G_PROTEIN_RECEP_F1_2 domain-containing protein
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010542 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 7
Homology
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Match: gpr139 (G protein-coupled receptor 139 [Source:NCBI gene;Acc:798482]) HSP 1 Score: 54.6842 bits (130), Expect = 1.391e-7 Identity = 73/313 (23.32%), Postives = 138/313 (44.09%), Query Frame = 3 Query: 423 IGICVILATIVIIKSKCLGKFTNNY-YLIALGIIDVCSLITILLISPPAQRWIPFLEN--MKELMPPYLSTILQM----TVFISNWITVALALDRHQAICKPFNYYSQSYTLHNKTCALILIIIGCVLLYTPQXXXXXXXXXXXXTEVLSFLKYTSLGMNTFYSNFH-LWFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASFTKENIKICVLLSCLITSFVFLQIP--FVILIFLINYRPSMFNGNK-IQLYSQIVTFLYVLKHDINFLFYLWLSKRFMR----TIQAFVCFNCNN 1316 +G+ + T++I+ L + ++Y YL+AL + D+ L+ I+ + LE+ + +P L+ +Q+ ++ S WITV L +DR+ A+C P Y++ SY + ++ + GC++ P + E+ L S G + + H L +V+ + LLP + +N +++ R ++ R S K+ +L + + F L P +IL L +P+M + + L + + L +L +NF Y ++SKRF R ++AF F C Sbjct: 38 LGLPANILTVIILSQLVLRRQKSSYNYLLALAVADILVLLLIVFVDF-------LLEDFILGAPLPHSLNKAVQVLEFSSIHTSIWITVPLTIDRYIAVCHPLRYHTVSYPARTRK-VILAVYAGCLISSVP---------YYWWPELWHGLPGASPGGRSSSAGQHVLVWVHCATVYLLPCSVFFSLNA-IIVRKLRCRRSCFRLRGYSTGKTT------------AILLAITSVFAVLWAPRTLMILYHLYTVQPAMPGPARLLHLVTDVANMLALLNTGVNFFLYCFISKRFRRMAGTVLKAF--FRCRK 318
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A0L8FQN5 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Octopus bimaculoides OX=37653 GN=OCBIM_22010749mg PE=4 SV=1) HSP 1 Score: 92.0485 bits (227), Expect = 2.025e-16 Identity = 86/318 (27.04%), Postives = 147/318 (46.23%), Query Frame = 3 Query: 387 LFVSIITPWSCAIGICVILATIVIIKSKCLGKFTNNYYLIALGIIDVCSLITILLISPPAQRWIPFLE----NMKELMPPYLSTILQMTVFISNWITVALALDRHQAICKPFNYYSQSYTLHNKTCALILIIIGCVLLYTPQXXXXXXXXXXXX-TEVLSFLKYTSLGMNTFYSNFHLWFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASF--TKENIKICVLLSCLITSFVFLQIPFVILIFLINYRPSMFNGNKIQLYSQIVTFLYVLKHDINFLFYLWLSKRFMRTIQAFVCFNCNNI 1319 + V ++ P +C GI I+ T++I+ K + TN Y L L D+ LI + L + W + N E+ Y L+M +S W+TV LA++R AIC P+ T++ C ++ I I + +P F + S N E L T L ++ + W + + +++P +IL++N+ L+ + ST++L + I + + SF + E +KI +L + F+ Q P+V+ +LI+ + L I L LK INF+ Y W S+RF T + +CF C + Sbjct: 20 VLVEVVLPSACICGIVGIITTLIILSHKHMATSTNTY-LTGLAAADLMFLIIVSL-----RLWGVMVSHSDLNTWEIFTAYSEIFLRMFNLVSVWMTVMLAVERFIAICIPYKAMDMC-TIYRSRCIVVFIYIAAFVCQSPHFAKYKIVTSHNEFNETEYTLHQTLLSGEILFTIIYGWVLEGLSGVIVPFVLILVLNSRLIYEIHKSTQYLKR----QIGANTRTSFDISSEQMKITTMLIAMCVVFLICQAPYVLYNYLISIQAGSLRSRNHLLIMAISILLAALKSSINFVLYCWFSERFWTTFKTIMCFRCARL 326
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: A0A210PPP6 (FMRFamide receptor OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT25244 PE=4 SV=1) HSP 1 Score: 71.2478 bits (173), Expect = 1.842e-9 Identity = 84/327 (25.69%), Postives = 150/327 (45.87%), Query Frame = 3 Query: 348 NNGT----FEYSPWINTLFVSIITPWSCAIGICVILATIVIIKSKCLGKFTNNYYLIALGIIDVCSLITILLISPPAQRWIPFLENMKELMPPYLSTILQMTVFISNWITVALALDRHQAICKPFNYYSQSYTLHNKTCALILIIIGCVLLYTPQXXXXXXXXXXXXTEVLSFLKY-----TSLGMNTFYSNFHLWFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASFTKENIKICVLLSCLITSFVFLQIPFVILIFLINYRP----SMFNGNKIQLYSQIVTFLYVLKHDINFLFYLWLSKRFMRTIQ 1289 NGT SP N LF ++ P C GI I+ T++++ K + TN Y L +L I D+ + IL R + ++ ++ Y L + S W+TV LA++R+ AIC P ++ ++ A I+I++ V + + F T + ++ T LG N Y + W V+ I+ +LP +L +N L+I + ST++L +L +++ + + E +KI ++L +I F Q P+VI + N ++ +++ + F+ LK INF+ Y W S++F T + Sbjct: 42 KNGTTSSMLRSSPSYNLLF-KVLLPIICVSGIVGIILTVMVLSRKNMCTSTNCY-LTSLSIADL-GFLVILSTKFLEYRITGYPFHVFDIYFTYAQIFLHTFLLASVWLTVMLAIERYIAICHPL----RAISICTVRRARIIIVVIFVFSFYCRVPNFFEQEIKTVTPCPGYPEFRHMVLTKLGQNLTYKISYAWVVDCIICAILPFIALLFLNIRLIIEIRKSTKYLRY--HLGTDSNMQNAVSNEQLKITMMLISIIFVFFVCQAPYVITMAYRNIHQYTSRTILRSLTMEIVADATIFMLALKSAINFILYCWFSEKFWNTFK 359
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Match: R7TMC4 (G_PROTEIN_RECEP_F1_2 domain-containing protein OS=Capitella teleta OX=283909 GN=CAPTEDRAFT_215199 PE=4 SV=1) HSP 1 Score: 67.3958 bits (163), Expect = 2.693e-8 Identity = 70/239 (29.29%), Postives = 118/239 (49.37%), Query Frame = 3 Query: 636 MTVFI--SNWITVALALDRHQAICKPFNYYSQSYTLHNKTCA-------LILIIIGCVLLYTPQ--XXXXXXXXXXXXTEVLSFLKYTSLGMNTFYSNFHLWFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASFT--KENIKICVLLSCLITSFVFLQIPFVI--LIFLINYRPSMFNGNKIQ--LYSQIVTFLYVLKHDINFLFYLWLSKRFMRTIQAFVC 1301 M VF+ S W+TV LA++R+ AIC+PF L K C+ ++ I + L+ P F + +S+ K T L N Y + W V+ IL+ +LP ++L++N L+ V STR+L S+S +++ T KE ++I V+L +I F Q P+V+ IF IN + + +G+ ++ Q+ + K +NF+ Y W S++F T++ C Sbjct: 114 MHVFLMASIWLTVMLAVERYIAICQPF--------LAAKVCSVKRARIFVVSIYVFAFLVRLPAFWEHEVVTRFDTLSNTSISYTKSTELASNVHYLRINPWVVDGILMSILPFILLLVLNVRLIWEVRKSTRYLQATMVASVSAPGRSAGTIKKEELQITVMLISIIIVFFICQAPYVVYTAIFSIN-KDILSSGSSTGFLIFHQLAIVVLAFKSAVNFIIYCWFSEKFWATLKKIFC 343
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000039899.1 (SMESG000039899.1) HSP 1 Score: 65.855 bits (159), Expect = 1.934e-11 Identity = 75/319 (23.51%), Postives = 145/319 (45.45%), Query Frame = 3 Query: 381 NTLFVSIITPWSCAIGICVILATIVIIKSKCLGKFTNNYYLIALGIIDVCSLITILLISPPAQRWIPFLENMKELMP------PYLSTILQMTVFISNWITVALALDRHQAICKPFNYYSQSYTLHNKTCALILIIIGCVLLYTPQXXXXXXXXXXXXTEVLSFLKYTSLGMNTFYSNFHL---WFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASFTKENIKICVLLSCLITSFVFLQIPFVILIFLIN---YRPSMFNGNKIQLYSQIVTFLYVLKHDINFLFYLWLSKRFMRTIQAFVC 1301 N + + +TP I I VI+ T++II + L + Y+ ALG++D+ L+ + + S F+ N MP Y+ + + + +SNW+T L ++R+ + PF ++ T ++ + +G VL+ P + NY S + F ++T + + NF L W V+ L L++P +++ VN L++ + S+RF+ Q+ + E KI ++ C+I +F Q PF+ L N + NG + ++ + +K D +F+ + W+ +F ++ C Sbjct: 26 NRVIKNYLTPLMSVISITVIVITLLIIHKRNLW-ISTRIYITALGLVDILGLVILTIASLDY-----FVLNYTINMPIFYRIYQYIFILFEAVLLLSNWLTTILGIERYLVVSYPF--LTKRITNSHRKLITLFCFVGTVLVKIPSLILLINYESIVNND---FQQHTQEIEKSLH-NFELWQSWIVDFSLFLIVPFCVLIFVNARLILTIRRSSRFMRQY-----CANRHRKIENEERKITCIMICVIMAFFICQGPFITYTALSNVHLFHQDYKNGQ--SMLQELSILILSMKSDFSFVLHCWVCDKFGAALKRMFC 325
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Match: SMESG000078268.1 (SMESG000078268.1) HSP 1 Score: 55.8398 bits (133), Expect = 3.495e-8 Identity = 77/299 (25.75%), Postives = 136/299 (45.48%), Query Frame = 3 Query: 444 ATIVIIKSKCLGKFTNNYYLIALGIIDVCSLITILL--ISPP-----------AQRWIPFLENMKELMPPYLSTILQMTVFISNWITVALALDRHQAICKPFNYYSQSYTLHNKTCALILIIIGCVLLYTPQXXXXXXXXXXXXTEVLSFLKYTSLGMNTFYSNFHLWFVNXXXXXXXXXXXXXMVNTHLVICVTSSTRFLYQFRNLSISKSVKASFTKENIKICVLLSCLITSFVFLQIPFVILIFLINYRPSMFNGNKIQLYSQIVTFLYVLKHDINFLFYLWLSKRFMRTIQAFVC 1301 TIVI K + + ++ +GI+D+ +L+ ++ I P +W N+K ++ +L + +FISNWITV L +R+ A+C P YS+ ++ A+ +I + ++ P Y L+ + Y++ LW + L L L++P + ++N L++ V S+RFL Q N + + S T+E+ VL+ ++ FVF Q PF I + L FN + I L I + L LK ++F Y W ++F + + +C Sbjct: 40 GTIVIALKKGIW-LSTRMFMTIMGIVDLFALVLFIIFNIEKPLIELELLNFEMISKWF----NVKMILAEFLHGL----IFISNWITVLLGFERYIAVCYPL--YSRRIDYIHRKIAVAVIFVVIIIFKLP-------LIIMIYLHRFHDLRIQRNFIYKLYTHVQLWIITLFLFLIVPFVCLALINFQLILAVRRSSRFLRQCSNQNPNYQNSNSRTEEHKITFVLIGIIVIFFVF-QGPFGIYVTL-----QSFNYHSISLRG-ISSILLSLKSIVHFFLYCWFCEKFRQILYHLLC 313 The following BLAST results are available for this feature:
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 1
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 3
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of G_PROTEIN_RECEP_F1_2 domain-containing protein vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 2
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010542 ID=SMED30010542|Name=G_PROTEIN_RECEP_F1_2 domain-containing protein|organism=Schmidtea mediterranea sexual|type=transcript|length=1604bpback to top protein sequence of SMED30010542-orf-1 >SMED30010542-orf-1 ID=SMED30010542-orf-1|Name=SMED30010542-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=381bp MNSSAKKWRLICNTANELYIVLDCDRKDFNINLLMNNGTFEYSPWINTLFback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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