Short coiled-coil protein homolog

Overview
NameShort coiled-coil protein homolog
Smed IDSMED30010576
Length (bp)672
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of Short coiled-coil protein homolog (SMED30010576) t-SNE clustered cells

Violin plots show distribution of expression levels for Short coiled-coil protein homolog (SMED30010576) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of Short coiled-coil protein homolog (SMED30010576) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for Short coiled-coil protein homolog (SMED30010576) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010576

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 3

  
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Match: SCOC (short coiled-coil protein [Source:HGNC Symbol;Acc:HGNC:20335])

HSP 1 Score: 98.5969 bits (244), Expect = 2.468e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Match: SCOC (short coiled-coil protein [Source:HGNC Symbol;Acc:HGNC:20335])

HSP 1 Score: 98.5969 bits (244), Expect = 2.468e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Match: SCOC (short coiled-coil protein [Source:HGNC Symbol;Acc:HGNC:20335])

HSP 1 Score: 98.5969 bits (244), Expect = 2.468e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Match: SCOC (short coiled-coil protein [Source:HGNC Symbol;Acc:HGNC:20335])

HSP 1 Score: 98.5969 bits (244), Expect = 2.468e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Match: SCOC (short coiled-coil protein [Source:HGNC Symbol;Acc:HGNC:20335])

HSP 1 Score: 98.5969 bits (244), Expect = 2.468e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Celegans
Match: unc-69 (Short coiled-coil domain-containing protein UNC-69 splice variant T07A5.6b [Source:UniProtKB/TrEMBL;Acc:G5EDQ5])

HSP 1 Score: 67.781 bits (164), Expect = 1.265e-14
Identity = 31/47 (65.96%), Postives = 42/47 (89.36%), Query Frame = 3 
Query:  243 KQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLM 383
            K ++I+Q++ELQ+TL+DLS RVE VKEE++KLRSENQ+L QY +NLM
Sbjct:   46 KARMITQVLELQNTLDDLSQRVESVKEESLKLRSENQVLGQYIQNLM 92
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Celegans
Match: unc-69 (Short coiled-coil domain-containing protein UNC-69 splice variant T07A5.6b [Source:UniProtKB/TrEMBL;Acc:G5EDQ5])

HSP 1 Score: 67.3958 bits (163), Expect = 2.016e-14
Identity = 31/47 (65.96%), Postives = 42/47 (89.36%), Query Frame = 3 
Query:  243 KQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLM 383
            K ++I+Q++ELQ+TL+DLS RVE VKEE++KLRSENQ+L QY +NLM
Sbjct:   46 KARMITQVLELQNTLDDLSQRVESVKEESLKLRSENQVLGQYIQNLM 92
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Celegans
Match: unc-69 (Short coiled-coil domain-containing protein UNC-69 splice variant T07A5.6b [Source:UniProtKB/TrEMBL;Acc:G5EDQ5])

HSP 1 Score: 67.781 bits (164), Expect = 4.825e-14
Identity = 31/47 (65.96%), Postives = 42/47 (89.36%), Query Frame = 3 
Query:  243 KQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLM 383
            K ++I+Q++ELQ+TL+DLS RVE VKEE++KLRSENQ+L QY +NLM
Sbjct:   46 KARMITQVLELQNTLDDLSQRVESVKEESLKLRSENQVLGQYIQNLM 92
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Fly
Match: CG5934 (gene:FBgn0039505 transcript:FBtr0085158)

HSP 1 Score: 93.9745 bits (232), Expect = 3.107e-24
Identity = 49/86 (56.98%), Postives = 67/86 (77.91%), Query Frame = 3 
Query:  192 ASTDNKNH-----MEIS-DSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +ST + +H     ME++ D  +EK +LI+Q++ELQ+TL+DLS RV+ VKEEN+KLRSENQ+L QY ENLMSASSVFQ+ SP   ++
Sbjct:   49 SSTPDSSHNSLEAMEMAQDDREEKARLITQVLELQNTLDDLSQRVDSVKEENLKLRSENQVLGQYIENLMSASSVFQSTSPSAAKK 134
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Zebrafish
Match: scoca (short coiled-coil protein a [Source:NCBI gene;Acc:562638])

HSP 1 Score: 96.2857 bits (238), Expect = 1.017e-25
Identity = 45/65 (69.23%), Postives = 57/65 (87.69%), Query Frame = 3 
Query:  237 QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   16 EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 80
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Zebrafish
Match: scoca (short coiled-coil protein a [Source:NCBI gene;Acc:562638])

HSP 1 Score: 96.2857 bits (238), Expect = 1.175e-25
Identity = 45/65 (69.23%), Postives = 57/65 (87.69%), Query Frame = 3 
Query:  237 QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   15 EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 79
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Zebrafish
Match: scoca (short coiled-coil protein a [Source:NCBI gene;Acc:562638])

HSP 1 Score: 97.0561 bits (240), Expect = 1.448e-25
Identity = 45/65 (69.23%), Postives = 57/65 (87.69%), Query Frame = 3 
Query:  237 QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   55 EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 119
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Zebrafish
Match: scocb (short coiled-coil protein b [Source:NCBI gene;Acc:791709])

HSP 1 Score: 93.9745 bits (232), Expect = 8.531e-25
Identity = 44/66 (66.67%), Postives = 58/66 (87.88%), Query Frame = 3 
Query:  234 IQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++EK +LI+Q++ELQ+TLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   15 LEEKTRLINQVLELQNTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDSKSKRK 80
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Xenopus
Match: SCOC (short coiled-coil protein [Source:NCBI gene;Acc:100494794])

HSP 1 Score: 96.6709 bits (239), Expect = 3.586e-25
Identity = 45/66 (68.18%), Postives = 58/66 (87.88%), Query Frame = 3 
Query:  234 IQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   60 LEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 125
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Xenopus
Match: SCOC (short coiled-coil protein [Source:NCBI gene;Acc:100494794])

HSP 1 Score: 96.6709 bits (239), Expect = 5.890e-25
Identity = 45/66 (68.18%), Postives = 58/66 (87.88%), Query Frame = 3 
Query:  234 IQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   75 LEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 140
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Mouse
Match: Scoc (short coiled-coil protein [Source:MGI Symbol;Acc:MGI:1927654])

HSP 1 Score: 98.5969 bits (244), Expect = 1.694e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Mouse
Match: Scoc (short coiled-coil protein [Source:MGI Symbol;Acc:MGI:1927654])

HSP 1 Score: 98.5969 bits (244), Expect = 1.694e-26
Identity = 48/79 (60.76%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    8 AVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 82
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Mouse
Match: Scoc (short coiled-coil protein [Source:MGI Symbol;Acc:MGI:1927654])

HSP 1 Score: 97.8265 bits (242), Expect = 2.977e-26
Identity = 50/85 (58.82%), Postives = 67/85 (78.82%), Query Frame = 3 
Query:  183 LINAS--TDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++NA    D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    1 MMNADMDVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 81
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Mouse
Match: Scoc (short coiled-coil protein [Source:MGI Symbol;Acc:MGI:1927654])

HSP 1 Score: 98.9821 bits (245), Expect = 3.584e-26
Identity = 50/87 (57.47%), Postives = 69/87 (79.31%), Query Frame = 3 
Query:  180 QLINA---STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +++NA   + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   43 KMMNADMDAVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 125
[Close]
BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Match: sp|Q5RJZ6|SCOC_RAT (Short coiled-coil protein OS=Rattus norvegicus OX=10116 GN=Scoc PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.306e-25
Identity = 54/107 (50.47%), Postives = 75/107 (70.09%), Query Frame = 3 
Query:  120 DYDKPKNEIKMGSLNFSPDNQLINA---STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            D D     +  G+    P  +++NA   + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   22 DTDHSLKSLHSGAERLFP--KMMNADMDAVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 122
[Close]
BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Match: sp|Q78YZ6|SCOC_MOUSE (Short coiled-coil protein OS=Mus musculus OX=10090 GN=Scoc PE=1 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 2.507e-25
Identity = 50/87 (57.47%), Postives = 69/87 (79.31%), Query Frame = 3 
Query:  180 QLINA---STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +++NA   + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   43 KMMNADMDAVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 125
[Close]
BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Match: sp|Q9UIL1|SCOC_HUMAN (Short coiled-coil protein OS=Homo sapiens OX=9606 GN=SCOC PE=1 SV=2)

HSP 1 Score: 98.9821 bits (245), Expect = 7.101e-25
Identity = 53/106 (50.00%), Postives = 75/106 (70.75%), Query Frame = 3 
Query:  123 YDKPKNEIKMGSLNFSPDNQLINA---STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            Y +PK+ +           +++NA   + D +N +E+    +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   68 YPRPKSLLP----------KMMNADMDAVDAENQVEL----EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 159
[Close]
BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Match: sp|Q08BG7|SCOCA_DANRE (Short coiled-coil protein A OS=Danio rerio OX=7955 GN=scoca PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.093e-24
Identity = 45/65 (69.23%), Postives = 57/65 (87.69%), Query Frame = 3 
Query:  237 QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   55 EEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 119
[Close]
BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Match: sp|Q5XJK1|SCOCB_DANRE (Short coiled-coil protein B OS=Danio rerio OX=7955 GN=scocb PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 7.076e-24
Identity = 44/66 (66.67%), Postives = 58/66 (87.88%), Query Frame = 3 
Query:  234 IQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++EK +LI+Q++ELQ+TLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   11 LEEKTRLINQVLELQNTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDSKSKRK 76
[Close]
BLAST of Short coiled-coil protein homolog vs. TrEMBL
Match: A0A2D0QNE2 (short coiled-coil protein B-like isoform X1 OS=Ictalurus punctatus OX=7998 GN=LOC108263532 PE=4 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 2.979e-26
Identity = 57/112 (50.89%), Postives = 75/112 (66.96%), Query Frame = 3 
Query:   96 IPLSSDESDYDKPKNEIKMGSLNFSPDNQLINASTDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            I L+ D +D D         +L   P+ QL   + D    ME    ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   15 ISLADDSADCDS-------AALRPKPEEQLSTMNCDMDGDMENQVEVEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 119
[Close]
BLAST of Short coiled-coil protein homolog vs. TrEMBL
Match: A0A4W5NEB0 (Short coiled-coil protein OS=Hucho hucho OX=62062 GN=SCOC PE=4 SV=1)

HSP 1 Score: 105.916 bits (263), Expect = 5.270e-26
Identity = 51/80 (63.75%), Postives = 64/80 (80.00%), Query Frame = 3 
Query:  195 STDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRRG 434
            S+D    ME    ++EK +LI+Q++ELQHTLEDLSSRV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+G
Sbjct:    2 SSDGDGDMENQAELEEKTRLINQVLELQHTLEDLSSRVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRKG 81
[Close]
BLAST of Short coiled-coil protein homolog vs. TrEMBL
Match: A0A5N5PPB6 (Uncharacterized protein OS=Pangasianodon hypophthalmus OX=310915 GN=PHYPO_G00175620 PE=4 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 7.646e-26
Identity = 53/93 (56.99%), Postives = 68/93 (73.12%), Query Frame = 3 
Query:  153 GSLNFSPDNQLINASTDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
             +L   PD QL   + D    ME    ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   37 AALRPRPDEQLSTMNCDMDGDMENQVEVEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 129
[Close]
BLAST of Short coiled-coil protein homolog vs. TrEMBL
Match: A0A3P8PIB7 (Short coiled-coil protein a OS=Astatotilapia calliptera OX=8154 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.593e-25
Identity = 52/93 (55.91%), Postives = 69/93 (74.19%), Query Frame = 3 
Query:  153 GSLNFSPDNQLINASTDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
             +L    +N+LI  + D    ME    ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   27 AALYTKQENELITMNCDIDGDMENQVEVEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 119
[Close]
BLAST of Short coiled-coil protein homolog vs. TrEMBL
Match: A0A3P9BZJ9 (Short coiled-coil protein a OS=Maylandia zebra OX=106582 PE=4 SV=1)

HSP 1 Score: 105.145 bits (261), Expect = 2.593e-25
Identity = 52/93 (55.91%), Postives = 69/93 (74.19%), Query Frame = 3 
Query:  153 GSLNFSPDNQLINASTDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
             +L    +N+LI  + D    ME    ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   27 AALYTKQENELITMNCDIDGDMENQVEVEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 119
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Cavefish
Match: scoca (short coiled-coil protein B-like [Source:NCBI gene;Acc:103034491])

HSP 1 Score: 98.2117 bits (243), Expect = 5.285e-26
Identity = 45/66 (68.18%), Postives = 58/66 (87.88%), Query Frame = 3 
Query:  234 IQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:   53 LEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 118
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Cavefish
Match: scocb (short coiled-coil protein b [Source:NCBI gene;Acc:791709])

HSP 1 Score: 94.7449 bits (234), Expect = 3.431e-25
Identity = 47/78 (60.26%), Postives = 62/78 (79.49%), Query Frame = 3 
Query:  198 TDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +D    ME    ++EK +LI+Q++ELQ+TLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    4 SDVDGDMENQVELEEKTRLINQVLELQNTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDSKSKRK 81
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Cavefish
Match: scocb (short coiled-coil protein b [Source:NCBI gene;Acc:791709])

HSP 1 Score: 93.5893 bits (231), Expect = 8.293e-25
Identity = 46/72 (63.89%), Postives = 60/72 (83.33%), Query Frame = 3 
Query:  216 MEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            ME    ++EK +LI+Q++ELQ+TLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    5 MENQVELEEKTRLINQVLELQNTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDSKSKRK 76
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Sea Lamprey
Match: scocb (short coiled-coil protein b [Source:NCBI gene;Acc:791709])

HSP 1 Score: 97.8265 bits (242), Expect = 4.014e-27
Identity = 46/68 (67.65%), Postives = 59/68 (86.76%), Query Frame = 3 
Query:  228 DSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            + ++EK +LI+Q++ELQHTLEDLSSRV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    6 EEVEEKTRLINQVLELQHTLEDLSSRVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDSKSKRK 73
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Nematostella
Match: EDO49602 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RFH2])

HSP 1 Score: 84.3445 bits (207), Expect = 1.266e-21
Identity = 39/65 (60.00%), Postives = 55/65 (84.62%), Query Frame = 3 
Query:  237 QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
            +EK ++IS+++ELQ+TL+DLS RV+ VK+EN+KL+SENQ+L QY ENLMSASSVFQ  +  + +R
Sbjct:    8 EEKARIISEVLELQNTLDDLSQRVDAVKDENLKLKSENQVLGQYIENLMSASSVFQPTNTGSKKR 72
[Close]
BLAST of Short coiled-coil protein homolog vs. Ensembl Medaka
Match: scocb (short coiled-coil protein [Source:NCBI gene;Acc:101156868])

HSP 1 Score: 97.4413 bits (241), Expect = 3.364e-26
Identity = 48/78 (61.54%), Postives = 61/78 (78.21%), Query Frame = 3 
Query:  198 TDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRR 431
             D    ME    ++EK +LI+Q++ELQHTLEDLS+RV+ VKEEN+KL+SENQ+L QY ENLMSASSVFQT   K+ R+
Sbjct:    3 CDIDGDMENQVEMEEKTRLINQVLELQHTLEDLSARVDAVKEENLKLKSENQVLGQYIENLMSASSVFQTTDTKSKRK 80
[Close]
BLAST of Short coiled-coil protein homolog vs. Planmine SMEST
Match: SMESG000029570.1 (SMESG000029570.1)

HSP 1 Score: 230.72 bits (587), Expect = 2.988e-78
Identity = 115/116 (99.14%), Postives = 116/116 (100.00%), Query Frame = 3 
Query:   87 METIPLSSDESDYDKPKNEIKMGSLNFSPDNQLINASTDNKNHMEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRRG 434
            METIPLSSDESDYDKPKNEIKMGSLNFSPDNQLINASTDNKNH+EISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRRG
Sbjct:    1 METIPLSSDESDYDKPKNEIKMGSLNFSPDNQLINASTDNKNHVEISDSIQEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMSASSVFQTNSPKTNRRG 116
The following BLAST results are available for this feature:
BLAST of Short coiled-coil protein homolog vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
SCOC2.468e-2660.76short coiled-coil protein [Source:HGNC Symbol;Acc:... [more]
SCOC2.468e-2660.76short coiled-coil protein [Source:HGNC Symbol;Acc:... [more]
SCOC2.468e-2660.76short coiled-coil protein [Source:HGNC Symbol;Acc:... [more]
SCOC2.468e-2660.76short coiled-coil protein [Source:HGNC Symbol;Acc:... [more]
SCOC2.468e-2660.76short coiled-coil protein [Source:HGNC Symbol;Acc:... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 3
Match NameE-valueIdentityDescription
unc-691.265e-1465.96Short coiled-coil domain-containing protein UNC-69... [more]
unc-692.016e-1465.96Short coiled-coil domain-containing protein UNC-69... [more]
unc-694.825e-1465.96Short coiled-coil domain-containing protein UNC-69... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
CG59343.107e-2456.98gene:FBgn0039505 transcript:FBtr0085158[more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 4
Match NameE-valueIdentityDescription
scoca1.017e-2569.23short coiled-coil protein a [Source:NCBI gene;Acc:... [more]
scoca1.175e-2569.23short coiled-coil protein a [Source:NCBI gene;Acc:... [more]
scoca1.448e-2569.23short coiled-coil protein a [Source:NCBI gene;Acc:... [more]
scocb8.531e-2566.67short coiled-coil protein b [Source:NCBI gene;Acc:... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
SCOC3.586e-2568.18short coiled-coil protein [Source:NCBI gene;Acc:10... [more]
SCOC5.890e-2568.18short coiled-coil protein [Source:NCBI gene;Acc:10... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 4
Match NameE-valueIdentityDescription
Scoc1.694e-2660.76short coiled-coil protein [Source:MGI Symbol;Acc:M... [more]
Scoc1.694e-2660.76short coiled-coil protein [Source:MGI Symbol;Acc:M... [more]
Scoc2.977e-2658.82short coiled-coil protein [Source:MGI Symbol;Acc:M... [more]
Scoc3.584e-2657.47short coiled-coil protein [Source:MGI Symbol;Acc:M... [more]
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BLAST of Short coiled-coil protein homolog vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q5RJZ6|SCOC_RAT1.306e-2550.47Short coiled-coil protein OS=Rattus norvegicus OX=... [more]
sp|Q78YZ6|SCOC_MOUSE2.507e-2557.47Short coiled-coil protein OS=Mus musculus OX=10090... [more]
sp|Q9UIL1|SCOC_HUMAN7.101e-2550.00Short coiled-coil protein OS=Homo sapiens OX=9606 ... [more]
sp|Q08BG7|SCOCA_DANRE1.093e-2469.23Short coiled-coil protein A OS=Danio rerio OX=7955... [more]
sp|Q5XJK1|SCOCB_DANRE7.076e-2466.67Short coiled-coil protein B OS=Danio rerio OX=7955... [more]
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BLAST of Short coiled-coil protein homolog vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A2D0QNE22.979e-2650.89short coiled-coil protein B-like isoform X1 OS=Ict... [more]
A0A4W5NEB05.270e-2663.75Short coiled-coil protein OS=Hucho hucho OX=62062 ... [more]
A0A5N5PPB67.646e-2656.99Uncharacterized protein OS=Pangasianodon hypophtha... [more]
A0A3P8PIB72.593e-2555.91Short coiled-coil protein a OS=Astatotilapia calli... [more]
A0A3P9BZJ92.593e-2555.91Short coiled-coil protein a OS=Maylandia zebra OX=... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 3
Match NameE-valueIdentityDescription
scoca5.285e-2668.18short coiled-coil protein B-like [Source:NCBI gene... [more]
scocb3.431e-2560.26short coiled-coil protein b [Source:NCBI gene;Acc:... [more]
scocb8.293e-2563.89short coiled-coil protein b [Source:NCBI gene;Acc:... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
scocb4.014e-2767.65short coiled-coil protein b [Source:NCBI gene;Acc:... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of Short coiled-coil protein homolog vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO496021.266e-2160.00Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of Short coiled-coil protein homolog vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
scocb3.364e-2661.54short coiled-coil protein [Source:NCBI gene;Acc:10... [more]
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BLAST of Short coiled-coil protein homolog vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000029570.12.988e-7899.14SMESG000029570.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010576 ID=SMED30010576|Name=Short coiled-coil protein homolog|organism=Schmidtea mediterranea sexual|type=transcript|length=672bp
ATTTAAATAATCTATGTCAACACTACTTTTCAAATAATAATATATTTTGA
AATTTATCAAAATTTTCTTAAAATAATATATAGAATATGGAAACTATACC
ACTGTCATCAGATGAGTCTGATTATGACAAACCAAAAAATGAAATAAAAA
TGGGAAGCTTAAATTTTTCACCAGATAACCAACTTATCAATGCAAGTACT
GACAATAAAAATCATATGGAAATCAGTGACAGTATTCAAGAAAAGCAGAA
GTTAATTTCTCAAATTATCGAACTTCAACATACTTTAGAAGATTTGTCTA
GCCGGGTAGAAGTAGTAAAAGAAGAAAATATGAAATTGCGTTCAGAAAAT
CAAATTTTAAGTCAATACAATGAGAATCTCATGAGTGCTAGTTCGGTTTT
TCAGACAAATAGTCCGAAAACTAACAGAAGAGGTTGAATATTTAGATTTC
AACCTCAAAACGGACTATTAGAAATTTCGACTATATTTATGAGAGCTATT
TGATTTGTTGTATATTTAGTTTTTTTGAAACATTTTGTTTATTTACACAT
GTTTAGTTATGATCTGCCATCGTAATAGTGTCTCAAATGTTGCAATATTG
CCCAATTCGGGGTACAAATTTTATTTTTATCATCTTTATTGTACAATATA
AATTTATTGGTTTGAAAAAAAA
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protein sequence of SMED30010576-orf-1

>SMED30010576-orf-1 ID=SMED30010576-orf-1|Name=SMED30010576-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=117bp
METIPLSSDESDYDKPKNEIKMGSLNFSPDNQLINASTDNKNHMEISDSI
QEKQKLISQIIELQHTLEDLSSRVEVVKEENMKLRSENQILSQYNENLMS
ASSVFQTNSPKTNRRG*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000039gamma neoblast
PLANA:0000067goblet cell
PLANA:0000070intestinal phagocyte
Vocabulary: INTERPRO
TermDefinition
IPR019357SCOC
Vocabulary: cellular component
TermDefinition
GO:0000139Golgi membrane
GO:0005737cytoplasm
GO:0005794Golgi apparatus
GO:0005829cytosol
GO:0016020membrane
Vocabulary: molecular function
TermDefinition
GO:0003674molecular_function
Vocabulary: biological process
TermDefinition
GO:0016239positive regulation of macroautophagy
GO:0061635regulation of protein complex stability
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 53..97
NoneNo IPR availableGENE3DG3DSA:1.20.5.170coord: 20..115
e-value: 1.2E-29
score: 103.8
IPR019357Short coiled-coil proteinPFAMPF10224DUF2205coord: 48..109
e-value: 7.6E-23
score: 80.3
IPR019357Short coiled-coil proteinPANTHERPTHR21614SHORT COILED COIL PROTEINcoord: 5..114