SMED30010591
Overview
Neoblast Clusters
Zeng et. al., 2018
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny AppEmbryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010591 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 2
Alignments
SMED30010591 aligns in the following genomic locations:
Homology
BLAST of SMED30010591 vs. UniProt/SwissProt
Match: sp|Q9M022|AIRP2_ARATH (E3 ubiquitin-protein ligase AIRP2 OS=Arabidopsis thaliana OX=3702 GN=AIRP2 PE=1 SV=1) HSP 1 Score: 51.9878 bits (123), Expect = 2.367e-6 Identity = 48/185 (25.95%), Postives = 74/185 (40.00%), Query Frame = 1 Query: 448 IRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRP------------DCPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCI-ENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEGNKRID-----------ENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPF 930 +R S ++ L + A I+F + S +P + + ++ + Y P DC ++ + L RI I L E KT I E Y + + L GIT R K+I + + R D E +C ICL++ NK L C H +C C ++ SCPF Sbjct: 1 MRKSFKDSLKALEADIQFANTLASEYPEEYDGGYVQMRLSYSPAAHLFLFLLQWTDCHFAGALGLLRILIYKAYVDGKTTMSLHERKTSIREFYDVLFPSLLQLHGGITDVEERKQKEICDKRYRKKDRTDKGKMSEIDLEREEECGICLEIRNK---VVLPTCNHSMCINCYRNWRARSQSCPF 182
BLAST of SMED30010591 vs. Planmine SMEST
Match: SMESG000075750.1 (SMESG000075750.1) HSP 1 Score: 714.531 bits (1843), Expect = 0.000e+0 Identity = 353/355 (99.44%), Postives = 353/355 (99.44%), Query Frame = 1 Query: 22 MDFDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFTLNESRTLLWCQNQAFSNEELKIQDELECYWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPDCPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEGNKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAFFNGNNRGMSKLIQDFLEIDHFLD 1086 MDFDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFTLNESRTLLWCQNQAFSNEELKIQDELE YWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPDCPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHE NKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAFFNGNNRGMSKLIQDFLEIDHFLD Sbjct: 1 MDFDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFTLNESRTLLWCQNQAFSNEELKIQDELESYWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPDCPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEVNKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAFFNGNNRGMSKLIQDFLEIDHFLD 355
BLAST of SMED30010591 vs. Planmine SMEST
Match: SMESG000030485.1 (SMESG000030485.1) HSP 1 Score: 99.3673 bits (246), Expect = 4.633e-23 Identity = 81/329 (24.62%), Postives = 154/329 (46.81%), Query Frame = 1 Query: 46 CSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFT-----------LNESRTLLWCQNQAFSNEELKIQDELECYWKSYVSVIIQSLHFYIDTLEKVDLMLDT-------WISHAIPFDFYQINEFYNFINENLALIRNSL-EEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNII--FYRPDCPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEGNKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFEN 969 CS+CYDS+ + + + C H+ C C + +VF++ C D + I +V + + ++L +N+ S E I +++ W +++HF L+K ++L+ W++ D +++F I + +SL + D+ K ++ ++ + P + +PF+ NI+ Y + LI+ +F ID +QN L + I + N +L T+S+ +N+++YE + + +++ID + CLICL I AKLS CQHKIC C++ F + + SCPF ++ + + Sbjct: 11 CSICYDSLKNCDSVVLPDCLHRFCRNCANEIVFKRVAKCQLDNRRINAVINCIIDTNGKNQLIIKVKSLKQYRNKILSMEPQSITEDIMNTWAE------RTMHFSC-VLDKTSVILNLLKNKFTLWMAEKTAIDSETVSQFNYAITHKMVNELDSLYKADIFKLTDKERYYRKYANRHPTKCISPFLINILNSVYIYE-TIDLIMPVFCCLID---SGIVKQNYLLKCVKAIGKHFEHLRNISNYLISFTLSFLNNNREVYEKLQKCHRQIDIDNCLICLCQIYFYPHAKLSNCQHKICHSCIDQFLITYASCPFHDATITQLTLVTD 328
BLAST of SMED30010591 vs. Planmine SMEST
Match: SMESG000067204.1 (SMESG000067204.1) HSP 1 Score: 100.523 bits (249), Expect = 6.556e-23 Identity = 103/385 (26.75%), Postives = 173/385 (44.94%), Query Frame = 1 Query: 28 FDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFTLNESR---------TLLWCQNQAFSNEELKIQDELECYWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPD---CPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEGNKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAFFNG----------------NNRGMSKLIQ--DF--LEIDHFLD 1086 FD E C +CYD NN F+ C H+IC C +VF+ + ICPFDRQ I + ++E+ ++ + Q++A ++ I + +E W S++S + SL I+ +E + L L I A D F + EN + + + L A + K + S + + IN + ++ P L +L + ++ N ++N+ + IE +I S L I + + N I++ I G+ + + CLIC+ I K K++K + C HKIC C F + HG CPFD +LS +F ++ + + +S + F NNR + LI+ DF L I++F+D Sbjct: 4 FDNEKICLICYDE-GKNNFVSFKSCNHRICKNCSFEVVFQNQPICPFDRQEI--SYVIDETNLDIKNSQPLSIEYYQSRAVKSDPEIIINIIEATWSSFLSQLNDSLKTLIEIIESM-LKLHKEILKAKLIDLNNKLTF-ELLMENFNDVLDIFNQTLNCTKATEIYCKSLESDSYSELSLTQINIVNAHKVLINLSPLELNYHL--VLANMTNLGSIEKNIETMETDLIELKEISLSEKCLL-----IMFVKSNGQIFKSIFPGSDQHPIDSCLICVQPIKKLKYSKFNNCAHKICLACCYKFMISHGRCPFDGTELSELNVFVRMKNSDFDLKSKKTSVFILNRIEDLCFQLFKNIIEFNNRKIKTLIEKNDFFVLLINYFID 376
BLAST of SMED30010591 vs. Planmine SMEST
Match: SMESG000067204.1 (SMESG000067204.1) HSP 1 Score: 100.523 bits (249), Expect = 6.896e-23 Identity = 103/385 (26.75%), Postives = 173/385 (44.94%), Query Frame = 1 Query: 28 FDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDRQPIFSVFTLNESR---------TLLWCQNQAFSNEELKIQDELECYWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPD---CPWSLIVYLFRISIDLKNKKETQQNLLSEAKTCIENYKIKSSNFLLFLTGITISYFRDNKDIYEGIHEGNKRIDENQCLICLDLINKNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAF----------------FNGNNRGMSKLIQ--DF--LEIDHFLD 1086 FD E C +CYD NN F+ C H+IC C +VF+ + ICPFDRQ I + ++E+ ++ + Q++A ++ I + +E W S++S + SL I+ +E + L L I A D F + EN + + + L A + K + S + + IN + ++ P L +L + ++ N ++N+ + IE +I S L I + + N I++ I G+ + + CLIC+ I K K++K + C HKIC C F + HG CPFD +LS +F ++ + + +S + F NNR + LI+ DF L I++F+D Sbjct: 2 FDNEKICLICYDE-GKNNFVSFKSCNHRICKNCSFEVVFQNQPICPFDRQEI--SYVIDETNLDIKNSQPLSIEYYQSRAVKSDPEIIINIIEATWSSFLSQLNDSLKTLIEIIESM-LKLHKEILKAKLIDLNNKLTF-ELLMENFNDVLDIFNQTLNCTKATEIYCKSLESDSYSELSLTQINIVNAHKVLINLSPLELNYHL--VLANMTNLGSIEKNIETMETDLIELKEISLSEKCLL-----IMFVKSNGQIFKSIFPGSDQHPIDSCLICVQPIKKLKYSKFNNCAHKICLACCYKFMISHGRCPFDGTELSELNVFVRMKNSDFDLKSKKTSVFILNRIEDLCFQLFKNIIEFNNRKIKTLIEKNDFFVLLINYFID 374
BLAST of SMED30010591 vs. Planmine SMEST
Match: SMESG000067182.1 (SMESG000067182.1) HSP 1 Score: 97.0561 bits (240), Expect = 7.781e-22 Identity = 98/376 (26.06%), Postives = 159/376 (42.29%), Query Frame = 1 Query: 22 MDFDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDR---QPIFSVFTLNES-------------RTLLWCQNQAFSNEELKIQDELECYWKSYVSVIIQSLHFYIDTLEKVDLMLDTWISHAIPFDFYQINEFYNFINENLALIRNSLEEDLPSKPAKIKFIKEVLSHFPRQNNTPFINNIIFYRPDCPWSLIVYLFRISIDLKNKKE----TQQNLLSEAKTCIENYKIKSSNFLLF------LTGITISYFRDNKDIYEGIHEGNKRIDENQCLICLDLIN-KNKFAKLSECQHKICEKCVNDFGVLHGSCPFDSDQLSNFIIFENDRSIESEFQSTFNTAFFNGNNRGMSKLIQDFLE 1068 MD++F+T C +CYD+++ +N+ +C H C C+ L+F + CP DR Q + F L ++ TL + F + +I E Y+ Q+L+ I+ ++ V W +I ++NE L LI E S K+ F K+ S F R ++ + + + ++ L ++ I L+N N + +++ I+ ++ F + ISY N+D+Y I K D N+CLIC I K K++K ++C HKIC C +F + H SCPFDSD L I + + Q + N N+ SK IQD E Sbjct: 1 MDYEFDTTCVICYDNLETSNVVTLNKCPHVFCRSCVKELIFSEDPRCPLDRIEIQVVHDPFLLKQNLDFTEFKPISKYIETLFIFEADYFLTDIREIISE-------YLLKFNQNLNQIIEAMKDVQTFYRKW---SINRQTLKLNEIRTL----LTLIEIIRSEITKSDNIKLFFCKKS-SFFLR-----YLKGLTEEKIRLEYINLLNLDKLKIKLRNSTSHCICLTNNTIQQSEVLIKYMEVIDEELTEFQQISNYAKCLIISYVPKNRDLYSKIKPNIKENDTNKCLICETEIKLKGKYSKFNQCDHKICILCTENFMITHASCPFDSDDLIGLEICDQSENTLINSQEYLLRNYLNIGNKLFSK-IQDLNE 355 The following BLAST results are available for this feature:
BLAST of SMED30010591 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30010591 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 1
BLAST of SMED30010591 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30010591 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30010591 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010591 ID=SMED30010591|Name=SMED30010591|organism=Schmidtea mediterranea sexual|type=transcript|length=1107bpback to top protein sequence of SMED30010591-orf-1 >SMED30010591-orf-1 ID=SMED30010591-orf-1|Name=SMED30010591-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=356bp MDFDFETCCSVCYDSMDDNNIAIFQQCRHKICIICLHHLVFEKKFICPFDback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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