+ LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+ Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 667 HSP 9 Score: 72.4034 bits (176), Expect = 5.636e-13 Identity = 44/182 (24.18%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 10 Score: 70.8626 bits (172), Expect = 1.923e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 469 HSP 11 Score: 70.4774 bits (171), Expect = 2.197e-12 Identity = 32/125 (25.60%), Postives = 64/125 (51.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G P+H A+ GH I+ +LLQ Sbjct: 643 VTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 767 HSP 12 Score: 65.855 bits (159), Expect = 7.654e-11 Identity = 31/116 (26.72%), Postives = 63/116 (54.31%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G P+H A+ GH+ I+ +LL+ N + Sbjct: 656 QEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 771 HSP 13 Score: 65.4698 bits (158), Expect = 1.061e-10 Identity = 38/156 (24.36%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G++ TT G P+H ++ G ++ LL+ + T G P+H A+ G E+ LLL+++ + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 481 DDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLL 634 HSP 14 Score: 64.3142 bits (155), Expect = 2.409e-10 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L L+ + D L+ Sbjct: 383 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLL 535 HSP 15 Score: 57.3806 bits (137), Expect = 3.610e-8 Identity = 32/157 (20.38%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H ++ GH + LL + + L +A + +++ LL+ Sbjct: 412 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 568
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Match: ANK3 (ankyrin 3 [Source:HGNC Symbol;Acc:HGNC:494]) HSP 1 Score: 102.834 bits (255), Expect = 7.895e-23 Identity = 59/209 (28.23%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 205 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 392 HSP 2 Score: 88.9669 bits (219), Expect = 2.750e-18 Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 222 LQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 387 HSP 3 Score: 81.6481 bits (200), Expect = 5.698e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 272 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 420 HSP 4 Score: 79.337 bits (194), Expect = 3.865e-15 Identity = 43/163 (26.38%), Postives = 85/163 (52.15%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 357 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 519 HSP 5 Score: 76.6406 bits (187), Expect = 2.628e-14 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 631 GYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 783 HSP 6 Score: 76.6406 bits (187), Expect = 2.652e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 598 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 750 HSP 7 Score: 76.2554 bits (186), Expect = 3.467e-14 Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H ++ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 465 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 618 HSP 8 Score: 76.2554 bits (186), Expect = 3.860e-14 Identity = 45/175 (25.71%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G AD V + L+ G+ +H++ G +VA ++D G+++ T G P+H A+ G LE+ LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+ Sbjct: 510 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 684 HSP 9 Score: 72.4034 bits (176), Expect = 6.121e-13 Identity = 44/182 (24.18%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 45 YLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 222 HSP 10 Score: 70.8626 bits (172), Expect = 2.086e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 277 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 486 HSP 11 Score: 70.4774 bits (171), Expect = 2.382e-12 Identity = 32/125 (25.60%), Postives = 64/125 (51.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G P+H A+ GH I+ +LLQ Sbjct: 660 VTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 784 HSP 12 Score: 65.855 bits (159), Expect = 8.262e-11 Identity = 31/116 (26.72%), Postives = 63/116 (54.31%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G P+H A+ GH+ I+ +LL+ N + Sbjct: 673 QEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 788 HSP 13 Score: 65.4698 bits (158), Expect = 1.145e-10 Identity = 38/156 (24.36%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G++ TT G P+H ++ G ++ LL+ + T G P+H A+ G E+ LLL+++ + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 498 DDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLL 651 HSP 14 Score: 64.3142 bits (155), Expect = 2.596e-10 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L L+ + D L+ Sbjct: 400 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLL 552 HSP 15 Score: 57.3806 bits (137), Expect = 3.860e-8 Identity = 32/157 (20.38%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H ++ GH + LL + + L +A + +++ LL+ Sbjct: 429 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 585
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 80.4925 bits (197), Expect = 5.767e-16 Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L++ + + +HIA G EVA I++D ++ T G P+H AS G LE++ LLLER ++ E + + P+H A+ + ++ LLLE + + G P+H A+ ++I LLQ K + K+ + L L+ Q + LLI Sbjct: 502 GNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLI 676 HSP 2 Score: 78.5666 bits (192), Expect = 2.160e-15 Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G V VA++++D ++ G P+H A + ++++ LLL+ IE T SG+ P+H A+ G I+ LL++ N + E RG P+H A+ ++++L++ ++D + ++ L +A + D V LL+ Sbjct: 363 LHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511 HSP 3 Score: 77.411 bits (189), Expect = 5.845e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A +V R++I G+ ++ + P+H AS G +I+ LLL+ + T P+H A+ G E+ +LL+ N + +G P+H AS G+L++++LLL+R +D + + L +A LL+ Sbjct: 457 RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLL 610 HSP 4 Score: 76.6406 bits (187), Expect = 9.565e-15 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G V+ AR ++ + ++ T + P+H A+ G + + LLL+R+ D +G P+H A ++++LLL+ IE G+ P+H A+ G + I+ LLQ+ D + E+ L LA ++ Q D V++LI Sbjct: 326 GLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478 HSP 5 Score: 75.485 bits (184), Expect = 2.010e-14 Identity = 41/162 (25.31%), Postives = 82/162 (50.62%), Query Frame = 1 Query: 172 TIVDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T VD +G + H+A +VA ++++ G++ + G P+H A+ + +EI + LL+ D ++ +G P+H ++ GH EI LL+E ++ + N G+ +H + H+ + ++L I+ K N + L +A Q++ V+ L+ Sbjct: 581 TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLV 742 HSP 6 Score: 75.0998 bits (183), Expect = 2.651e-14 Identity = 51/209 (24.40%), Postives = 93/209 (44.50%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA A +++ N + + G P+H A+ G + LLLE+ ++ + + P+H A+ G + + LLL R I+ + P+HCA+ GH Q++ LL+ + I K + L +A Q +D + L+ + D + + D++ + LHV A C R+ K +LD++ D Sbjct: 197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------------TVDYL-----TPLHV---AAHCGHVRVAKLLLDRSAD 384 HSP 7 Score: 73.9442 bits (180), Expect = 7.809e-14 Identity = 39/153 (25.49%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ + IE T G+ P+H A+ G + I+ LL++ + + ET G P+H A+ ++++++L+ ++ + P+H AS G+ I+ LLLQ + + S L +A + Q + +L+ Sbjct: 392 GFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILL 544 HSP 8 Score: 73.559 bits (179), Expect = 8.387e-14 Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G V +++++ G+N+ + + P+H A+ G + LLL R I+ T + P+H A+ GH +++ LL+ + I + G+ P+H A+ G H+ + LL + +D Sbjct: 222 VTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVD 353 HSP 9 Score: 73.559 bits (179), Expect = 9.251e-14 Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A ++ ++ ++ G P+H ++ G EI LL+E D+ + N+G+ +H + H + ++L I + N G P+H A G L ++K L++ + K S + L A Q +CV+ L+ Sbjct: 623 GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLL 775 HSP 10 Score: 73.559 bits (179), Expect = 9.417e-14 Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 +HIA G ++ +++ G+N T P+H A+ G E+ +LL+ N D T G P+H AS G+ E+++LLLER ++ E + P+H A+ + ++ LLL+ + L +A + QM+ L+ + Sbjct: 495 LHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK 646 HSP 11 Score: 72.4034 bits (176), Expect = 2.421e-13 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 KEG + L + T++ +G +H+A G +EV R++++ G+ ++ E + P+H A+ ++ LLLE + +G P+H A+ EI LL+ + + G P+H ++ GH +I LL++ + K N + + L Q + Q+L Sbjct: 533 KEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQIL 708 HSP 12 Score: 69.3218 bits (168), Expect = 2.061e-12 Identity = 50/213 (23.47%), Postives = 98/213 (46.01%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + G+ + V + L++ + G S+H+A G EV R +I + ++ T G +H AS G I+T+L+E ++ ++ +G P++ A+ H E++K LL+ N G P+ A GH +++ +LL+ D K + L +A + LL+ + D +++ F + ++ + EN+ Sbjct: 37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKS---GFTPLHIAAHYGHENV 242 HSP 13 Score: 61.2326 bits (147), Expect = 7.743e-10 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 + G ++H+ V VA+I+ + G+ I +T G P+H A G L ++ L+E D+ +T + P+H A+ GH+ ++ LLE + + G P+ A G++ +++ L Sbjct: 687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 14 Score: 58.5362 bits (140), Expect = 5.763e-9 Identity = 32/152 (21.05%), Postives = 72/152 (47.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 G +H++ G E++ ++I+ GS++ + G+ +H + + + +L +I +TN+G P+H A G ++K L+E ++ + P+H A+ GH ++ LL+ + + ++ L +A + + V+ L Sbjct: 656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 80.4925 bits (197), Expect = 5.767e-16 Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L++ + + +HIA G EVA I++D ++ T G P+H AS G LE++ LLLER ++ E + + P+H A+ + ++ LLLE + + G P+H A+ ++I LLQ K + K+ + L L+ Q + LLI Sbjct: 502 GNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLI 676 HSP 2 Score: 78.5666 bits (192), Expect = 2.160e-15 Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G V VA++++D ++ G P+H A + ++++ LLL+ IE T SG+ P+H A+ G I+ LL++ N + E RG P+H A+ ++++L++ ++D + ++ L +A + D V LL+ Sbjct: 363 LHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511 HSP 3 Score: 77.411 bits (189), Expect = 5.845e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A +V R++I G+ ++ + P+H AS G +I+ LLL+ + T P+H A+ G E+ +LL+ N + +G P+H AS G+L++++LLL+R +D + + L +A LL+ Sbjct: 457 RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLL 610 HSP 4 Score: 76.6406 bits (187), Expect = 9.565e-15 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G V+ AR ++ + ++ T + P+H A+ G + + LLL+R+ D +G P+H A ++++LLL+ IE G+ P+H A+ G + I+ LLQ+ D + E+ L LA ++ Q D V++LI Sbjct: 326 GLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478 HSP 5 Score: 75.485 bits (184), Expect = 2.010e-14 Identity = 41/162 (25.31%), Postives = 82/162 (50.62%), Query Frame = 1 Query: 172 TIVDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T VD +G + H+A +VA ++++ G++ + G P+H A+ + +EI + LL+ D ++ +G P+H ++ GH EI LL+E ++ + N G+ +H + H+ + ++L I+ K N + L +A Q++ V+ L+ Sbjct: 581 TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLV 742 HSP 6 Score: 75.0998 bits (183), Expect = 2.651e-14 Identity = 51/209 (24.40%), Postives = 93/209 (44.50%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA A +++ N + + G P+H A+ G + LLLE+ ++ + + P+H A+ G + + LLL R I+ + P+HCA+ GH Q++ LL+ + I K + L +A Q +D + L+ + D + + D++ + LHV A C R+ K +LD++ D Sbjct: 197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------------TVDYL-----TPLHV---AAHCGHVRVAKLLLDRSAD 384 HSP 7 Score: 73.9442 bits (180), Expect = 7.809e-14 Identity = 39/153 (25.49%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ + IE T G+ P+H A+ G + I+ LL++ + + ET G P+H A+ ++++++L+ ++ + P+H AS G+ I+ LLLQ + + S L +A + Q + +L+ Sbjct: 392 GFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILL 544 HSP 8 Score: 73.559 bits (179), Expect = 8.387e-14 Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G V +++++ G+N+ + + P+H A+ G + LLL R I+ T + P+H A+ GH +++ LL+ + I + G+ P+H A+ G H+ + LL + +D Sbjct: 222 VTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVD 353 HSP 9 Score: 73.559 bits (179), Expect = 9.251e-14 Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A ++ ++ ++ G P+H ++ G EI LL+E D+ + N+G+ +H + H + ++L I + N G P+H A G L ++K L++ + K S + L A Q +CV+ L+ Sbjct: 623 GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLL 775 HSP 10 Score: 73.559 bits (179), Expect = 9.417e-14 Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 +HIA G ++ +++ G+N T P+H A+ G E+ +LL+ N D T G P+H AS G+ E+++LLLER ++ E + P+H A+ + ++ LLL+ + L +A + QM+ L+ + Sbjct: 495 LHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK 646 HSP 11 Score: 72.4034 bits (176), Expect = 2.421e-13 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 KEG + L + T++ +G +H+A G +EV R++++ G+ ++ E + P+H A+ ++ LLLE + +G P+H A+ EI LL+ + + G P+H ++ GH +I LL++ + K N + + L Q + Q+L Sbjct: 533 KEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQIL 708 HSP 12 Score: 69.3218 bits (168), Expect = 2.061e-12 Identity = 50/213 (23.47%), Postives = 98/213 (46.01%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + G+ + V + L++ + G S+H+A G EV R +I + ++ T G +H AS G I+T+L+E ++ ++ +G P++ A+ H E++K LL+ N G P+ A GH +++ +LL+ D K + L +A + LL+ + D +++ F + ++ + EN+ Sbjct: 37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKS---GFTPLHIAAHYGHENV 242 HSP 13 Score: 61.2326 bits (147), Expect = 7.743e-10 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 + G ++H+ V VA+I+ + G+ I +T G P+H A G L ++ L+E D+ +T + P+H A+ GH+ ++ LLE + + G P+ A G++ +++ L Sbjct: 687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 14 Score: 58.5362 bits (140), Expect = 5.763e-9 Identity = 32/152 (21.05%), Postives = 72/152 (47.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 G +H++ G E++ ++I+ GS++ + G+ +H + + + +L +I +TN+G P+H A G ++K L+E ++ + P+H A+ GH ++ LL+ + + ++ L +A + + V+ L Sbjct: 656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 80.4925 bits (197), Expect = 5.781e-16 Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L++ + + +HIA G EVA I++D ++ T G P+H AS G LE++ LLLER ++ E + + P+H A+ + ++ LLLE + + G P+H A+ ++I LLQ K + K+ + L L+ Q + LLI Sbjct: 502 GNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLI 676 HSP 2 Score: 78.5666 bits (192), Expect = 2.165e-15 Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G V VA++++D ++ G P+H A + ++++ LLL+ IE T SG+ P+H A+ G I+ LL++ N + E RG P+H A+ ++++L++ ++D + ++ L +A + D V LL+ Sbjct: 363 LHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511 HSP 3 Score: 77.411 bits (189), Expect = 5.858e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A +V R++I G+ ++ + P+H AS G +I+ LLL+ + T P+H A+ G E+ +LL+ N + +G P+H AS G+L++++LLL+R +D + + L +A LL+ Sbjct: 457 RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLL 610 HSP 4 Score: 76.6406 bits (187), Expect = 9.586e-15 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G V+ AR ++ + ++ T + P+H A+ G + + LLL+R+ D +G P+H A ++++LLL+ IE G+ P+H A+ G + I+ LLQ+ D + E+ L LA ++ Q D V++LI Sbjct: 326 GLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478 HSP 5 Score: 75.485 bits (184), Expect = 2.014e-14 Identity = 41/162 (25.31%), Postives = 82/162 (50.62%), Query Frame = 1 Query: 172 TIVDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T VD +G + H+A +VA ++++ G++ + G P+H A+ + +EI + LL+ D ++ +G P+H ++ GH EI LL+E ++ + N G+ +H + H+ + ++L I+ K N + L +A Q++ V+ L+ Sbjct: 581 TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLV 742 HSP 6 Score: 75.0998 bits (183), Expect = 2.657e-14 Identity = 51/209 (24.40%), Postives = 93/209 (44.50%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA A +++ N + + G P+H A+ G + LLLE+ ++ + + P+H A+ G + + LLL R I+ + P+HCA+ GH Q++ LL+ + I K + L +A Q +D + L+ + D + + D++ + LHV A C R+ K +LD++ D Sbjct: 197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------------TVDYL-----TPLHV---AAHCGHVRVAKLLLDRSAD 384 HSP 7 Score: 73.9442 bits (180), Expect = 7.826e-14 Identity = 39/153 (25.49%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ + IE T G+ P+H A+ G + I+ LL++ + + ET G P+H A+ ++++++L+ ++ + P+H AS G+ I+ LLLQ + + S L +A + Q + +L+ Sbjct: 392 GFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILL 544 HSP 8 Score: 73.559 bits (179), Expect = 8.405e-14 Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G V +++++ G+N+ + + P+H A+ G + LLL R I+ T + P+H A+ GH +++ LL+ + I + G+ P+H A+ G H+ + LL + +D Sbjct: 222 VTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVD 353 HSP 9 Score: 73.559 bits (179), Expect = 9.271e-14 Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A ++ ++ ++ G P+H ++ G EI LL+E D+ + N+G+ +H + H + ++L I + N G P+H A G L ++K L++ + K S + L A Q +CV+ L+ Sbjct: 623 GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLL 775 HSP 10 Score: 73.559 bits (179), Expect = 9.438e-14 Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 +HIA G ++ +++ G+N T P+H A+ G E+ +LL+ N D T G P+H AS G+ E+++LLLER ++ E + P+H A+ + ++ LLL+ + L +A + QM+ L+ + Sbjct: 495 LHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK 646 HSP 11 Score: 72.4034 bits (176), Expect = 2.427e-13 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 KEG + L + T++ +G +H+A G +EV R++++ G+ ++ E + P+H A+ ++ LLLE + +G P+H A+ EI LL+ + + G P+H ++ GH +I LL++ + K N + + L Q + Q+L Sbjct: 533 KEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQIL 708 HSP 12 Score: 69.3218 bits (168), Expect = 2.065e-12 Identity = 50/213 (23.47%), Postives = 98/213 (46.01%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + G+ + V + L++ + G S+H+A G EV R +I + ++ T G +H AS G I+T+L+E ++ ++ +G P++ A+ H E++K LL+ N G P+ A GH +++ +LL+ D K + L +A + LL+ + D +++ F + ++ + EN+ Sbjct: 37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKS---GFTPLHIAAHYGHENV 242 HSP 13 Score: 61.2326 bits (147), Expect = 7.758e-10 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 + G ++H+ V VA+I+ + G+ I +T G P+H A G L ++ L+E D+ +T + P+H A+ GH+ ++ LLE + + G P+ A G++ +++ L Sbjct: 687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 14 Score: 58.5362 bits (140), Expect = 5.774e-9 Identity = 32/152 (21.05%), Postives = 72/152 (47.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 G +H++ G E++ ++I+ GS++ + G+ +H + + + +L +I +TN+G P+H A G ++K L+E ++ + P+H A+ GH ++ LL+ + + ++ L +A + + V+ L Sbjct: 656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 80.4925 bits (197), Expect = 5.781e-16 Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L++ + + +HIA G EVA I++D ++ T G P+H AS G LE++ LLLER ++ E + + P+H A+ + ++ LLLE + + G P+H A+ ++I LLQ K + K+ + L L+ Q + LLI Sbjct: 502 GNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLI 676 HSP 2 Score: 78.5666 bits (192), Expect = 2.165e-15 Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G V VA++++D ++ G P+H A + ++++ LLL+ IE T SG+ P+H A+ G I+ LL++ N + E RG P+H A+ ++++L++ ++D + ++ L +A + D V LL+ Sbjct: 363 LHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511 HSP 3 Score: 77.411 bits (189), Expect = 5.858e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A +V R++I G+ ++ + P+H AS G +I+ LLL+ + T P+H A+ G E+ +LL+ N + +G P+H AS G+L++++LLL+R +D + + L +A LL+ Sbjct: 457 RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLL 610 HSP 4 Score: 76.6406 bits (187), Expect = 9.586e-15 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G V+ AR ++ + ++ T + P+H A+ G + + LLL+R+ D +G P+H A ++++LLL+ IE G+ P+H A+ G + I+ LLQ+ D + E+ L LA ++ Q D V++LI Sbjct: 326 GLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478 HSP 5 Score: 75.485 bits (184), Expect = 2.014e-14 Identity = 41/162 (25.31%), Postives = 82/162 (50.62%), Query Frame = 1 Query: 172 TIVDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T VD +G + H+A +VA ++++ G++ + G P+H A+ + +EI + LL+ D ++ +G P+H ++ GH EI LL+E ++ + N G+ +H + H+ + ++L I+ K N + L +A Q++ V+ L+ Sbjct: 581 TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLV 742 HSP 6 Score: 75.0998 bits (183), Expect = 2.657e-14 Identity = 51/209 (24.40%), Postives = 93/209 (44.50%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA A +++ N + + G P+H A+ G + LLLE+ ++ + + P+H A+ G + + LLL R I+ + P+HCA+ GH Q++ LL+ + I K + L +A Q +D + L+ + D + + D++ + LHV A C R+ K +LD++ D Sbjct: 197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------------TVDYL-----TPLHV---AAHCGHVRVAKLLLDRSAD 384 HSP 7 Score: 73.9442 bits (180), Expect = 7.826e-14 Identity = 39/153 (25.49%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ + IE T G+ P+H A+ G + I+ LL++ + + ET G P+H A+ ++++++L+ ++ + P+H AS G+ I+ LLLQ + + S L +A + Q + +L+ Sbjct: 392 GFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILL 544 HSP 8 Score: 73.559 bits (179), Expect = 8.405e-14 Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G V +++++ G+N+ + + P+H A+ G + LLL R I+ T + P+H A+ GH +++ LL+ + I + G+ P+H A+ G H+ + LL + +D Sbjct: 222 VTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVD 353 HSP 9 Score: 73.559 bits (179), Expect = 9.271e-14 Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A ++ ++ ++ G P+H ++ G EI LL+E D+ + N+G+ +H + H + ++L I + N G P+H A G L ++K L++ + K S + L A Q +CV+ L+ Sbjct: 623 GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLL 775 HSP 10 Score: 73.559 bits (179), Expect = 9.438e-14 Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 +HIA G ++ +++ G+N T P+H A+ G E+ +LL+ N D T G P+H AS G+ E+++LLLER ++ E + P+H A+ + ++ LLL+ + L +A + QM+ L+ + Sbjct: 495 LHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK 646 HSP 11 Score: 72.4034 bits (176), Expect = 2.427e-13 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 KEG + L + T++ +G +H+A G +EV R++++ G+ ++ E + P+H A+ ++ LLLE + +G P+H A+ EI LL+ + + G P+H ++ GH +I LL++ + K N + + L Q + Q+L Sbjct: 533 KEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQIL 708 HSP 12 Score: 69.3218 bits (168), Expect = 2.065e-12 Identity = 50/213 (23.47%), Postives = 98/213 (46.01%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + G+ + V + L++ + G S+H+A G EV R +I + ++ T G +H AS G I+T+L+E ++ ++ +G P++ A+ H E++K LL+ N G P+ A GH +++ +LL+ D K + L +A + LL+ + D +++ F + ++ + EN+ Sbjct: 37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKS---GFTPLHIAAHYGHENV 242 HSP 13 Score: 61.2326 bits (147), Expect = 7.758e-10 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 + G ++H+ V VA+I+ + G+ I +T G P+H A G L ++ L+E D+ +T + P+H A+ GH+ ++ LLE + + G P+ A G++ +++ L Sbjct: 687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 14 Score: 58.5362 bits (140), Expect = 5.774e-9 Identity = 32/152 (21.05%), Postives = 72/152 (47.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 G +H++ G E++ ++I+ GS++ + G+ +H + + + +L +I +TN+G P+H A G ++K L+E ++ + P+H A+ GH ++ LL+ + + ++ L +A + + V+ L Sbjct: 656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Match: unc-44 (AO49 ankyrin [Source:UniProtKB/TrEMBL;Acc:Q7JP86]) HSP 1 Score: 80.4925 bits (197), Expect = 5.794e-16 Identity = 52/175 (29.71%), Postives = 84/175 (48.00%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L++ + + +HIA G EVA I++D ++ T G P+H AS G LE++ LLLER ++ E + + P+H A+ + ++ LLLE + + G P+H A+ ++I LLQ K + K+ + L L+ Q + LLI Sbjct: 502 GNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLI 676 HSP 2 Score: 78.5666 bits (192), Expect = 2.170e-15 Identity = 41/149 (27.52%), Postives = 79/149 (53.02%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G V VA++++D ++ G P+H A + ++++ LLL+ IE T SG+ P+H A+ G I+ LL++ N + E RG P+H A+ ++++L++ ++D + ++ L +A + D V LL+ Sbjct: 363 LHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLL 511 HSP 3 Score: 77.411 bits (189), Expect = 5.871e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A +V R++I G+ ++ + P+H AS G +I+ LLL+ + T P+H A+ G E+ +LL+ N + +G P+H AS G+L++++LLL+R +D + + L +A LL+ Sbjct: 457 RGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLL 610 HSP 4 Score: 76.6406 bits (187), Expect = 9.608e-15 Identity = 45/153 (29.41%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G V+ AR ++ + ++ T + P+H A+ G + + LLL+R+ D +G P+H A ++++LLL+ IE G+ P+H A+ G + I+ LLQ+ D + E+ L LA ++ Q D V++LI Sbjct: 326 GLAPLHMAAQGDHVDAARTLLYHRAPVDDVTVDYLTPLHVAAHCGHVRVAKLLLDRSADPNSRALNGFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLI 478 HSP 5 Score: 75.485 bits (184), Expect = 2.019e-14 Identity = 41/162 (25.31%), Postives = 82/162 (50.62%), Query Frame = 1 Query: 172 TIVDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T VD +G + H+A +VA ++++ G++ + G P+H A+ + +EI + LL+ D ++ +G P+H ++ GH EI LL+E ++ + N G+ +H + H+ + ++L I+ K N + L +A Q++ V+ L+ Sbjct: 581 TPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLV 742 HSP 6 Score: 75.0998 bits (183), Expect = 2.663e-14 Identity = 51/209 (24.40%), Postives = 93/209 (44.50%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA A +++ N + + G P+H A+ G + LLLE+ ++ + + P+H A+ G + + LLL R I+ + P+HCA+ GH Q++ LL+ + I K + L +A Q +D + L+ + D + + D++ + LHV A C R+ K +LD++ D Sbjct: 197 ALHIAAKKDDTTAATLLLQNEHNPDVTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVDDV-------------TVDYL-----TPLHV---AAHCGHVRVAKLLLDRSAD 384 HSP 7 Score: 73.9442 bits (180), Expect = 7.843e-14 Identity = 39/153 (25.49%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ + IE T G+ P+H A+ G + I+ LL++ + + ET G P+H A+ ++++++L+ ++ + P+H AS G+ I+ LLLQ + + S L +A + Q + +L+ Sbjct: 392 GFTPLHIACKKNRIKVVELLLKYRAAIEATTESGLTPLHVAAFMGAINIVIYLLQQGANPDVETVRGETPLHLAARANQTDVVRVLIRNGAKVDAQARELQTPLHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILL 544 HSP 8 Score: 73.559 bits (179), Expect = 8.423e-14 Identity = 35/132 (26.52%), Postives = 66/132 (50.00%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G V +++++ G+N+ + + P+H A+ G + LLL R I+ T + P+H A+ GH +++ LL+ + I + G+ P+H A+ G H+ + LL + +D Sbjct: 222 VTSKSGFTPLHIAAHYGHENVGQLLLEKGANVNYQARHNISPLHVATKWGRTNMANLLLSRGAIIDSRTKDLLTPLHCAARSGHDQVVDLLVVQGAPISAKTKNGLAPLHMAAQGDHVDAARTLLYHRAPVD 353 HSP 9 Score: 73.559 bits (179), Expect = 9.291e-14 Identity = 39/153 (25.49%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A ++ ++ ++ G P+H ++ G EI LL+E D+ + N+G+ +H + H + ++L I + N G P+H A G L ++K L++ + K S + L A Q +CV+ L+ Sbjct: 623 GYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLL 775 HSP 10 Score: 73.559 bits (179), Expect = 9.458e-14 Identity = 41/152 (26.97%), Postives = 73/152 (48.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 +HIA G ++ +++ G+N T P+H A+ G E+ +LL+ N D T G P+H AS G+ E+++LLLER ++ E + P+H A+ + ++ LLL+ + L +A + QM+ L+ + Sbjct: 495 LHIASRLGNTDIVILLLQAGANSNATTRDNYSPLHIAAKEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFK 646 HSP 11 Score: 72.4034 bits (176), Expect = 2.432e-13 Identity = 43/176 (24.43%), Postives = 81/176 (46.02%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 KEG + L + T++ +G +H+A G +EV R++++ G+ ++ E + P+H A+ ++ LLLE + +G P+H A+ EI LL+ + + G P+H ++ GH +I LL++ + K N + + L Q + Q+L Sbjct: 533 KEGQEEVAGILLDHNADKTLLTKKGFTPLHLASKYGNLEVVRLLLERGTPVDIEGKNQVTPLHVAAHYNNDKVAMLLLENGASAKAAAKNGYTPLHIAAKKNQMEIASTLLQFKADPNAKSRAGFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQIL 708 HSP 12 Score: 69.3218 bits (168), Expect = 2.069e-12 Identity = 50/213 (23.47%), Postives = 98/213 (46.01%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + G+ + V + L++ + G S+H+A G EV R +I + ++ T G +H AS G I+T+L+E ++ ++ +G P++ A+ H E++K LL+ N G P+ A GH +++ +LL+ D K + L +A + LL+ + D +++ F + ++ + EN+ Sbjct: 37 FLRAARAGDLEKVLELLRAGTDINTSNANGLNSLHLASKEGHSEVVRELIKRQAQVDAATRKGNTALHIASLAGQSLIVTILVENGANVNVQSVNGFTPLYMAAQENHEEVVKYLLKHGANQALSTEDGFTPLAVALQQGHDRVVAVLLEN----DSKGKVRLPALHIAAKKDDTTAATLLLQNEHNPDVTSKS---GFTPLHIAAHYGHENV 242 HSP 13 Score: 61.2326 bits (147), Expect = 7.772e-10 Identity = 32/121 (26.45%), Postives = 61/121 (50.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 + G ++H+ V VA+I+ + G+ I +T G P+H A G L ++ L+E D+ +T + P+H A+ GH+ ++ LLE + + G P+ A G++ +++ L Sbjct: 687 NNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807 HSP 14 Score: 58.5362 bits (140), Expect = 5.784e-9 Identity = 32/152 (21.05%), Postives = 72/152 (47.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 G +H++ G E++ ++I+ GS++ + G+ +H + + + +L +I +TN+G P+H A G ++K L+E ++ + P+H A+ GH ++ LL+ + + ++ L +A + + V+ L Sbjct: 656 GFTPLHLSAQEGHKEISGLLIENGSDVGAKANNGLTAMHLCAQEDHVPVAQILYNNGAEINSKTNAGYTPLHVACHFGQLNMVKFLVENGADVGEKTRASYTPLHQAAQQGHNNCVRYLLENGASPNEQTATGQTPLSIAQRLGYVSVVETL 807
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303120) HSP 1 Score: 105.145 bits (261), Expect = 3.514e-24 Identity = 59/209 (28.23%), Postives = 101/209 (48.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA V+ A +++D N + + G P+H AS G I LL+++ D+ + P+H A+ G + ++ LLLE+ NIE + G+ P+HCA+ GH Q++ +LL+R I K + L +A Q + +D ++L+ + DE+ D + + LHV A C R+ K +LD+ D Sbjct: 325 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPV------DEVTVDYL------------TALHV---AAHCGHVRVAKLLLDRNAD 512 HSP 2 Score: 90.1225 bits (222), Expect = 4.020e-19 Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L +D + G +HIA G +A ++I G+++ + P+H A+ G +++LLLE+ +IE +T G+ P+H A+ GH +++ +LLER I + G+ P+H A+ G H+ ++LL + +D Sbjct: 342 LDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVD 481 HSP 3 Score: 83.1889 bits (204), Expect = 7.272e-17 Identity = 46/163 (28.22%), Postives = 86/163 (52.76%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFIN 678 G +HIAC ++V +++ G++I T G+ P+H A+ GC+ I+ LL+ + + T G P+H A+ ++I+++LL ++ P+H AS G++ I+ LLLQ ++D + L +A + Q D V+ LI+ +++ D I+ Sbjct: 520 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQ-DEVKDLIA-KKITDHID 680 HSP 4 Score: 80.4925 bits (197), Expect = 5.510e-16 Identity = 41/150 (27.33%), Postives = 78/150 (52.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V VA++++D ++ G P+H A + L+++ LLL I T SG+ P+H A+ G I+ LL+ + + + RG P+H A+ I+++LL+ ++D + ++ L +A + +D V LL+ Sbjct: 490 ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 639 HSP 5 Score: 78.5666 bits (192), Expect = 2.190e-15 Identity = 50/214 (23.36%), Postives = 99/214 (46.26%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + GN + V ++LK++ + G ++H+A G + V ++ G+ ++ T G +H AS G E++ LLLE N + ++ +G P++ A+ H +++LLL N G P+ A GH +++ +LL+ D + + L +A + + LL+ D +++ F + +S + +NI Sbjct: 164 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLES----DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKS---GFTPLHIASHYGNQNI 370 HSP 6 Score: 77.411 bits (189), Expect = 5.435e-15 Identity = 53/215 (24.65%), Postives = 89/215 (41.40%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNC-------------NIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++ G NIE +T G+ P+H A+ G +++ +LLER I +T +G+ P+H A+ G GH + KLLL+RN +I C+ NR G+ P+H A+ G + I+ LLQ D E+ L LA ++ Q D +++L+ Sbjct: 392 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 606
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334062) HSP 1 Score: 104.375 bits (259), Expect = 5.052e-24 Identity = 59/209 (28.23%), Postives = 101/209 (48.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA V+ A +++D N + + G P+H AS G I LL+++ D+ + P+H A+ G + ++ LLLE+ NIE + G+ P+HCA+ GH Q++ +LL+R I K + L +A Q + +D ++L+ + DE+ D + + LHV A C R+ K +LD+ D Sbjct: 175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPV------DEVTVDYL------------TALHV---AAHCGHVRVAKLLLDRNAD 362 HSP 2 Score: 90.5077 bits (223), Expect = 2.409e-19 Identity = 39/132 (29.55%), Postives = 72/132 (54.55%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G +A ++I G+++ + P+H A+ G +++LLLE+ +IE +T G+ P+H A+ GH +++ +LLER I + G+ P+H A+ G H+ ++LL + +D Sbjct: 200 VTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVD 331 HSP 3 Score: 84.3445 bits (207), Expect = 2.121e-17 Identity = 46/163 (28.22%), Postives = 86/163 (52.76%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFIN 678 G +HIAC ++V +++ G++I T G+ P+H A+ GC+ I+ LL+ + + T G P+H A+ ++I+++LL ++ P+H AS G++ I+ LLLQ ++D + L +A + Q D V+ LI+ +++ D I+ Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQ-DEVKDLIA-KKITDHID 530 HSP 4 Score: 81.2629 bits (199), Expect = 2.112e-16 Identity = 41/150 (27.33%), Postives = 78/150 (52.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V VA++++D ++ G P+H A + L+++ LLL I T SG+ P+H A+ G I+ LL+ + + + RG P+H A+ I+++LL+ ++D + ++ L +A + +D V LL+ Sbjct: 340 ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 489 HSP 5 Score: 79.337 bits (194), Expect = 9.502e-16 Identity = 50/214 (23.36%), Postives = 99/214 (46.26%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + GN + V ++LK++ + G ++H+A G + V ++ G+ ++ T G +H AS G E++ LLLE N + ++ +G P++ A+ H +++LLL N G P+ A GH +++ +LL+ D + + L +A + + LL+ D +++ F + +S + +NI Sbjct: 14 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLES----DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKS---GFTPLHIASHYGNQNI 220 HSP 6 Score: 77.411 bits (189), Expect = 3.857e-15 Identity = 53/215 (24.65%), Postives = 89/215 (41.40%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNC-------------NIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++ G NIE +T G+ P+H A+ G +++ +LLER I +T +G+ P+H A+ G GH + KLLL+RN +I C+ NR G+ P+H A+ G + I+ LLQ D E+ L LA ++ Q D +++L+ Sbjct: 242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303119) HSP 1 Score: 104.375 bits (259), Expect = 9.283e-24 Identity = 59/209 (28.23%), Postives = 101/209 (48.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA V+ A +++D N + + G P+H AS G I LL+++ D+ + P+H A+ G + ++ LLLE+ NIE + G+ P+HCA+ GH Q++ +LL+R I K + L +A Q + +D ++L+ + DE+ D + + LHV A C R+ K +LD+ D Sbjct: 175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPV------DEVTVDYL------------TALHV---AAHCGHVRVAKLLLDRNAD 362 HSP 2 Score: 90.5077 bits (223), Expect = 3.743e-19 Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L +D + G +HIA G +A ++I G+++ + P+H A+ G +++LLLE+ +IE +T G+ P+H A+ GH +++ +LLER I + G+ P+H A+ G H+ ++LL + +D Sbjct: 192 LDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVD 331 HSP 3 Score: 85.8853 bits (211), Expect = 1.078e-17 Identity = 42/153 (27.45%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I T G+ P+H A+ GC+ I+ LL+ + + T G P+H A+ ++I+++LL ++ P+H AS G++ I+ LLLQ ++D + L +A + Q + +LI Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI 522 HSP 4 Score: 80.8777 bits (198), Expect = 5.014e-16 Identity = 41/150 (27.33%), Postives = 78/150 (52.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V VA++++D ++ G P+H A + L+++ LLL I T SG+ P+H A+ G I+ LL+ + + + RG P+H A+ I+++LL+ ++D + ++ L +A + +D V LL+ Sbjct: 340 ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 489 HSP 5 Score: 79.337 bits (194), Expect = 1.437e-15 Identity = 50/214 (23.36%), Postives = 99/214 (46.26%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENI 741 FL+ + GN + V ++LK++ + G ++H+A G + V ++ G+ ++ T G +H AS G E++ LLLE N + ++ +G P++ A+ H +++LLL N G P+ A GH +++ +LL+ D + + L +A + + LL+ D +++ F + +S + +NI Sbjct: 14 SFLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLES----DTRGKVRLPALHIAAKKDDVKAATLLLDNDHNPDVTSKS---GFTPLHIASHYGNQNI 220 HSP 6 Score: 78.5666 bits (192), Expect = 2.273e-15 Identity = 39/170 (22.94%), Postives = 79/170 (46.47%), Query Frame = 1 Query: 139 VWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V L+ D + +G +H+A ++ RI++ G+ ++ P+H AS G ++I+ LLL+ ++ T +H A+ G E+ +L+E ++ +G P+H + GH+++ +LLLQ++ +D + + L +A LL+ Sbjct: 419 VIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLL 588 HSP 7 Score: 78.5666 bits (192), Expect = 2.820e-15 Identity = 45/187 (24.06%), Postives = 86/187 (45.99%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 GN D V L+ + ++HIA G EVA ++I+ G+ ++ T G P+H + G +++ LLL++ D++ + +G+ P+H A + ++ LLLE+ + G P+H A+ + I LL+ + ++ + L L+ Q + LLI + + +N Sbjct: 480 GNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKN 666 HSP 8 Score: 77.0258 bits (188), Expect = 7.171e-15 Identity = 53/215 (24.65%), Postives = 89/215 (41.40%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNC-------------NIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++ G NIE +T G+ P+H A+ G +++ +LLER I +T +G+ P+H A+ G GH + KLLL+RN +I C+ NR G+ P+H A+ G + I+ LLQ D E+ L LA ++ Q D +++L+ Sbjct: 242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456 HSP 9 Score: 77.0258 bits (188), Expect = 8.973e-15 Identity = 39/153 (25.49%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GH +HIA +++A +++ G+ E+ G P+H +S G EI LL+E + +G+ P+H + + + ++L + NI+ G P+H AS G +++ LLQ +D ++ + L Q V LL+ Sbjct: 601 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLL 753 HSP 10 Score: 76.6406 bits (187), Expect = 1.143e-14 Identity = 41/173 (23.70%), Postives = 80/173 (46.24%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSS 720 +HIA G V++ +++ G+ ++ T +H A+ G E+ +L+E ++ T G P+H + GH ++ +LLL++ +++ + G+ P+H A + Q+ LLL++ + L +A + QMD L+ E N + F + SS Sbjct: 473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLL---EYGALANAESKAGFTPLHLSS 642 HSP 11 Score: 76.2554 bits (186), Expect = 1.355e-14 Identity = 42/160 (26.25%), Postives = 77/160 (48.12%), Query Frame = 1 Query: 178 VDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 VD QG + H+AC +VA ++++ G++ G P+H A+ + ++I T LLE E+ +G P+H +S GH+EI LL+E + G+ P+H + ++ + ++L + ID + L +A Q + V+ L+ Sbjct: 561 VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL 720 HSP 12 Score: 70.8626 bits (172), Expect = 8.090e-13 Identity = 36/116 (31.03%), Postives = 57/116 (49.14%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQIL 537 G +H+ V VA I+ G+NI+ T G P+H AS G ++ LL+ +++ T+ G P+H + GH I+ LLLE N + G P+H A G++ +L Sbjct: 667 GLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIARKLGYISVL 782 HSP 13 Score: 58.151 bits (139), Expect = 9.805e-9 Identity = 33/167 (19.76%), Postives = 71/167 (42.51%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 + G +H+A G + + ++ ++ + T G P+H A+ +I+ +LL ++ P+H AS G+ +I+ LLL+ ++ +H A+ G ++ +L++ +D + L L + + QLL+ + D +N Sbjct: 401 ESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKN 567
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0334061) HSP 1 Score: 100.523 bits (249), Expect = 1.703e-22 Identity = 59/209 (28.23%), Postives = 101/209 (48.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA V+ A +++D N + + G P+H AS G I LL+++ D+ + P+H A+ G + ++ LLLE+ NIE + G+ P+HCA+ GH Q++ +LL+R I K + L +A Q + +D ++L+ + DE+ D + + LHV A C R+ K +LD+ D Sbjct: 325 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPV------DEVTVDYL------------TALHV---AAHCGHVRVAKLLLDRNAD 512 HSP 2 Score: 87.0409 bits (214), Expect = 4.078e-18 Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L +D + G +HIA G +A ++I G+++ + P+H A+ G +++LLLE+ +IE +T G+ P+H A+ GH +++ +LLER I + G+ P+H A+ G H+ ++LL + +D Sbjct: 342 LDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVD 481 HSP 3 Score: 83.5741 bits (205), Expect = 5.946e-17 Identity = 42/153 (27.45%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I T G+ P+H A+ GC+ I+ LL+ + + T G P+H A+ ++I+++LL ++ P+H AS G++ I+ LLLQ ++D + L +A + Q + +LI Sbjct: 520 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI 672 HSP 4 Score: 79.337 bits (194), Expect = 1.344e-15 Identity = 41/150 (27.33%), Postives = 78/150 (52.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V VA++++D ++ G P+H A + L+++ LLL I T SG+ P+H A+ G I+ LL+ + + + RG P+H A+ I+++LL+ ++D + ++ L +A + +D V LL+ Sbjct: 490 ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 639 HSP 5 Score: 75.485 bits (184), Expect = 2.480e-14 Identity = 36/154 (23.38%), Postives = 74/154 (48.05%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A ++ RI++ G+ ++ P+H AS G ++I+ LLL+ ++ T +H A+ G E+ +L+E ++ +G P+H + GH+++ +LLLQ++ +D + + L +A LL+ Sbjct: 585 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLL 738 HSP 6 Score: 75.0998 bits (183), Expect = 3.185e-14 Identity = 41/154 (26.62%), Postives = 77/154 (50.00%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCR 564 FL+ + GN + V ++LK++ + G ++H+A G + V ++ G+ ++ T G +H AS G E++ LLLE N + ++ +G P++ A+ H +++LLL N G P+ A GH +++ +LL+ R Sbjct: 165 FLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTR 318 HSP 7 Score: 74.7146 bits (182), Expect = 4.679e-14 Identity = 53/215 (24.65%), Postives = 89/215 (41.40%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++ G NIE +T G+ P+H A+ G +++ +LLER I +T +G+ P+H A+ G GH + KLLL+RN + I C+ NR G+ P+H A+ G + I+ LLQ D E+ L LA ++ Q D +++L+ Sbjct: 392 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 606 HSP 8 Score: 73.559 bits (179), Expect = 1.009e-13 Identity = 39/153 (25.49%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GH +HIA +++A +++ G+ E+ G P+H +S G EI LL+E + +G+ P+H + + + ++L + NI+ G P+H AS G +++ LLQ +D ++ + L Q V LL+ Sbjct: 751 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLL 903 HSP 9 Score: 73.1738 bits (178), Expect = 1.677e-13 Identity = 44/175 (25.14%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L+ + ++HIA G EVA ++I+ G+ ++ T G P+H + G +++ LLL++ D++ + +G+ P+H A + ++ LLLE+ + G P+H A+ + I LL+ + ++ + L L+ Q + LLI Sbjct: 630 GNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLI 804 HSP 10 Score: 72.7886 bits (177), Expect = 1.952e-13 Identity = 42/160 (26.25%), Postives = 77/160 (48.12%), Query Frame = 1 Query: 178 VDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 VD QG + H+AC +VA ++++ G++ G P+H A+ + ++I T LLE E+ +G P+H +S GH+EI LL+E + G+ P+H + ++ + ++L + ID + L +A Q + V+ L+ Sbjct: 711 VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL 870 HSP 11 Score: 71.2478 bits (173), Expect = 6.730e-13 Identity = 36/149 (24.16%), Postives = 73/149 (48.99%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G V++ +++ G+ ++ T +H A+ G E+ +L+E ++ T G P+H + GH ++ +LLL++ +++ + G+ P+H A + Q+ LLL++ + L +A + QMD L+ Sbjct: 623 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLL 771 HSP 12 Score: 70.0922 bits (170), Expect = 1.254e-12 Identity = 38/169 (22.49%), Postives = 84/169 (49.70%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIA 696 G +H++ G E++ ++I+ + + G+ P+H + + + +L + +I+ T +G P+H AS G + +++ LL+ N++ + G P+H + GH I+ LLL+ K + + ++ L +A +++ + +L S++ I + DE A Sbjct: 784 GFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA---RKLGYISVLDSLKT----ITKEDETA 945
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Match: Ank2 (gene:FBgn0261788 transcript:FBtr0303118) HSP 1 Score: 100.523 bits (249), Expect = 1.918e-22 Identity = 59/209 (28.23%), Postives = 101/209 (48.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA V+ A +++D N + + G P+H AS G I LL+++ D+ + P+H A+ G + ++ LLLE+ NIE + G+ P+HCA+ GH Q++ +LL+R I K + L +A Q + +D ++L+ + DE+ D + + LHV A C R+ K +LD+ D Sbjct: 175 ALHIAAKKDDVKAATLLLDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPV------DEVTVDYL------------TALHV---AAHCGHVRVAKLLLDRNAD 362 HSP 2 Score: 87.0409 bits (214), Expect = 4.559e-18 Identity = 41/140 (29.29%), Postives = 75/140 (53.57%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L +D + G +HIA G +A ++I G+++ + P+H A+ G +++LLLE+ +IE +T G+ P+H A+ GH +++ +LLER I + G+ P+H A+ G H+ ++LL + +D Sbjct: 192 LDNDHNPDVTSKSGFTPLHIASHYGNQNIANLLIQKGADVNYSAKHNISPLHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVD 331 HSP 3 Score: 83.5741 bits (205), Expect = 6.632e-17 Identity = 42/153 (27.45%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I T G+ P+H A+ GC+ I+ LL+ + + T G P+H A+ ++I+++LL ++ P+H AS G++ I+ LLLQ ++D + L +A + Q + +LI Sbjct: 370 GFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLI 522 HSP 4 Score: 79.337 bits (194), Expect = 1.494e-15 Identity = 41/150 (27.33%), Postives = 78/150 (52.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V VA++++D ++ G P+H A + L+++ LLL I T SG+ P+H A+ G I+ LL+ + + + RG P+H A+ I+++LL+ ++D + ++ L +A + +D V LL+ Sbjct: 340 ALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLL 489 HSP 5 Score: 75.485 bits (184), Expect = 2.747e-14 Identity = 36/154 (23.38%), Postives = 74/154 (48.05%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G +H+A ++ RI++ G+ ++ P+H AS G ++I+ LLL+ ++ T +H A+ G E+ +L+E ++ +G P+H + GH+++ +LLLQ++ +D + + L +A LL+ Sbjct: 435 RGETPLHLAARANQTDIIRILLRNGAQVDARAREQQTPLHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLL 588 HSP 6 Score: 75.0998 bits (183), Expect = 3.527e-14 Identity = 41/154 (26.62%), Postives = 77/154 (50.00%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCR 564 FL+ + GN + V ++LK++ + G ++H+A G + V ++ G+ ++ T G +H AS G E++ LLLE N + ++ +G P++ A+ H +++LLL N G P+ A GH +++ +LL+ R Sbjct: 15 FLRAARAGNLERVLEHLKNNIDINTSNANGLNALHLASKDGHIHVVSELLRRGAIVDSATKKGNTALHIASLAGQEEVVKLLLEHNASVNVQSQNGFTPLYMAAQENHDAVVRLLLSNGANQSLATEDGFTPLAVAMQQGHDKVVAVLLESDTR 168 HSP 7 Score: 74.7146 bits (182), Expect = 5.178e-14 Identity = 53/215 (24.65%), Postives = 89/215 (41.40%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++ G NIE +T G+ P+H A+ G +++ +LLER I +T +G+ P+H A+ G GH + KLLL+RN + I C+ NR G+ P+H A+ G + I+ LLQ D E+ L LA ++ Q D +++L+ Sbjct: 242 LHVAAKWGKTNMVSLLLEKGGNIEAKTRDGLTPLHCAARSGHEQVVDMLLERGAPISAKTKNGLAPLHMAAQGEHVDAARILLYHRAPVDEVTVDYLTALHVAAHCGHVRVAKLLLDRNADANARALNGFTPLHIACKKNRLKVVELLLRHGASISATTESGLTPLHVAAFMGCMNIVIYLLQHDASPDVPTVRGETPLHLAARANQTDIIRILL 456 HSP 8 Score: 73.559 bits (179), Expect = 1.115e-13 Identity = 39/153 (25.49%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GH +HIA +++A +++ G+ E+ G P+H +S G EI LL+E + +G+ P+H + + + ++L + NI+ G P+H AS G +++ LLQ +D ++ + L Q V LL+ Sbjct: 601 GHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLL 753 HSP 9 Score: 73.1738 bits (178), Expect = 1.853e-13 Identity = 44/175 (25.14%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN D V L+ + ++HIA G EVA ++I+ G+ ++ T G P+H + G +++ LLL++ D++ + +G+ P+H A + ++ LLLE+ + G P+H A+ + I LL+ + ++ + L L+ Q + LLI Sbjct: 480 GNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLI 654 HSP 10 Score: 72.7886 bits (177), Expect = 2.156e-13 Identity = 42/160 (26.25%), Postives = 77/160 (48.12%), Query Frame = 1 Query: 178 VDDQGHYSI---HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 VD QG + H+AC +VA ++++ G++ G P+H A+ + ++I T LLE E+ +G P+H +S GH+EI LL+E + G+ P+H + ++ + ++L + ID + L +A Q + V+ L+ Sbjct: 561 VDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLLEYGALANAESKAGFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLL 720 HSP 11 Score: 71.2478 bits (173), Expect = 7.421e-13 Identity = 36/149 (24.16%), Postives = 73/149 (48.99%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G V++ +++ G+ ++ T +H A+ G E+ +L+E ++ T G P+H + GH ++ +LLL++ +++ + G+ P+H A + Q+ LLL++ + L +A + QMD L+ Sbjct: 473 LHIASRLGNVDIVMLLLQHGAQVDATTKDMYTALHIAAKEGQDEVAAVLIENGAALDAATKKGFTPLHLTAKYGHIKVAQLLLQKEADVDAQGKNGVTPLHVACHYNNQQVALLLLEKGASPHATAKNGHTPLHIAARKNQMDIATTLL 621 HSP 12 Score: 70.0922 bits (170), Expect = 1.382e-12 Identity = 38/169 (22.49%), Postives = 84/169 (49.70%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIA 696 G +H++ G E++ ++I+ + + G+ P+H + + + +L + +I+ T +G P+H AS G + +++ LL+ N++ + G P+H + GH I+ LLL+ K + + ++ L +A +++ + +L S++ I + DE A Sbjct: 634 GFTPLHLSSQEGHAEISNLLIEHKAAVNHPAKNGLTPMHLCAQEDNVNVAEILEKNGANIDMATKAGYTPLHVASHFGQANMVRFLLQNGANVDAATSIGYTPLHQTAQQGHCHIVNLLLEHKANANAQTVNGQTPLHIA---RKLGYISVLDSLKT----ITKEDETA 795
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 107.457 bits (267), Expect = 5.051e-25 Identity = 60/206 (29.13%), Postives = 106/206 (51.46%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + + P+H AS G++ +++LLLER I+ G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLLI + E+ D + ++D++ + LHV A C ++ K ++DK Sbjct: 176 ALHIAARKDDTKAAALLLQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLI-----------HHEVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLVDK 360 HSP 2 Score: 93.2041 bits (230), Expect = 3.106e-20 Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D I G +HIA G + VA ++++ G+ ++ + + P+H AS RG ++ LLLER I+ T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LL+ + +D N + L +A ++L+ Sbjct: 193 LQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLV 358 HSP 3 Score: 81.2629 bits (199), Expect = 2.641e-16 Identity = 42/157 (26.75%), Postives = 78/157 (49.68%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD 669 ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++K L+ +D K ++ L ++ + + D VQ L+ L D Sbjct: 341 ALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPD 497 HSP 4 Score: 76.6406 bits (187), Expect = 9.684e-15 Identity = 45/183 (24.59%), Postives = 84/183 (45.90%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + Y+K+ I + G ++H+A G VEV +I G+ ++ T G +H AS G +++ L+ +I ++ +G P++ A+ H +++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 15 SYLRAARAGNIEKALDYIKTGVDINICNQNGLNALHLASKEGHVEVVTELIKLGATVDAATKKGNTALHIASLAGQADVVKELVNNGANINAQSQNGFTPLYMAAQENHLDVVKFLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 193 HSP 5 Score: 75.0998 bits (183), Expect = 3.016e-14 Identity = 36/149 (24.16%), Postives = 81/149 (54.36%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + R++++ G+ I+ T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LL+ ++ N + +H A+ GH ++ K+L+ ++ + K + L +A + ++ ++LL+ Sbjct: 243 LHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIACKKNRIKVMELLL 391 HSP 6 Score: 68.5514 bits (166), Expect = 3.942e-12 Identity = 38/153 (24.84%), Postives = 68/153 (44.44%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G I+T L G +H A+ G + ++K L+ +++ + P+H +S G I++ LLQ D + L LA + D +L+ Sbjct: 371 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILL 523 HSP 7 Score: 55.4546 bits (132), Expect = 7.059e-8 Identity = 28/124 (22.58%), Postives = 54/124 (43.55%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLL 549 V + G IH+A G + + + G++ G +H A+ G ++ L+ D++ + P+H +S G +I++ LL+ + G P+H A+ GH + +LL Sbjct: 400 VTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILL 523 HSP 8 Score: 54.299 bits (129), Expect = 1.404e-7 Identity = 23/94 (24.47%), Postives = 49/94 (52.13%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNI 468 +G ++H+A G V + ++ G++++ + P+H +S G +I+ LL+ + T SG P+H A+ GH ++ +LL+ ++ Sbjct: 436 RGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILLDNGASL 529
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 107.842 bits (268), Expect = 5.264e-25 Identity = 60/206 (29.13%), Postives = 106/206 (51.46%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + + P+H AS G++ +++LLLER I+ G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLLI + E+ D + ++D++ + LHV A C ++ K ++DK Sbjct: 204 ALHIAARKDDTKAAALLLQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLI-----------HHEVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLVDK 388 HSP 2 Score: 93.2041 bits (230), Expect = 3.388e-20 Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D I G +HIA G + VA ++++ G+ ++ + + P+H AS RG ++ LLLER I+ T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LL+ + +D N + L +A ++L+ Sbjct: 221 LQNDHNADIESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLV 386 HSP 3 Score: 81.6481 bits (200), Expect = 2.534e-16 Identity = 42/157 (26.75%), Postives = 78/157 (49.68%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD 669 ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++K L+ +D K ++ L ++ + + D VQ L+ L D Sbjct: 369 ALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPD 525 HSP 4 Score: 76.6406 bits (187), Expect = 9.766e-15 Identity = 45/183 (24.59%), Postives = 84/183 (45.90%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + Y+K+ I + G ++H+A G VEV +I G+ ++ T G +H AS G +++ L+ +I ++ +G P++ A+ H +++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 43 SYLRAARAGNIEKALDYIKTGVDINICNQNGLNALHLASKEGHVEVVTELIKLGATVDAATKKGNTALHIASLAGQADVVKELVNNGANINAQSQNGFTPLYMAAQENHLDVVKFLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 221 HSP 5 Score: 75.0998 bits (183), Expect = 2.878e-14 Identity = 36/149 (24.16%), Postives = 81/149 (54.36%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + R++++ G+ I+ T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LL+ ++ N + +H A+ GH ++ K+L+ ++ + K + L +A + ++ ++LL+ Sbjct: 271 LHVASKRGNANMVRLLLERGARIDARTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLIHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVLVDKRANPNAKALNGFTPLHIACKKNRIKVMELLL 419 HSP 6 Score: 68.5514 bits (166), Expect = 3.746e-12 Identity = 38/153 (24.84%), Postives = 68/153 (44.44%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G I+T L G +H A+ G + ++K L+ +++ + P+H +S G I++ LLQ D + L LA + D +L+ Sbjct: 399 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILL 551 HSP 7 Score: 55.4546 bits (132), Expect = 6.142e-8 Identity = 28/124 (22.58%), Postives = 54/124 (43.55%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLL 549 V + G IH+A G + + + G++ G +H A+ G ++ L+ D++ + P+H +S G +I++ LL+ + G P+H A+ GH + +LL Sbjct: 428 VTESGLTPIHVAAFMGHENIVTQLTNHGASPNTMNVRGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILL 551 HSP 8 Score: 54.6842 bits (130), Expect = 1.148e-7 Identity = 23/94 (24.47%), Postives = 49/94 (52.13%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNI 468 +G ++H+A G V + ++ G++++ + P+H +S G +I+ LL+ + T SG P+H A+ GH ++ +LL+ ++ Sbjct: 464 RGETALHMAARAGQANVVKFLVANGADVDAKAKDDQTPLHISSRLGKPDIVQQLLQHGALPDSTTTSGYTPLHLAARDGHKDVASILLDNGASL 557
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 101.679 bits (252), Expect = 1.031e-22 Identity = 60/209 (28.71%), Postives = 103/209 (49.28%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G+ ++KLLL+R IE + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDV-------------TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 393 HSP 2 Score: 92.0485 bits (227), Expect = 1.609e-19 Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R IE +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 223 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIV 388 HSP 3 Score: 85.5001 bits (210), Expect = 1.843e-17 Identity = 46/163 (28.22%), Postives = 86/163 (52.76%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G + +++ LLQ ++D K ++ L +A + +++ VQ L+ Sbjct: 358 NAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLL 520 HSP 4 Score: 83.1889 bits (204), Expect = 1.055e-16 Identity = 45/154 (29.22%), Postives = 76/154 (49.35%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G ++V R ++ G+ ++ + +H AS G LEI+ LL++ T SG P+H ++ GH EI LLLE+ ++ +G P+H A+ G L++ LLLQ+K D + L +A LL+ Sbjct: 466 RGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLL 619 HSP 5 Score: 82.4185 bits (202), Expect = 2.397e-16 Identity = 50/187 (26.74%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G + V + L+ L G+ +H++ G E+A ++++ GS++ T G P+H A+ G LE+ LLL++ + SG+ P+H A+ + + LLL++ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ Q + N+N Sbjct: 511 GKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKN 697 HSP 6 Score: 81.6481 bits (200), Expect = 4.006e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS IE +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 273 LHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 421 HSP 7 Score: 80.8777 bits (198), Expect = 7.256e-16 Identity = 48/182 (26.37%), Postives = 87/182 (47.80%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + V YLK+ I + G ++H+A G VEV ++ G+N++ T G +H AS G E++ L+ ++ ++ +G P++ A+ H ++++ LLE N + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 46 YLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 223 HSP 8 Score: 73.559 bits (179), Expect = 1.395e-13 Identity = 50/203 (24.63%), Postives = 89/203 (43.84%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q+D V+ L+ D ++D+ A Sbjct: 302 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTAL 504 HSP 9 Score: 73.559 bits (179), Expect = 1.667e-13 Identity = 34/153 (22.22%), Postives = 76/153 (49.67%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL + ++ +G+ P+H A+ + + ++LL I+ + G P+H A G++++ LL+ + + + K + L A Q + +L+ Sbjct: 632 GYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLL 784 HSP 10 Score: 70.8626 bits (172), Expect = 1.110e-12 Identity = 41/156 (26.28%), Postives = 71/156 (45.51%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL + N+ + G+ P+H A+ + ++LL ID + + L +A M L+ Q Sbjct: 599 GLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQ 754 HSP 11 Score: 67.781 bits (164), Expect = 1.066e-11 Identity = 35/125 (28.00%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG +H+A G V++ +++ +N+ G+ P+H A+ + +LL +I+ +T SG P+H A G+ ++ LLE + G P+H A+ GH I+ +LLQ Sbjct: 661 VTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQ 785 HSP 12 Score: 59.3066 bits (142), Expect = 5.337e-9 Identity = 49/236 (20.76%), Postives = 94/236 (39.83%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNE 876 V + G IH+A G + + + G++ G +H A+ G ++++ LL+ ++ + +H AS G EI++ LL++ G P+H ++ GH +I LLL++ + + L +A + Q++ LL+ + D + SGL L +A + R+ +LD+ P T H ++KN+ Sbjct: 430 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGK---------------------SGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQ 644 HSP 13 Score: 59.3066 bits (142), Expect = 5.527e-9 Identity = 36/153 (23.53%), Postives = 69/153 (45.10%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G ++++ LL+ ++ + +H AS G L+I++ LLQ+ + + L L+ + + LL+ Sbjct: 401 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLL 553 HSP 14 Score: 56.6102 bits (135), Expect = 4.354e-8 Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V ++++ G+ I+ +T G P+H A G +++ LLE +T +G P+H A+ GH+ I+ +LL+ Sbjct: 674 QEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQ 785
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 101.679 bits (252), Expect = 1.031e-22 Identity = 60/209 (28.71%), Postives = 103/209 (49.28%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G+ ++KLLL+R IE + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDV-------------TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 393 HSP 2 Score: 92.0485 bits (227), Expect = 1.609e-19 Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R IE +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 223 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIV 388 HSP 3 Score: 85.5001 bits (210), Expect = 1.843e-17 Identity = 46/163 (28.22%), Postives = 86/163 (52.76%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G + +++ LLQ ++D K ++ L +A + +++ VQ L+ Sbjct: 358 NAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLL 520 HSP 4 Score: 83.1889 bits (204), Expect = 1.055e-16 Identity = 45/154 (29.22%), Postives = 76/154 (49.35%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G ++V R ++ G+ ++ + +H AS G LEI+ LL++ T SG P+H ++ GH EI LLLE+ ++ +G P+H A+ G L++ LLLQ+K D + L +A LL+ Sbjct: 466 RGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLL 619 HSP 5 Score: 82.4185 bits (202), Expect = 2.397e-16 Identity = 50/187 (26.74%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G + V + L+ L G+ +H++ G E+A ++++ GS++ T G P+H A+ G LE+ LLL++ + SG+ P+H A+ + + LLL++ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ Q + N+N Sbjct: 511 GKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKN 697 HSP 6 Score: 81.6481 bits (200), Expect = 4.006e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS IE +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 273 LHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 421 HSP 7 Score: 80.8777 bits (198), Expect = 7.256e-16 Identity = 48/182 (26.37%), Postives = 87/182 (47.80%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + V YLK+ I + G ++H+A G VEV ++ G+N++ T G +H AS G E++ L+ ++ ++ +G P++ A+ H ++++ LLE N + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 46 YLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 223 HSP 8 Score: 73.559 bits (179), Expect = 1.395e-13 Identity = 50/203 (24.63%), Postives = 89/203 (43.84%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q+D V+ L+ D ++D+ A Sbjct: 302 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTAL 504 HSP 9 Score: 73.559 bits (179), Expect = 1.667e-13 Identity = 34/153 (22.22%), Postives = 76/153 (49.67%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL + ++ +G+ P+H A+ + + ++LL I+ + G P+H A G++++ LL+ + + + K + L A Q + +L+ Sbjct: 632 GYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLL 784 HSP 10 Score: 70.8626 bits (172), Expect = 1.110e-12 Identity = 41/156 (26.28%), Postives = 71/156 (45.51%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL + N+ + G+ P+H A+ + ++LL ID + + L +A M L+ Q Sbjct: 599 GLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQ 754 HSP 11 Score: 67.781 bits (164), Expect = 1.066e-11 Identity = 35/125 (28.00%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG +H+A G V++ +++ +N+ G+ P+H A+ + +LL +I+ +T SG P+H A G+ ++ LLE + G P+H A+ GH I+ +LLQ Sbjct: 661 VTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQ 785 HSP 12 Score: 59.3066 bits (142), Expect = 5.337e-9 Identity = 49/236 (20.76%), Postives = 94/236 (39.83%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNE 876 V + G IH+A G + + + G++ G +H A+ G ++++ LL+ ++ + +H AS G EI++ LL++ G P+H ++ GH +I LLL++ + + L +A + Q++ LL+ + D + SGL L +A + R+ +LD+ P T H ++KN+ Sbjct: 430 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGK---------------------SGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQ 644 HSP 13 Score: 59.3066 bits (142), Expect = 5.527e-9 Identity = 36/153 (23.53%), Postives = 69/153 (45.10%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G ++++ LL+ ++ + +H AS G L+I++ LLQ+ + + L L+ + + LL+ Sbjct: 401 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLL 553 HSP 14 Score: 56.6102 bits (135), Expect = 4.354e-8 Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V ++++ G+ I+ +T G P+H A G +++ LLE +T +G P+H A+ GH+ I+ +LL+ Sbjct: 674 QEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKNGYTPLHQAAQQGHTHIINMLLQ 785
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Match: BX469921.1 (ankyrin 3a [Source:NCBI gene;Acc:103909035]) HSP 1 Score: 101.679 bits (252), Expect = 1.032e-22 Identity = 60/209 (28.71%), Postives = 103/209 (49.28%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G+ ++KLLL+R IE + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 206 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDV-------------TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 393 HSP 2 Score: 92.0485 bits (227), Expect = 1.610e-19 Identity = 47/166 (28.31%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R IE +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 223 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIV 388 HSP 3 Score: 85.5001 bits (210), Expect = 1.844e-17 Identity = 46/163 (28.22%), Postives = 86/163 (52.76%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G + +++ LLQ ++D K ++ L +A + +++ VQ L+ Sbjct: 358 NAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLL 520 HSP 4 Score: 83.1889 bits (204), Expect = 1.055e-16 Identity = 45/154 (29.22%), Postives = 76/154 (49.35%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G ++V R ++ G+ ++ + +H AS G LEI+ LL++ T SG P+H ++ GH EI LLLE+ ++ +G P+H A+ G L++ LLLQ+K D + L +A LL+ Sbjct: 466 RGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLL 619 HSP 5 Score: 82.4185 bits (202), Expect = 2.398e-16 Identity = 50/187 (26.74%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G + V + L+ L G+ +H++ G E+A ++++ GS++ T G P+H A+ G LE+ LLL++ + SG+ P+H A+ + + LLL++ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ Q + N+N Sbjct: 511 GKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGKSGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKN 697 HSP 6 Score: 81.6481 bits (200), Expect = 4.008e-16 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS IE +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 273 LHVASKRGNGNMVKLLLDRGSKIEAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 421 HSP 7 Score: 80.8777 bits (198), Expect = 7.261e-16 Identity = 48/182 (26.37%), Postives = 87/182 (47.80%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + V YLK+ I + G ++H+A G VEV ++ G+N++ T G +H AS G E++ L+ ++ ++ +G P++ A+ H ++++ LLE N + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 46 YLRAARAGNLEKVLDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGANVDAATKKGNTALHIASLAGQTEVVRELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRFLLENNSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 223 HSP 8 Score: 73.559 bits (179), Expect = 1.396e-13 Identity = 50/203 (24.63%), Postives = 89/203 (43.84%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q+D V+ L+ D ++D+ A Sbjct: 302 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNAPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTAL 504 HSP 9 Score: 70.8626 bits (172), Expect = 1.111e-12 Identity = 41/156 (26.28%), Postives = 71/156 (45.51%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL + N+ + G+ P+H A+ + ++LL ID + + L +A M L+ Q Sbjct: 599 GLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQ 754 HSP 10 Score: 69.707 bits (169), Expect = 2.470e-12 Identity = 30/132 (22.73%), Postives = 69/132 (52.27%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNM 585 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL + ++ +G+ P+H A+ + + ++LL I+ + G P+H A G++++ LL+ + + + K + Sbjct: 632 GYTPLHIAAKKNQLEIGTTLLEYGAECNTVTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKV 763 HSP 11 Score: 59.3066 bits (142), Expect = 5.339e-9 Identity = 49/236 (20.76%), Postives = 94/236 (39.83%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNE 876 V + G IH+A G + + + G++ G +H A+ G ++++ LL+ ++ + +H AS G EI++ LL++ G P+H ++ GH +I LLL++ + + L +A + Q++ LL+ + D + SGL L +A + R+ +LD+ P T H ++KN+ Sbjct: 430 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLLEQGSSLSAATKKGFTPLHVAAKYGQLEVANLLLQKKAAPDAAGK---------------------SGLTPLHVAAHYDNQRVALLLLDQGASPHSPAKNGYTPLHIAAKKNQ 644 HSP 12 Score: 59.3066 bits (142), Expect = 5.530e-9 Identity = 36/153 (23.53%), Postives = 69/153 (45.10%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G ++++ LL+ ++ + +H AS G L+I++ LLQ+ + + L L+ + + LL+ Sbjct: 401 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQIDVVRYLLQNGAKVDIKAKDDQTALHIASRLGKLEIVQQLLQKGALPNAATTSGYTPLHLSAREGHQEIAALLL 553 HSP 13 Score: 50.0618 bits (118), Expect = 4.835e-6 Identity = 32/130 (24.62%), Postives = 59/130 (45.38%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRP-----IHCASSGGHLQILKLLLQ 552 V QG +H+A G V++ +++ +N+ G+ P+H A+ + +LL +I+ +T SG P+H A G+ ++ LLE + + I ++ GH I+ +LLQ Sbjct: 661 VTRQGISPLHLAAQEGSVDLVSLLLTKQANVNMGNKNGLTPLHLAAQDDKAGVTEVLLNHGAEIDAQTKSGYTPLHVACHYGNMKMANFLLENQAKPNAKTKVIVNGYFDMLIPFSAQQGHTHIINMLLQ 790
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097]) HSP 1 Score: 100.908 bits (250), Expect = 2.309e-22 Identity = 59/209 (28.23%), Postives = 104/209 (49.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++ +LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 188 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 375 HSP 2 Score: 91.2781 bits (225), Expect = 4.000e-19 Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++ +LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 83.5741 bits (205), Expect = 1.325e-16 Identity = 41/149 (27.52%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLL 403 HSP 4 Score: 80.1073 bits (196), Expect = 1.666e-15 Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + L+++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++++ LLQ +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 5 Score: 78.1814 bits (191), Expect = 7.737e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ + T SG P+H +S GH ++ +LLE + +G P+H A+ G ++++KLLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.7962 bits (190), Expect = 8.387e-15 Identity = 46/182 (25.27%), Postives = 86/182 (47.25%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VE+ +I G++++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 7 Score: 76.2554 bits (186), Expect = 2.736e-14 Identity = 45/176 (25.57%), Postives = 84/176 (47.73%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G AD V + LK G+ +H++ G +VA ++++ G++ T G P+H A+ G +E++ LLL++N + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL++ Sbjct: 493 GKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLT 668 HSP 8 Score: 74.3294 bits (181), Expect = 1.185e-13 Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ P+H A+ GH +++ LLL R+ N+ + G+ P+H A+ + + ++L + ID M + L +A + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLL 733 HSP 9 Score: 72.7886 bits (177), Expect = 4.512e-13 Identity = 36/153 (23.53%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ P+H A+ G +++++LLL R+ ++ SG+ P+H A+ + ++L + I+ P+H AS G+++++ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 766 HSP 10 Score: 70.0922 bits (170), Expect = 2.532e-12 Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G+ + TT G P+H +S G ++ ++LLE T G P+H A+ G E++KLLL++N + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 481 DDQT--PLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLL 634 HSP 11 Score: 68.5514 bits (166), Expect = 8.843e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L + N E+ L +A ++ Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLL 469 HSP 12 Score: 67.3958 bits (163), Expect = 1.979e-11 Identity = 64/282 (22.70%), Postives = 114/282 (40.43%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRND------EIAFDKIWKSSDFIRENIY------SGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY-------------PVDIAFQCGHL 951 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L G +H A+ G SE+++ LL+ +E + P+H ++ G I++ LL++ D + L L+ + D +L+ F + + + KI +++N SGL L +A + ++ +LDK T H ++KN+ D I LEY PV +A Q GH+ Sbjct: 383 GFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMD----IATTLLEYGADANAITKQGIAPVHLAAQEGHV 660 HSP 13 Score: 63.5438 bits (153), Expect = 3.483e-10 Identity = 31/125 (24.80%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 + QG +H+A G V++ +++ +N+ G+ P+H A+ + + +L + I+ T P+H AS G+ +++ LL+ + + G P+H A+ GH I+ +LLQ Sbjct: 643 ITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 767 HSP 14 Score: 59.3066 bits (142), Expect = 8.145e-9 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V VA ++ + G+ I+ T P+H AS G ++++T LL+ + +T +G P+H A+ GH+ I+ +LL+ Sbjct: 656 QEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 767
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097]) HSP 1 Score: 100.908 bits (250), Expect = 2.315e-22 Identity = 59/209 (28.23%), Postives = 104/209 (49.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++ +LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 179 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 366 HSP 2 Score: 91.2781 bits (225), Expect = 4.011e-19 Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++ +LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 196 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 361 HSP 3 Score: 83.5741 bits (205), Expect = 1.329e-16 Identity = 41/149 (27.52%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 246 LHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLL 394 HSP 4 Score: 80.1073 bits (196), Expect = 1.670e-15 Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + L+++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++++ LLQ +++ K ++ L ++ + + D VQ L+ Sbjct: 331 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLL 493 HSP 5 Score: 78.1814 bits (191), Expect = 7.755e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ + T SG P+H +S GH ++ +LLE + +G P+H A+ G ++++KLLLQ+ D + L +A LL+ Sbjct: 439 RGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLL 592 HSP 6 Score: 77.7962 bits (190), Expect = 8.407e-15 Identity = 46/182 (25.27%), Postives = 86/182 (47.25%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VE+ +I G++++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 19 YLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 196 HSP 7 Score: 76.2554 bits (186), Expect = 2.742e-14 Identity = 45/176 (25.57%), Postives = 84/176 (47.73%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G AD V + LK G+ +H++ G +VA ++++ G++ T G P+H A+ G +E++ LLL++N + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL++ Sbjct: 484 GKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLT 659 HSP 8 Score: 74.3294 bits (181), Expect = 1.188e-13 Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ P+H A+ GH +++ LLL R+ N+ + G+ P+H A+ + + ++L + ID M + L +A + V L+ Sbjct: 572 GLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLL 724 HSP 9 Score: 72.7886 bits (177), Expect = 4.523e-13 Identity = 36/153 (23.53%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ P+H A+ G +++++LLL R+ ++ SG+ P+H A+ + ++L + I+ P+H AS G+++++ LLQ +++ K + L A Q + +L+ Sbjct: 605 GYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 757 HSP 10 Score: 70.0922 bits (170), Expect = 2.538e-12 Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G+ + TT G P+H +S G ++ ++LLE T G P+H A+ G E++KLLL++N + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 472 DDQT--PLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLL 625 HSP 11 Score: 68.5514 bits (166), Expect = 8.862e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L + N E+ L +A ++ Q + V+ L+ Sbjct: 251 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLL 460 HSP 12 Score: 67.3958 bits (163), Expect = 1.983e-11 Identity = 64/282 (22.70%), Postives = 114/282 (40.43%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRND------EIAFDKIWKSSDFIRENIY------SGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY-------------PVDIAFQCGHL 951 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L G +H A+ G SE+++ LL+ +E + P+H ++ G I++ LL++ D + L L+ + D +L+ F + + + KI +++N SGL L +A + ++ +LDK T H ++KN+ D I LEY PV +A Q GH+ Sbjct: 374 GFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMD----IATTLLEYGADANAITKQGIAPVHLAAQEGHV 651 HSP 13 Score: 63.5438 bits (153), Expect = 3.490e-10 Identity = 31/125 (24.80%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 + QG +H+A G V++ +++ +N+ G+ P+H A+ + + +L + I+ T P+H AS G+ +++ LL+ + + G P+H A+ GH I+ +LLQ Sbjct: 634 ITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758 HSP 14 Score: 59.3066 bits (142), Expect = 8.160e-9 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V VA ++ + G+ I+ T P+H AS G ++++T LL+ + +T +G P+H A+ GH+ I+ +LL+ Sbjct: 647 QEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097]) HSP 1 Score: 100.908 bits (250), Expect = 2.319e-22 Identity = 59/209 (28.23%), Postives = 104/209 (49.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++ +LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 179 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 366 HSP 2 Score: 91.2781 bits (225), Expect = 4.017e-19 Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++ +LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 196 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 361 HSP 3 Score: 83.5741 bits (205), Expect = 1.331e-16 Identity = 41/149 (27.52%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 246 LHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLL 394 HSP 4 Score: 80.1073 bits (196), Expect = 1.673e-15 Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + L+++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++++ LLQ +++ K ++ L ++ + + D VQ L+ Sbjct: 331 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLL 493 HSP 5 Score: 78.1814 bits (191), Expect = 7.767e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ + T SG P+H +S GH ++ +LLE + +G P+H A+ G ++++KLLLQ+ D + L +A LL+ Sbjct: 439 RGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLL 592 HSP 6 Score: 77.7962 bits (190), Expect = 8.419e-15 Identity = 46/182 (25.27%), Postives = 86/182 (47.25%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VE+ +I G++++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 19 YLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 196 HSP 7 Score: 76.2554 bits (186), Expect = 2.746e-14 Identity = 45/176 (25.57%), Postives = 84/176 (47.73%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G AD V + LK G+ +H++ G +VA ++++ G++ T G P+H A+ G +E++ LLL++N + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL++ Sbjct: 484 GKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLT 659 HSP 8 Score: 74.3294 bits (181), Expect = 1.189e-13 Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ P+H A+ GH +++ LLL R+ N+ + G+ P+H A+ + + ++L + ID M + L +A + V L+ Sbjct: 572 GLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLL 724 HSP 9 Score: 72.7886 bits (177), Expect = 4.529e-13 Identity = 36/153 (23.53%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ P+H A+ G +++++LLL R+ ++ SG+ P+H A+ + ++L + I+ P+H AS G+++++ LLQ +++ K + L A Q + +L+ Sbjct: 605 GYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 757 HSP 10 Score: 70.0922 bits (170), Expect = 2.542e-12 Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G+ + TT G P+H +S G ++ ++LLE T G P+H A+ G E++KLLL++N + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 472 DDQT--PLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLL 625 HSP 11 Score: 68.5514 bits (166), Expect = 8.874e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L + N E+ L +A ++ Q + V+ L+ Sbjct: 251 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLL 460 HSP 12 Score: 67.3958 bits (163), Expect = 1.986e-11 Identity = 64/282 (22.70%), Postives = 114/282 (40.43%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRND------EIAFDKIWKSSDFIRENIY------SGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY-------------PVDIAFQCGHL 951 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L G +H A+ G SE+++ LL+ +E + P+H ++ G I++ LL++ D + L L+ + D +L+ F + + + KI +++N SGL L +A + ++ +LDK T H ++KN+ D I LEY PV +A Q GH+ Sbjct: 374 GFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMD----IATTLLEYGADANAITKQGIAPVHLAAQEGHV 651 HSP 13 Score: 63.5438 bits (153), Expect = 3.495e-10 Identity = 31/125 (24.80%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 + QG +H+A G V++ +++ +N+ G+ P+H A+ + + +L + I+ T P+H AS G+ +++ LL+ + + G P+H A+ GH I+ +LLQ Sbjct: 634 ITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758 HSP 14 Score: 59.3066 bits (142), Expect = 8.170e-9 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V VA ++ + G+ I+ T P+H AS G ++++T LL+ + +T +G P+H A+ GH+ I+ +LL+ Sbjct: 647 QEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097]) HSP 1 Score: 100.908 bits (250), Expect = 2.319e-22 Identity = 59/209 (28.23%), Postives = 104/209 (49.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++ +LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 188 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 375 HSP 2 Score: 91.2781 bits (225), Expect = 4.018e-19 Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++ +LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 83.5741 bits (205), Expect = 1.331e-16 Identity = 41/149 (27.52%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLL 403 HSP 4 Score: 80.1073 bits (196), Expect = 1.673e-15 Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + L+++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++++ LLQ +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 5 Score: 78.1814 bits (191), Expect = 7.768e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ + T SG P+H +S GH ++ +LLE + +G P+H A+ G ++++KLLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.7962 bits (190), Expect = 8.421e-15 Identity = 46/182 (25.27%), Postives = 86/182 (47.25%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VE+ +I G++++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 7 Score: 76.2554 bits (186), Expect = 2.747e-14 Identity = 45/176 (25.57%), Postives = 84/176 (47.73%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G AD V + LK G+ +H++ G +VA ++++ G++ T G P+H A+ G +E++ LLL++N + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL++ Sbjct: 493 GKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLT 668 HSP 8 Score: 74.3294 bits (181), Expect = 1.189e-13 Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ P+H A+ GH +++ LLL R+ N+ + G+ P+H A+ + + ++L + ID M + L +A + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLL 733 HSP 9 Score: 72.7886 bits (177), Expect = 4.530e-13 Identity = 36/153 (23.53%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ P+H A+ G +++++LLL R+ ++ SG+ P+H A+ + ++L + I+ P+H AS G+++++ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 766 HSP 10 Score: 70.0922 bits (170), Expect = 2.542e-12 Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G+ + TT G P+H +S G ++ ++LLE T G P+H A+ G E++KLLL++N + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 481 DDQT--PLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLL 634 HSP 11 Score: 68.5514 bits (166), Expect = 8.875e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L + N E+ L +A ++ Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLL 469 HSP 12 Score: 67.3958 bits (163), Expect = 1.986e-11 Identity = 64/282 (22.70%), Postives = 114/282 (40.43%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRND------EIAFDKIWKSSDFIRENIY------SGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY-------------PVDIAFQCGHL 951 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L G +H A+ G SE+++ LL+ +E + P+H ++ G I++ LL++ D + L L+ + D +L+ F + + + KI +++N SGL L +A + ++ +LDK T H ++KN+ D I LEY PV +A Q GH+ Sbjct: 383 GFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMD----IATTLLEYGADANAITKQGIAPVHLAAQEGHV 660 HSP 13 Score: 63.5438 bits (153), Expect = 3.495e-10 Identity = 31/125 (24.80%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 + QG +H+A G V++ +++ +N+ G+ P+H A+ + + +L + I+ T P+H AS G+ +++ LL+ + + G P+H A+ GH I+ +LLQ Sbjct: 643 ITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 767 HSP 14 Score: 59.3066 bits (142), Expect = 8.171e-9 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V VA ++ + G+ I+ T P+H AS G ++++T LL+ + +T +G P+H A+ GH+ I+ +LL+ Sbjct: 656 QEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 767
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Match: tpm4 (tropomyosin 4 [Source:Xenbase;Acc:XB-GENE-6077097]) HSP 1 Score: 100.908 bits (250), Expect = 2.328e-22 Identity = 59/209 (28.23%), Postives = 104/209 (49.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++ +LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 179 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 366 HSP 2 Score: 91.2781 bits (225), Expect = 4.032e-19 Identity = 47/166 (28.31%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++ +LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 196 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFTARNDITPLHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 361 HSP 3 Score: 83.5741 bits (205), Expect = 1.335e-16 Identity = 41/149 (27.52%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D GS I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 246 LHVASKRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLL 394 HSP 4 Score: 80.1073 bits (196), Expect = 1.678e-15 Identity = 45/163 (27.61%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + L+++ LLL+ I+ T SG+ PIH A+ GH I+ L + + RG +H A+ G ++++ LLQ +++ K ++ L ++ + + D VQ L+ Sbjct: 331 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLL 493 HSP 5 Score: 78.1814 bits (191), Expect = 7.793e-15 Identity = 41/154 (26.62%), Postives = 75/154 (48.70%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ + T SG P+H +S GH ++ +LLE + +G P+H A+ G ++++KLLLQ+ D + L +A LL+ Sbjct: 439 RGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLL 592 HSP 6 Score: 77.7962 bits (190), Expect = 8.448e-15 Identity = 46/182 (25.27%), Postives = 86/182 (47.25%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VE+ +I G++++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 19 YLRAARSGNLEKALDYLKNGVDINICNQNGLNALHLASKEGHVEIVSELIQRGADVDASTKKGNTALHIASLAGQTEVVRVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 196 HSP 7 Score: 76.2554 bits (186), Expect = 2.755e-14 Identity = 45/176 (25.57%), Postives = 84/176 (47.73%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G AD V + LK G+ +H++ G +VA ++++ G++ T G P+H A+ G +E++ LLL++N + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL++ Sbjct: 484 GKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLT 659 HSP 8 Score: 74.3294 bits (181), Expect = 1.193e-13 Identity = 41/153 (26.80%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ P+H A+ GH +++ LLL R+ N+ + G+ P+H A+ + + ++L + ID M + L +A + V L+ Sbjct: 572 GLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLL 724 HSP 9 Score: 72.7886 bits (177), Expect = 4.543e-13 Identity = 36/153 (23.53%), Postives = 78/153 (50.98%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ P+H A+ G +++++LLL R+ ++ SG+ P+H A+ + ++L + I+ P+H AS G+++++ LLQ +++ K + L A Q + +L+ Sbjct: 605 GYTPLHIAAKKNQMDIATTLLEYGADANAITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLL 757 HSP 10 Score: 70.0922 bits (170), Expect = 2.549e-12 Identity = 42/156 (26.92%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G+ + TT G P+H +S G ++ ++LLE T G P+H A+ G E++KLLL++N + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 472 DDQT--PLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMDIATTLL 625 HSP 11 Score: 68.5514 bits (166), Expect = 8.901e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L + N E+ L +A ++ Q + V+ L+ Sbjct: 251 KRGNANMVKLLLDRGSKIDAKTRDGLTPLHCGARSGHEQVVGMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLL 460 HSP 12 Score: 67.3958 bits (163), Expect = 1.992e-11 Identity = 64/282 (22.70%), Postives = 114/282 (40.43%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRND------EIAFDKIWKSSDFIRENIY------SGLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY-------------PVDIAFQCGHL 951 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L G +H A+ G SE+++ LL+ +E + P+H ++ G I++ LL++ D + L L+ + D +L+ F + + + KI +++N SGL L +A + ++ +LDK T H ++KN+ D I LEY PV +A Q GH+ Sbjct: 374 GFTPLHIACKKNRLKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLNHHGASPNTTNVRGETALHMAARAGQSEVVRFLLQNGAQVEAKAKDDQTPLHISARLGKADIVQQLLKQGAYPDAATTSGYTPLHLSSREGHDDVASVLLEHGASFGIVTKKGFTPLHVAAKYGKIEVVKLLLQKNASPDAAGKSGLTPLHVAAHYDNQKVALLLLDKGASPHGAAKNGYTPLHIAAKKNQMD----IATTLLEYGADANAITKQGIAPVHLAAQEGHV 651 HSP 13 Score: 63.5438 bits (153), Expect = 3.504e-10 Identity = 31/125 (24.80%), Postives = 61/125 (48.80%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 + QG +H+A G V++ +++ +N+ G+ P+H A+ + + +L + I+ T P+H AS G+ +++ LL+ + + G P+H A+ GH I+ +LLQ Sbjct: 634 ITKQGIAPVHLAAQEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758 HSP 14 Score: 59.3066 bits (142), Expect = 8.192e-9 Identity = 32/112 (28.57%), Postives = 58/112 (51.79%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EG+ D V L + + G +H+A V VA ++ + G+ I+ T P+H AS G ++++T LL+ + +T +G P+H A+ GH+ I+ +LL+ Sbjct: 647 QEGHVDMVSLLLTRSANVNVSNKSGLTPLHLAAQEDRVSVAEVLANQGAVIDGTTKMFYTPLHVASHYGNIKMVTFLLQHGSKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 758
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Match: Ank2 (ankyrin 2, brain [Source:MGI Symbol;Acc:MGI:88025]) HSP 1 Score: 105.916 bits (263), Expect = 1.556e-24 Identity = 59/209 (28.23%), Postives = 105/209 (50.24%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + ++ G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ ++KLLL+R I+ + G+ P+HCA+ GH Q+++LLL+RK + + S L +A Q ++C V+ L + D++ D + + LHV A C R+ K +LDK + Sbjct: 207 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVEC------VKHLLQYKAPVDDVTLDYL------------TALHV---AAHCGHYRVTKLLLDKRAN 394 HSP 2 Score: 98.9821 bits (245), Expect = 3.201e-22 Identity = 47/140 (33.57%), Postives = 80/140 (57.14%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G V VA ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH ++++LLLER + G+ P+H A+ G H++ +K LLQ K +D Sbjct: 224 LQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVD 363 HSP 3 Score: 83.1889 bits (204), Expect = 6.885e-17 Identity = 64/254 (25.20%), Postives = 107/254 (42.13%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFI---------NRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 FL+ + GN D V +YLK + G ++H+A G V + + ++ GS+++ T G +H AS G E++ +L++ +I ++ +G P++ A+ H +++K LLE N G P+ A GH Q + +LL+ D K + L +A + LL+ VQ F N N + DF N + LHV N +VK +LD+ Sbjct: 46 SFLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTN---MVKLLLDR 292 HSP 4 Score: 78.5666 bits (192), Expect = 2.340e-15 Identity = 41/153 (26.80%), Postives = 82/153 (53.59%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G + ++++D G I+ +T G+ P+H A+ G +++ LLLER + T +G+ P+H A+ G H E +K LL+ ++ + +H A+ GH ++ KLLL ++ + + + L +A + ++ ++LL+ Sbjct: 270 GITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLV 422 HSP 5 Score: 55.8398 bits (133), Expect = 4.310e-8 Identity = 26/94 (27.66%), Postives = 50/94 (53.19%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECED 480 ++H+A G V ++++D +N G P+H A + ++++ LL++ I+ T SG+ PIH A+ GH I+ LLL+ + + + Sbjct: 372 ALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTN 465
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 103.219 bits (256), Expect = 3.603e-23 Identity = 59/206 (28.64%), Postives = 105/206 (50.97%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK Sbjct: 188 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDK 372 HSP 2 Score: 90.1225 bits (222), Expect = 6.530e-19 Identity = 46/166 (27.71%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R+ I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 82.0333 bits (201), Expect = 2.670e-16 Identity = 40/149 (26.85%), Postives = 85/149 (57.05%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R+ I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLL 403 HSP 4 Score: 78.9518 bits (193), Expect = 2.696e-15 Identity = 48/187 (25.67%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H+A G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 679 HSP 5 Score: 77.411 bits (189), Expect = 9.050e-15 Identity = 40/154 (25.97%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H A+ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.411 bits (189), Expect = 9.381e-15 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 766 HSP 7 Score: 77.0258 bits (188), Expect = 1.307e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 733 HSP 8 Score: 76.6406 bits (187), Expect = 1.535e-14 Identity = 42/163 (25.77%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D ++ + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 9 Score: 71.633 bits (174), Expect = 7.190e-13 Identity = 43/182 (23.63%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 10 Score: 68.5514 bits (166), Expect = 7.165e-12 Identity = 52/210 (24.76%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ + I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 469 HSP 11 Score: 67.781 bits (164), Expect = 1.343e-11 Identity = 31/125 (24.80%), Postives = 63/125 (50.40%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G +H A+ GH I+ +LLQ Sbjct: 643 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQ 767 HSP 12 Score: 65.0846 bits (157), Expect = 9.058e-11 Identity = 36/153 (23.53%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L LA + D L+ Sbjct: 383 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 535 HSP 13 Score: 62.7734 bits (151), Expect = 4.269e-10 Identity = 30/116 (25.86%), Postives = 62/116 (53.45%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G +H A+ GH+ I+ +LL+ N + Sbjct: 656 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771 HSP 14 Score: 58.5362 bits (140), Expect = 1.078e-8 Identity = 33/157 (21.02%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H A+ GH + LL + + L +A + +++ LL+ Sbjct: 412 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 568
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 103.219 bits (256), Expect = 3.616e-23 Identity = 59/206 (28.64%), Postives = 105/206 (50.97%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK Sbjct: 199 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDK 383 HSP 2 Score: 90.1225 bits (222), Expect = 6.552e-19 Identity = 46/166 (27.71%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R+ I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 216 LQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 381 HSP 3 Score: 82.0333 bits (201), Expect = 2.678e-16 Identity = 40/149 (26.85%), Postives = 85/149 (57.05%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R+ I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 266 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLL 414 HSP 4 Score: 78.9518 bits (193), Expect = 2.704e-15 Identity = 48/187 (25.67%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H+A G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 504 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 690 HSP 5 Score: 77.411 bits (189), Expect = 9.079e-15 Identity = 40/154 (25.97%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H A+ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 459 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 612 HSP 6 Score: 77.411 bits (189), Expect = 9.410e-15 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 625 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 777 HSP 7 Score: 77.0258 bits (188), Expect = 1.311e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 592 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 744 HSP 8 Score: 76.6406 bits (187), Expect = 1.540e-14 Identity = 42/163 (25.77%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D ++ + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 351 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 513 HSP 9 Score: 71.633 bits (174), Expect = 7.211e-13 Identity = 43/182 (23.63%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 39 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 216 HSP 10 Score: 68.5514 bits (166), Expect = 7.185e-12 Identity = 52/210 (24.76%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ + I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 271 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 480 HSP 11 Score: 67.781 bits (164), Expect = 1.347e-11 Identity = 31/125 (24.80%), Postives = 63/125 (50.40%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G +H A+ GH I+ +LLQ Sbjct: 654 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQ 778 HSP 12 Score: 65.0846 bits (157), Expect = 9.082e-11 Identity = 36/153 (23.53%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L LA + D L+ Sbjct: 394 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 546 HSP 13 Score: 62.7734 bits (151), Expect = 4.280e-10 Identity = 30/116 (25.86%), Postives = 62/116 (53.45%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G +H A+ GH+ I+ +LL+ N + Sbjct: 667 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 782 HSP 14 Score: 58.5362 bits (140), Expect = 1.081e-8 Identity = 33/157 (21.02%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H A+ GH + LL + + L +A + +++ LL+ Sbjct: 423 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 579
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 103.219 bits (256), Expect = 3.636e-23 Identity = 59/206 (28.64%), Postives = 105/206 (50.97%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK Sbjct: 188 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDK 372 HSP 2 Score: 90.1225 bits (222), Expect = 6.585e-19 Identity = 46/166 (27.71%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R+ I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 82.0333 bits (201), Expect = 2.691e-16 Identity = 40/149 (26.85%), Postives = 85/149 (57.05%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R+ I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLL 403 HSP 4 Score: 78.9518 bits (193), Expect = 2.717e-15 Identity = 48/187 (25.67%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H+A G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 679 HSP 5 Score: 77.411 bits (189), Expect = 9.120e-15 Identity = 40/154 (25.97%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H A+ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.411 bits (189), Expect = 9.453e-15 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 766 HSP 7 Score: 77.0258 bits (188), Expect = 1.317e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 733 HSP 8 Score: 76.6406 bits (187), Expect = 1.547e-14 Identity = 42/163 (25.77%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D ++ + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 9 Score: 71.633 bits (174), Expect = 7.242e-13 Identity = 43/182 (23.63%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 10 Score: 68.5514 bits (166), Expect = 7.215e-12 Identity = 52/210 (24.76%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ + I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 469 HSP 11 Score: 67.781 bits (164), Expect = 1.353e-11 Identity = 31/125 (24.80%), Postives = 63/125 (50.40%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G +H A+ GH I+ +LLQ Sbjct: 643 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQ 767 HSP 12 Score: 65.0846 bits (157), Expect = 9.118e-11 Identity = 36/153 (23.53%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L LA + D L+ Sbjct: 383 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 535 HSP 13 Score: 62.7734 bits (151), Expect = 4.297e-10 Identity = 30/116 (25.86%), Postives = 62/116 (53.45%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G +H A+ GH+ I+ +LL+ N + Sbjct: 656 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771 HSP 14 Score: 58.5362 bits (140), Expect = 1.085e-8 Identity = 33/157 (21.02%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H A+ GH + LL + + L +A + +++ LL+ Sbjct: 412 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 568
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Match: Ank3 (ankyrin 3, epithelial [Source:MGI Symbol;Acc:MGI:88026]) HSP 1 Score: 103.219 bits (256), Expect = 3.677e-23 Identity = 59/206 (28.64%), Postives = 105/206 (50.97%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK Sbjct: 188 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDK 372 HSP 2 Score: 90.1225 bits (222), Expect = 6.655e-19 Identity = 46/166 (27.71%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R+ I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 82.0333 bits (201), Expect = 2.718e-16 Identity = 40/149 (26.85%), Postives = 85/149 (57.05%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R+ I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLL 403 HSP 4 Score: 78.9518 bits (193), Expect = 2.744e-15 Identity = 48/187 (25.67%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H+A G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 679 HSP 5 Score: 77.411 bits (189), Expect = 9.210e-15 Identity = 40/154 (25.97%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H A+ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.411 bits (189), Expect = 9.546e-15 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 766 HSP 7 Score: 77.0258 bits (188), Expect = 1.330e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 733 HSP 8 Score: 76.6406 bits (187), Expect = 1.562e-14 Identity = 42/163 (25.77%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D ++ + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 9 Score: 71.633 bits (174), Expect = 7.309e-13 Identity = 43/182 (23.63%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 10 Score: 68.5514 bits (166), Expect = 7.279e-12 Identity = 52/210 (24.76%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ + I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 469 HSP 11 Score: 67.781 bits (164), Expect = 1.365e-11 Identity = 31/125 (24.80%), Postives = 63/125 (50.40%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G +H A+ GH I+ +LLQ Sbjct: 643 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQ 767 HSP 12 Score: 65.0846 bits (157), Expect = 9.196e-11 Identity = 36/153 (23.53%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L LA + D L+ Sbjct: 383 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 535 HSP 13 Score: 62.7734 bits (151), Expect = 4.332e-10 Identity = 30/116 (25.86%), Postives = 62/116 (53.45%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G +H A+ GH+ I+ +LL+ N + Sbjct: 656 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771 HSP 14 Score: 58.5362 bits (140), Expect = 1.093e-8 Identity = 33/157 (21.02%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H A+ GH + LL + + L +A + +++ LL+ Sbjct: 412 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 568
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Match: sp|G5E8K5|ANK3_MOUSE (Ankyrin-3 OS=Mus musculus OX=10090 GN=Ank3 PE=1 SV=1) HSP 1 Score: 103.219 bits (256), Expect = 2.581e-22 Identity = 59/206 (28.64%), Postives = 105/206 (50.97%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK Sbjct: 188 ALHIAARKDDTKAAALLLQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDK 372 HSP 2 Score: 90.1225 bits (222), Expect = 4.671e-18 Identity = 46/166 (27.71%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R+ I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 205 LQNDTNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 370 HSP 3 Score: 82.0333 bits (201), Expect = 1.907e-15 Identity = 40/149 (26.85%), Postives = 85/149 (57.05%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R+ I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 255 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLL 403 HSP 4 Score: 78.9518 bits (193), Expect = 1.925e-14 Identity = 48/187 (25.67%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H+A G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 493 GKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 679 HSP 5 Score: 77.411 bits (189), Expect = 6.462e-14 Identity = 40/154 (25.97%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H A+ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 448 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 601 HSP 6 Score: 77.411 bits (189), Expect = 6.697e-14 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 614 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLL 766 HSP 7 Score: 77.0258 bits (188), Expect = 9.329e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 581 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 733 HSP 8 Score: 76.6406 bits (187), Expect = 1.096e-13 Identity = 42/163 (25.77%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D ++ + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 340 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 502 HSP 9 Score: 71.633 bits (174), Expect = 5.127e-12 Identity = 43/182 (23.63%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 28 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 205 HSP 10 Score: 68.5514 bits (166), Expect = 5.106e-11 Identity = 52/210 (24.76%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ + I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 260 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRSAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKASPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 469 HSP 11 Score: 67.781 bits (164), Expect = 9.572e-11 Identity = 31/125 (24.80%), Postives = 63/125 (50.40%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G +H A+ GH I+ +LLQ Sbjct: 643 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQ 767 HSP 12 Score: 65.0846 bits (157), Expect = 6.450e-10 Identity = 36/153 (23.53%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L LA + D L+ Sbjct: 383 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLL 535 HSP 13 Score: 62.7734 bits (151), Expect = 3.038e-9 Identity = 30/116 (25.86%), Postives = 62/116 (53.45%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G +H A+ GH+ I+ +LL+ N + Sbjct: 656 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTALHQAAQQGHTHIINVLLQNNAS 771 HSP 14 Score: 58.5362 bits (140), Expect = 7.664e-8 Identity = 33/157 (21.02%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H A+ GH + LL + + L +A + +++ LL+ Sbjct: 412 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLAAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 568
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Match: sp|Q12955|ANK3_HUMAN (Ankyrin-3 OS=Homo sapiens OX=9606 GN=ANK3 PE=1 SV=3) HSP 1 Score: 102.834 bits (255), Expect = 3.791e-22 Identity = 59/209 (28.23%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E+ G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 205 ALHIAARKDDTKAAALLLQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 392 HSP 2 Score: 88.9669 bits (219), Expect = 1.321e-17 Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 222 LQNDNNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 387 HSP 3 Score: 81.6481 bits (200), Expect = 2.736e-15 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 272 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 420 HSP 4 Score: 79.337 bits (194), Expect = 1.856e-14 Identity = 43/163 (26.38%), Postives = 85/163 (52.15%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 357 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 519 HSP 5 Score: 76.6406 bits (187), Expect = 1.262e-13 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 631 GYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 783 HSP 6 Score: 76.6406 bits (187), Expect = 1.273e-13 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 598 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 750 HSP 7 Score: 76.2554 bits (186), Expect = 1.665e-13 Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H ++ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 465 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 618 HSP 8 Score: 76.2554 bits (186), Expect = 1.854e-13 Identity = 45/175 (25.71%), Postives = 82/175 (46.86%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G AD V + L+ G+ +H++ G +VA ++D G+++ T G P+H A+ G LE+ LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+ Sbjct: 510 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLL 684 HSP 9 Score: 72.4034 bits (176), Expect = 2.940e-12 Identity = 44/182 (24.18%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E++K LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 45 YLRAARAGHLEKALDYIKNGVDINICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVKFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 222 HSP 10 Score: 70.8626 bits (172), Expect = 1.002e-11 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 277 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 486 HSP 11 Score: 70.4774 bits (171), Expect = 1.144e-11 Identity = 32/125 (25.60%), Postives = 64/125 (51.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G P+H A+ GH I+ +LLQ Sbjct: 660 VTRQGIASVHLAAQEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 784 HSP 12 Score: 65.855 bits (159), Expect = 3.967e-10 Identity = 31/116 (26.72%), Postives = 63/116 (54.31%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G P+H A+ GH+ I+ +LL+ N + Sbjct: 673 QEGHVDMVSLLLGRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 788 HSP 13 Score: 65.4698 bits (158), Expect = 5.497e-10 Identity = 38/156 (24.36%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G ++ + ++ G++ TT G P+H ++ G ++ LL+ + T G P+H A+ G E+ LLL+++ + + G+ P+H A+ + ++ LLL + + L +A + QMD L+ Sbjct: 498 DDQT--PLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATTLL 651 HSP 14 Score: 64.3142 bits (155), Expect = 1.246e-9 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L L+ + D L+ Sbjct: 400 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLL 552 HSP 15 Score: 57.3806 bits (137), Expect = 1.854e-7 Identity = 32/157 (20.38%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H ++ GH + LL + + L +A + +++ LL+ Sbjct: 429 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVANLLL 585
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Match: sp|Q54KA7|SECG_DICDI (Ankyrin repeat, PH and SEC7 domain containing protein secG OS=Dictyostelium discoideum OX=44689 GN=secG PE=2 SV=1) HSP 1 Score: 97.8265 bits (242), Expect = 1.129e-20 Identity = 51/179 (28.49%), Postives = 90/179 (50.28%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIW 711 I D++G +H A G A++++D G+ I + G P+H A+ G + L L+ ++E + + G P+H A+ GHS+ ++LL++ N+ D P+H AS+ G + +L+Q K RID KN ++ L AI+ D ++LI D ++ + F K + Sbjct: 363 IKDEEGATPLHKASFNGHSSCAKLLVDKGAPICIVDSQGATPLHKAAFNGRSKCLATLIRSGAELEVKDSQGGTPLHNAAYNGHSDCCRILLKKGANVNAVDTHSSTPLHLASAAGARDTVDVLIQFKARIDAKNFAGKTPLVYAIKKNHSDVARVLIRAGADLDQVSLRSSVDFTKTF 541 HSP 2 Score: 77.411 bits (189), Expect = 5.661e-14 Identity = 44/166 (26.51%), Postives = 80/166 (48.19%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFIN 678 D + S+H+A G +++ ++I + I + G P+H AS G LL+++ I + G P+H A+ G S+ L L+ +E +D++G P+H A+ GH ++LL++ ++ + S + L LA + D V +LI + D N Sbjct: 332 DSRQSTSLHLAAFNGLLDMVDLLIRYKAQINIKDEEGATPLHKASFNGHSSCAKLLVDKGAPICIVDSQGATPLHKAAFNGRSKCLATLIRSGAELEVKDSQGGTPLHNAAYNGHSDCCRILLKKGANVNAVDTHSSTPLHLASAAGARDTVDVLIQFKARIDAKN 497 HSP 3 Score: 75.8702 bits (185), Expect = 1.737e-13 Identity = 44/159 (27.67%), Postives = 82/159 (51.57%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGS-NIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 I D G+ + A G +E +++++ G ++ + P+H AS E + LL D T +G P+H+AS+GG+ + ++LL++ + + DN + P+H AS GH + LLL++ ++D ++ S L A + +DCV+ L+ Sbjct: 97 IKDSAGNTPLQWASSRGHLECIKLLVEKGGVDVNTKDDKNGTPLHKASLFASAECVLYLLNGKADPRAVTTNGETPLHHASAGGNPQCVELLIKADSKVNAVDNDCITPLHQASFSGHSSCVSLLLKKGAKVDPRDIHGISPLHNAASAGYVDCVEQLV 255 HSP 4 Score: 71.633 bits (174), Expect = 4.336e-12 Identity = 43/159 (27.04%), Postives = 77/159 (48.43%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSN-IECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +VD+ G +H A G EV ++ I+C + +H A+ G L+++ LL+ I + G P+H AS GHS KLL+++ I D++G P+H A+ G + L L++ ++ K++ + L A + DC ++L+ Sbjct: 296 MVDEMGETPLHKAAFNGHKEVCEYLLYLDPTMIDCRDSRQSTSLHLAAFNGLLDMVDLLIRYKAQINIKDEEGATPLHKASFNGHSSCAKLLVDKGAPICIVDSQGATPLHKAAFNGRSKCLATLIRSGAELEVKDSQGGTPLHNAAYNGHSDCCRILL 454 HSP 5 Score: 70.0922 bits (170), Expect = 1.456e-11 Identity = 36/131 (27.48%), Postives = 70/131 (53.44%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDC 573 DD+ +H A + E +++ ++ TT G P+H+AS+ G + + LL++ + + N + P+H AS GHS + LLL++ ++ D G+ P+H A+S G++ ++ L++ I+C Sbjct: 133 DDKNGTPLHKASLFASAECVLYLLNGKADPRAVTTNGETPLHHASAGGNPQCVELLIKADSKVNAVDNDCITPLHQASFSGHSSCVSLLLKKGAKVDPRDIHGISPLHNAASAGYVDCVEQLVRNGENINC 263 HSP 6 Score: 68.9366 bits (167), Expect = 3.478e-11 Identity = 40/144 (27.78%), Postives = 71/144 (49.31%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELA 609 V G +H A GG + ++I S + + P+H AS G ++LLL++ ++ G+ P+H A+S G+ + ++ L+ NI C D G+ P+H G+LQ+ K L++ +I+ + M E+ L A Sbjct: 165 VTTNGETPLHHASAGGNPQCVELLIKADSKVNAVDNDCITPLHQASFSGHSSCVSLLLKKGAKVDPRDIHGISPLHNAASAGYVDCVEQLVRNGENINCVDIEGVTPLHHTCFNGNLQLTKRLIELGAKINMVDEMGETPLHKA 308 HSP 7 Score: 57.7658 bits (138), Expect = 1.006e-7 Identity = 38/152 (25.00%), Postives = 74/152 (48.68%), Query Frame = 1 Query: 199 SIHIACMGGFVE-VARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLER-NCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 S+H G +E ++ ++ + ++ + + P+H+A+ G + LL++ + + ++G P+ +ASS GH E +KLL+E+ ++ +D++ P+H AS + + LL K E+ L A CV+LLI Sbjct: 38 SVHYYSSTGDIEKLSNLLNNSATSPDTPDSEKRTPLHHAAFCGSAACVNFLLDKKANANIKDSAGNTPLQWASSRGHLECIKLLVEKGGVDVNTKDDKNGTPLHKASLFASAECVLYLLNGKADPRAVTTNGETPLHHASAGGNPQCVELLI 189 HSP 8 Score: 56.9954 bits (136), Expect = 1.779e-7 Identity = 38/122 (31.15%), Postives = 67/122 (54.92%), Query Frame = 1 Query: 295 RP-IHYASSRGCLEILTLLLERNCDIECETNSGMR-PIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQR-KCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 RP +HY SS G +E L+ LL + +S R P+H+A+ G + + LL++ N +D+ G P+ ASS GHL+ +KLL+++ ++ K++ + + L A +CV L++ Sbjct: 36 RPSVHYYSSTGDIEKLSNLLNNSATSPDTPDSEKRTPLHHAAFCGSAACVNFLLDKKANANIKDSAGNTPLQWASSRGHLECIKLLVEKGGVDVNTKDDKNGTPLHKASLFASAECVLYLLN 157
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Match: sp|O70511|ANK3_RAT (Ankyrin-3 OS=Rattus norvegicus OX=10116 GN=Ank3 PE=1 SV=3) HSP 1 Score: 96.6709 bits (239), Expect = 3.306e-20 Identity = 60/217 (27.65%), Postives = 106/217 (48.85%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECE--------TTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ +N + E T G P+H A+ G + + TLLL R ++ + + P+H AS G++ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + D + ++D++ + LHV A C ++ K +LDK + Sbjct: 205 ALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHNVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVLLDKKAN 400 HSP 2 Score: 88.9669 bits (219), Expect = 1.321e-17 Identity = 44/155 (28.39%), Postives = 82/155 (52.90%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + G +HIA G + VA ++++ + ++ + P+H AS RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++L+ Sbjct: 241 ESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLL 395 HSP 3 Score: 81.6481 bits (200), Expect = 3.464e-15 Identity = 40/149 (26.85%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ N ++ N + +H A+ GH ++ K+LL +K + K + L +A + ++ ++LL+ Sbjct: 280 LHVASKRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLL 428 HSP 4 Score: 78.9518 bits (193), Expect = 2.349e-14 Identity = 43/163 (26.38%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 169 NTIVDDQGH---YSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N VDD + ++H+A G +VA++++D +N + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G ++++ L+Q +++ K ++ L ++ + + D VQ L+ Sbjct: 365 NVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLL 527 HSP 5 Score: 77.7962 bits (190), Expect = 4.813e-14 Identity = 47/187 (25.13%), Postives = 88/187 (47.06%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G AD V + L+ G+ +H++ G +VA ++D G+++ T G P+H A+ G LE+ +LLL+++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ + I LL+ + + + LA Q +D V LL+S + N++ Sbjct: 518 GKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKS 704 HSP 6 Score: 77.0258 bits (188), Expect = 9.677e-14 Identity = 39/153 (25.49%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T LLE D T G+ +H A+ GH +++ LLL RN N+ + G+ P+H A+ + + ++L+ + +D + M + L + + V L+ Sbjct: 606 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLL 758 HSP 7 Score: 77.0258 bits (188), Expect = 1.087e-13 Identity = 35/153 (22.88%), Postives = 79/153 (51.63%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G++ T G+ +H A+ G +++++LLL RN ++ SG+ P+H A+ + ++L+ + +++ + G P+H G+++I+ LLQ +++ K + L A Q + +L+ Sbjct: 639 GYTPLHIAAKKNQMDIATSLLEYGADANAVTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLL 791 HSP 8 Score: 76.2554 bits (186), Expect = 1.612e-13 Identity = 39/154 (25.32%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV R ++ G+ +E + P+H ++ G +I+ LL++ T SG P+H ++ GH ++ LL+ ++ +G P+H A+ G L++ LLLQ+ D + L +A LL+ Sbjct: 473 RGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLLQKSASPDAAGKSGLTPLHVAAHYDNQKVALLLL 626 HSP 9 Score: 72.4034 bits (176), Expect = 3.198e-12 Identity = 59/261 (22.61%), Postives = 109/261 (41.76%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDF-----INRNDEIAFDKIWKSS------------------DFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 +L+ + G+ + Y+K+ I + G ++H+A G VEV ++ +N++ T G +H AS G E++ +L+ ++ ++ +G P++ A+ H E+++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ D +NR E F + ++ DF N + LHV N +VK +LD+ Sbjct: 45 YLRAARAGHLEKALDYIKNGVDVNICNQNGLNALHLASKEGHVEVVSELLQREANVDAATKKGNTALHIASLAGQAEVVKVLVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLDNGASQSLATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDTNADIESKMVVNRATESGFTPLHIAAHYGNINVATLLLNRAAAVDFTARNDITPLHVASKRGNAN---MVKLLLDR 298 HSP 10 Score: 70.8626 bits (172), Expect = 7.919e-12 Identity = 32/125 (25.60%), Postives = 64/125 (51.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 V QG S+H+A G V++ +++ +N+ G+ P+H A+ + + +L+ + ++ +T G P+H G+ +I+ LL+ + + + G P+H A+ GH I+ +LLQ Sbjct: 668 VTRQGIASVHLAAQEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQ 792 HSP 11 Score: 70.8626 bits (172), Expect = 9.627e-12 Identity = 53/210 (25.24%), Postives = 89/210 (42.38%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 K GNA+ V L G +H G +V +++D + I +T G+ P+H A+ L + LLL+ N ++ TN + +H A+ GH ++ K+LL++ N I C+ NR G+ PIH A+ GH+ I+ L+ + N E+ L +A +S Q + V+ L+ Sbjct: 285 KRGNANMVKLLLDRGAKIDAKTRDGLTPLHCGARSGHEQVVEMLLDRAAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHNVPVDDVTNDYLTALHVAAHCGHYKVAKVLLDKKANPNAKALNGFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLV 494 HSP 12 Score: 66.2402 bits (160), Expect = 2.547e-10 Identity = 31/116 (26.72%), Postives = 63/116 (54.31%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+ D V L + + + G +H+A V VA ++++ G++++ +T G P+H G ++I+ LL+ + + +T +G P+H A+ GH+ I+ +LL+ N + Sbjct: 681 QEGHVDMVSLLLSRNANVNLSNKSGLTPLHLAAQEDRVNVAEVLVNQGAHVDAQTKMGYTPLHVGCHYGNIKIVNFLLQHSAKVNAKTKNGYTPLHQAAQQGHTHIINVLLQNNAS 796 HSP 13 Score: 63.929 bits (154), Expect = 1.579e-9 Identity = 35/153 (22.88%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++I+ T G+ PIH A+ G + I++ L+ G +H A+ G +E+++ L++ +E + P+H ++ G I++ LLQ+ + + L L+ + D L+ Sbjct: 408 GFTPLHIACKKNRIRVMELLLKHGASIQAVTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLL 560 HSP 14 Score: 57.3806 bits (137), Expect = 1.858e-7 Identity = 32/157 (20.38%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G V + ++ G++ G +H A+ G E++ L++ +E + P+H ++ G ++I++ LL++ + G P+H ++ GH + LL + + L +A + +++ LL+ Sbjct: 437 VTESGLTPIHVAAFMGHVNIVSQLMHHGASPNTTNVRGETALHMAARSGQAEVVRYLVQDGAQVEAKAKDDQTPLHISARLGKADIVQQLLQQGASPNAATTSGYTPLHLSAREGHEDVAAFLLDHGASLSITTKKGFTPLHVAAKYGKLEVASLLL 593
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Match: sp|Q8C8R3|ANK2_MOUSE (Ankyrin-2 OS=Mus musculus OX=10090 GN=Ank2 PE=1 SV=2) HSP 1 Score: 96.2857 bits (238), Expect = 4.480e-20 Identity = 60/214 (28.04%), Postives = 105/214 (49.07%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECE--------TTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ N + + T G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ ++KLLL+R I+ + G+ P+HCA+ GH Q+++LLL+RK + + S L +A Q ++CV+ L+ + D++ D + + LHV A C R+ K +LDK Sbjct: 195 ALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPV------DDVTLDYL------------TALHV---AAHCGHYRVTKLLLDK 387 HSP 2 Score: 95.9005 bits (237), Expect = 7.341e-20 Identity = 49/148 (33.11%), Postives = 83/148 (56.08%), Query Frame = 1 Query: 127 NADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 NAD ++S + + G +HIA G V VA ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH ++++LLLER + G+ P+H A+ G H++ +K LLQ K +D Sbjct: 217 NAD-----VQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVD 359 HSP 3 Score: 79.7221 bits (195), Expect = 1.252e-14 Identity = 58/229 (25.33%), Postives = 95/229 (41.48%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDE 690 +H+A G + ++++D G I+ +T G+ P+H A+ G +++ LLLER + T +G+ P+H A+ G GH + KLLL++ N I C+ NR G+ PIH A+ GHL I+ LLLQ D N E+ L +A ++ Q++ V+ L+ L D R ++ Sbjct: 270 LHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERKAPLLARTKNGLSPLHMAAQGDHVECVKHLLQYKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQ 498 HSP 4 Score: 79.337 bits (194), Expect = 1.539e-14 Identity = 40/150 (26.67%), Postives = 81/150 (54.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V ++++D +N G P+H A + ++++ LL++ I+ T SG+ PIH A+ GH I+ LLL+ + + + RG +H A+ G +++++ LL+ +D + ++ L +A + + + VQLL+ Sbjct: 368 ALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLL 517 HSP 5 Score: 78.5666 bits (192), Expect = 3.359e-14 Identity = 54/218 (24.77%), Postives = 98/218 (44.95%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD-----FINRNDEIAFDKIWKSSDFIRENI 741 FL+ + GN D V +YLK + G ++H+A G V + + ++ GS+++ T G +H AS G E++ +L++ +I ++ +G P++ A+ H +++K LLE N G P+ A GH Q + +LL+ D K + L +A + LL+ D +NR E F + ++ + N+ Sbjct: 35 FLRAARAGNLDKVVEYLKGGIDINTCNQNGLNALHLAAKEGHVGLVQELLGRGSSVDSATKKGNTALHIASLAGQAEVVKVLVKEGANINAQSQNGFTPLYMAAQENHIDVVKYLLENGANQSTATEDGFTPLAVALQQGHNQAVAILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNV 248 HSP 6 Score: 77.411 bits (189), Expect = 7.455e-14 Identity = 43/153 (28.10%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HI+ G V+VA ++++ G+ T G P+H A+ G L++ LLL+R + +G+ P+H A+ + ++ LLLE+ + G P+H A+ +QI LL + + L LA Q D V LL+ Sbjct: 530 GYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLL 682 HSP 7 Score: 76.6406 bits (187), Expect = 1.177e-13 Identity = 44/158 (27.85%), Postives = 74/158 (46.84%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + + +G ++H+A G VEV R ++ G+ ++ P+H AS G EI+ LLL+ + T +G P+H ++ G ++ +LLE +G P+H A+ G L + KLLLQR+ D + L +A LL+ Sbjct: 459 VTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLL 616 HSP 8 Score: 76.2554 bits (186), Expect = 1.807e-13 Identity = 42/153 (27.45%), Postives = 74/153 (48.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G L I+ LLL+ + G +H A+ G E+++ LL ++ P+H AS G +I++LLLQ D + L ++ + Q+D +L+ Sbjct: 398 GFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLL 550 HSP 9 Score: 69.3218 bits (168), Expect = 3.320e-11 Identity = 41/176 (23.30%), Postives = 79/176 (44.89%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 +EG D L++ +++ +G +H+A G ++VA++++ + + G+ P+H A+ ++ LLLE+ +G P+H A+ +I LL +G+ P+H AS GH ++ LLL + I + L LA Q +++ +L Sbjct: 539 REGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADIL 714 HSP 10 Score: 68.5514 bits (166), Expect = 5.902e-11 Identity = 36/144 (25.00%), Postives = 69/144 (47.92%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELA 609 V QG +H+A G ++ +++D G+NI T G+ +H A+ + + +L + D + T G P+ A G+ +++ LL++ N+ + G P+H A+ GH I+ +LLQ + + + L +A Sbjct: 658 VTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLLKQGANVNAKTKNGYTPLHQAAQQGHTHIINVLLQHGAKPNATTANGNTALAIA 801 HSP 11 Score: 66.2402 bits (160), Expect = 2.627e-10 Identity = 37/150 (24.67%), Postives = 73/150 (48.67%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +HIA G E+ ++++ ++ + TT G P+H ++ G +++ ++LLE T G P+H A+ G ++ KLLL+R + G+ P+H A+ + ++ LLL++ + L +A + QM L++ Sbjct: 501 LHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKNGLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLN 650 HSP 12 Score: 62.003 bits (149), Expect = 5.662e-9 Identity = 36/169 (21.30%), Postives = 76/169 (44.97%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 + + G IH+A G + + +++ G++ + G +H A+ G +E++ LL ++ P+H AS G +EI++LLL+ + + G P+H ++ G + + +LL+ + L +A + +D +LL+ + D +N Sbjct: 427 ITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARAREEQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQVDVASVLLEAGAAHSLATKKGFTPLHVAAKYGSLDVAKLLLQRRAAADSAGKN 595 HSP 13 Score: 62.003 bits (149), Expect = 6.928e-9 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA ++++ G++ G P+H A+ + ++I + LL + T G+ P+H AS GH++++ LLL++ NI G+ +H A+ + + +L + D + + L +A + V L+ Sbjct: 596 GLTPLHVAAHYDNQKVALLLLEKGASPHATAKNGYTPLHIAAKKNQMQIASTLLNYGAETNTVTKQGVTPLHLASQEGHTDMVTLLLDKGANIHMSTKSGLTSLHLAAQEDKVNVADILTKHGADRDAYTKLGYTPLIVACHYGNVKMVNFLL 748
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Match: J9HSB7 (AAEL017480-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AaeL_AAEL017480 PE=4 SV=1) HSP 1 Score: 131.724 bits (330), Expect = 2.467e-31 Identity = 57/155 (36.77%), Postives = 94/155 (60.65%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV + +ID G+N++ G P+HYAS G LE++ L++ +++ N G P+HYAS GH E++KLL++ N++ DN G P+H AS GHL+++KLL+ +D KN + L +A Q+ +++ V+LLI Sbjct: 139 NEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLI 293 HSP 2 Score: 122.865 bits (307), Expect = 3.798e-28 Identity = 75/286 (26.22%), Postives = 134/286 (46.85%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD-----------FINRNDEIAFDKIW----KSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVC 996 ++G +H A + G +EV +++ID G+N++ G P+H+AS G LE++ LL++ +++ N P+HYAS G E++K L++ N++ DN G P+H AS GHL+++KLL+ +D N + L A ++ +++ V+ +I D + +RN + K + D + ++ LH A +VK + +DDE DT D++ D L G + V + G + T + R +S H+ Sbjct: 7 NEGWTPLHYASLNGHLEVVKLLIDNGANVDTTQNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLH---YASRNGHLEVVKLL----IDDEANVDTTDNEGWTPLHDASLIGHLEV-----VKLLIDNGANVDTKNTRRPTSLHIA 280 HSP 3 Score: 121.709 bits (304), Expect = 9.989e-28 Identity = 52/157 (33.12%), Postives = 90/157 (57.32%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D++G +H A G +EV +++ID G+N++ G P+HYAS G LE++ +++ +++ N G P+HYAS G E++K L++ N++ N G P+H AS GHL+++KLL+ + +D +N + L A ++ V+LLI Sbjct: 104 TDNEGWTPLHYASRNGHLEVVKLLIDNGANVDTTRNEGWTPLHYASRNGRLEVVKFMIDNGANVDTTDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLI 260 HSP 4 Score: 118.627 bits (296), Expect = 1.413e-26 Identity = 53/157 (33.76%), Postives = 92/157 (58.60%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D++G +H A G +EV + +ID G+N++ G P+HYAS G LE++ LL++ +++ N G P+H AS GH E++KLL++ N++ ++ R +H AS G L+++KLL+ +D KN + L +A ++ ++ V+LLI Sbjct: 170 TDNEGWTPLHYASRNGRLEVVKFLIDNGANVDTTQNEGWTPLHYASRNGHLEVVKLLIDDEANVDTTDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLIDNGANVDTKNTRGSTSLHIASRNGHLEVVKLLI 326 HSP 5 Score: 99.3673 bits (246), Expect = 1.251e-19 Identity = 42/128 (32.81%), Postives = 73/128 (57.03%), Query Frame = 1 Query: 265 NIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 N++ G P+HYAS G LE++ LL++ +++ N G P+H+AS GH E++KLL++ N++ N P+H AS G L+++K L+ +D +N + L A ++ ++ V+LLI Sbjct: 1 NVDTTRNEGWTPLHYASLNGHLEVVKLLIDNGANVDTTQNKGWTPLHFASQNGHLEVVKLLIDNRANVDTTQNEEWTPLHYASRNGRLEVVKFLIDNGANVDTTDNEGWTPLHYASRNGHLEVVKLLI 128 HSP 6 Score: 71.633 bits (174), Expect = 5.061e-10 Identity = 31/98 (31.63%), Postives = 59/98 (60.20%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIE 471 D++G +H A + G +EV +++ID G+N++ + T +H AS G LE++ LL++ +++ + G +H AS GH E++KLL++ N++ Sbjct: 236 TDNEGWTPLHDASLIGHLEVVKLLIDNGANVDTKNTRRPTSLHIASQNGRLEVVKLLIDNGANVDTKNTRGSTSLHIASRNGHLEVVKLLIDNGANVD 333
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Match: Q16FI9 (AAEL014742-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014742 PE=4 SV=1) HSP 1 Score: 132.88 bits (333), Expect = 5.217e-30 Identity = 54/157 (34.39%), Postives = 97/157 (61.78%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D++G +H+A G +EV +++I+ +N++ G+ P+H+AS G LE++ LL++ +++ N G P+H AS GH E++KLL+E N++ N+G+ P+H AS GHL+++KLL+ + +D N + L +A Q+ ++ V+LLI Sbjct: 587 TDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLI 743 HSP 2 Score: 132.109 bits (331), Expect = 8.762e-30 Identity = 65/203 (32.02%), Postives = 112/203 (55.17%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINR-NDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNAD 789 D+G +H+A G +EV +++ID +N++ + G P+H AS G LE++ LL+E +++ + N G P+H+AS GH E++K L++ N++ + G P+H AS GHL+++KLL++ + +D K N + L A Q+ ++ V+ LI NR N + D+ W EN + L V+ + IE A+ Sbjct: 83 DEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLID--------NRANVDTTQDEGWTPLHLAAENGH--LEVVKLLIENRAN 275 HSP 3 Score: 130.183 bits (326), Expect = 4.817e-29 Identity = 53/155 (34.19%), Postives = 95/155 (61.29%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D+G +H+A G++EV +++ID G+N++ G P+H A+ G LE++ LL++ +++ + N G P+H AS GH E++KLL+E N++ + N G P+H AS GHL+++K L+ + +D + + L +A Q+ ++ V+LLI Sbjct: 50 DEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLI 204 HSP 4 Score: 128.257 bits (321), Expect = 1.830e-28 Identity = 53/155 (34.19%), Postives = 94/155 (60.65%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +++A G +EV +++ID +N++ G P+H AS G LE++ LL+E +++ N G+ P+H+AS GH E++KLL++ N++ N G P+H AS GHL+++KLL++ + +D N + L A Q+ ++ V+LLI Sbjct: 556 NKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLI 710 HSP 5 Score: 127.872 bits (320), Expect = 2.591e-28 Identity = 53/155 (34.19%), Postives = 95/155 (61.29%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H+A G +EV +++ID +N++ G+ P++ AS G LE++ LL++ +++ N G P+H AS GH E++KLL+E N++ N+G+ P+H AS GHL+++KLL+ + +D N + L +A Q+ ++ V+LLI Sbjct: 523 NEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLI 677 HSP 6 Score: 127.487 bits (319), Expect = 3.772e-28 Identity = 51/156 (32.69%), Postives = 95/156 (60.90%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++ G +H+A G ++V +++ID G+N++ E G P+H A+ G LE++ LL++ +++ + G P+H A+ GH E++KLL++ N++ + N G P+H AS GHL+++KLL++ + +D K N + L A Q+ ++ V+ LI Sbjct: 16 NNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLI 171 HSP 7 Score: 126.331 bits (316), Expect = 9.028e-28 Identity = 52/155 (33.55%), Postives = 94/155 (60.65%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +++A G +EV +++ID +N++ G P+H AS G LE++ LL++ +++ N G+ P++ AS GH E++KLL++ N++ DN G P+H AS GHL+++KLL++ + +D N + L A Q+ ++ V+LLI Sbjct: 490 NEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLI 644 HSP 8 Score: 125.561 bits (314), Expect = 1.615e-27 Identity = 51/155 (32.90%), Postives = 92/155 (59.35%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV + +ID +N++ G P+H AS G LE++ LL+E +++ + N G P+H+AS GH E++K L++ N++ + G P+H A+ GHL+++KLL++ + +D K N + L +A Q+ ++ V+ LI Sbjct: 149 NEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLI 303 HSP 9 Score: 124.79 bits (312), Expect = 2.914e-27 Identity = 53/155 (34.19%), Postives = 92/155 (59.35%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV +++ID +N++ G P+H AS G LE++ LL+E +++ N G+ P+H+AS GH E++KLL++ N++ N G P+H AS GHL+++KLL+ + +D N + L +A + ++ V+LLI Sbjct: 622 NKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLI 776 HSP 10 Score: 122.094 bits (305), Expect = 2.285e-26 Identity = 52/155 (33.55%), Postives = 88/155 (56.77%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV + +ID +N++ G P+H A+ G LE++ LL+E +++ + N G P+H AS GH E++K L++ N++ G P+H AS GHL+++KLL+ K +D N + L A Q+ ++ V+LLI Sbjct: 215 NEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLI 369 HSP 11 Score: 121.709 bits (304), Expect = 3.229e-26 Identity = 54/166 (32.53%), Postives = 95/166 (57.23%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLL-----------ERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +G +H+A G +EV +++ID +N++ G+ P+H+AS G LE++ LL+ E +++ N G+ P+H+AS GH E++KLL+E N+ N G P+H AS GHL+++KLL++ + +D N + L +A + ++ V+LLI+ Sbjct: 315 EGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLIN 480 HSP 12 Score: 121.709 bits (304), Expect = 3.290e-26 Identity = 50/155 (32.26%), Postives = 96/155 (61.94%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +++A + G +EV +++I+ +N++ G P++ AS G LE++ LL++ +++ N G P+H AS GH E++KLL++ N++ N+G+ P++ AS GHL+++KLL+ K +D +N + L +A Q+ ++ V+LLI Sbjct: 457 NEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLIDNKANVDTTDNEGWTPLHVASQNGHLEVVKLLI 611 HSP 13 Score: 121.709 bits (304), Expect = 3.613e-26 Identity = 49/155 (31.61%), Postives = 92/155 (59.35%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D+G +H+A G +EV +++I+ +N++ + G P+H+AS G LE++ L++ +++ + G P+H A+ GH E++KLL+E N++ + N G P+H AS GHL+++K L+ + +D + L +A Q+ ++ V+LLI Sbjct: 182 DEGWTPLHVASQNGHLEVVKLLIENRANVDTKKNEGWTPLHFASQNGHLEVVKFLIDNRANVDTTQDEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLI 336 HSP 14 Score: 119.783 bits (299), Expect = 1.495e-25 Identity = 52/155 (33.55%), Postives = 90/155 (58.06%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV +++I+ +N+ G P+H+AS G LE++ LL+E +++ N G P++ AS GH E++KLL+ N++ N G P++ AS GHL+++KLL+ K +D N + L +A Q+ ++ V+LLI Sbjct: 391 NKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLI 545 HSP 15 Score: 117.857 bits (294), Expect = 7.367e-25 Identity = 55/166 (33.13%), Postives = 95/166 (57.23%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNI---------ECETTW--GMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV +++ID +N+ +TT G+ P+H+AS G LE++ LL+E ++ N G P+H+AS GH E++KLL+E N++ N G P++ AS GHL+++KLL+ + +D N + L +A ++ ++ V+LLI Sbjct: 347 NKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIENRANVGTTQNEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLI 512 HSP 16 Score: 116.316 bits (290), Expect = 2.428e-24 Identity = 51/166 (30.72%), Postives = 93/166 (56.02%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQ-----------ILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 D+G +H+A G +EV +++I+ +N++ + G P+H AS G LE++ L++ +++ G P+H AS GH E++KLL++ N++ N+G+ P+H AS GHL+ ++KLL++ + +D N + L A Q+ ++ V+LLI Sbjct: 248 DEGWTPLHLAAENGHLEVVKLLIENRANVDTKKNGGWTPLHVASQNGHLEVVKFLIDNRANVDTTQYEGWTPLHVASQNGHLEVVKLLIDNKANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLI 413 HSP 17 Score: 114.775 bits (286), Expect = 7.213e-24 Identity = 44/133 (33.08%), Postives = 83/133 (62.41%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNN 582 ++G +H+A G +EV +++I+ +N++ G+ P+H+AS G LE++ LL++ +++ N G P+H AS GH E++KLL++ N++ N+G+ P++ AS GHL+++KLL+ + +D N Sbjct: 655 NEGWTPLHVASQNGHLEVVKLLIENRANVDTTQNKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTTQN 787 HSP 18 Score: 114.775 bits (286), Expect = 7.486e-24 Identity = 49/155 (31.61%), Postives = 90/155 (58.06%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++G +H A G +EV +++I+ +N++ G P++ AS G LE++ LL+ +++ N G P++ AS GH E++KLL++ N++ N G P+H AS GHL+++KLL+ + +D N + L +A ++ ++ V+LLI Sbjct: 424 NEGWTPLHFASRNGHLEVVKLLIENRANVDTTQNEGWTPLYVASINGHLEVVKLLINNRANVDTTQNEGWTPLYVASKNGHLEVVKLLIDNKANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTKNKGITPLYVASKNGHLEVVKLLI 578 HSP 19 Score: 107.071 bits (266), Expect = 2.732e-21 Identity = 44/138 (31.88%), Postives = 83/138 (60.14%), Query Frame = 1 Query: 235 VARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + + +ID +NI+ G P+H AS G L+++ LL++ +++ E + G P+H A+ G+ E++KLL++ N++ + G P+H A+ GHL+++KLL+ + +D K N + L +A Q+ ++ V+LLI Sbjct: 1 MVKFLIDHNANIDTANNGGRTPLHVASQNGHLKVVKLLIDNGANVDTEGDEGWTPLHLAAENGYLEVVKLLIDNGANVDTTQDEGWTPLHLAAENGHLEVVKLLIDNRANVDTKKNGGWTPLHVASQNGHLEVVKLLI 138 HSP 20 Score: 80.1073 bits (196), Expect = 2.077e-12 Identity = 33/102 (32.35%), Postives = 60/102 (58.82%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRG 489 ++G +H A G +EV +++ID +N++ G P+H AS G LE++ LL++ +++ N G+ P++ AS GH E++KLL++ N++ N G Sbjct: 688 NKGITPLHFASQNGHLEVVKLLIDNRANVDTTQNEGWTPLHVASQNGHLEVVKLLIDNRANVDTTQNKGITPLYVASINGHLEVVKLLIDNRANVDTTQNEG 789
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Match: W4Z5P4 (ANK_REP_REGION domain-containing protein OS=Strongylocentrotus purpuratus OX=7668 PE=4 SV=1) HSP 1 Score: 121.324 bits (303), Expect = 7.321e-29 Identity = 60/159 (37.74%), Postives = 88/159 (55.35%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELF 666 G IH A GG +EV + ++ G+ +E G PIH AS G L+++ L+ R +E N G PIH ASSGGH +++ L+ R +E N G PIHCASSGGHL +++ L+ R R++ N ++ L A ++ +D VQ L+S F Sbjct: 45 GQTPIHCASYGGHLEVVQYLVRRGARVEIGGIDGQAPIHCASRNGHLQVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVSRGARVEIGGNDGQTPLHCASRNGHLDVVQYLVSRGAHF 203 HSP 2 Score: 103.605 bits (257), Expect = 2.784e-22 Identity = 50/150 (33.33%), Postives = 82/150 (54.67%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +++A + G ++V + ++ G+ +E G PIH AS G LE++ L+ R +E G PIH AS GH ++++ L+ R +E N G PIHCASSGGHL +++ L+ R R++ N ++ + A + VQ L+S Sbjct: 16 LNMAALNGHLDVVQYLVSRGAQVEKGGNDGQTPIHCASYGGHLEVVQYLVRRGARVEIGGIDGQAPIHCASRNGHLQVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVS 165 HSP 3 Score: 87.8113 bits (216), Expect = 1.892e-16 Identity = 41/118 (34.75%), Postives = 65/118 (55.08%), Query Frame = 1 Query: 298 PIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 P++ A+ G L+++ L+ R +E N G PIH AS GGH E+++ L+ R +E G PIHCAS GHLQ+++ L+ R R++ N ++ + A + VQ L+S Sbjct: 15 PLNMAALNGHLDVVQYLVSRGAQVEKGGNDGQTPIHCASYGGHLEVVQYLVRRGARVEIGGIDGQAPIHCASRNGHLQVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVS 132 HSP 4 Score: 63.929 bits (154), Expect = 4.503e-8 Identity = 36/111 (32.43%), Postives = 55/111 (49.55%), Query Frame = 1 Query: 139 VWKYLKSDFLNT-IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNI 468 V +YL S I + G IH A GG + V + ++ G+ +E G PIH ASS G L ++ L+ R +E N G P+H AS GH ++++ L+ R + Sbjct: 93 VVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVSRGARVEIGGNDGQTPIHCASSGGHLHVVQYLVSRGARVEIGGNDGQTPLHCASRNGHLDVVQYLVSRGAHF 203
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Match: Q16FJ0 (AAEL014741-PA (Fragment) OS=Aedes aegypti OX=7159 GN=AAEL014741 PE=4 SV=1) HSP 1 Score: 119.013 bits (297), Expect = 5.789e-28 Identity = 53/156 (33.97%), Postives = 88/156 (56.41%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DD G +H A G +EV +++ID +N++ G P+H AS G LE++ LL++ ++ N G P+H AS GH E++K L++ N++ N+G P+H AS GHL+++KLL+ + N + L +A Q+ ++ V+LLI Sbjct: 42 DDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLI 197 HSP 2 Score: 116.701 bits (291), Expect = 4.270e-27 Identity = 49/149 (32.89%), Postives = 87/149 (58.39%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +++ G++E+ + +ID +NI+ + G P+H AS G LE++ LL++ +++ N G P+H AS GH E++KLL++ N+ N G P+H AS GHL+++K L+ + +D N + L +A Q+ ++ V+LLI Sbjct: 16 VYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLI 164 HSP 3 Score: 114.775 bits (286), Expect = 2.472e-26 Identity = 46/131 (35.11%), Postives = 78/131 (59.54%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCK 576 ++G +H+A G +EV +++ID G+N+ G P+H AS G LE++ L++ +++ N G P+H AS GH E++KLL++ N+ +N G P+H AS GHL+++KLL+ + +D K Sbjct: 76 NKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTENEGWTPLHVASQNGHLEVVKLLIDNRANVDAK 206 HSP 4 Score: 87.0409 bits (214), Expect = 3.982e-16 Identity = 36/119 (30.25%), Postives = 70/119 (58.82%), Query Frame = 1 Query: 292 MRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 M+ ++ + G +E++ L++ N +I+ + ++G P+H AS GH E++KLL++ N++ N+G P+H AS GHL+++KLL+ + N + L +A + ++ V+ LI Sbjct: 13 MQLVYLTADNGYIEMVKFLIDHNANIDTKDDNGWTPLHRASQNGHLEVVKLLIDNRANVDTTQNKGWTPLHVASQNGHLEVVKLLIDNGANVYTTQNEGWTPLHVASLNGHLEVVKSLI 131
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Match: A0A4Q2V509 (ANK_REP_REGION domain-containing protein (Fragment) OS=Fusarium oxysporum f. sp. narcissi OX=451672 GN=BFJ63_vAg14992 PE=4 SV=1) HSP 1 Score: 116.316 bits (290), Expect = 2.108e-26 Identity = 54/159 (33.96%), Postives = 92/159 (57.86%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T+ D+ G ++ A G ++V +++++ G+NI G+ P++ AS G +E++ LLLE DI N G P++ AS GH E++KLLLE NI DN G+ P++ AS GH++++KLLL+ + I +N + + A ++ V+LL+ Sbjct: 23 TVADNDGWTPVYAASFDGHIDVVKLLLENGANITIVDNEGVTPVNAASLNGHVEVVKLLLENGADITVADNDGWTPVYAASFNGHVEVVKLLLENGANITVVDNEGVTPVNAASLNGHVEVVKLLLENEADITVGDNDGWTPVYAASDRGHVEVVKLLL 181 HSP 2 Score: 114.775 bits (286), Expect = 8.575e-26 Identity = 55/159 (34.59%), Postives = 90/159 (56.60%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 T+ D+ G ++ A G VEV +++++ G+NI G+ P++ AS G +E++ LLLE DI N G P++ AS GH E++KLLLE +I DN G+ P++ AS GH++++KLLL+ I +N + + A + D +LL+ Sbjct: 89 TVADNDGWTPVYAASFNGHVEVVKLLLENGANITVVDNEGVTPVNAASLNGHVEVVKLLLENEADITVGDNDGWTPVYAASDRGHVEVVKLLLENGADITVADNEGVMPVNAASFNGHVEVVKLLLENGADIMVGDNDGWTPVYAASFNGHADVAKLLL 247 HSP 3 Score: 114.005 bits (284), Expect = 1.828e-25 Identity = 55/159 (34.59%), Postives = 93/159 (58.49%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 TIVD++G ++ A + G VEV +++++ G++I G P++ AS G +E++ LLLE +I N G+ P++ AS GH E++KLLLE +I DN G P++ AS GH++++KLLL+ I +N + A + ++ V+LL+ Sbjct: 56 TIVDNEGVTPVNAASLNGHVEVVKLLLENGADITVADNDGWTPVYAASFNGHVEVVKLLLENGANITVVDNEGVTPVNAASLNGHVEVVKLLLENEADITVGDNDGWTPVYAASDRGHVEVVKLLLENGADITVADNEGVMPVNAASFNGHVEVVKLLL 214 HSP 4 Score: 108.997 bits (271), Expect = 1.280e-23 Identity = 51/137 (37.23%), Postives = 80/137 (58.39%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNN 582 T+VD++G ++ A + G VEV +++++ ++I G P++ AS RG +E++ LLLE DI N G+ P++ AS GH E++KLLLE +I DN G P++ AS GH + KLLL+ I +N Sbjct: 122 TVVDNEGVTPVNAASLNGHVEVVKLLLENEADITVGDNDGWTPVYAASDRGHVEVVKLLLENGADITVADNEGVMPVNAASFNGHVEVVKLLLENGADIMVGDNDGWTPVYAASFNGHADVAKLLLENGADITVADN 258 HSP 5 Score: 102.449 bits (254), Expect = 2.945e-21 Identity = 47/148 (31.76%), Postives = 85/148 (57.43%), Query Frame = 1 Query: 205 HIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + A G ++V +++++ G++I G P++ AS G ++++ LLLE +I N G+ P++ AS GH E++KLLLE +I DN G P++ AS GH++++KLLL+ I +N + + A + ++ V+LL+ Sbjct: 1 YAASFNGHIDVVKLLLENGADITVADNDGWTPVYAASFDGHIDVVKLLLENGANITIVDNEGVTPVNAASLNGHVEVVKLLLENGADITVADNDGWTPVYAASFNGHVEVVKLLLENGANITVVDNEGVTPVNAASLNGHVEVVKLLL 148 HSP 6 Score: 79.7221 bits (195), Expect = 3.703e-13 Identity = 38/106 (35.85%), Postives = 61/106 (57.55%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRG 489 T+ D+ G ++ A G VEV +++++ G++I G+ P++ AS G +E++ LLLE DI N G P++ AS GH+++ KLLLE +I DN G Sbjct: 155 TVGDNDGWTPVYAASDRGHVEVVKLLLENGADITVADNEGVMPVNAASFNGHVEVVKLLLENGADIMVGDNDGWTPVYAASFNGHADVAKLLLENGADITVADNNG 260
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Match: ank3b (ankyrin 3b [Source:ZFIN;Acc:ZDB-GENE-060621-2]) HSP 1 Score: 107.842 bits (268), Expect = 8.508e-25 Identity = 62/209 (29.67%), Postives = 108/209 (51.67%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + + P+H AS G+S ++KLLLER I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ + E+ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 152 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------HHEVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 339 HSP 2 Score: 91.6633 bits (226), Expect = 1.707e-19 Identity = 46/166 (27.71%), Postives = 88/166 (53.01%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + + P+H AS RG ++ LLLER I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLL + +D N + L +A ++++ Sbjct: 169 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIV 334 HSP 3 Score: 80.8777 bits (198), Expect = 5.705e-16 Identity = 40/150 (26.67%), Postives = 80/150 (53.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + + RG +H A+ G +++K L+Q +D K+ ++ L ++ + + + +Q L+ Sbjct: 317 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLL 466 HSP 4 Score: 78.5666 bits (192), Expect = 3.261e-15 Identity = 44/165 (26.67%), Postives = 81/165 (49.09%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G+ +H+A G +VA I++D G+++ T G P+H A+ G +E+ LLL++ + SG+ P+H A+ + ++ LLL++ + G P+H A+ ++I LL+ + S L LA Q +D V LL++ + N++ Sbjct: 479 GYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKS 643 HSP 5 Score: 75.8702 bits (185), Expect = 2.084e-14 Identity = 34/135 (25.19%), Postives = 74/135 (54.81%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSES 594 G+ +HIA +E+A +++ G++ T G+ P+H AS G ++++TLL+ R+ I SG+ P+H A+ + ++L+ ++ E G P+H A G+++++ LL+ + +++ K ++ + Sbjct: 578 GYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVTAA 712 HSP 6 Score: 75.8702 bits (185), Expect = 2.179e-14 Identity = 39/154 (25.32%), Postives = 76/154 (49.35%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G EV + ++ G+ ++ ++ P+H +S G EI+ LL+ + T SG P+H A+ GH ++ +LL++ ++ +G P+H A+ G +++ LLLQ++ D + L +A LL+ Sbjct: 412 RGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLL 565 HSP 7 Score: 74.7146 bits (182), Expect = 5.722e-14 Identity = 41/156 (26.28%), Postives = 76/156 (48.72%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A +VA +++D G++ G P+H A+ + +EI T LLE D T G+ P+H AS G+ +++ LL+ R+ I + G+ P+H A+ + + ++L+ +D + M + L +A + V L+ Q Sbjct: 545 GLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQ 700 HSP 8 Score: 74.3294 bits (181), Expect = 6.963e-14 Identity = 36/149 (24.16%), Postives = 82/149 (55.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + +++++ G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 219 LHVASKRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLL 367 HSP 9 Score: 72.7886 bits (177), Expect = 2.181e-13 Identity = 45/178 (25.28%), Postives = 83/178 (46.63%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +EG+ D L I +G +H+A G +EVA +++ + + G+ P+H A+ ++ LLL++ +G P+H A+ EI LLE + +G+ P+H AS G++ ++ LL+ R I+ N + L LA Q Q++ ++L++ Sbjct: 488 REGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLLEYGADTNAITRQGISPLHLASQEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVN 665 HSP 10 Score: 70.8626 bits (172), Expect = 1.082e-12 Identity = 47/206 (22.82%), Postives = 89/206 (43.20%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC-----------------------------NIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 KEG+ + V + +K +G+ ++HIA + G +V + ++ G+N+ ++ G P++ A+ L+++ LL+ T G P+ A GH +++ LLLE + N + E G P+H A+ G++ + LLL R +D K + L +A + + V+LL+ Sbjct: 30 KEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLENDTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVKLLL 235 HSP 11 Score: 68.1662 bits (165), Expect = 6.845e-12 Identity = 39/153 (25.49%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I+ T G+ PIH A+ G I+T L+ G +H A+ G +E++K L++ ++ + P+H +S G +I++ LLQ D + L LA + D +L+ Sbjct: 347 GFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILL 499 HSP 12 Score: 67.3958 bits (163), Expect = 1.228e-11 Identity = 42/167 (25.15%), Postives = 77/167 (46.11%), Query Frame = 1 Query: 148 YLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 YLKS I + G ++H+A G VEV +I G+ ++ T G +H AS G +++ L+ ++ ++ +G P++ A+ H ++++ LL+ + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 7 YLKSGVDINICNQNGLNALHLASKEGHVEVVTQLIKMGATVDAATKKGNTALHIASLAGQSDVVKELVTNGANVNAQSQNGFTPLYMAAQENHLDVVRYLLDNGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 169 HSP 13 Score: 66.6254 bits (161), Expect = 2.087e-11 Identity = 51/224 (22.77%), Postives = 94/224 (41.96%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDE 690 K GN++ V L+ G +H G +V +++D G+ I +T G+ P+H A+ L + LLL ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+ L+ + N E+ L +A ++ Q + V+ L+ D +++D+ Sbjct: 224 KRGNSNMVKLLLERGARIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHEVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRIKVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQ 447 HSP 14 Score: 63.5438 bits (153), Expect = 2.279e-10 Identity = 39/156 (25.00%), Postives = 74/156 (47.44%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +HI+ G E+ + ++ G+ + TT G P+H A+ G ++ ++LL++ + T G P+H A+ G E+ LLL++ + G+ P+H A+ + ++ LLL + + L +A + QM+ L+ Sbjct: 445 DDQT--PLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLLQKRAPPDASGKSGLTPLHVAAHYDNQKVALLLLDQGASPHAAAKNGYTPLHIAAKKNQMEIATTLL 598 HSP 15 Score: 56.6102 bits (135), Expect = 3.258e-8 Identity = 33/157 (21.02%), Postives = 70/157 (44.59%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G + +++ G++ G +H A+ G E++ L++ ++ ++ P+H +S G EI++ LL+ + G P+H A+ GH + +LL + + + L +A + +++ LL+ Sbjct: 376 VTESGLTPIHVAAFMGHENIVTQLMNHGASPNTTNVRGETALHMAARAGQTEVVKYLVQNGAYVDAKSKDDQTPLHISSRLGKPEIIQQLLQHGACPDSTTTSGYTPLHLAAREGHKDVASILLDQGASLGITTKKGFTPLHVAAKYGKIEVANLLL 532 HSP 16 Score: 51.9878 bits (123), Expect = 8.336e-7 Identity = 30/112 (26.79%), Postives = 55/112 (49.11%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLE 453 +EGN D V + D + + G +H+A V VA ++++ G+ ++ ET G P+H A G ++++ LL+ + +T A+ GH+ I+ LLL+ Sbjct: 620 QEGNVDMVTLLMARDATINLCNKSGLTPLHLAAQEDQVNVAEVLVNHGATVDPETKMGYTPLHVACHYGNVKMVHFLLKNQAKVNAKTKVT------AAQQGHTHIINLLLQ 725
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Match: ENSAMXT00000037173.1 (pep primary_assembly:Astyanax_mexicanus-2.0:16:21074007:21119253:-1 gene:ENSAMXG00000017269.2 transcript:ENSAMXT00000037173.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 98.9821 bits (245), Expect = 2.354e-22 Identity = 57/209 (27.27%), Postives = 103/209 (49.28%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + ++ G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ +++LLL+R I+ + G+ P+HCA+ GH ++LLL+R I + S L +A Q ++CV+ L+ + D++ D + + LHV A C R+ K +LDK + Sbjct: 179 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPV------DDVTLDYL------------TALHV---AAHCGHYRVTKLLLDKRAN 366 HSP 2 Score: 97.8265 bits (242), Expect = 4.751e-22 Identity = 48/140 (34.29%), Postives = 78/140 (55.71%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G V VA ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH ++LLLER I G+ P+H A+ G H++ +K LLQ K +D Sbjct: 196 LQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 335 HSP 3 Score: 85.8853 bits (211), Expect = 5.270e-18 Identity = 63/254 (24.80%), Postives = 111/254 (43.70%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFI---------NRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 FL+ + GN D V +YLK + G ++H+A G +++ + +++ GS+++ T G +H AS G E++ +L+++ DI ++ +G P++ AS H ++++ LLE N G P+ A GH Q++ +LL+ D K + L +A + LL+ VQ F N N + DF N + LHV N +V+ +LD+ Sbjct: 18 SFLRAARAGNIDKVLEYLKGGVDIGTSNQNGLNALHLAAKEGHMDLVQELLERGSSVDSATKKGNTALHIASLAGQAEVVKILVKQGADINAQSQNGFTPLYMASQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTN---MVRLLLDR 264 HSP 4 Score: 80.1073 bits (196), Expect = 4.275e-16 Identity = 44/153 (28.76%), Postives = 81/153 (52.94%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G + R+++D GS I+ +T G+ P+H A+ G + LLLER I T +G+ P+H A+ G H E +K LL+ ++ + +H A+ GH ++ KLLL ++ + + + L +A + ++ ++LL+ Sbjct: 242 GITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHDTAVELLLERGAPILARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLV 394 HSP 5 Score: 56.9954 bits (136), Expect = 1.546e-8 Identity = 27/95 (28.42%), Postives = 50/95 (52.63%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDN 483 ++H+A G V ++++D +N G P+H A + ++++ LL++ I+ T SG+ PIH A+ GH I+ LLL+ + + N Sbjct: 344 ALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTAN 438
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 100.138 bits (248), Expect = 3.009e-22 Identity = 58/209 (27.75%), Postives = 105/209 (50.24%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H A+ G+ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ ++ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 207 ALHIAARKDDTKSAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHDVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 394 HSP 2 Score: 89.3521 bits (220), Expect = 1.111e-18 Identity = 45/166 (27.11%), Postives = 87/166 (52.41%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ G+ ++ + P+H A+ RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 224 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIV 389 HSP 3 Score: 85.5001 bits (210), Expect = 1.814e-17 Identity = 43/150 (28.67%), Postives = 80/150 (53.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G +++ LLQ ++D K ++ L +A + + + VQLL+ Sbjct: 372 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLL 521 HSP 4 Score: 79.337 bits (194), Expect = 1.769e-15 Identity = 44/154 (28.57%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G +V R ++ G+ ++ + +H AS G EI+ LLL++ T SG P+H A GH E LLLE ++ +G P+H A+ G ++ KLLLQ++ D + L +A LL+ Sbjct: 467 RGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLL 620 HSP 5 Score: 77.0258 bits (188), Expect = 8.974e-15 Identity = 37/149 (24.83%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ + ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 274 LHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 422 HSP 6 Score: 77.0258 bits (188), Expect = 9.385e-15 Identity = 35/153 (22.88%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL +N ++ +G+ P+H A+ + ++LL ++ G P+H A G+++++ LL+ + +I+ K + L A Q + +L+ Sbjct: 633 GYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLL 785 HSP 7 Score: 74.7146 bits (182), Expect = 5.515e-14 Identity = 42/156 (26.92%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL +N N+ + G+ P+H A+ + + ++LL +D + L +A M V L+ Q Sbjct: 600 GLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQ 755 HSP 8 Score: 74.7146 bits (182), Expect = 5.615e-14 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VEV ++ G++++ T G +H AS G +++ L+ ++ ++ +G P++ A+ H E+++ LLE + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 47 YLRAARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 224 HSP 9 Score: 73.9442 bits (180), Expect = 1.136e-13 Identity = 47/187 (25.13%), Postives = 84/187 (44.92%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G A+ V L+ + G+ +H+A G E A ++++ G+++ T G P+H A+ G +E LLL++ + +G+ P+H A+ + + LLL+ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ + N+N Sbjct: 512 GKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKN 698 HSP 10 Score: 71.2478 bits (173), Expect = 6.340e-13 Identity = 34/127 (26.77%), Postives = 64/127 (50.39%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRK 558 V QG +H+A G V++ +++ +N+ G+ P+H A+ + + +LL +++ T +G P+H A G+ +++ LLE I + G P+H A+ GH I+ +LLQ + Sbjct: 662 VTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHR 788 HSP 11 Score: 70.8626 bits (172), Expect = 9.888e-13 Identity = 49/203 (24.14%), Postives = 88/203 (43.35%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ + ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q D V+ L+ D ++D+ A Sbjct: 303 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTAL 505 HSP 12 Score: 68.1662 bits (165), Expect = 7.880e-12 Identity = 45/171 (26.32%), Postives = 75/171 (43.86%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMD 630 + G AD V L++ I ++HIA G E+ ++++ G+ TT G P+H A G E +LLLE + T G P+H A+ G E KLLL++ + G+ P+H A+ + ++ LLL + L +A + Q++ Sbjct: 477 RAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLE 647 HSP 13 Score: 64.6994 bits (156), Expect = 9.773e-11 Identity = 37/143 (25.87%), Postives = 70/143 (48.95%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 +EG+ D V L + + + G +H+A V VA ++++ G+ ++ T G P+H A G +++++ LLE I +T SG P+H A+ GH+ I+ +LL+ + G + A G++ ++ L Sbjct: 675 QEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 817 HSP 14 Score: 63.929 bits (154), Expect = 1.479e-10 Identity = 37/153 (24.18%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G +++++ LL+ ++ + +H AS G +I++LLLQ+ + L LA++ + LL+ Sbjct: 402 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLL 554 HSP 15 Score: 62.7734 bits (151), Expect = 3.413e-10 Identity = 36/155 (23.23%), Postives = 71/155 (45.81%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +G +H+A G +E A++++ + + G+ P+H A+ + LLL+ +G P+H A+ EI LLE +G+ P+H A+ G + ++ LLL + ++ N + L LA Q ++ ++L++ Sbjct: 566 KGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLN 720 HSP 16 Score: 60.4622 bits (145), Expect = 2.276e-9 Identity = 36/157 (22.93%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G + + + G++ G +H A+ G +++ LL+ ++ + +H AS G +EI++LLL++ G P+H A GH + LLL+ + + L +A + Q++ +LL+ Sbjct: 431 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLL 587
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 93.5893 bits (231), Expect = 4.016e-20 Identity = 59/217 (27.19%), Postives = 105/217 (48.39%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECE--------TTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E T G P+H A+ G + + TLLL R ++ + + P+H A+ G+ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ ++ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 171 ALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHDVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 366 HSP 2 Score: 87.8113 bits (216), Expect = 3.226e-18 Identity = 43/153 (28.10%), Postives = 82/153 (53.59%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIA G + VA ++++ G+ ++ + P+H A+ RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 209 GFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIV 361 HSP 3 Score: 85.5001 bits (210), Expect = 1.837e-17 Identity = 43/150 (28.67%), Postives = 80/150 (53.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G +++ LLQ ++D K ++ L +A + + + VQLL+ Sbjct: 344 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLL 493 HSP 4 Score: 79.337 bits (194), Expect = 1.791e-15 Identity = 44/154 (28.57%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G +V R ++ G+ ++ + +H AS G EI+ LLL++ T SG P+H A GH E LLLE ++ +G P+H A+ G ++ KLLLQ++ D + L +A LL+ Sbjct: 439 RGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLL 592 HSP 5 Score: 77.0258 bits (188), Expect = 9.083e-15 Identity = 37/149 (24.83%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ + ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 246 LHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 394 HSP 6 Score: 77.0258 bits (188), Expect = 9.499e-15 Identity = 35/153 (22.88%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL +N ++ +G+ P+H A+ + ++LL ++ G P+H A G+++++ LL+ + +I+ K + L A Q + +L+ Sbjct: 605 GYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLL 757 HSP 7 Score: 74.7146 bits (182), Expect = 5.581e-14 Identity = 42/156 (26.92%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL +N N+ + G+ P+H A+ + + ++LL +D + L +A M V L+ Q Sbjct: 572 GLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQ 727 HSP 8 Score: 74.7146 bits (182), Expect = 5.681e-14 Identity = 45/182 (24.73%), Postives = 85/182 (46.70%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +L+ + GN + YLK+ I + G ++H+A G VEV ++ G++++ T G +H AS G +++ L+ ++ ++ +G P++ A+ H E+++ LLE + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 11 YLRAARAGNLEKALDYLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 188 HSP 9 Score: 73.9442 bits (180), Expect = 1.150e-13 Identity = 47/187 (25.13%), Postives = 84/187 (44.92%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G A+ V L+ + G+ +H+A G E A ++++ G+++ T G P+H A+ G +E LLL++ + +G+ P+H A+ + + LLL+ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ + N+N Sbjct: 484 GKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKN 670 HSP 10 Score: 71.2478 bits (173), Expect = 6.412e-13 Identity = 34/127 (26.77%), Postives = 64/127 (50.39%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRK 558 V QG +H+A G V++ +++ +N+ G+ P+H A+ + + +LL +++ T +G P+H A G+ +++ LLE I + G P+H A+ GH I+ +LLQ + Sbjct: 634 VTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHR 760 HSP 11 Score: 70.8626 bits (172), Expect = 1.000e-12 Identity = 49/203 (24.14%), Postives = 88/203 (43.35%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ + ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q D V+ L+ D ++D+ A Sbjct: 275 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTAL 477 HSP 12 Score: 68.1662 bits (165), Expect = 7.966e-12 Identity = 45/171 (26.32%), Postives = 75/171 (43.86%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMD 630 + G AD V L++ I ++HIA G E+ ++++ G+ TT G P+H A G E +LLLE + T G P+H A+ G E KLLL++ + G+ P+H A+ + ++ LLL + L +A + Q++ Sbjct: 449 RAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLE 619 HSP 13 Score: 64.6994 bits (156), Expect = 9.533e-11 Identity = 48/196 (24.49%), Postives = 90/196 (45.92%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL-------------LQRKCRIDCKNNMSESILELAIQSKQMDCV-QLLISVQEL 663 +EG+ D V L + + + G +H+A V VA ++++ G+ ++ T G P+H A G +++++ LLE I +T SG P+H A+ GH+ I+ +LL+ + G + A G++ ++ L + K +++ M+E L+++ Q D + Q L S Q L Sbjct: 647 QEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTLRGLTDENLTATPPVSEKHKLNVPETMNE-FLDMSDDEVQPDLIGQDLCSAQPL 841 HSP 14 Score: 63.929 bits (154), Expect = 1.494e-10 Identity = 37/153 (24.18%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G +++++ LL+ ++ + +H AS G +I++LLLQ+ + L LA++ + LL+ Sbjct: 374 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLL 526 HSP 15 Score: 62.7734 bits (151), Expect = 3.448e-10 Identity = 36/155 (23.23%), Postives = 71/155 (45.81%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +G +H+A G +E A++++ + + G+ P+H A+ + LLL+ +G P+H A+ EI LLE +G+ P+H A+ G + ++ LLL + ++ N + L LA Q ++ ++L++ Sbjct: 538 KGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLN 692 HSP 16 Score: 60.4622 bits (145), Expect = 2.298e-9 Identity = 36/157 (22.93%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G + + + G++ G +H A+ G +++ LL+ ++ + +H AS G +EI++LLL++ G P+H A GH + LLL+ + + L +A + Q++ +LL+ Sbjct: 403 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLL 559
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Match: ank3a (ankyrin 3a [Source:ZFIN;Acc:ZDB-GENE-060621-1]) HSP 1 Score: 93.5893 bits (231), Expect = 4.398e-20 Identity = 59/217 (27.19%), Postives = 105/217 (48.39%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECE--------TTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E T G P+H A+ G + + TLLL R ++ + + P+H A+ G+ ++KLLL+R I+ + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ ++ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 151 ALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------QHDVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 346 HSP 2 Score: 87.8113 bits (216), Expect = 3.524e-18 Identity = 43/153 (28.10%), Postives = 82/153 (53.59%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIA G + VA ++++ G+ ++ + P+H A+ RG ++ LLL+R I+ +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A ++++ Sbjct: 189 GFTPLHIAAHYGNINVATLLLNRGAAVDFMARNDITPLHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIV 341 HSP 3 Score: 85.5001 bits (210), Expect = 2.005e-17 Identity = 43/150 (28.67%), Postives = 80/150 (53.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G +VA++I+D +N + G P+H A + ++++ LLL+ ++ T SG+ PIH A+ GH I+K L + + RG +H A+ G +++ LLQ ++D K ++ L +A + + + VQLL+ Sbjct: 324 ALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLL 473 HSP 4 Score: 79.337 bits (194), Expect = 1.947e-15 Identity = 44/154 (28.57%), Postives = 72/154 (46.75%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G ++H+A G +V R ++ G+ ++ + +H AS G EI+ LLL++ T SG P+H A GH E LLLE ++ +G P+H A+ G ++ KLLLQ++ D + L +A LL+ Sbjct: 419 RGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLL 572 HSP 5 Score: 77.0258 bits (188), Expect = 9.860e-15 Identity = 37/149 (24.83%), Postives = 84/149 (56.38%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + ++++D G+ I+ +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ + ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 226 LHVAAKRGNGNMVKLLLDRGAKIDAKTKDGLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 374 HSP 6 Score: 77.0258 bits (188), Expect = 1.031e-14 Identity = 35/153 (22.88%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+ +++ G+ T G+ P+H A+ G +++++LLL +N ++ +G+ P+H A+ + ++LL ++ G P+H A G+++++ LL+ + +I+ K + L A Q + +L+ Sbjct: 585 GYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLL 737 HSP 7 Score: 74.7146 bits (182), Expect = 6.048e-14 Identity = 42/156 (26.92%), Postives = 73/156 (46.79%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H A+ G +++ LLL +N N+ + G+ P+H A+ + + ++LL +D + L +A M V L+ Q Sbjct: 552 GLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQ 707 HSP 8 Score: 73.9442 bits (180), Expect = 1.245e-13 Identity = 47/187 (25.13%), Postives = 84/187 (44.92%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G A+ V L+ + G+ +H+A G E A ++++ G+++ T G P+H A+ G +E LLL++ + +G+ P+H A+ + + LLL+ + G P+H A+ L+I LL+ + S L LA Q +D V LL++ + N+N Sbjct: 464 GKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKN 650 HSP 9 Score: 71.2478 bits (173), Expect = 6.931e-13 Identity = 34/127 (26.77%), Postives = 64/127 (50.39%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRK 558 V QG +H+A G V++ +++ +N+ G+ P+H A+ + + +LL +++ T +G P+H A G+ +++ LLE I + G P+H A+ GH I+ +LLQ + Sbjct: 614 VTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHR 740 HSP 10 Score: 70.8626 bits (172), Expect = 1.080e-12 Identity = 49/203 (24.14%), Postives = 88/203 (43.35%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H G +V +++D G+ I +T G+ P+H A+ L + LLL+ + ++ TN + +H A+ GH ++ K+++++ N I C+ NR G+ PIH A+ GH I+K L + N E+ L +A ++ Q D V+ L+ D ++D+ A Sbjct: 255 GLTPLHCGARSGHEQVVEMLLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTAL 457 HSP 11 Score: 70.8626 bits (172), Expect = 1.191e-12 Identity = 51/201 (25.37%), Postives = 90/201 (44.78%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRG---MRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSE--------SILELAIQSKQMDCVQLLISVQELFDFINRND 687 KEG+ + V + LK +G+ ++HIA + G +V R ++ G+N+ ++ G P++ A+ LE++ LLE T G P+ A GH +++ LLLE D +G + +H A+ + LLLQ D ++ + + L +A ++ LL++ DF+ RND Sbjct: 29 KEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLE-------NDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVESKVHTYTHTHSGFTPLHIAAHYGNINVATLLLNRGAAVDFMARND 222 HSP 12 Score: 69.3218 bits (168), Expect = 3.607e-12 Identity = 42/167 (25.15%), Postives = 78/167 (46.71%), Query Frame = 1 Query: 148 YLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 YLK+ I + G ++H+A G VEV ++ G++++ T G +H AS G +++ L+ ++ ++ +G P++ A+ H E+++ LLE + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 6 YLKTGVDINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHIASLAGQTDVVRELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLEHGASQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKSAALLL 168 HSP 13 Score: 68.1662 bits (165), Expect = 8.586e-12 Identity = 45/171 (26.32%), Postives = 75/171 (43.86%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMD 630 + G AD V L++ I ++HIA G E+ ++++ G+ TT G P+H A G E +LLLE + T G P+H A+ G E KLLL++ + G+ P+H A+ + ++ LLL + L +A + Q++ Sbjct: 429 RAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLE 599 HSP 14 Score: 64.6994 bits (156), Expect = 1.061e-10 Identity = 37/143 (25.87%), Postives = 70/143 (48.95%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL 546 +EG+ D V L + + + G +H+A V VA ++++ G+ ++ T G P+H A G +++++ LLE I +T SG P+H A+ GH+ I+ +LL+ + G + A G++ ++ L Sbjct: 627 QEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLNHGAEVDAHTKTGYTPLHVACHYGNMKMVSFLLENQAKINSKTKSGYTPLHQAAQQGHTHIINMLLQHRASANELTLNGNTALSIARRLGYISVVDTL 769 HSP 15 Score: 63.929 bits (154), Expect = 1.604e-10 Identity = 37/153 (24.18%), Postives = 70/153 (45.75%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++++ T G+ PIH A+ G I+ L G +H A+ G +++++ LL+ ++ + +H AS G +I++LLLQ+ + L LA++ + LL+ Sbjct: 354 GFTPLHIACKKNRVKVMELLLKHGASLQAVTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLL 506 HSP 16 Score: 62.7734 bits (151), Expect = 3.698e-10 Identity = 36/155 (23.23%), Postives = 71/155 (45.81%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +G +H+A G +E A++++ + + G+ P+H A+ + LLL+ +G P+H A+ EI LLE +G+ P+H A+ G + ++ LLL + ++ N + L LA Q ++ ++L++ Sbjct: 518 KGFTPLHVAAKYGQIEAAKLLLQKRAAPDTAGKTGLTPLHVAAHYDNQRVALLLLDHGASPHAAAKNGYTPLHIAAKKNQLEIGTTLLEFGAETNAVTRQGISPLHLAAQEGSVDLVSLLLTKNANVNMGNKNGLTPLHLAAQEDRVGVAEVLLN 672 HSP 17 Score: 60.4622 bits (145), Expect = 2.458e-9 Identity = 36/157 (22.93%), Postives = 69/157 (43.95%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G IH+A G + + + G++ G +H A+ G +++ LL+ ++ + +H AS G +EI++LLL++ G P+H A GH + LLL+ + + L +A + Q++ +LL+ Sbjct: 383 VTESGLTPIHVAAFMGHENIVKQLTHHGASPNTTNVRGETALHMAARAGQADVVRYLLQNGAKVDIKAKDDQTALHIASRLGKAEIVQLLLQKGAWASAATTSGYTPLHLAVREGHQETASLLLEHGASLSAATKKGFTPLHVAAKYGQIEAAKLLL 539
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000095.1 (pep scaffold:Pmarinus_7.0:GL477582:20897:60453:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000095.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 102.834 bits (255), Expect = 6.211e-24 Identity = 58/206 (28.16%), Postives = 102/206 (49.51%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA A +++ N + + G P+H A+ G + + TLLL ++ +G+ P+H A+ G++ ++KLLL+R I+ + G+ P+HCA+ GH +++++LL+R I K S L +A Q + + CVQLL+ Q D++ D + + LHV A C R+ K +LD+ Sbjct: 175 ALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV------DDVTLDYL------------TPLHV---AAHCGHYRVAKLLLDR 359 HSP 2 Score: 98.2117 bits (243), Expect = 2.048e-22 Identity = 48/149 (32.21%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G VA++++D G G P+H A + ++++ LLL+ I T SG+ PIH AS GH +++LLL+ + + RG P+H AS G L+++K LLQ R+ K ++ L +A + + + VQLL+ Sbjct: 341 LHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLL 489 HSP 3 Score: 97.8265 bits (242), Expect = 2.507e-22 Identity = 43/132 (32.58%), Postives = 78/132 (59.09%), Query Frame = 1 Query: 175 IVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 + G +HIA G + VA ++++ G+ ++ G+ P+H A+ RG ++ LLL+R I+ +T G+ P+H A+ GH +++++LLER+ I + G+ P+H A+ G HL ++LLL R+ +D Sbjct: 200 VTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVD 331 HSP 4 Score: 92.4337 bits (228), Expect = 1.358e-20 Identity = 47/151 (31.13%), Postives = 78/151 (51.66%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCK 576 G+ + V L++ + + +G +H+A G +EV + ++ G+ + + P+H AS G EI+ LLL+R D T SG P+++AS GH ++ LLLE +I +G P+H AS G L++ LLL++ D K Sbjct: 414 GHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPK 564 HSP 5 Score: 84.7297 bits (208), Expect = 5.658e-18 Identity = 46/153 (30.07%), Postives = 72/153 (47.06%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC ++V +++ G++I T G+ PIH AS G L ++ LLL+ G P+H AS G E++K LL+ + + P+H AS G +I++LLLQR + + L A + D LL+ Sbjct: 370 GFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLL 522 HSP 6 Score: 82.0333 bits (201), Expect = 3.681e-17 Identity = 56/232 (24.14%), Postives = 97/232 (41.81%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERN-------------CNIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 +H+A G + ++++D G+ I+ +T G+ P+H A+ G +++ +LLER+ I +T +G+ P+H A+ G GH + KLLL+R +I C+ NR G+ PIH AS GHL +++LLLQ + N E+ L +A ++ Q++ V+ L+ R D+ Sbjct: 242 LHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPL 473 HSP 7 Score: 79.337 bits (194), Expect = 2.853e-16 Identity = 64/266 (24.06%), Postives = 112/266 (42.11%), Query Frame = 1 Query: 79 FMMGDFQC-----FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD---------------FINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 F FQ FL+ + GN + + +LK + G ++H+A G V+V ++ + I+ T G +H A+ G +I+ +L+E +I ++ +G P++ A+ H EI+K LL+ N G P+ A GH Q++ LLL+ D K + L +A + + LL+ + D + N N + + DF N + LHV A + + +VK +LD+ Sbjct: 2 FFALSFQSDAATSFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLES----DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKSGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHV---AAKRGNNNMVKLLLDR 260 HSP 8 Score: 77.7962 bits (190), Expect = 1.024e-15 Identity = 42/155 (27.10%), Postives = 74/155 (47.74%), Query Frame = 1 Query: 184 DQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + G IH+A G + V ++++ G++ G P+H AS G LE++ LL+ + + P+H AS G +EI++LLL+R + G P++ AS GH + LLL+ I + L +A + +++ LL+ Sbjct: 401 ESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLL 555 HSP 9 Score: 76.2554 bits (186), Expect = 2.901e-15 Identity = 43/180 (23.89%), Postives = 85/180 (47.22%), Query Frame = 1 Query: 145 KYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 KY +D + G +H+A +VA +++D G++ G P+H A+ + LE+ +LLE + T G+ P+H A+ GH++++ LLL R+ +I G+ +H A+ + + ++L + K ++ + + L +A + V L+ + L + RN Sbjct: 557 KYASAD--PKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRN 734 HSP 10 Score: 73.9442 bits (180), Expect = 1.665e-14 Identity = 44/178 (24.72%), Postives = 84/178 (47.19%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIID--CGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 +EG+ D L++ + V +G +HIA G +EVA ++++ ++ + G+ P+H A+ ++ LLL++ +G P+H A+ E+ K+LLE +G+ P+H A+ GH ++ LLL R I + + LA Q +++ ++L Sbjct: 511 REGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKVAGQNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 688 HSP 11 Score: 72.7886 bits (177), Expect = 4.334e-14 Identity = 38/170 (22.35%), Postives = 78/170 (45.88%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G+ +HIA +EV +++++ G+ T G+ P+H A+ G +++ LLL R+ I T G+ +H A+ + ++L + ++ P+H AS G+++++ LL+ + ++ K + L A Q V L+ + + + N A Sbjct: 603 GYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTAL 772 HSP 12 Score: 55.4546 bits (132), Expect = 1.362e-8 Identity = 30/116 (25.86%), Postives = 56/116 (48.28%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+AD V L ++ G ++H+A V VA I+ +++ T P+H AS G ++++ LL + +T +G P+H A+ GH+ ++ LL+ + Sbjct: 645 QEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEAS 760
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000000093.1 (pep scaffold:Pmarinus_7.0:GL477582:20502:66045:1 gene:ENSPMAG00000000080.1 transcript:ENSPMAT00000000093.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 99.7525 bits (247), Expect = 5.533e-23 Identity = 53/156 (33.97%), Postives = 89/156 (57.05%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 LQH E NAD K + LN + G +HIA G + VA ++++ G+ ++ G+ P+H A+ RG ++ LLL+R I+ +T G+ P+H A+ GH +++++LLER+ I + G+ P+H A+ G HL ++LLL R+ +D Sbjct: 190 LLQH--EHNADVTSKMM----LNQTTES-GFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVD 338 HSP 2 Score: 97.8265 bits (242), Expect = 2.686e-22 Identity = 48/149 (32.21%), Postives = 80/149 (53.69%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G VA++++D G G P+H A + ++++ LLL+ I T SG+ PIH AS GH +++LLL+ + + RG P+H AS G L+++K LLQ R+ K ++ L +A + + + VQLL+ Sbjct: 348 LHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLL 496 HSP 3 Score: 96.6709 bits (239), Expect = 5.493e-22 Identity = 54/179 (30.17%), Postives = 90/179 (50.28%), Query Frame = 1 Query: 280 TTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 T G P+H A+ G + + TLLL ++ +G+ P+H A+ G++ ++KLLL+R I+ + G+ P+HCA+ GH +++++LL+R I K S L +A Q + + CVQLL+ Q D++ D + LHV A C R+ K +LD+ Sbjct: 209 TESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTARNGITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPV------DDVTLDYLTP------------LHV---AAHCGHYRVAKLLLDR 366 HSP 4 Score: 94.7449 bits (234), Expect = 2.936e-21 Identity = 53/183 (28.96%), Postives = 87/183 (47.54%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 F+ HL + V L++ + + +G +H+A G +EV + ++ G+ + + P+H AS G EI+ LLL+R D T SG P+++AS GH ++ LLLE +I +G P+H AS G L++ LLL++ D K + L +A LL+ Sbjct: 418 SFMGHL-----NVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLL 595 HSP 5 Score: 85.1149 bits (209), Expect = 3.773e-18 Identity = 45/153 (29.41%), Postives = 77/153 (50.33%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G ++ A G +VA ++++ G++I T G P+H AS G LE+ TLLLE+ + + +G+ P+H A+ H ++ LLL++ + G P+H A+ L++ K+LL+ + + L LA Q D V LL+ Sbjct: 509 GSTPLYFASREGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLL 661 HSP 6 Score: 84.7297 bits (208), Expect = 5.275e-18 Identity = 66/280 (23.57%), Postives = 113/280 (40.36%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYS---------------GLHVLDMAIECNADRIVKCILDKTVDDEEPKD---TNDHKDSEKNEDDKLRGICNICLEY---------PVDIAFQCGH 948 G +HIAC ++V +++ G++I T G+ PIH AS G L ++ LLL+ G P+H AS G E++K LL+ + + P+H AS G +I++LLLQR + + L A + D LL+ + + F + +S + R + + GL L +A + ++ +LDK T H ++KN+ + + + + E P+ +A Q GH Sbjct: 377 GFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPLHIASRLGKAEIVQLLLQRGSDPNAPTASGSTPLYFASREGHGDVASLLLENGADISPVTKK---GFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGH 653 HSP 7 Score: 82.0333 bits (201), Expect = 3.789e-17 Identity = 57/236 (24.15%), Postives = 98/236 (41.53%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERN-------------CNIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G +H+A G + ++++D G+ I+ +T G+ P+H A+ G +++ +LLER+ I +T +G+ P+H A+ G GH + KLLL+R +I C+ NR G+ PIH AS GHL +++LLLQ + N E+ L +A ++ Q++ V+ L+ R D+ Sbjct: 245 GITPLHVAAKRGNNNMVKLLLDRGAKIDTKTRDGLTPLHCAARSGHEKVIEMLLERDAPILAKTKNGLSPLHMATQGEHLACVQLLLGRQAPVDDVTLDYLTPLHVAAHCGHYRVAKLLLDRGGEPNARALNGFTPLHIACKKNRIKVMELLLKHGASIHTTTESGLTPIHVASFMGHLNVVQLLLQNGASSNATNVRGETPLHMASRAGQLEVVKTLLQSGARVGVKAREDQTPL 480 HSP 8 Score: 79.337 bits (194), Expect = 2.988e-16 Identity = 56/234 (23.93%), Postives = 105/234 (44.87%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFD-----FINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNA 786 FL+ + GN + + +LK + G ++H+A G V+V ++ + I+ T G +H A+ G +I+ +L+E +I ++ +G P++ A+ H EI+K LL+ N G P+ A GH Q++ LLL+ D K + L +A + + LL+ + D +N+ E F + ++ + N+ + L L A++ A Sbjct: 13 SFLRAARNGNLERLMDHLKGGMDINTCNQNGLNALHLASKEGHVDVVTELLKREAEIDTATKVGNTALHIAALAGQTDIVRVLVEFGANINAQSQNGFTPLYMAAQENHLEIVKFLLDNGANQSVATEDGFTPLAVALQQGHEQVVGLLLES----DTKGKVRLPALHIAARKDDTNAAALLLQHEHNADVTSKMMLNQTTESGFTPLHIAAHYGNMNVATLLLNLGAAVDFTA 242 HSP 9 Score: 76.6406 bits (187), Expect = 2.101e-15 Identity = 44/176 (25.00%), Postives = 85/176 (48.30%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLL 645 +EG+ D L++ + V +G +HIA G +EVA ++++ ++ + + G+ P+H A+ ++ LLL++ +G P+H A+ E+ K+LLE +G+ P+H A+ GH ++ LLL R I + + LA Q +++ ++L Sbjct: 518 REGHGDVASLLLENGADISPVTKKGFTPLHIASKYGRLEVATLLLEKYASADPKGKNGLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEIL 693 HSP 10 Score: 73.9442 bits (180), Expect = 1.536e-14 Identity = 40/165 (24.24%), Postives = 80/165 (48.48%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G +H+A +VA +++D G++ G P+H A+ + LE+ +LLE + T G+ P+H A+ GH++++ LLL R+ +I G+ +H A+ + + ++L + K ++ + + L +A + V L+ + L + RN Sbjct: 575 GLTPLHVATHYDHQKVALLLLDKGASPHSTAKNGYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRN 739 HSP 11 Score: 72.4034 bits (176), Expect = 5.319e-14 Identity = 38/170 (22.35%), Postives = 78/170 (45.88%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAF 699 G+ +HIA +EV +++++ G+ T G+ P+H A+ G +++ LLL R+ I T G+ +H A+ + ++L + ++ P+H AS G+++++ LL+ + ++ K + L A Q V L+ + + + N A Sbjct: 608 GYTPLHIAAKKNQLEVGKVLLELGAETNMLTQQGVAPLHLAAQEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEASPNELTANGNTAL 777 HSP 12 Score: 55.0694 bits (131), Expect = 1.953e-8 Identity = 30/116 (25.86%), Postives = 56/116 (48.28%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 +EG+AD V L ++ G ++H+A V VA I+ +++ T P+H AS G ++++ LL + +T +G P+H A+ GH+ ++ LL+ + Sbjct: 650 QEGHADMVALLLGRHASISLATKGGLTAMHLAAQEDKVNVAEILTKHKADVNTTTKLCYTPLHVASHYGNIKMVNFLLRNRALVNAKTRNGYTPLHQAAQQGHTHVVNALLQHEAS 765
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000002048.1 (pep scaffold:Pmarinus_7.0:GL477431:30859:73495:1 gene:ENSPMAG00000001850.1 transcript:ENSPMAT00000002048.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 93.2041 bits (230), Expect = 1.124e-20 Identity = 53/188 (28.19%), Postives = 104/188 (55.32%), Query Frame = 1 Query: 91 DFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWG-MRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNN-MSESILELAIQSKQMDCVQLLI 648 D + + +G+ + V K L ++G + +AC G+ E+A++++ +N+E G + P+ A+S G ++I+ LLL D+ ++++G + YA +GG+ +++++LLE N+E + G P+ A+SGGH+++ +LLL+R I+ +N ES L LA ++ V+ L+ Sbjct: 100 DNRSLAEACSDGDVNAVRKLLGEGRSVHETTEEGESLLCLACSAGYYELAQVLLAMHANVEDRGVKGDITPLMAAASGGFVDIVRLLLAHGADVNAQSSTGNTALTYACAGGYVDVVRVLLEAGANLEDHNESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLL 287 HSP 2 Score: 79.7221 bits (195), Expect = 2.847e-16 Identity = 45/165 (27.27%), Postives = 85/165 (51.52%), Query Frame = 1 Query: 166 LNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIE--CETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID-CKNNMSESILELAIQSKQMDCVQLLIS 651 +N ++ ++ +AC GGF+EVA +I G+++E C T P+ A+ G LE++ LL ++ T +G + YA GH+++ LLL+ ++E E G P+ A+ GH+ ++ L+ + ++ N ++L LA + V+LL++ Sbjct: 392 INAQTEETQETALTLACCGGFLEVADFLIKAGADLELGCST-----PLMEAAQEGHLELVKYLLAAGANVHATTATGDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLLA 551 HSP 3 Score: 78.9518 bits (193), Expect = 4.752e-16 Identity = 47/155 (30.32%), Postives = 85/155 (54.84%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETN-SGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCK--NNMSESILELAIQSKQMDCVQLLISV 654 ++ +AC GG ++ ++I G+NIE G P+ A++ G + ++ LLL+ DIE ++ + P+ A SGG E+++LL+ R N E + P+ A+SGG++ I+K+LL I+ + + + S L LA + V+LL+ + Sbjct: 936 ALTLACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDM 1090 HSP 4 Score: 77.0258 bits (188), Expect = 1.746e-15 Identity = 44/151 (29.14%), Postives = 81/151 (53.64%), Query Frame = 1 Query: 208 IACMGGFVEVARIIIDCGSNIECET-TWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMR--PIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +A M G ++++D GS+I + T + A +G E+++LL+ER ++E +G+ P+ A+SGG++E+ ++LLER ++ R + A+ GH + +L++ R ID +N + L LA +D VQLL++ Sbjct: 1074 LAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVN 1224 HSP 5 Score: 74.3294 bits (181), Expect = 1.392e-14 Identity = 41/163 (25.15%), Postives = 84/163 (51.53%), Query Frame = 1 Query: 166 LNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMR--PIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +N ++ + ++ +AC G EV ++++ +N+E G+ P+ A+S G E+ +LLER D+ R + A+ GH + +L++ R +I+ + +G P+ A++GGHL +++LL+ +D +N + L A + + V+ L+ Sbjct: 1094 INAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYLV 1256 HSP 6 Score: 71.633 bits (174), Expect = 1.111e-13 Identity = 45/162 (27.78%), Postives = 83/162 (51.23%), Query Frame = 1 Query: 166 LNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMS-ESILELAIQSKQMDCVQLLI 648 +NT ++ ++ +AC G +E+ R +++ G++ E +T + A G +E+ LLLE + +S P+ A+ GGH E+ LL+ER N+E ++ G P+ A+ GH +++ LLL + I+ + + E+ L LA ++ LI Sbjct: 259 INTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLAQGANINAQTEETQETALTLACCGGFLEVADFLI 420 HSP 7 Score: 68.9366 bits (167), Expect = 7.712e-13 Identity = 44/158 (27.85%), Postives = 80/158 (50.63%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETT-WGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 ++ GH + A GG VEVAR++++ G+ I + + + A +G LE++ LLE D E +T+ + A GH E+ +LLLE + + P+ A+ GGH+++ LL++R ++ N+ + L A + + V LL++ Sbjct: 230 NESGHTPLMEAASGGHVEVARLLLERGAGINTHSNEFKESALTLACYKGHLEMVRFLLEAGADQEHKTDEMHTALMEACMDGHVEVARLLLESGAQVNMPADSFESPLTLAACGGHVELAALLIERGANLEEVNDEGYTPLMEAAREGHEEMVALLLA 387 HSP 8 Score: 63.929 bits (154), Expect = 2.676e-11 Identity = 29/116 (25.00%), Postives = 64/116 (55.17%), Query Frame = 1 Query: 211 ACMGGFVEVARIIIDCGSNIECETTWGMR--PIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 A GG+ EV R++++ G+++ R + A+ +G + L++ R I+ G P+ A++GGH ++++LL+ +++ DNR + P+ A GH+++++ L++ Sbjct: 1142 AASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNKKGNTPLWLAANGGHLDVVQLLVNSGADVDAADNRKITPLMAAFRKGHVKVVRYLVK 1257 HSP 9 Score: 61.2326 bits (147), Expect = 2.009e-10 Identity = 50/198 (25.25%), Postives = 84/198 (42.42%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNS--GMRPIHYASSGGHSEILKLLL----------------------------------ERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMS--ESILELAIQSKQMDCVQLLISVQELFDFINR 681 + +AC GG EV ++I G+N E P+ A+S G + I+ +LL +I T S G+ P+ A+ GH+ +KLLL ER N+E G+ P+ A+SGG+ ++ ++LL+R ++ S ++ L +A +L+IS D N+ Sbjct: 1004 LSLACSGGRQEVVELLIARGANKEHRNVSDYTPLSLAASGGYVNIIKILLNAGAEINSRTGSKLGISPLMLAAMNGHTAAVKLLLDMGSDINAQIETNRNTALTLACFQGRHEVVSLLVERKANVEHRAKTGLTPLMEAASGGYAEVGRVLLERGADVNAAPVPSSRDTALTIAADKGHYKFCELVISRGAHIDVRNK 1201 HSP 10 Score: 56.225 bits (134), Expect = 9.175e-9 Identity = 31/100 (31.00%), Postives = 58/100 (58.00%), Query Frame = 1 Query: 364 DIEC---ETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMS-ESILELAIQSKQMDCVQLLIS 651 DI+C E+N + A +GGH +++ +L+ R NIE D +G P+ A++ GH+ +++LLL I+ ++ + ++ L LA + + V+LLI+ Sbjct: 923 DIDCIHTESNHDT-ALTLACAGGHEDLVTVLIARGANIEHRDKKGFTPLILAATAGHVNVVELLLDNGADIEAQSERTKDTPLSLACSGGRQEVVELLIA 1021 HSP 11 Score: 47.7506 bits (112), Expect = 4.030e-6 Identity = 30/121 (24.79%), Postives = 59/121 (48.76%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECET-NSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLL 549 G ++ AC G +VA +++ G+++E E+ G P+ A+ G + + L+ + D+ T N+ + A +GGH +++LLL + G + A+ GGH ++ L+ Sbjct: 463 GDTALTYACENGHTDVADLLLQAGADLEHESEGGRTPLMKAARAGHVCTVQFLISKGADVNRATANNDHTVLSLACAGGHLSVVELLLAHGADPNHRLKDGSTMLIEAAKGGHTSVVCYLI 583
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007449.1 (pep scaffold:Pmarinus_7.0:GL478728:201453:233342:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007449.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 89.7373 bits (221), Expect = 1.261e-19 Identity = 48/175 (27.43%), Postives = 83/175 (47.43%), Query Frame = 1 Query: 124 GNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 GN + V L+ + +G ++H+A G EV R ++ G+ ++ + P+H A+ G LEI+ LLL+ + T++G P+H A+ GH ++ +LLE ++ +G P+H AS G +++ LLLQR D + L +A LL+ Sbjct: 59 GNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLL 233 HSP 2 Score: 81.6481 bits (200), Expect = 5.979e-17 Identity = 46/185 (24.86%), Postives = 81/185 (43.78%), Query Frame = 1 Query: 97 QCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 Q LQH+ +A G+ +H+A G +V I+++ G+++ T G P+H AS G +E+ +LLL+R + +G+ P+H AS + ++ LLL++ + G P+H A+ L+I LL+ + + L L Q D LL++ Sbjct: 131 QLLLQHMASPDA---------------ATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLA 300 HSP 3 Score: 78.1814 bits (191), Expect = 7.238e-16 Identity = 40/153 (26.14%), Postives = 74/153 (48.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++IE T G+ P+H ++ G L I+ +LL+ G +H A+ G +E+++ LL ++ + P+H A+ G L+I++LLLQ D + + L +A + D +L+ Sbjct: 15 GFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILL 167 HSP 4 Score: 74.3294 bits (181), Expect = 1.229e-14 Identity = 38/153 (24.84%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A +++ G+ T G+ P+H + G ++ +LLL R D+ T SG+ P+H A+ + ++L++ ++ G P+ A G+++++ LLQ ++ K + L A Q V LL+ Sbjct: 246 GYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 398 HSP 5 Score: 74.3294 bits (181), Expect = 1.309e-14 Identity = 36/134 (26.87%), Postives = 72/134 (53.73%), Query Frame = 1 Query: 247 IIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++D G+N G P+H A + + ++ LLL+ IE T SG+ P+H ++ G+ I+ +LL+ + + RG +H A+ G ++++ LL+ ++D K ++ L +A + +++ VQLL+ Sbjct: 1 LLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL 134 HSP 6 Score: 74.3294 bits (181), Expect = 1.524e-14 Identity = 43/177 (24.29%), Postives = 79/177 (44.63%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +EG+ D L+ + +G +H+A G +EVA +++ ++ + G+ P+H AS ++ LLL++ +G P+H A+ EI LLE +G+ P+H + GH + LLL R ++ S L LA Q +++ ++L+ Sbjct: 156 REGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 332 HSP 7 Score: 73.9442 bits (180), Expect = 1.992e-14 Identity = 40/161 (24.84%), Postives = 75/161 (46.58%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 +HIA G +E+ ++++ ++ + T+ G P+H A+ G ++ +LLE + T G P+H AS G E+ LLL+R + + G+ P+H AS + ++ LLL + + L +A + Q++ L+ + I R Sbjct: 118 LHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQ 278 HSP 8 Score: 72.7886 bits (177), Expect = 4.410e-14 Identity = 37/153 (24.18%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H + GH+++ LLL R ++ G+ P+H A+ + + ++L++ ++ + + L +A + V L+ Sbjct: 213 GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL 365 HSP 9 Score: 68.1662 bits (165), Expect = 1.263e-12 Identity = 35/157 (22.29%), Postives = 72/157 (45.86%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G +H++ G + + I++ G++ G +H A+ G E++ LL ++ + P+H A+ G EI++LLL+ + + + G P+H A+ GH + +LL+ + + L +A + +M+ LL+ Sbjct: 44 VTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL 200
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007446.1 (pep scaffold:Pmarinus_7.0:GL478728:200023:240674:1 gene:ENSPMAG00000006704.1 transcript:ENSPMAT00000007446.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 82.8037 bits (203), Expect = 2.255e-17 Identity = 39/123 (31.71%), Postives = 68/123 (55.28%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQR 555 +G ++H+A G EV R ++ G+ ++ + P+H A+ G LEI+ LLL+ + T++G P+H A+ GH ++ +LLE ++ +G P+H AS G +++ LLLQR Sbjct: 170 RGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQR 292 HSP 2 Score: 80.4925 bits (197), Expect = 1.262e-16 Identity = 40/150 (26.67%), Postives = 81/150 (54.00%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G A++++D G+N G P+H A + + ++ LLL+ IE T SG+ P+H ++ G+ I+ +LL+ + + RG +H A+ G ++++ LL+ ++D K ++ L +A + +++ VQLL+ Sbjct: 75 ALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLL 224 HSP 3 Score: 73.9442 bits (180), Expect = 1.687e-14 Identity = 41/154 (26.62%), Postives = 74/154 (48.05%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G+ +H+A G +V I+++ G+++ T G P+H AS G +E+ +LLL+R + +G+ P+H AS + ++ LLL++ + G P+H A+ L+I LL+ + + L L Q D LL++ Sbjct: 237 GYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLA 390 HSP 4 Score: 73.9442 bits (180), Expect = 1.947e-14 Identity = 40/153 (26.14%), Postives = 74/153 (48.37%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + V +++ G++IE T G+ P+H ++ G L I+ +LL+ G +H A+ G +E+++ LL ++ + P+H A+ G L+I++LLLQ D + + L +A + D +L+ Sbjct: 105 GFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILL 257 HSP 5 Score: 70.4774 bits (171), Expect = 2.624e-13 Identity = 38/153 (24.84%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +E+A +++ G+ T G+ P+H + G ++ +LLL R D+ T SG+ P+H A+ + ++L++ ++ G P+ A G+++++ LLQ ++ K + L A Q V LL+ Sbjct: 336 GYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLLQHGADVNSKTKNGYTPLHQAAQQGHTHIVNLLL 488 HSP 6 Score: 68.1662 bits (165), Expect = 1.274e-12 Identity = 37/153 (24.18%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + LEI T LLE + T G+ P+H + GH+++ LLL R ++ G+ P+H A+ + + ++L++ ++ + + L +A + V L+ Sbjct: 303 GLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILVKHGAHVNATTKLGYTPLIVACHYGNIKMVNFLL 455 HSP 7 Score: 67.3958 bits (163), Expect = 2.753e-12 Identity = 43/177 (24.29%), Postives = 79/177 (44.63%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +EG+ D L+ + +G +H+A G +EVA +++ ++ + G+ P+H AS ++ LLL++ +G P+H A+ EI LLE +G+ P+H + GH + LLL R ++ S L LA Q +++ ++L+ Sbjct: 246 REGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLLEYGAETNAITRQGVAPLHLGAQEGHADVASLLLARYADVNHATKSGLSPLHLAAQEDKVNVAEILV 422 HSP 8 Score: 67.0106 bits (162), Expect = 3.345e-12 Identity = 38/149 (25.50%), Postives = 72/149 (48.32%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G +E+ ++++ ++ + T+ G P+H A+ G ++ +LLE + T G P+H AS G E+ LLL+R + + G+ P+H AS + ++ LLL + + L +A + Q++ L+ Sbjct: 208 LHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLLQRFASPDAAGKNGLTPLHVASHYDNQKVALLLLDQGASPHATAKNGYTPLHIAAKKDQLEIATTLL 356 HSP 9 Score: 61.6178 bits (148), Expect = 1.900e-10 Identity = 35/157 (22.29%), Postives = 72/157 (45.86%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G +H++ G + + I++ G++ G +H A+ G E++ LL ++ + P+H A+ G EI++LLL+ + + + G P+H A+ GH + +LL+ + + L +A + +M+ LL+ Sbjct: 134 VTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLLRNGAQVDAKAREEQTPLHIAARLGKLEIVQLLLQHMASPDAATSNGYTPLHVAAREGHEDVTAILLEHGASLAVTTKKGFTPLHVASKYGKMEVASLLL 290 HSP 10 Score: 60.8474 bits (146), Expect = 2.724e-10 Identity = 37/153 (24.18%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G VE ++++ + ++ T + +H A+ G +LL++ + +G P+H A +++LLL+ +IE G+ P+H ++ G+L I+ +LLQ + N E+ L +A ++ Q + V+ L+ Sbjct: 39 GLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLLKYGASIEAVTESGLTPMHVSAFMGNLNIVLILLQGGASPNATNVRGETALHMAARAGQTEVVRSLL 191 HSP 11 Score: 56.6102 bits (135), Expect = 5.558e-9 Identity = 29/120 (24.17%), Postives = 65/120 (54.17%), Query Frame = 1 Query: 289 GMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ P+H A+ G +++ +LLE+ I +T +G+ P+H A+ G H E +++L++ ++ + +H A+ GH + K+LL + + + + L +A + ++ ++LL+ Sbjct: 6 GLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPVDDITLDYLTALHVAAHCGHYRTAKVLLDKGANPNVRALNGFTPLHIACKKNRIRVMELLL 125 HSP 12 Score: 51.6026 bits (122), Expect = 2.450e-7 Identity = 33/110 (30.00%), Postives = 52/110 (47.27%), Query Frame = 1 Query: 487 GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 G+ P+HCA+ GH Q++++LL++ I K S L +A Q ++CVQ+L+ + D+I D + + LHV A C R K +LDK Sbjct: 6 GLTPLHCAARSGHEQVVEILLEQGAPILAKTKNGLSPLHMATQGDHVECVQVLMQYRAPV------DDITLDYL------------TALHV---AAHCGHYRTAKVLLDK 94
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Yeast
Match: NAS6 (Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6 [Source:SGD;Acc:S000003464]) HSP 1 Score: 52.373 bits (124), Expect = 2.407e-8 Identity = 34/118 (28.81%), Postives = 65/118 (55.08%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLL--LERNCDIECETNSGMRPIHYASSGGHSEILKLLLER-NCNIECEDNRGMR 495 LK D LN I + QG +H+A + EV++ +I+ G+++ + + P+H A+S G L+++ LL L ++ + + G P+ +A + GH + LL+E+ + DN+G + Sbjct: 96 LKPD-LNKITN-QGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSA-VNWQDKQGWTPLFHALAEGHGDAAVLLVEKYGAEYDLVDNKGAK 210 HSP 2 Score: 51.6026 bits (122), Expect = 3.757e-8 Identity = 35/134 (26.12%), Postives = 64/134 (47.76%), Query Frame = 1 Query: 166 LNTIVDDQGHYSIHIACMGGFVEVARIIID--CGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLL--LERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQR 555 L+ DD G HIAC G +EV + + D ++ T G+ +H A + E+ L+E + + P+H A+S G ++++LL L ++ + +D +G P+ A + GH LL+++ Sbjct: 64 LDDYPDDSGWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSA-VNWQDKQGWTPLFHALAEGHGDAAVLLVEK 196 HSP 3 Score: 49.2914 bits (116), Expect = 2.578e-7 Identity = 34/123 (27.64%), Postives = 59/123 (47.97%), Query Frame = 1 Query: 289 GMRPIHYASSRGCLEILTLLLER--NCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ-RKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G P H A S G LE++ L +R D+ TN G+ +H A E+ + L+E ++ +D P+H A+S G L++++LL K ++ ++ + L A+ D LL+ Sbjct: 72 GWTPFHIACSVGNLEVVKSLYDRPLKPDLNKITNQGVTCLHLAVGKKWFEVSQFLIENGASVRIKDKFNQIPLHRAASVGSLKLIELLCGLGKSAVNWQDKQGWTPLFHALAEGHGDAAVLLV 194
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Yeast
Match: AKR2 (Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication [Source:SGD;Acc:S000005560]) HSP 1 Score: 50.447 bits (119), Expect = 3.529e-7 Identity = 39/176 (22.16%), Postives = 70/176 (39.77%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNI-ECETTWGMRPIHYASSRGCLEILTLLLER---------------------------------------NCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELA 609 +H AC+ VA+ ++ G+N + G +H+A+ G + I+ LLL+ N DI+ + N+ P+ +A+ G ++LLL+ + DNRG +HCA GG +++ L+ +NN + +LA Sbjct: 88 LHWACINNRFSVAKFLLLRGANPNQAAGPGGATALHWAARYGNIYIVDLLLKHGADPTLKDEQGLNIMHFSVYSSNILLVVYVLYFVVNNNDNVDIDSKDNNNRTPLLWAAYQGDFLTVELLLKFGSTVAWTDNRGFNALHCALVGGDQRVICDLILSGANFYERNNQKQDCFDLA 263
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Yeast
Match: PHO81 (Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress [Source:SGD;Acc:S000003465]) HSP 1 Score: 47.3654 bits (111), Expect = 3.077e-6 Identity = 22/53 (41.51%), Postives = 31/53 (58.49%), Query Frame = 1 Query: 397 PIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQR 555 PI YA GHSE++ LL+ N ++ ED+ G P+ A H+ +L LLQR Sbjct: 595 PIFYAVRSGHSEVITELLKHNARLDIEDDNGHSPLFYALWESHVDVLNALLQR 647
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Yeast
Match: HOS4 (Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate [Source:SGD;Acc:S000001374]) HSP 1 Score: 46.595 bits (109), Expect = 5.287e-6 Identity = 36/121 (29.75%), Postives = 59/121 (48.76%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERN-CDIECETNSGMRPIHYASSGGHSEILKLLLER--NCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 S HIA + I D G G + A +G +++ ++E DI + N+G +H A+ GH EI++LL+E + NI+ + G P+ AS+ GHL ++K LL+ Sbjct: 309 SSHIAVSKRPKQKKGIYRDSG---------GRTRLQIACDKGKYDVVKKMIEEGGYDINDQDNAGNTALHEAALQGHIEIVELLIENGADVNIKSIEMFGDTPLIDASANGHLDVVKYLLK 420
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Match: EDO43690 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXV8]) HSP 1 Score: 71.2478 bits (173), Expect = 6.093e-14 Identity = 56/227 (24.67%), Postives = 95/227 (41.85%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEIL--------------------------KLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFD 702 FL + G+ D V K L + G ++H AC+ ++ ++++D G++++ G +H A+S G +EI L+E D+ N G P+ A ++L + L +NC E +D +H ASS G+L+++ LLLQ I+ K+ + + L A Q D +LL + DF RN + D Sbjct: 52 VFLSAVSSGDVDEVKKLLAKGADINYQNVDGLTALHQACIDENFDLVKVLVDKGASMDVRDNEGWTSLHAAASCGSVEIAKYLIENGADVAAVNNEGELPLDLAEEEDMEDLLTDEIENQGIDVDDARAVEEKIMLNDAQSWLNKNCINESKDKTEASALHVASSKGYLKVIGLLLQLGVEINAKDADNWTPLHAAAHWGQKDACELL--SENGADFNARNKMVGID 276
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Match: EDO46099 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RR35]) HSP 1 Score: 64.3142 bits (155), Expect = 1.674e-12 Identity = 43/136 (31.62%), Postives = 67/136 (49.26%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMR---PIHYASSRGCLE-ILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECED-NRGMRPIHCASSGGHLQILKL 543 LKS + G IH A M G E R+++ GS+ +T +R P+HYA+ G L+ + +L+ER D+E G R IH AS G ++L LL+ +IE + R + +H A+ G + + L Sbjct: 17 LKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMERGIDLEAVYRDGWRLIHVASDNGRVKVLVTLLDAGVDIESKTYERRLTAVHVAARQGQVTMTTL 152 HSP 2 Score: 52.373 bits (124), Expect = 2.161e-8 Identity = 28/89 (31.46%), Postives = 48/89 (53.93%), Query Frame = 1 Query: 301 IHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDN---RGMRPIHCASSGGHLQ-ILKLLLQR 555 +HYA+ G + + LLL+ + G RPIH A+ GH+E +++LL + + R M P+H A+ GHL +++L++R Sbjct: 1 LHYAARYGNEKCIELLLKSGAKLNVPQRYGWRPIHEAAMNGHTECVRMLLRYGSHANAKTGKTIRNMTPLHYAARMGHLDCAVRMLMER 89
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Match: EDO34161 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SQ63]) HSP 1 Score: 62.3882 bits (150), Expect = 2.351e-12 Identity = 28/73 (38.36%), Postives = 46/73 (63.01%), Query Frame = 1 Query: 232 EVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLL 450 EV + I G++ + G P+H A +RG LE++ +L++ D+ +N G+RP+H A+ GGH E+L+LLL Sbjct: 1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLVQYGADVNAHSNDGIRPLHDAAEGGHVEVLRLLL 73 HSP 2 Score: 58.5362 bits (140), Expect = 5.054e-11 Identity = 24/73 (32.88%), Postives = 46/73 (63.01%), Query Frame = 1 Query: 331 EILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLL 549 E++ + + D + N+G P+H A + G E++K+L++ ++ N G+RP+H A+ GGH+++L+LLL Sbjct: 1 EVVKQCILQGADPTAKDNAGWTPLHEACARGKLEVVKVLVQYGADVNAHSNDGIRPLHDAAEGGHVEVLRLLL 73 HSP 3 Score: 48.9062 bits (115), Expect = 1.266e-7 Identity = 24/73 (32.88%), Postives = 41/73 (56.16%), Query Frame = 1 Query: 172 TIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSG 390 T D+ G +H AC G +EV ++++ G+++ + G+RP+H A+ G +E+L LLL D T +G Sbjct: 14 TAKDNAGWTPLHEACARGKLEVVKVLVQYGADVNAHSNDGIRPLHDAAEGGHVEVLRLLLTYGADPMLATYAG 86
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Match: EDO38702 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SC57]) HSP 1 Score: 66.6254 bits (161), Expect = 5.074e-12 Identity = 42/153 (27.45%), Postives = 80/153 (52.29%), Query Frame = 1 Query: 238 ARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERN-CDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQE---LFDFINRN 684 +++++D G+ I+ + + PIHYA+ G + + LL+R+ I E + P+H A+ GH+ ++K LL R I+ + P+ CA++ G +++ +LL +D + + L LA + ++ V+LL+S + L D RN Sbjct: 266 SQLLLDNGACIDSKNDEDITPIHYAAKFGRVRTVCALLKRDPAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRN 418 HSP 2 Score: 63.5438 bits (153), Expect = 5.174e-11 Identity = 40/142 (28.17%), Postives = 68/142 (47.89%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYS---IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQI 534 K G V LK D I++D+ S +H+A + G V + ++ G I+ P+ A++RG +E+ +LL+ + ++ + P+H AS GH E++KLLL R NI D+ G + A H ++ Sbjct: 292 KFGRVRTVCALLKRD--PAIINDEDGASNTPLHLAALHGHAAVVKELLYRGGAIDARNATLWTPLDCAAARGMVEVANILLDEDAPVDPTDKAKTTPLHLASREGHVEMVKLLLSRKANITLTDSAGRNCLDIAIESSHKEV 431 HSP 3 Score: 49.6766 bits (117), Expect = 1.357e-6 Identity = 56/228 (24.56%), Postives = 98/228 (42.98%), Query Frame = 1 Query: 292 MRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLL----------ERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDC-KNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMA-IECNADRIVKCILDKTVDDEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQ 939 M P+H A S GC+ + L+E D+ M P+H+A G+ + KLL + + +D +H A GGH + KL L++ ++ K + +++ + + +++ C+ ++I V +L + KS RE + L+ D+ I C R CI + D KDT +EKN D +R + L++ DIA + Sbjct: 5 MTPLHVACSHGCIPVAKCLVEAGADLRSLDEEQMTPLHFACMEGNLGVAKLLFVAAELRGGWSTVSKMVTDQDREEQTALHLAVEGGHGDLAKLCLEKGANVNAVKESKNKTFHKTIVVARE--CIFVMIKVSKL----------KYLSFCKSEKIKREIL---LYFFDVVIIICYIIRFRACIECRDKD----KDTPLMIAAEKNHVDTVRTL----LQHGADIAAK 209
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Match: EDO28178 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T774]) HSP 1 Score: 64.3142 bits (155), Expect = 5.341e-12 Identity = 42/136 (30.88%), Postives = 73/136 (53.68%), Query Frame = 1 Query: 217 MGGFVEVARIIIDCGSNIECETTWGM-RPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC--NIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQ 615 + G V+VA +++D G+ I E+ P+ +A +G +++ LLL R+ +IE T G P+ +A+ GGH+++ LLE+ NI N + P+ A GH ++KLLL+ I+ + + L LA + Sbjct: 62 LAGHVKVATMLLDHGAEINVESDSNKDSPLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDI-PLTSACWKGHHDVVKLLLKYTSNIEHRTKDGCTPLMLAAR 196 HSP 2 Score: 56.225 bits (134), Expect = 3.171e-9 Identity = 33/111 (29.73%), Postives = 60/111 (54.05%), Query Frame = 1 Query: 322 GCLEILTLLLERNCDIECETNSGM-RPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESI-LELAIQSKQMDCVQLLI 648 G +++ T+LL+ +I E++S P+ +A GH ++++LLL R+ NIE G P+ A+ GGH + LL++ +++ + + I L A D V+LL+ Sbjct: 64 GHVKVATMLLDHGAEINVESDSNKDSPLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACWKGHHDVVKLLL 174 HSP 3 Score: 50.447 bits (119), Expect = 2.757e-7 Identity = 32/117 (27.35%), Postives = 54/117 (46.15%), Query Frame = 1 Query: 166 LNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMR-PIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCAS 513 +N D + AC G +V +++ +NIE T G P+ +A+ G ++ LLE+ + + S P+ A GH +++KLLL+ NIE G P+ A+ Sbjct: 79 INVESDSNKDSPLTFACWKGHCDVVELLLARSANIEHRTKEGFTPLMFAALGGHTDVAAKLLEQGAKVNIPSGSNNDIPLTSACWKGHHDVVKLLLKYTSNIEHRTKDGCTPLMLAA 195
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Match: ENSORLT00000040233.1 (pep primary_assembly:ASM223467v1:19:16412534:16434057:1 gene:ENSORLG00000021910.1 transcript:ENSORLT00000040233.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 108.997 bits (271), Expect = 6.994e-26 Identity = 61/209 (29.19%), Postives = 108/209 (51.67%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + + P+H AS G+S +++LLLER I+ G+ P+HCA+ GH Q++++LL R I K S L +A Q ++CVQLL+ + ++ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 181 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLL-----------HHDVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKVIVDKKAN 368 HSP 2 Score: 92.8189 bits (229), Expect = 2.973e-20 Identity = 44/140 (31.43%), Postives = 77/140 (55.00%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G + VA ++++ G+ ++ + + P+H AS RG ++ LLLER I+ T G+ P+H A+ GH +++++LL R I + G+ P+H A+ G HL ++LLL +D Sbjct: 198 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRGAAVDFKARNDITPLHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHDVPVD 337 HSP 3 Score: 78.1814 bits (191), Expect = 2.269e-15 Identity = 39/149 (26.17%), Postives = 82/149 (55.03%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G + R++++ GS I+ T G+ P+H A+ G +++ +LL R I +T +G+ P+H A+ G H ++LLL + ++ N + +H A+ GH ++ K+++ +K + K + L +A + ++ ++LL+ Sbjct: 248 LHVASKRGNSNMVRLLLERGSKIDARTKDGLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLL 396 HSP 4 Score: 77.7962 bits (190), Expect = 2.955e-15 Identity = 46/177 (25.99%), Postives = 83/177 (46.89%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 + GN + YL++ I + G ++H+A G VEV +I G+N++ T G +H AS G E++ L+ ++ ++ +G P++ A+ H ++++LLLE + G P+ A GH Q++ LLL+ D K + L +A + LL+ Sbjct: 26 RAGNLEKALDYLQNGVDINICNQNGLNALHLASKEGHVEVVAELIKQGANVDAATKKGNTALHIASLAGQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLENGSSQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLL 198 HSP 5 Score: 68.1662 bits (165), Expect = 4.266e-12 Identity = 37/146 (25.34%), Postives = 69/146 (47.26%), Query Frame = 1 Query: 211 ACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 A G +E A + G +I G+ +H AS G +E++ L+++ +++ T G +H AS G +E++K L+ N+ + G P++ A+ HL +++LLL+ + L +A+Q V LL+ Sbjct: 24 AARAGNLEKALDYLQNGVDINICNQNGLNALHLASKEGHVEVVAELIKQGANVDAATKKGNTALHIASLAGQTEVVKELVSNGANVNAQSQNGFTPLYMAAQENHLDVVQLLLENGSSQSIATEDGFTPLAVALQQGHDQVVSLLL 169 HSP 6 Score: 62.3882 bits (150), Expect = 3.005e-10 Identity = 32/128 (25.00%), Postives = 65/128 (50.78%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDC 573 G +H A G +V ++++ G+ I +T G+ P+H A+ L + LLL + ++ TN + +H A+ GH ++ K+++++ N + G P+H A +++++LLL+ I Sbjct: 277 GLTPLHCAARSGHEQVVEMLLNRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLHHDVPVDDVTNDYLTALHVAAHCGHYKVAKVIVDKKANPNAKALNGFTPLHIACKKNRVKVMELLLKHGASIQA 404
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Match: ENSORLT00000039903.1 (pep primary_assembly:ASM223467v1:15:18469388:18484014:-1 gene:ENSORLG00000030042.1 transcript:ENSORLT00000039903.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 104.375 bits (259), Expect = 2.930e-24 Identity = 60/209 (28.71%), Postives = 106/209 (50.72%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + E+ G P+H A+ G + + TLLL R ++ + + P+H A+ G+S ++KLLL+R IE + G+ P+HC + GH Q++++LL R I K S L +A Q ++CVQLL+ ++ D + ++D++ + LHV A C ++ K I+DK + Sbjct: 181 ALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQ-----------HDVPVDDV--TNDYL-----TALHV---AAHCGHYKVAKLIVDKKAN 368 HSP 2 Score: 90.1225 bits (222), Expect = 2.117e-19 Identity = 46/166 (27.71%), Postives = 86/166 (51.81%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 L++D + G +HIA G + VA ++++ + ++ + P+H A+ RG ++ LLL+R IE +T G+ P+H + GH +++++LL+R I + G+ P+H A+ G HL ++LLLQ +D N + L +A +L++ Sbjct: 198 LQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIV 363 HSP 3 Score: 84.7297 bits (208), Expect = 1.278e-17 Identity = 62/254 (24.41%), Postives = 112/254 (44.09%), Query Frame = 1 Query: 100 CFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFINRNDEIAFDKI---------WKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 +L+ + GN + V YLKS I + G ++H+A G VEV ++ G++++ T G +H +S G E++T L+ ++ ++ +G P++ A+ H E+++ LLE + + G P+ A GH Q++ LLL+ D K + L +A + LL+ V+ F + + I + DF+ N + LHV A + +VK +LD+ Sbjct: 20 SYLRAARAGNLEKVLDYLKSGVEINICNQNGLNALHLASKEGHVEVVAELLKLGASVDAATKKGNTALHISSLAGQAEVVTELVTNGANVNAQSQNGFTPLYMAAQENHLEVVRFLLENSASQSIATEDGFTPLAVALQQGHDQVVSLLLEN----DTKGKVRLPALHIAARKDDTKAAALLLQNDHNADVESKSGFTPLHIAAHYGNINVATLLLNRAAAVDFMARNDITPLHV---AAKRGNSNMVKLLLDR 266 HSP 4 Score: 82.0333 bits (201), Expect = 1.009e-16 Identity = 51/205 (24.88%), Postives = 103/205 (50.24%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI----SVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKC 804 +H+A G + ++++D G+ IE +T G+ P+H + G +++ +LL+R I +T +G+ P+H A+ G H ++LLL+ + ++ N + +H A+ GH ++ KL++ +K + K + L +A + ++ ++LL+ S+Q + E I ++ ENI S L ++ N +V C Sbjct: 248 LHVAAKRGNSNMVKLLLDRGARIEAKTKDGLTPLHCGARSGHEQVVEILLDRGAPILSKTKNGLSPLHMATQGDHLNCVQLLLQHDVPVDDVTNDYLTALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKHGASIQAV-------TESGLTPIHVAAFMGHENIVSAL--INHGASPNTTNVVSC 443 HSP 5 Score: 59.6918 bits (143), Expect = 1.888e-9 Identity = 27/89 (30.34%), Postives = 48/89 (53.93%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCN 465 ++H+A G +VA++I+D +N + G P+H A + + ++ LLL+ I+ T SG+ PIH A+ GH I+ L+ + Sbjct: 346 ALHVAAHCGHYKVAKLIVDKKANPNAKALNGFTPLHIACKKNRVRVMELLLKHGASIQAVTESGLTPIHVAAFMGHENIVSALINHGAS 434
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Match: ENSORLT00000006308.2 (pep primary_assembly:ASM223467v1:1:12516834:12530692:1 gene:ENSORLG00000005003.2 transcript:ENSORLT00000006308.2 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 101.293 bits (251), Expect = 2.982e-23 Identity = 58/209 (27.75%), Postives = 103/209 (49.28%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVD 825 ++HIA + A +++ N + ++ G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ ++ LLL+R I+ + G+ P+HCA+ GH ++LLL+R I + S L ++ Q ++CV+LL+ Q D++ D + + LHV A C R+ K +LDK + Sbjct: 151 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTNMVALLLDRGAQIDAKTRDGLTPLHCAARSGHDPAVELLLERGAPILARTKNGLSPLHMSAQGDHIECVKLLLQHQAPV------DDVTLDYL------------TALHV---AAHCGHYRVTKVLLDKKAN 338 HSP 2 Score: 98.2117 bits (243), Expect = 3.124e-22 Identity = 46/140 (32.86%), Postives = 79/140 (56.43%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G V V+ ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH ++LLLER I G+ P+H ++ G H++ +KLLLQ + +D Sbjct: 168 LQNDHNADVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTNMVALLLDRGAQIDAKTRDGLTPLHCAARSGHDPAVELLLERGAPILARTKNGLSPLHMSAQGDHIECVKLLLQHQAPVD 307 HSP 3 Score: 77.7962 bits (190), Expect = 2.291e-15 Identity = 42/153 (27.45%), Postives = 81/153 (52.94%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A G + +++D G+ I+ +T G+ P+H A+ G + LLLER I T +G+ P+H ++ G H E +KLLL+ ++ + +H A+ GH ++ K+LL +K + + + L +A + ++ ++LL+ Sbjct: 214 GITPLHVASKRGNTNMVALLLDRGAQIDAKTRDGLTPLHCAARSGHDPAVELLLERGAPILARTKNGLSPLHMSAQGDHIECVKLLLQHQAPVDDVTLDYLTALHVAAHCGHYRVTKVLLDKKANPNARALNGFTPLHIACKKNRVKVMELLV 366 HSP 4 Score: 73.9442 bits (180), Expect = 4.918e-14 Identity = 47/206 (22.82%), Postives = 88/206 (42.72%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC-----------------------------NIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 KEG+ D V + L+ +G+ ++HIA + G EV ++++ G+++ ++ G P++ A+ LE++ LE + T G P+ A GH+ ++ LLLE + N + + G P+H A+ G++ + LLL R +D + L +A + + V LL+ Sbjct: 29 KEGHKDLVEELLQRGAPVDSATKKGNTALHIASLAGQKEVVKLLVSRGADVNAQSQNGFTPLYMAAQENHLEVVRYFLENEGNQSIATEDGFTPLAIALQQGHNSVVSLLLEHDTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTNMVALLL 234 HSP 5 Score: 70.8626 bits (172), Expect = 4.380e-13 Identity = 56/243 (23.05%), Postives = 101/243 (41.56%), Query Frame = 1 Query: 133 DGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS------VQELFDFINRNDEIAFDKIWKSS---------DFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 D V ++LK+ + + G ++H+A G ++ ++ G+ ++ T G +H AS G E++ LL+ R D+ ++ +G P++ A+ H E+++ LE N G P+ A GH ++ LLL+ D K + L +A + LL+ VQ F + + + S+ DF N + LHV N +V +LD+ Sbjct: 1 DKVLEFLKNGVDISTCNQNGLNALHLAAKEGHKDLVEELLQRGAPVDSATKKGNTALHIASLAGQKEVVKLLVSRGADVNAQSQNGFTPLYMAAQENHLEVVRYFLENEGNQSIATEDGFTPLAIALQQGHNSVVSLLLEH----DTKGKVRLPALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVSTLLLNRGAAVDFTARNGITPLHVASKRGNTN---MVALLLDR 236 HSP 6 Score: 57.3806 bits (137), Expect = 1.213e-8 Identity = 26/94 (27.66%), Postives = 50/94 (53.19%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECED 480 ++H+A G V ++++D +N G P+H A + ++++ LL++ I+ T SG+ PIH A+ GH I+ LLL+ + + + Sbjct: 316 ALHVAAHCGHYRVTKVLLDKKANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVRN 409
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Match: ank1b (ankyrin-1 [Source:NCBI gene;Acc:101157380]) HSP 1 Score: 94.3597 bits (233), Expect = 2.414e-20 Identity = 56/206 (27.18%), Postives = 98/206 (47.57%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA A +++ N + + G P+H A+ + + LLL R ++ +G+ P+H AS G+ +++LLL+R I+ + + P+HCA+ GH++I+++LL+ I K S + +A Q MDC V++L + D+I D + + LHV A C R+ K +LDK Sbjct: 174 ALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDC------VRQLLQYNAEIDDITLDHL------------TPLHV---AAHCGHHRMAKVLLDK 358 HSP 2 Score: 91.2781 bits (225), Expect = 2.516e-19 Identity = 45/140 (32.14%), Postives = 79/140 (56.43%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D ++ G +HIA + VA+++++ G+N+ G+ P+H AS RG + ++ LLL+R I+ +T + P+H A+ GH I+++LLE I+ + G+ PIH A+ G H+ ++ LLQ ID Sbjct: 191 LQNDPNPDVLSKTGFTPLHIAAHYENMSVAQLLLNRGANVNFTPKNGITPLHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEID 330 HSP 3 Score: 90.8929 bits (224), Expect = 3.130e-19 Identity = 45/165 (27.27%), Postives = 86/165 (52.12%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 G+ +HIA +EVA +++ G++ CE+ G+ P+H AS G +++ +L+ + ++ +G+ P+H + GH I L+++ ++ G P+H A G+++++K LLQ++ ++ K M + L A Q D V LL+ L + I N Sbjct: 600 GYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSN 764 HSP 4 Score: 88.9669 bits (219), Expect = 1.455e-18 Identity = 49/165 (29.70%), Postives = 83/165 (50.30%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRN 684 GH +HIA G + RI++D G+ T G P+H AS G +++ LLLER + +G+ P+H A + +++KLL+ + + G P+H A+ +++ +LLQ +C++ + L LA Q + D V +LIS Q + N+N Sbjct: 501 GHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKN 665 HSP 5 Score: 84.7297 bits (208), Expect = 3.501e-17 Identity = 44/149 (29.53%), Postives = 83/149 (55.70%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G V + R+++D G+ I+ +T + P+H A+ G + I+ +LLE I+ +T +G+ PIH A+ G H + ++ LL+ N I+ + P+H A+ GH ++ K+LL + + + + + L +A + M + LL+ Sbjct: 241 LHIASRRGNVMMVRLLLDRGAQIDAQTKDELTPLHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLL 389 HSP 6 Score: 83.9593 bits (206), Expect = 6.413e-17 Identity = 42/149 (28.19%), Postives = 78/149 (52.35%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +H+A G +A++++D G+ G P+H A + + + LLL+ + +E T SG+ P+H A+ GH I+K LL+R + + + P+H AS GH ++ + LLQ ++D + ++ L A + + V+LL+ Sbjct: 340 LHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLL 488 HSP 7 Score: 80.1073 bits (196), Expect = 9.073e-16 Identity = 41/153 (26.80%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC + +++ +++E T G+ P+H A+ G L I+ LL+R P+H AS GH E+ + LL+ ++ P+HCA+ GH +++KLLL K D + L +A + + +++L+ Sbjct: 369 GFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILL 521 HSP 8 Score: 77.411 bits (189), Expect = 7.152e-15 Identity = 40/150 (26.67%), Postives = 75/150 (50.00%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 +H+A G EVA+ ++ + ++ P+H A+ G E++ LLL+ + + T +G P+H A+ GH +++LL+ +G P+H AS G + + +LLL+R + + L +A+ +D V+LL+S Sbjct: 439 LHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVS 588 HSP 9 Score: 74.3294 bits (181), Expect = 7.516e-14 Identity = 50/196 (25.51%), Postives = 89/196 (45.41%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC-------------NIECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDE 690 +H A G V + I+++ G+ I+ +T G+ PIH A+ ++ + LL+ N +I+ T + P+H A+ GH + K+LL++ +I C+ N G+ P+H A+ GHL I+K LLQR + N E+ L +A ++ + Q L+ D ++D+ Sbjct: 274 LHCAARNGHVRIIEILLEHGAPIQAKTKNGLSPIHMAAQGDHMDCVRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRMAKVLLDKGAKANARALNGFTPLHIACKKNHMRSMDLLLKHSASLEAVTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVETPLHMASRAGHCEVAQFLLQNAAQVDARAKDDQ 469 HSP 10 Score: 74.3294 bits (181), Expect = 8.218e-14 Identity = 35/156 (22.44%), Postives = 77/156 (49.36%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ 657 G +H+A ++V ++++ G + G P+H A+ + +E+ ++LL+ CE+ G+ P+H AS G +++ +L+ + N+ + G+ P+H + GH+ I L+++ + + M + L +A + V+ L+ Q Sbjct: 567 GLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQ 722 HSP 11 Score: 73.1738 bits (178), Expect = 1.534e-13 Identity = 43/156 (27.56%), Postives = 75/156 (48.08%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 DDQ +H A G E+ ++++D +N + TT G P+H A+ G + + +LL+ T G P+H AS G ++ +LLLER N G+ P+H A +L ++KLL+ + + L +A + Q++ +L+ Sbjct: 467 DDQT--PLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLL 620 HSP 12 Score: 72.0182 bits (175), Expect = 4.389e-13 Identity = 40/177 (22.60%), Postives = 82/177 (46.33%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +EG+ + L + + +G +H+A G V+VA ++++ G+N G+ P+H A L+++ LL+ + +G P+H A+ E+ +LL+ + CE +G+ P+H AS G ++ +L+ ++ ++ N + L L Q + L+ Sbjct: 510 REGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLLERGANPNAAGKNGLTPLHVAVHHNNLDVVKLLVSKGGSAHSTARNGYTPLHIAAKQNQIEVASVLLQNGASPNCESLQGITPLHLASQEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLV 686 HSP 13 Score: 70.8626 bits (172), Expect = 9.683e-13 Identity = 42/162 (25.93%), Postives = 77/162 (47.53%), Query Frame = 1 Query: 178 VDDQGHYSIHIACMGGFVEVARIIIDCG-----SNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 V + G +H+A G + + + ++ G SN++ ET P+H AS G E+ LL+ ++ P+H A+ GH E++KLLL+ N + G P+H A+ GH+ +++LL + + L +A + ++D +LL+ Sbjct: 398 VTESGLTPLHVAAFMGHLNIVKNLLQRGASPNASNVKVET-----PLHMASRAGHCEVAQFLLQNAAQVDARAKDDQTPLHCAARMGHKELVKLLLDHKANPDSATTAGHTPLHIAAREGHIHTIRILLDAGAQQVKMTKKGFTPLHVASKYGKVDVAELLL 554 HSP 14 Score: 66.2402 bits (160), Expect = 2.615e-11 Identity = 37/149 (24.83%), Postives = 70/149 (46.98%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLL 549 FL+ + GN D ++K+ I + G +H+A G V++ ++ G +E T G +H A+ G +++ L+ ++ +++ G P++ A+ H E++K LLE N G P+ A GH ++ LL+ Sbjct: 14 FLRAARSGNLDKALDHIKNGININIANQNGLNGLHLASKEGHVKMVLELLHSGIELEATTKKGNTALHIAALAGQEKVVAELVNYGANVNAQSHKGFSPLYMAAQENHLEVVKFLLENGANQSLPTEDGFTPLAVALQQGHENVVALLI 162 HSP 15 Score: 63.929 bits (154), Expect = 1.595e-10 Identity = 46/195 (23.59%), Postives = 91/195 (46.67%), Query Frame = 1 Query: 118 KEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQ---ILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQ---ELFDFINRN 684 +EG D V + + + G +H+ G V +A ++ G+++ + G P+H A G ++++ LL++ + +T G P+H A+ GH++I+ LLL+ + G P+ A G++ +LKL+ + + + E++ E+ S+ + LL +Q EL DF+ +N Sbjct: 642 QEGRPDMVAMLISKQANVNLGNKNGLTPLHLVAQEGHVGIADTLVKQGASVYAASRMGYTPLHVACHYGNIKMVKFLLQQQAHVNAKTRMGYTPLHQAAQQGHTDIVTLLLKHGALPNEITSNGTSPLGIAKRLGYISVIDVLKLVTEESVSTH-RMSFPETVDEILDVSEDEVILSLLFFLQEEAELLDFVLQN 835
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Match: ENSORLT00000039458.1 (ankyrin 2 [Source:NCBI gene;Acc:101161458]) HSP 1 Score: 92.0485 bits (227), Expect = 1.475e-19 Identity = 54/206 (26.21%), Postives = 102/206 (49.51%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDK 816 ++HIA + A +++ N + ++ G P+H A+ G + + TLLL R ++ +G+ P+H AS G++ +++LLL+R I+ + G+ P+HCA+ GH +++LL++ + + S L +A Q ++CV+ L+ + D++ D + + LHV A C R+ K +LDK Sbjct: 196 ALHIAARKDDTKSAALLLQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPV------DDVTLDYL------------TALHV---AAHCGHYRVTKLLLDK 380 HSP 2 Score: 91.2781 bits (225), Expect = 2.028e-19 Identity = 45/140 (32.14%), Postives = 78/140 (55.71%), Query Frame = 1 Query: 151 LKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRID 570 L++D + G +HIA G V VA ++++ G+ ++ G+ P+H AS RG ++ LLL+R I+ +T G+ P+H A+ GH +++LLE+ + G+ P+H A+ G H++ +K LLQ K +D Sbjct: 213 LQNDHNADVQSKSGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVD 352 HSP 3 Score: 81.2629 bits (199), Expect = 4.889e-16 Identity = 47/182 (25.82%), Postives = 89/182 (48.90%), Query Frame = 1 Query: 103 FLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 FL+ + GN D V +YLK + + G ++H+A G +++ + ++D G+ ++ T G +H +S G E++ L++R DI ++ +G P++ A+ H ++++ LLE N G P+ A GH Q++ +LL+ D K + L +A + LL+ Sbjct: 36 FLRAARAGNIDKVLEYLKGGVDISTCNQNGLNALHLAAKEGHMDLVQELLDRGAPVDSSTKKGNTALHISSLAGQAEVVKTLVKRGADINAQSQNGFTPLYMAAQENHLDVVRYLLENGGNQSTATEDGFTPLAIALQQGHNQVVSILLEN----DTKGKVRLPALHIAARKDDTKSAALLL 213 HSP 4 Score: 79.337 bits (194), Expect = 2.060e-15 Identity = 40/150 (26.67%), Postives = 80/150 (53.33%), Query Frame = 1 Query: 199 SIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 ++H+A G V ++++D +N G P+H A + ++++ LL++ I+ T SG+ PIH A+ GH I+ LLL+ + + + RG +H A+ G ++++ LL+ +D + ++ L +A + + + VQLL+ Sbjct: 361 ALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLL 510 HSP 5 Score: 78.1814 bits (191), Expect = 4.579e-15 Identity = 42/153 (27.45%), Postives = 75/153 (49.02%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +HIAC V+V +++ G++I+ T G+ PIH A+ G L I+ LLL+ + G +H A+ G +E+++ LL ++ P+H AS G +I++LLLQ D + L ++ + Q++ +L+ Sbjct: 391 GFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLL 543 HSP 6 Score: 77.411 bits (189), Expect = 7.771e-15 Identity = 58/229 (25.33%), Postives = 93/229 (40.61%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSG---------------------------------GHSEILKLLLERNCN-------------IECEDNR--------------------GMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDE 690 +H+A G + R+++D GS I+ +T G+ P+H A+ G + +LLE+ + T +G+ P+H A+ G GH + KLLL++ N I C+ NR G+ PIH A+ GHL I+ LLLQ D N E+ L +A ++ Q + V+ L+ + D R D+ Sbjct: 263 LHVASKRGNTNMVRLLLDRGSQIDAKTRDGLTPLHCAARSGHEASVEVLLEKGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRVKVMELLVKYGASIQAVTESGLTPIHVAAFMGHLNIVLLLLQNGASPDVSNIRGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQ 491 HSP 7 Score: 75.485 bits (184), Expect = 2.845e-14 Identity = 45/154 (29.22%), Postives = 70/154 (45.45%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +G S+H+A G EV R ++ G+ ++ P+H AS G EI+ LLL+ + T +G P+H ++ G E +LLE + +G P+H S G L + KLLLQR+ D + L +A LL+ Sbjct: 456 RGETSLHMAARAGQAEVVRCLLRNGAMVDARAREDQTPLHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLL 609 HSP 8 Score: 74.7146 bits (182), Expect = 6.079e-14 Identity = 41/154 (26.62%), Postives = 73/154 (47.40%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLIS 651 G+ +HI+ G +E A ++++ G++ T G P+H S G L++ LLL+R + +G+ P+H A+ + ++ LLL++ + G P+H A+ + I +LLQ + + L LA Q D LL+S Sbjct: 523 GYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMS 676 HSP 9 Score: 68.9366 bits (167), Expect = 4.157e-12 Identity = 40/149 (26.85%), Postives = 71/149 (47.65%), Query Frame = 1 Query: 202 IHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 +HIA G E+ ++++ ++ + TT G P+H ++ G LE ++LLE T G P+H S G ++ KLLL+R + G+ P+H A+ + ++ LLL + + L +A + QMD +L+ Sbjct: 494 LHIASRLGKTEIVQLLLQHMAHPDAATTNGYTPLHISAREGQLETASVLLEAGASHSLATKKGFTPLHVVSKYGSLDVAKLLLQRQAPPDSAGKNGLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLL 642 HSP 10 Score: 65.0846 bits (157), Expect = 6.056e-11 Identity = 35/153 (22.88%), Postives = 71/153 (46.41%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G+ +HIA +++A +++ G+ T G+ P+H AS G ++ LL+ + I T SG+ +H A+ + ++L+ N++ + G P+ A G+ +++ LLQ ++ K + L A Q + +L+ Sbjct: 622 GYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLL 774 HSP 11 Score: 64.3142 bits (155), Expect = 1.248e-10 Identity = 35/153 (22.88%), Postives = 73/153 (47.71%), Query Frame = 1 Query: 190 GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLI 648 G +H+A +VA +++D G++ G P+H A+ + ++I T+LL+ + T G+ P+H AS GH+++ LL+ + I G+ +H A+ + + ++L++ +D + + + L +A V L+ Sbjct: 589 GLTPLHVAAHYDNQKVALLLLDKGASPHTTAKNGYTPLHIAAKKNQMDIATVLLQYGAETNALTKQGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLL 741 HSP 12 Score: 59.6918 bits (143), Expect = 3.425e-9 Identity = 29/122 (23.77%), Postives = 63/122 (51.64%), Query Frame = 1 Query: 187 QGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ 552 QG +H+A G ++A +++ G+ I T G+ +H A+ + + +L+ +++ +T G P+ A G+++++ LL+ ++ + G P+H A+ G+ I+ +LLQ Sbjct: 654 QGVTPLHLASQEGHADMAALLMSKGAQINVPTKSGLTALHLAAQEDRVAVAEILVRNGANLDHQTKLGYTPLIVACHYGNAKMVSFLLQNGASVNAKTKNGYTPLHQAAQQGNTHIINVLLQ 775
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Match: SMESG000019753.1 (SMESG000019753.1) HSP 1 Score: 630.172 bits (1624), Expect = 0.000e+0 Identity = 315/350 (90.00%), Postives = 316/350 (90.29%), Query Frame = 1 Query: 85 MGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNS---------------------------------GMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYFN 1035 MGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSR CLEILTLLLERNCDIECETNS GMRPIHYASSGGHSEILKLL+ERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYFN Sbjct: 1 MGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRSCLEILTLLLERNCDIECETNSRMRPIHYASSGGHSEILKLLVERNCNIECEDNRGMRPIHYASSGGHSEILKLLVERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYFN 350
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Match: SMESG000043462.1 (SMESG000043462.1) HSP 1 Score: 322.398 bits (825), Expect = 3.017e-108 Identity = 173/353 (49.01%), Postives = 220/353 (62.32%), Query Frame = 1 Query: 85 MGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNC---------------------------------NIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDD----EEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYF 1032 MGDFQCF +HL GN +GV LK +F++ IVD+ G++ IHI C GF+ V R+++DC NIECE GMRPIH+AS G LEIL LLL+RNC+IECE G PIHYAS GHSEILKLLL+RNC NIECE+ G R +H ASS GH +ILKLLLQRKCR DCK +S L+LA Q +DCV+LL V++ F I R+D I + ++ S +FI+ NIY+GL+VL+MAI NA++IVKCILDK+VD+ E N E+N+ D +C+IC + PV I FQCGHL C CS + C C+A I R K++F Sbjct: 1 MGDFQCFKEHLMNGNVEGVRNNLKWEFVDKIVDNDGNFPIHIVCRNGFLSVVRLLLDCNCNIECEDKCGMRPIHWASENGHLEILKLLLDRNCNIECENRWGRIPIHYASGNGHSEILKLLLDRNCNIECQDKWGNRPIHWACRNGHSEILKLLLDRNCNIECENKSGRRSVHFASSNGHSEILKLLLQRKCRFDCKTKDGKSPLDLAKQFNNVDCVELLTHVEKFFAIIARDDPIEIENLFISFNFIQPNIYTGLNVLEMAINNNAEKIVKCILDKSVDNKAEIERSHRNNAKLSKEENQPDNEIPMCHICQDNPVSIGFQCGHLTCNVCSDQLFECPYCKADIVSRIKMFF 353
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Match: SMESG000010464.1 (SMESG000010464.1) HSP 1 Score: 296.204 bits (757), Expect = 9.100e-92 Identity = 152/257 (59.14%), Postives = 184/257 (71.60%), Query Frame = 1 Query: 64 NSKKYFMMGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEE 834 N K MGDF F QHL GNA+GV + L+ +F IVD G + IHIAC GFV+ ARI++D NIECET GMRPIH+AS +G EI+ LL +R C IECE SG RPIH A++ GHSEILKLLLERNCNIECED G RPIH ASSGGH ++LKLLLQRKC I+CK S L+LA + + CVQLL V+ LF I +D IAF + KSSDFIR+N+Y+GL VL+ AI+ NA+ I+KCILDK +DD+E Sbjct: 27 NQKGNIKMGDFIYFKQHLMNGNAEGVRENLRREFAYKIVDKHGKFPIHIACRDGFVDTARILLDINCNIECETKSGMRPIHWASMKGHSEIVKLLNDRYCSIECEDKSGSRPIHCATTEGHSEILKLLLERNCNIECEDKWGRRPIHLASSGGHSELLKLLLQRKCLINCKTKSGMSPLDLAKKFNRAKCVQLLTHVENLFAIIEWDDPIAFYDLCKSSDFIRQNLYTGLAVLEKAIDSNANNIIKCILDKLIDDKE 283
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Match: SMESG000010464.1 (SMESG000010464.1) HSP 1 Score: 295.434 bits (755), Expect = 2.427e-91 Identity = 152/257 (59.14%), Postives = 184/257 (71.60%), Query Frame = 1 Query: 64 NSKKYFMMGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEE 834 N K MGDF F QHL GNA+GV + L+ +F IVD G + IHIAC GFV+ ARI++D NIECET GMRPIH+AS +G EI+ LL +R C IECE SG RPIH A++ GHSEILKLLLERNCNIECED G RPIH ASSGGH ++LKLLLQRKC I+CK S L+LA + + CVQLL V+ LF I +D IAF + KSSDFIR+N+Y+GL VL+ AI+ NA+ I+KCILDK +DD+E Sbjct: 27 NQKGNIKMGDFIYFKQHLMNGNAEGVRENLRREFAYKIVDKHGKFPIHIACRDGFVDTARILLDINCNIECETKSGMRPIHWASMKGHSEIVKLLNDRYCSIECEDKSGSRPIHCATTEGHSEILKLLLERNCNIECEDKWGRRPIHLASSGGHSELLKLLLQRKCLINCKTKSGMSPLDLAKKFNRAKCVQLLTHVENLFAIIEWDDPIAFYDLCKSSDFIRQNLYTGLAVLEKAIDSNANNIIKCILDKLIDDKE 283 HSP 2 Score: 65.4698 bits (158), Expect = 3.344e-11 Identity = 46/173 (26.59%), Postives = 87/173 (50.29%), Query Frame = 1 Query: 547 LQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWK--SSDFIRENIYSGLHVLDMAIECNAD--RIV---KCILDKTVD---DEEPKDTNDHKDSEKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYFN 1035 + +K ++ KN ++S ++LAI++ Q+D + LLI E D + N + A ++ + S N + + A+ D +I + ILD + E ++ + + + N C IC++ P+++ FQCGHL C+ C+ + CH+CR +I + K++FN Sbjct: 817 INKKKFVNLKNRRNQSAVQLAIKADQLDLIDLLIHESEGLDCEDTNGKSALGQLMEKLSISNNEHNHERLISAVKSAMRSGGDIYKITTKGRRILDSCTNMDAKAEIEEFHRRQPTNINPTGNEANTCTICMDKPLNMVFQCGHLCCSDCADQIFECHMCRIEILTKTKLFFN 989
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Match: SMESG000019959.1 (SMESG000019959.1) HSP 1 Score: 273.478 bits (698), Expect = 4.231e-83 Identity = 144/284 (50.70%), Postives = 190/284 (66.90%), Query Frame = 1 Query: 85 MGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQ-GHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLL---------------------------------LQRKCRIDCKNNMSESILELAIQSKQMDCVQLLISVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDEE 834 MGDFQ F +HL +GNA+ V L F++ IVDD+ G++ IHIAC GFVEVA++++D N CET G RPIHYAS +G EI+ LLL+RNC+ ECE SG RPIHYAS GHSEI+KLLL+RNCN ECE GMRPIH AS GH +I+KLL LQRKCRIDCKN +S L+ A + ++DC++LL ++++LF I R+D I +K++ S +FIRENIY+GL+VL+MA+ NAD+I+K ILDK++D++E Sbjct: 1 MGDFQSFKKHLMKGNAERVRNNLSGGFVDKIVDDKYGYFPIHIACRDGFVEVAKLLLDRNCNTICETKSGDRPIHYASWQGHSEIVKLLLDRNCNTECENESGNRPIHYASWQGHSEIVKLLLDRNCNTECETKSGMRPIHYASWNGHSEIVKLLLDRNCNTECENESGNRPIHYASREGHSEIVKLFLQRKCRIDCKNKDGKSPLDFAKEYNRVDCMKLLTNMEKLFAIIERDDPIEIEKLFISCEFIRENIYTGLNVLEMAMTSNADKIIKWILDKSIDNKE 284 HSP 2 Score: 120.939 bits (302), Expect = 1.953e-29 Identity = 83/265 (31.32%), Postives = 127/265 (47.92%), Query Frame = 1 Query: 181 DDQGHYSIHIACMGGFVEVARIIIDCGSNIECETTWGMRPIHYASSRGCLEILTLLLERNCDIECETNSGMRPIHYASSGGHSEILKLLLERNCNIECEDNRGMRPIHCASSGGHLQILKLLLQ----------------RKCRIDCK---------------------NNMSESILELAIQSKQMDCVQLLISVQE-----------LFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDE 831 ++ G+ IH A G E+ ++++D N ECET GMRPIHYAS G EI+ LLL+RNC+ ECE SG RPIHYAS GHSEI+KL L+R C I+C++ G P+ A + +KLL K I C+ + + + IL+ +I +K++D V ++ ++ L+ I R+ F + + + G L++A+E D ++ IL KT+ D+ Sbjct: 100 NESGNRPIHYASWQGHSEIVKLLLDRNCNTECETKSGMRPIHYASWNGHSEIVKLLLDRNCNTECENESGNRPIHYASREGHSEIVKLFLQRKCRIDCKNKDGKSPLDFAKEYNRVDCMKLLTNMEKLFAIIERDDPIEIEKLFISCEFIRENIYTGLNVLEMAMTSNADKIIKWILDKSIDNKEIDEVIKELTAKDKENANLLKERLLYLCITRHHMRGFKRFHSAEVINGQRRTVGPTFLELAVENGCDLLIDLILRKTIYDK 364 HSP 3 Score: 56.9954 bits (136), Expect = 1.720e-8 Identity = 48/163 (29.45%), Postives = 76/163 (46.63%), Query Frame = 1 Query: 598 LELAIQSKQMDCVQLLI---------------SVQELFDFINRNDEIAFDKIWKSSDFIRENIYSGLHVLDMAIECNADRIVKCILDKTVDDE-EPKDTNDHKDS-EKNEDDKLRGICNICLEYPVDIAFQCGHLICTPCSLRASSCHVCRAQITDRRKIYFN 1035 ++AI+ ++MD V LI ++ +L + I+ ++ + F + S I+ I G + M RI+ D +E E N+ K S E+N+ D +C IC V+I FQCGHLIC CS + C C+A I R K++F+ Sbjct: 834 FQIAIEIEEMDLVNALIIETKDCHSEDEGGTCALTQLLEKISISNNVNFKRKLILS--IKSIISLGGDIYKMTSR-GQHRIIDACTDSVAKEEIERYHRNNAKPSKEENKPDNEIPMCKICQNKQVNIGFQCGHLICDLCSDQLFECPFCKADIVTRTKMFFS 993 The following BLAST results are available for this feature:
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 4
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of E3 ubiquitin-protein ligase RGLG4 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010632 ID=SMED30010632|Name=E3 ubiquitin-protein ligase RGLG4|organism=Schmidtea mediterranea sexual|type=transcript|length=1172bpback to top protein sequence of SMED30010632-orf-1 >SMED30010632-orf-1 ID=SMED30010632-orf-1|Name=SMED30010632-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=319bp MMGDFQCFLQHLKEGNADGVWKYLKSDFLNTIVDDQGHYSIHIACMGGFVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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