SMED30010562

Overview
NameSMED30010562
Smed IDSMED30010562
Length (bp)1974
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010562 (SMED30010562) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010562 (SMED30010562) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010562 (SMED30010562) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010562 (SMED30010562) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010562

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30010562SMESG000070014.1 dd_Smed_v4_1862_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010562SMESG000070014.1 dd_Smed_v4_1862_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010562SMESG000070014.1 Contig42398newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010562SMESG000070014.1 Contig42398uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
parenchymal cellSMED30010562SMESG000070014.1 dd_Smed_v6_1862_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
glial cellSMED30010562SMESG000070014.1 dd_Smed_v6_1862_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010562 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.024663:3566..10400 +9379
Homology
[Close]
BLAST of SMED30010562 vs. Planmine SMEST
Match: SMESG000070014.1 (SMESG000070014.1)

HSP 1 Score: 998.808 bits (2581), Expect = 0.000e+0
Identity = 547/587 (93.19%), Postives = 553/587 (94.21%), Query Frame = 1 
Query:   37 MQTFQFFAAVILCGLVGYHVESLTAKALSKFPLLMNETFHFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELXXXXXXXXXXXXFQPKSNFYIIEIYYVCPQXXXXXXXXLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQNENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQHIENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENVILKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELEKFASKFLSTFEIQLGQMQXXXXXXXXXXXXXTAMIKEIINKMKESDSEKIGKGYXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQSDDFDDNDK 1797
            MQTFQFFA VILCGLVG+HVESLTAKALSKFP LMNETF FQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERM KFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQN+NKEIDCSVVGGFPTPSIKWALTSGPQ+ASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQ+IENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENV LKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELE+FASKFLSTFEIQLGQMQKLLNEKKLEINNNTAMIKEIINKMKESDSEKIGKGY                             +RRKWRFRNRQRPNQSDDFDDNDK
Sbjct:    1 MQTFQFFAVVILCGLVGFHVESLTAKALSKFPFLMNETFQFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMIKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQNKNKEIDCSVVGGFPTPSIKWALTSGPQDASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQNIENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENVNLKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELERFASKFLSTFEIQLGQMQKLLNEKKLEINNNTAMIKEIINKMKESDSEKIGKGY----------------------------WKRRKWRFRNRQRPNQSDDFDDNDK 559
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BLAST of SMED30010562 vs. Planmine SMEST
Match: SMESG000070014.1 (SMESG000070014.1)

HSP 1 Score: 996.882 bits (2576), Expect = 0.000e+0
Identity = 524/534 (98.13%), Postives = 529/534 (99.06%), Query Frame = 1 
Query:   37 MQTFQFFAAVILCGLVGYHVESLTAKALSKFPLLMNETFHFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELXXXXXXXXXXXXFQPKSNFYIIEIYYVCPQXXXXXXXXLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQNENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQHIENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENVILKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELEKFASKFLSTFEIQLGQMQXXXXXXXXXXXXXTAMIKEIINKMKESDSEKIGKGY 1638
            MQTFQFFA VILCGLVG+HVESLTAKALSKFP LMNETF FQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERM KFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQN+NKEIDCSVVGGFPTPSIKWALTSGPQ+ASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQ+IENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENV LKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELE+FASKFLSTFEIQLGQMQKLLNEKKLEINNNTAMIKEIINKMKESDSEKIGKGY
Sbjct:    1 MQTFQFFAVVILCGLVGFHVESLTAKALSKFPFLMNETFQFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMIKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQNKNKEIDCSVVGGFPTPSIKWALTSGPQDASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTDCANKKITNLEHLSENFQKSLQNIENIQTKNEKNTDNLRMEIKQELFQISSLTLNLVKELKQENVNLKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDLVKDQIKEIHGKLNHRNADTGQNQSSFKELERFASKFLSTFEIQLGQMQKLLNEKKLEINNNTAMIKEIINKMKESDSEKIGKGY 534
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BLAST of SMED30010562 vs. Planmine SMEST
Match: SMESG000069999.1 (SMESG000069999.1)

HSP 1 Score: 221.09 bits (562), Expect = 1.908e-63
Identity = 122/348 (35.06%), Postives = 196/348 (56.32%), Query Frame = 1 
Query:   94 VESLTAKALSKFPLLMNETFHFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELXXXXXXXXXXXXFQPKSNFYIIEIYYVCPQXXXXXXXXLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQN-----ENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKIS-CNTDCANKKITNLEH 1119
            + + + K   K  L +N  F  QI P   +  + + + DTM+IQI D  GL+K++ IF+YG  + ++ E  G +M I +WE+N N S+I++ IIF   SNF++ EIY++   SK ++KK +K+L LA RI++ +I L  P  +    +        W +NDT+   CRA+ G+P  IP FEN++ F+ I+S+F  E+NERM    V  E+H+  ++C AG+  K   IKV H P +++IK        N    C    GFPTP+++W L  GP N S +Y +  + + LFI +  P +SKW+F+C+++  + +      +  S   E   I  CN DC + ++   E+
Sbjct:   22 IATFSVKTSYKSQLTLNSIFEIQIQPNLSEPTVAILVGDTMLIQITDNIGLIKQREIFYYGIKDTIENEIKGDYMNIKRWEVNYNDSSINLFIIFHKNSNFHLFEIYFIDAPSKEILKKKIKNLRLAKRIYKRTIELFHPPTLSFTVNSVLGAHSQWEINDTIIAICRAFDGIPNEIPKFENYQGFSNISSTFSLERNERMINMTVGTENHMNPLQCRAGSEVKVHTIKVFHHPTELFIKPKLKLVYSNTTFQCYAKDGFPTPTMEWFLVEGPANHSNYYELHGNRSYLFIKDNCPNHSKWKFKCKLTHMIRNSSRTLVKMISFFFENNTIGQCNLDCLHVRLLEAEY 369
[Close]
BLAST of SMED30010562 vs. Planmine SMEST
Match: SMESG000070072.1 (SMESG000070072.1)

HSP 1 Score: 183.341 bits (464), Expect = 3.280e-50
Identity = 115/348 (33.05%), Postives = 172/348 (49.43%), Query Frame = 1 
Query:  103 LTAKALSKFPLLMNETFHFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELXXXXXXXXXXXXFQPKSNFYIIEIYYVCPQXXXXXXXXLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQ-----NENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFS-----TVVEKPKISCNTDCANKKITNLE 1116
            L  K++SKFP L+ + F   I  +T+ G L+    DTM I+IVD+     E+A   YG    +D EF G  M +  WE N   +A++ISIIF   ++   +EI+Y    +    KK LK+L+LA R+F   I+L  PS + L   P + +     +ND V  +C+A+GG+P+  P FE+     +    +    NE          DHL    C AGN++++  I++ H P  +YI+        N  I C    G+P P I W L  GP NA+ FYNI   G +++IN  S   SKW F C     + SF+ +T +  +      +   P   C   C N +I  +E
Sbjct:   22 LFVKSVSKFPHLIGDAFSLDIQSRTENGKLSSDPSDTMAIRIVDLNFTEIERAFLVYGVLNFMDDEFKGKLMDVKVWEKNPTLTAMNISIIFNRAAHNKYLEIFYFSASNALERKKYLKNLSLAKRLFHELIDLFIPSTLTLSVLPIKVDRREIIINDMVMASCKAFGGIPQEKPRFEDHAHLNVTEIIYNKTANEIRMNLRASLTDHLTNFSCVAGNVTETFTIRIHHHPTALYIQPIKKRFQSNSTIKCYTNDGYPDPEINWQLIEGPPNANLFYNIPPKGDQIYINSNSQTGSKWTFRCT----MKSFLVNTNKILNKTISFAITRNPMEICGEKCLNSRIFEIE 365
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BLAST of SMED30010562 vs. Planmine SMEST
Match: SMESG000014496.1 (SMESG000014496.1)

HSP 1 Score: 180.259 bits (456), Expect = 9.448e-49
Identity = 118/358 (32.96%), Postives = 177/358 (49.44%), Query Frame = 1 
Query:   61 AVILC---GLVGYHVESL-TAKALSKFPLLMNETFHFQILPKTKKGNLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWELXXXXXXXXXXXXFQPKSNFYIIEIYYVCPQXXXXXXXXLKDLNLAYRIFRNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFENFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVKHRPLQVYIKQ------NENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSGTRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEK-PKISCNTDCANKK 1101
            AVI C    LV    + L T   +S  P L+  TF  QI P+     L+    DTMVI+I       +EK+I +YG  + +D+E  G FM I  WE N ++  I+ISI+F+  ++   +EI+Y    +K   +K LK+L+LA R+    I L  PS+++L   P   +     +ND V  +CRA+GG+P+  P FE+     ++   +    NE          DHL    C AGN++++  I++ H P  +YI+         N  I C    G+P P I W L  GP NA +FYN    G ++ IN  SP+ SKW F+C +   L +      +T S  + K P   C   C + +
Sbjct:    5 AVIFCLIQQLVLTDAQPLLTVCFVSTVPHLLKNTFDIQIKPQANGTKLSDDPSDTMVIRIYSFNFTEEEKSILNYGSQDAIDEEILGKFMDIKVWEKNFSSLRINISIMFKRGAHNKYLEIFYFSAPNKFQRRKYLKNLSLAKRLLYQKIELFMPSQLNLSMYPLNVDRKEIIINDMVMASCRAFGGIPQEKPKFEDHVHLNVMEKIYNKTTNEIRMNLRASLSDHLTNFSCSAGNVTETYTIRIHHHPTALYIQPIIKKRFRSNSTIICFTKDGYPDPEINWQLIEGPPNADRFYNFPGKGDQIHINSNSPVGSKWTFKCTMKSMLVNTKKMLNKTISFAIAKNPMEICGEKCLSSR 362
The following BLAST results are available for this feature:
BLAST of SMED30010562 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010562 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000070014.10.000e+093.19SMESG000070014.1[more]
SMESG000070014.10.000e+098.13SMESG000070014.1[more]
SMESG000069999.11.908e-6335.06SMESG000069999.1[more]
SMESG000070072.13.280e-5033.05SMESG000070072.1[more]
SMESG000014496.19.448e-4932.96SMESG000014496.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010562 ID=SMED30010562|Name=SMED30010562|organism=Schmidtea mediterranea sexual|type=transcript|length=1974bp
CTTAAATGAATTAATAAAATTCCCACTTGTCCAGCTATGCAGACTTTTCA
ATTTTTCGCCGCGGTAATTTTGTGTGGATTAGTCGGTTACCATGTCGAGT
CACTTACTGCAAAAGCCTTGTCCAAATTTCCTTTGCTAATGAACGAAACC
TTTCACTTTCAAATCCTTCCGAAAACAAAAAAAGGAAATCTCACGGTTTC
CCTCTTGGATACAATGGTCATACAGATTGTAGACATGGCTGGGTTGGTTA
AGGAAAAGGCGATTTTCCATTATGGGTTTCATGAGAAATTAGATCAAGAG
TTTCATGGGAGTTTCATGAAAATAGTCAAATGGGAACTTAACTCCAATGC
GTCTGCCATTGATATTTCTATAATATTTCAACCGAAAAGTAACTTTTACA
TAATAGAAATTTACTACGTCTGTCCACAATCAAAGTCAATGATGAAAAAA
AGTTTGAAAGATCTCAATTTAGCCTACAGAATTTTTCGAAATTCTATTAA
TCTGTTGAGTCCTTCGAAAGTGGATTTGCGTGCCAGTCCAAATCAACAGA
ATGATTTCATTTGGAACTTGAACGATACCGTTACGGTAACTTGTCGGGCG
TATGGTGGGATGCCGAAAAGAATACCGCTTTTTGAAAACTTCAAATCCTT
CGCCATCATTAATAGCTCTTTTTTCCCTGAAAAAAATGAGCGAATGTTTA
AATTTTTAGTGCGCAGAGAGGATCACCTTAAAACAATAAAATGCGGCGCT
GGAAATATTTCCAAGTCTCAGATCATCAAAGTGAAACACCGCCCATTGCA
AGTTTATATAAAACAAAACGAAAACAAAGAAATTGATTGCTCTGTTGTGG
GTGGTTTCCCGACACCTAGCATCAAATGGGCACTCACTTCCGGACCTCAA
AATGCGTCAAAATTCTATAATATCACTTCAAGTGGAACTCGGCTTTTCAT
AAATGAAAAGAGCCCTCTCAATTCAAAATGGGAATTTGAATGTCAAATCA
GTCAAAGACTTGGATCATTTATAACGCATACGAAACGAACATTTTCAACT
GTTGTAGAAAAACCGAAAATTTCTTGCAATACTGATTGTGCTAATAAGAA
AATTACAAATCTTGAACATTTATCAGAAAATTTTCAAAAATCACTTCAAC
ATATTGAAAACATTCAAACAAAAAACGAAAAAAACACCGATAATCTTCGA
ATGGAAATAAAACAAGAATTATTTCAAATTTCTAGTTTGACCTTAAATTT
AGTAAAAGAATTAAAACAAGAAAATGTAATTTTAAAACAAATCCAAAAAT
CTGACATCAATTCATTAAAAGGAGATTTGGCAGAAATCCGACAAAAAATG
AAAGAGAAATTCATCAACAAAACTGAAATAGATTTAGTAAAAGATCAAAT
CAAAGAAATCCATGGGAAATTGAATCATAGAAATGCGGATACCGGCCAGA
ACCAGAGCAGTTTTAAAGAACTCGAAAAGTTTGCCTCCAAGTTCCTAAGT
ACCTTTGAAATTCAACTGGGGCAAATGCAAAAATTACTAAATGAAAAGAA
ATTGGAAATTAATAACAACACAGCCATGATAAAAGAAATAATCAACAAAA
TGAAAGAAAGCGATAGTGAAAAAATTGGTAAAGGATATTGGAAAAGACGA
AAATGGAGATTCAGAAACCGACAAAGGCCTAATCAAAGACGAAAATGGAG
ATTCAGAAACCGACAAAGGCCTAATCAAAGACGAAAATGGAGATTCAGAA
ACCGACAAAGGCCTAATCAAAGTGATGATTTTGATGACAATGATAAATAA
TGTGCTGTAAAATTTTTTCATTAAAAAAAAGTTGTATTTTTAAGTTATCA
ATATCAAATTGAAATGCATATATGATTTTCATCTATTAGAGTGACCTTGA
AATTTTATAGTGTTAAGTTGTCATTTTGCAGTGTAGATTTCAATTTAAGG
TATGTTTTTTTGATTAGGGATATC
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protein sequence of SMED30010562-orf-1

>SMED30010562-orf-1 ID=SMED30010562-orf-1|Name=SMED30010562-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=588bp
MQTFQFFAAVILCGLVGYHVESLTAKALSKFPLLMNETFHFQILPKTKKG
NLTVSLLDTMVIQIVDMAGLVKEKAIFHYGFHEKLDQEFHGSFMKIVKWE
LNSNASAIDISIIFQPKSNFYIIEIYYVCPQSKSMMKKSLKDLNLAYRIF
RNSINLLSPSKVDLRASPNQQNDFIWNLNDTVTVTCRAYGGMPKRIPLFE
NFKSFAIINSSFFPEKNERMFKFLVRREDHLKTIKCGAGNISKSQIIKVK
HRPLQVYIKQNENKEIDCSVVGGFPTPSIKWALTSGPQNASKFYNITSSG
TRLFINEKSPLNSKWEFECQISQRLGSFITHTKRTFSTVVEKPKISCNTD
CANKKITNLEHLSENFQKSLQHIENIQTKNEKNTDNLRMEIKQELFQISS
LTLNLVKELKQENVILKQIQKSDINSLKGDLAEIRQKMKEKFINKTEIDL
VKDQIKEIHGKLNHRNADTGQNQSSFKELEKFASKFLSTFEIQLGQMQKL
LNEKKLEINNNTAMIKEIINKMKESDSEKIGKGYWKRRKWRFRNRQRPNQ
RRKWRFRNRQRPNQRRKWRFRNRQRPNQSDDFDDNDK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0003116parenchymal cell
PLANA:0007528glial cell
Vocabulary: INTERPRO
TermDefinition
IPR036179Ig-like_dom_sf
IPR013783Ig-like_fold
IPR007110Ig-like_dom
Vocabulary: molecular function
TermDefinition
GO:0005515protein binding
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 356..379
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..570
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 550..568
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..18
score: 0.632
NoneNo IPR availableTMHMMTMhelixcoord: 7..25
IPR013783Immunoglobulin-like foldGENE3DG3DSA:2.60.40.10coord: 247..345
e-value: 1.1E-5
score: 27.3
IPR007110Immunoglobulin-like domainPROSITEPS50835IG_LIKEcoord: 244..337
score: 8.121
IPR036179Immunoglobulin-like domain superfamilySUPERFAMILYSSF48726Immunoglobulincoord: 252..331