SMED30010499
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010499 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 33Note: Hover over icons to view figure legend
Alignments
SMED30010499 aligns in the following genomic locations:
Homology
BLAST of SMED30010499 vs. Ensembl Human
Match: HSPG2 (heparan sulfate proteoglycan 2 [Source:HGNC Symbol;Acc:HGNC:5273]) HSP 1 Score: 206.453 bits (524), Expect = 1.559e-59 Identity = 173/620 (27.90%), Postives = 257/620 (41.45%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMF---NNAAMFVSMCLKCFCSGQTDDCSSAMGY-------YQSPNPKTSTRTKGLLMNFKSQ--------KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQG---AIVCKCTGRERNDPLCDKC 8040
C + ACRN CI + C+G +DCEDG DE + CEPN+F C + C WRCDG+ DC + DE NC K + C + F C IP S CDE +C + DE C P+VV P +E+ + ++ C + VPT INWR NWG +P TV G L I ++ G YTCEA++ + + V + C F ++AA CL CFC G T C S + + P+ KG+ + +Q L++ + + + S +F+ ++ F P FL G+ ++SY L+ N+R L + + P V+L + L S G NQ ++ E E+W +S R ++L VL ++ +LI+ Y + + +D +T+ S G A +E+C CP Y+GLSCE C A + + + C NC CD G+C C GP+C +CK GF + ++ C C C + S+ C D GQ A TGR C+ C
Sbjct: 285 CGPQEAACRNGHCIPRDYLCDGQEDCEDGSDELDCGPPPPCEPNEFPCG-------NGHCALKLWRCDGDFDCEDRTDEANCPTKRPEEVCGPTQFRCVSTNMCIPASFHCDEESDCPDRSDEFGCMPPQVVTPPRESIQASRGQT-VTFTCVAIGVPTPIINWRLNWGHIPSHPRVTVTSEGG-----RGTLIIRDVKESDQGAYTCEAMNARGMVFGIPDGVLELVPQRGPCPDGHFYLEHSAA-----CLPCFCFGITSVCQSTRRFRDQIRLRFDQPDD-----FKGVNVTMPAQPGTPPLSSTQLQIDPSLHEFQLVDLSRRFL--VHDSFWALPEQFL-------GNKVDSYGGSLRYNVRYELARGMLEPVQRPDVVLMGAGYRLL-------SRGHTPTQPGALNQRQVQFSE-EHWVHESG--RPVQRAELLQVLQSLEAVLIQTVYNTKMASVGLSDIAMDTTVTHATSHGR---AHSVEECRCPIGYSGLSCESCDAHFTRVPGGPYLGTCSGCNCNGHASSCDPVYGHCLNCQHNTEGPQCNKCKAGFFGDAMKATATSCRPCPCPYIDASRRFSDTCFLDTDGQATCDACAPGYTGRR-----CESC 854 HSP 2 Score: 47.3654 bits (111), Expect = 1.559e-59 Identity = 21/62 (33.87%), Postives = 29/62 (46.77%), Query Frame = -3
Query: 6039 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6212
C+ G+ C+CK N G C+EC+ G F + CLKCFC ++ C S R
Sbjct: 879 CDERGSMGTSGEACRCKNNVVGRLCNECADGSFHLSTRNPDGCLKCFCMGVSRHCTSSSWSR 940 HSP 3 Score: 76.2554 bits (186), Expect = 7.342e-21 Identity = 84/361 (23.27%), Postives = 133/361 (36.84%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDCSS-----AMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG-------PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQN----QCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
CLKCFC G + C+S A + S P + T + ++ I + + + S ++ + Y F P+ FL G + SY L+ + ++ PG P V+LQ G ++ + E P F + W++ + A R +L L+ I LLIR Y ++D + P+ G + A +E+C+CP Y G SC+ C GY + C + C + C+ + G C C GP+C +C+PG+ DC C CY ++ C D G P CD C
Sbjct: 921 CLKCFCMGVSRHCTSSSWSRAQLHGASEEPGHFSLTNAASTHTTNEGIFSPTPGELGF---SSFHRLLSGPY--FWSLPSRFL-------GDKVTSYGGELRFTV-----TQRSQPGSTPLHGQPLVVLQ---GNNIILEHHVAQEPSPGQPSTFIVPFREQAWQRPD-------GQPATREHLLMALAGIDTLLIRASYAQQPAESRVSGISMD--VAVPEETGQDP-ALEVEQCSCPPGYRGPSCQDCDTGYTRTPSGLYLGTCERCSCHGHSEACEPETGACQGCQHHTEGPRCEQCQPGYYGDAQRGTPQDCQLCPCYGDPAAGQAAHTCFLDTDGH--------------PTCDAC 1237 HSP 4 Score: 47.7506 bits (112), Expect = 7.342e-21 Identity = 23/66 (34.85%), Postives = 31/66 (46.97%), Query Frame = -3
Query: 6039 NCN---SLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6215
NC+ S+ ++ G+C+CK +G CS C P F CL CFC TQ+C S R
Sbjct: 1276 NCDPQGSVSSQCDAAGQCQCKAQVEGLTCSHCRPHHFHLSASNPDGCLPCFCMGITQQCASSAYTR 1341 HSP 5 Score: 67.781 bits (164), Expect = 5.038e-10 Identity = 49/188 (26.06%), Postives = 74/188 (39.36%), Query Frame = -2
Query: 6283 AMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLS---SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6813
A R +L L+D+ LLIR + S +++ P N A +E+C CP Y GLSC+ C GY + + C + EC+ + G C++C +G C C PG+ YG+ + DC C C + S C + G G C C++C
Sbjct: 1476 ATREHLLMALADLDELLIRATFSSVPLAASISAVSLEVAQPGPS---NRPRALEVEECRCPPGYIGLSCQDCAPGYTRTGSGLYLGHCEL--CECNGHSDLCHPETGACSQCQHNAAGEFCELCAPGY---YGDATAGTPEDCQPCACPLTNPENMFSRTC--ESLGAGGYRCTACEPGYTGQYCEQC 1653
BLAST of SMED30010499 vs. Ensembl Human
Match: LAMA1 (laminin subunit alpha 1 [Source:HGNC Symbol;Acc:HGNC:6481]) HSP 1 Score: 76.6406 bits (187), Expect = 5.721e-16 Identity = 87/354 (24.58%), Postives = 143/354 (40.40%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFK---SQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTG-FEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ-------NQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
C +CFC G +D CSS N + L+ K Q L + SI N+ + + Y + P +L + G +L+ Y + +I + + + VI++ NG +L + +E + P + L + EN F D + R ++ VL+++ +LLIR Y S + + + ++D +N + + A +E C CP YTG SCE CL+GY + D C +C + EC+ +G C C +G C +C PGF DC C C + + S C L +VC + C++C
Sbjct: 500 CSECFCFGVSDVCSSLSWPVGQVNSMSGWLVTDLISPRKIPSQQDALGGRHQVSIN--NTAVMQRLAPKY--YWAAPEAYLGNKLTAFGGFLK---YTVSYDIPVETVDSNLMSHADVIIKG-NGLTL----STQAEGLSLQP--YEEYLNVVRLVPEN-FQDFHSKRQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIASSNAIDL---VVAADVEHCECPQGYTGTSCESCLSGYYRVDGILFGGICQPC--ECHGHAAECNVHGVCIACAHNTTGVHCEQCLPGFYGEPSRGTPGDCQPCACPLTIASNNFSPTC--HLNDGDEVVCDWCAPGYSGAWCERC 831 HSP 2 Score: 30.4166 bits (67), Expect = 5.721e-16 Identity = 21/58 (36.21%), Postives = 27/58 (46.55%), Query Frame = -3
Query: 6072 DLSENKNCNSLGTENIVDGKC-KCKENYKGPNCSECSPGFFS---FQKKCLKCFCSNK 6233
D SE +C+S V G+C KC N G +C C+ GF+ K C C C K
Sbjct: 856 DPSEAGHCDS------VTGECLKCLGNTDGAHCERCADGFYGDAVTAKNCRACECHVK 907 HSP 3 Score: 70.0922 bits (170), Expect = 9.770e-11 Identity = 68/280 (24.29%), Postives = 120/280 (42.86%), Query Frame = -3
Query: 5385 GKCKCKENYKGPNCSECSPGFFSFQK----KCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLL-YGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEK------TKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGK----IAPVEMCLCPKGNPGIKCEAC 6179
G C CKEN GP C+EC G F+ + C CFCS + C E + Y + +TL S D L V+S + G E + + + +L Q ++ + +P L+ YGGK+ ++ + + L + S+ P + I K GR+ + + + + + + M +NF K+ K + RE F+ L +++ I + G ++ R+ D+++E + L + +E C+CP G G C+ C
Sbjct: 1116 GACPCKENVFGPQCNECREGTFALRADNPLGCSPCFCSGLSHLCSELEDYVRTPVTLGS-DQPLLRVVSQSNLRGTTEG-------VYYQAPDFLLDAATVRQ----HIRAEPFYWRLPQQFQGDQLMAYGGKLKYSVAFYS--LDGVGTSNFEPQVLI---KGGRIRKQVIYMDAPAPENGVRQEQEVAMRENFWKYFNSVSEKPVT-REDFMSVLSDIEYILIKASYGQG-LQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDC 1376 HSP 4 Score: 67.781 bits (164), Expect = 4.766e-10 Identity = 71/292 (24.32%), Postives = 122/292 (41.78%), Query Frame = -3
Query: 5385 NCNSLGT--ENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGL---VYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESL-----FMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKW-MNDLGKIAPVEMCLCPKGNPGIKCEAC 6215
CN +G+ + G C CKEN +G C C PGF++ ++K C +CFC + C S + + S + D++ S R+I +A +Q+ +N T ++ + P Y+ KLT +GG + +T Y D+ + V S++ ++ K G + + + E + +NF + K DR+ + L HL I +++ + RL ++++ A DL A VE C CP+G G CE+C
Sbjct: 455 GCNPVGSASDEPCTGPCVCKENVEGKACDRCKPGFYNLKEKNPRGCSECFCFGVSDVCS-SLSWPVGQVNSMSGWLVTDLI-SPRKIPSQQDALGGRHQVSINNT--AVMQRLAPKYYWAAPEAYLGNKLT-------------AFGGFLKYTVSY--DIPVETVDSNLMSHADVIIKGNGLTLSTQAEGLSLQPYEEYLNVVRLVPENFQDF----HSKRQIDRDQLMTVLANVTHLLIRANYNSAKMALYRLESVSLDIASSNAIDLVVAADVEHCECPQGYTGTSCESC 723 HSP 5 Score: 63.929 bits (154), Expect = 6.918e-9 Identity = 80/324 (24.69%), Postives = 122/324 (37.65%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVF-LTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSN---PE-GFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP----INCVECDKN----------GNCNRCTGQRSGPKCLECKPGFVRLYGEKL--SSDCTECNC 7311
C CFCSG + CS Y ++P S + L+ SQ L + + +Y + Q +P + L F G L +Y LK ++ + S D V N + L +VIY + PE G + ++++ E + +S E R D ++VLSDI+Y+LI+ Y + +++ A +E C CP G SC+ C GY + + P CV C N G C C +G C C G+ YG+ +SDC C C
Sbjct: 1147 CSPCFCSGLSHLCSELEDYVRTPVTLGSDQP---LLRVVSQSNLRGT-TEGVYYQAPDFLLDAATVRQHIRAEPFYWRLPQQF--QGDQLMAYGGKLKYSVAFY--SLDG-----VGTSNFEPQVLIKGGRIRKQVIYMDAPAPENGVRQEQEVAMRENFWKYFNSVSEKPVTREDFMSVLSDIEYILIKASYGQGLQQSRISDISMEVGRKAEKLHPEEEVASLLENCVCPPGTVGFSCQDCAPGYHRGKLPAGSDRGPRPLVAPCVPCSCNNHSDTCDPNTGKCLNCGDNTAGDHCDVCTSGY---YGKVTGSASDCALCAC 1454
BLAST of SMED30010499 vs. Ensembl Human
Match: LDLR (low density lipoprotein receptor [Source:HGNC Symbol;Acc:HGNC:6547]) HSP 1 Score: 84.7297 bits (208), Expect = 2.429e-15 Identity = 50/130 (38.46%), Postives = 66/130 (50.77%), Query Frame = -2
Query: 7669 VKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8052
V D C N+ C++ CI C+G +C+DG DE TC C+ FSC GG+ +C+ +WRCDG+ DC NG DE C TC F C D I R CD + +CL+G DE +C
Sbjct: 108 VGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSC---GGR--VNRCIPQFWRCDGQVDCDNGSDEQGC---PPKTCSQDEFRCHDG-KCISRQFVCDSDRDCLDGSDEASC 228 HSP 2 Score: 61.2326 bits (147), Expect = 3.470e-8 Identity = 40/132 (30.30%), Postives = 57/132 (43.18%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-------LNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 8040
CS ++ C + CI C+ +DC DG DE C P F C++ C+ W CD + DC +G DE L + D + C F C + S+W CD P+C + DE+NC
Sbjct: 194 CSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNS-------STCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECI--HSSWRCDGGPDCKDKSDEENC 316
BLAST of SMED30010499 vs. Ensembl Human
Match: LDLR (low density lipoprotein receptor [Source:HGNC Symbol;Acc:HGNC:6547]) HSP 1 Score: 84.3445 bits (207), Expect = 3.386e-15 Identity = 50/130 (38.46%), Postives = 66/130 (50.77%), Query Frame = -2
Query: 7669 VKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8052
V D C N+ C++ CI C+G +C+DG DE TC C+ FSC GG+ +C+ +WRCDG+ DC NG DE C TC F C D I R CD + +CL+G DE +C
Sbjct: 23 VGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSC---GGRV--NRCIPQFWRCDGQVDCDNGSDEQGC---PPKTCSQDEFRCHDG-KCISRQFVCDSDRDCLDGSDEASC 143 HSP 2 Score: 60.4622 bits (145), Expect = 5.915e-8 Identity = 40/132 (30.30%), Postives = 57/132 (43.18%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-------LNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 8040
CS ++ C + CI C+ +DC DG DE C P F C++ C+ W CD + DC +G DE L + D + C F C + S+W CD P+C + DE+NC
Sbjct: 109 CSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNS-------STCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECI--HSSWRCDGGPDCKDKSDEENC 231
BLAST of SMED30010499 vs. Ensembl Human
Match: LDLR (low density lipoprotein receptor [Source:HGNC Symbol;Acc:HGNC:6547]) HSP 1 Score: 83.9593 bits (206), Expect = 4.451e-15 Identity = 50/130 (38.46%), Postives = 66/130 (50.77%), Query Frame = -2
Query: 7669 VKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8052
V D C N+ C++ CI C+G +C+DG DE TC C+ FSC GG+ +C+ +WRCDG+ DC NG DE C TC F C D I R CD + +CL+G DE +C
Sbjct: 23 VGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSC---GGRV--NRCIPQFWRCDGQVDCDNGSDEQGC---PPKTCSQDEFRCHDG-KCISRQFVCDSDRDCLDGSDEASC 143 HSP 2 Score: 59.6918 bits (143), Expect = 1.013e-7 Identity = 40/132 (30.30%), Postives = 57/132 (43.18%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-------LNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 8040
CS ++ C + CI C+ +DC DG DE C P F C++ C+ W CD + DC +G DE L + D + C F C + S+W CD P+C + DE+NC
Sbjct: 109 CSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNS-------STCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECI--HSSWRCDGGPDCKDKSDEENC 231
BLAST of SMED30010499 vs. Ensembl Celegans
Match: unc-52 (Basement membrane proteoglycan [Source:UniProtKB/Swiss-Prot;Acc:Q06561]) HSP 1 Score: 171.785 bits (434), Expect = 5.769e-42 Identity = 201/736 (27.31%), Postives = 292/736 (39.67%), Query Frame = -2
Query: 6418 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYY------------------------QSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ G+Y Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSS-GHYRTKVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDG---QQATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 713 HSP 2 Score: 60.4622 bits (145), Expect = 2.890e-8 Identity = 23/51 (45.10%), Postives = 29/51 (56.86%), Query Frame = -3
Query: 6039 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYR 448
BLAST of SMED30010499 vs. Ensembl Celegans
Match: unc-52 (Basement membrane proteoglycan [Source:UniProtKB/Swiss-Prot;Acc:Q06561]) HSP 1 Score: 161.384 bits (407), Expect = 4.184e-39 Identity = 182/706 (25.78%), Postives = 276/706 (39.09%), Query Frame = -2
Query: 6460 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITN--PDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PIN-----------CVECDKNGNCNRCTGQRSGPKCLECKP 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ Y + +G+ ++ ++ ++ + S + ++ + P FL G + +Y ++ I G S P V+L+ ++ N + +P + + +E+ N +A R D+L VL+D+ LIR + ++QT + + + + PD + A +E+C CP Y G SCE C GYE++ + C PI G C +C GP C C P
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAEGVTISDIEERTIDRNTPFSFFKTGYLTFDGTTDGVAKYWRLPQRFL-------GDKVTAYGGKMEFEIEFSG-SGHHSSEPMVVLKGNQNILVHRVRNQEHVLRSDSPVRITVETYETNYEQLNG-------AAATREDLLMVLADLDAFLIRATHVAHQTSTSLGDVSWEIAVDRYTPDGL-----ALEVEQCVCPPGYLGTSCEDCAPGYERSGYGPYLGTCVPIQPRHQQCGPGAVAPTAPAQGQC-QCKASVIGPNCDRCAP 705 HSP 2 Score: 77.411 bits (189), Expect = 1.674e-13 Identity = 88/342 (25.73%), Postives = 132/342 (38.60%), Query Frame = -2
Query: 6418 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS------------AMG-----------YYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 7356
C F A C+ CFCSG T CS+ A G Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 703 CAPNSFGLAPTNPQGCIPCFCSGVTQQCSASSYRRTSVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDGQ---QATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 1002 HSP 3 Score: 54.299 bits (129), Expect = 1.808e-6 Identity = 24/63 (38.10%), Postives = 34/63 (53.97%), Query Frame = -3
Query: 6003 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIK 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR + + + D +
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAE 460 HSP 4 Score: 53.5286 bits (127), Expect = 3.100e-6 Identity = 61/266 (22.93%), Postives = 107/266 (40.23%), Query Frame = -3
Query: 5412 VDGKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEY----NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKG 6185
G+C+CK + GPNC C+P F + C+ CFCS TQ+C S YR ++++++ A D + +++ Q + Q + I + G + KL + D + YGG + +TF++ N+D D++ I ++ K + + K +E+ + + G++ RE + L L I + N D +L +E A+ A VE C+CP G
Sbjct: 685 AQGQCQCKASVIGPNCDRCAPNSFGLAPTNPQGCIPCFCSGVTQQCSAS-SYRRTSVSIDYARGDRDQL----ELTTSDSRQPYSPQTRAELSGQAIEFRSFEEARGQT-LYWKLPEKFLGDKV----TSYGGTLEYTFKFSGNGNSDQSADVILRGND--IALQYKHREPFYADRENKVQIKIIETSWQRVD------GQQAT----REHLLMTLADLDTLLIKSTY-NDDCTDSQLLSANLEFAEPYGQGLTAAEVEQCICPPG 927
BLAST of SMED30010499 vs. Ensembl Celegans
Match: unc-52 (Basement membrane proteoglycan [Source:UniProtKB/Swiss-Prot;Acc:Q06561]) HSP 1 Score: 162.155 bits (409), Expect = 4.200e-39 Identity = 184/706 (26.06%), Postives = 280/706 (39.66%), Query Frame = -2
Query: 6460 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITN--PDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PIN-----------CVECDKNGNCNRCTGQRSGPKCLECKP 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ Y + +G+ ++ ++ ++ + S + ++ + P FL G + +Y ++ I G S P V+L+ N L + V+ S+ + ++I++ E + + +A R D+L VL+D+ LIR + ++QT + + + + PD + A +E+C CP Y G SCE C GYE++ + C PI G C +C GP C C P
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAEGVTISDIEERTIDRNTPFSFFKTGYLTFDGTTDGVAKYWRLPQRFL-------GDKVTAYGGKMEFEIEFSG-SGHHSSEPMVVLKG-NQNILVHRVRNQEHVLRSD-----SPVRITVETYETNY-EQLNGAAATREDLLMVLADLDAFLIRATHVAHQTSTSLGDVSWEIAVDRYTPDGL-----ALEVEQCVCPPGYLGTSCEDCAPGYERSGYGPYLGTCVPIQPRHQQCGPGAVAPTAPAQGQC-QCKASVIGPNCDRCAP 705 HSP 2 Score: 77.0258 bits (188), Expect = 2.577e-13 Identity = 88/342 (25.73%), Postives = 132/342 (38.60%), Query Frame = -2
Query: 6418 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS------------AMG-----------YYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 7356
C F A C+ CFCSG T CS+ A G Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 703 CAPNSFGLAPTNPQGCIPCFCSGVTQQCSASSYRRTSVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDGQ---QATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 1002 HSP 3 Score: 60.8474 bits (146), Expect = 2.260e-8 Identity = 24/61 (39.34%), Postives = 33/61 (54.10%), Query Frame = -3
Query: 6009 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSAD 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR + + + D
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGD 458 HSP 4 Score: 55.0694 bits (131), Expect = 1.157e-6 Identity = 23/60 (38.33%), Postives = 36/60 (60.00%), Query Frame = -3
Query: 6012 GKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSA 6179
G+C+CK + GPNC C+P F + C+ CFCS TQ+C S YR ++++++ A
Sbjct: 687 GQCQCKASVIGPNCDRCAPNSFGLAPTNPQGCIPCFCSGVTQQCSAS-SYRRTSVSIDYA 745
BLAST of SMED30010499 vs. Ensembl Celegans
Match: unc-52 (Basement membrane proteoglycan [Source:UniProtKB/Swiss-Prot;Acc:Q06561]) HSP 1 Score: 162.155 bits (409), Expect = 4.432e-39 Identity = 184/706 (26.06%), Postives = 280/706 (39.66%), Query Frame = -2
Query: 6460 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITN--PDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PIN-----------CVECDKNGNCNRCTGQRSGPKCLECKP 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ Y + +G+ ++ ++ ++ + S + ++ + P FL G + +Y ++ I G S P V+L+ N L + V+ S+ + ++I++ E + + +A R D+L VL+D+ LIR + ++QT + + + + PD + A +E+C CP Y G SCE C GYE++ + C PI G C +C GP C C P
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAEGVTISDIEERTIDRNTPFSFFKTGYLTFDGTTDGVAKYWRLPQRFL-------GDKVTAYGGKMEFEIEFSG-SGHHSSEPMVVLKG-NQNILVHRVRNQEHVLRSD-----SPVRITVETYETNY-EQLNGAAATREDLLMVLADLDAFLIRATHVAHQTSTSLGDVSWEIAVDRYTPDGL-----ALEVEQCVCPPGYLGTSCEDCAPGYERSGYGPYLGTCVPIQPRHQQCGPGAVAPTAPAQGQC-QCKASVIGPNCDRCAP 705 HSP 2 Score: 77.0258 bits (188), Expect = 2.877e-13 Identity = 88/342 (25.73%), Postives = 132/342 (38.60%), Query Frame = -2
Query: 6418 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS------------AMG-----------YYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 7356
C F A C+ CFCSG T CS+ A G Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 703 CAPNSFGLAPTNPQGCIPCFCSGVTQQCSASSYRRTSVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDGQ---QATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 1002 HSP 3 Score: 60.8474 bits (146), Expect = 2.344e-8 Identity = 24/61 (39.34%), Postives = 33/61 (54.10%), Query Frame = -3
Query: 6009 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSAD 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR + + + D
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGD 458 HSP 4 Score: 55.0694 bits (131), Expect = 1.222e-6 Identity = 23/60 (38.33%), Postives = 36/60 (60.00%), Query Frame = -3
Query: 6012 GKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSA 6179
G+C+CK + GPNC C+P F + C+ CFCS TQ+C S YR ++++++ A
Sbjct: 687 GQCQCKASVIGPNCDRCAPNSFGLAPTNPQGCIPCFCSGVTQQCSAS-SYRRTSVSIDYA 745
BLAST of SMED30010499 vs. Ensembl Celegans
Match: unc-52 (Basement membrane proteoglycan [Source:UniProtKB/Swiss-Prot;Acc:Q06561]) HSP 1 Score: 162.155 bits (409), Expect = 4.500e-39 Identity = 184/706 (26.06%), Postives = 280/706 (39.66%), Query Frame = -2
Query: 6460 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITN--PDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PIN-----------CVECDKNGNCNRCTGQRSGPKCLECKP 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ Y + +G+ ++ ++ ++ + S + ++ + P FL G + +Y ++ I G S P V+L+ N L + V+ S+ + ++I++ E + + +A R D+L VL+D+ LIR + ++QT + + + + PD + A +E+C CP Y G SCE C GYE++ + C PI G C +C GP C C P
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAEGVTISDIEERTIDRNTPFSFFKTGYLTFDGTTDGVAKYWRLPQRFL-------GDKVTAYGGKMEFEIEFSG-SGHHSSEPMVVLKG-NQNILVHRVRNQEHVLRSD-----SPVRITVETYETNY-EQLNGAAATREDLLMVLADLDAFLIRATHVAHQTSTSLGDVSWEIAVDRYTPDGL-----ALEVEQCVCPPGYLGTSCEDCAPGYERSGYGPYLGTCVPIQPRHQQCGPGAVAPTAPAQGQC-QCKASVIGPNCDRCAP 705 HSP 2 Score: 77.0258 bits (188), Expect = 2.638e-13 Identity = 88/342 (25.73%), Postives = 132/342 (38.60%), Query Frame = -2
Query: 6418 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS------------AMG-----------YYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 7356
C F A C+ CFCSG T CS+ A G Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 703 CAPNSFGLAPTNPQGCIPCFCSGVTQQCSASSYRRTSVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDGQ---QATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 1002 HSP 3 Score: 60.8474 bits (146), Expect = 2.053e-8 Identity = 24/61 (39.34%), Postives = 33/61 (54.10%), Query Frame = -3
Query: 6009 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSAD 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR + + + D
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGD 458 HSP 4 Score: 55.4546 bits (132), Expect = 1.069e-6 Identity = 23/60 (38.33%), Postives = 36/60 (60.00%), Query Frame = -3
Query: 6012 GKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSA 6179
G+C+CK + GPNC C+P F + C+ CFCS TQ+C S YR ++++++ A
Sbjct: 687 GQCQCKASVIGPNCDRCAPNSFGLAPTNPQGCIPCFCSGVTQQCSAS-SYRRTSVSIDYA 745
BLAST of SMED30010499 vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342810) HSP 1 Score: 183.726 bits (465), Expect = 1.803e-45 Identity = 165/623 (26.48%), Postives = 254/623 (40.77%), Query Frame = -2
Query: 6538 LLVTPTEMIVRPGSSARFLCQLIG-ASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDF---------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA---QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTL----CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSA-MGYYQSPNPKTSTRTKGLLMNFKSQKIL-EVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISL----WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYY-SNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVKKCPINC 8301
L P I++ F C+ G A V W R + R G L I N ++ V E V I + + C+ Q C N CID++ C+G DC D DE+ +C+ +C+PNQF C + KCV WRCDGENDCG+ DE +C + C + F C IP+S CD P+C +G DE C P + P ++ ++ + L C PT TI WR NWG +P + + T L +M P +G Y+CE ++T+ + VT + + CE FN A C++CFC G C SA + Y P S R + ++ Q ++ E + + + + ++ P + L +D+ G+ L+SY L+ + G + + GP VI+ Y + + P G N++ I W+K + A R +I+ +L+++ +LIR+ Y S + N +D T +G+ A +EKC CP Y G SCE C +GY + CV P +C
Sbjct: 1003 LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGRPLPPGFTDRNG----RLEIPNIRVEDAGTYVCEAVGYASYIPGQQVTVNLNVERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQ--SCSLGLKCQPNQFMCS-------NSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSG-HCIPKSFQCDNVPDCTDGTDEVGCMAPLPIRPPPQSVSLLEY-EVLELTCVATGTPTPTIVWRLNWGHVPDKCESKSYGG-------TGTLRCPDMRPQDSGAYSCEIINTRGTHFVNPDTIVTVRPVRTDVCEAGFFNMLARKAEECVQCFCFGVAKACDSANLFTYAIHPPILSHRVVSVELSPLRQIVINEAAPGQDLLTLLHGVQFRATNVHFSGRETPYLALPADYM--GNQLKSYGGNLRYEVNYRGSGRP-VNGPDVIITGNRFTLTYR--------VRTQP-GQNNRVSIPFVPGGWQKPDG-------RKASREEIMMILANVDNILIRLGYLDSTAREVDLINIALDSAGTADKGLGS---ASLVEKCQCPPGYVGDSCESCASGYVRQPGGPWLGHCVPFIPDSC 1581 HSP 2 Score: 80.4925 bits (197), Expect = 3.539e-14 Identity = 78/321 (24.30%), Postives = 133/321 (41.43%), Query Frame = -2
Query: 6451 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKG-----LLMNF--KSQKILEVSYAKSIYDVNSKSYKFVGKMYQD----FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKN--------GNCNRCTGQRSGPKCLECKPGFV 7356
C+ F+ + + C++CFCSG DC S+ +Y+ T R++ L+ N+ + + VS A + F+G Q + P FL + G L Y + + + P V++++ G+ L S+V+ S + ++K S W++ + E A R +L LSDI + I+ Y ++ T+D + P ++G A +E+C CP Y GLSCE+C GY ++ + C EC+ + G+C C+ GP C C G+V
Sbjct: 1687 CKSKSFHLNSFTYTGCIECFCSGVGLDCDSST-WYRDQVTSTFGRSRVDHGFVLVTNYMQPTPDTVPVSMA-----AEPNALSFIGSADQSGNTLYWSLPAAFLGNKLSSYGGKLTYTLSYSPLPNGIMSRNS----APDVVIKS--GEDLRLIHYRKSQVVPSVANTYSVEIKESAWQRGD-------EVVANREHVLMALSDITAIYIKATYTTSTKEASLRQVTLD--VATPTNLGTP-RAVEVEQCRCPEGYLGLSCEQCAPGYARDPEGGIYLGLCRPC-ECNGHSKYCNSDTGDCEECSDNTEGPSCERCAAGYV 1984 HSP 3 Score: 68.5514 bits (166), Expect = 1.372e-10 Identity = 77/307 (25.08%), Postives = 116/307 (37.79%), Query Frame = -2
Query: 6469 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWL--ESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK--ISLWEKENWF-IDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPI---NCVECD--KNGNCN-RCTGQRSGPKCLE 7356
C + +A C +C+CSG C SA Y Q + ++F L + ++ D + MY +L + + GS L + +SY +++ L ES + I+ NG L W S P+G NQ + + L E E W D A R D + VLSD++++LIR N ++ +T +A IE C CP+ Y G SCE C + ++ C CP N CD G RC + G +C E
Sbjct: 2047 CRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYRQL-----------IPVDFILNAPLITDESGAVQDTENLIPDISRNMY---TYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIW----------SRPDGNENQEEYNVRLHEDEQWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTT--RTPGATHASDIELCQCPSGYVGTSCESCAPLHYRDASGSC-SLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCRE 2326 HSP 4 Score: 65.855 bits (159), Expect = 9.746e-10 Identity = 46/141 (32.62%), Postives = 69/141 (48.94%), Query Frame = -2
Query: 7624 DFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYV 8046
D C ++ C +S C+ ++ CNGI +C+DG DE C C + + C+T +CVA+ RC+G DC +G DE +C ++ C + AC N T + R CD +CL+G DE C P Q +
Sbjct: 581 DHCLESEFEC-DSYCLPRDQLCNGIPNCQDGSDER--NCTFCREDAYLCNT-------GECVADNQRCNGIADCADGSDERHCARI---YCPPNKLAC--NGTCVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVI 706 HSP 5 Score: 62.003 bits (149), Expect = 1.234e-8 Identity = 45/143 (31.47%), Postives = 63/143 (44.06%), Query Frame = -2
Query: 7675 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC----------------IKLDKN----TCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK 8043
FC + C C+ +N+ CNGI DC DG DE C PN+ +C+ CV+ +CDG DC +GYDE+ C KL N +C + C N+ I S C+E +C +G DE+
Sbjct: 618 FCREDAYLCNTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACNGT--------CVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVIRPKLGPNPIPKSCRPHEWQC-ANLECIDSSLQCNEIKDCSDGSDEE 751 HSP 6 Score: 62.003 bits (149), Expect = 1.522e-8 Identity = 42/139 (30.22%), Postives = 63/139 (45.32%), Query Frame = -2
Query: 7669 LIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC---IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGE-DEKNC 8073
+ + T + C N+ C N CID K CN + DC +G DE A C ++ C +C++ +CDG +DC +G DE +C + + TC F C D ++ I CD +C N DE+ C
Sbjct: 25 VARGTYLSYGECQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRD------GTRCISVSQQCDGHSDCSDGDDEEHCDGIVPKLRYTCPKGKFTCRD-LSCISIVHRCDGRADCPNDRSDEEGC 156 HSP 7 Score: 60.8474 bits (146), Expect = 3.182e-8 Identity = 53/172 (30.81%), Postives = 68/172 (39.53%), Query Frame = -2
Query: 7639 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC----------AQCEPNQFSCHT---------------------VGGK---PPHQ----KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8040
C ++ C N CID + CN IKDC DG DEE++ C + C P QF C VG P HQ +C RCD C +G DE NC C + F C +N + SA C+ C + DE NC + +PNQ
Sbjct: 719 CRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGRCYTESERCDRHRHCEDGSDEANC-------CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQ 882 HSP 8 Score: 55.8398 bits (133), Expect = 9.156e-7 Identity = 129/574 (22.47%), Postives = 194/574 (33.80%), Query Frame = -3
Query: 4785 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKI-SFTFEYNTDLLKDLVFSDVTPF-----IEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKW-RIGKEGKLLEDRETFIRALMTAQHLSIG----FPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLP---------DIVCG-----RC-------------------DSPDCADCKEG----TYKYDFVLNKNFS---TCQIG-----IEMMTKGR------------------------IIYVNKDESIRIPCTAKSHRGGIAIHKW-------NKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVN--SVSNASEEFFIIVEEKPPRLAVEI-----SVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSS 6212
C G + +C CK N G C +C PG + Q C +C+CS +C+ + YR I +D +L+ I T+ V TEN+I P+ ++ + T + KY L G + + F Y L L+ + I + +W+ NE + ED +W R +E R F+ L QH+ I P + + +V L I E+C CP G G CE+C + Y + P D+V G RC D P D G +++D N+ S T QI I MTK R I+ + K E +P +W N E++ L E E + NL L +Q+SD G+Y+C+AVN + S + I + +K EI VT + V ++ V P +V W V DG DA+ T +
Sbjct: 2020 CQIEGIYDCRGNECLCKRNVIGDQCDQCRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYR--------QLIPVDFILNAPLI--------TDESGAVQDTENLI-----PD------ISRNMYTYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIWSRPDGNENQEEYNVRLHEDE--QWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTTRTPGATHASDI---ELCQCPSGYVGTSCESCAPLHYRDASGSCSLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCREIDTSPIIEDPPQICDLSRGFCCSGFQFDIAPNETISFNDTLQIYKGNRIIGNMTKLRYGCPSRDTNDPTDIGTEDPVLTQIIVSIQKPEITIVPVGGSMTLSCSGRMRWSNSPVIVNWYKENSRLP---ENVEVQGGNLYL--YDLQVSDSGVYICQAVNNETASVFKDTVSITITKKDQLSPAEIVNLPSHVTFEEYVNNEIICEVLGNPAP---RVTWARV-DGHADAQSTRT 2552 HSP 9 Score: 53.1434 bits (126), Expect = 7.195e-6 Identity = 60/253 (23.72%), Postives = 93/253 (36.76%), Query Frame = -2
Query: 7411 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNT--------------------------CLGSHFACDDNVTLIPRSAWCDENPNC-LNGEDEKNC--------------RHP-EVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALS 8040
C NQ C N C+ + CNG +C D DE +C PNQF C++ +CV++ RC+G DC + DE NC C + F C +N I C+ +C +G DE +C R P ++ + + ++ + + +C + + W GR P FT + G+ L I N+ + AG Y CEA+
Sbjct: 838 CYANQFRCNNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCNS-------GQCVSSSVRCNGRTDCQDSSDEQNCGHRHTEVSQGLETTGVFTTSTTSTTAMTPLRIICPPTSFKC-ENGPCISLGLKCNGRVDCPYDGSDEADCGQISNDIDPADSNDRRPNQLNLKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGR-PLPPGFT-DRNGR--------LEIPNIRVEDAGTYVCEAVG 1072
BLAST of SMED30010499 vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342808) HSP 1 Score: 183.341 bits (464), Expect = 2.556e-45 Identity = 165/623 (26.48%), Postives = 254/623 (40.77%), Query Frame = -2
Query: 6538 LLVTPTEMIVRPGSSARFLCQLIG-ASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDF---------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA---QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTL----CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSA-MGYYQSPNPKTSTRTKGLLMNFKSQKIL-EVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISL----WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYY-SNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVKKCPINC 8301
L P I++ F C+ G A V W R + R G L I N ++ V E V I + + C+ Q C N CID++ C+G DC D DE+ +C+ +C+PNQF C + KCV WRCDGENDCG+ DE +C + C + F C IP+S CD P+C +G DE C P + P ++ ++ + L C PT TI WR NWG +P + + T L +M P +G Y+CE ++T+ + VT + + CE FN A C++CFC G C SA + Y P S R + ++ Q ++ E + + + + ++ P + L +D+ G+ L+SY L+ + G + + GP VI+ Y + + P G N++ I W+K + A R +I+ +L+++ +LIR+ Y S + N +D T +G+ A +EKC CP Y G SCE C +GY + CV P +C
Sbjct: 1300 LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGRPLPPGFTDRNG----RLEIPNIRVEDAGTYVCEAVGYASYIPGQQVTVNLNVERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQ--SCSLGLKCQPNQFMCS-------NSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSG-HCIPKSFQCDNVPDCTDGTDEVGCMAPLPIRPPPQSVSLLEY-EVLELTCVATGTPTPTIVWRLNWGHVPDKCESKSYGG-------TGTLRCPDMRPQDSGAYSCEIINTRGTHFVNPDTIVTVRPVRTDVCEAGFFNMLARKAEECVQCFCFGVAKACDSANLFTYAIHPPILSHRVVSVELSPLRQIVINEAAPGQDLLTLLHGVQFRATNVHFSGRETPYLALPADYM--GNQLKSYGGNLRYEVNYRGSGRP-VNGPDVIITGNRFTLTYR--------VRTQP-GQNNRVSIPFVPGGWQKPDG-------RKASREEIMMILANVDNILIRLGYLDSTAREVDLINIALDSAGTADKGLGS---ASLVEKCQCPPGYVGDSCESCASGYVRQPGGPWLGHCVPFIPDSC 1878 HSP 2 Score: 80.4925 bits (197), Expect = 3.647e-14 Identity = 78/321 (24.30%), Postives = 133/321 (41.43%), Query Frame = -2
Query: 6451 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKG-----LLMNF--KSQKILEVSYAKSIYDVNSKSYKFVGKMYQD----FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKN--------GNCNRCTGQRSGPKCLECKPGFV 7356
C+ F+ + + C++CFCSG DC S+ +Y+ T R++ L+ N+ + + VS A + F+G Q + P FL + G L Y + + + P V++++ G+ L S+V+ S + ++K S W++ + E A R +L LSDI + I+ Y ++ T+D + P ++G A +E+C CP Y GLSCE+C GY ++ + C EC+ + G+C C+ GP C C G+V
Sbjct: 1984 CKSKSFHLNSFTYTGCIECFCSGVGLDCDSST-WYRDQVTSTFGRSRVDHGFVLVTNYMQPTPDTVPVSMA-----AEPNALSFIGSADQSGNTLYWSLPAAFLGNKLSSYGGKLTYTLSYSPLPNGIMSRNS----APDVVIKS--GEDLRLIHYRKSQVVPSVANTYSVEIKESAWQRGD-------EVVANREHVLMALSDITAIYIKATYTTSTKEASLRQVTLD--VATPTNLGT-PRAVEVEQCRCPEGYLGLSCEQCAPGYARDPEGGIYLGLCRPC-ECNGHSKYCNSDTGDCEECSDNTEGPSCERCAAGYV 2281 HSP 3 Score: 68.5514 bits (166), Expect = 1.412e-10 Identity = 77/307 (25.08%), Postives = 116/307 (37.79%), Query Frame = -2
Query: 6469 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWL--ESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK--ISLWEKENWF-IDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPI---NCVECD--KNGNCN-RCTGQRSGPKCLE 7356
C + +A C +C+CSG C SA Y Q + ++F L + ++ D + MY +L + + GS L + +SY +++ L ES + I+ NG L W S P+G NQ + + L E E W D A R D + VLSD++++LIR N ++ +T +A IE C CP+ Y G SCE C + ++ C CP N CD G RC + G +C E
Sbjct: 2344 CRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYRQL-----------IPVDFILNAPLITDESGAVQDTENLIPDISRNMY---TYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIW----------SRPDGNENQEEYNVRLHEDEQWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTT--RTPGATHASDIELCQCPSGYVGTSCESCAPLHYRDASGSC-SLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCRE 2623 HSP 4 Score: 67.781 bits (164), Expect = 2.334e-10 Identity = 121/511 (23.68%), Postives = 191/511 (37.38%), Query Frame = -3
Query: 4791 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIIL-VKRNP---NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKW-RIGKEGKLLEDRETFIRALMTAQHLSIG----FPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLP---------DIVCG-----RCDSPDCAD-CKE-GTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSH-RGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVN--SVSNASEEFFIIVEEKPPRLAVEI-----SVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKET 6212
C G + +C CK N G C +C PG + Q C +C+CS +C+ + YR I +D +L+ I T+ V TEN+I + RN + L S + + + YGG++S++ +++ + I + +W+ NE + ED +W R +E R F+ L QH+ I P + + +V L I E+C CP G G CE+C + Y + P D+V G RC + D C+E T + ++ QI I + K I V S+ + C+ + I W K E++ L E E + NL L +Q+SD G+Y+C+AVN + S + I + +K EI VT + V ++ V P +V W V DG DA+ T
Sbjct: 2317 CQIEGIYDCRGNECLCKRNVIGDQCDQCRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYR--------QLIPVDFILNAPLI--------TDESGAVQDTENLIPDISRNMYTYTHTSYLPKYWSLRGSVLGNQL----FSYGGRLSYSL-----IVESYGNYERGHDIVLIGNGLKLIWSRPDGNENQEEYNVRLHEDE--QWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTTRTPGATHASDI---ELCQCPSGYVGTSCESCAPLHYRDASGSCSLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCREIDTNDPTDIGTEDPVLTQI-IVSIQKPEITIVPVGGSMTLSCSGRMRWSNSPVIVNWYK--ENSRL---PENVEVQGGNLYL--YDLQVSDSGVYICQAVNNETASVFKDTVSITITKKDQLSPAEIVNLPSHVTFEEYVNNEIICEVLGNPAP---RVTWARV-DGHADAQST 2785 HSP 5 Score: 65.855 bits (159), Expect = 8.847e-10 Identity = 46/141 (32.62%), Postives = 69/141 (48.94%), Query Frame = -2
Query: 7624 DFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYV 8046
D C ++ C +S C+ ++ CNGI +C+DG DE C C + + C+T +CVA+ RC+G DC +G DE +C ++ C + AC N T + R CD +CL+G DE C P Q +
Sbjct: 955 DHCLESEFEC-DSYCLPRDQLCNGIPNCQDGSDER--NCTFCREDAYLCNT-------GECVADNQRCNGIADCADGSDERHCARI---YCPPNKLAC--NGTCVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVI 1080 HSP 6 Score: 62.3882 bits (150), Expect = 1.120e-8 Identity = 45/143 (31.47%), Postives = 63/143 (44.06%), Query Frame = -2
Query: 7675 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC----------------IKLDKN----TCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK 8043
FC + C C+ +N+ CNGI DC DG DE C PN+ +C+ CV+ +CDG DC +GYDE+ C KL N +C + C N+ I S C+E +C +G DE+
Sbjct: 992 FCREDAYLCNTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACNGT--------CVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVIRPKLGPNPIPKSCRPHEWQC-ANLECIDSSLQCNEIKDCSDGSDEE 1125 HSP 7 Score: 61.2326 bits (147), Expect = 2.361e-8 Identity = 42/130 (32.31%), Postives = 56/130 (43.08%), Query Frame = -2
Query: 7657 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC--IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPE 8040
C N+ C N CID K CN + DC +G DE A C ++ C +C++ +CDG +DC +G DE +C D C F C IP CD +C + DE NC E
Sbjct: 492 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRD------GTRCISVSQQCDGHSDCSDGDDEEHCDGSGYDSEECRFDEFHCGTG-ECIPMRQVCDNIYDCNDYSDEVNCVEGE 614 HSP 8 Score: 60.8474 bits (146), Expect = 2.655e-8 Identity = 53/172 (30.81%), Postives = 68/172 (39.53%), Query Frame = -2
Query: 7639 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC----------AQCEPNQFSCHT---------------------VGGK---PPHQ----KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8040
C ++ C N CID + CN IKDC DG DEE++ C + C P QF C VG P HQ +C RCD C +G DE NC C + F C +N + SA C+ C + DE NC + +PNQ
Sbjct: 1093 CRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGRCYTESERCDRHRHCEDGSDEANC-------CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQ 1256 HSP 9 Score: 56.6102 bits (135), Expect = 6.408e-7 Identity = 55/211 (26.07%), Postives = 80/211 (37.91%), Query Frame = -2
Query: 7411 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALS 8040
C NQ C N C+ + CNG +C D DE +C PNQF C++ +CV++ RC+G DC + DE NC A D N D PN LN + + ++ + + +C + + W GR P FT + G+ L I N+ + AG Y CEA+
Sbjct: 1212 CYANQFRCNNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCNS-------GQCVSSSVRCNGRTDCQDSSDEQNC-------------AADSN----------DRRPNQLN-------------LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGR-PLPPGFT-DRNGR--------LEIPNIRVEDAGTYVCEAVG 1369
BLAST of SMED30010499 vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342816) HSP 1 Score: 183.341 bits (464), Expect = 2.739e-45 Identity = 165/623 (26.48%), Postives = 254/623 (40.77%), Query Frame = -2
Query: 6538 LLVTPTEMIVRPGSSARFLCQLIG-ASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDF---------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA---QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTL----CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSA-MGYYQSPNPKTSTRTKGLLMNFKSQKIL-EVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISL----WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYY-SNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVKKCPINC 8301
L P I++ F C+ G A V W R + R G L I N ++ V E V I + + C+ Q C N CID++ C+G DC D DE+ +C+ +C+PNQF C + KCV WRCDGENDCG+ DE +C + C + F C IP+S CD P+C +G DE C P + P ++ ++ + L C PT TI WR NWG +P + + T L +M P +G Y+CE ++T+ + VT + + CE FN A C++CFC G C SA + Y P S R + ++ Q ++ E + + + + ++ P + L +D+ G+ L+SY L+ + G + + GP VI+ Y + + P G N++ I W+K + A R +I+ +L+++ +LIR+ Y S + N +D T +G+ A +EKC CP Y G SCE C +GY + CV P +C
Sbjct: 1188 LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGRPLPPGFTDRNG----RLEIPNIRVEDAGTYVCEAVGYASYIPGQQVTVNLNVERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQ--SCSLGLKCQPNQFMCS-------NSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSG-HCIPKSFQCDNVPDCTDGTDEVGCMAPLPIRPPPQSVSLLEY-EVLELTCVATGTPTPTIVWRLNWGHVPDKCESKSYGG-------TGTLRCPDMRPQDSGAYSCEIINTRGTHFVNPDTIVTVRPVRTDVCEAGFFNMLARKAEECVQCFCFGVAKACDSANLFTYAIHPPILSHRVVSVELSPLRQIVINEAAPGQDLLTLLHGVQFRATNVHFSGRETPYLALPADYM--GNQLKSYGGNLRYEVNYRGSGRP-VNGPDVIITGNRFTLTYR--------VRTQP-GQNNRVSIPFVPGGWQKPDG-------RKASREEIMMILANVDNILIRLGYLDSTAREVDLINIALDSAGTADKGLGS---ASLVEKCQCPPGYVGDSCESCASGYVRQPGGPWLGHCVPFIPDSC 1766 HSP 2 Score: 80.4925 bits (197), Expect = 3.375e-14 Identity = 78/321 (24.30%), Postives = 133/321 (41.43%), Query Frame = -2
Query: 6451 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKG-----LLMNF--KSQKILEVSYAKSIYDVNSKSYKFVGKMYQD----FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKN--------GNCNRCTGQRSGPKCLECKPGFV 7356
C+ F+ + + C++CFCSG DC S+ +Y+ T R++ L+ N+ + + VS A + F+G Q + P FL + G L Y + + + P V++++ G+ L S+V+ S + ++K S W++ + E A R +L LSDI + I+ Y ++ T+D + P ++G A +E+C CP Y GLSCE+C GY ++ + C EC+ + G+C C+ GP C C G+V
Sbjct: 1872 CKSKSFHLNSFTYTGCIECFCSGVGLDCDSST-WYRDQVTSTFGRSRVDHGFVLVTNYMQPTPDTVPVSMA-----AEPNALSFIGSADQSGNTLYWSLPAAFLGNKLSSYGGKLTYTLSYSPLPNGIMSRNS----APDVVIKS--GEDLRLIHYRKSQVVPSVANTYSVEIKESAWQRGD-------EVVANREHVLMALSDITAIYIKATYTTSTKEASLRQVTLD--VATPTNLGT-PRAVEVEQCRCPEGYLGLSCEQCAPGYARDPEGGIYLGLCRPC-ECNGHSKYCNSDTGDCEECSDNTEGPSCERCAAGYV 2169 HSP 3 Score: 68.5514 bits (166), Expect = 1.352e-10 Identity = 77/307 (25.08%), Postives = 116/307 (37.79%), Query Frame = -2
Query: 6469 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWL--ESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK--ISLWEKENWF-IDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP---INCVECD--KNGNCN-RCTGQRSGPKCLE 7356
C + +A C +C+CSG C SA Y Q + ++F L + ++ D + MY +L + + GS L + +SY +++ L ES + I+ NG L W S P+G NQ + + L E E W D A R D + VLSD++++LIR N ++ +T +A IE C CP+ Y G SCE C + ++ C CP N CD G RC + G +C E
Sbjct: 2232 CRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYRQL-----------IPVDFILNAPLITDESGAVQDTENLIPDISRNMY---TYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIW----------SRPDGNENQEEYNVRLHEDEQWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTT--RTPGATHASDIELCQCPSGYVGTSCESCAPLHYRDASGSC-SLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCRE 2511 HSP 4 Score: 68.1662 bits (165), Expect = 1.694e-10 Identity = 121/511 (23.68%), Postives = 191/511 (37.38%), Query Frame = -3
Query: 4791 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIIL-VKRNP---NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKW-RIGKEGKLLEDRETFIRALMTAQHLSIG----FPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLP---------DIVCG-----RCDSPDCAD-CKE-GTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSH-RGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVN--SVSNASEEFFIIVEEKPPRLAVEI-----SVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKET 6212
C G + +C CK N G C +C PG + Q C +C+CS +C+ + YR I +D +L+ I T+ V TEN+I + RN + L S + + + YGG++S++ +++ + I + +W+ NE + ED +W R +E R F+ L QH+ I P + + +V L I E+C CP G G CE+C + Y + P D+V G RC + D C+E T + ++ QI I + K I V S+ + C+ + I W K E++ L E E + NL L +Q+SD G+Y+C+AVN + S + I + +K EI VT + V ++ V P +V W V DG DA+ T
Sbjct: 2205 CQIEGIYDCRGNECLCKRNVIGDQCDQCRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYR--------QLIPVDFILNAPLI--------TDESGAVQDTENLIPDISRNMYTYTHTSYLPKYWSLRGSVLGNQL----FSYGGRLSYSL-----IVESYGNYERGHDIVLIGNGLKLIWSRPDGNENQEEYNVRLHEDE--QWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTTRTPGATHASDI---ELCQCPSGYVGTSCESCAPLHYRDASGSCSLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCREIDTNDPTDIGTEDPVLTQI-IVSIQKPEITIVPVGGSMTLSCSGRMRWSNSPVIVNWYK--ENSRL---PENVEVQGGNLYL--YDLQVSDSGVYICQAVNNETASVFKDTVSITITKKDQLSPAEIVNLPSHVTFEEYVNNEIICEVLGNPAP---RVTWARV-DGHADAQST 2673 HSP 5 Score: 61.2326 bits (147), Expect = 2.205e-8 Identity = 42/130 (32.31%), Postives = 56/130 (43.08%), Query Frame = -2
Query: 7657 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC--IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPE 8040
C N+ C N CID K CN + DC +G DE A C ++ C +C++ +CDG +DC +G DE +C D C F C IP CD +C + DE NC E
Sbjct: 492 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRD------GTRCISVSQQCDGHSDCSDGDDEEHCDGSGYDSEECRFDEFHCGTG-ECIPMRQVCDNIYDCNDYSDEVNCVEGE 614 HSP 6 Score: 61.2326 bits (147), Expect = 2.585e-8 Identity = 53/172 (30.81%), Postives = 68/172 (39.53%), Query Frame = -2
Query: 7639 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC----------AQCEPNQFSCHT---------------------VGGK---PPHQ----KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8040
C ++ C N CID + CN IKDC DG DEE++ C + C P QF C VG P HQ +C RCD C +G DE NC C + F C +N + SA C+ C + DE NC + +PNQ
Sbjct: 981 CRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGRCYTESERCDRHRHCEDGSDEANC-------CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQ 1144 HSP 7 Score: 56.6102 bits (135), Expect = 5.691e-7 Identity = 55/211 (26.07%), Postives = 80/211 (37.91%), Query Frame = -2
Query: 7411 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALS 8040
C NQ C N C+ + CNG +C D DE +C PNQF C++ +CV++ RC+G DC + DE NC A D N D PN LN + + ++ + + +C + + W GR P FT + G+ L I N+ + AG Y CEA+
Sbjct: 1100 CYANQFRCNNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCNS-------GQCVSSSVRCNGRTDCQDSSDEQNC-------------AADSN----------DRRPNQLN-------------LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGR-PLPPGFT-DRNGR--------LEIPNIRVEDAGTYVCEAVG 1257
BLAST of SMED30010499 vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342814) HSP 1 Score: 183.341 bits (464), Expect = 2.877e-45 Identity = 165/623 (26.48%), Postives = 254/623 (40.77%), Query Frame = -2
Query: 6538 LLVTPTEMIVRPGSSARFLCQLIG-ASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDF---------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA---QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTL----CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSA-MGYYQSPNPKTSTRTKGLLMNFKSQKIL-EVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISL----WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYY-SNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVKKCPINC 8301
L P I++ F C+ G A V W R + R G L I N ++ V E V I + + C+ Q C N CID++ C+G DC D DE+ +C+ +C+PNQF C + KCV WRCDGENDCG+ DE +C + C + F C IP+S CD P+C +G DE C P + P ++ ++ + L C PT TI WR NWG +P + + T L +M P +G Y+CE ++T+ + VT + + CE FN A C++CFC G C SA + Y P S R + ++ Q ++ E + + + + ++ P + L +D+ G+ L+SY L+ + G + + GP VI+ Y + + P G N++ I W+K + A R +I+ +L+++ +LIR+ Y S + N +D T +G+ A +EKC CP Y G SCE C +GY + CV P +C
Sbjct: 776 LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGRPLPPGFTDRNG----RLEIPNIRVEDAGTYVCEAVGYASYIPGQQVTVNLNVERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQ--SCSLGLKCQPNQFMCS-------NSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSG-HCIPKSFQCDNVPDCTDGTDEVGCMAPLPIRPPPQSVSLLEY-EVLELTCVATGTPTPTIVWRLNWGHVPDKCESKSYGG-------TGTLRCPDMRPQDSGAYSCEIINTRGTHFVNPDTIVTVRPVRTDVCEAGFFNMLARKAEECVQCFCFGVAKACDSANLFTYAIHPPILSHRVVSVELSPLRQIVINEAAPGQDLLTLLHGVQFRATNVHFSGRETPYLALPADYM--GNQLKSYGGNLRYEVNYRGSGRP-VNGPDVIITGNRFTLTYR--------VRTQP-GQNNRVSIPFVPGGWQKPDG-------RKASREEIMMILANVDNILIRLGYLDSTAREVDLINIALDSAGTADKGLGS---ASLVEKCQCPPGYVGDSCESCASGYVRQPGGPWLGHCVPFIPDSC 1354 HSP 2 Score: 80.4925 bits (197), Expect = 3.233e-14 Identity = 78/321 (24.30%), Postives = 133/321 (41.43%), Query Frame = -2
Query: 6451 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKG-----LLMNF--KSQKILEVSYAKSIYDVNSKSYKFVGKMYQD----FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKN--------GNCNRCTGQRSGPKCLECKPGFV 7356
C+ F+ + + C++CFCSG DC S+ +Y+ T R++ L+ N+ + + VS A + F+G Q + P FL + G L Y + + + P V++++ G+ L S+V+ S + ++K S W++ + E A R +L LSDI + I+ Y ++ T+D + P ++G A +E+C CP Y GLSCE+C GY ++ + C EC+ + G+C C+ GP C C G+V
Sbjct: 1460 CKSKSFHLNSFTYTGCIECFCSGVGLDCDSST-WYRDQVTSTFGRSRVDHGFVLVTNYMQPTPDTVPVSMA-----AEPNALSFIGSADQSGNTLYWSLPAAFLGNKLSSYGGKLTYTLSYSPLPNGIMSRNS----APDVVIKS--GEDLRLIHYRKSQVVPSVANTYSVEIKESAWQRGD-------EVVANREHVLMALSDITAIYIKATYTTSTKEASLRQVTLD--VATPTNLGTP-RAVEVEQCRCPEGYLGLSCEQCAPGYARDPEGGIYLGLCRPC-ECNGHSKYCNSDTGDCEECSDNTEGPSCERCAAGYV 1757 HSP 3 Score: 68.9366 bits (167), Expect = 1.125e-10 Identity = 121/511 (23.68%), Postives = 191/511 (37.38%), Query Frame = -3
Query: 4791 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIIL-VKRNP---NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKW-RIGKEGKLLEDRETFIRALMTAQHLSIG----FPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLP---------DIVCG-----RCDSPDCAD-CKE-GTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSH-RGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVN--SVSNASEEFFIIVEEKPPRLAVEI-----SVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKET 6212
C G + +C CK N G C +C PG + Q C +C+CS +C+ + YR I +D +L+ I T+ V TEN+I + RN + L S + + + YGG++S++ +++ + I + +W+ NE + ED +W R +E R F+ L QH+ I P + + +V L I E+C CP G G CE+C + Y + P D+V G RC + D C+E T + ++ QI I + K I V S+ + C+ + I W K E++ L E E + NL L +Q+SD G+Y+C+AVN + S + I + +K EI VT + V ++ V P +V W V DG DA+ T
Sbjct: 1793 CQIEGIYDCRGNECLCKRNVIGDQCDQCRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYR--------QLIPVDFILNAPLI--------TDESGAVQDTENLIPDISRNMYTYTHTSYLPKYWSLRGSVLGNQL----FSYGGRLSYSL-----IVESYGNYERGHDIVLIGNGLKLIWSRPDGNENQEEYNVRLHEDE--QWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTTRTPGATHASDI---ELCQCPSGYVGTSCESCAPLHYRDASGSCSLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCREIDTNDPTDIGTEDPVLTQI-IVSIQKPEITIVPVGGSMTLSCSGRMRWSNSPVIVNWYK--ENSRL---PENVEVQGGNLYL--YDLQVSDSGVYICQAVNNETASVFKDTVSITITKKDQLSPAEIVNLPSHVTFEEYVNNEIICEVLGNPAP---RVTWARV-DGHADAQST 2261 HSP 4 Score: 68.5514 bits (166), Expect = 1.459e-10 Identity = 77/307 (25.08%), Postives = 116/307 (37.79%), Query Frame = -2
Query: 6469 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWL--ESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK--ISLWEKENWF-IDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP---INCVECD--KNGNCN-RCTGQRSGPKCLE 7356
C + +A C +C+CSG C SA Y Q + ++F L + ++ D + MY +L + + GS L + +SY +++ L ES + I+ NG L W S P+G NQ + + L E E W D A R D + VLSD++++LIR N ++ +T +A IE C CP+ Y G SCE C + ++ C CP N CD G RC + G +C E
Sbjct: 1820 CRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYRQL-----------IPVDFILNAPLITDESGAVQDTENLIPDISRNMY---TYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIW----------SRPDGNENQEEYNVRLHEDEQWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTT--RTPGATHASDIELCQCPSGYVGTSCESCAPLHYRDASGSC-SLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCRE 2099 HSP 5 Score: 62.7734 bits (151), Expect = 7.619e-9 Identity = 43/128 (33.59%), Postives = 59/128 (46.09%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFD--EEINTCAQC--EPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C N+ C N CID K CN + DC +G D EE ++ Q E +F+CH ++ C++ CDG DCG DE + + C G + C IP+S CD P C + DE C
Sbjct: 492 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECHSPPQTCLENIEFACH-------NRDCISIESVCDGIPDCGRNEDEDDAL----CKCSGDKYKCQRGGGCIPKSQVCDGKPQCHDRSDESAC 608 HSP 6 Score: 56.9954 bits (136), Expect = 3.815e-7 Identity = 55/211 (26.07%), Postives = 80/211 (37.91%), Query Frame = -2
Query: 7411 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALS 8040
C NQ C N C+ + CNG +C D DE +C PNQF C++ +CV++ RC+G DC + DE NC A D N D PN LN + + ++ + + +C + + W GR P FT + G+ L I N+ + AG Y CEA+
Sbjct: 688 CYANQFRCNNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCNS-------GQCVSSSVRCNGRTDCQDSSDEQNC-------------AADSN----------DRRPNQLN-------------LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGR-PLPPGFT-DRNGR--------LEIPNIRVEDAGTYVCEAVG 845
BLAST of SMED30010499 vs. Ensembl Fly
Match: trol (gene:FBgn0284408 transcript:FBtr0342819) HSP 1 Score: 183.341 bits (464), Expect = 2.983e-45 Identity = 165/623 (26.48%), Postives = 254/623 (40.77%), Query Frame = -2
Query: 6538 LLVTPTEMIVRPGSSARFLCQLIG-ASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDF---------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA---QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTL----CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSA-MGYYQSPNPKTSTRTKGLLMNFKSQKIL-EVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISL----WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYY-SNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVKKCPINC 8301
L P I++ F C+ G A V W R + R G L I N ++ V E V I + + C+ Q C N CID++ C+G DC D DE+ +C+ +C+PNQF C + KCV WRCDGENDCG+ DE +C + C + F C IP+S CD P+C +G DE C P + P ++ ++ + L C PT TI WR NWG +P + + T L +M P +G Y+CE ++T+ + VT + + CE FN A C++CFC G C SA + Y P S R + ++ Q ++ E + + + + ++ P + L +D+ G+ L+SY L+ + G + + GP VI+ Y + + P G N++ I W+K + A R +I+ +L+++ +LIR+ Y S + N +D T +G+ A +EKC CP Y G SCE C +GY + CV P +C
Sbjct: 1340 LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGRPLPPGFTDRNG----RLEIPNIRVEDAGTYVCEAVGYASYIPGQQVTVNLNVERYNDVGSRPESACTEYQATCMNGECIDKSSICDGNPDCSDASDEQ--SCSLGLKCQPNQFMCS-------NSKCVDRTWRCDGENDCGDNSDETSCDPEPSGAPCRYNEFQCRSG-HCIPKSFQCDNVPDCTDGTDEVGCMAPLPIRPPPQSVSLLEY-EVLELTCVATGTPTPTIVWRLNWGHVPDKCESKSYGG-------TGTLRCPDMRPQDSGAYSCEIINTRGTHFVNPDTIVTVRPVRTDVCEAGFFNMLARKAEECVQCFCFGVAKACDSANLFTYAIHPPILSHRVVSVELSPLRQIVINEAAPGQDLLTLLHGVQFRATNVHFSGRETPYLALPADYM--GNQLKSYGGNLRYEVNYRGSGRP-VNGPDVIITGNRFTLTYR--------VRTQP-GQNNRVSIPFVPGGWQKPDG-------RKASREEIMMILANVDNILIRLGYLDSTAREVDLINIALDSAGTADKGLGS---ASLVEKCQCPPGYVGDSCESCASGYVRQPGGPWLGHCVPFIPDSC 1918 HSP 2 Score: 80.4925 bits (197), Expect = 3.776e-14 Identity = 78/321 (24.30%), Postives = 133/321 (41.43%), Query Frame = -2
Query: 6451 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKG-----LLMNF--KSQKILEVSYAKSIYDVNSKSYKFVGKMYQD----FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKN--------GNCNRCTGQRSGPKCLECKPGFV 7356
C+ F+ + + C++CFCSG DC S+ +Y+ T R++ L+ N+ + + VS A + F+G Q + P FL + G L Y + + + P V++++ G+ L S+V+ S + ++K S W++ + E A R +L LSDI + I+ Y ++ T+D + P ++G A +E+C CP Y GLSCE+C GY ++ + C EC+ + G+C C+ GP C C G+V
Sbjct: 2024 CKSKSFHLNSFTYTGCIECFCSGVGLDCDSST-WYRDQVTSTFGRSRVDHGFVLVTNYMQPTPDTVPVSMA-----AEPNALSFIGSADQSGNTLYWSLPAAFLGNKLSSYGGKLTYTLSYSPLPNGIMSRNS----APDVVIKS--GEDLRLIHYRKSQVVPSVANTYSVEIKESAWQRGD-------EVVANREHVLMALSDITAIYIKATYTTSTKEASLRQVTLD--VATPTNLGT-PRAVEVEQCRCPEGYLGLSCEQCAPGYARDPEGGIYLGLCRPC-ECNGHSKYCNSDTGDCEECSDNTEGPSCERCAAGYV 2321 HSP 3 Score: 68.5514 bits (166), Expect = 1.356e-10 Identity = 77/307 (25.08%), Postives = 116/307 (37.79%), Query Frame = -2
Query: 6469 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWL--ESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK--ISLWEKENWF-IDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPI---NCVECD--KNGNCN-RCTGQRSGPKCLE 7356
C + +A C +C+CSG C SA Y Q + ++F L + ++ D + MY +L + + GS L + +SY +++ L ES + I+ NG L W S P+G NQ + + L E E W D A R D + VLSD++++LIR N ++ +T +A IE C CP+ Y G SCE C + ++ C CP N CD G RC + G +C E
Sbjct: 2384 CRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYRQL-----------IPVDFILNAPLITDESGAVQDTENLIPDISRNMY---TYTHTSYLPKYWSLRGSVLGNQLFSYGGRLSYSLIVESYGNYERGHDIVLIGNGLKLIW----------SRPDGNENQEEYNVRLHEDEQWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTT--RTPGATHASDIELCQCPSGYVGTSCESCAPLHYRDASGSC-SLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCRE 2663 HSP 4 Score: 67.781 bits (164), Expect = 2.131e-10 Identity = 121/513 (23.59%), Postives = 192/513 (37.43%), Query Frame = -3
Query: 4785 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIIL-VKRNP---NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKW-RIGKEGKLLEDRETFIRALMTAQHLSIG----FPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLP---------DIVCG-----RCDSPDCAD-CKE-GTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSH-RGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVN--SVSNASEEFFIIVEEKPPRLAVEI-----SVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSS 6212
C G + +C CK N G C +C PG + Q C +C+CS +C+ + YR I +D +L+ I T+ V TEN+I + RN + L S + + + YGG++S++ +++ + I + +W+ NE + ED +W R +E R F+ L QH+ I P + + +V L I E+C CP G G CE+C + Y + P D+V G RC + D C+E T + ++ QI I + K I V S+ + C+ + I W K E++ L E E + NL L +Q+SD G+Y+C+AVN + S + I + +K EI VT + V ++ V P +V W V DG DA+ T +
Sbjct: 2357 CQIEGIYDCRGNECLCKRNVIGDQCDQCRPGTYGLSAQNQDGCKECYCSGLASQCRSAALYR--------QLIPVDFILNAPLI--------TDESGAVQDTENLIPDISRNMYTYTHTSYLPKYWSLRGSVLGNQL----FSYGGRLSYSL-----IVESYGNYERGHDIVLIGNGLKLIWSRPDGNENQEEYNVRLHEDE--QWTRQDRESARPASRSDFMTVLSDLQHILIRATPRVPTQSTSIGNVILESAVTTRTPGATHASDI---ELCQCPSGYVGTSCESCAPLHYRDASGSCSLCPCDVSNTESCDLVSGGYVECRCKARWKGDRCREIDTNDPTDIGTEDPVLTQI-IVSIQKPEITIVPVGGSMTLSCSGRMRWSNSPVIVNWYK--ENSRL---PENVEVQGGNLYL--YDLQVSDSGVYICQAVNNETASVFKDTVSITITKKDQLSPAEIVNLPSHVTFEEYVNNEIICEVLGNPAP---RVTWARV-DGHADAQSTRT 2827 HSP 5 Score: 65.855 bits (159), Expect = 8.930e-10 Identity = 46/141 (32.62%), Postives = 69/141 (48.94%), Query Frame = -2
Query: 7624 DFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYV 8046
D C ++ C +S C+ ++ CNGI +C+DG DE C C + + C+T +CVA+ RC+G DC +G DE +C ++ C + AC N T + R CD +CL+G DE C P Q +
Sbjct: 995 DHCLESEFEC-DSYCLPRDQLCNGIPNCQDGSDER--NCTFCREDAYLCNT-------GECVADNQRCNGIADCADGSDERHCARI---YCPPNKLAC--NGTCVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVI 1120 HSP 6 Score: 62.3882 bits (150), Expect = 1.103e-8 Identity = 45/143 (31.47%), Postives = 63/143 (44.06%), Query Frame = -2
Query: 7675 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC----------------IKLDKN----TCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK 8043
FC + C C+ +N+ CNGI DC DG DE C PN+ +C+ CV+ +CDG DC +GYDE+ C KL N +C + C N+ I S C+E +C +G DE+
Sbjct: 1032 FCREDAYLCNTGECVADNQRCNGIADCADGSDERHCARIYCPPNKLACNGT--------CVSRRIKCDGIRDCLDGYDEMYCPETNNHYPTQNVNVIRPKLGPNPIPKSCRPHEWQC-ANLECIDSSLQCNEIKDCSDGSDEE 1165 HSP 7 Score: 61.2326 bits (147), Expect = 2.305e-8 Identity = 42/130 (32.31%), Postives = 56/130 (43.08%), Query Frame = -2
Query: 7657 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC--IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPE 8040
C N+ C N CID K CN + DC +G DE A C ++ C +C++ +CDG +DC +G DE +C D C F C IP CD +C + DE NC E
Sbjct: 532 CQANEFRCNNGDCIDARKRCNNVSDCSEGEDENEECPAACSGMEYQCRD------GTRCISVSQQCDGHSDCSDGDDEEHCDGSGYDSEECRFDEFHCGTG-ECIPMRQVCDNIYDCNDYSDEVNCVEGE 654 HSP 8 Score: 61.2326 bits (147), Expect = 2.591e-8 Identity = 53/172 (30.81%), Postives = 68/172 (39.53%), Query Frame = -2
Query: 7639 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC----------AQCEPNQFSCHT---------------------VGGK---PPHQ----KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8040
C ++ C N CID + CN IKDC DG DEE++ C + C P QF C VG P HQ +C RCD C +G DE NC C + F C +N + SA C+ C + DE NC + +PNQ
Sbjct: 1133 CRPHEWQCANLECIDSSLQCNEIKDCSDGSDEELSVCFGTATTRLKPSDCSPEQFYCDESCYNRSVRCNGHVDCSDGSDEVGCSLPCPQHQCPSGRCYTESERCDRHRHCEDGSDEANC-------CYANQFRC-NNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQ 1296 HSP 9 Score: 56.6102 bits (135), Expect = 6.360e-7 Identity = 55/211 (26.07%), Postives = 80/211 (37.91%), Query Frame = -2
Query: 7411 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALS 8040
C NQ C N C+ + CNG +C D DE +C PNQF C++ +CV++ RC+G DC + DE NC A D N D PN LN + + ++ + + +C + + W GR P FT + G+ L I N+ + AG Y CEA+
Sbjct: 1252 CYANQFRCNNGDCVSGSAPCNGYSECSDHSDELNCGGTQECLPNQFRCNS-------GQCVSSSVRCNGRTDCQDSSDEQNC-------------AADSN----------DRRPNQLN-------------LKTYPDSQIIKESREVIFRCRDEGPARAKVKWSRPGGR-PLPPGFT-DRNGR--------LEIPNIRVEDAGTYVCEAVG 1409
BLAST of SMED30010499 vs. Ensembl Zebrafish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 206.068 bits (523), Expect = 5.945e-62 Identity = 168/616 (27.27%), Postives = 263/616 (42.69%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFT-VHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEK--PMFNNAAMFVS------------MCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCK--CTGRERNDPLCDKC 8040
C +Q C+N CI C+G DC DG DE T + CEPN+F C + +C WRCDG+NDC + DE+NC K C F C + IP S CDE P+C + DE C P V + +E+ + +++ C + VP I WR NWG +P T + + G+ LTI ++ G YTCEA++ K + + I +L +K P+ +++ ++ C+ CFC G T C S Y + + KG+ ++F S+ L + + +N ++ +F +D + F + G+ ++SY YLK + + P P V+L K +Y E + + +N +I E ENW + + + R D++ L++++ + IR Y + + ++D GN PR +E+C CP Y+GLSCE C G+E+ C C + CD +G+C C + G +C +CKPGF DC C C + S+ C D Q C+ TGR C+KC
Sbjct: 264 CRADQATCQNGQCISREYLCDGESDCTDGSDESRCGTPSPCEPNEFKCQ-------NGRCALKLWRCDGDNDCQDNSDEMNCPTKRPGAACAPEQFVCLSDRKCIPASYQCDEEPDCADRSDEFGCAPPTVTVSPEESITASRGD-TVTFTCSAIGVPVPIITWRLNWGHIPANNRITMISENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLV---FAIPDGVLSLSQKPNPVVSSSPFILAGNCRDGHFSVGGHCMPCFCFGITKKCQSTGRYRNQITLRFTEEEDFKGVNVSFPSRPGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDSYGGYLKFKVSYSLQRGGSEPKVRPDVVLTGSGQKFIY----RRGEPTFPD---TFNSREIRFTE-ENW--EHSNGRAVTRDDLMMTLANLESIHIRTIYDNRMASVGLSDISMDTTTKEFSQHGN----PREVEECRCPAGYSGLSCESCSPGFERVPGPYLGICAGCNCNGHASACDPVSGHCLSCQHKTEGAQCEKCKPGFFGDPSRGQYDDCKPCPCPYTETSRRFSDTCFLDHDNQATCDACREGYTGRR-----CEKC 843 HSP 2 Score: 55.0694 bits (131), Expect = 5.945e-62 Identity = 76/329 (23.10%), Postives = 126/329 (38.30%), Query Frame = -3
Query: 5301 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEYNT-------DLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVK----DVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6212
C++ GT N + C CK N G C EC G F + CL+CFC T++C S T + ++ V N Q+ + A NT RT + + +R ++ VY + + + +DI + L YGG++ + Y D D+V F+E S+ + T+ F E WR +G+ RE + AL I + + + ++++ D+ + H+ + VE C CP+G G C+ C+ VGY P + G C+ +C
Sbjct: 868 CDNRGTINPISKPCYCKSNVAGALCDECKTGAFHLTADNPEGCLQCFCMGVTKQCASS--------TWSRDQVRGGV---NGQLFTLSNAANT-------RTISEGITQRGSTEV--VYRSF----TNIHNDIFYWVLPQNFRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQGSGIFLEHYSQ-------IKPLPRVPATITVTFRES---AWRRA-DGQPCT-REHLLMALADINVFMIRATYADNMAETSLSNIQM-DIAVPHSTGNE---RAMEVEECACPQGYSGPSCQDCD-VGYTRT-------PGLYLGTCEKCEC 1148 HSP 3 Score: 73.559 bits (179), Expect = 1.458e-27 Identity = 92/372 (24.73%), Postives = 142/372 (38.17%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKC----TGRERNDPLCDKC 7356
C+ F+ A CL+CFC G T C+S+ + + L ++ I E + +V +S+ + +V+ N G + +Y LK +R ++ + P V+LQ G ++ + + P + S W + + R +L L+DI +IR Y N N +D I P S GN A +E+C CP Y+G SC+ C GY + C K +C + CD + G+C C G +C C G+ YG + D C C C Q +N S C L G VC+C TGR C++C
Sbjct: 895 CKTGAFHLTADNPEGCLQCFCMGVTKQCASSTWSRDQVRGGVNGQLFTLSNAANTRTISEGITQRGSTEVVYRSFTNIHNDIFYWVLPQN--------FRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQ---GSGIFLEHYSQIKPLPRVPATITVTFRESAWRRADG-------QPCTREHLLMALADINVFMIRATYADNMAETSLSNIQMD--IAVPHSTGNE-RAMEVEECACPQGYSGPSCQDCDVGYTRTPGLYLGTCEKCECHGHASSCDSETGDCLNCLHNTIGRRCENCISGY---YGNPKTGDPQACRPCPCLGQTSNQPSS--CY--LGSDGQPVCECPAGYTGRR-----CERC 1233 HSP 4 Score: 72.4034 bits (176), Expect = 1.458e-27 Identity = 76/292 (26.03%), Postives = 112/292 (38.36%), Query Frame = -3
Query: 5385 CNSLGTENIVD-GKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVN-RTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEY----NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFME------DNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6212
C+ G+E G C+CK N +GP CS C G F + CL CFC TQ+C S YR T+ S L NRQ +N V TE L N N +G +L + D + YGGK+ +T Y ++D + I + +++ +W +++ E +F E D P R L + + IRA + H + +G DV + + T L + VE C CP G G+ C+ C
Sbjct: 1259 CDQSGSEGCSGTGVCRCKMNVEGPTCSSCKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQ-----RTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQGDKVGA----YGGKLKYTISYVPGPRGSPIEDADVQIIGNDITLVARQ---IWQRGHGARESQDFEIVFREEHWQRPDGMPATREHLMMVLADLDDILIRA---SYHTEMRSSSISGVHMDVAVPEYT--------SLAQALEVEQCQCPPGYSGLSCQDC 1527 HSP 5 Score: 67.3958 bits (163), Expect = 3.658e-10 Identity = 85/385 (22.08%), Postives = 132/385 (34.29%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGY------------YQSPNPKTSTRTKGLLMNFK---SQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEV---IYSNPEGFYNQLKISL-WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C++ F+ + CL CFC G T C+S+ Y YQ RT + F S + ++SY Y Y + K YQ G + +Y LK I +PGP+ + + ND + V I+ G + + +E+W A R ++ VL+D+ +LIR Y++ +D + S+ + +E+C CP Y+GLSC+ C +GY + + C EC+ + G C C G C +C GF DC C C S C + G G C C++C
Sbjct: 1287 CKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQRTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQ-----------------GDKVGAYGGKLKYTISY-------VPGPRG--SPIEDADVQIIGNDITLVARQIWQRGHGARESQDFEIVFREEHW--QRPDGMPATREHLMMVLADLDDILIRASYHTEMRSSSISGVHMDVAVPEYTSLAQAL---EVEQCQCPPGYSGLSCQDCASGYTRTGGGLYLGHC--EPCECNGHSDTCHPETGICTNCQHNTKGELCEQCVDGFFGDPTAGTPEDCQPCACPGTDPENQFSRTC--ESLGNGGYQCTACQPGYLGQYCERC 1636 HSP 6 Score: 55.4546 bits (132), Expect = 1.874e-6 Identity = 66/267 (24.72%), Postives = 115/267 (43.07%), Query Frame = -3
Query: 5361 CSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLN---SADIK-LDVVLSNRQISGVYEAQNTNNQLLVNRTENIILV---KRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEI--ESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIE-HAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENV 6131
C G FS C+ CFC T++C+ + +YR + +TL D K ++V +R G+ + ++ L+ TE LV +R +Q + + + V YGG + F Y+ L + V P + + +KF +F + + E F E+N+ G+ + R+ + L + + I +DN + V L D++++ K + G VE C CP G G+ CE+C+ G+E V
Sbjct: 496 CRDGHFSVGGHCMPCFCFGITKKCQSTGRYR-NQITLRFTEEEDFKGVNVSFPSR--PGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDS--------YGGYLKFKVSYS--LQRGGSEPKVRPDVVLTGSGQKFIYRRGEPTFPDTFNSREIRFTEENWEH----SNGRAVT-RDDLMMTLANLESIHIRTIYDN-RMASVGLSDISMDTTTKEFSQHGNPREVEECRCPAGYSGLSCESCSP-GFERV 742
BLAST of SMED30010499 vs. Ensembl Zebrafish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 205.682 bits (522), Expect = 7.437e-62 Identity = 168/616 (27.27%), Postives = 263/616 (42.69%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFT-VHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEK--PMFNNAAMFVS------------MCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCK--CTGRERNDPLCDKC 8040
C +Q C+N CI C+G DC DG DE T + CEPN+F C + +C WRCDG+NDC + DE+NC K C F C + IP S CDE P+C + DE C P V + +E+ + +++ C + VP I WR NWG +P T + + G+ LTI ++ G YTCEA++ K + + I +L +K P+ +++ ++ C+ CFC G T C S Y + + KG+ ++F S+ L + + +N ++ +F +D + F + G+ ++SY YLK + + P P V+L K +Y E + + +N +I E ENW + + + R D++ L++++ + IR Y + + ++D GN PR +E+C CP Y+GLSCE C G+E+ C C + CD +G+C C + G +C +CKPGF DC C C + S+ C D Q C+ TGR C+KC
Sbjct: 199 CRADQATCQNGQCISREYLCDGESDCTDGSDESRCGTPSPCEPNEFKCQ-------NGRCALKLWRCDGDNDCQDNSDEMNCPTKRPGAACAPEQFVCLSDRKCIPASYQCDEEPDCADRSDEFGCAPPTVTVSPEESITASRGD-TVTFTCSAIGVPVPIITWRLNWGHIPANNRITMISENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLV---FAIPDGVLSLSQKPNPVVSSSPFILAGNCRDGHFSVGGHCMPCFCFGITKKCQSTGRYRNQITLRFTEEEDFKGVNVSFPSRPGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDSYGGYLKFKVSYSLQRGGSEPKVRPDVVLTGSGQKFIY----RRGEPTFPD---TFNSREIRFTE-ENW--EHSNGRAVTRDDLMMTLANLESIHIRTIYDNRMASVGLSDISMDTTTKEFSQHGN----PREVEECRCPAGYSGLSCESCSPGFERVPGPYLGICAGCNCNGHASACDPVSGHCLSCQHKTEGAQCEKCKPGFFGDPSRGQYDDCKPCPCPYTETSRRFSDTCFLDHDNQATCDACREGYTGRR-----CEKC 778 HSP 2 Score: 55.4546 bits (132), Expect = 7.437e-62 Identity = 76/329 (23.10%), Postives = 126/329 (38.30%), Query Frame = -3
Query: 5301 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEYNT-------DLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVK----DVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6212
C++ GT N + C CK N G C EC G F + CL+CFC T++C S T + ++ V N Q+ + A NT RT + + +R ++ VY + + + +DI + L YGG++ + Y D D+V F+E S+ + T+ F E WR +G+ RE + AL I + + + ++++ D+ + H+ + VE C CP+G G C+ C+ VGY P + G C+ +C
Sbjct: 803 CDNRGTINPISKPCYCKSNVAGALCDECKTGAFHLTADNPEGCLQCFCMGVTKQCASS--------TWSRDQVRGGV---NGQLFTLSNAANT-------RTISEGITQRGSTEV--VYRSF----TNIHNDIFYWVLPQNFRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQGSGIFLEHYSQ-------IKPLPRVPATITVTFRES---AWRRA-DGQPCT-REHLLMALADINVFMIRATYADNMAETSLSNIQM-DIAVPHSTGNE---RAMEVEECACPQGYSGPSCQDCD-VGYTRT-------PGLYLGTCEKCEC 1083 HSP 3 Score: 73.559 bits (179), Expect = 1.610e-27 Identity = 92/372 (24.73%), Postives = 142/372 (38.17%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKC----TGRERNDPLCDKC 7356
C+ F+ A CL+CFC G T C+S+ + + L ++ I E + +V +S+ + +V+ N G + +Y LK +R ++ + P V+LQ G ++ + + P + S W + + R +L L+DI +IR Y N N +D I P S GN A +E+C CP Y+G SC+ C GY + C K +C + CD + G+C C G +C C G+ YG + D C C C Q +N S C L G VC+C TGR C++C
Sbjct: 830 CKTGAFHLTADNPEGCLQCFCMGVTKQCASSTWSRDQVRGGVNGQLFTLSNAANTRTISEGITQRGSTEVVYRSFTNIHNDIFYWVLPQN--------FRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQ---GSGIFLEHYSQIKPLPRVPATITVTFRESAWRRADG-------QPCTREHLLMALADINVFMIRATYADNMAETSLSNIQMD--IAVPHSTGNE-RAMEVEECACPQGYSGPSCQDCDVGYTRTPGLYLGTCEKCECHGHASSCDSETGDCLNCLHNTIGRRCENCISGY---YGNPKTGDPQACRPCPCLGQTSNQPSS--CY--LGSDGQPVCECPAGYTGRR-----CERC 1168 HSP 4 Score: 72.4034 bits (176), Expect = 1.610e-27 Identity = 76/292 (26.03%), Postives = 112/292 (38.36%), Query Frame = -3
Query: 5385 CNSLGTENIVD-GKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVN-RTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEY----NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFME------DNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6212
C+ G+E G C+CK N +GP CS C G F + CL CFC TQ+C S YR T+ S L NRQ +N V TE L N N +G +L + D + YGGK+ +T Y ++D + I + +++ +W +++ E +F E D P R L + + IRA + H + +G DV + + T L + VE C CP G G+ C+ C
Sbjct: 1194 CDQSGSEGCSGTGVCRCKMNVEGPTCSSCKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQ-----RTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQGDKVGA----YGGKLKYTISYVPGPRGSPIEDADVQIIGNDITLVARQ---IWQRGHGARESQDFEIVFREEHWQRPDGMPATREHLMMVLADLDDILIRA---SYHTEMRSSSISGVHMDVAVPEYT--------SLAQALEVEQCQCPPGYSGLSCQDC 1462 HSP 5 Score: 67.3958 bits (163), Expect = 4.007e-10 Identity = 85/385 (22.08%), Postives = 132/385 (34.29%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGY------------YQSPNPKTSTRTKGLLMNFK---SQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEV---IYSNPEGFYNQLKISL-WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C++ F+ + CL CFC G T C+S+ Y YQ RT + F S + ++SY Y Y + K YQ G + +Y LK I +PGP+ + + ND + V I+ G + + +E+W A R ++ VL+D+ +LIR Y++ +D + S+ + +E+C CP Y+GLSC+ C +GY + + C EC+ + G C C G C +C GF DC C C S C + G G C C++C
Sbjct: 1222 CKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQRTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQ-----------------GDKVGAYGGKLKYTISY-------VPGPRG--SPIEDADVQIIGNDITLVARQIWQRGHGARESQDFEIVFREEHW--QRPDGMPATREHLMMVLADLDDILIRASYHTEMRSSSISGVHMDVAVPEYTSLAQAL---EVEQCQCPPGYSGLSCQDCASGYTRTGGGLYLGHC--EPCECNGHSDTCHPETGICTNCQHNTKGELCEQCVDGFFGDPTAGTPEDCQPCACPGTDPENQFSRTC--ESLGNGGYQCTACQPGYLGQYCERC 1571 HSP 6 Score: 55.4546 bits (132), Expect = 1.665e-6 Identity = 66/267 (24.72%), Postives = 115/267 (43.07%), Query Frame = -3
Query: 5361 CSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLN---SADIK-LDVVLSNRQISGVYEAQNTNNQLLVNRTENIILV---KRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEI--ESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIE-HAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENV 6131
C G FS C+ CFC T++C+ + +YR + +TL D K ++V +R G+ + ++ L+ TE LV +R +Q + + + V YGG + F Y+ L + V P + + +KF +F + + E F E+N+ G+ + R+ + L + + I +DN + V L D++++ K + G VE C CP G G+ CE+C+ G+E V
Sbjct: 431 CRDGHFSVGGHCMPCFCFGITKKCQSTGRYR-NQITLRFTEEEDFKGVNVSFPSR--PGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDS--------YGGYLKFKVSYS--LQRGGSEPKVRPDVVLTGSGQKFIYRRGEPTFPDTFNSREIRFTEENWEH----SNGRAVT-RDDLMMTLANLESIHIRTIYDN-RMASVGLSDISMDTTTKEFSQHGNPREVEECRCPAGYSGLSCESCSP-GFERV 677
BLAST of SMED30010499 vs. Ensembl Zebrafish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 205.682 bits (522), Expect = 8.147e-62 Identity = 168/616 (27.27%), Postives = 263/616 (42.69%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFT-VHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEK--PMFNNAAMFVS------------MCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCK--CTGRERNDPLCDKC 8040
C +Q C+N CI C+G DC DG DE T + CEPN+F C + +C WRCDG+NDC + DE+NC K C F C + IP S CDE P+C + DE C P V + +E+ + +++ C + VP I WR NWG +P T + + G+ LTI ++ G YTCEA++ K + + I +L +K P+ +++ ++ C+ CFC G T C S Y + + KG+ ++F S+ L + + +N ++ +F +D + F + G+ ++SY YLK + + P P V+L K +Y E + + +N +I E ENW + + + R D++ L++++ + IR Y + + ++D GN PR +E+C CP Y+GLSCE C G+E+ C C + CD +G+C C + G +C +CKPGF DC C C + S+ C D Q C+ TGR C+KC
Sbjct: 199 CRADQATCQNGQCISREYLCDGESDCTDGSDESRCGTPSPCEPNEFKCQ-------NGRCALKLWRCDGDNDCQDNSDEMNCPTKRPGAACAPEQFVCLSDRKCIPASYQCDEEPDCADRSDEFGCAPPTVTVSPEESITASRGD-TVTFTCSAIGVPVPIITWRLNWGHIPANNRITMISENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLV---FAIPDGVLSLSQKPNPVVSSSPFILAGNCRDGHFSVGGHCMPCFCFGITKKCQSTGRYRNQITLRFTEEEDFKGVNVSFPSRPGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDSYGGYLKFKVSYSLQRGGSEPKVRPDVVLTGSGQKFIY----RRGEPTFPD---TFNSREIRFTE-ENW--EHSNGRAVTRDDLMMTLANLESIHIRTIYDNRMASVGLSDISMDTTTKEFSQHGN----PREVEECRCPAGYSGLSCESCSPGFERVPGPYLGICAGCNCNGHASACDPVSGHCLSCQHKTEGAQCEKCKPGFFGDPSRGQYDDCKPCPCPYTETSRRFSDTCFLDHDNQATCDACREGYTGRR-----CEKC 778 HSP 2 Score: 55.4546 bits (132), Expect = 8.147e-62 Identity = 76/329 (23.10%), Postives = 126/329 (38.30%), Query Frame = -3
Query: 5301 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEYNT-------DLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVK----DVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6212
C++ GT N + C CK N G C EC G F + CL+CFC T++C S T + ++ V N Q+ + A NT RT + + +R ++ VY + + + +DI + L YGG++ + Y D D+V F+E S+ + T+ F E WR +G+ RE + AL I + + + ++++ D+ + H+ + VE C CP+G G C+ C+ VGY P + G C+ +C
Sbjct: 803 CDNRGTINPISKPCYCKSNVAGALCDECKTGAFHLTADNPEGCLQCFCMGVTKQCASS--------TWSRDQVRGGV---NGQLFTLSNAANT-------RTISEGITQRGSTEV--VYRSF----TNIHNDIFYWVLPQNFRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQGSGIFLEHYSQ-------IKPLPRVPATITVTFRES---AWRRA-DGQPCT-REHLLMALADINVFMIRATYADNMAETSLSNIQM-DIAVPHSTGNE---RAMEVEECACPQGYSGPSCQDCD-VGYTRT-------PGLYLGTCEKCEC 1083 HSP 3 Score: 73.1738 bits (178), Expect = 1.520e-27 Identity = 92/372 (24.73%), Postives = 142/372 (38.17%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKC----TGRERNDPLCDKC 7356
C+ F+ A CL+CFC G T C+S+ + + L ++ I E + +V +S+ + +V+ N G + +Y LK +R ++ + P V+LQ G ++ + + P + S W + + R +L L+DI +IR Y N N +D I P S GN A +E+C CP Y+G SC+ C GY + C K +C + CD + G+C C G +C C G+ YG + D C C C Q +N S C L G VC+C TGR C++C
Sbjct: 830 CKTGAFHLTADNPEGCLQCFCMGVTKQCASSTWSRDQVRGGVNGQLFTLSNAANTRTISEGITQRGSTEVVYRSFTNIHNDIFYWVLPQN--------FRGDKVTAYGGELKYRVRYEPHARSMVIDGQPDVVLQ---GSGIFLEHYSQIKPLPRVPATITVTFRESAWRRADG-------QPCTREHLLMALADINVFMIRATYADNMAETSLSNIQMD--IAVPHSTGNE-RAMEVEECACPQGYSGPSCQDCDVGYTRTPGLYLGTCEKCECHGHASSCDSETGDCLNCLHNTIGRRCENCISGY---YGNPKTGDPQACRPCPCLGQTSNQPSS--CY--LGSDGQPVCECPAGYTGRR-----CERC 1168 HSP 4 Score: 72.7886 bits (177), Expect = 1.520e-27 Identity = 76/292 (26.03%), Postives = 112/292 (38.36%), Query Frame = -3
Query: 5385 CNSLGTENIVD-GKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVN-RTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEY----NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFME------DNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6212
C+ G+E G C+CK N +GP CS C G F + CL CFC TQ+C S YR T+ S L NRQ +N V TE L N N +G +L + D + YGGK+ +T Y ++D + I + +++ +W +++ E +F E D P R L + + IRA + H + +G DV + + T L + VE C CP G G+ C+ C
Sbjct: 1194 CDQSGSEGCSGTGVCRCKMNVEGPTCSSCKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQ-----RTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQGDKVGA----YGGKLKYTISYVPGPRGSPIEDADVQIIGNDITLVARQ---IWQRGHGARESQDFEIVFREEHWQRPDGMPATREHLMMVLADLDDILIRA---SYHTEMRSSSISGVHMDVAVPEYT--------SLAQALEVEQCQCPPGYSGLSCQDC 1462 HSP 5 Score: 67.0106 bits (162), Expect = 4.938e-10 Identity = 85/385 (22.08%), Postives = 132/385 (34.29%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGY------------YQSPNPKTSTRTKGLLMNFK---SQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEV---IYSNPEGFYNQLKISL-WEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C++ F+ + CL CFC G T C+S+ Y YQ RT + F S + ++SY Y Y + K YQ G + +Y LK I +PGP+ + + ND + V I+ G + + +E+W A R ++ VL+D+ +LIR Y++ +D + S+ + +E+C CP Y+GLSC+ C +GY + + C EC+ + G C C G C +C GF DC C C S C + G G C C++C
Sbjct: 1222 CKQGTFHLSPANKDGCLSCFCMGVTQQCTSSRHYRDVVSTMFSPGNYQGFALVNRQRTNRIDNGFSVELSTEGTQLSYTNFNYLGQEPHYWQLPKAYQ-----------------GDKVGAYGGKLKYTISY-------VPGPRG--SPIEDADVQIIGNDITLVARQIWQRGHGARESQDFEIVFREEHW--QRPDGMPATREHLMMVLADLDDILIRASYHTEMRSSSISGVHMDVAVPEYTSLAQAL---EVEQCQCPPGYSGLSCQDCASGYTRTGGGLYLGHC--EPCECNGHSDTCHPETGICTNCQHNTKGELCEQCVDGFFGDPTAGTPEDCQPCACPGTDPENQFSRTC--ESLGNGGYQCTACQPGYLGQYCERC 1571 HSP 6 Score: 55.4546 bits (132), Expect = 1.623e-6 Identity = 66/267 (24.72%), Postives = 115/267 (43.07%), Query Frame = -3
Query: 5361 CSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLN---SADIK-LDVVLSNRQISGVYEAQNTNNQLLVNRTENIILV---KRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEI--ESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIE-HAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENV 6131
C G FS C+ CFC T++C+ + +YR + +TL D K ++V +R G+ + ++ L+ TE LV +R +Q + + + V YGG + F Y+ L + V P + + +KF +F + + E F E+N+ G+ + R+ + L + + I +DN + V L D++++ K + G VE C CP G G+ CE+C+ G+E V
Sbjct: 431 CRDGHFSVGGHCMPCFCFGITKKCQSTGRYR-NQITLRFTEEEDFKGVNVSFPSR--PGLPQPLSSTQMLINPETEEFQLVDLSRRFLDQDSFWSLPRQFLGNKVDS--------YGGYLKFKVSYS--LQRGGSEPKVRPDVVLTGSGQKFIYRRGEPTFPDTFNSREIRFTEENWEH----SNGRAVT-RDDLMMTLANLESIHIRTIYDN-RMASVGLSDISMDTTTKEFSQHGNPREVEECRCPAGYSGLSCESCSP-GFERV 677
BLAST of SMED30010499 vs. Ensembl Zebrafish
Match: lama1 (laminin, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-060118-1]) HSP 1 Score: 79.337 bits (194), Expect = 2.705e-16 Identity = 86/381 (22.57%), Postives = 156/381 (40.94%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKI----SLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQ-------------CVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYG--EKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C+ F +A+ + C CFC G +D C G ++P S+ L+ SQ L+ + + +Y + + V ++ + ++ + +G+ L SY L + F + + P+V+L+ + + L VIY++ N ++ +L E + + +S + + D ++VLS+++Y++I+ Y S N +++ + + A +E C CP Y GLSC++C GY + ++ CV +C + CD G C C +G C C G+ YG + SDC+ C C L S+ C+ L G G C C++C
Sbjct: 1134 CKSGSFGLSALNPAGCSPCFCFGLSDVCEELSGLVRAPIVLGSSPA---LLRVVSQSDLQGTL-EGVYYSDDEMLLDVDQLNKLSLLTGPYYWRLPQKYNGNKLLSYGGRLSYTLTFFAQDGVGLANQEPQVLLRGGHLRKL---------VIYTDASAPANGIRSTQEHALTEHKWKYFNSVSDEAVSHADFMSVLSNLEYVIIKASYGSGLQQSRISNISMETALEADELPEGGDVARLVEICECPPGYAGLSCQECAPGYYRQAVSELNMKGRNRPLIQPCVPCRCSNHSQSCDLHTGQCLGCQHNTAGEHCHVCAAGY---YGKVQGSVSDCSLCTC--PLRGQSFSSTCV--LEGAGDFRCDRCEEGYEGRYCERC 1494 HSP 2 Score: 28.8758 bits (63), Expect = 2.705e-16 Identity = 9/31 (29.03%), Postives = 16/31 (51.61%), Query Frame = -3
Query: 6087 GKCKCKENYKGPNCSECSPGFFSFQKKCLKC 6179
G+C+CK +G C +C ++C+ C
Sbjct: 1529 GRCECKPGVRGHLCDQCEERHVLVHEQCVSC 1559 HSP 3 Score: 78.9518 bits (193), Expect = 1.308e-13 Identity = 87/358 (24.30%), Postives = 144/358 (40.22%), Query Frame = -2
Query: 6328 LCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLT--SDFGMDGSWLESYSYYLKINIRLFGESKDDIPGP-----KVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNI--YAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVC 7359
LC++ +N A C +CFC G +D C S+ + S G+L I S+ + D N + D DP+ T + + L SY +L ++ + S++++ VI++ NG++L S + N + ++ + FIDS S R +++ VL+D+ L +R + + G + HV +++ + +E C CP Y+G SCE C+ G+ + C+ +C + ECD NG C C +GP C +C PGF E DC C+C L + S C L G +C
Sbjct: 490 LCKQGFYNLQASNPEGCTECFCFGVSDVCESSTWFSSS-----VVHRDGVLHRLHQTSI---SFWSPVSDEN----LIISNRSTD--ADPSSVWTWAAPEPFLNNKLTSYGSFLNYSV-AYDTSEENVDKTLRSHFSVIIEG-NGRTLRQA---QSARLLLNA---HTHQRVFVQMLPQIFIDSHSGRSVQRDELMTVLADVAALRVRAELEDSAEG----TLRLSHVSLGVGDSNSDVSKISLDVEHCECPWGYSGTSCELCIPGFYRVGGILFGGNCLPCECNDHATECDINGECLGCAHNTTGPHCDQCLPGFYGDASEGTPDDCQRCSCPLTLASNNFSPTCQ--LQAPGEFIC 819 HSP 4 Score: 51.2174 bits (121), Expect = 2.321e-11 Identity = 69/294 (23.47%), Postives = 124/294 (42.18%), Query Frame = -3
Query: 5355 GKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEA-QNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLL-YGGKISFTFEYNTDLLKDLV-FSDVTPFIEIESKKFGRL--WTVLS--FNEKTKTMESLFMEDNFPKWR----IGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDL---GKIAP-VEMCLCPKGNPGIKCEACNKVGYENVIN 6179
G+C CK+N G C EC G F C CFC + C+E + + L S+ L VV S + G E +++++L+ + +Q+ + + +P + LL YGG++S+T + +D V ++ P + + +L +T S N T E E KW+ + E D F+ L +++ I + +G ++ R+ ++++E A ++L G +A VE+C CP G G+ C+ C Y ++
Sbjct: 1118 GQCSCKDNVGGRGCDECKSGSFGLSALNPAGCSPCFCFGLSDVCEELSGLVRAPIVLGSSPALLRVV-SQSDLQGTLEGVYYSDDEMLL-----------DVDQLNKLSLLTGPYYWRLPQKYNGNKLLSYGGRLSYTLTF---FAQDGVGLANQEPQVLLRGGHLRKLVIYTDASAPANGIRSTQEHALTEH---KWKYFNSVSDEAVSHAD---FMSVLSNLEYVIIKASYGSG-LQQSRISNISMETALEADELPEGGDVARLVEICECPPGYAGLSCQECAPGYYRQAVS 1389 HSP 5 Score: 40.0466 bits (92), Expect = 2.321e-11 Identity = 40/131 (30.53%), Postives = 51/131 (38.93%), Query Frame = -2
Query: 6283 KCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQ------RSGPKCLECKPGFVRLYGEKLSSDCTECNCYS----QLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6645
+C C T TG C++C GY +N C C +GNCN TG+ G KC +C G +G S C CNC + L S C+ L G +C RN P C C
Sbjct: 971 RCQCITGVTGDKCDRCHHGYYNFKENGCTA-----CDCAHTHGNCNDQTGECICPPHTHGLKCEQCDEGH---WGHDGVSGCKVCNCSAVGSSSSQCDLSSGQCVCALQFSGLTCDRCALGYRNFPQCSAC 1093 HSP 6 Score: 46.2098 bits (108), Expect = 1.535e-8 Identity = 44/143 (30.77%), Postives = 60/143 (41.96%), Query Frame = -2
Query: 6241 CPTPYTGLSCEKCLAGY--EKNDQNQCVKKC-------PINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSS-DCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCK 6636
C YTG C++C GY E Q C P CD + G C +C G +GP C C+ G+ YG+ + +C C CYS N +S IC +TGQ C+C CD+C + I C+
Sbjct: 822 CQPGYTGDKCQRCADGYFGEPAVPGQRCSPCECNGNVDPSEAGRCDTHTGECLKCVGHTAGPHCERCRDGY---YGDAIQEKNCQACGCYS---NGSLSTIC-NLITGQ----CECK-HNVVGKTCDRCQEGYHGINSGEGCR 952 HSP 7 Score: 35.039 bits (79), Expect = 1.535e-8 Identity = 12/44 (27.27%), Postives = 23/44 (52.27%), Query Frame = -3
Query: 6054 DGKCKCKENYKGPNCSECSPGFFSFQKK-CLKCFCSNKTQECKE 6182
+G+C+C G C C G+++F++ C C C++ C +
Sbjct: 969 EGRCQCITGVTGDKCDRCHHGYYNFKENGCTACDCAHTHGNCND 1012
BLAST of SMED30010499 vs. Ensembl Zebrafish
Match: lrp1ba (low density lipoprotein receptor-related protein 1Ba [Source:ZFIN;Acc:ZDB-GENE-050208-608]) HSP 1 Score: 86.6557 bits (213), Expect = 5.854e-16 Identity = 52/130 (40.00%), Postives = 62/130 (47.69%), Query Frame = -2
Query: 7678 TLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8061
T + C +NQ AC + CI + C+G DC DG DE C A C P FSC P CV+ W CDGE DC +G DEL NTC S F C N IPR CD + +C + DE
Sbjct: 2659 TELTNSSCLWNQFACSKNKCISKQWLCDGEDDCGDGLDESQEICGSASCSPGLFSC------PGSYACVSRNWLCDGERDCPDGSDELALAGSPNNTCTDSTFQC-RNQNCIPRRFVCDHDDDCGDASDE 2781 HSP 2 Score: 62.3882 bits (150), Expect = 1.291e-8 Identity = 39/131 (29.77%), Postives = 56/131 (42.75%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-------EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS Q C + CI E+ C+G DC D DE I+ C +F C G C+ WRCDG+ DC +G DE C + + F C + + + CD + +C + DE+ C
Sbjct: 983 CSVAQFQCSSGKCIPEHWMCDGDNDCGDLSDENATCSRTAISAINDCSEKEFHCRGDG------TCIPEIWRCDGDKDCEDGSDEFACEGAKRMCDPKAKFTCKVSGKCVSKDWVCDGDIDCEDQSDEEGC 1107 HSP 3 Score: 58.5362 bits (140), Expect = 1.905e-7 Identity = 43/158 (27.22%), Postives = 61/158 (38.61%), Query Frame = -2
Query: 7678 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPP----------------------------------HQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8043
C +Q C N+ CI + C+G DC DE TC+ C+P QF C P + KC++ W CD E+DCG+G DE+ C+ + C + F C IP CD + +C + DE
Sbjct: 862 ICPADQFKCLNNRCIPKRWLCDGTDDCGSNEDESNRTCSAQTCQPGQFPCANGRCIPENWRCDRDDDCGDQSDESSSCDFPTCEPLTQFGCTNGKCISVKWHCDSEDDCGDGSDEVGCV----HACSVAQFQCSSG-KCIPEHWMCDGDNDCGDLSDE 1014
BLAST of SMED30010499 vs. Ensembl Xenopus
Match: sel1l (SEL1L adaptor subunit of ERAD E3 ubiquitin ligase [Source:Xenbase;Acc:XB-GENE-973992]) HSP 1 Score: 209.92 bits (533), Expect = 1.605e-60 Identity = 174/605 (28.76%), Postives = 253/605 (41.82%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFN---NAAMFV---SMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQ-KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C ++ C N CI + C+G KDC+DG DE T + CEPN+F C + +C WRCDG+NDCG+G DELNC K +TC F C T IP S CD+ +C + DE C P+V+ P +E+ + ++ C + VPT I WR NWG +P + V T ++ + L I ++ G YTCEA++ AK + L KP + +V + C+ CFC G T C Y + KG+ S I +S + D + + ++ V + +D L F G+ ++SY L +R L + + P VIL NG+ L + V S NQ KI E ENW +S S R D+L L +++ ++I+ Y + + +D +G + +E+C CP Y G SCE C A +E+ + C NC CD +G C C GP+C +CK GF +S C C C S+ C D+ GQ P CD C
Sbjct: 252 CRRDEARCPNGQCIPRDYLCDGEKDCKDGSDEMHCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCGDGSDELNCPTKGPSDTCAPDQFVCVQARTCIPASYQCDDEADCPDRSDEVGCNPPQVITPPEESIMASRGD-TVRFTCVAIGVPTPIITWRLNWGHIP--TSSRVSMTSENGH---GTLIIRDVKEADQGAYTCEAIN-----AKGMVFGIPDGVLILKPHRGPCPEGSFYVENSARCIPCFCFGVTKVCQQTGRYRNQIRLRFDVPEDFKGVNFTVPSHVGIPPLSSNQLQIDTSVEEFQLVDLSRRFLSIDSFWTLPGQF--LGNKVDSYGGSLSFKVRYGLTRGQSEPVRKPDVILVG-NGRKLIY------RVQTSTQPSIVNQRKIQFTE-ENWQHESG--ASVTREDLLMTLQNLEAIMIQTVYDNKMASVGISDIVMDTTSVEYTQLGPAV---GVEECRCPGGYAGPSCEVCAAQFERVPGGPFLGTCSGCNCNGHASSCDPVSGYCLNCQHNTEGPQCNKCKAGFFGDPTRGVSDACRPCPCPHTDPRRRFSDTCFMDVDGQ--------------PTCDSC 809 HSP 2 Score: 47.3654 bits (111), Expect = 1.605e-60 Identity = 68/293 (23.21%), Postives = 109/293 (37.20%), Query Frame = -3
Query: 5385 GKCK----CKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNR-QISGVYEAQNTNNQLLVNRT--ENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFP----KWRI--GKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6179
G+C+ + + +G C+EC G F +K CLKCFC T++C S NR Q+SG Y T + RT E+I R Q L + VP D++ + L YGG++ +T + +F P + ++ L + T+T + P WR G++ RE + AL L I + V + R+ ++ ++ A + D + VE C+CP G G C+ C
Sbjct: 834 GRCEPPLLSQSHVEGNFCNECKSGTFYLSEKNPDGCLKCFCMGITRQCSSS--------------------FWNRDQVSGCYRVPFTISNAGSTRTLSESI----RVTGQSELTFSNF----LNVPQDVYFWVLPDRFKGDKVTSYGGELRYTITFEAPAGSQPLFGQ--PDVVLQGNGI-----FLEHSANTRTAPGFPITITVPFRERAWRRADGQDAT----REHLLMALADIDLLMIRASYAERMV-ETRISNIHMDVAVPYPTDRQRAVEVEHCVCPPGYSGPSCQVC 1086 HSP 3 Score: 62.3882 bits (150), Expect = 2.031e-8 Identity = 72/325 (22.15%), Postives = 120/325 (36.92%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQ---SPNPKTSTRTKGLLMNFKSQKI---LEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYS----YYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSN------PEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PINC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
CL CFC G T C+S+ + + +P + + L+ +S +I V ++ + S+ +G+ + + G G + SY Y L N + G + D + ND + V Y + + F + S W++ + A R ++ L+D+ +L+R Y ++ +++ T S N A +E+C CP Y GLSC+ C GY + + C P C C + G C+ C G C +C PGF DC C C
Sbjct: 1233 CLPCFCMGVTQQCTSSTYHRKLISTPFLPGNFQNFALVNRQRSSRITSGFTVEMSRHGPQLTHSSFDQLGQE--------SYYWQLPEGYQGDKVASYGGKLRYTLTYNAGIRGSALPD-------------ADVQITGNDITLVAYQHDLRPRESKSFEIVFRESSWKRPDG-------QPATREHLMMALADLDEILVRASYSTDMLSSSITGVSME---TAGPSYTNLPQALEVEECRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEPCYCNGHSESCHPETGACSNCLHNTVGESCDQCAPGFYGDASAGTPEDCQPCAC 1526 HSP 4 Score: 57.7658 bits (138), Expect = 4.257e-7 Identity = 62/284 (21.83%), Postives = 105/284 (36.97%), Query Frame = -3
Query: 5385 KCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTEN--------IILVKRNP-------NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6176
+C+CK N +G +C C P F + CL CFC TQ+C S +R + IS + N N LVNR + + + + P +Q+G +L D + YGGK+ +T YN + P +++ G T++++ + ES E F + + RE + AL + + + + + +++E A +L + VE C CP G G+ C+ C
Sbjct: 1203 QCECKPNVEGASCGTCRPHHFYLSTENPDGCLPCFCMGVTQQCTSSTYHR-------------------KLISTPFLPGNFQNFALVNRQRSSRITSGFTVEMSRHGPQLTHSSFDQLGQESYYWQLPEGYQGDKVAS----YGGKLRYTLTYNAG-----IRGSALPDADVQIT--GNDITLVAYQHDLRPRESKSFEIVFRESSWKRPDGQPATREHLMMALADLDEILVRASYSTDMLSS-SITGVSMETAGPSYTNLPQALEVEECRCPPGYQGLSCQDC 1455
BLAST of SMED30010499 vs. Ensembl Xenopus
Match: sel1l (SEL1L adaptor subunit of ERAD E3 ubiquitin ligase [Source:Xenbase;Acc:XB-GENE-973992]) HSP 1 Score: 211.846 bits (538), Expect = 1.840e-58 Identity = 174/605 (28.76%), Postives = 250/605 (41.32%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFN---NAAMFV---SMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQ-KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C ++ C N CI + C+G KDC+DG DE T + CEPN+F C + +C WRCDG+NDCG+G DELNC K +TC F C T IP S CD+ +C + DE C P+V+ P +E+ + ++ C + VPT I WR NWG +P + H T L I ++ G YTCEA++ AK + L KP + +V + C+ CFC G T C Y + KG+ S I +S + D + + ++ V + +D L F G+ ++SY L +R L + + P VIL NG+ L + V S NQ KI E ENW +S S R D+L L +++ ++I+ Y + + +D +G + +E+C CP Y G SCE C A +E+ + C NC CD +G C C GP+C +CK GF +S C C C S+ C D+ GQ P CD C
Sbjct: 195 CRRDEARCPNGQCIPRDYLCDGEKDCKDGSDEMHCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCGDGSDELNCPTKGPSDTCAPDQFVCVQARTCIPASYQCDDEADCPDRSDEVGCNPPQVITPPEESIMASRGD-TVRFTCVAIGVPTPIITWRLNWGHIPTSSSENGHGT----------LIIRDVKEADQGAYTCEAIN-----AKGMVFGIPDGVLILKPHRGPCPEGSFYVENSARCIPCFCFGVTKVCQQTGRYRNQIRLRFDVPEDFKGVNFTVPSHVGIPPLSSNQLQIDTSVEEFQLVDLSRRFLSIDSFWTLPGQF--LGNKVDSYGGSLSFKVRYGLTRGQSEPVRKPDVILVG-NGRKLIY------RVQTSTQPSIVNQRKIQFTE-ENWQHESG--ASVTREDLLMTLQNLEAIMIQTVYDNKMASVGISDIVMDTTSVEYTQLGPAV---GVEECRCPGGYAGPSCEVCAAQFERVPGGPFLGTCSGCNCNGHASSCDPVSGYCLNCQHNTEGPQCNKCKAGFFGDPTRGVSDACRPCPCPHTDPRRRFSDTCFMDVDGQ--------------PTCDSC 747 HSP 2 Score: 38.5058 bits (88), Expect = 1.840e-58 Identity = 18/51 (35.29%), Postives = 27/51 (52.94%), Query Frame = -3
Query: 6051 GKCK----CKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKES 6179
G+C+ + + +G C+EC G F +K CLKCFC T++C S
Sbjct: 772 GRCEPPLLSQSHVEGNFCNECKSGTFYLSEKNPDGCLKCFCMGITRQCSSS 822 HSP 3 Score: 62.3882 bits (150), Expect = 1.763e-8 Identity = 72/325 (22.15%), Postives = 120/325 (36.92%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQ---SPNPKTSTRTKGLLMNFKSQKI---LEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYS----YYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSN------PEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PINC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
CL CFC G T C+S+ + + +P + + L+ +S +I V ++ + S+ +G+ + + G G + SY Y L N + G + D + ND + V Y + + F + S W++ + A R ++ L+D+ +L+R Y ++ +++ T S N A +E+C CP Y GLSC+ C GY + + C P C C + G C+ C G C +C PGF DC C C
Sbjct: 1137 CLPCFCMGVTQQCTSSTYHRKLISTPFLPGNFQNFALVNRQRSSRITSGFTVEMSRHGPQLTHSSFDQLGQE--------SYYWQLPEGYQGDKVASYGGKLRYTLTYNAGIRGSALPD-------------ADVQITGNDITLVAYQHDLRPRESKSFEIVFRESSWKRPDG-------QPATREHLMMALADLDEILVRASYSTDMLSSSITGVSME---TAGPSYTNLPQALEVEECRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEPCYCNGHSESCHPETGACSNCLHNTVGESCDQCAPGFYGDASAGTPEDCQPCAC 1430
BLAST of SMED30010499 vs. Ensembl Xenopus
Match: sel1l (SEL1L adaptor subunit of ERAD E3 ubiquitin ligase [Source:Xenbase;Acc:XB-GENE-973992]) HSP 1 Score: 207.994 bits (528), Expect = 2.231e-58 Identity = 174/605 (28.76%), Postives = 254/605 (41.98%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFN---NAAMFV---SMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRT--KGLLMNFKSQ-KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C ++ C N CI + C+G KDC+DG DE T + CEPN+F C + +C WRCDG+NDCG+G DELNC K +TC F C T IP S CD+ +C + DE C P+V+ P +E+ + ++ C + VPT I WR NWG +P + V T ++ + L I ++ G YTCEA++ AK + L KP + +V + C+ CFC G T C Y + KG+ S I +S + D + + ++ V + +D F T G+ ++SY L +R L + + P VIL NG+ L + V S NQ KI E ENW +S S R D+L L +++ ++I+ Y + + +D +G + +E+C CP Y G SCE C A +E+ + C NC CD +G C C GP+C +CK GF +S C C C + S+ C D+ GQ P CD C
Sbjct: 219 CRRDEARCPNGQCIPRDYLCDGEKDCKDGSDEMHCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCGDGSDELNCPTKGPSDTCAPDQFVCVQARTCIPASYQCDDEADCPDRSDEVGCNPPQVITPPEESIMASRGD-TVRFTCVAIGVPTPIITWRLNWGHIP--TSSRVSMTSENGH---GTLIIRDVKEADQGAYTCEAIN-----AKGMVFGIPDGVLILKPHRGPCPEGSFYVENSARCIPCFCFGVTKVCQQTGRYRNQIRLRFDVPEDFKGVNFTVPSHVGIPPLSSNQLQIDTSVEEFQLVDLSRRFLSID--SFWTLPGQFLGNKVDSYGGSLSFKVRYGLTRGQSEPVRKPDVILVG-NGRKLIY------RVQTSTQPSIVNQRKIQFTE-ENWQHESG--ASVTREDLLMTLQNLEAIMIQTVYDNKMASVGISDIVMDTTSVEYTQLGPAV---GVEECRCPGGYAGPSCEVCAAQFERVPGGPFLGTCSGCNCNGHASSCDPVSGYCLNCQHNTEGPQCNKCKAGFFGDPTRGVSDACRPCPC--PHTDPRFSDTCFMDVDGQ--------------PTCDSC 774 HSP 2 Score: 41.9726 bits (97), Expect = 2.231e-58 Identity = 68/293 (23.21%), Postives = 109/293 (37.20%), Query Frame = -3
Query: 5385 GKCK----CKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNR-QISGVYEAQNTNNQLLVNRT--ENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFP----KWRI--GKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6179
G+C+ + + +G C+EC G F +K CLKCFC T++C S NR Q+SG Y T + RT E+I R Q L + VP D++ + L YGG++ +T + +F P + ++ L + T+T + P WR G++ RE + AL L I + V + R+ ++ ++ A + D + VE C+CP G G C+ C
Sbjct: 799 GRCEPPLLSQSHVEGNFCNECKSGTFYLSEKNPDGCLKCFCMGITRQCSSS--------------------FWNRDQVSG-YRVPFTISNAGSTRTLSESI----RVTGQSELTFSNF----LNVPQDVYFWVLPDRFKGDKVTSYGGELRYTITFEAPAGSQPLFGQ--PDVVLQGNGI-----FLEHSANTRTAPGFPITITVPFRERAWRRADGQDAT----REHLLMALADIDLLMIRASYAERMV-ETRISNIHMDVAVPYPTDRQRAVEVEHCVCPPGYSGPSCQVC 1050 HSP 3 Score: 62.3882 bits (150), Expect = 1.822e-8 Identity = 72/325 (22.15%), Postives = 120/325 (36.92%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQ---SPNPKTSTRTKGLLMNFKSQKI---LEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYS----YYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSN------PEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PINC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
CL CFC G T C+S+ + + +P + + L+ +S +I V ++ + S+ +G+ + + G G + SY Y L N + G + D + ND + V Y + + F + S W++ + A R ++ L+D+ +L+R Y ++ +++ T S N A +E+C CP Y GLSC+ C GY + + C P C C + G C+ C G C +C PGF DC C C
Sbjct: 1183 CLPCFCMGVTQQCTSSTYHRKLISTPFLPGNFQNFALVNRQRSSRITSGFTVEMSRHGPQLTHSSFDQLGQE--------SYYWQLPEGYQGDKVASYGGKLRYTLTYNAGIRGSALPD-------------ADVQITGNDITLVAYQHDLRPRESKSFEIVFRESSWKRPDG-------QPATREHLMMALADLDEILVRASYSTDMLSSSITGVSME---TAGPSYTNLPQALEVEECRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEPCYCNGHSESCHPETGACSNCLHNTVGESCDQCAPGFYGDASAGTPEDCQPCAC 1476 HSP 4 Score: 56.9954 bits (136), Expect = 8.365e-7 Identity = 62/284 (21.83%), Postives = 105/284 (36.97%), Query Frame = -3
Query: 5385 KCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTEN--------IILVKRNP-------NQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6176
+C+CK N +G +C C P F + CL CFC TQ+C S +R + IS + N N LVNR + + + + P +Q+G +L D + YGGK+ +T YN + P +++ G T++++ + ES E F + + RE + AL + + + + + +++E A +L + VE C CP G G+ C+ C
Sbjct: 1153 QCECKPNVEGASCGTCRPHHFYLSTENPDGCLPCFCMGVTQQCTSSTYHR-------------------KLISTPFLPGNFQNFALVNRQRSSRITSGFTVEMSRHGPQLTHSSFDQLGQESYYWQLPEGYQGDKVAS----YGGKLRYTLTYNAG-----IRGSALPDADVQIT--GNDITLVAYQHDLRPRESKSFEIVFRESSWKRPDGQPATREHLMMALADLDEILVRASYSTDMLSS-SITGVSMETAGPSYTNLPQALEVEECRCPPGYQGLSCQDC 1405
BLAST of SMED30010499 vs. Ensembl Xenopus
Match: LAMA5 (laminin subunit alpha 5 [Source:NCBI gene;Acc:100145762]) HSP 1 Score: 71.2478 bits (173), Expect = 2.009e-14 Identity = 78/316 (24.68%), Postives = 134/316 (42.41%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKI-LEVSYAKSIYDVNSKSYKFVGKMYQDFVVD-PNVFLTSDFGMDGSWLESYSYYLK--INIRLFGESKDDIP---GPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PINCV----EC-DKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
C +CFC G TD C +A + + S +L+ Q++ + V + + D N + V +YQ+F P +L G + SY +L+ ++ + + +IP P +IL+ + T + E ++ ++ + E F+ R +++ VL++++ LLIR + + +D I + S+G + AP +E C CP Y G SC++C GY ++ + + KC P NC +C G C +C G +C CK GFV S C C C
Sbjct: 1559 CTRCFCFGATDRCHTASKH----RAEFSDMNGWVLVGGDRQEVEISVRPEEGLVDANLED---VPDVYQEFYWHAPRSYL-------GDRVSSYGGFLRYELHSKAIRGDQPNIPVERRPDIILKGNQMSIAHLQTR-----YPMSGEHYHGRIHLV----EGNFVHVQTYNPVSREELMMVLANLEQLLIRALNSQSSSSVSLRRVFLD--IGHESSVG--VRAPEVELCMCPANYRGDSCQECAPGYYRDTKGLFLGKCVPCNCGGHSDQCLPGTGACVKCQHNTEGDRCERCKDGFVSNGTVDGSLQCVSCPC 1847 HSP 2 Score: 31.187 bits (69), Expect = 2.009e-14 Identity = 26/85 (30.59%), Postives = 40/85 (47.06%), Query Frame = -3
Query: 6060 CVINAYRNLCQLLMNETAKKRDLSENKNCNSLGTE-NIVDGKCK-CKENYKGPNCSECSPGFFS---FQKKCLKCFCSN-KTQEC 6296
C Y N +++ + K D + N + N L ++ + + G C C N G +C C+PGF+ K C +C CS TQ C
Sbjct: 1883 CAPGFYGN--PMVIGSSCKPCDCNGNTDSNMLFSDCDPLFGTCSSCMFNTAGTHCEVCAPGFYGDAVRAKNCTRCDCSPCGTQSC 1965
BLAST of SMED30010499 vs. Ensembl Xenopus
Match: rabggtb (Rab geranylgeranyltransferase subunit beta [Source:Xenbase;Acc:XB-GENE-953600]) HSP 1 Score: 78.5666 bits (192), Expect = 2.561e-13 Identity = 48/132 (36.36%), Postives = 74/132 (56.06%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNCRHP 8040
CS +Q C ++ CI + C+G DC D DE + + CA C P +F+C + +PP ++C+ W CDG+ DC + YDE NC + +C S FAC + + + RS + CD +C +G DE+ C +P
Sbjct: 2892 CSSDQFRCDDARCIPASWICDGDNDCGDMSDEDQRHNCANRSCAPTEFTC--INNRPPERRCIPQSWVCDGDADCSDAYDEHQNCT---RRSCSSSEFACANGLCV--RSNFRCDRRNDCGDGSDERGCIYP 3016 HSP 2 Score: 73.559 bits (179), Expect = 8.497e-12 Identity = 42/123 (34.15%), Postives = 62/123 (50.41%), Query Frame = -2
Query: 7672 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKN 8040
C Q C + CID + C+G KDC DG DE + C ++F C + +C++ ++CDG DC + DELNC C + F C + IP + CD +P+C++G DE N
Sbjct: 1142 CEPGQFQCPDHRCIDPSYVCDGDKDCVDGSDE-MGCTYNCSYSEFKCAS------GDQCISTGYQCDGVFDCNDHSDELNCPTRPAGMCHQNEFQCQSDGACIPSNWECDGHPDCIDGSDEHN 1257 HSP 3 Score: 67.3958 bits (163), Expect = 5.241e-10 Identity = 42/127 (33.07%), Postives = 57/127 (44.88%), Query Frame = -2
Query: 7639 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8019
C N C+ C+G+ DC D DE A C +C + + +C+ WRCD NDC + DE NC +C + F C +N IPR CD + +C +G DE NC P Q
Sbjct: 1026 CANGKCVPVYDRCDGVDDCHDNSDE-----ANCGTRNNTCSSRAFTCGNGQCIPLNWRCDSHNDCVDRSDEQNCPTQGPRSCSSTSFTCQNN-RCIPRIWLCDTDNDCGDGSDELNCNFTSTCEPGQ 1146 HSP 4 Score: 67.0106 bits (162), Expect = 6.910e-10 Identity = 45/126 (35.71%), Postives = 61/126 (48.41%), Query Frame = -2
Query: 7660 CR-NSICIDENKFCNGIKDCEDGFDEEINTCAQCEP-NQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR----HP 8019
CR N C+ CNG DC D DE+ C+P F C + +C+ W+CDG+NDC +G DE NC + C S F C DN+ IP CD + +C + DE++C HP
Sbjct: 3672 CRTNYRCVPMWSVCNGYDDCRDNSDEQGCEQRTCDPRGDFRCD-------NHRCIPLRWKCDGDNDCNDGSDERNCSPRE---CTESEFRC-DNLRCIPGRWICDHDNDCEDNSDERDCEIRTCHP 3786 HSP 5 Score: 65.0846 bits (157), Expect = 2.800e-9 Identity = 45/126 (35.71%), Postives = 61/126 (48.41%), Query Frame = -2
Query: 7663 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH 8040
CS C N CI N C+ DC D DE+ C P SC + + +C+ W CD +NDCG+G DELNC +TC F C D+ + P S CD + +C++G DE C +
Sbjct: 1060 CSSRAFTCGNGQCIPLNWRCDSHNDCVDRSDEQ--NCPTQGPR--SCSSTSFTCQNNRCIPRIWLCDTDNDCGDGSDELNCNF--TSTCEPGQFQCPDHRCIDP-SYVCDGDKDCVDGSDEMGCTY 1178 HSP 6 Score: 64.6994 bits (156), Expect = 4.364e-9 Identity = 46/139 (33.09%), Postives = 65/139 (46.76%), Query Frame = -2
Query: 7636 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDE---EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQE 8040
CS+++ C + CI C+G+ DC D DE C N+F C + G C+ + W CDG DC +G DE N + +C S F CD N I RS CD + +C + DEK+C P P+ +
Sbjct: 1180 CSYSEFKCASGDQCISTGYQCDGVFDCNDHSDELNCPTRPAGMCHQNEFQCQSDGA------CIPSNWECDGHPDCIDGSDEHNTCPV--RSCPPSMFRCD-NGNCIYRSWICDGDNDCRDMSDEKDCPTPPFRCPSWQ 1309 HSP 7 Score: 63.929 bits (154), Expect = 6.804e-9 Identity = 41/124 (33.06%), Postives = 60/124 (48.39%), Query Frame = -2
Query: 7678 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8043
+C Q C + C CN DC DG DE+ CA QC+ +Q+ C +++C+ W+CD E+DCG+ DE + TC F C +N IP+S CD + +C + DE
Sbjct: 3538 YCRVGQFQCNDGNCTSSYFMCNSYPDCPDGSDEDQILCANHQCDTHQWQC-------ANKRCIPESWQCDQEDDCGDNSDE-DSAHCSTRTCNPGQFRC-NNGRCIPQSWKCDVDDDCGDHSDE 3652 HSP 8 Score: 62.7734 bits (151), Expect = 1.398e-8 Identity = 47/144 (32.64%), Postives = 64/144 (44.44%), Query Frame = -2
Query: 7678 VYECVHNNLLIKATLIVKDFCSFNQRACR-NSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8100
V++C ++ + C N+ C+ + CI N C+G DC DG DE NTC C P+ F C + C+ W CDG+NDC + DE +C C + C N I S CD P+C NG DE
Sbjct: 1203 VFDCNDHSDELNCPTRPAGMCHQNEFQCQSDGACIPSNWECDGHPDCIDGSDEH-NTCPVRSCPPSMFRCD-------NGNCIYRSWICDGDNDCRDMSDEKDC-PTPPFRCPSWQWQCPGNTICINVSKVCDNTPDCPNGADE 1337 HSP 9 Score: 62.003 bits (149), Expect = 2.493e-8 Identity = 45/127 (35.43%), Postives = 56/127 (44.09%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
CS Q C + CI C+ +KDC DG DE C + SC + C RCDG DC + DE NC + CL S F C IPR CD + +C + DEKNC +P
Sbjct: 104 CSAQQFTCSSGQCIPRAYRCDRMKDCTDGADER--NCLYPVCTELSCGS-------GACYNRSQRCDGIVDCRDSSDESNCTQ----RCLSSQFQCPSG-ECIPRDFLCDHDDDCGDESDEKNCTYP 216 HSP 10 Score: 58.5362 bits (140), Expect = 2.538e-7 Identity = 46/130 (35.38%), Postives = 60/130 (46.15%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCI--KLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNG--EDEKNC 8040
C + C N CI E C+ DC DG DE CA C F+C + +CV ++RCD NDCG+ DEL C+ D NT F C +N I R+ C+ NC + DE+NC
Sbjct: 2686 CENGKFTCLNGRCIPERHKCDNDNDCRDGSDELERVCAFHTCPSTSFTCG-------NGRCVPYHYRCDHYNDCGDNSDELGCLFRTCDPNT----EFTC-NNGRCISRAYVCNGVNNCFDNGTSDERNC 2803 HSP 11 Score: 58.5362 bits (140), Expect = 2.854e-7 Identity = 39/136 (28.68%), Postives = 61/136 (44.85%), Query Frame = -2
Query: 7663 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDD--------NVTLIPRSAWCDENPNCLNGEDEKNCRH 8040
C +Q C N CI E+ C+ DC D DE+ C+ C P QF C+ + +C+ W+CD ++DCG+ DE C+G + CD+ N +P + C+ +C + DE+ C
Sbjct: 3580 CDTHQWQCANKRCIPESWQCDQEDDCGDNSDEDSAHCSTRTCNPGQFRCN-------NGRCIPQSWKCDVDDDCGDHSDEP------FQECMGPAYRCDNHTDFDCRTNYRCVPMWSVCNGYDDCRDNSDEQGCEQ 3702 HSP 12 Score: 56.6102 bits (135), Expect = 9.535e-7 Identity = 42/129 (32.56%), Postives = 55/129 (42.64%), Query Frame = -2
Query: 7663 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH 8040
C CR++ ICI C+G DC D DE C C ++F C + +C+ +W CD DC +G DE TC F CDD IP S CD + +C + DE RH
Sbjct: 2808 CQPGYTKCRSTNICIPRTYLCDGDNDCGDMSDESPTHCVTLTCTESEFRCSS-------GRCIPGHWYCDQGVDCSDGSDEPPTCVAHVRTCSSDQFRCDD-ARCIPASWICDGDNDCGDMSDEDQ-RH 2927 HSP 13 Score: 55.8398 bits (133), Expect = 1.911e-6 Identity = 40/130 (30.77%), Postives = 58/130 (44.62%), Query Frame = -2
Query: 7669 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPN-QFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8043
+C + C+N +CI C+G DC DG DEE+ C CEP +F C + +CV + C+G +DC +G DE C C + C N +P CD+ +C + DE C
Sbjct: 3827 YCPASMFECKNHVCIQPYWRCDGDNDCGDGSDEELQHCLDIPCEPPFRFRCG-------NNRCVYRHEICNGVDDCSDGSDETEEQCEPPTPRPCTDEEYKC-GNHFCVPLHYVCDDYDDCGDHSDEAGC 3948 HSP 14 Score: 55.8398 bits (133), Expect = 2.078e-6 Identity = 42/125 (33.60%), Postives = 61/125 (48.80%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL-NCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS ++ AC N +C+ N C+ DC DG DE C Q G+ C++ + CDG+NDCG+ DEL + + TC +F C DN I + + C+ N +C + DEK C
Sbjct: 2979 CSSSEFACANGLCVRSNFRCDRRNDCGDGSDER--GCIYPTCQQQQFTCQNGR-----CISKAFVCDGDNDCGDESDELEHTCTTSEATCNPHYFKC-DNWICIAQGSVCNGNDDCGDNSDEKAC 3095 HSP 15 Score: 55.8398 bits (133), Expect = 2.113e-6 Identity = 41/125 (32.80%), Postives = 57/125 (45.60%), Query Frame = -2
Query: 7675 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNT--CLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEK 8040
C+ ++ C N CI C+ DCED DE C P F C++ C+A +RCDG DC + DE C N C S F C ++V + P W CD + +C +G DE+
Sbjct: 3745 CTESEFRCDNLRCIPGRWICDHDNDCEDNSDERDCEIRTCHPGYFQCNS-------GHCIAERFRCDGTADCLDVSDEAACPTRYPNGTYCPASMFECKNHVCIQP--YWRCDGDNDCGDGSDEE 3860 HSP 16 Score: 54.299 bits (129), Expect = 5.136e-6 Identity = 42/122 (34.43%), Postives = 55/122 (45.08%), Query Frame = -2
Query: 7660 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGED--EKNCRHP 8019
C + CI E C+G DC D DE C PN C + + C+ YWRCDG+NDCG+G DE LD F C +N + R C+ +C +G D E+ C P
Sbjct: 3791 CNSGHCIAERFRCDGTADCLDVSDEA--ACPTRYPNGTYCPASMFECKNHVCIQPYWRCDGDNDCGDGSDEELQHCLDIPCEPPFRFRCGNN-RCVYRHEICNGVDDCSDGSDETEEQCEPP 3909
BLAST of SMED30010499 vs. Ensembl Mouse
Match: Hspg2 (perlecan (heparan sulfate proteoglycan 2) [Source:MGI Symbol;Acc:MGI:96257]) HSP 1 Score: 204.142 bits (518), Expect = 1.976e-59 Identity = 170/619 (27.46%), Postives = 260/619 (42.00%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ---CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMF---NNAAMFVSMCLKCFCSGQTDDCSSAMGY-------YQSPNPKTSTRTKGLLMNFKSQ--------KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C + +C + CI + C+G +DC DG DE CA CEPN+F+C + C WRCDG+ DC + DE NC +K C +HF C IP S CDE +C + DE C P+VV P Q++ +++ C VPT INWR NWG +P T+ G L I ++ G YTCEA++++ + V + C F ++A+ CL CFC G T+ C S++ + + PN KG+ + SQ L++ A + + S +F+ ++ F P FL G+ ++SY +L+ +R L + + P VIL G L+ S G NQ ++ L E E+W +S R ++L L+ ++ +L++ Y + + +D +T+ G A +E+C CP Y+GLSCE C A + + + C NC CD G+C C GP+C +CKPGF + ++ C C C + S+ C D GQ C C+ C
Sbjct: 285 CGPQEASCHSGHCIPRDYLCDGQEDCRDGSDEL--GCASPPPCEPNEFACE-------NGHCALKLWRCDGDFDCEDRTDEANCSVKQPGEVCGPTHFQCVSTNRCIPASFHCDEESDCPDRSDEFGCMPPQVVTPPQQSIQASRGQ-TVTFTCVATGVPTPIINWRLNWGHIPAHPRVTMTSEGG-----RGTLIIRDVKEADQGAYTCEAMNSRGMVFGIPDGVLELVPQRGPCPDGHFYLEDSAS-----CLPCFCFGVTNVCQSSLRFRDQIRLSFDQPND-----FKGVNVTMPSQPGVPPLSSTQLQIDPALQEFQLVDLSRRFL--VHDAFWALPKQFL-------GNKVDSYGGFLRYKVRYELARGMLEPVQKPDVILVGA-GYRLH------SRGHTPTHPGTLNQRQVQLSE-EHWVHESG--RPVQRAEMLQALASLEAVLLQTVYNTKMASVGLSDIVMDTTVTHTTIHGR---AHSVEECRCPIGYSGLSCESCDAHFTRVPGGPYLGTCSGCNCNGHASSCDPVYGHCLNCQHNTEGPQCDKCKPGFFGDATKATATACRPCPCPYIDASRRFSDTCFLDTDGQA--TCDACAPGYTGRRCESC 854 HSP 2 Score: 48.9062 bits (115), Expect = 1.976e-59 Identity = 23/62 (37.10%), Postives = 31/62 (50.00%), Query Frame = -3
Query: 6039 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6212
C+ G+ C+CK N G C+ECS G F K+ CLKCFC +++C S R
Sbjct: 879 CDERGSLGTSGETCRCKNNVVGRLCNECSDGSFHLSKQNPDGCLKCFCMGVSRQCSSSSWSR 940 HSP 3 Score: 72.4034 bits (176), Expect = 3.225e-13 Identity = 84/365 (23.01%), Postives = 131/365 (35.89%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDC-------SSAMGYYQSPN-------PKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINI--RLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
CLKCFC G + C + +G + P+ T T ++G+ S E+S++ S +++ S+ Y F + G + SY L+ + R S P V+LQ N + + D S P F + W++ + A R +L L+ I LLI+ Y ++D + P++ G + A +E+C CP Y G SC+ C GY + + C NC C+ + G C C G C +C+PG+ DC C CY ++ C D G P CD C
Sbjct: 921 CLKCFCMGVSRQCSSSSWSRAQVLGASEQPSQFSLSNAAGTHTTSEGV----SSPAPGELSFS-SFHNLLSEPY----------------FWSLPASFRGDKVTSYGGELRFTVTQRPRPSSAPLHRQPLVVLQGNNIVLEHHASRDPSP---GQPSNFIVPFQEQAWQRPD-------GQPATREHLLMALAGIDALLIQASYTQQPAESRVSGISMD--VAVPENTGQD-SAREVEQCTCPPGYRGPSCQDCDTGYTRVPSGLYLGTCERCNCHGHSETCEPETGACQSCQHHTEGASCEQCQPGYYGDAQRGTPQDCQPCPCYGAPAAGQAAHTCFLDTDGH--------------PTCDSC 1237 HSP 4 Score: 25.409 bits (54), Expect = 3.225e-13 Identity = 6/18 (33.33%), Postives = 11/18 (61.11%), Query Frame = -3
Query: 6114 CKENYKGPNCSECSPGFF 6167
C + G +C C+PG++
Sbjct: 1237 CSPGHSGRHCERCAPGYY 1254 HSP 5 Score: 65.855 bits (159), Expect = 1.215e-9 Identity = 49/191 (25.65%), Postives = 75/191 (39.27%), Query Frame = -2
Query: 6283 AMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR---IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLS---SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6813
A R +L L+D+ LL+R + S +++ P S PR +E+C CP Y GLSC+ C GY + + +C + EC+ + G C+RC +G C C G+ YG+ + DC C C + S C + G G C C++C
Sbjct: 1476 ATREHLLMALADLDELLVRATFSSVPRAASISAVSLEVAQPGPSS------GPRALEVEECRCPPGYVGLSCQDCAPGYTRTGSGLYLGQCEL--CECNGHSDLCHPETGACSRCQHNTAGEFCELCATGY---YGDATAGTPEDCQPCACPLTNPENMFSRTC--ESLGAGGYRCTACEPGYTGQYCEQC 1653
BLAST of SMED30010499 vs. Ensembl Mouse
Match: Hspg2 (perlecan (heparan sulfate proteoglycan 2) [Source:MGI Symbol;Acc:MGI:96257]) HSP 1 Score: 204.142 bits (518), Expect = 1.976e-59 Identity = 170/619 (27.46%), Postives = 260/619 (42.00%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ---CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMF---NNAAMFVSMCLKCFCSGQTDDCSSAMGY-------YQSPNPKTSTRTKGLLMNFKSQ--------KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C + +C + CI + C+G +DC DG DE CA CEPN+F+C + C WRCDG+ DC + DE NC +K C +HF C IP S CDE +C + DE C P+VV P Q++ +++ C VPT INWR NWG +P T+ G L I ++ G YTCEA++++ + V + C F ++A+ CL CFC G T+ C S++ + + PN KG+ + SQ L++ A + + S +F+ ++ F P FL G+ ++SY +L+ +R L + + P VIL G L+ S G NQ ++ L E E+W +S R ++L L+ ++ +L++ Y + + +D +T+ G A +E+C CP Y+GLSCE C A + + + C NC CD G+C C GP+C +CKPGF + ++ C C C + S+ C D GQ C C+ C
Sbjct: 285 CGPQEASCHSGHCIPRDYLCDGQEDCRDGSDEL--GCASPPPCEPNEFACE-------NGHCALKLWRCDGDFDCEDRTDEANCSVKQPGEVCGPTHFQCVSTNRCIPASFHCDEESDCPDRSDEFGCMPPQVVTPPQQSIQASRGQ-TVTFTCVATGVPTPIINWRLNWGHIPAHPRVTMTSEGG-----RGTLIIRDVKEADQGAYTCEAMNSRGMVFGIPDGVLELVPQRGPCPDGHFYLEDSAS-----CLPCFCFGVTNVCQSSLRFRDQIRLSFDQPND-----FKGVNVTMPSQPGVPPLSSTQLQIDPALQEFQLVDLSRRFL--VHDAFWALPKQFL-------GNKVDSYGGFLRYKVRYELARGMLEPVQKPDVILVGA-GYRLH------SRGHTPTHPGTLNQRQVQLSE-EHWVHESG--RPVQRAEMLQALASLEAVLLQTVYNTKMASVGLSDIVMDTTVTHTTIHGR---AHSVEECRCPIGYSGLSCESCDAHFTRVPGGPYLGTCSGCNCNGHASSCDPVYGHCLNCQHNTEGPQCDKCKPGFFGDATKATATACRPCPCPYIDASRRFSDTCFLDTDGQA--TCDACAPGYTGRRCESC 854 HSP 2 Score: 48.9062 bits (115), Expect = 1.976e-59 Identity = 23/62 (37.10%), Postives = 31/62 (50.00%), Query Frame = -3
Query: 6039 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6212
C+ G+ C+CK N G C+ECS G F K+ CLKCFC +++C S R
Sbjct: 879 CDERGSLGTSGETCRCKNNVVGRLCNECSDGSFHLSKQNPDGCLKCFCMGVSRQCSSSSWSR 940 HSP 3 Score: 72.4034 bits (176), Expect = 3.226e-13 Identity = 84/365 (23.01%), Postives = 131/365 (35.89%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDC-------SSAMGYYQSPN-------PKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINI--RLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
CLKCFC G + C + +G + P+ T T ++G+ S E+S++ S +++ S+ Y F + G + SY L+ + R S P V+LQ N + + D S P F + W++ + A R +L L+ I LLI+ Y ++D + P++ G + A +E+C CP Y G SC+ C GY + + C NC C+ + G C C G C +C+PG+ DC C CY ++ C D G P CD C
Sbjct: 921 CLKCFCMGVSRQCSSSSWSRAQVLGASEQPSQFSLSNAAGTHTTSEGV----SSPAPGELSFS-SFHNLLSEPY----------------FWSLPASFRGDKVTSYGGELRFTVTQRPRPSSAPLHRQPLVVLQGNNIVLEHHASRDPSP---GQPSNFIVPFQEQAWQRPD-------GQPATREHLLMALAGIDALLIQASYTQQPAESRVSGISMD--VAVPENTGQD-SAREVEQCTCPPGYRGPSCQDCDTGYTRVPSGLYLGTCERCNCHGHSETCEPETGACQSCQHHTEGASCEQCQPGYYGDAQRGTPQDCQPCPCYGAPAAGQAAHTCFLDTDGH--------------PTCDSC 1237 HSP 4 Score: 25.409 bits (54), Expect = 3.226e-13 Identity = 6/18 (33.33%), Postives = 11/18 (61.11%), Query Frame = -3
Query: 6114 CKENYKGPNCSECSPGFF 6167
C + G +C C+PG++
Sbjct: 1237 CSPGHSGRHCERCAPGYY 1254 HSP 5 Score: 65.855 bits (159), Expect = 1.215e-9 Identity = 49/191 (25.65%), Postives = 75/191 (39.27%), Query Frame = -2
Query: 6283 AMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR---IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLS---SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6813
A R +L L+D+ LL+R + S +++ P S PR +E+C CP Y GLSC+ C GY + + +C + EC+ + G C+RC +G C C G+ YG+ + DC C C + S C + G G C C++C
Sbjct: 1476 ATREHLLMALADLDELLVRATFSSVPRAASISAVSLEVAQPGPSS------GPRALEVEECRCPPGYVGLSCQDCAPGYTRTGSGLYLGQCEL--CECNGHSDLCHPETGACSRCQHNTAGEFCELCATGY---YGDATAGTPEDCQPCACPLTNPENMFSRTC--ESLGAGGYRCTACEPGYTGQYCEQC 1653
BLAST of SMED30010499 vs. Ensembl Mouse
Match: Ldlr (low density lipoprotein receptor [Source:MGI Symbol;Acc:MGI:96765]) HSP 1 Score: 86.2705 bits (212), Expect = 5.760e-16 Identity = 51/130 (39.23%), Postives = 69/130 (53.08%), Query Frame = -2
Query: 7666 KDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR 8049
+D CS N+ CR+ CI C+G +C DG DE TC C+ NQFSC GG+ +C+ + WRCDG+ DC N DE C TC F C D + P+ CD + +CL+G DE +C+
Sbjct: 24 EDSCSRNEFQCRDGKCIASKWVCDGSPECPDGSDESPETCMSVTCQSNQFSC---GGRV--SRCIPDSWRCDGQVDCENDSDEQGCP---PKTCSQDDFRCQDGKCISPQFV-CDGDRDCLDGSDEAHCQ 144 HSP 2 Score: 59.3066 bits (142), Expect = 1.002e-7 Identity = 42/132 (31.82%), Postives = 59/132 (44.70%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NCIKLD------KNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS + C++ CI C+G +DC DG DE C P F C++ C+ + W CDG+ DC +G DE NC D + C F C + I RS CD +C + DE++C
Sbjct: 109 CSQDDFRCQDGKCISPQFVCDGDRDCLDGSDEAHCQATTCGPAHFRCNS-------SICIPSLWACDGDVDCVDGSDEWPQNCQGRDTASKGVSSPCSSLEFHCGSS-ECIHRSWVCDGEADCKDKSDEEHC 232 HSP 3 Score: 53.5286 bits (127), Expect = 5.133e-6 Identity = 45/150 (30.00%), Postives = 68/150 (45.33%), Query Frame = -2
Query: 7624 ATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSH-FACDDNVTLIPRSAWCDENPNCLNGEDE--KNCRHPEVVIPNQETTYV 8064
A+ V CS + C +S CI + C+G DC+D DEE A C P++F C C+ +CD E+DC + DEL C+ + + C G + F C I CD +C + DE K C+ E + N +++
Sbjct: 190 ASKGVSSPCSSLEFHCGSSECIHRSWVCDGEADCKDKSDEEHCAVATCRPDEFQC-------ADGSCIHGSRQCDREHDCKDMSDELGCVNVTQ--CDGPNKFKCHSG-ECISLDKVCDSARDCQDWSDEPIKECKTNECLDNNGGCSHI 329
BLAST of SMED30010499 vs. Ensembl Mouse
Match: Ldlr (low density lipoprotein receptor [Source:MGI Symbol;Acc:MGI:96765]) HSP 1 Score: 86.2705 bits (212), Expect = 5.954e-16 Identity = 51/130 (39.23%), Postives = 69/130 (53.08%), Query Frame = -2
Query: 7666 KDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR 8049
+D CS N+ CR+ CI C+G +C DG DE TC C+ NQFSC GG+ +C+ + WRCDG+ DC N DE C TC F C D + P+ CD + +CL+G DE +C+
Sbjct: 24 EDSCSRNEFQCRDGKCIASKWVCDGSPECPDGSDESPETCMSVTCQSNQFSC---GGRV--SRCIPDSWRCDGQVDCENDSDEQGCP---PKTCSQDDFRCQDGKCISPQFV-CDGDRDCLDGSDEAHCQ 144 HSP 2 Score: 59.3066 bits (142), Expect = 9.685e-8 Identity = 42/132 (31.82%), Postives = 59/132 (44.70%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NCIKLD------KNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS + C++ CI C+G +DC DG DE C P F C++ C+ + W CDG+ DC +G DE NC D + C F C + I RS CD +C + DE++C
Sbjct: 109 CSQDDFRCQDGKCISPQFVCDGDRDCLDGSDEAHCQATTCGPAHFRCNS-------SICIPSLWACDGDVDCVDGSDEWPQNCQGRDTASKGVSSPCSSLEFHCGSS-ECIHRSWVCDGEADCKDKSDEEHC 232 HSP 3 Score: 53.5286 bits (127), Expect = 5.199e-6 Identity = 45/150 (30.00%), Postives = 68/150 (45.33%), Query Frame = -2
Query: 7624 ATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSH-FACDDNVTLIPRSAWCDENPNCLNGEDE--KNCRHPEVVIPNQETTYV 8064
A+ V CS + C +S CI + C+G DC+D DEE A C P++F C C+ +CD E+DC + DEL C+ + + C G + F C I CD +C + DE K C+ E + N +++
Sbjct: 190 ASKGVSSPCSSLEFHCGSSECIHRSWVCDGEADCKDKSDEEHCAVATCRPDEFQC-------ADGSCIHGSRQCDREHDCKDMSDELGCVNVTQ--CDGPNKFKCHSG-ECISLDKVCDSARDCQDWSDEPIKECKTNECLDNNGGCSHI 329
BLAST of SMED30010499 vs. Ensembl Mouse
Match: Lama1 (laminin, alpha 1 [Source:MGI Symbol;Acc:MGI:99892]) HSP 1 Score: 78.9518 bits (193), Expect = 9.045e-16 Identity = 91/368 (24.73%), Postives = 155/368 (42.12%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS-AMGYYQSPNPKTSTRTKGLLMN-FKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNP-EGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ-------NQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C+ +N C +CFC G + C S Q N T + N +SQ+ + + + I N+ + + Y + P +L + G +L+ Y + +I + D + +I++ NG ++ + +E + P E ++N +++ EN F D R ++ VL+++ +LLIR Y S + + +V I +P+++ + A +E C CP YTG SCE CL GY + D C +C + ECD +G C+ CT +G C +C PGF DC C C +++ S C LT +VC + C++C
Sbjct: 492 CKPGFYNLKERNPEGCSECFCFGVSGVCDSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDV-LGGHRQISINNTAVMQRLTSTY--YWAAPEAYLGNKLTAFGGFLK---YTVSYDIPVETVDSDLMSHADIIIKG-NGLTI----STRAEGLSLQPYEEYFNVVRLV---PEN-FRDFDTRREIDRDQLMTVLANVTHLLIRANYNSAKMALYRLD-SVSLDIASPNAIDLAV-AADVEHCECPQGYTGTSCEACLPGYYRVDGILFGGICQPC--ECHGHASECDIHGICSVCTHNTTGDHCEQCLPGFYGTPSRGTPGDCQPCACPLSIDSNNFSPTC--HLTDGEEVVCDQCAPGYSGSWCERC 838 HSP 2 Score: 27.335 bits (59), Expect = 9.045e-16 Identity = 19/55 (34.55%), Postives = 25/55 (45.45%), Query Frame = -3
Query: 6081 DLSENKNCNSLGTENIVDGKC-KCKENYKGPNCSECSPGFFS---FQKKCLKCFC 6233
D E +C+S V G+C KC N G +C C+ GF+ K C C C
Sbjct: 863 DPLEAGHCDS------VTGECLKCLWNTDGAHCERCADGFYGDAVTAKNCRACDC 911 HSP 3 Score: 69.3218 bits (168), Expect = 1.020e-10 Identity = 72/294 (24.49%), Postives = 126/294 (42.86%), Query Frame = -3
Query: 5385 NCNSLGT--ENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNR-----QISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLF-----MEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKW-MNDLGKIAPVEMCLCPKGNPGIKCEAC 6215
+C ++G+ E+ C CK+N +G NC C PGF++ +++ C +CFC + C +S + S +T S + D++ +N+ + G + + NN ++ R + Y+ KLT +GG + +T Y D+ + V SD+ +I K G + + + E F + +NF + +E DR+ + L HL I +++ + RL ++++ A DL A VE C CP+G G CEAC
Sbjct: 462 DCRTVGSLNEDPCIEPCLCKKNVEGKNCDRCKPGFYNLKERNPEGCSECFCFGVSGVC-DSLTWSISQVTNMSGWLVTDLMSTNKIRSQQDVLGGHRQISINNTAVMQRLTSTYYWAAPE-----AYLGNKLT-------------AFGGFLKYTVSY--DIPVETVDSDLMSHADIIIKGNGLTISTRAEGLSLQPYEEYFNVVRLVPENFRDFDTRRE----IDRDQLMTVLANVTHLLIRANYNSAKMALYRLDSVSLDIASPNAIDLAVAADVEHCECPQGYTGTSCEAC 730 HSP 4 Score: 59.6918 bits (143), Expect = 9.718e-8 Identity = 63/276 (22.83%), Postives = 116/276 (42.03%), Query Frame = -3
Query: 5385 GKCKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLF---MEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDL---GKIA-PVEMCLCPKGNPGIKCEAC 6179
G C CKEN GP CS+C G F+ + + C CFC +Q C E + Y + +TL S D L V+S + G E + + ++ + I +L D + L YGGK+ ++ + + L S+ P + I+ + + + + + M++ F K+ K + + F+ L ++ I + G ++ R+ ++++E + +L G+ A +E+C+CP G G C+ C
Sbjct: 1123 GTCSCKENAVGPQCSKCQAGTFALRGDNPQGCSPCFCFGLSQLCSELEGYVRTLITLAS-DQPLLHVVSQSNLKGTIEG------VHFQPPDTLLDAEAVRQHIYAEPFYWRLPKQFQGDQL----LAYGGKLQYSVAFYSTLGTGT--SNYEPQVLIKGGRARKHVIYMDAPAPENGVRQDYEVRMKEEFWKYFNSVSEKHVTHSD-FMSVLSNIDYILIKASYGQG-LQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDC 1383 HSP 5 Score: 58.9214 bits (141), Expect = 1.649e-7 Identity = 76/326 (23.31%), Postives = 123/326 (37.73%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVF-LTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSN---PEGFYNQLKISLWEKENW-FIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP----INCVECDKN----------GNCNRCTGQRSGPKCLECKPGFVRLYGE--KLSSDCTECNC 7311
C CFC G + CS GY ++ S + L++ SQ L+ + + ++ + + Q +P + L F G L +Y L+ ++ + P+V+++ + VIY + PE Q ++E W + +S E D ++VLS+I Y+LI+ Y N +++ + A +E C CP G SC+ C GY + + + P CV C+ N G C C SG C C G+ YG+ L DCT C C
Sbjct: 1154 CSPCFCFGLSQLCSELEGYVRTLITLASDQP---LLHVVSQSNLKGTI-EGVHFQPPDTLLDAEAVRQHIYAEPFYWRLPKQF--QGDQLLAYGGKLQYSVAFYSTLGTGTSNYEPQVLIKGGRARK---------HVIYMDAPAPENGVRQDYEVRMKEEFWKYFNSVSEKHVTHSDFMSVLSNIDYILIKASYGQGLQQSRIANISMEVGRKAVELPAEGEAALLLELCVCPPGTAGHSCQDCAPGYYREKLPESGGRGPRPLLAPCVPCNCNNHSDVCDPETGKCLSCRDHTSGDHCELCASGY---YGKVTGLPGDCTPCTC 1461 HSP 6 Score: 39.6614 bits (91), Expect = 3.953e-7 Identity = 42/135 (31.11%), Postives = 59/135 (43.70%), Query Frame = -2
Query: 6280 NCPTPYTGLSCEKCLAGYEKNDQ---NQCVK-KC-----PINCVECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSS-DCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKC----TGRERNDPLCDKCL 6639
C Y+G CE+C GY N CV C P+ CD G C +C G C C GF YG+ +++ +C C+C+ N +S IC + TG +C C TG++ CD+CL
Sbjct: 825 QCAPGYSGSWCERCADGYYGNPTVPGGTCVPCNCSGNVDPLEAGHCDSVTGECLKCLWNTDGAHCERCADGF---YGDAVTAKNCRACDCH---ENGSLSGICHLE-TG----LCDCKPHVTGQQ-----CDQCL 943 HSP 7 Score: 36.965 bits (84), Expect = 3.953e-7 Identity = 15/42 (35.71%), Postives = 21/42 (50.00%), Query Frame = -3
Query: 6060 DGKCKCKENYKGPNCSECSPGFFSFQ-KKCLKCFCSNKTQEC 6182
+G+C C G C CS GF++FQ C C C++ C
Sbjct: 973 EGQCHCGPGVSGKQCDRCSHGFYAFQDGGCTPCDCAHTQNNC 1014
BLAST of SMED30010499 vs. UniProt/SwissProt
Match: sp|P98160|PGBM_HUMAN (Basement membrane-specific heparan sulfate proteoglycan core protein OS=Homo sapiens OX=9606 GN=HSPG2 PE=1 SV=4) HSP 1 Score: 206.453 bits (524), Expect = 6.918e-59 Identity = 173/620 (27.90%), Postives = 257/620 (41.45%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMF---NNAAMFVSMCLKCFCSGQTDDCSSAMGY-------YQSPNPKTSTRTKGLLMNFKSQ--------KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQG---AIVCKCTGRERNDPLCDKC 8040
C + ACRN CI + C+G +DCEDG DE + CEPN+F C + C WRCDG+ DC + DE NC K + C + F C IP S CDE +C + DE C P+VV P +E+ + ++ C + VPT INWR NWG +P TV G L I ++ G YTCEA++ + + V + C F ++AA CL CFC G T C S + + P+ KG+ + +Q L++ + + + S +F+ ++ F P FL G+ ++SY L+ N+R L + + P V+L + L S G NQ ++ E E+W +S R ++L VL ++ +LI+ Y + + +D +T+ S G A +E+C CP Y+GLSCE C A + + + C NC CD G+C C GP+C +CK GF + ++ C C C + S+ C D GQ A TGR C+ C
Sbjct: 285 CGPQEAACRNGHCIPRDYLCDGQEDCEDGSDELDCGPPPPCEPNEFPCG-------NGHCALKLWRCDGDFDCEDRTDEANCPTKRPEEVCGPTQFRCVSTNMCIPASFHCDEESDCPDRSDEFGCMPPQVVTPPRESIQASRGQT-VTFTCVAIGVPTPIINWRLNWGHIPSHPRVTVTSEGG-----RGTLIIRDVKESDQGAYTCEAMNARGMVFGIPDGVLELVPQRGPCPDGHFYLEHSAA-----CLPCFCFGITSVCQSTRRFRDQIRLRFDQPDD-----FKGVNVTMPAQPGTPPLSSTQLQIDPSLHEFQLVDLSRRFL--VHDSFWALPEQFL-------GNKVDSYGGSLRYNVRYELARGMLEPVQRPDVVLMGAGYRLL-------SRGHTPTQPGALNQRQVQFSE-EHWVHESG--RPVQRAELLQVLQSLEAVLIQTVYNTKMASVGLSDIAMDTTVTHATSHGR---AHSVEECRCPIGYSGLSCESCDAHFTRVPGGPYLGTCSGCNCNGHASSCDPVYGHCLNCQHNTEGPQCNKCKAGFFGDAMKATATSCRPCPCPYIDASRRFSDTCFLDTDGQATCDACAPGYTGRR-----CESC 854 HSP 2 Score: 47.3654 bits (111), Expect = 6.918e-59 Identity = 21/62 (33.87%), Postives = 29/62 (46.77%), Query Frame = -3
Query: 6039 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6212
C+ G+ C+CK N G C+EC+ G F + CLKCFC ++ C S R
Sbjct: 879 CDERGSMGTSGEACRCKNNVVGRLCNECADGSFHLSTRNPDGCLKCFCMGVSRHCTSSSWSR 940 HSP 3 Score: 76.2554 bits (186), Expect = 3.258e-20 Identity = 84/361 (23.27%), Postives = 133/361 (36.84%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDCSS-----AMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG-------PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQN----QCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
CLKCFC G + C+S A + S P + T + ++ I + + + S ++ + Y F P+ FL G + SY L+ + ++ PG P V+LQ G ++ + E P F + W++ + A R +L L+ I LLIR Y ++D + P+ G + A +E+C+CP Y G SC+ C GY + C + C + C+ + G C C GP+C +C+PG+ DC C CY ++ C D G P CD C
Sbjct: 921 CLKCFCMGVSRHCTSSSWSRAQLHGASEEPGHFSLTNAASTHTTNEGIFSPTPGELGF---SSFHRLLSGPY--FWSLPSRFL-------GDKVTSYGGELRFTV-----TQRSQPGSTPLHGQPLVVLQ---GNNIILEHHVAQEPSPGQPSTFIVPFREQAWQRPD-------GQPATREHLLMALAGIDTLLIRASYAQQPAESRVSGISMD--VAVPEETGQDP-ALEVEQCSCPPGYRGPSCQDCDTGYTRTPSGLYLGTCERCSCHGHSEACEPETGACQGCQHHTEGPRCEQCQPGYYGDAQRGTPQDCQLCPCYGDPAAGQAAHTCFLDTDGH--------------PTCDAC 1237 HSP 4 Score: 47.7506 bits (112), Expect = 3.258e-20 Identity = 23/66 (34.85%), Postives = 31/66 (46.97%), Query Frame = -3
Query: 6039 NCN---SLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6215
NC+ S+ ++ G+C+CK +G CS C P F CL CFC TQ+C S R
Sbjct: 1276 NCDPQGSVSSQCDAAGQCQCKAQVEGLTCSHCRPHHFHLSASNPDGCLPCFCMGITQQCASSAYTR 1341 HSP 5 Score: 67.781 bits (164), Expect = 2.419e-9 Identity = 49/188 (26.06%), Postives = 74/188 (39.36%), Query Frame = -2
Query: 6283 AMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLS---SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6813
A R +L L+D+ LLIR + S +++ P N A +E+C CP Y GLSC+ C GY + + C + EC+ + G C++C +G C C PG+ YG+ + DC C C + S C + G G C C++C
Sbjct: 1476 ATREHLLMALADLDELLIRATFSSVPLAASISAVSLEVAQPGPS---NRPRALEVEECRCPPGYIGLSCQDCAPGYTRTGSGLYLGHCEL--CECNGHSDLCHPETGACSQCQHNAAGEFCELCAPGY---YGDATAGTPEDCQPCACPLTNPENMFSRTC--ESLGAGGYRCTACEPGYTGQYCEQC 1653
BLAST of SMED30010499 vs. UniProt/SwissProt
Match: sp|Q05793|PGBM_MOUSE (Basement membrane-specific heparan sulfate proteoglycan core protein OS=Mus musculus OX=10090 GN=Hspg2 PE=1 SV=1) HSP 1 Score: 201.83 bits (512), Expect = 4.857e-58 Identity = 170/619 (27.46%), Postives = 260/619 (42.00%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ---CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMF---NNAAMFVSMCLKCFCSGQTDDCSSAMGY-------YQSPNPKTSTRTKGLLMNFKSQ--------KILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 8040
C + +C + CI + C+G +DC DG DE CA CEPN+F+C + C WRCDG+ DC + DE NC +K C +HF C IP S CDE +C + DE C P+VV P Q++ +++ C VPT INWR NWG +P T+ G L I ++ G YTCEA++++ + V + C F ++A+ CL CFC G T+ C S++ + + PN KG+ + SQ L++ A + + S +F+ ++ F P FL G+ ++SY +L+ +R L + + P VIL G L+ S G NQ ++ L E E+W +S R ++L L+ ++ +L++ Y + + +D +T+ G A +E+C CP Y+GLSCE C A + + + C NC CD G+C C GP+C +CKPGF + ++ C C C + S+ C D GQ C C+ C
Sbjct: 285 CGPQEASCHSGHCIPRDYLCDGQEDCRDGSDEL--GCASPPPCEPNEFACE-------NGHCALKLWRCDGDFDCEDRTDEANCSVKQPGEVCGPTHFQCVSTNRCIPASFHCDEESDCPDRSDEFGCMPPQVVTPPQQSIQASRGQ-TVTFTCVATGVPTPIINWRLNWGHIPAHPRVTMTSEGG-----RGTLIIRDVKEADQGAYTCEAMNSRGMVFGIPDGVLELVPQRGPCPDGHFYLEDSAS-----CLPCFCFGVTNVCQSSLRFRDQIRLSFDQPND-----FKGVNVTMPSQPGVPPLSSTQLQIDPALQEFQLVDLSRRFL--VHDAFWALPKQFL-------GNKVDSYGGFLRYKVRYELARGMLEPVQKPDVILVGA-GYRLH------SRGHTPTHPGTLNQRQVQLSE-EHWVHESG--RPVQRAEMLQALASLEAVLLQTVYNTKMASVGLSDIVMDTTVTHTTIHGR---AHSVEECRCPIGYSGLSCESCDAHFTRVPGGPYLGTCSGCNCNGHASSCDPVYGHCLNCQHNTEGPQCDKCKPGFFGDATKATATACRPCPCPYIDASRRFSDTCFLDTDGQA--TCDACAPGYTGRRCESC 854 HSP 2 Score: 48.9062 bits (115), Expect = 4.857e-58 Identity = 23/62 (37.10%), Postives = 31/62 (50.00%), Query Frame = -3
Query: 6039 CNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYR 6212
C+ G+ C+CK N G C+ECS G F K+ CLKCFC +++C S R
Sbjct: 879 CDERGSLGTSGETCRCKNNVVGRLCNECSDGSFHLSKQNPDGCLKCFCMGVSRQCSSSSWSR 940 HSP 3 Score: 72.0182 bits (175), Expect = 2.565e-12 Identity = 84/365 (23.01%), Postives = 131/365 (35.89%), Query Frame = -2
Query: 6283 CLKCFCSGQTDDC-------SSAMGYYQSPN-------PKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINI--RLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7311
CLKCFC G + C + +G + P+ T T ++G+ S E+S++ S +++ S+ Y F + G + SY L+ + R S P V+LQ N + + D S P F + W++ + A R +L L+ I LLI+ Y ++D + P++ G + A +E+C CP Y G SC+ C GY + + C NC C+ + G C C G C +C+PG+ DC C CY ++ C D G P CD C
Sbjct: 921 CLKCFCMGVSRQCSSSSWSRAQVLGASEQPSQFSLSNAAGTHTTSEGV----SSPAPGELSFS-SFHNLLSEPY----------------FWSLPASFRGDKVTSYGGELRFTVMQRPRPSSAPLHRQPLVVLQGNNIVLEHHASRDPSP---GQPSNFIVPFQEQAWQRPD-------GQPATREHLLMALAGIDALLIQASYTQQPAESRLSGISMD--VAVPENTGQD-SAREVEQCTCPPGYRGPSCQDCDTGYTRVPSGLYLGTCERCNCHGHSETCEPETGACQSCQHHTEGASCEQCQPGYYGDAQRGTPQDCQPCPCYGAPAAGQAAHTCFLDTDGH--------------PTCDSC 1237 HSP 4 Score: 25.409 bits (54), Expect = 2.565e-12 Identity = 6/18 (33.33%), Postives = 11/18 (61.11%), Query Frame = -3
Query: 6114 CKENYKGPNCSECSPGFF 6167
C + G +C C+PG++
Sbjct: 1237 CSPGHSGRHCERCAPGYY 1254 HSP 5 Score: 65.855 bits (159), Expect = 9.602e-9 Identity = 49/191 (25.65%), Postives = 75/191 (39.27%), Query Frame = -2
Query: 6283 AMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR---IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLS---SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6813
A R +L L+D+ LL+R + S +++ P S PR +E+C CP Y GLSC+ C GY + + +C + EC+ + G C+RC +G C C G+ YG+ + DC C C + S C + G G C C++C
Sbjct: 1476 ATREHLLMALADLDELLVRATFSSVPRAASISAVSLEGAQPGPSS------GPRALEVEECRCPPGYVGLSCQDCAPGYTRTGSGLYLGQCEL--CECNGHSDLCHPETGACSRCQHNTAGEFCELCATGY---YGDATAGTPEDCQPCACPLTNPENMFSRTC--ESLGAGGYRCTACEPGYTGQYCEQC 1653
BLAST of SMED30010499 vs. UniProt/SwissProt
Match: sp|Q06561|UNC52_CAEEL (Basement membrane proteoglycan OS=Caenorhabditis elegans OX=6239 GN=unc-52 PE=1 SV=2) HSP 1 Score: 161.384 bits (407), Expect = 1.109e-37 Identity = 184/706 (26.06%), Postives = 280/706 (39.66%), Query Frame = -2
Query: 6460 LLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGG-------ETQALLIVNTATKEMNGTVYECVH----NNLLIKATLIVKDF--------------CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRL---PQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTK-RAMAKD--------------------------------YTIFVTSKTLCE-------------KP-MFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITN--PDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PIN-----------CVECDKNGNCNRCTGQRSGPKCLECKP 8301
+ V P+E VR G F C+ R D + +RVGG ++ L +N GT Y CV N + +ATL V + C +++AC N+ C+ + C+G DC D DE CEPN+F C+ + KCV W CDG++DCG+ DELNC K + C + F C D +P S CD +C +G DE C P VV P Q T V ++ SL C+ V+VP INWR NWG + P+ L + G LTI++ P G YTCEA++ K R +A T + + CE KP F+ + CLKCFC G ++DC S+ Y + +G+ ++ ++ ++ + S + ++ + P FL G + +Y ++ I G S P V+L+ N L + V+ S+ + ++I++ E + + +A R D+L VL+D+ LIR + ++QT + + + + PD + A +E+C CP Y G SCE C GYE++ + C PI G C +C GP C C P
Sbjct: 47 ITVFPSEKEVRDGRDVSFECRA---------RTSDNSVYPTVRWARVGGPLPSSAHDSGGRLTINPVQLSDAGT-YICVSDYNGNTVEARATLSVVSYGPQEVSNGLRQAGQCMADEKACGNNECVKNDYVCDGEPDCRDRSDEANCPAISRTCEPNEFKCN-------NNKCVQKMWLCDGDDDCGDNSDELNCNAKPSSSDCKPTEFQCHDRRQCVPSSFHCDGTNDCHDGSDEVGCVQPTVVDPPQ-TNLQVPRGTTFSLTCKAVAVPEPYINWRLNWGPVCEPPRCLQTSEGGYG--------TLTIHDAQPVDQGAYTCEAINVKGRVLATPDCIVRVVDDPRPQPPQPPTAPPQRASCDTRGAVTPYPNNYGTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGDAEGVTISDIEERTIDRNTPFSFFKTGYLTFDGTTDGVAKYWRLPQRFL-------GDKVTAYGGKMEFEIEFSG-SGHHSSEPMVVLKG-NQNILVHRVRNQEHVLRSD-----SPVRITVETYETNY-EQLNGAAATREDLLMVLADLDAFLIRATHVAHQTSTSLGDVSWEIAVDRYTPDGL-----ALEVEQCVCPPGYLGTSCEDCAPGYERSGYGPYLGTCVPIQPRHQQCGPGAVAPTAPAQGQC-QCKASVIGPNCDRCAP 705 HSP 2 Score: 77.0258 bits (188), Expect = 4.153e-12 Identity = 88/342 (25.73%), Postives = 132/342 (38.60%), Query Frame = -2
Query: 6418 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSS------------AMG-----------YYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDC 7356
C F A C+ CFCSG T CS+ A G Q +P+T G + F+S E + +++Y +K P FL G + SY L+ + G D VIL+ N +L + + E Y++ E N+++I + E +D A R +L L+D+ LLI+ Y + T + +A ++ + G + A +E+C CP Y G SCE C GY + C K +C + +CDK G C C G +C CKPGFV +DC
Sbjct: 703 CAPNSFGLAPTNPQGCIPCFCSGVTQQCSASSYRRTSVSIDYARGDRDQLELTTSDSRQPYSPQTRAELSGQAIEFRS---FEEARGQTLY------WKL-----------PEKFL-------GDKVTSYGGTLEYTFKFSGNGNSDQSA-DVILRG-NDIALQY---KHREPFYADRE---NKVQIKIIETSWQRVDGQ---QATREHLLMTLADLDTLLIKSTYNDDCTDSQLLSANLEFA----EPYGQGLTAAEVEQCICPPGYVGTSCEDCAPGYSRTGGGLYLGLCEKCECNGHASQCDKEYGYCLDCQHNTEGDQCERCKPGFVGDARRGTPNDC 1002 HSP 3 Score: 60.8474 bits (146), Expect = 3.114e-7 Identity = 24/61 (39.34%), Postives = 33/61 (54.10%), Query Frame = -3
Query: 6009 GKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSAD 6179
G C+CK GPNC +C PG F +K CLKCFC + +C+ S YR + + + D
Sbjct: 398 GTCECKSQVTGPNCDQCKPGAFHLSEKSPEGCLKCFCFGVSNDCRSSGHYRTKDRLMFAGD 458
BLAST of SMED30010499 vs. UniProt/SwissProt
Match: sp|P35952|LDLR_RAT (Low-density lipoprotein receptor OS=Rattus norvegicus OX=10116 GN=Ldlr PE=1 SV=1) HSP 1 Score: 88.1965 bits (217), Expect = 1.144e-15 Identity = 51/129 (39.53%), Postives = 68/129 (52.71%), Query Frame = -2
Query: 7669 KDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8049
+D C N+ CR+ CI C+G ++C DG DE TC C +FSC GG+ +C+ + WRCDG DC NG DEL+C TC F C D I R CD++ +CL+G DE +C
Sbjct: 24 EDSCGKNEFQCRDGKCIVSKWVCDGSRECPDGSDESPETCMSVTCRSGEFSC---GGRV--SRCIPDSWRCDGRTDCENGSDELDC---SPKTCSLDEFRCQDG-KCISRQFVCDQDWDCLDGSDEAHC 143
BLAST of SMED30010499 vs. UniProt/SwissProt
Match: sp|C0HL13|LRP2_PIG (Low-density lipoprotein receptor-related protein 2 OS=Sus scrofa OX=9823 GN=LRP2 PE=1 SV=1) HSP 1 Score: 87.8113 bits (216), Expect = 2.348e-15 Identity = 49/131 (37.40%), Postives = 70/131 (53.44%), Query Frame = -2
Query: 7639 CRNSICIDENKFCNGIKDCEDGFDE----EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8019
C N C+ + C+G+ DC D DE NT C P+ F+C GG+ C+ +YWRCD NDC +G DE NC + +C +F CD+++ IP++ CD + +C +G DEK C E P Q
Sbjct: 1033 CNNGRCVPLHYRCDGVDDCHDNSDEVQCGAFNT--SCAPSAFACGHGGGE-----CIPSYWRCDNHNDCVDGSDEQNCSSQAQTSCRADYFTCDNHM-CIPKNWLCDTDNDCGDGSDEKRCDLGETCSPTQ 1155 HSP 2 Score: 82.4185 bits (202), Expect = 8.617e-14 Identity = 51/127 (40.16%), Postives = 62/127 (48.82%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C N+ C N CI E+ C+G +DC DG DE + C QF C C+ YW CDGE DC NG DE + TC HF C N IP CD + +CL+G DE NCR+P
Sbjct: 28 CLGNEFRCSNGHCITESWRCDGTRDCLDGSDEIGCPPSTCGSTQFHCEN------EDVCIPLYWVCDGEEDCSNGADEHQRCPPGR-TCSSHHFTC-TNGECIPVEYRCDHSTDCLDGTDEINCRYP 146 HSP 3 Score: 80.4925 bits (197), Expect = 3.611e-13 Identity = 49/121 (40.50%), Postives = 62/121 (51.24%), Query Frame = -2
Query: 7678 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
CS Q C N CID C+G KDC DG DE C +QF C VG KC++N +RCDG +DC + DE++C C F C ++ IP S CD +P+CL G DE
Sbjct: 1151 CSPTQFHCPNHRCIDLAFVCDGDKDCADGSDESA-CVINCTDSQFKC--VGSN----KCISNTYRCDGVSDCSDHSDEIDCPTRPPGMCRQDEFQCREDGICIPDSWECDGHPDCLTGSDE 1264 HSP 4 Score: 74.3294 bits (181), Expect = 2.723e-11 Identity = 47/123 (38.21%), Postives = 61/123 (49.59%), Query Frame = -2
Query: 7660 NSICIDENKFCNGIKDCEDGFDEEINTCAQCEPN-QFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR----HP 8013
N CI + CNG DC D DE+ C+P+ +F C + C+ WRCDG NDCG+ DE NC+ C S F CDD T IP CD+N +C + DE++C HP
Sbjct: 3687 NYRCIPKWAVCNGFDDCRDNSDEQNCESLTCKPSGEFRC-------TNHHCIPLRWRCDGHNDCGDNSDEENCV---PRQCSESEFRCDDQ-TCIPSRWICDQNNDCGDNSDERDCEVMTCHP 3798 HSP 5 Score: 73.9442 bits (180), Expect = 2.937e-11 Identity = 45/129 (34.88%), Postives = 66/129 (51.16%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE-INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
CS ++ C N+ CI C+G DC D DE+ + C + ++ H V PP ++C+ W CDG+ DC + YDE NC + C G+ F C N IP CD +C + DE+NC++P
Sbjct: 2903 CSSDEFKCDNNRCIQMEWICDGDNDCGDMSDEDGRHHCENHNCSSYAFHCVNSAPPSRRCIPLSWVCDGDADCSDAYDEHQNCT---RRNCSGTEFRC-SNGLCIPNWFRCDRRNDCGDYSDERNCKYP 3027 HSP 6 Score: 70.4774 bits (171), Expect = 4.077e-10 Identity = 44/124 (35.48%), Postives = 62/124 (50.00%), Query Frame = -2
Query: 7678 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8043
FC Q C + C N CN +DC DG DE+ C +CE NQ+ C +++C+ W+CD NDCG+ DE + + TCL +F C N IP+S CD + +C + DE
Sbjct: 3550 FCRLGQFQCSDGNCTSSNFICNARQDCPDGSDEDAVLCEHHRCESNQWQC-------ANKRCIPESWQCDSLNDCGDNSDE-DSSHCARRTCLPGYFKC-ANGHCIPQSWKCDVDNDCGDYSDE 3664 HSP 7 Score: 67.3958 bits (163), Expect = 2.969e-9 Identity = 46/142 (32.39%), Postives = 65/142 (45.77%), Query Frame = -2
Query: 7669 ATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDD--------NVTLIPRSAWCDENPNCLNGEDEKNC 8064
A L C NQ C N CI E+ C+ + DC D DE+ + CA+ C P F C + C+ W+CD +NDCG+ DE C+G + CD+ N IP+ A C+ +C + DE+NC
Sbjct: 3584 AVLCEHHRCESNQWQCANKRCIPESWQCDSLNDCGDNSDEDSSHCARRTCLPGYFKC-------ANGHCIPQSWKCDVDNDCGDYSDE------PLQECMGPAYRCDNYTEFDCKTNYRCIPKWAVCNGFDDCRDNSDEQNC 3712 HSP 8 Score: 65.4698 bits (158), Expect = 1.341e-8 Identity = 45/122 (36.89%), Postives = 60/122 (49.18%), Query Frame = -2
Query: 7660 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK--NCRHP 8019
C + CI + C+G DC D DE TC PN C + + CV W+CDG+NDCG+G DE + L+ L + F CD+N I R C+ +C +G DEK NC P
Sbjct: 3803 CSSGHCIPDQMRCDGFADCLDASDEA--TCPTRFPNGAYCPATLFECKNHVCVQPSWKCDGDNDCGDGSDEELHLCLNITCDLTNRFRCDNN-RCIYRHELCNHEDDCGDGSDEKKENCLAP 3921 HSP 9 Score: 62.003 bits (149), Expect = 1.267e-7 Identity = 41/117 (35.04%), Postives = 52/117 (44.44%), Query Frame = -2
Query: 7669 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8019
C N CI C+G DC D DEE QC ++F C Q C+ + W CD NDCG+ DE +C + TC +F C IP CD +CL+ DE C
Sbjct: 3725 CTNHHCIPLRWRCDGHNDCGDNSDEENCVPRQCSESEFRCDD-------QTCIPSRWICDQNNDCGDNSDERDCEVM---TCHPGYFQCSSG-HCIPDQMRCDGFADCLDASDEATC 3830 HSP 10 Score: 60.4622 bits (145), Expect = 3.892e-7 Identity = 46/124 (37.10%), Postives = 57/124 (45.97%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS + C N CI C+ DC DG DE IN C Q +CH + C RCDGE DC + DELNC + CL + F C IPR CD +P+C + DE +C
Sbjct: 109 CSSHHFTCTNGECIPVEYRCDHSTDCLDGTDE-IN-CRYPVCQQQTCH-------NGACYNTSQRCDGEIDCRDASDELNCTQ----RCLRNEFQCGSG-ECIPRDYVCDHDPDCSDSSDEHSC 218 HSP 11 Score: 59.3066 bits (142), Expect = 9.946e-7 Identity = 42/136 (30.88%), Postives = 65/136 (47.79%), Query Frame = -2
Query: 7669 VKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEE---INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNV----TLIPRSAWCDENPNCLNGEDE-KNC 8052
V C N+ C + CI E C+G +DC+DG DE + + + C ++F C + +C+ W CDG+NDCG+ DE + + C F C ++ IP S CD + +C + DE +NC
Sbjct: 2857 VSPTCKNNEFQCTSGSCIPELWHCDGERDCDDGSDEPATCVYSPSTCSSDEFKCD-------NNRCIQMEWICDGDNDCGDMSDEDGRHHCENHNCSSYAFHCVNSAPPSRRCIPLSWVCDGDADCSDAYDEHQNC 2985 HSP 12 Score: 58.5362 bits (140), Expect = 1.538e-6 Identity = 57/181 (31.49%), Postives = 81/181 (44.75%), Query Frame = -2
Query: 7597 NGTVYECVHNNLLIKAT----LIVKD--FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNC--LNGEDEKNCRHPEVVIPNQETTYVVHANSSISL 8109
NG +C H A IV D C+ Q C + CI E+ CN I +C D DE CA C P F+C + +C+ ++RCD NDCG+ DE C N + + F+C +N +P CD +C N DEKNC +P+ Y+ ANS++ +
Sbjct: 2668 NGPECKCPHEGRWYLANNNKYCIVDDGKRCNSTQFTCLSGYCILESLKCNDIDECGDSSDELETLCAYHTCPPTSFTC-------ANGRCIQRHFRCDHYNDCGDNSDESGCRFRSCN--ITTEFSC-NNGKCLPLQLVCDGIDHCNDNNTSDEKNCAQ-HTCLPD----YIKCANSNVCI 2833 HSP 13 Score: 57.3806 bits (137), Expect = 3.160e-6 Identity = 44/125 (35.20%), Postives = 57/125 (45.60%), Query Frame = -2
Query: 7657 CRNS-ICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNCRHPE 8019
C NS +CI C+G DC D DE C C+ N+F C T G C+ W CDGE DC +G DE +TC F CD+N + + W CD + +C + DE H E
Sbjct: 2826 CANSNVCIPRLFLCDGDNDCGDMSDENPIYCVSPTCKNNEFQC-TSG------SCIPELWHCDGERDCDDGSDEPATCVYSPSTCSSDEFKCDNNRCI--QMEWICDGDNDCGDMSDEDGRHHCE 2941
BLAST of SMED30010499 vs. TrEMBL
Match: A0A4D6G2K6 (Plc (Fragment) OS=Schmidtea mediterranea OX=79327 GN=plc PE=2 SV=1) HSP 1 Score: 603.208 bits (1554), Expect = 0.000e+0 Identity = 291/293 (99.32%), Postives = 292/293 (99.66%), Query Frame = -2
Query: 6481 LCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGP 7359
LCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRC GQRSGP
Sbjct: 1 LCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCIGQRSGP 293
BLAST of SMED30010499 vs. TrEMBL
Match: A0A5K4F322 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 469.544 bits (1207), Expect = 0.000e+0 Identity = 261/732 (35.66%), Postives = 409/732 (55.87%), Query Frame = -2
Query: 6283 STVNINSVKPFHAGTYYCDAK--KNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGT----VIWRREDKKSFQIDVESRVGGET-QALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKL-DKNTCLGSHFACDDNVTLIPRSAWCDENPNC-LNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNV-----FLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTC----ETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQN--QCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERN---DPLCDKC 8409
S ++I +V+ +H G Y C A K+ A ++ + L VTP E+ RPG++ +FLC+L +G V W R D++ + E +G +ALLIV + + Y C A L V++ C+ R C + C++E KFC+G+ DCEDG+DE + C +C N+ +C + P + C +W CDGE+DCGN +DE NC+ D + C G+H+ C +I R+ CD P+C NG+DE C +P ++ P+ ET + H + +++L C P TI+WR+NWG L + + +++ T +CN + LT+ ++ P +G+YTCEA++ RA+A D+ + V +C+ P FN+AA F MCL C+CS TD C+S GY +SP+ LL++FK+ I+ +Y + D + + + + ++ D + P+V F+ SDF + GSW++SY Y + IN+RLFGES + +PGP VIL N +S+YWC ++ ++++ P G+ N++ I L E++ WFID C + + R + ++SDIK + R+KY+ +QT E FN V+H + + D G + IE+C CP Y GLSCEKC GY+K+ N +C C C +CD GNC C G+R+GP+C +C+ G+ R L++DC C+ N V C + CKC E N +P CD+C
Sbjct: 584 SFLSIMNVQKYHEGFYVCRALNLKDTAKLIVKIR---DLTVTPEEVKARPGTTVQFLCELSTITGMKGGKVFWMRTDRQDLRSGKEEVIGTNGGRALLIVKDVGRFDHNISYMCTDGYSKGYAKLYVQEVCAPGFRPCGSQQCLEETKFCDGVVDCEDGYDEIPSKCTECGVNENACGLMNDVKPLKNCYLQFWHCDGEDDCGNNFDESNCLAPSDYDQCNGTHYTCPQGDKMIARAFMCDSVPDCEPNGDDESECSYPSIIEPSGETHLIGHQSGNLTLTCVAYGRPPPTISWRYNWGHLREGVKHSINYTVINCNMAISHLTLTDLEPRASGLYTCEAVNRGRALAPDFLVNVAKGGICKAPQFNDAAWFDDMCLTCYCSNVTDTCTSVKGYRKSPH--------DLLLDFKTTPIILSTYNQ---DGSIEEGQGITEIDGDILRIPDVPNESTFIESDFNLSGSWVKSYGYDIIINLRLFGESLNYLPGPFVIL-NSTDQSIYWCPPNDRLLVHTIPTGYDNRIHIRLSERDKWFIDKECKQKIDQTIGRAGFMQIISDIKKIGFRVKYFRDQTTLELFNIKVEHAVKS-DVPGQ--WVGEIEECTCPNGYEGLSCEKCAPGYQKDPDNPLKCRPFCA--CDKCDTEGNCIECPGKRAGPRCQQCQDGYYRPDKSLLATDCQPCDMCGN-NLPYVVKKCHFNPQADDDYFCKCRISEDNKLLNPTCDRC 1294 HSP 2 Score: 261.151 bits (666), Expect = 0.000e+0 Identity = 374/1631 (22.93%), Postives = 659/1631 (40.40%), Query Frame = -3
Query: 2052 VINAYRNLCQLLMN------ETAKKRDLSENKNCNSLGTENIVD-GKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQN--TNNQLLVNRTENIILVKRNPNQIGLVYVTLKLT----NSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNF-PKWRIGK-----EGKLLEDRETFIRALMTAQHLSIGFPFDNG-FVKDVRLRDLTI---------EHAKWMNDLG-KIAPVEMCLCPKGNPG--------IKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKW----NKVSESTHLNVTHEMTEDK---------SSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEE---------------------------KPPRLAV-EISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENK-VYKIDIEVPTNVTTGVKLKGVIVG---------SRVENPFRFVIEENIKRNLSINSDQLKVSVDKPV-LEVNFLESARVRVLVNP-KIRTETFWYKV-----------NED----GSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEI--LNTTEPRNASIS------------------LYVPDPIKCPSQSWELVDPK-TKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFET--SVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSST-----------------INWKIVGGFSLDLHEGIYTCRINN-------SFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPIS-RSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISY-PK-ETSIYLKV-DGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIK-----------TTMGQQI---PVSELPGFTKEIT-PKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDN----------------------DKITFGGLIYHPADNSAVGVCKADYFNT--IMYSEPFSFKVIKKDRTE-TPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNT-KFNPYNNSGKYTFIATNKFGTDSKTILLPQH---NNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYP-ISLKWS---------PVNEYQLS--GATVESS-DYVTSLIYDKFDR-KINSGEYTCQAISHQGLLTKK--VQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETD 6293
++N + CQL ++ + AK + +C+ GT+ D G C+CK Y G C EC G+F++ +CL+C+CS KT C D+ L R G + ++ TN + R + VK N G+++ + L + +D+ Y +YGGK+SF + +L + + +E+ES ++GR WT+ +FN T+ E F E+ + WR+G + + R +R L+T ++ I N + +++L L+I E + + G APVE+C CP NP CE C + + +I + P D+ CG C C +C EG Y YD K +CQ + + TK R I V E I + C A S+RGG+ H+W N+ S ++ + D S IL K + D G Y C A S +E F+++V + PP L V EI D +Q+ + P+ + W+ + DAK ++I+ T + ++ I T + + G +G +R+ P E + I D + ++ P + V F++ V+V P K W K+ ++D S+ LP G ++D +L IW+A + H G Y G ++ +G +V +++T ++V PM E+ EP + + + PD C + +W +VD + K DIT V + SD+ ++ L+ G +RF+C IP+ S P + +K ++ S +P + C D N + SSV + + ++ + +S+ ++W+ +G F ++G +TC +NN + S VP+ + +R Y + T + + + I + + ++L I P+N+ +I I + YS L + D+ +L + +++ G S+ +EIS PK E ++ K+ + + + G ++ C + + + + ID I W ++ T + + P E FTK P+ L I R + I + T Y + + S +I + + V + G ++ +E T+Q+ C DA N+ + +W T I S+ ++L +N D + GL P + A CK T YS+ +V+ D E P+L + DPN P YP V C D N S +T ++ D ++ + P ++ +T N KF+P ++G YT + N + + K + +P + E+ I S +N+ + GD +++IC ++GHP P L+WS ++ + LS G ++S Y T++ +FD ++G + C A++ G + ++ V+VE + I+ L+E G +I ++G N N D
Sbjct: 1286 LLNPTCDRCQLAIDRGDELPDDAKCDTKPQPMDCHYYGTQLTSDNGDCQCKTGYTGVKCDECEEGYFNYMGQCLQCYCSGKTSSC---------------PGFGFDIWLGQRTEMGGLQRKDYTTNGNQRIEREMDNFYVKYPLNNKGIIHFGINLIPPKPTTLTQNDVTIYKYIYGGKMSFKIDSIKLNPNELTQREASVIVELESPRYGRAWTLATFNLMTQRYEVEFHENWWLGGWRLGTPLTSFDSPVSLTRNQLLRFLITTTNIGIITSSGNSESLNNMKLSGLSIQIGLPIKTNERQAFAKEQGLPKAPVEICDCP--NPAKSNERELSPSCEGCTDLSKQTIIRLEPLGEDVGCGGCLGDKCDECSEGEYIYDIYTGKRLDSCQKAVTIDTKSRRIIVKLREPINLLCKATSYRGGLVTHEWITPNNEYSSQPIISRQYLAKPDYYNRVNLTTYQSETILQIKFAKQEDAGEYTCIAKGIGSEVNETFYVVVIDPNAVSLPDVPEDDEINRKFPLPIPPIHDPPSLTVYEIKK---DPDNSQITLIGGQPEILDTHHTIWYSINGTPIDAK---TEITDTARGAFIVRLPIPYETIKSGNDSITGYFLGKDPVYTPQWTRMMEPVIIATEAD-----EITPDDI---INPPAEVTVPFMQPYIVQVRTKPGKEDISVTWKKIGPLPETITEKKDDDVKTLDSDSTLPEGTSQDKTNLNIWAAAEQHEGIYEGTVVRDRDGVEVKRIQTIIRVQPMTSGSKTELGLAEAAEPIESEVGDDDDQEEKEPPAWDFIVGGFTPDAKHCENPTWTIVDYQLNKTTDITDKVIRTSDENFRVNYPLTSGLYLRFQCKPIPKT-----------SPDPRIILKPIYDNPDSTFPTRLVCMDLNPEYDSSV--NLTSSSMSQEMISHAITSTEQKFIIRKGDIPPIRRLEMDWRRIGEFDPFNNDGYFTCFVNNTETNGSKTIKLPSDKVPIQLPEDPFTRLYIY------SPDTYVVPELDGSAKIMIPEGEQLRIYCVFEARPSNEFHGWRGTTSEIENNIKIEHRIYSSLAQT---DKANLSLDGQKLECVYGER-SKSLEISVIPKDERKLHAKILSDAFINDRLVGITGSDMNLTCDVKHTTR--KPATID-KIDWYVQYPNGIRRDIKHTQLADSLSTGPKGEWIYFTKLTEKPQGLNIFCVVRLED--IQKATQAYYSSPHISLWI------REPKVLVSIEGQDHLGIITGIETQTAQIKCIVKDAWRNKTVEEADIAWE-TKITSIQ--EELSNNNNERARPQPSPKDLPMLGAKALYDTMVIAGLRRQP-NWIARFRCKGTDLKTGAYGYSDFVKLEVLPGDTLEQVPKLRLISHTDPNSP-YPNKVECIDDNQNYESQVTWTRQDEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSN---VNEIFQDSGDRYVRLICTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHKVYVRINELSESG-----LIENQKGSNGTITCNAYD 2838 HSP 3 Score: 74.7146 bits (182), Expect = 5.197e-9 Identity = 80/355 (22.54%), Postives = 144/355 (40.56%), Query Frame = -3
Query: 870 IRNGTTDYASKTVVVNIIENELLH--PDLVFVTEYSKESPFYPQKLIC-KDKTGVSSKVQVVKETGINNEKVN---IYLTHNEGTLDWNSH---PFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETT---TPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSG------DLFVVKKPVLFSNYSQYYAAVQINEFDA-KTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKN-LRGRCIVIVQGKKYFSKYF 1874
++ G Y S V + ++ + L P L ++ SP YP K+ C D S+V ++ +E ++ I T N L W + F P D G Y C N Y +P T TP+ V+ S VNEI + + + +I C +YG P ++ + +G ++ + ++ + Y+ + +FDA +G + C + N+ + +R ++ + + V I L + ++ +GSN T+ C D + Y WE+E NG++ + +++ + L + P N LR RCI + +Y S F
Sbjct: 2568 LKTGAYGY-SDFVKLEVLPGDTLEQVPKLRLISHTDPNSP-YPNKVECIDDNQNYESQVTWTRQ----DEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSNVNEIFQDSGDRYVRLI--------CTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHKVYVRINELSESGLIENQKGSNGTITCNAYDGYLQFILPDATYTWEVERINGERVHPNLIADQIEMNHNQLHYYGLSPTANKLRIRCIALNDTARYISSDF 2908 HSP 4 Score: 67.3958 bits (163), Expect = 1.046e-6 Identity = 75/357 (21.01%), Postives = 145/357 (40.62%), Query Frame = -3
Query: 3255 ELVDPKTKPIDITHL-VRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKG---------KIKI----TIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLD--LHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLV---NGKITEPISRSKYYSKLVISEVKD-------------QDHLGIYEVRVKLENGTVISR-KIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEII-DANIVWKIKTTMGQQI 4223
E P+ P D+ L + + D +I + RFRC D+K K+++ T++ + I S YP +EC D+N + S V W + +P+D + T +++ + W G D + GIYTCR N ++S+ K V +P + + I S+ N IF+ + + + + +G+P + L+W + NG S LVI + H G+++ G +I R ++ + K +Y++++ ++ SG + +G ++ C+ + G I+ DA W+++ G+++
Sbjct: 2523 ERARPQPSPKDLPMLGAKALYDTMVIAGLRRQPNWIARFRCK---GTDLKTGAYGYSDFVKLEVLPGDTLEQVPKLRLISHTDPNSPYPNKVECIDDNQNYESQVTWTRQDEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSNVNEIFQD--------SGDRYVRLICTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHK---VYVRINELSESGLIENQKGSNGTITCN---AYDGYLQFILPDATYTWEVERINGERV 2862
BLAST of SMED30010499 vs. TrEMBL
Match: A0A5K4F4U5 (Uncharacterized protein OS=Schistosoma mansoni OX=6183 PE=4 SV=1) HSP 1 Score: 469.159 bits (1206), Expect = 0.000e+0 Identity = 261/732 (35.66%), Postives = 409/732 (55.87%), Query Frame = -2
Query: 6283 STVNINSVKPFHAGTYYCDAK--KNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGT----VIWRREDKKSFQIDVESRVGGET-QALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKL-DKNTCLGSHFACDDNVTLIPRSAWCDENPNC-LNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNV-----FLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTC----ETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQN--QCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERN---DPLCDKC 8409
S ++I +V+ +H G Y C A K+ A ++ + L VTP E+ RPG++ +FLC+L +G V W R D++ + E +G +ALLIV + + Y C A L V++ C+ R C + C++E KFC+G+ DCEDG+DE + C +C N+ +C + P + C +W CDGE+DCGN +DE NC+ D + C G+H+ C +I R+ CD P+C NG+DE C +P ++ P+ ET + H + +++L C P TI+WR+NWG L + + +++ T +CN + LT+ ++ P +G+YTCEA++ RA+A D+ + V +C+ P FN+AA F MCL C+CS TD C+S GY +SP+ LL++FK+ I+ +Y + D + + + + ++ D + P+V F+ SDF + GSW++SY Y + IN+RLFGES + +PGP VIL N +S+YWC ++ ++++ P G+ N++ I L E++ WFID C + + R + ++SDIK + R+KY+ +QT E FN V+H + + D G + IE+C CP Y GLSCEKC GY+K+ N +C C C +CD GNC C G+R+GP+C +C+ G+ R L++DC C+ N V C + CKC E N +P CD+C
Sbjct: 584 SFLSIMNVQKYHEGFYVCRALNLKDTAKLIVKIR---DLTVTPEEVKARPGTTVQFLCELSTITGMKGGKVFWMRTDRQDLRSGKEEVIGTNGGRALLIVKDVGRFDHNISYMCTDGYSKGYAKLYVQEVCAPGFRPCGSQQCLEETKFCDGVVDCEDGYDEIPSKCTECGVNENACGLMNDVKPLKNCYLQFWHCDGEDDCGNNFDESNCLAPSDYDQCNGTHYTCPQGDKMIARAFMCDSVPDCEPNGDDESECSYPSIIEPSGETHLIGHQSGNLTLTCVAYGRPPPTISWRYNWGHLREGVKHSINYTVINCNMAISHLTLTDLEPRASGLYTCEAVNRGRALAPDFLVNVAKGGICKAPQFNDAAWFDDMCLTCYCSNVTDTCTSVKGYRKSPH--------DLLLDFKTTPIILSTYNQ---DGSIEEGQGITEIDGDILRIPDVPNESTFIESDFNLSGSWVKSYGYDIIINLRLFGESLNYLPGPFVIL-NSTDQSIYWCPPNDRLLVHTIPTGYDNRIHIRLSERDKWFIDKECKQKIDQTIGRAGFMQIISDIKKIGFRVKYFRDQTTLELFNIKVEHAVKS-DVPGQ--WVGEIEECTCPNGYEGLSCEKCAPGYQKDPDNPLKCRPFCA--CDKCDTEGNCIECPGKRAGPRCQQCQDGYYRPDKSLLATDCQPCDMCGN-NLPYVVKKCHFNPQADDDYFCKCRISEDNKLLNPTCDRC 1294 HSP 2 Score: 257.684 bits (657), Expect = 0.000e+0 Identity = 374/1631 (22.93%), Postives = 658/1631 (40.34%), Query Frame = -3
Query: 2052 VINAYRNLCQLLMN------ETAKKRDLSENKNCNSLGTENIVD-GKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQN--TNNQLLVNRTENIILVKRNPNQIGLVYVTLKLT----NSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNF-PKWRIGK-----EGKLLEDRETFIRALMTAQHLSIGFPFDNG-FVKDVRLRDLTI---------EHAKWMNDLG-KIAPVEMCLCPKGNPG--------IKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKW----NKVSESTHLNVTHEMTEDK---------SSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEE---------------------------KPPRLAV-EISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENK-VYKIDIEVPTNVTTGVKLKGVIVG---------SRVENPFRFVIEENIKRNLSINSDQLKVSVDKPV-LEVNFLESARVRVLVNP-KIRTETFWYKV-----------NED----GSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEI--LNTTEPRNASIS------------------LYVPDPIKCPSQSWELVDPK-TKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFET--SVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSST-----------------INWKIVGGFSLDLHEGIYTCRINN-------SFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPIS-RSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISY-PK-ETSIYLKV-DGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIK-----------TTMGQQI---PVSELPGFTKEIT-PKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDN----------------------DKITFGGLIYHPADNSAVGVCKADYFNT--IMYSEPFSFKVIKKDRTE-TPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNT-KFNPYNNSGKYTFIATNKFGTDSKTILLPQH---NNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYP-ISLKWS---------PVNEYQLS--GATVESS-DYVTSLIYDKFDR-KINSGEYTCQAISHQGLLTKK--VQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETD 6293
++N + CQL ++ + AK + +C+ GT+ D G C+CK Y G C EC G+F++ +CL+C+CS KT C D+ L R G + ++ TN + R + VK N G+++ + L + +D+ Y +YGGK+SF + +L + + +E+ES ++GR WT+ +FN T+ E F E+ + WR+G + + R +R L+T ++ I N + +++L L+I E + + G APVE+C CP NP CE C + + +I + P D+ CG C C +C EG Y YD K +CQ + + TK R I V E I + C A S+RGG+ H+W N+ S ++ + D S IL K + D G Y C A S +E F+++V + PP L V EI + T + V P+ + W+ + DAK ++I+ T + ++ I T + + G +G +R+ P E + I D + ++ P + V F++ V+V P K W K+ ++D S+ LP G ++D +L IW+A + H G Y G ++ +G +V +++T ++V PM E+ EP + + + PD C + +W +VD + K DIT V + SD+ ++ L+ G +RF+C IP+ S P + +K ++ S +P + C D N + SSV + + ++ + +S+ ++W+ +G F ++G +TC +NN + S VP+ + +R Y + T + + + I + + ++L I P+N+ +I I + YS L + D+ +L + +++ G S+ +EIS PK E ++ K+ + + + G ++ C + + + + ID I W ++ T + + P E FTK P+ L I R + I + T Y + + S +I + + V + G ++ +E T+Q+ C DA N+ + +W T I S+ ++L +N D + GL P + A CK T YS+ +V+ D E P+L + DPN P YP V C D N S +T ++ D ++ + P ++ +T N KF+P ++G YT + N + + K + +P + E+ I S +N+ + GD +++IC ++GHP P L+WS ++ + LS G ++S Y T++ +FD ++G + C A++ G + ++ V+VE + I+ L+E G +I ++G N N D
Sbjct: 1286 LLNPTCDRCQLAIDRGDELPDDAKCDTKPQPMDCHYYGTQLTSDNGDCQCKTGYTGVKCDECEEGYFNYMGQCLQCYCSGKTSSC---------------PGFGFDIWLGQRTEMGGLQRKDYTTNGNQRIEREMDNFYVKYPLNNKGIIHFGINLIPPKPTTLTQNDVTIYKYIYGGKMSFKIDSIKLNPNELTQREASVIVELESPRYGRAWTLATFNLMTQRYEVEFHENWWLGGWRLGTPLTSFDSPVSLTRNQLLRFLITTTNIGIITSSGNSESLNNMKLSGLSIQIGLPIKTNERQAFAKEQGLPKAPVEICDCP--NPAKSNERELSPSCEGCTDLSKQTIIRLEPLGEDVGCGGCLGDKCDECSEGEYIYDIYTGKRLDSCQKAVTIDTKSRRIIVKLREPINLLCKATSYRGGLVTHEWITPNNEYSSQPIISRQYLAKPDYYNRVNLTTYQSETILQIKFAKQEDAGEYTCIAKGIGSEVNETFYVVVIDPNAVSLPDVPEDDEINRKFPLPIPPIHDPPSLTVYEIKKDPDNSQITLIGGQV-KPEILDTHHTIWYSINGTPIDAK---TEITDTARGAFIVRLPIPYETIKSGNDSITGYFLGKDPVYTPQWTRMMEPVIIATEAD-----EITPDDI---INPPAEVTVPFMQPYIVQVRTKPGKEDISVTWKKIGPLPETITEKKDDDVKTLDSDSTLPEGTSQDKTNLNIWAAAEQHEGIYEGTVVRDRDGVEVKRIQTIIRVQPMTSGSKTELGLAEAAEPIESEVGDDDDQEEKEPPAWDFIVGGFTPDAKHCENPTWTIVDYQLNKTTDITDKVIRTSDENFRVNYPLTSGLYLRFQCKPIPKT-----------SPDPRIILKPIYDNPDSTFPTRLVCMDLNPEYDSSV--NLTSSSMSQEMISHAITSTEQKFIIRKGDIPPIRRLEMDWRRIGEFDPFNNDGYFTCFVNNTETNGSKTIKLPSDKVPIQLPEDPFTRLYIY------SPDTYVVPELDGSAKIMIPEGEQLRIYCVFEARPSNEFHGWRGTTSEIENNIKIEHRIYSSLAQT---DKANLSLDGQKLECVYGER-SKSLEISVIPKDERKLHAKILSDAFINDRLVGITGSDMNLTCDVKHTTR--KPATID-KIDWYVQYPNGIRRDIKHTQLADSLSTGPKGEWIYFTKLTEKPQGLNIFCVVRLED--IQKATQAYYSSPHISLWI------REPKVLVSIEGQDHLGIITGIETQTAQIKCIVKDAWRNKTVEEADIAWE-TKITSIQ--EELSNNNNERARPQPSPKDLPMLGAKALYDTMVIAGLRRQP-NWIARFRCKGTDLKTGAYGYSDFVKLEVLPGDTLEQVPKLRLISHTDPNSP-YPNKVECIDDNQNYESQVTWTRQDEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSN---VNEIFQDSGDRYVRLICTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHKVYVRINELSESG-----LIENQKGSNGTITCNAYD 2840 HSP 3 Score: 75.0998 bits (183), Expect = 4.943e-9 Identity = 80/355 (22.54%), Postives = 144/355 (40.56%), Query Frame = -3
Query: 870 IRNGTTDYASKTVVVNIIENELLH--PDLVFVTEYSKESPFYPQKLIC-KDKTGVSSKVQVVKETGINNEKVN---IYLTHNEGTLDWNSH---PFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETT---TPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSG------DLFVVKKPVLFSNYSQYYAAVQINEFDA-KTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKN-LRGRCIVIVQGKKYFSKYF 1874
++ G Y S V + ++ + L P L ++ SP YP K+ C D S+V ++ +E ++ I T N L W + F P D G Y C N Y +P T TP+ V+ S VNEI + + + +I C +YG P ++ + +G ++ + ++ + Y+ + +FDA +G + C + N+ + +R ++ + + V I L + ++ +GSN T+ C D + Y WE+E NG++ + +++ + L + P N LR RCI + +Y S F
Sbjct: 2570 LKTGAYGY-SDFVKLEVLPGDTLEQVPKLRLISHTDPNSP-YPNKVECIDDNQNYESQVTWTRQ----DEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSNVNEIFQDSGDRYVRLI--------CTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHKVYVRINELSESGLIENQKGSNGTITCNAYDGYLQFILPDATYTWEVERINGERVHPNLIADQIEMNHNQLHYYGLSPTANKLRIRCIALNDTARYISSDF 2910 HSP 4 Score: 67.3958 bits (163), Expect = 9.950e-7 Identity = 75/357 (21.01%), Postives = 145/357 (40.62%), Query Frame = -3
Query: 3255 ELVDPKTKPIDITHL-VRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKG---------KIKI----TIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLD--LHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLV---NGKITEPISRSKYYSKLVISEVKD-------------QDHLGIYEVRVKLENGTVISR-KIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEII-DANIVWKIKTTMGQQI 4223
E P+ P D+ L + + D +I + RFRC D+K K+++ T++ + I S YP +EC D+N + S V W + +P+D + T +++ + W G D + GIYTCR N ++S+ K V +P + + I S+ N IF+ + + + + +G+P + L+W + NG S LVI + H G+++ G +I R ++ + K +Y++++ ++ SG + +G ++ C+ + G I+ DA W+++ G+++
Sbjct: 2525 ERARPQPSPKDLPMLGAKALYDTMVIAGLRRQPNWIARFRCK---GTDLKTGAYGYSDFVKLEVLPGDTLEQVPKLRLISHTDPNSPYPNKVECIDDNQNYESQVTWTRQDEPIDPAQITTTPNNAVLKWTHNGMDKFDPRIDAGIYTCRSVNPYSSSVKQVYIPYDLMVTDYETPITPEVRILSNVNEIFQD--------SGDRYVRLICTFYGHPPPEGGLQWSIDRFNGTRNSLSSHWLSMQGLVIQSGRHYWTTIGSPQFDALGRHTGVFQCTALNSIGQIIERDQVRVELHK---VYVRINELSESGLIENQKGSNGTITCN---AYDGYLQFILPDATYTWEVERINGERV 2864
BLAST of SMED30010499 vs. TrEMBL
Match: A0A4E0S494 (Basement membrane-specific heparan sulfate proteoglycan core protein OS=Fasciola hepatica OX=6192 GN=D915_000229 PE=4 SV=1) HSP 1 Score: 456.833 bits (1174), Expect = 0.000e+0 Identity = 260/754 (34.48%), Postives = 398/754 (52.79%), Query Frame = -2
Query: 6280 WTFKNLASNVETNLSKDAVIRHNSFN---STVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASG----TVIWRREDKKSFQIDVESRVGGET-QALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDK-NTCLGSHFACDDNVTLIPRSAWCDENPNC-LNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPN-VFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTC----ETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERN---DPLCDKCL 8487
W+F S E + D I S S +NI +V+ +H GTY C A A++ L VL + TL V P ++ RPG++ RFLC+L SG + W R D+K + E +G +ALLIV++ + T Y C ++ + V++ C R+C + C+ + FC+G DCEDG DE + C +C N+ C +GGK P + C YW+CDGE+DCGN +DE NC K + C G+ + C ++ I R+ CD +C NGEDE C P VV P+ ET ++++++ C V P I+WR+NWG L + ++ T DCN + + LT+ ++ P+ G+YTCEA++ R +A D+++ V LC PMFN+AA F MC+KC+CSG T +C S GY +S K ++G ++ + Y S + +K +F ++ + + VFL G++GSW+ SY + LK +RLFG + + + G V L +SLYWC + + Y+ G+ N + I + E+E WF+D C + R + +L +K + IR Y +QT FE + ++H + P + ++ IE+C CP Y GLSCEKC GYE++ + + C ECD++GNC C+G RSGP+C +CK GF R Y L+ DC C+ +Q +V C+ + CKC +E DP CD+C+
Sbjct: 563 WSFIPKGSEEEVLVHPDVNIERPSATPATSFINIANVQKYHEGTYVCRALNMKATSELVVLTR-TLSVNPEQLDARPGTTVRFLCELSAVSGLDGGKLYWMRTDRKDLRPGKEEVIGSTGGRALLIVHSVDWTDHNTTYMCTDGVSSAQSRIFVREACGPGFRSCESGGCVSSSVFCDGKNDCEDGSDENVAKCNECGANELRCEEIGGKKPLRSCFLQYWKCDGEDDCGNNFDEENCPPPTKPDRCNGTQYICPESNKPIARAFLCDGVADCEPNGEDENECSLPVVVEPSGETQMFGKRSTNLTMTCIVHGKPPPAISWRYNWGGLRDGVKHVINHTVVDCNTVISHLTLIDLEPEAGGLYTCEAVNKGRTLAPDFSVNVAQGGLCHPPMFNDAAWFEDMCIKCYCSGVTQNCRSVQGYSKSSTDKYWIISEG-------SSVIRI-YRDSEVEETTKGLEFTPRLIRYKGSESGLVFLEGPLGLNGSWITSYGHNLKFTVRLFGTTTERLKGAMVALHGPE-ESLYWCPPTDRVLFYAMNVGYENDISIRINERERWFLDPNCTQRLSEATGRAQFMNLLKMVKRVGIRTNIYDDQTSFELQDLRLEHSV--PSDL-PGVWVAEIEQCECPPGYEGLSCEKCAYGYERHPTEPGLCRLQCACDECDEHGNCLTCSGNRSGPRCQQCKTGFYRPYESSLADDCIPCDRCAQGAPHVVKE-CVDSMLPAEDPFCKCQAKEDGKLVDPDCDQCV 1302 HSP 2 Score: 291.967 bits (746), Expect = 0.000e+0 Identity = 253/938 (26.97%), Postives = 409/938 (43.60%), Query Frame = -3
Query: 3777 ENKNCNSLGTEN-IVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTL-NSADIKLDVVLSNRQISG--VYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKL----TNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPK-WRIGKEGKLLED-------RETFIRALMTAQHLSI--GFPFDNGFVKDVRLRDLTIE----HAKWMNDLGKIAPVEMCLCPKGNP------GIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNV---------THEMTEDKSSNL----------ILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIV-------------EEKPPRLAV---EISVTT-----------IDKVK-TQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVG-SRVENPFRFVIEE-NIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNP-KIRTETFWYKVN---ED---GSEHP--LPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRP---VV-----------NKEILNTTEPRNA-----SISLYVPDPIKCPSQSWELVDPKTKPI-DITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFE--TSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSST--------------INWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVP 6224
E C + GT++ V G+C+C+ Y+G C +CS G+F F K+CL C+CS +T C+ SD++ F N+TL + D+ L+ G + TN + + + ++V+ + GL++ L + T S DI Y LYGG++SF + + S IEI+S +FGR+WT +FN +T+ E F E+ +P WRIG E R +R L TA +SI F G KD+RL L I+ A + APVE+C CP+ + + CE C + + P PD+ C C P+C +C G +KY+ ++ TC+ G+EM ++ R + + + + C S+ GGI H+W S S V +E DK S + I++ + SD G+Y C A S+ ++ V EEK L V IS TT D K T V TV +P T ++ WF K + T V ++D+ + ++ + G VG S V NP+ + + I + + +P + V F++ ++V V P K W ++ ED E P LP G+T+D +L+IW+ + H G Y G +I + ++ + TV+V PM P V+ E + + P+ + + PD C +W +VD +T I D+T V ++S +D L+ G +RF+C +P++++ G+IK I S P + + ++ S +P + C D N + PS V VD+ + S+ ++W+ +G F + G +TC +NNS S SK++ VP
Sbjct: 1320 EPSPCEAAGTKSGTVAGECECQTGYRGDRCEDCSDGYFRFHKRCLGCYCSGQTSSCRMSDRHYFWNVTLADPQGFDFDIWLAQGSAHGNLLRSDFTTNGERRLEQVNGDVIVRHPILERGLIHFVLNIHHTQTGSQTDSDIPIYRNLYGGQMSFRLNSPNLDPQKVTQSQAGVVIEIDSPRFGRVWTQATFNVETQRYEVEFNENWWPGGWRIGTTDAFGERGPQQGLLRGQLLRVLGTASSISIVTSSGFSEGL-KDMRLSGLAIQVALPSAPSSDPDAPFAPVEVCDCPEPSKPEERALSLSCEGCRDPRKRTTVRLQPGGPDLECEECRGPNCDECASGLFKYNVYTGRSLQTCRPGLEMDSEQRYVVIRTGSPLTLVCRVTSYLGGIVTHEWVAPSSSDPDRVEISRRPILRPNEPGIDKDSQMFNQSIHRSEAIISISSAEKSDSGVYSCRASGIGSSLDRPTYVEVLDETDGIPLTHPDEEKLGALNVIQPSISPTTPFLSKYTFESEPDNPKVTVVKGTVKNPSDLTANQLVWFASNGSAFHIK--PDVYNTETGEVVVRLDVPYSSVLSGKESINGYFVGNSPVFNPYWTPMRKPEIAFPTADEVSAKDIVPPEPEVRVPFMQPHTIKVQVTPGKTPIHVIWTRIGSLPEDFRKWEELPGALPEGMTQDETNLIIWAGTEQHEGVYEGLVIRDSDQQEIARFNVTVRVDPMVPDSKVILPPGKADDLPDKDENVTSVTPKPGVTWDFVVQGFTPDAEYCEYPTWTMVDYQTNTITDVTERVHRLSPSSFRVDYPLTSGIYLRFQCQPVPKLNLTGEIKFNISSPDPRIVFQTLYDDPNSTFPTRLICLDLNPSRPSDVNISSDSMTVDRIRDALSVPSTVKVHRPGDVPIRQLELDWRRIGDFDPFIDSGKFTCFVNNSETSGSKTITVP 2254 HSP 3 Score: 90.1225 bits (222), Expect = 1.286e-13 Identity = 92/404 (22.77%), Postives = 166/404 (41.09%), Query Frame = -3
Query: 870 FSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKT--VVVNIIENELLH--PDLVFVTEYSKESPFYPQKLIC-KDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNS---HPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPM---NEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP----MIDVEIVYE--AKSGDLFVVKKPVLFSNYSQYYAAVQINEFDA-KTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLS-ESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYF 2024
SG+ E R P L T + N V G ++ + V E R R ++ ++ ++++ V + ++ + L P L + S E P+YP ++ C D S+V ++ G ++ + N L W F P D G Y C TN + LP+ P+ + ++ S VN+I E + + ++ + + C+YYG AP VE + E +G + + + Y++ + +FDA +G Y C + + + + I+ + ++V I+ L+ ++ T+G N + C D + +Y WEIE NG + N +L+ E + D N+ R RC+V +Y S F
Sbjct: 2541 ISGIDEDRIPNSYEIPWLGTREDNGILVVGGLRVREEWVG-----EFRCRAKSPVSNASAESDWVRLEVLTGDTLMRVPKLRLEAKSSPEFPYYPTQVNCIDDNEEFPSEVTWTRQEGTIPPN-DLEVKPNSARLTWTHDGVEKFDPQRDAGVYVCRATNAFSSSVRQFYLPQDMMPKEPIPISDHKIEITSDVNQIFYENGDRYMRVVQNSPFELLCSYYGHPAPPGGLQWSVERINELGQSAGSPSIAMLGLRVRSGRHYWSEIGTPKFDAMGRHAGVYRCEALSQDGRVIKQSLIRVELHKVNVRIQELNDAGFIEATEGDNGAITCNAYDGYFEFILPEAKYSWEIERMNGDRVNPNSLATEVNIEDNHIKYTNINPAANAFRARCVVHNDTARYVSADF 2938 HSP 4 Score: 70.8626 bits (172), Expect = 8.189e-8 Identity = 74/332 (22.29%), Postives = 130/332 (39.16%), Query Frame = -3
Query: 3102 VEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVG--GFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITE-------PI---------SRSKYYSKLVISEVKDQD-HLGIYEVRVKLENGTVISRK-IEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDV 4037
+E K E YP + C D+N PS V W + + N + +S+ + W G F G+Y CR N+F+S+ + +P I ITS N IF + + V + E+ +G+PA L+W V +I E P S Y+S++ + H G+Y ++G VI + I + K + +++ +N +G + EGD ++ C+ E + +A W+I+ G ++ + L I +K + N N++ +C + Y S F +
Sbjct: 2618 LEAKSSPEFPYYPTQVNCIDDNEEFPSEVTWTRQEGTIPPNDLEVKPNSARLTWTHDGVEKFDPQRDAGVYVCRATNAFSSSVRQFYLPQDMMPKEPIPISDHKIEITSDVNQIFYENGDRYMRVVQNSPFELLCSYYGHPAPPGGLQWSVE-RINELGQSAGSPSIAMLGLRVRSGRHYWSEIGTPKFDAMGRHAGVYRCEALSQDGRVIKQSLIRVELHK---VNVRIQELNDAGFIEATEGDNGAITCNAYDGYF--EFILPEAKYSWEIERMNGDRVNPNSL-ATEVNIEDNHIKYTNINPAANAFRARCVVHNDTARYVSADFGFRI 2942 HSP 5 Score: 70.4774 bits (171), Expect = 1.245e-7 Identity = 90/376 (23.94%), Postives = 136/376 (36.17%), Query Frame = -3
Query: 2052 VSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSV-------------VNWKKLIEDNDKITFGGLIYHPADNSAVG--VCKAD--YFNTIMYSEPFSFKVIKKDR-TETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNT-KFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKII--------CVYFGHPYPI-SLKWS--PVNEYQLS---------GATVESSDYVTSLI----YDKFDRKINSGEYTCQAISHQGLLTKK--VQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETD 3044
V VE T + C D + +W T + + + W EDN + GGL VG C+A N S+ +V+ D P+L + P P YP V C D S +T + KP + +T + KF+P ++G Y ATN F + + LPQ P + +N GD ++++ C Y+GHP P L+WS +NE S G V S + S I +D R ++G Y C+A+S G + K+ ++VE + I LN+ G I +G+N N D
Sbjct: 2504 VQGVEAQTLHLKCAVTDLWRQKPARNAHITWE-TRVKPISGIDEDRIPNSYEIPWLGTREDNGILVVGGLRVR---EEWVGEFRCRAKSPVSNASAESDWVRLEVLTGDTLMRVPKLRLEAKSSPEFPYYPTQVNCIDDNEEFPSEVTWTRQEGTIPPNDLEVKPNSARLTWTHDGVEKFDPQRDAGVYVCRATNAFSSSVRQFYLPQDMMPKEPIPISDHKIEITSDVNQIFYENGDRYMRVVQNSPFELLCSYYGHPAPPGGLQWSVERINELGQSAGSPSIAMLGLRVRSGRHYWSEIGTPKFDAMGR--HAGVYRCEALSQDGRVIKQSLIRVELHKVNVRIQELNDAG-----FIEATEGDNGAITCNAYD 2868
BLAST of SMED30010499 vs. TrEMBL
Match: A0A4S2KYA6 (Uncharacterized protein OS=Opisthorchis felineus OX=147828 GN=CRM22_010460 PE=4 SV=1) HSP 1 Score: 450.284 bits (1157), Expect = 0.000e+0 Identity = 259/757 (34.21%), Postives = 395/757 (52.18%), Query Frame = -2
Query: 6280 WTFKNLASNVETNLSKDAVI-RHNSFNST--VNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGA----SGTVIWRREDKKSFQIDVESRVGGET-QALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNC-LNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKM--YQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTC--ETSAM--RPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYE--KNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERN---DPLCDKCL 8487
W F ++E +L + I R + ST + I +V+ +H G + C + A LNV + L V P E+ RPG+SARFLC+L G + W R D++ + E +G + LLIV + + E N T YEC + A + V+D C RAC + C++ +FC+G +DCEDG DE ++ C +C PN++ C + GK P +KC YW CDG++DCGN +DE NC + + + C G+HFAC LI RS CD +C +G DE C P ++ P+ ET + +++L C V P INWRFNWG L + + ++ T DCN++ + LT+ NM P +G+YTCEA+S +R+MA D + V +C P FN+ A F CL+C+CSG TD C SA GY +SP K + + V Y I + + + + Y ++ D +L +FG+ G W+ SYSY +K+ IR +G + PG V L K++YWC + N ++ + +G+ N+L I L E++ WF D C S++ R + +L D++ + IR++ + +QT E + +++V S + + +E+C+CP Y GLSCE C GY+ ++ +C +C C +CD GNC C G R GP+C C+ GF R + DC C + +V C + +CKC RE DP CD+C+
Sbjct: 557 WYFLPSEDSIERHLPQGVSIGRPAATPSTSFLTIKNVQKYHEGEFLCRSFGMERRARLNVKLR-ALSVNPEEVDARPGTSARFLCELSVVPGLDGGKLFWMRTDRRDLKPGKEEVIGSTGGRTLLIVKSLSWEDNSTTYECSDGVNSVTARIFVRDDCPPGYRACNSRGCVESWRFCDGKQDCEDGSDELLSKCDECSPNEYVCRMLDGKKPLRKCYLQYWHCDGDDDCGNNFDEANCPLPSEYDKCNGTHFACPGQSKLISRSFVCDGVADCEADGADEHECSDPVIIEPSGETHLFGIQSKNLTLTCAVYGHPPPAINWRFNWGGLRDDVTYVMNTTVVDCNKVISQLTLTNMEPQASGLYTCEAVSKRRSMAPDIAVNVAKGGMCTVPFFNDGATFADECLRCYCSGVTDKCQSAKGYVKSPVNK--------MWKLTGTTSVIVLYNDDIGEATTDGLQITDNLVRYTNYPED-EAYLEGEFGLQGPWVTSYSYNMKVKIRQYGPFTNFFPGALVALHGKE-KTIYWCPHPNQILLNTAEDGYENELSIKLNERDRWFEDRKCSRRLSSLDGRSAFMNILRDVQTIGIRVRNFEDQTSTEVQDVQLEYVTP---SGRTDAWVTEVEQCDCPVGYEGLSCESCALGYQPDPDEPGKCQPRC--VCDKCDAQGNCINCPGNRVGPRCQHCQTGFYRPERSTMDEDCVPCEKCAAAPAHVVKE-CAGSTLPEQHYLCKCEVREDGKLVDPDCDQCV 1296 HSP 2 Score: 327.02 bits (837), Expect = 0.000e+0 Identity = 390/1649 (23.65%), Postives = 674/1649 (40.87%), Query Frame = -3
Query: 1932 AKKRDLSENKNCNSLGTENIV-DGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTL-NSADIKLDVVLSNRQISGVYE--AQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKL----TNSTVPDDIHKYTLLYGGKISFTFEY-NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPK-WRIGKEGKLLEDRETFIRALMTAQHLSI-------GFPFDNGFVKDV---RLRDLTIEHAKWMNDL---GKI-------APVEMCLCP-KGNPGIK-----CEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKW------NKVSESTHLNVTHEMT----------EDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEK----PPRLA---------VEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTEN-----KVYKID---------IEVPTNVTTGVK--LKGVIVGSR-VENPF-----RFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNP---KIRTETFWYKVNE--------DGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTT-----EPRNASISL---------YVPDPIKCPSQSWELVDPKTKPI-DITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFE--TSVYPVSMECRDNNTAHPSSVVWYFKGKPVDK---NLVIN-----------TNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQAL-SIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISR--SKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIE--------ISYPKETSIYLKVDGINPSGNMTI-LEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSE-----------------LPGFTKEITPKEL----KISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSV----------------VNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFS-FKVIKKD-RTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNT-KFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAE----LSIFSTSPVINDK-----IRIQGDTSLKIICVYFGHPYPI-SLKWS---------PVNEYQLSGATVESSD-YVTSLIYDKFDRK-INSGEYTCQAISHQGLLTKKVQ--VESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIR-------HPGMTNYSLLTTDKVNAKFEVTG 6245
AK + E CN+ GT++++ +G C CKENY GP C +C PGF ++ CL+C+C+ ++ C+ S ++ F N+T+ + A + D L+ R I G E Q + RT ++V+ + G++++ +++ +S +DI Y LYGG++ F + N DL K L + +E+ES+++GR+WT +N+ +T E F E+ +P W++G +E F + L Q L + +G K + ++ L+I+ A+ +N G + APVE+C CP +P + CE C VI ++P PD VC C G +KY+ + + +CQ+G+ + T+ R+I V K + +R+ C + SHRGGI +H+W + + + + H + E S I + D G Y C A + A ++IV E PP + ISV K + V+ T++S PTD V +K + E + ++ K+ ++D + VP ++G VG NPF R + E+ K + N ++ +E+ F++ +RVL P KIR + W ++++ D P G+ +D NDL+IW+A + H G Y G + D+G ++ + T++V P+ P + T +P + ++ + P+ C W L+D + K + D+TH V+++S ++ L+ G +RF+C+ +P + G+IK I S P + + +E S +P + C D N + PS V DK LV + + ++W+ +G F G YTC +NN+ S +++V +PD K I I S + ++ S+ VT+ RL + PA+D L W + R + YS LV I E + L+ TV + ++ + E + +KV G+ I G ++C +K V+G I I W ++ G++ VS+ G TK T ++ + A L +Y + S + + + +++ L V E T ++ C A D NR + V+ SW T + + W E ++ + GGL P D + + S ++ +V+ D + TP+L + P+ P YP C D S ++ + V KP I + N +F+P + G YT A N + + K I P PA L ST ++ D R+ +++CV++GHP P L+W+ ++ + G V+S Y L FD K ++G Y C A+ G + ++ Q VE + +++ L + G VI +++G + V + D N + WE+ HP M +++ T+ + E TG
Sbjct: 1309 AKCEEQPERLECNTEGTQSVLGNGDCVCKENYDGPRCDQCKPGFIKYKNSCLECYCAGQSPSCQLSKEHVFWNVTMEDPAGMTFDFWLARRSIHGGLERVEDAVTEQPRLERTPTELIVRTPVLERGIIHLAVQIKPPPVDSPTTNDIPIYRYLYGGQMFFRLDAVNLDLSK-LKAEEARVVVELESERYGRVWTQAVYNKALQTYEVQFDENWWPGGWKLGTP---IEGHGQF-QGLTRGQLLRVLATTSSISIVTSSGLSKGLAGMKISGLSIQLAQSVNQRPTPGGVDPNTLPPAPVEVCDCPMPSSPDERELSPSCEGCKDTSLRTVIRMNPYGPDFVCSTLLGSKFDGCPSGQFKYNVYTGERYDSCQLGLTIDTRQRVIVVTKGDPVRLVCESTSHRGGIGVHEWIPPVSSARTANESRRRIIHTPSLAIRALPSDAESHRSEAIFEIENANKQDEGEYRCRATVIGTMAELPIYLIVREPDEPMPPAIVDAEDPLNSKFWISVPPGAKAPSAVSYTLES--DPTDDSVTRATIKMRDPADLENHQLVWLSPNGSQIVTKLERMDLFRGEAQFLLHVPHAQLEAQPEDVQGYFVGKNPASNPFWTPIGRPKVMESAKDRVKSND----ITAPPSTVEIPFMQPETLRVLKKPGKSKIRVD--WKRLSDIPDKPEPSDVIPSRFPEGMIQDGNDLVIWAAAEQHEGVYEGVVYRDDDGKELKRFNVTIRVQPLNPGKTTHLAPGTAADLPQPTDDDVTTPVWDFVVQGFTPEAEYCEEPKWMLIDYQKKTVTDVTHKVQRLSPSSFRVEYPLTSGIYLRFQCTPVPWSKLFGEIKYNITSDDPRIVFETYYENPNSTFPTRLVCYDLNPSRPSDVEIASDAMGPDKLKEALVTSPLGREQDPHGLSKVKVELDWQRIGNFDPFSDSGGYTCFVNNTEASGTRTVQIPDEKVPVKVPEDPFSRIYIYSPDTFVSQEVDGRSSVEVTEGDRLRLYCHYDARPASDF-LGWGSDSSEVNANMRFTHRPYSSLV----------EIGEANLDLDGKTVSCKTVDKEKVVELKVISKTERAYEVKVTSEYMVGDRIIGTTGGDLDLQCE-VKQVRGVPVAI--QRIDWFVQYPNGRRHAVSKSKLAQNVQLEDNGSKLRFSGLTKRSTGAQIFCVARPVDATSALQAY------------HPSPSVELWIRSPKLLAEIQPLAR--PGVVVGPEAGTIRLYCTATDKWHNRTVENVQISWE-TEVRPEYIPDGGELKIRPSPHDIPWLLKQEIHNSLVVGGLRRPPEWIGRFRCTVTDPLSNVSTSSDWAVLEVLAGDVASRTPKLRIIAESRPSFPFYPTRAECVDENKEYPSEVSWIRNEDAMVPENVEQKPSSATIIWKVNGIEEFDPRKHGGIYTCKAVNPYSSAYKQIQFPLDVMPKEPIPAADHKLLITSSTHEILRDTDEIPYTRVIQGQPFELLCVFYGHPPPAGGLQWTVKRTGTATDGLSRMLMQGLQVKSGRLYWAQLSASSFDAKGKHTGLYQCAALDSTGNVVQQTQVRVEMYKVNVKVKELTDSG-----VIEREEGSDGVITCSAYDGYLNFILPDATYTWEVEKYTGERVHPNMLANTVVITEN---RIEYTG 2907 HSP 3 Score: 80.8777 bits (198), Expect = 8.252e-11 Identity = 101/436 (23.17%), Postives = 178/436 (40.83%), Query Frame = -3
Query: 3009 KTKPIDITHLVRKVSDKEIIIDRELSIGQVI-RFRCSVIPEIDMKGKIKITIDSKFPVVEI----------------KKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVG--GFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALS-IFVTKKQRLEITAELFGNPANDSQLEWLV--NGKITEPISR-----------SKYYSKLVISEVKDQD-HLGIYEVRVKLENGTVISR-KIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEII-DANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVI 4208
+ P DI L+++ +++ + I RFRC+V + ++ S + V+E+ + + YP EC D N +PS V W + V SS+TI WK+ G F H GIYTC+ N ++SA K + P + ITSST+ I + + V + Q E+ +G+P L+W V G T+ +SR Y+++L S + H G+Y+ G V+ + ++ + K + +KV + SG + EG + CS + G + I+ DA W+++ G+++ + L T IT ++ + +R +++ +C + Y S F V++ + + R I+ +EED Q I
Sbjct: 2545 RPSPHDIPWLLKQEIHNSLVVGGLRRPPEWIGRFRCTVTDPLS-----NVSTSSDWAVLEVLAGDVASRTPKLRIIAESRPSFPFYPTRAECVDENKEYPSEVSWIRNEDAMVPENVEQKPSSATIIWKVNGIEEFDPRKHGGIYTCKAVNPYSSAYKQIQFPLDVMPKEPIPAADHKLLITSSTHEILRDTDEIPYTRVIQGQPFELLCVFYGHPPPAGGLQWTVKRTGTATDGLSRMLMQGLQVKSGRLYWAQLSASSFDAKGKHTGLYQCAALDSTGNVVQQTQVRVEMYK---VNVKVKELTDSGVIEREEGSDGVITCS---AYDGYLNFILPDATYTWEVEKYTGERVHPNML-ANTVVITENRIEYTGLSREASNFRAKCVAFNQTARYYSTPFGFRVHRVSGEPAKRPTD-IEPGNTRWIEEDGKQPI 2967 HSP 4 Score: 73.1738 bits (178), Expect = 1.907e-8 Identity = 74/350 (21.14%), Postives = 148/350 (42.29%), Query Frame = -3
Query: 870 TTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKV----NIYLTHNEGTLDWNSH---PFSPFFDQGTYKCIGTN----RYKQ-RFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENK-KTLEIINGNKLLISCNYYGTEAPMIDVEIVYE----AKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQ-SGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYF 1862
++D+A V+ + + P L + E PFYP + C D+ +K + + I NE N+ + T+ W + F P G Y C N YKQ +F V+P +E + + S +EI + ++ +I G + C +YG P ++ + A G ++ + + + Y+A + + FDAK + +G Y C + ++T ++ + ++ + ++V +K L + ++ +GS+ + C D Y WE+E+ G++ + L+ + V + + + + N R +C+ Q +Y+S F
Sbjct: 2592 SSDWAVLEVLAGDVASRT--PKLRIIAESRPSFPFYPTRAECVDE----NKEYPSEVSWIRNEDAMVPENVEQKPSSATIIWKVNGIEEFDPRKHGGIYTCKAVNPYSSAYKQIQFPLDVMP--KEPIPAADHKLLITSSTHEILRDTDEIPYTRVIQGQPFELLCVFYGHPPPAGGLQWTVKRTGTATDGLSRMLMQGLQVKSGRLYWAQLSASSFDAKGKHTGLYQCAALDSTGNVVQQTQVRVEMYKVNVKVKELTDSGVIEREEGSDGVITCSAYDGYLNFILPDATYTWEVEKYTGERVHPNMLANTVVITENRIEYTGLSREASNFRAKCVAFNQTARYYSTPF 2933
BLAST of SMED30010499 vs. Ensembl Cavefish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 211.075 bits (536), Expect = 1.359e-63 Identity = 172/627 (27.43%), Postives = 269/627 (42.90%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTV-HQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ------------------------SPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKV-ILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCKC--TGRERNDPLCDKC 8040
C +Q C+N CI + C+G +DC DG DE + T + CEPN+F C + +C WRCDG+NDC + DE++C ++ + C F C + IP S CDE P+C + DE C P V P +++ + + ++ C+ V VPT I WR NWG +P T+ + G+ LTI ++ G YTCEA++ K A+ + C + FN C+ CFC G T +C S Y +P P TST+ +L+N + ++ V ++ D++S P FL G+ ++SY +L+ + + + P K IL NG+ L + + +P+ NQ +I E ENW T S R ++L L++++ + IR Y + + +D G P+ +E+C CP Y+GLSCE C +G+++ + + C NC CD +G+C C GP+C +C+PG+ DC C C + S+ C D+ GQ C+ TGR C+KC
Sbjct: 196 CRGDQAMCQNGQCISRDYLCDGERDCTDGSDEMKCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCKDNSDEMDCPVRRPGDACAPEQFVCLSDRACIPASYQCDEEPDCADRSDEYGCAPPTVTSPPEDSVTAMRGGT-VTFTCKAVGVPTPIITWRLNWGHIPVSSRITMTSENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLVFAIPDGVLTLNRVPSNCPEGHFNIGGR----CMPCFCFGITKNCQSTGRYRNQISLRFDEEENFKGVNVSYPIRPGAPPPLTSTQ---ILINPEEEEFQLVDLSRRFLDLDS------------HWTLPRQFL-------GNKVDSYGGFLRYKVSYSLQREGSEPDNKADILLMGNGQRLVYRRGSPT-----SPD-IKNQREIKFTE-ENW--QHTSGRSVTREELLMTLANVESIRIRTVYDNRMVSVGLSDVVMDTTSVEFSLQGQ----PKDVEECRCPPGYSGLSCESCSSGFDRVPGGRYLGTCAGCNCHGHASACDPVSGHCLSCQHNTEGPQCDKCRPGYFGDPSRGRHDDCKPCPCPYTETSRRFSDTCFLDVDGQATCDACRPGYTGRR-----CEKC 764 HSP 2 Score: 55.4546 bits (132), Expect = 1.359e-63 Identity = 78/326 (23.93%), Postives = 124/326 (38.04%), Query Frame = -3
Query: 5301 IVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLL-VNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFT--FEYNT-----DLLKDLVFSDVTPFIE--IESKKFGRLWTVLS--FNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGF----VKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6188
+ +G+ C N GP C EC PG F CL+CFC T++C S R Q+ G N QL + N + +Q G ++ + NS VP DI+ + + YGG++ + FE+ + D D+V F+E ++K R+ TV++ F E WR +G+ RE + AL L I + + + D+ + D+ + H+ + VE C CP+G G C+ C +VGY + + G C+ DC
Sbjct: 780 VPNGEPLCLANVAGPLCDECKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTR-------------------DQVRG-----GVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNS-VPSDIYYWVMPESFRGDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQGNGIFLEHSSQTKPLPRVPTVVTVPFRESA--------------WRRA-DGQPCT-REHLLMALADVAVLMIRATYADKMSESSISDIHM-DIAVPHSTGNE---RAMEVEECACPQGYRGPSCQEC-EVGYTRTGS------GLYLGTCEKCDC 1053 HSP 3 Score: 82.8037 bits (203), Expect = 8.574e-27 Identity = 93/385 (24.16%), Postives = 151/385 (39.22%), Query Frame = -2
Query: 6238 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFV-GKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 7356
C+ F+ +A CL+CFC G T C+S+ + + L ++ I E K +++ +S+ V +Y + V P F G + +Y L+ +R +S + P V+LQ G ++ + ++ + P + S W + + R +L L+D+ L+IR Y + + +D I P S GN A +E+C CP Y G SC++C GY + C K C + ECD + G C +C +GP+C C PGF YG ++ C C C + + C D GQ P C+ C P A R+C++
Sbjct: 799 CKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTRDQVRGGVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNSVPSDIY--YWVMPESFR-------GDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQ---GNGIFLEHSSQTKPLPRVPTVVTVPFRESAWRRADG-------QPCTREHLLMALADVAVLMIRATYADKMSESSISDIHMD--IAVPHSTGNE-RAMEVEECACPQGYRGPSCQECEVGYTRTGSGLYLGTCEKCDCHGHASECDSETGACLQCLHNTAGPRCDSCLPGF---YGNPVTGGIHACRPCPCL----GSAPAKSCYLDSDGQ--------------PTCNNC---PPGYAGRRNCER 1137 HSP 4 Score: 60.4622 bits (145), Expect = 8.574e-27 Identity = 37/104 (35.58%), Postives = 47/104 (45.19%), Query Frame = -3
Query: 5922 KNCNSLGTENI-VDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNR 6218
+ C++ G+E +G C+CK N +GP CS C P F + CL CFC TQ+C S YR DVV S V+ NT LVNR
Sbjct: 1136 ERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSSSYYR-------------DVVTS------VFAPGNTQGYALVNR 1220 HSP 5 Score: 58.5362 bits (140), Expect = 1.470e-7 Identity = 47/203 (23.15%), Postives = 75/203 (36.95%), Query Frame = -2
Query: 6226 RPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADER-DCKKTRSI 6807
R +L VL+D+ +LIR Y++ +++ + N + + +E+C CP Y GLSC+ C GY + + C + EC+ + G C C G C +C G+ DC C C S C + G G C C++C ER C+ T ++
Sbjct: 1451 REHLLMVLADLDDILIRASYHTEMRSSSISGVSMEIAVPNYTGLAQAL---EVEQCRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEL--CECNGHSDSCHPETGICTNCLHNTMGELCEQCATGYFGDASAGTPEDCQPCACPHTDPGNQFSPTC--ESLGSGDYQCTACQPGYTGQYCERCAPGYTGNPQERIKCRPTNAL 1646
BLAST of SMED30010499 vs. Ensembl Cavefish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 211.075 bits (536), Expect = 1.477e-63 Identity = 172/627 (27.43%), Postives = 269/627 (42.90%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTV-HQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR---AMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ------------------------SPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKV-ILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCKC--TGRERNDPLCDKC 8040
C +Q C+N CI + C+G +DC DG DE + T + CEPN+F C + +C WRCDG+NDC + DE++C ++ + C F C + IP S CDE P+C + DE C P V P +++ + + ++ C+ V VPT I WR NWG +P T+ + G+ LTI ++ G YTCEA++ K A+ + C + FN C+ CFC G T +C S Y +P P TST+ +L+N + ++ V ++ D++S P FL G+ ++SY +L+ + + + P K IL NG+ L + + +P+ NQ +I E ENW T S R ++L L++++ + IR Y + + +D G P+ +E+C CP Y+GLSCE C +G+++ + + C NC CD +G+C C GP+C +C+PG+ DC C C + S+ C D+ GQ C+ TGR C+KC
Sbjct: 196 CRGDQAMCQNGQCISRDYLCDGERDCTDGSDEMKCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCKDNSDEMDCPVRRPGDACAPEQFVCLSDRACIPASYQCDEEPDCADRSDEYGCAPPTVTSPPEDSVTAMRGGT-VTFTCKAVGVPTPIITWRLNWGHIPVSSRITMTSENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLVFAIPDGVLTLNRVPSNCPEGHFNIGGR----CMPCFCFGITKNCQSTGRYRNQISLRFDEEENFKGVNVSYPIRPGAPPPLTSTQ---ILINPEEEEFQLVDLSRRFLDLDSH------------WTLPRQFL-------GNKVDSYGGFLRYKVSYSLQREGSEPDNKADILLMGNGQRLVYRRGSPT-----SPD-IKNQREIKFTE-ENW--QHTSGRSVTREELLMTLANVESIRIRTVYDNRMVSVGLSDVVMDTTSVEFSLQGQ----PKDVEECRCPPGYSGLSCESCSSGFDRVPGGRYLGTCAGCNCHGHASACDPVSGHCLSCQHNTEGPQCDKCRPGYFGDPSRGRHDDCKPCPCPYTETSRRFSDTCFLDVDGQATCDACRPGYTGRR-----CEKC 764 HSP 2 Score: 55.4546 bits (132), Expect = 1.477e-63 Identity = 78/326 (23.93%), Postives = 124/326 (38.04%), Query Frame = -3
Query: 5301 IVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLL-VNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFT--FEYNT-----DLLKDLVFSDVTPFIE--IESKKFGRLWTVLS--FNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGF----VKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6188
+ +G+ C N GP C EC PG F CL+CFC T++C S R Q+ G N QL + N + +Q G ++ + NS VP DI+ + + YGG++ + FE+ + D D+V F+E ++K R+ TV++ F E WR +G+ RE + AL L I + + + D+ + D+ + H+ + VE C CP+G G C+ C +VGY + + G C+ DC
Sbjct: 780 VPNGEPLCLANVAGPLCDECKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTR-------------------DQVRG-----GVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNS-VPSDIYYWVMPESFRGDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQGNGIFLEHSSQTKPLPRVPTVVTVPFRESA--------------WRRA-DGQPCT-REHLLMALADVAVLMIRATYADKMSESSISDIHM-DIAVPHSTGNE---RAMEVEECACPQGYRGPSCQEC-EVGYTRTGS------GLYLGTCEKCDC 1053 HSP 3 Score: 82.8037 bits (203), Expect = 7.705e-27 Identity = 93/385 (24.16%), Postives = 151/385 (39.22%), Query Frame = -2
Query: 6238 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFV-GKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 7356
C+ F+ +A CL+CFC G T C+S+ + + L ++ I E K +++ +S+ V +Y + V P F G + +Y L+ +R +S + P V+LQ G ++ + ++ + P + S W + + R +L L+D+ L+IR Y + + +D I P S GN A +E+C CP Y G SC++C GY + C K C + ECD + G C +C +GP+C C PGF YG ++ C C C + + C D GQ P C+ C P A R+C++
Sbjct: 799 CKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTRDQVRGGVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNSVPSDIY--YWVMPESFR-------GDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQ---GNGIFLEHSSQTKPLPRVPTVVTVPFRESAWRRADG-------QPCTREHLLMALADVAVLMIRATYADKMSESSISDIHMD--IAVPHSTGNE-RAMEVEECACPQGYRGPSCQECEVGYTRTGSGLYLGTCEKCDCHGHASECDSETGACLQCLHNTAGPRCDSCLPGF---YGNPVTGGIHACRPCPCL----GSAPAKSCYLDSDGQ--------------PTCNNC---PPGYAGRRNCER 1137 HSP 4 Score: 60.4622 bits (145), Expect = 7.705e-27 Identity = 37/104 (35.58%), Postives = 47/104 (45.19%), Query Frame = -3
Query: 5922 KNCNSLGTENI-VDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNR 6218
+ C++ G+E +G C+CK N +GP CS C P F + CL CFC TQ+C S YR DVV S V+ NT LVNR
Sbjct: 1136 ERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSSSYYR-------------DVVTS------VFAPGNTQGYALVNR 1220 HSP 5 Score: 60.077 bits (144), Expect = 5.511e-8 Identity = 95/478 (19.87%), Postives = 155/478 (32.43%), Query Frame = -2
Query: 6226 TINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPM--------FNNAAMFVSMCLKCFCSGQTDDCSSA----------------MGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYA---------------------KSIYDVNS--------KSYKFVGKMYQDFVVDPN--VFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADER-DCKKTRSI 7467
T NN P +AG CE + + K E P F+ + CL CFC G T C+S+ GY +T+ T G + + + S+ K D N Y++ +Y+DF + P+ +F G + SY LK I S+ P + +Q + D + +G + +E+++ R +L VL+D+ +LIR Y++ +++ + N + + +E+C CP Y GLSC+ C GY + + C + EC+ + G C C G C +C G+ DC C C S C + G G C C++C ER C+ T ++
Sbjct: 1121 TCNNCPPGYAGRRNCERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSSSYYRDVVTSVFAPGNTQGYALVNRQRTNRITSGFSVEVSADGTTQTSFTNFDRLGQEPHYWQLPNSYQGDKVQSDTNICNVFFFFFSQYRYA-LVYKDFSLHPDDVLFWQLPPKFRGDKVGSYGGKLKYTISYVPGSRG-APIDDIDVQIIGNDITLVARQD-----WRRGQGTRESRSFEIVFREDYWKRPD-GMPTTREHLLMVLADLDDILIRASYHTEMRSSSISGVSMEIAVPNYTGLAQAL---EVEQCRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEL--CECNGHSDSCHPETGICTNCLHNTMGELCEQCATGYFGDASAGTPEDCQPCACPHTDPGNQFSPTC--ESLGSGDYQCTACQPGYTGQYCERCAPGYTGNPQERIKCRPTNAL 1583
BLAST of SMED30010499 vs. Ensembl Cavefish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 209.92 bits (533), Expect = 3.329e-63 Identity = 172/634 (27.13%), Postives = 271/634 (42.74%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTV-HQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR----------AMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ------------------------SPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKV-ILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCKC--TGRERNDPLCDKC 8040
C +Q C+N CI + C+G +DC DG DE + T + CEPN+F C + +C WRCDG+NDC + DE++C ++ + C F C + IP S CDE P+C + DE C P V P +++ + + ++ C+ V VPT I WR NWG +P T+ + G+ LTI ++ G YTCEA++ K + ++ F + C + FN C+ CFC G T +C S Y +P P TST+ +L+N + ++ V ++ D++S P FL G+ ++SY +L+ + + + P K IL NG+ L + + +P+ NQ +I E ENW T S R ++L L++++ + IR Y + + +D G P+ +E+C CP Y+GLSCE C +G+++ + + C NC CD +G+C C GP+C +C+PG+ DC C C + S+ C D+ GQ C+ TGR C+KC
Sbjct: 191 CRGDQAMCQNGQCISRDYLCDGERDCTDGSDEMKCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCKDNSDEMDCPVRRPGDACAPEQFVCLSDRACIPASYQCDEEPDCADRSDEYGCAPPTVTSPPEDSVTAMRGGT-VTFTCKAVGVPTPIITWRLNWGHIPVSSRITMTSENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLVFAIPDGVLTLNRNAGPFFFAHCNCPEGHFNIGGR----CMPCFCFGITKNCQSTGRYRNQISLRFDEEENFKGVNVSYPIRPGAPPPLTSTQ---ILINPEEEEFQLVDLSRRFLDLDS------------HWTLPRQFL-------GNKVDSYGGFLRYKVSYSLQREGSEPDNKADILLMGNGQRLVYRRGSPT-----SPD-IKNQREIKFTE-ENW--QHTSGRSVTREELLMTLANVESIRIRTVYDNRMVSVGLSDVVMDTTSVEFSLQGQ----PKDVEECRCPPGYSGLSCESCSSGFDRVPGGRYLGTCAGCNCHGHASACDPVSGHCLSCQHNTEGPQCDKCRPGYFGDPSRGRHDDCKPCPCPYTETSRRFSDTCFLDVDGQATCDACRPGYTGRR-----CEKC 766 HSP 2 Score: 55.4546 bits (132), Expect = 3.329e-63 Identity = 78/326 (23.93%), Postives = 124/326 (38.04%), Query Frame = -3
Query: 5301 IVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLL-VNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFT--FEYNT-----DLLKDLVFSDVTPFIE--IESKKFGRLWTVLS--FNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGF----VKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6188
+ +G+ C N GP C EC PG F CL+CFC T++C S R Q+ G N QL + N + +Q G ++ + NS VP DI+ + + YGG++ + FE+ + D D+V F+E ++K R+ TV++ F E WR +G+ RE + AL L I + + + D+ + D+ + H+ + VE C CP+G G C+ C +VGY + + G C+ DC
Sbjct: 782 VPNGEPLCLANVAGPLCDECKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTR-------------------DQVRG-----GVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNS-VPSDIYYWVMPESFRGDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQGNGIFLEHSSQTKPLPRVPTVVTVPFRESA--------------WRRA-DGQPCT-REHLLMALADVAVLMIRATYADKMSESSISDIHM-DIAVPHSTGNE---RAMEVEECACPQGYRGPSCQEC-EVGYTRTGS------GLYLGTCEKCDC 1055 HSP 3 Score: 82.8037 bits (203), Expect = 1.781e-29 Identity = 93/385 (24.16%), Postives = 151/385 (39.22%), Query Frame = -2
Query: 6238 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFV-GKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 7356
C+ F+ +A CL+CFC G T C+S+ + + L ++ I E K +++ +S+ V +Y + V P F G + +Y L+ +R +S + P V+LQ G ++ + ++ + P + S W + + R +L L+D+ L+IR Y + + +D I P S GN A +E+C CP Y G SC++C GY + C K C + ECD + G C +C +GP+C C PGF YG ++ C C C + + C D GQ P C+ C P A R+C++
Sbjct: 801 CKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTRDQVRGGVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNSVPSDIY--YWVMPESF-------RGDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQ---GNGIFLEHSSQTKPLPRVPTVVTVPFRESAWRRADG-------QPCTREHLLMALADVAVLMIRATYADKMSESSISDIHMD--IAVPHSTGNE-RAMEVEECACPQGYRGPSCQECEVGYTRTGSGLYLGTCEKCDCHGHASECDSETGACLQCLHNTAGPRCDSCLPGF---YGNPVTGGIHACRPCPCL----GSAPAKSCYLDSDGQ--------------PTCNNC---PPGYAGRRNCER 1139 HSP 4 Score: 69.3218 bits (168), Expect = 1.781e-29 Identity = 75/308 (24.35%), Postives = 121/308 (39.29%), Query Frame = -3
Query: 5385 KNCNSLGTENI-VDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVK----------------RNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNE--------KTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6218
+ C++ G+E +G C+CK N +GP CS C P F + CL CFC TQ+C S YR DVV S V+ NT LVNR + N +++G +L NS D + YGGK+ +T Y + S P +I+ + G T+++ + ++++ E +F ED W+ + + RE + L + I + ++ + +++E A L + VE C CP G G+ C+ C
Sbjct: 1138 ERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSSSYYR-------------DVVTS------VFAPGNTQGYALVNRQRTNRITSGFSVEVSADGTTQTSFTNFDRLGQEPHYWQLPNSYQGDKVGS----YGGKLKYTISY-------VPGSRGAPIDDIDVQIIGNDITLVARQDWRRGQGTRESRSFEIVFRED---YWK--RPDGMPTTREHLLMVLADLDDILIRASYHTE-MRSSSISGVSMEIAVPNYTGLAQALEVEQCRCPPGYQGLSCQDC 1409 HSP 5 Score: 62.003 bits (149), Expect = 1.413e-8 Identity = 81/378 (21.43%), Postives = 135/378 (35.71%), Query Frame = -2
Query: 6403 TINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPM--------FNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ----SPNPKTSTRTKGLLMNFKSQKILE-VSYAKSIYDVNSKSYKFVGKMYQD--FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7467
T NN P +AG CE + + K E P F+ + CL CFC G T C+S+ YY+ S +T+ L+ ++ +I S S S+ ++ Q+ + PN + G + SY LK I S+ P + +Q + D + +G + +E+++ R +L VL+D+ +LIR Y++ +++ + N + + +E+C CP Y GLSC+ C GY + + C + EC+ + G C C G C +C G+ DC C C
Sbjct: 1123 TCNNCPPGYAGRRNCERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSS-SYYRDVVTSVFAPGNTQGYALVNRQRTNRITSGFSVEVSADGTTQTSFTNFDRLGQEPHYWQLPNSY-------QGDKVGSYGGKLKYTISYVPGSRG-APIDDIDVQIIGNDITLVARQD-----WRRGQGTRESRSFEIVFREDYWKRPD-GMPTTREHLLMVLADLDDILIRASYHTEMRSSSISGVSMEIAVPNYTGLAQAL---EVEQCRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEL--CECNGHSDSCHPETGICTNCLHNTMGELCEQCATGYFGDASAGTPEDCQPCAC 1480
BLAST of SMED30010499 vs. Ensembl Cavefish
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:ZFIN;Acc:ZDB-GENE-080807-4]) HSP 1 Score: 209.92 bits (533), Expect = 5.081e-63 Identity = 172/634 (27.13%), Postives = 271/634 (42.74%), Query Frame = -2
Query: 6283 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTV-HQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKR----------AMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ------------------------SPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKV-ILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPR-IEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCKC--TGRERNDPLCDKC 8040
C +Q C+N CI + C+G +DC DG DE + T + CEPN+F C + +C WRCDG+NDC + DE++C ++ + C F C + IP S CDE P+C + DE C P V P +++ + + ++ C+ V VPT I WR NWG +P T+ + G+ LTI ++ G YTCEA++ K + ++ F + C + FN C+ CFC G T +C S Y +P P TST+ +L+N + ++ V ++ D++S P FL G+ ++SY +L+ + + + P K IL NG+ L + + +P+ NQ +I E ENW T S R ++L L++++ + IR Y + + +D G P+ +E+C CP Y+GLSCE C +G+++ + + C NC CD +G+C C GP+C +C+PG+ DC C C + S+ C D+ GQ C+ TGR C+KC
Sbjct: 191 CRGDQAMCQNGQCISRDYLCDGERDCTDGSDEMKCGTPSPCEPNEFKCK-------NGRCALKLWRCDGDNDCKDNSDEMDCPVRRPGDACAPEQFVCLSDRACIPASYQCDEEPDCADRSDEYGCAPPTVTSPPEDSVTAMRGGT-VTFTCKAVGVPTPIITWRLNWGHIPVSSRITMTSENGQ------GTLTIRDVKEGDQGAYTCEAINAKGLVFAIPDGVLTLNRNAGPFFFAHCNCPEGHFNIGGR----CMPCFCFGITKNCQSTGRYRNQISLRFDEEENFKGVNVSYPIRPGAPPPLTSTQ---ILINPEEEEFQLVDLSRRFLDLDS------------HWTLPRQFL-------GNKVDSYGGFLRYKVSYSLQREGSEPDNKADILLMGNGQRLVYRRGSPT-----SPD-IKNQREIKFTE-ENW--QHTSGRSVTREELLMTLANVESIRIRTVYDNRMVSVGLSDVVMDTTSVEFSLQGQ----PKDVEECRCPPGYSGLSCESCSSGFDRVPGGRYLGTCAGCNCHGHASACDPVSGHCLSCQHNTEGPQCDKCRPGYFGDPSRGRHDDCKPCPCPYTETSRRFSDTCFLDVDGQATCDACRPGYTGRR-----CEKC 766 HSP 2 Score: 55.0694 bits (131), Expect = 5.081e-63 Identity = 78/326 (23.93%), Postives = 124/326 (38.04%), Query Frame = -3
Query: 5301 IVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLL-VNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFT--FEYNT-----DLLKDLVFSDVTPFIE--IESKKFGRLWTVLS--FNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGF----VKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6188
+ +G+ C N GP C EC PG F CL+CFC T++C S R Q+ G N QL + N + +Q G ++ + NS VP DI+ + + YGG++ + FE+ + D D+V F+E ++K R+ TV++ F E WR +G+ RE + AL L I + + + D+ + D+ + H+ + VE C CP+G G C+ C +VGY + + G C+ DC
Sbjct: 782 VPNGEPLCLANVAGPLCDECKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTR-------------------DQVRG-----GVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNS-VPSDIYYWVMPESFRGDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQGNGIFLEHSSQTKPLPRVPTVVTVPFRESA--------------WRRA-DGQPCT-REHLLMALADVAVLMIRATYADKMSESSISDIHM-DIAVPHSTGNE---RAMEVEECACPQGYRGPSCQEC-EVGYTRTGS------GLYLGTCEKCDC 1055 HSP 3 Score: 83.1889 bits (204), Expect = 2.338e-29 Identity = 93/385 (24.16%), Postives = 151/385 (39.22%), Query Frame = -2
Query: 6238 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFV-GKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDI--PGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 7356
C+ F+ +A CL+CFC G T C+S+ + + L ++ I E K +++ +S+ V +Y + V P F G + +Y L+ +R +S + P V+LQ G ++ + ++ + P + S W + + R +L L+D+ L+IR Y + + +D I P S GN A +E+C CP Y G SC++C GY + C K C + ECD + G C +C +GP+C C PGF YG ++ C C C + + C D GQ P C+ C P A R+C++
Sbjct: 801 CKPGSFHLSADNPDGCLQCFCMGVTKQCASSTWTRDQVRGGVNGQLFSLANGANTRTITEGISQKGSAEISYRSFNSVPSDIY--YWVMPESFR-------GDKVTAYGGELRYKVRFEHQSFSLVIDGQPDVVLQ---GNGIFLEHSSQTKPLPRVPTVVTVPFRESAWRRADG-------QPCTREHLLMALADVAVLMIRATYADKMSESSISDIHMD--IAVPHSTGNE-RAMEVEECACPQGYRGPSCQECEVGYTRTGSGLYLGTCEKCDCHGHASECDSETGACLQCLHNTAGPRCDSCLPGF---YGNPVTGGIHACRPCPCL----GSAPAKSCYLDSDGQ--------------PTCNNC---PPGYAGRRNCER 1139 HSP 4 Score: 68.9366 bits (167), Expect = 2.338e-29 Identity = 75/308 (24.35%), Postives = 121/308 (39.29%), Query Frame = -3
Query: 5385 KNCNSLGTENI-VDGKCKCKENYKGPNCSECSPGFFSF----QKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVK----------------RNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNE--------KTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6218
+ C++ G+E +G C+CK N +GP CS C P F + CL CFC TQ+C S YR DVV S V+ NT LVNR + N +++G +L NS D + YGGK+ +T Y + S P +I+ + G T+++ + ++++ E +F ED W+ + + RE + L + I + ++ + +++E A L + VE C CP G G+ C+ C
Sbjct: 1138 ERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSSSYYR-------------DVVTS------VFAPGNTQGYALVNRQRTNRITSGFSVEVSADGTTQTSFTNFDRLGQEPHYWQLPNSYQGDKVGS----YGGKLKYTISY-------VPGSRGAPIDDIDVQIIGNDITLVARQDWRRGQGTRESRSFEIVFRED---YWK--RPDGMPTTREHLLMVLADLDDILIRASYHTE-MRSSSISGVSMEIAVPNYTGLAQALEVEQCRCPPGYQGLSCQDC 1409 HSP 5 Score: 62.3882 bits (150), Expect = 1.246e-8 Identity = 81/378 (21.43%), Postives = 135/378 (35.71%), Query Frame = -2
Query: 6403 TINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPM--------FNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQ----SPNPKTSTRTKGLLMNFKSQKILE-VSYAKSIYDVNSKSYKFVGKMYQD--FVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7467
T NN P +AG CE + + K E P F+ + CL CFC G T C+S+ YY+ S +T+ L+ ++ +I S S S+ ++ Q+ + PN + G + SY LK I S+ P + +Q + D + +G + +E+++ R +L VL+D+ +LIR Y++ +++ + N + + +E+C CP Y GLSC+ C GY + + C + EC+ + G C C G C +C G+ DC C C
Sbjct: 1123 TCNNCPPGYAGRRNCERCDARGSEGCSANGVCRCKMNVEGPTCSNCKPRTFHLSPANKDGCLICFCMGITQQCTSS-SYYRDVVTSVFAPGNTQGYALVNRQRTNRITSGFSVEVSADGTTQTSFTNFDRLGQEPHYWQLPNSY-------QGDKVGSYGGKLKYTISYVPGSRG-APIDDIDVQIIGNDITLVARQD-----WRRGQGTRESRSFEIVFREDYWKRPD-GMPTTREHLLMVLADLDDILIRASYHTEMRSSSISGVSMEIAVPNYTGLAQAL---EVEQCRCPPGYQGLSCQDCAPGYTRTGGGLYLGHCEL--CECNGHSDSCHPETGICTNCLHNTMGELCEQCATGYFGDASAGTPEDCQPCAC 1480
BLAST of SMED30010499 vs. Ensembl Cavefish
Match: lama1 (laminin, alpha 1 [Source:ZFIN;Acc:ZDB-GENE-060118-1]) HSP 1 Score: 89.7373 bits (221), Expect = 5.383e-17 Identity = 72/267 (26.97%), Postives = 124/267 (46.44%), Query Frame = -2
Query: 6283 SWLESYSYYLKINIRLFGESKDD-----IPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQ----NQCVK-KCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7053
S L +Y +L ++ + S D+ +P ++ NG+SL S ++ NP + + + L ++F+D E R ++++VL+D+ L IR ++ G ++V + +P S + AP +E+C CP Y+G SCE C++G+ + C++ +C + ECD NG C C+ +GP C +C PG+ E + DC C C L + S C L G G I+C + C++C
Sbjct: 514 SLLTAYGSFLNYSV-AYDASVDNNVDKTLPSHFDVVIQGNGRSLRQL---QSRRVFLNP---HMEQHVVLEFLPHFFVDMHMERRVQRDELMSVLADVASLRIRAHLNTSVDGVLRL-SSVSLAVADPISR-SAAPAPDVEQCECPWGYSGTSCEYCISGFYRVGGILFGGNCLQCECNDHATECDINGVCLGCSHNTTGPHCDQCLPGYYGDPTEGTAEDCQRCACPLTLASNNFSPTC--SLQGPGEIMCDQCEQGYTGTKCERC 769 HSP 2 Score: 61.2326 bits (147), Expect = 2.143e-11 Identity = 85/391 (21.74%), Postives = 151/391 (38.62%), Query Frame = -2
Query: 6283 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSP------NPKTSTRTKGLL---MNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPG--PKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLK----ISLWEKENWFIDSTCETSAMRPDILAVLSDIKY-LLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQ-------------CVK-KCPINCVECDKN-GNCNRCTGQRSGPKCLECKPGFVRLYGEKLS--SDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 7356
C+ F S C CFC G + C G + P + + + KG L + + + +L+V+ +S + Y + + +Q GS L SY L ++ + P+V+++ + K L VIY + N ++ + L E + + ++ E + D +++LS++ +L+ + Y N ++D + ++ A +E C CP Y GLSC++C GY + ++ CV +C + + CD + G C C +G C C PG+ YG+ SDC+ C C L++ S C+ L G G C C++C
Sbjct: 1069 CKTGTFGLWGQNPSGCSPCFCFGVSTVCEELGGLVRVPVSAERLHVVSQSDLKGTLEGVYHTEGEMLLDVTQLQSASSLTGPYYWRLPQRFQ-----------------GSKLLSYGGDLSYSVAFAALDGSGLSNHEPQVLIRGGHLKKL---------VIYMDMPAPENSIRTTQRVPLTEHKWKYFNAVSEKAVSHADFMSILSNLHINILVLMANYLLFLYMLISNISMDTAVEADEAPEGTPEARLVEACECPPGYAGLSCQECAPGYYRQHVSELNMKGMKRPMMQPCVPCQCNNHSLACDLDTGKCLGCQHNTTGENCNVCAPGY---YGQVKGSISDCSLCAC--PLHSNSFSPTCV--LEGVGDFRCDACKPGYEGRYCERC 1426 HSP 3 Score: 30.0314 bits (66), Expect = 2.143e-11 Identity = 11/31 (35.48%), Postives = 15/31 (48.39%), Query Frame = -3
Query: 6087 GKCKCKENYKGPNCSECSPGFFSFQKKCLKC 6179
GKC+CK +G C +C +CL C
Sbjct: 1461 GKCECKAGVRGHLCDQCEDRHVLSNDQCLSC 1491 HSP 4 Score: 47.3654 bits (111), Expect = 3.071e-9 Identity = 43/129 (33.33%), Postives = 59/129 (45.74%), Query Frame = -2
Query: 6283 CPTPYTGLSCEKCLAGYEKN----DQNQCVKKC-----PINCVECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSS-DCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKC 6636
C YTG CE+C GY N Q V +C P CD G C +C G SG C C+ G+ +G+ +++ DC C C++ N VS++C +TGQ C C D CD+C
Sbjct: 757 CEQGYTGTKCERCANGYYGNPTVAGQKCSVCECNGNVDPSEPGHCDAVTGECLKCVGHTSGRHCERCEDGY---FGDAVTAKDCQACGCHA---NGSVSSVC-NIITGQ----CDCRANVVGDK-CDRC 873 HSP 5 Score: 36.1946 bits (82), Expect = 3.071e-9 Identity = 14/42 (33.33%), Postives = 20/42 (47.62%), Query Frame = -3
Query: 6060 DGKCKCKENYKGPNCSECSPGFFSFQK-KCLKCFCSNKTQEC 6182
DG+C+C G C C GF++F C C C++ C
Sbjct: 904 DGQCQCIVGVSGAKCDRCQQGFYNFTDGGCTPCDCAHTHGNC 945 HSP 6 Score: 41.2022 bits (95), Expect = 7.625e-6 Identity = 21/59 (35.59%), Postives = 30/59 (50.85%), Query Frame = -3
Query: 6051 CNSLGTENIVDGK-CKCKENYKGPNCSECSPGFFSFQ----KKCLKCFCSNKTQECKES 6212
C+ G+ NI + C CK N G NC C GF++ Q + C +CFC + C+ S
Sbjct: 451 CSLKGSHNIDPCRECICKVNVMGANCDLCKQGFYNLQASNPEGCTECFCFGVSDVCEGS 509 HSP 7 Score: 30.8018 bits (68), Expect = 7.625e-6 Identity = 27/100 (27.00%), Postives = 43/100 (43.00%), Query Frame = -2
Query: 6283 NGNCNRCTGQRSGPKCLECKPGFVRLYGEK--LSSDCTECNCYSQLNNTLVSNICMRDLT----GQGAIVCKCTGRE--------------RNDPLCDKC 6522
G C CT +G C C G+ R + + C ECNC + + VS++C++D G+G + +C +E R+ PLC +C
Sbjct: 353 GGVCINCTQNTAGVNCETCADGYYRPHKMSPYATEPCLECNCDMRGS---VSHVCLKDDKHANPGKGLLPGQCVCKEGFAGEKCDRCAFGYRDFPLCTRC 449
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Match: lrp1ab (low density lipoprotein receptor-related protein 1Ab [Source:ZFIN;Acc:ZDB-GENE-030131-7126]) HSP 1 Score: 77.7962 bits (190), Expect = 5.827e-14 Identity = 45/124 (36.29%), Postives = 64/124 (51.61%), Query Frame = -2
Query: 7678 DF-CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8046
DF C Q C+N IC + C+G DC DG DE C P+QF C T +C+ +RC+G+++CG+G DE +C ++ TC + F C IPR CD + +C +G DE
Sbjct: 3340 DFKCRPGQFQCKNGICTNPAFICDGDNDCGDGSDEANCDVHVCLPSQFKCRTT------HRCIPGIFRCNGQDNCGDGEDEKDCPEV---TCAPNQFQCSSTKRCIPRVWVCDRDNDCGDGSDE 3454 HSP 2 Score: 72.0182 bits (175), Expect = 2.659e-12 Identity = 46/127 (36.22%), Postives = 62/127 (48.82%), Query Frame = -2
Query: 7669 FNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NCIKL---DKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8034
Q AC N CI+ N C+ DC D DE I C +QF C++ +C+ ++W CDG+NDCG+ DE NC KL C G F C + IP CD + +C++ DEK C
Sbjct: 952 LTQFACNNGRCINMNWRCDADNDCADASDE-IGCNHSCSISQFKCNS-------GRCIPSHWTCDGDNDCGDFSDETNPNCTKLATRPPGGCHGDEFQCKADGQCIPLRWRCDGDTDCIDTSDEKEC 1070 HSP 3 Score: 72.0182 bits (175), Expect = 2.843e-12 Identity = 46/135 (34.07%), Postives = 65/135 (48.15%), Query Frame = -2
Query: 7636 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQE 8040
C Q C+N+ C+ C+ DC D DEE T C ++FSC + +C+A WRCDG++DC +G DE +C C F C N IP+ CD + +C++G DE NC P+ E
Sbjct: 3505 CEPYQFRCKNNRCVPGRWQCDYDNDCGDNSDEEGCTPRPCSESEFSC-------ANGRCIAGRWRCDGDHDCSDGSDEKDC----SPRCDHDQFQC-RNGHCIPQRWRCDADIDCIDGSDEDNCAAIVRTCPHHE 3627 HSP 4 Score: 71.2478 bits (173), Expect = 5.241e-12 Identity = 53/137 (38.69%), Postives = 68/137 (49.64%), Query Frame = -2
Query: 7639 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVV-IPNQ 8040
C+ ++ C+N CI C+ DC D DE + C +C P QF C + C + CDG+NDCG+G DE NC D + CL S F C IP C+ NC +GEDEK+C PEV PNQ
Sbjct: 3303 CTASRFVCKNDKCIPFWWKCDTEDDCGDRSDEPAD-CPDFKCRPGQFQCK-------NGICTNPAFICDGDNDCGDGSDEANC---DVHVCLPSQFKCRTTHRCIPGIFRCNGQDNCGDGEDEKDC--PEVTCAPNQ 3426 HSP 5 Score: 67.3958 bits (163), Expect = 7.652e-11 Identity = 46/131 (35.11%), Postives = 60/131 (45.80%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ----CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C +Q CRN CI + C+ DC DG DE+ CA C ++F C+ KP W+CDGE+DCG+ DE + L G F C +N CD NC +G DE+NC P
Sbjct: 3582 CDHDQFQCRNGHCIPQRWRCDADIDCIDGSDED--NCAAIVRTCPHHEFQCNNTLCKPLS-------WKCDGEDDCGDNSDETPEMCLKFQCPPGRQFRCRNNRVCFWAGRQCDGVDNCGDGSDEENCEKP 3703 HSP 6 Score: 66.6254 bits (161), Expect = 1.330e-10 Identity = 63/206 (30.58%), Postives = 87/206 (42.23%), Query Frame = -2
Query: 7660 TVIWRREDKKSFQI---DVESRVGGETQALLIVNTATKEM-----NGTVYECVHNNLLIKATLIVK---DF-----CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE-KNCRHP 8220
T + R E F+I D E + G T A + N + G C + LL + K D+ C + AC+N CI E C+G DC D DE C Q C ++F C + +C+ W CDG+NDCGN DE N TC + F+C IP S CD + +C + DE +C +P
Sbjct: 752 TTVLRNERPPIFEIRTYDREQQKSG-TNACHVNNGGCSILCLAVPEGRACACADDQLLDADNITCKPNPDYTPPPQCHPGEFACKNGRCIQERWRCDGDNDCLDNSDEAPEICHQHTCPSDRFKCQ-------NNRCIPLRWLCDGDNDCGNSEDESN-TTCAARTCPPNQFSCASG-HCIPMSWKCDLDDDCGDRSDEPPSCAYP 947 HSP 7 Score: 61.2326 bits (147), Expect = 5.263e-9 Identity = 41/117 (35.04%), Postives = 55/117 (47.01%), Query Frame = -2
Query: 7672 SICIDENKFCNGIKDCEDGFDEE----INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKN 8010
S+C+ C+G DC D DE+ + C P+ F+C P C+ W+CD E DC +G DELNC L C HF C DN I + CD +C + DEK+
Sbjct: 2641 SLCVLPAWICDGANDCGDYADEKNCKKKSGSPGCSPDYFNC-------PSGPCIPTAWKCDREPDCDDGADELNCDML----CAVDHFEC-DNGKCITKRWMCDGEDDCGDRSDEKD 2745 HSP 8 Score: 60.4622 bits (145), Expect = 8.848e-9 Identity = 39/132 (29.55%), Postives = 60/132 (45.45%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--------CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS +Q C + CI + C+G DC D DE C + C ++F C G +C+ WRCDG+ DC + DE C + + F+C D I ++ +CD + +C + DE+ C
Sbjct: 988 CSISQFKCNSGRCIPSHWTCDGDNDCGDFSDETNPNCTKLATRPPGGCHGDEFQCKADG------QCIPLRWRCDGDTDCIDTSDEKECDGVVRTCDPTLQFSCRDTGRCISKTWFCDGDNDCEDNSDEEGC 1113 HSP 9 Score: 59.6918 bits (143), Expect = 1.475e-8 Identity = 44/129 (34.11%), Postives = 62/129 (48.06%), Query Frame = -2
Query: 7663 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLG-SHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH 8040
C ++ C+N+ CI C+G DC + DE TCA C PNQFSC + C+ W+CD ++DCG+ DE TC + FAC+ N I + CD + +C + DE C H
Sbjct: 868 CPSDRFKCQNNRCIPLRWLCDGDNDCGNSEDESNTTCAARTCPPNQFSCAS-------GHCIPMSWKCDLDDDCGDRSDEPPSCAYP--TCFPLTQFACN-NGRCINMNWRCDADNDCADASDEIGCNH 986 HSP 10 Score: 58.151 bits (139), Expect = 4.495e-8 Identity = 41/110 (37.27%), Postives = 53/110 (48.18%), Query Frame = -2
Query: 7717 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAW 8040
C +Q CR + CI CNG +C DG DE+ C PNQF C + ++C+ W CD +NDCG+G DE NC ++ TC F C IP S W
Sbjct: 3382 CLPSQFKCRTTHRCIPGIFRCNGQDNCGDGEDEKDCPEVTCAPNQFQCSST------KRCIPRVWVCDRDNDCGDGSDEPANCTQM---TCGQEEFRCKGTGRCIP-SRW 3481 HSP 11 Score: 54.6842 bits (130), Expect = 5.018e-7 Identity = 46/160 (28.75%), Postives = 60/160 (37.50%), Query Frame = -2
Query: 7669 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSC-----------------------------------HTVGGKPPH-------QKCVANYWRCDGENDCGNGYDELNCIKLDKNT-CLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8019
C N C++ K CNG+ DC D DE + C ++F C H +G + CV W CDG NDCG+ DE NC K + C +F C IP + CD P+C +G DE NC
Sbjct: 2550 CLNGRCMETLKLCNGVNDCGDNSDEIACNNSTCGADEFRCGDGSCIANVTRCNQVVDCDDISDEIGCGPMNCTHYHRLGVQASGYVSCNSTSLCVLPAWICDGANDCGDYADEKNCKKKSGSPGCSPDYFNCPSG-PCIPTAWKCDREPDCDDGADELNC 2708
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Match: lrp2a (low density lipoprotein receptor-related protein 2a [Source:ZFIN;Acc:ZDB-GENE-050119-2]) HSP 1 Score: 74.3294 bits (181), Expect = 6.143e-13 Identity = 44/131 (33.59%), Postives = 67/131 (51.15%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE-INTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C Q C + CI + C+G DC DG DE+ + C C P++F+C PP ++C+ + CDG+ DC + DE NC + +C FACD+ + + R CD +C +G DE+ C +P
Sbjct: 2868 CPGTQFTCDSGRCIPGSWICDGDNDCGDGSDEDGRHACGARTCGPHEFTC--ADASPPRRRCIQRRFVCDGDADCSDALDEHQNCT---RRSCTAGEFACDNGLCVHSRYR-CDRRNDCGDGSDERGCTYP 2992 HSP 2 Score: 71.633 bits (174), Expect = 4.046e-12 Identity = 45/127 (35.43%), Postives = 57/127 (44.88%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C Q C + CID C+G DC DG DE C QFSC + +C+A Y+RCDG DC + DE+ C C F C + IP CD +P+C +G DE HP
Sbjct: 1111 CHPMQFQCPDHRCIDNAYVCDGDSDCVDGADER-GCVFNCTSYQFSCSS------GDQCIATYYRCDGAFDCRDRSDEVGCPTRAPGLCHNDEFQCQSDGLCIPAGWECDGHPDCDDGSDE----HP 1226 HSP 3 Score: 64.3142 bits (155), Expect = 6.651e-10 Identity = 40/125 (32.00%), Postives = 58/125 (46.40%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C+ + AC N +C+ C+ DC DG DE T C NQF+C + +C+ + CDG+NDCG+G DE + TC F C + C++ +C +G DEK C
Sbjct: 2955 CTAGEFACDNGLCVHSRYRCDRRNDCGDGSDERGCTYPTCRQNQFTCE-------NGRCIPRIFACDGDNDCGDGSDEDTHLCHQPSPTCAPGFFRCGAG-ECVAIHLVCNQANDCADGSDEKGC 3071 HSP 4 Score: 63.1586 bits (152), Expect = 1.300e-9 Identity = 54/173 (31.21%), Postives = 68/173 (39.31%), Query Frame = -2
Query: 7669 TLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKP-----------------PHQKCVANYWRCD---------------------GENDCGNGYDELNCIKLDKNTC--LGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8061
TL C+ NQ C N CI C+G DC+DG DE+ TCA C P QF C+ P PH C+ RCD G +DC + DE C ++ TC LG + DN IP CD +C +G DE+NC
Sbjct: 3552 TLCFNHRCNSNQWQCMNKRCIPAAWLCDGGPDCQDGSDEDSATCAARTCAPGQFRCNNGRCIPRSWVCDVDDDCGDGSDEPHDTCMGPSHRCDSHTEFACRTNYRCIPTWAVCNGMDDCRDNSDEQGCAEI---TCDPLGDYRC--DNHRCIPLRWRCDRYDDCGDGSDERNC 3719 HSP 5 Score: 63.1586 bits (152), Expect = 1.563e-9 Identity = 43/140 (30.71%), Postives = 59/140 (42.14%), Query Frame = -2
Query: 7669 ACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHT-----VGGKPPH---------------QKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8022
AC N C+ C+G DC DG DE C P F+C + +P +C+ YW CD +NDCG+G DE C TC F C D+ I + CD + +C++G DE+ C
Sbjct: 1009 ACANGRCVPRLFLCDGYDDCHDGSDERGCGTGNVTCSPYAFTCTNRRCIPIDWRPTRLATTCRADQFTCASGNRCIPRYWICDTDNDCGDGSDERGCDM--SGTCHPMQFQCPDH-RCIDNAYVCDGDSDCVDGADERGC 1145 HSP 6 Score: 61.2326 bits (147), Expect = 5.180e-9 Identity = 43/125 (34.40%), Postives = 58/125 (46.40%), Query Frame = -2
Query: 7669 SFNQRACR-NSICIDENKFCNGIKDCEDGFDEEINTCAQCEP-NQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8037
S + ACR N CI CNG+ DC D DE+ C+P + C + +C+ WRCD +DCG+G DE NC C S F C N IP C+ +C + DE++C
Sbjct: 3645 SHTEFACRTNYRCIPTWAVCNGMDDCRDNSDEQGCAEITCDPLGDYRCD-------NHRCIPLRWRCDRYDDCGDGSDERNCA---PRPCSESEFRC-TNQRCIPGRWVCNHENDCGDNSDERDC 3758 HSP 7 Score: 60.4622 bits (145), Expect = 9.389e-9 Identity = 44/129 (34.11%), Postives = 63/129 (48.84%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNCRHP 8040
C Q C + CI + C+ I DC D DE + A C QF C + +C+A + CDGE DC + DE NC +TC SHF C + +SA+ CD +C + DE++C++P
Sbjct: 1 CDSGQFRCGSGACIPRSWVCDSINDCSDDSDERGCSPATCTTLQFRCRS------DNRCIARAFVCDGEADCADRSDEHQNC---PGSTCSSSHFTCASGHCV--QSAFRCDHFQDCDDRSDEQSCQYP 118 HSP 8 Score: 58.5362 bits (140), Expect = 3.648e-8 Identity = 47/157 (29.94%), Postives = 68/157 (43.31%), Query Frame = -2
Query: 7669 MNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ----CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDD----NVTLIPRSAWCDENPNCLNGEDE-KNC 8112
++G E N+L L + N+ C N CI + C+ +DC D DE ++C+ C QF+C + +C+ W CDG+NDCG+G DE TC F C D I R CD + +C + DE +NC
Sbjct: 2802 LSGGAAEWRRNDLRRPPPLPLHTCFIANEFRCGNGQCIPLSWVCDSEQDCTDNADEP-SSCSDPGRTCPGTQFTCDS-------GRCIPGSWICDGDNDCGDGSDEDGRHACGARTCGPHEFTCADASPPRRRCIQRRFVCDGDADCSDALDEHQNC 2950 HSP 9 Score: 56.6102 bits (135), Expect = 1.209e-7 Identity = 39/142 (27.46%), Postives = 62/142 (43.66%), Query Frame = -2
Query: 7678 VYECVHNNLLIKATLIVKDFCSFNQRACR-NSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8100
++C + + C ++ C+ + +CI C+G DC+DG DE C P +C + + CV WRCDG+NDC +G DE +C S + C ++ + CD N +C +G DE
Sbjct: 1172 AFDCRDRSDEVGCPTRAPGLCHNDEFQCQSDGLCIPAGWECDGHPDCDDGSDEH----PGCPPR--TCAAGYLRCDNGNCVPESWRCDGDNDCRDGTDERDCPTPPYRCQSPSQWLCPNHRACVDLRKVCDGNFDCPDGGDE 1307 HSP 10 Score: 56.225 bits (134), Expect = 1.662e-7 Identity = 51/189 (26.98%), Postives = 74/189 (39.15%), Query Frame = -2
Query: 7663 YECVHNNLLIKATLIVKDFCSFN------QRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTV----------GG----------KPP----------------HQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH 8097
Y C H N ++ ++ C+F + AC N CI ++ CNG+ +C D + C + C+P C T GG +PP + +C+ W CD E DC + DE + TC G+ F CD IP S CD + +C +G DE RH
Sbjct: 2719 YRCDHTNDCGDSS--DEEGCAFRTCDPAAEFACTNGRCIARSQVCNGVNNCRDNATSDEQRCPERTCQPGLLKCLTTNICVLRHYLSGGAAEWRRNDLRRPPPLPLHTCFIANEFRCGNGQCIPLSWVCDSEQDCTDNADEPSSCSDPGRTCPGTQFTCDSG-RCIPGSWICDGDNDCGDGSDEDG-RH 2903 HSP 11 Score: 56.225 bits (134), Expect = 1.916e-7 Identity = 40/122 (32.79%), Postives = 56/122 (45.90%), Query Frame = -2
Query: 7660 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE--KNCRHP 8019
C + CI E C+G DC+D DE + C PN C + + C+ W+CDG+NDCG+G DE LD F CD+N + C+ +C +G DE C+ P
Sbjct: 3770 CGSGHCIPERLKCDGSADCQDFSDESL--CPTRYPNGTWCPPSSFECDNHVCIPPSWKCDGDNDCGDGSDEELHHCLDTPCNPPGRFRCDNNRCIYSHEI-CNAVDDCGDGSDETPDKCKEP 3888 HSP 12 Score: 55.8398 bits (133), Expect = 2.423e-7 Identity = 46/131 (35.11%), Postives = 57/131 (43.51%), Query Frame = -2
Query: 7657 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTC-LGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPE 8040
C +Q C N CI C+ +C DG DE CA CEP F+C + +CV +RCD NDCG+ DE C TC + FAC N I RS C+ NC + R PE
Sbjct: 2661 CGTSQFTCLNGRCIPFRNKCDNRDNCRDGSDELERVCAFHTCEPTDFTCR-------NGRCVRYGYRCDHTNDCGDSSDEEGCA---FRTCDPAAEFAC-TNGRCIARSQVCNGVNNCRDNATSDEQRCPE 2780 HSP 13 Score: 55.4546 bits (132), Expect = 3.221e-7 Identity = 39/126 (30.95%), Postives = 53/126 (42.06%), Query Frame = -2
Query: 7669 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDEE-INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C +Q C + CI C+ DC DG DE + C P QF C P +C+ N + CDG++DC +G DE C+ C F+C I CD +C + DE C
Sbjct: 1070 CRADQFTCASGNRCIPRYWICDTDNDCGDGSDERGCDMSGTCHPMQFQC-------PDHRCIDNAYVCDGDSDCVDGADERGCVF----NCTSYQFSCSSGDQCIATYYRCDGAFDCRDRSDEVGC 1184 HSP 14 Score: 54.299 bits (129), Expect = 6.296e-7 Identity = 42/124 (33.87%), Postives = 54/124 (43.55%), Query Frame = -2
Query: 7675 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNT--CLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK 8040
CS ++ C N CI CN DC D DE C P F C + C+ +CDG DC + DE C N C S F CD++V IP S CD + +C +G DE+
Sbjct: 3724 CSESEFRCTNQRCIPGRWVCNHENDCGDNSDERDCELQTCLPGYFQCGS-------GHCIPERLKCDGSADCQDFSDESLCPTRYPNGTWCPPSSFECDNHV-CIPPSWKCDGDNDCGDGSDEE 3839 HSP 15 Score: 52.373 bits (124), Expect = 2.638e-6 Identity = 38/130 (29.23%), Postives = 58/130 (44.62%), Query Frame = -2
Query: 7669 FCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQC---EPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8043
+C + C N +CI + C+G DC DG DEE++ C P +F C + +C+ ++ C+ +DCG+G DE C + C F C IP +A C+ C + DE C
Sbjct: 3806 WCPPSSFECDNHVCIPPSWKCDGDNDCGDGSDEELHHCLDTPCNPPGRFRCD-------NNRCIYSHEICNAVDDCGDGSDETPDKCKEPTFAPCTEEEFKCASG-RCIPMAAVCNNYNECRDNTDETGC 3927
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000001137.1 (pep scaffold:Pmarinus_7.0:GL476464:95621:179961:-1 gene:ENSPMAG00000001008.1 transcript:ENSPMAT00000001137.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 73.559 bits (179), Expect = 7.868e-13 Identity = 48/135 (35.56%), Postives = 66/135 (48.89%), Query Frame = -2
Query: 7636 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQE 8040
C Q C+N+ CI C+ DC D DEE T C ++FSC + +C+A WRCDG++DC +G DE +C C F C N IP+ CD + +CL+G DE+NC + P E
Sbjct: 876 CEPYQFRCKNNRCIPGRWHCDYDNDCGDNSDEEGCTPRPCSESEFSC-------ANGRCIAGRWRCDGDHDCMDGSDEKDC----SLRCEQDQFQC-QNGHCIPQRWRCDNDADCLDGSDEENCGSAVRLCPKDE 998 HSP 2 Score: 63.929 bits (154), Expect = 8.262e-10 Identity = 45/132 (34.09%), Postives = 62/132 (46.97%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA-------QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 8040
C+ +Q +C+N CI C+G DC D +E +TC QC ++F C G C+ WRCDG+ DC + DE C + G+HF C N I R AW CD + +C + DE C
Sbjct: 150 CADSQFSCKNGHCIPSQWVCDGDNDCGD-VSDETHTCEFPARPLRQCREDEFRCAING------NCIPLRWRCDGDADCMDISDEKECDGQRRTCNPGTHFTCVSNGICI-RKAWVCDGDNDCEDNSDEAGC 273 HSP 3 Score: 57.3806 bits (137), Expect = 6.856e-8 Identity = 41/118 (34.75%), Postives = 56/118 (47.46%), Query Frame = -2
Query: 7660 CIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE-KNCRHP 8004
CI + C+G DC+DG DE C Q C ++F C + +C+ W CDG+NDCG DE N TC F C N IP + CD + +C + DE +C +P
Sbjct: 1 CIQDQWRCDGDNDCQDGSDELPEICQQHTCISDRFKCK-------NNRCIPMRWLCDGDNDCGENEDESN-TTCTVRTCPAEQFTC-GNGKCIPIAWKCDLDNDCSDNSDEPPSCAYP 109 HSP 4 Score: 55.4546 bits (132), Expect = 2.883e-7 Identity = 43/135 (31.85%), Postives = 60/135 (44.44%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLG------SHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C +Q C+N CI + C+ DC DG DEE A C ++F C++ KP WRCDGE+DCG+ DE D + C F C ++ + CD NC + DE++C P
Sbjct: 953 CEQDQFQCQNGHCIPQRWRCDNDADCLDGSDEENCGSAVRLCPKDEFQCNSTLCKP-------FSWRCDGEDDCGDNSDE------DADHCRAFQCPPRRQFRCRNDRVCVSLDRVCDGINNCGDHSDEEHCGEP 1074 HSP 5 Score: 52.7582 bits (125), Expect = 1.682e-6 Identity = 51/149 (34.23%), Postives = 66/149 (44.30%), Query Frame = -2
Query: 7660 ECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA--QCEP-NQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKN-CRHP 8094
+C N T V+ C Q C N CI C+ DC D DE +CA C P QFSC+ + +C+ WRCD +NDC + DE C NTC S F+C N IP CD + +C + DE + C P
Sbjct: 54 DCGENEDESNTTCTVR-TCPAEQFTCGNGKCIPIAWKCDLDNDCSDNSDEPP-SCAYPTCLPLTQFSCN-------NGRCINIRWRCDSDNDCADSSDEAGC----NNTCADSQFSC-KNGHCIPSQWVCDGDNDCGDVSDETHTCEFP 188 HSP 6 Score: 52.373 bits (124), Expect = 2.335e-6 Identity = 33/94 (35.11%), Postives = 48/94 (51.06%), Query Frame = -2
Query: 7585 CVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEV 7866
C+ + WRCDG+NDC +G DEL I ++TC+ F C +N IP CD + +C EDE N P ++ T I+ KC++
Sbjct: 1 CIQDQWRCDGDNDCQDGSDELPEI-CQQHTCISDRFKCKNN-RCIPMRWLCDGDNDCGENEDESNTTCTVRTCPAEQFTCGNGKCIPIAWKCDL 92
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000008925.1 (pep scaffold:Pmarinus_7.0:GL476496:30277:70426:-1 gene:ENSPMAG00000008054.1 transcript:ENSPMAT00000008925.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 69.707 bits (169), Expect = 7.950e-13 Identity = 83/340 (24.41%), Postives = 142/340 (41.76%), Query Frame = -2
Query: 6346 CLKCFCSGQTDDCSS-AMGYYQSPNPK--TSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKI----NIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYS-NQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKC-PINCV----ECDK-NGNC----NRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTG 7311
C CFC G + C S G Q + + T +GL K + +EV + N+ + + +Y + + P +L G+ L +Y+ +L+ +I + + I VI++ NG++L + S + P + + +SL F D R ++ L+++ L IR Y S Q + ++D + +PD+ + A +E+C+CP Y G SCE C+ G+ + D +C P C +CD +G+C + C GP C C+PGF + DC C C + + S C +D G
Sbjct: 386 CSACFCFGLSSSCRSLPWGVTQVVDMRGWRVTDRQGLR---KVKTFVEVD---QVAVRNADVRRTLPALY--YWLAPTSYL-------GNKLTAYAGHLRYSVSYDIPVDSTDSEMISDVDVIIEG-NGQAL----SSGSLGLMLQP---FEEQTLSLRLLPENFFDFRSNAPVSRDALMTALANVTRLQIRASYSSVKQAVYRLSAVSLD--VASPDAAVGSPAALDVEQCHCPHGYAGTSCESCMRGHRRVDGTLHGGRCEPCRCHGHADDCDDLSGDCMLPLSGCRHNTMGPHCELCRPGFYGNATRGTADDCLPCTCPLSIASNNFSPTCHQDPRG 700 HSP 2 Score: 23.8682 bits (50), Expect = 7.950e-13 Identity = 18/71 (25.35%), Postives = 30/71 (42.25%), Query Frame = -3
Query: 6045 RDLSENKNCNSLGTENIVD---GKC-KCKENYKGPNCSECSPGFF---SFQKKCLKCFCSNKTQECKESDQ 6236
R N N + G + D G+C +C+E G +C C+ GF+ + C C C + + D+
Sbjct: 735 RRCECNGNEEAWGGGRVCDARTGQCLRCRERTAGFHCERCADGFYGDATGTGGCQPCQCHPEGATAPQCDR 805
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Match: sorl1 (sortilin-related receptor, L(DLR class) A repeats containing [Source:ZFIN;Acc:ZDB-GENE-050208-22]) HSP 1 Score: 72.0182 bits (175), Expect = 2.024e-12 Identity = 52/148 (35.14%), Postives = 74/148 (50.00%), Query Frame = -2
Query: 7606 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDEEINTCAQ-CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKN-TCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSS 8040
C NQ C+NS CI + C+ DC D DE + Q C+ +FSC + +CV + WRCDG+NDC + DE+NC N +C F C IPR CD + +CL+G DE+ + ++P Q T ++ SS
Sbjct: 660 CGTNQFRCQNSGRCIPMSYKCDQEDDCGDNSDEPADCPHQKCQATEFSCS-------NGRCVRSAWRCDGDNDCHDWSDEVNCSSSQGNISCSADGFQCPSG-PCIPRRWLCDGDKDCLDGADEQ--QQCGGLVPPQPTPGHLNCTSS 797 HSP 2 Score: 69.3218 bits (168), Expect = 1.525e-11 Identity = 45/144 (31.25%), Postives = 68/144 (47.22%), Query Frame = -2
Query: 7633 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGK--PPHQ------KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQET 8040
C+ ++ CR+ +CI + C+G+ DC D + +E N A C S TV + P Q +CV +W+CD +NDCG+ DE C + C F C D + CD +P+C +G DE C VI + +
Sbjct: 794 CTSSEFQCRSGVCISLHWKCDGVDDCGD-YSDEANCRASCIICWSSGPTVAPRCLPLFQFQCRNHRCVPVWWKCDNDNDCGDWSDEDTCPSKLPSGCPAHQFRCGDGTCIFDHFV-CDGSPDCADGSDEYGCPTQHAVITGRPS 935 HSP 3 Score: 57.3806 bits (137), Expect = 6.481e-8 Identity = 41/128 (32.03%), Postives = 58/128 (45.31%), Query Frame = -2
Query: 7678 QRACRNSICIDENKFCNGIKDCEDGFDEEINTC-----AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNC------IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8028
Q CRN C+ C+ DC D DE+ TC + C +QF C C+ +++ CDG DC +G DE C I + C + F C + T +P SA CD +C++G DE
Sbjct: 851 QFQCRNHRCVPVWWKCDNDNDCGDWSDED--TCPSKLPSGCPAHQFRCGD-------GTCIFDHFVCDGSPDCADGSDEYGCPTQHAVITGRPSPCSFTQFQCLKDKTCVPASARCDGRKDCVDGSDE 969
BLAST of SMED30010499 vs. Ensembl Nematostella
Match: EDO49498 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGB0]) HSP 1 Score: 79.7221 bits (195), Expect = 1.198e-14 Identity = 50/143 (34.97%), Postives = 68/143 (47.55%), Query Frame = -2
Query: 7669 YECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8097
Y+C+ +L +A V CS + C N C + C+G DC DG DE+ C P QFSC P +C+ WRCDG+ DC +G DE C C S F+C N I + CD + +C + DE+NC
Sbjct: 67 YDCLAPDLSDEANCSVATNCSASMFRCANGQCKPRDWVCDGFDDCGDGSDEKGCANHSCTPAQFSC-------PSGRCIPLRWRCDGDGDCSDGADERGCP---PKNCTDSQFSC-SNGQCISLAWRCDGDHDCADKSDERNC 198 HSP 2 Score: 63.5438 bits (153), Expect = 1.025e-9 Identity = 40/121 (33.06%), Postives = 58/121 (47.93%), Query Frame = -2
Query: 7678 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
C+ +Q +C N CI C+G DC D DE T C+P +F+C + C+ W CDGENDCG+ DE++C +C + C + I +S CD +C + DE
Sbjct: 164 CTDSQFSCSNGQCISLAWRCDGDHDCADKSDERNCTGKTCKPFEFACAN------GRHCIQRKWICDGENDCGDRSDEVDC---GLESCGNDRWRCSNTSRCIAKSQVCDGRVDCPDASDE 275 HSP 3 Score: 58.5362 bits (140), Expect = 4.502e-8 Identity = 47/129 (36.43%), Postives = 59/129 (45.74%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDC--GNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS + C+N CI C+G DC D DE E CA+ C ++F C +KC+ WRCDG+ DC + DE NC C S F C N PR CD +C +G DEK C
Sbjct: 1 CSAGKFTCKNGHCISLRWKCDGENDCVDNSDEDEYAGCAEWSCRASEFRCQA------SRKCIPLSWRCDGDYDCLAPDLSDEANCSV--ATNCSASMFRC-ANGQCKPRDWVCDGFDDCGDGSDEKGC 120
BLAST of SMED30010499 vs. Ensembl Nematostella
Match: EDO49499 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RGB1]) HSP 1 Score: 79.337 bits (194), Expect = 1.947e-14 Identity = 46/112 (41.07%), Postives = 64/112 (57.14%), Query Frame = -2
Query: 7678 NSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8013
+S CI C+G+ +CEDG DE+ C P++F+C + G C+A+ W CDG+NDCG+ DELNC +L TC + F C N IP+SA CD +C + DE
Sbjct: 132 SSHCIPRRWLCDGLAECEDGSDEKNCQKFTCAPDKFACASGG-------CIASRWVCDGDNDCGDNSDELNCTRL---TCPPTKFLC-ANGMCIPKSAVCDGENDCGDMSDE 232 HSP 2 Score: 68.9366 bits (167), Expect = 2.822e-11 Identity = 48/142 (33.80%), Postives = 70/142 (49.30%), Query Frame = -2
Query: 7666 VHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCG-NGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR 8088
VH +L +A K+ S + + + CI ++ C+G DC D DEE C +F+C+ +CV W+CDGE DC G+DE C + TC ++F C ++ IPR CD C +G DEKNC+
Sbjct: 32 VHAKILQQA----KNCGSRHFKCVSDGKCIPKSWRCDGEMDCPDSSDEEGCVNRTCSSKEFNCN--------NQCVPLSWKCDGEKDCRPGGFDEEGCEPV---TCASTYFLCPNSSHCIPRRWLCDGLAECEDGSDEKNCQ 158 HSP 3 Score: 60.8474 bits (146), Expect = 1.008e-8 Identity = 41/123 (33.33%), Postives = 56/123 (45.53%), Query Frame = -2
Query: 7678 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ-CEPN-QFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
C + C N +CI ++ C+G DC D DE N A C+P +F C + +C+ WRCDG DC +G DE C + TC + C IP S CD +C + DE
Sbjct: 201 CPPTKFLCANGMCIPKSAVCDGENDCGDMSDEPSNCSAHICDPKLEFQC-------ANGRCINKKWRCDGMKDCADGSDESTC---GEGTCRPDEWHCIGTSRCIPLSRVCDGTNDCGDNYDE 313 HSP 4 Score: 55.0694 bits (131), Expect = 4.721e-7 Identity = 31/79 (39.24%), Postives = 44/79 (55.70%), Query Frame = -2
Query: 7786 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLD 8019
C N CI++ C+G+KDC DG DE C P+++ H +G +C+ CDG NDCG+ YDE +C+ LD
Sbjct: 249 CANGRCINKKWRCDGMKDCADGSDESTCGEGTCRPDEW--HCIG----TSRCIPLSRVCDGTNDCGDNYDEGSHCLDLD 321
BLAST of SMED30010499 vs. Ensembl Nematostella
Match: EDO43726 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RXU8]) HSP 1 Score: 75.485 bits (184), Expect = 2.404e-13 Identity = 57/179 (31.84%), Postives = 74/179 (41.34%), Query Frame = -2
Query: 7639 VKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA----------------QCEPNQFSCHTVGGKP--------------PHQ-----------KCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQ 8052
V+ C+ NQ C N CI + C+G DC DG DE+ + CA +C P+Q+ C P P + +CV +WRCDGE DC +G DE C K+ C S F C N IP S CD NC + DEK C P +P +
Sbjct: 116 VQRTCASNQFTCSNGDCISNSWTCDGDNDCNDGSDEKESLCASKSCKITEFTCRTSRRKCIPSQWKCDGDNDCPDSSDESGCPTASVSPRRCSVGMFKCRNGECVLGHWRCDGEKDCSDGSDEKGCR---KSNCASSEFTC-ANGQCIPSSQRCDGTSNCRDSSDEKACVTPPPCMPGE 290 HSP 2 Score: 64.3142 bits (155), Expect = 6.553e-10 Identity = 40/121 (33.06%), Postives = 59/121 (48.76%), Query Frame = -2
Query: 7678 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
CS CRN C+ + C+G KDC DG DE+ + C ++F+C + +C+ + RCDG ++C + DE C+ C+ F C IP S CD +C +GEDE
Sbjct: 207 CSVGMFKCRNGECVLGHWRCDGEKDCSDGSDEKGCRKSNCASSEFTC-------ANGQCIPSSQRCDGTSNCRDSSDEKACVT--PPPCMPGEFKCQSTGRCIPESKVCDGTRDCQDGEDE 318 HSP 3 Score: 62.003 bits (149), Expect = 3.291e-9 Identity = 41/124 (33.06%), Postives = 61/124 (49.19%), Query Frame = -2
Query: 7675 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEK 8040
C +Q CRN CI C+G DC DG+ + + C C ++F C ++KC++ W CD ++DCG+ DE + + TC + F C N I S CD + +C +G DEK
Sbjct: 40 CLQDQFTCRNGKCIQATWKCDGEDDCRDGYRSDESNCGNVTCGADEFMCS-------NRKCISRSWTCDNQDDCGDNSDEDRNV---QRTCASNQFTC-SNGDCISNSWTCDGDNDCNDGSDEK 152 HSP 4 Score: 62.003 bits (149), Expect = 4.211e-9 Identity = 41/125 (32.80%), Postives = 59/125 (47.20%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 8040
C ++ C N CI + C+ DC D DE+ N C NQF+C + C++N W CDG+NDC +G DE + K +C + F C + S W CD + +C + DE C
Sbjct: 81 CGADEFMCSNRKCISRSWTCDNQDDCGDNSDEDRNVQRTCASNQFTCS-------NGDCISNSWTCDGDNDCNDGSDEKESLCASK-SCKITEFTCRTSRRKCIPSQWKCDGDNDCPDSSDESGC 197 HSP 5 Score: 60.077 bits (144), Expect = 1.303e-8 Identity = 48/126 (38.10%), Postives = 62/126 (49.21%), Query Frame = -2
Query: 7678 CSFNQRAC-RNSICIDENKFCNGIKDCEDGFDEEIN--TCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGY--DELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
CS Q C + CI C+G DC DG DE+ + TC Q +QF+C + KC+ W+CDGE+DC +GY DE NC + TC F C N I RS CD +C + DE
Sbjct: 1 CSHGQFECVSDQKCIVLRWRCDGEDDCSDGSDEQGSPKTCLQ---DQFTCR-------NGKCIQATWKCDGEDDCRDGYRSDESNCGNV---TCGADEFMC-SNRKCISRSWTCDNQDDCGDNSDE 112
BLAST of SMED30010499 vs. Ensembl Nematostella
Match: EDO31103 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SYW2]) HSP 1 Score: 75.485 bits (184), Expect = 3.771e-13 Identity = 86/327 (26.30%), Postives = 134/327 (40.98%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQ---------SPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFV--GKMYQDFVVD-PNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQ-NVNGKSLYWCTNDNSEVIYS--NPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVD--HVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQN-----QCVK-KCPINCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
CL+C CSG + +CS A GY + + +P T G + + ++ SI ++ Y+F +DF P +LT Y + F +D+ P + +V K Y+ NSE++ S P G ++ + NW I +T A +++ VLS ++YL IR ++ +N T +D I N S+G+ ++ +E C CP Y G CE+C GY + N CV C + CD ++G C C +G C +C G SSDC EC C
Sbjct: 856 CLRCHCSGFSTNCSMASGYTRGQLISYLLLTADP---TDLDGWTGRYPNGQLASNLIFSSISNLPGGFYRFYIEASSARDFYFSAPTKYLTR------------GRYAYTHAMTFSLQQDNTTIPSSSTRGDVIIKGRYF----NSELVTSLPTPPGLTFTTYKVVFLESNWRIGNTSGRVATDKELVRVLSSLEYLWIRGRW-TNLTDPSLKTRLMDITMTIANKSSIGDTVF--NVETCQCPIQYVGQFCERCAPGYTRATPNGGAYTPCVPCSCNNHTDTCDPESGVCIDCQHNTTGDHCEKCAVGLYGDATNGTSSDCRECPC 1160 HSP 2 Score: 40.817 bits (94), Expect = 1.093e-8 Identity = 44/150 (29.33%), Postives = 61/150 (40.67%), Query Frame = -2
Query: 6289 NCPTPYTGLSCEKCLAGY---EKNDQNQCVKKCPINC-VECDKNGNCNRCTGQ-------RSGPKCLECKPGFVRLYGEKLSSDCTECNC------YSQLNNT--------LVSNI----CMRDL----TGQGAIVCKCTGRERNDPLCD 6639
NC + G CE+C GY + N +KC N V+ GNCN TG+ SG C C GF +G + C CNC + + NN LV+ + C++D +G+G C C + ND CD
Sbjct: 1186 NCSESHAGRQCERCQDGYYGTPTDPVNGQCRKCNCNGNVDPGVTGNCNTTTGECLKCLYNTSGFNCQWCVSGF---HGNATAKTCQACNCTDRGSKHGECNNVTGQCDCHPLVTGLSCERCVKDAFNYTSGKGCTACGCHPQGSNDTQCD 1332 HSP 3 Score: 39.2762 bits (90), Expect = 1.093e-8 Identity = 14/39 (35.90%), Postives = 20/39 (51.28%), Query Frame = -3
Query: 6069 VDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKT 6185
V G C C N KG C +C P F+ K C+ C+ ++
Sbjct: 1334 VFGNCTCNTNVKGDKCDQCMPNFYDVGKSCIGVSCTARS 1372
BLAST of SMED30010499 vs. Ensembl Nematostella
Match: EDO45218 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RTH9]) HSP 1 Score: 73.9442 bits (180), Expect = 1.091e-12 Identity = 45/124 (36.29%), Postives = 60/124 (48.39%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C+ Q C N CI + C+ DC D DE + C P+QF C + +C+++ WRCD +NDCG+ DE NC TC F C N IP S CD + +C + DE NC
Sbjct: 950 CAPGQFKCGNGKCIPSSWKCDHDNDCGDNSDENNCPYSTCNPSQFKCD-------NGRCISSKWRCDHDNDCGDMSDERNCT----GTCAPGQFKC-GNGKCIPSSWKCDHDNDCGDNSDENNC 1061 HSP 2 Score: 71.633 bits (174), Expect = 6.589e-12 Identity = 43/124 (34.68%), Postives = 63/124 (50.81%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C+ Q C N CI + C+ DC D DE + C P+QF C + +C+++ WRCD +NDCG+ DE NC +TC ++F C N IP CD + +C + DE++C
Sbjct: 1027 CAPGQFKCGNGKCIPSSWKCDHDNDCGDNSDENNCPYSTCNPSQFKCD-------NGRCISSKWRCDHDNDCGDMSDERNCT---FSTCASNYFRC-ANQRCIPMRWVCDFDNDCRDNSDERDC 1139 HSP 3 Score: 69.3218 bits (168), Expect = 3.199e-11 Identity = 44/129 (34.11%), Postives = 61/129 (47.29%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C+ N C N CI C+ DC D DE T + C N F C +Q+C+ W CD +NDC + DE +C + +C F+C N I RS CD + +C +G DE+NC +P
Sbjct: 1105 CASNYFRCANQRCIPMRWVCDFDNDCRDNSDERDCTPTFSTCASNYFRC-------ANQRCIPMRWVCDFDNDCRDNSDERDCTPTGR-SCNSGQFSC-SNGRCISRSWVCDRDNDCGDGSDERNCSYP 1224 HSP 4 Score: 67.3958 bits (163), Expect = 1.157e-10 Identity = 46/149 (30.87%), Postives = 70/149 (46.98%), Query Frame = -2
Query: 7594 NSICIDENKFCNGIKDCEDGFDEEINTC-------AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIK-LDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVI-PNQETTYVVHANSSISLK 8013
+ +CI + CNG DC D +DE+ C C P ++ C + C+ + + CDG C +G DE C + ++C G F CD N +P CD + +C + DE NC P +V P+ ETT +SI+L+
Sbjct: 1341 SGLCISTSWLCNGRVDCPDAWDEQPAQCRTSPAPTRTCSPQEYQCD-------NGACIPSRYECDGRIQCSDGSDETGCTATISPSSCPG--FLCDGNTLCLPFYKRCDGSYDCKDYTDEFNCSMPLMVSNPHVETT----TPTSITLR 1476 HSP 5 Score: 61.2326 bits (147), Expect = 8.196e-9 Identity = 35/96 (36.46%), Postives = 50/96 (52.08%), Query Frame = -2
Query: 7669 FDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAW-CDENPNCLNGEDEKNC 7953
F ++ T C P QF C + KC+ + W+CD +NDCG+ DE NC +TC S F CD+ + S W CD + +C + DE+NC
Sbjct: 940 FHQDAQTAGTCAPGQFKCG-------NGKCIPSSWKCDHDNDCGDNSDENNC---PYSTCNPSQFKCDNGRCI--SSKWRCDHDNDCGDMSDERNC 1023 HSP 6 Score: 54.6842 bits (130), Expect = 7.280e-7 Identity = 41/137 (29.93%), Postives = 56/137 (40.88%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEE--INTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKN-----------TCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C+ N C N CI C+ DC D DE T C QFSC + +C++ W CD +NDCG+G DE NC +C F+C + + R CD +C + DE +C
Sbjct: 1146 CASNYFRCANQRCIPMRWVCDFDNDCRDNSDERDCTPTGRSCNSGQFSCS-------NGRCISRSWVCDRDNDCGDGSDERNCSYPTTGPWTTRPWTPIFSCRSWEFSCLNGRCVFYRLV-CDGVDDCGDSSDEMSC 1274
BLAST of SMED30010499 vs. Ensembl Medaka
Match: hspg2 (heparan sulfate proteoglycan 2 [Source:NCBI gene;Acc:101159991]) HSP 1 Score: 208.764 bits (530), Expect = 3.922e-66 Identity = 175/621 (28.18%), Postives = 265/621 (42.67%), Query Frame = -2
Query: 6289 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAM--AKDYTIFVTSKTLCEKPMFNNAAMFV----SMCLKCFCSGQTDDCSSAMGY-------------------YQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIR--LFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWE-KENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCP-INC----VECDK-NGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAI-VCKC--TGRERNDPLCD 8040
C +Q C++ CI + C+G +DC DG DE T + CEPN+F C + +C WRCDG+NDC + DE++C K + C HF C + IP S CDE P+C + DE C P V P +E+ V S++ C V VPT I WR NWG +P +++ LTI ++ G YTCEA++ K + D + + S + P+ N C+ CFC+G + +C SA Y Y S + LL+ + ++ V ++ +++S F P FL G+ ++SY LK +R L + + P VIL NG+ L + S P NQ +I E ++NW + S R +++ L+++ + IR Y ++ + ++ T S+G A +E+C CP YTGLSCE C +G+E+ + C NC +CD +GNC C GP+C +C+PG+ G DC C C + S+ C D+ Q C+ TGR CD
Sbjct: 183 CRADQATCQSGECISRDYICDGERDCADGSDEFRCTPSPCEPNEFRCK-------NGRCALKLWRCDGDNDCEDNSDEMDCTKGPEEQCASKHFECVSDRKCIPASYQCDEEPDCADRSDEYGC-PPTVTTPPEESIQAVRG-STVMFTCRAVGVPTPMITWRLNWGHIPVSGRVSMNNKNG-----FGTLTIRDVKESDQGAYTCEAINAKGLVFGVPDGVLTLVSNPI---PLSGNCPDGTFSVNDRCVPCFCAGISKNCKSAGRYRDQIRLRFTDEEDFKVNVSYPSRPSTPPLSSTQLLIKPEMEEFQLVDLSRRFLNLDS------------FWTLPRQFL-------GNKIDSYGGTLKFKVRYTLARGLSESVERPDVILVG-NGRRLVYRRGQ------STPANVVNQKEIRFTEFQDNWQHSNGQPVS--RGELMMTLANLDSISIRTIYDNHMVSVALSDIVMETTTTEFISLGP---ARDVEEC-CPAGYTGLSCEICSSGFERVPGGLYLGTCAGCNCNGHATDCDPISGNCLSCQHNTEGPQCEKCRPGYFGDPGRGRPDDCKPCPC-PYPETSRFSDTCFLDIDQQATCDACRPGYTGRRCESSKCD 753 HSP 2 Score: 66.2402 bits (160), Expect = 3.922e-66 Identity = 83/332 (25.00%), Postives = 131/332 (39.46%), Query Frame = -3
Query: 5301 ENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKK----CLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTL----------LYGGKISFTFEY-------NTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGF---VKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDC 6224
E+ C++ GT + C CK N G +C EC PGFF + CL CFC ++ C S +R + +L LSNR NTN T + + +R P++I + T ++PDDI+ + L YGG++ +T + + D D+V +E + +T+ F E WR +G+ RE + AL I + + + DV++ D+ + H+ N++ VE C CP G G C+ C +VGY + I G C+ DC
Sbjct: 748 ESSKCDNRGTVSSSSRPCSCKNNVAGASCDECKPGFFHLSQANPQGCLSCFCMGVSKTCA-SSTWRRDQQVRGGVNGQL-FSLSNR--------ANTN-------TISEGISQRGPSEIVYRFFT------SLPDDIYYWNLPESFRGDKVTAYGGELHYTVRFEPFQRSQSVDNQPDVVLQGNDILLEY-------YHPIKLIPRVAQTVTVTFRES---AWRR-SDGQPCT-REHLLMALADVDVFMIRATYADNLAESISDVQM-DIAVPHSTG-NEVA--LEVEECACPLGYRGPSCQEC-EVGYSRTRS------GIYLGTCERCDC 1033 HSP 3 Score: 82.4185 bits (202), Expect = 1.026e-14 Identity = 78/301 (25.91%), Postives = 122/301 (40.53%), Query Frame = -3
Query: 5385 NCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQK----KCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLL----------YGGKISFTFEY---NTDLLKD-----LVFSDVTPFIEIESKKF-GRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHA-KWMNDLGKIAPVEMCLCPKGNPGIKCEAC 6215
NC+ G++ G C CK N +G +CS C PG F + CL CFC TQ+C S YR D V S G +E N+ NR V+ + L Y ++ H + L YGGK+ +T Y +T L+D ++ +D+T ++F GR +TK E +F E++ WR + +L RE + L + I + + ++ + D+++E A + L + VE C CP G G+ C+ C
Sbjct: 1143 NCDQTGSQGCTGGTCYCKMNVEGSSCSSCKPGAFHLHQDNKEGCLSCFCMGVTQQCSSSTHYR-------------DTVTSAF-FPGNFEGFALVNRQRTNRISTGFTVEVSTEGTQLSYSNFDYLG----EEPHYWQLPEAYKGDKVVSYGGKLKYTISYVAESTKSLQDDPDVQIIGNDITLVARQPWRQFPGR---------QTKHFEVVFREEH---WR--RPDGILATREHLMMVLADLDDILIRASY-SADMRSSSISDVSMEIAVPNYSGLAQALEVEQCRCPPGYQGLSCQDC 1410 HSP 4 Score: 70.0922 bits (170), Expect = 5.054e-11 Identity = 71/318 (22.33%), Postives = 123/318 (38.68%), Query Frame = -2
Query: 6403 CLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFK---SQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESK---DDIPGPKVILQNVNGKSLY-WCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECD--------KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNC 7311
CL CFC G T CSS+ Y + TS G F Q+ +S ++ +V+++ + + +P+ + + G + SY LK I ES D P ++I ++ + W + + F + W + + + A R ++ VL+D+ +LIR Y ++ + +++ + N + + +E+C CP Y GLSC+ C GY + + C + EC+ + G C C+ G C C PGF +DC C C
Sbjct: 1186 CLSCFCMGVTQQCSSSTHYRDT---VTSAFFPGNFEGFALVNRQRTNRISTGFTV-EVSTEGTQLSYSNFDYLGEEPHYWQLPE-AYKGDKVVSYGGKLKYTISYVAESTKSLQDDPDVQIIGNDITLVARQPWRQFPGRQTKH-----FEVVFREEHWRRPDGIL-------ATREHLMMVLADLDDILIRASYSADMRSSSISDVSMEIAVPNYSGLAQAL---EVEQCRCPPGYQGLSCQDCAPGYTRTGTGLYLGHCEL--CECNGHSDSCHPETGICTSCSHNTQGEFCELCAPGFFGDPTVGTPADCQLCAC 1481 HSP 5 Score: 60.8474 bits (146), Expect = 3.497e-8 Identity = 74/330 (22.42%), Postives = 121/330 (36.67%), Query Frame = -2
Query: 6403 CEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQK-ILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRL--FGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPI-----NCVECD-KNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSD---CTECNC 7356
C+ F+ + CL CFC G + C+S+ + + G L + ++ +S S + Y+F + D + G + +Y L +R F S+ P V+LQ G + ++I + + S W + + R +L L+D+ +IR Y N + V I P S GN + A +E+C CP Y G SC++C GY + + C + CD + G C +C G +C C+ G+ YG L+ D C C C
Sbjct: 779 CKPGFFHLSQANPQGCLSCFCMGVSKTCASSTWRR---DQQVRGGVNGQLFSLSNRANTNTISEGISQRGPSEIVYRFFTSLPDDIY-----YWNLPESFRGDKVTAYGGELHYTVRFEPFQRSQSVDNQPDVVLQ---GNDILLEYYHPIKLIPRVAQTVTVTFRESAWRRSDG-------QPCTREHLLMALADVDVFMIRATYADN---LAESISDVQMDIAVPHSTGNEV-ALEVEECACPLGYRGPSCQECEVGYSRTRSGIYLGTCERCDCNGHSSSCDPETGRCLQCLHNTDGQRCERCQNGY---YGNPLTGDPQACQPCPC 1083
BLAST of SMED30010499 vs. Ensembl Medaka
Match: ldlrb (low-density lipoprotein receptor [Source:NCBI gene;Acc:101161779]) HSP 1 Score: 83.9593 bits (206), Expect = 2.291e-15 Identity = 53/129 (41.09%), Postives = 65/129 (50.39%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
CS +Q C N C+ C+G DC DG DE ++TCA C P+QF+C G P +Q CV W CDG+ DC NG DE NC C F C N I S CD + +C +G DE C P
Sbjct: 30 CSSSQLKCGNGRCVTRRWICDGTDDCGDGTDELLSTCASKTCLPSQFNC----GAPINQ-CVPKSWHCDGKADCSNGADEKNCT---VKQCKNDEFRC-ANGQCISLSFVCDNDNDCSDGSDETTCSKP 149 HSP 2 Score: 67.3958 bits (163), Expect = 2.290e-10 Identity = 42/123 (34.15%), Postives = 61/123 (49.59%), Query Frame = -2
Query: 7669 CRNSICIDENKFCNGIKDCEDGFDEEINTC------AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8019
C NS+C+ C+G +DC DG DE TC A+C ++F C + +C+ WRCDG DC + DELNC + CL F C D + + + C+ +C +G DE +C
Sbjct: 158 CNNSVCVPSLWRCDGDEDCADGSDEWPETCGGKKPAAKCSVSEFEC-------ANGQCIPGSWRCDGGIDCDDRSDELNC---SRPVCLEGQFQCGDG-SCLQGNLKCNSVRDCSDGSDETDC 269 HSP 3 Score: 63.929 bits (154), Expect = 2.808e-9 Identity = 46/130 (35.38%), Postives = 58/130 (44.62%), Query Frame = -2
Query: 7660 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL--NC-IKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C ++ C N CI + C+ DC DG DE + C P F C+ + CV + WRCDG+ DC +G DE C K C S F C N IP S CD +C + DE NC P
Sbjct: 112 CKNDEFRCANGQCISLSFVCDNDNDCSDGSDETTCSKPTCNPLSFQCN-------NSVCVPSLWRCDGDEDCADGSDEWPETCGGKKPAAKCSVSEFEC-ANGQCIPGSWRCDGGIDCDDRSDELNCSRP 233
BLAST of SMED30010499 vs. Ensembl Medaka
Match: lrp2b (low density lipoprotein receptor-related protein 2b [Source:ZFIN;Acc:ZDB-GENE-111115-1]) HSP 1 Score: 79.7221 bits (195), Expect = 5.134e-14 Identity = 43/117 (36.75%), Postives = 63/117 (53.85%), Query Frame = -2
Query: 7669 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8019
C C + C+GI DC D DE C C P F+C+ ++ C+ + WRCDG +DCG+G DE+NC +TC +F C DN + ++ CD + +C +G DE+NC
Sbjct: 56 CDEGRCRPNSYRCDGIFDCVDKTDEA--NCTSCSPLAFTCN-------NKHCIYSSWRCDGMDDCGDGSDEVNCPTYIPSTCSSEYFTC-DNKRCVSKAWLCDGDNDCGDGSDERNC 162 HSP 2 Score: 75.8702 bits (185), Expect = 7.701e-13 Identity = 48/128 (37.50%), Postives = 65/128 (50.78%), Query Frame = -2
Query: 7666 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE---EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR 8040
CS C N CI + C+G+ DC DG DE + C F+C +++CV+ W CDG+NDCG+G DE NC +TC +F C D+ I CD + +CL+G DEKNC
Sbjct: 86 CSPLAFTCNNKHCIYSSWRCDGMDDCGDGSDEVNCPTYIPSTCSSEYFTCD-------NKRCVSKAWLCDGDNDCGDGSDERNC-NSSISTCRPGYFLCPDH-RCISSYFVCDGDQDCLDGSDEKNCE 204 HSP 3 Score: 73.1738 bits (178), Expect = 6.146e-12 Identity = 43/127 (33.86%), Postives = 62/127 (48.82%), Query Frame = -2
Query: 7666 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEI--NTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCR 8040
CS C N C+ + C+G DC DG DE ++ + C P F C P +C+++Y+ CDG+ DC +G DE NC + +C FAC + S CD +C + DE+NCR
Sbjct: 128 CSSEYFTCDNKRCVSKAWLCDGDNDCGDGSDERNCNSSISTCRPGYFLC-------PDHRCISSYFVCDGDQDCLDGSDEKNC----EFSCQSYQFACASGDQCVSSSYRCDGVFDCRDHSDERNCR 243 HSP 4 Score: 66.2402 bits (160), Expect = 6.215e-10 Identity = 46/129 (35.66%), Postives = 60/129 (46.51%), Query Frame = -2
Query: 7660 CSFNQRACRNS-ICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELN-CIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8040
C Q AC + C+ + C+G+ DC D DE C ++F C G C+ W CDG DC +G DE N C + TCL S+F C N IP S CD +C + DE+NC P
Sbjct: 207 CQSYQFACASGDQCVSSSYRCDGVFDCRDHSDERNCRPGLCHDDEFQCQMDG------FCIPANWECDGHPDCEDGSDEHNTCPAV---TCLSSYFQC-ANKMCIPMSWLCDGENDCRDMSDEQNCPTP 325 HSP 5 Score: 55.0694 bits (131), Expect = 1.859e-6 Identity = 50/165 (30.30%), Postives = 68/165 (41.21%), Query Frame = -2
Query: 7594 FCSFNQRACR-NSICIDENKFCNGIKDCEDGFDEEINTC--AQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLD---KNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP-EVVIP--------NQETTYVVHANSSISLK 8043
C ++ C+ + CI N C+G DCEDG DE NTC C + F C ++ C+ W CDGENDC + DE NC + C ++ C D P A C P D K C E +IP N+ T+ H + L+
Sbjct: 246 LCHDDEFQCQMDGFCIPANWECDGHPDCEDGSDEH-NTCPAVTCLSSYFQC-------ANKMCIPMSWLCDGENDCRDMSDEQNCPTPPFTYQEDCALNNGGCSDGCIQSPFGAQCTCPPGFQLLNDSKTCDDINECLIPGFCSQQCYNERGTFRCHCSDGYLLE 402
BLAST of SMED30010499 vs. Ensembl Medaka
Match: ENSORLT00000033251.1 (pep primary_assembly:ASM223467v1:8:21242553:21248942:-1 gene:ENSORLG00000021901.1 transcript:ENSORLT00000033251.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 74.3294 bits (181), Expect = 9.965e-14 Identity = 53/168 (31.55%), Postives = 76/168 (45.24%), Query Frame = -2
Query: 7549 CRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFS---CHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLD--KNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH-----PEVVIPNQETTYVVHAN-SSISLKCEVVSVPTSTINWRF 8019
C NSICI C+G DC DG DE +C P + CH++ + + W+CDG+ DC +G DE CI L ++TC F C D T I S C++ +C + DE C P ++P + T + H S+ L C + + S WR
Sbjct: 7 CNNSICIPRQSACDGYNDCLDGSDEWPQSCHAESPVTLASHQCHSMEFHCGSGEYIHGSWKCDGDADCLDGSDEAGCIALPLARSTCRPDEFECGDG-TCIHGSRQCNQQYDCRDTTDESGCVDGKRTLPRSIVPLKSCTLLTHHFIWSLLLLCRLQRM--SLQQWRL 171
BLAST of SMED30010499 vs. Ensembl Medaka
Match: lrp2a (LDL receptor related protein 2 [Source:NCBI gene;Acc:101157655]) HSP 1 Score: 75.8702 bits (185), Expect = 9.779e-13 Identity = 45/129 (34.88%), Postives = 66/129 (51.16%), Query Frame = -2
Query: 7654 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEV 8040
C+ Q C + CID N C+G +DC DG DE+ C C N+F C T G +C+++Y+ CDG DC + DE NC + S F C + + +P + CD +P+C +G DE + P
Sbjct: 1101 CNPEQFQCPDHRCIDPNYICDGDRDCVDGADEQ--DCYNCTANEFKC-TNG-----HQCISSYYHCDGVFDCSDRSDEHNCTRPPGMCHHESEFQCQSDGSCVPSTWECDGHPDCEDGSDEHHACPPRT 1221 HSP 2 Score: 75.8702 bits (185), Expect = 1.100e-12 Identity = 46/127 (36.22%), Postives = 65/127 (51.18%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE---EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
C N +C N C+ + C+G+ DC D DE +NT C P+ F+C +++CV WRCDG NDC + DE+NC TC + F C N IP + CD + +C +G DE +C
Sbjct: 980 CGANSFSCGNGKCVPNSYRCDGVDDCHDNSDEMSCGVNT--TCSPSAFTC-------ANRRCVPVGWRCDGHNDCLDDSDEINCPTRVPGTCPANQFTC-ANHRCIPHTWRCDTDNDCGDGSDEVDC 1096 HSP 3 Score: 72.0182 bits (175), Expect = 1.566e-11 Identity = 43/127 (33.86%), Postives = 65/127 (51.18%), Query Frame = -2
Query: 7669 CSFNQRACRNSICIDENKFCNGIKDCEDGFDE---EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNC 8040
CS + C N C+ C+G DC D DE C NQF+C + +C+ + WRCD +NDCG+G DE++C+ +TC F C D+ + P CD + +C++G DE++C
Sbjct: 1020 CSPSAFTCANRRCVPVGWRCDGHNDCLDDSDEINCPTRVPGTCPANQFTC-------ANHRCIPHTWRCDTDNDCGDGSDEVDCL---GSTCNPEQFQCPDHRCIDPNYI-CDGDRDCVDGADEQDC 1135 HSP 4 Score: 70.0922 bits (170), Expect = 6.034e-11 Identity = 41/123 (33.33%), Postives = 59/123 (47.97%), Query Frame = -2
Query: 7672 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKN 8040
C+ C+N +CI + C+ DC D DE +N C+ N F+C P +C+ W CDGE+DC + DE C + + C + C + IP CD +CL+GEDE N
Sbjct: 155 CNAGMFQCQNGLCIPQRYVCDHDDDCGDRSDE-LNCYPTCKGNYFTC-------PSGRCIHQVWLCDGEDDCEDNADEKGCDNVPRE-CYPGEWPCPSSGKCIPMDKLCDGRVDCLDGEDETN 268 HSP 5 Score: 68.5514 bits (166), Expect = 1.749e-10 Identity = 48/131 (36.64%), Postives = 64/131 (48.85%), Query Frame = -2
Query: 7657 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQ--CEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDEL-NCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPE 8040
C Q C CI + C+G DC D DE+ CAQ C+ + F C + G +C+ + W CD E DC +G DE NC + TC G F C N IP CD +C +G DE++C +PE
Sbjct: 3 CELGQFRCGTGRCIPGDWHCDGTSDCVDDSDEQ--DCAQVTCDHSHFQCLSDG------ECIPDVWVCDDEEDCEDGSDERQNCGR----TCTGDQFTC-SNGMCIPALYRCDHMADCSDGSDERHC-YPE 119 HSP 6 Score: 67.781 bits (164), Expect = 2.658e-10 Identity = 46/133 (34.59%), Postives = 68/133 (51.13%), Query Frame = -2
Query: 7654 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCI-KLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKN---CRHPEV 8040
C N+ C N +CI + +C+ DC D DE+ T C+P+QF+C + +CVA + CDG+NDCG+ DEL + + TC +F C +N I S CD + +C + DEK C P +
Sbjct: 2929 CGINEFTCSNGLCI-RSSYCDRRNDCGDSSDEQGCTYQPCQPHQFTCQ-------NGRCVAQDFVCDGDNDCGDESDELEHMCRTPAPTCPPGNFRC-ENGHCIDLSRVCDRSDDCSDNSDEKGINECTDPSI 3052 HSP 7 Score: 67.781 bits (164), Expect = 2.940e-10 Identity = 46/133 (34.59%), Postives = 66/133 (49.62%), Query Frame = -2
Query: 7663 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEIN--TCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNV----TLIPRSAWCDENPNCLNGEDE-KNCRH 8040
CS ++ C + CI + +C+G DC DG DE ++ C +QF C +C+A W CDG+NDCG+ DE + + TC S F C +N IPR CD + +C + DE +NC H
Sbjct: 2803 CSQSEFRCSSGRCIPAHWYCDGGSDCSDGSDEPLSCTLIRTCNTDQFRCDD-------GRCIALSWICDGDNDCGDMSDEDQ--RHNNRTCSSSEFTCANNQPPQRKCIPRDWVCDGDADCADALDEHQNCTH 2926 HSP 8 Score: 66.2402 bits (160), Expect = 7.388e-10 Identity = 45/145 (31.03%), Postives = 61/145 (42.07%), Query Frame = -2
Query: 7660 ECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHP 8094
+C+ ++ I V C NQ C N CI C+ DC DG DE + C P QF C P +C+ + CDG+ DC +G DE +C C + F C + I CD +C + DE NC P
Sbjct: 1044 DCLDDSDEINCPTRVPGTCPANQFTCANHRCIPHTWRCDTDNDCGDGSDEVDCLGSTCNPEQFQC-------PDHRCIDPNYICDGDRDCVDGADEQDCYN-----CTANEFKCTNGHQCISSYYHCDGVFDCSDRSDEHNCTRP 1176 HSP 9 Score: 65.0846 bits (157), Expect = 1.888e-9 Identity = 41/122 (33.61%), Postives = 59/122 (48.36%), Query Frame = -2
Query: 7678 CSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCA-QCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8040
C Q C + C CN +DC DG DE+ C QCE +Q+ C +++C+ W+CDGE+DCG+ DE + TC F C N IP++ CD + +C + DE
Sbjct: 3482 CHLGQFQCSDGNCTSPYFLCNSNQDCPDGSDEDPVLCTHQCESHQWQC-------ANKRCIPESWQCDGEDDCGDHSDE-DPAHCSARTCRPGQFKC-RNGRCIPQAWKCDVDDDCGDNSDE 3594 HSP 10 Score: 63.5438 bits (153), Expect = 5.032e-9 Identity = 43/138 (31.16%), Postives = 70/138 (50.72%), Query Frame = -2
Query: 7663 IKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDE-EINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDE-LNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRH 8070
+ TLI C+ +Q C + CI + C+G DC D DE + + C ++F+C +PP +KC+ W CDG+ DC + DE NC +C + F C + + + RS++CD +C + DE+ C +
Sbjct: 2836 LSCTLIRT--CNTDQFRCDDGRCIALSWICDGDNDCGDMSDEDQRHNNRTCSSSEFTC--ANNQPPQRKCIPRDWVCDGDADCADALDEHQNCT---HRSCGINEFTCSNGLCI--RSSYCDRRNDCGDSSDEQGCTY 2964 HSP 11 Score: 58.151 bits (139), Expect = 2.475e-7 Identity = 40/115 (34.78%), Postives = 54/115 (46.96%), Query Frame = -2
Query: 7678 CRNSICIDENKFCNGIKDCED-GFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDE 8019
C N CI + CNGI +C D G +E N C+P Q C + C+ + CDG+NDCG+ DE + +TC S F C IP +CD +C +G DE
Sbjct: 2728 CNNGRCIAKEYVCNGINNCYDNGTSDEQNCERTCQPEQTKCQST------NICIPRSYLCDGDNDCGDMSDE-SPTHCATSTCSQSEFRCSSG-RCIPAHWYCDGGSDCSDGSDE 2834
BLAST of SMED30010499 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1) HSP 1 Score: 4175.93 bits (10829), Expect = 0.000e+0 Identity = 2058/2081 (98.89%), Postives = 2068/2081 (99.38%), Query Frame = -3
Query: 3 AKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 6245
KKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSN SEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVR+VSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVI EVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQ+NIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHIN VVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVC+DSRPNSTSNITV+V NKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKY FIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWS VNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGK+VPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAK EVTGKKIVIKDVSVLKNMEIRVRIRNGTTDY+SKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGI NEK+NIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP+IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAV+INEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTF+YLWEIEENNGK TNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYP ISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAG EFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNL YPIKDLAENLIEK
Sbjct: 1084 CKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNGSEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRRVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVIPEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQRNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINRVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCRDSRPNSTSNITVNVLNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYIFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSTVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKKVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKVEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYSSKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPIIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEK 3164 HSP 2 Score: 1653.26 bits (4280), Expect = 0.000e+0 Identity = 779/788 (98.86%), Postives = 783/788 (99.37%), Query Frame = -2
Query: 6238 LKWQKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 8601
L+ KKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKK+VASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQF+CHTVGG PPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDS CETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCL GYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK
Sbjct: 299 LEMAKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKDVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFACHTVGGIPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSICETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLTGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 1086 HSP 3 Score: 582.793 bits (1501), Expect = 1.168e-166 Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = -1
Query: 8585 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNNTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 9430
ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCN TKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK
Sbjct: 23 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNTTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 304 HSP 4 Score: 196.052 bits (497), Expect = 3.769e-49 Identity = 243/958 (25.37%), Postives = 415/958 (43.32%), Query Frame = -3
Query: 3 PQLSVNVIYDPNKPPYPVAVVCKDSRPNST-------SNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNT----NNIFPAELSIFSTSPVINDK-IRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATV--------ESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGM--TNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYS-KESPFYPQKLICKDKTGVSSKVQVVKETGINNE-KVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRY-KQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKS-------GDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRS---RNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNL-QLTQGSNKTLQCFGLDSVTKKE-NNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVK-PNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPIS-KESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGS-NRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 2756
P L Y P YP ++CKD S+ + I + N Y G L N+ F+P+ + G Y I TN++ +LP+ T N E + N K + I L I C Y+G PI + V E + V S Y ++ ++FD K SG Y C++ + L+ ++ ++ G+++ + +G + + QG N D + F +WEI TN L+ KV K +++ V KN+ R + Y SK V E+ L P L+ YS E + L C D++G + +QV +G +++ KVN E L NS +P G+Y+C N++ I V +R +N + E ++ K ++ ++N I C Y G + P++ + E S GD VKK +F S + N TQ N+ + +A SD +NI + P I V + GLD ++ QGSN+ L C ++ + K+ + F++ W +E I+ L+E+ + G L VK P + L +C V SK F + +ED L++PKL + + + +K L+C+D+S + + + +++ + + +NK +N +E + +S++ LK+ G Y C A +K+G++ +D K + + +++ +K + K+ I ++ EIDCL+ GG+PP W+ K+S+ +L + + + V++K+ +IL+++ N+ + N L C VKD+ G S V + + V GLD + S F P QGS +R+L S+ + + KD+S F F W+ YPI LAE + +K
Sbjct: 2565 PDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNE-----GTL----DWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPI-IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTI---KGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDK-----GTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLI--ANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSE--NPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNE----IDCLYSGGKPPLV-ADWFLEKNSEMRKLPRGD--SVKKYKVFEKRSDILSIRTEN----ITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDRSEATSEMTLDYINRTLAGGVLNKKRNHIEYVFTTDSKSTLKYDGIYRCNATSKYGSNSITLDTKIKPVFKPLIVSVYSKDVNFTVKDKRNTAILPEYRVI-EIDCLHFGGQPPTDVTWYQIKDSQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSS-FNPKQGSMDRKLQASIIDAKTGKDLSGGFTFKWYFLQSNKEEYPIDKLAEKVDQK 3487 HSP 5 Score: 77.411 bits (189), Expect = 2.556e-13 Identity = 74/317 (23.34%), Postives = 150/317 (47.32%), Query Frame = -3
Query: 339 CRSRNATH-----SLSDRLNIKGIIPG-IDVTIKGLDTNNNLQLTQGS-NKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIK-VNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLN---EIDCLYSGGKPPLVADWFLEKNSEM-------RKLPRGDSVKKYKVFEK 1232
C + N TH S++ +N G + + GL + N GS ++ L+C+ D K + F++ W + + ++ I L++ V + G + +++ P + L +C Y S V ED +PKL + ++ + + + L CL++ E T I + ++G + + KN+NSV ++ P+ + +++G+Y C A+NK+GTD+ +++K + +S+N+ + I+ G + S++ N ++DC Y+ G+P + W++ + RK+P + K V E+
Sbjct: 5037 CFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDKKKDVSKDFKFTWYFLKADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTVVEDVDNRPKLHFSKTIGKLSDFPEKLLCLNERPETTQIYIGNINGKPIPKEWIQKNENSVTIMFPVEQSSISEYLGTYYCFAKNKYGTDNTTLEIK---GFEPLSVNVNSTINKINPNPGRADLLSTLVRSNRKLQLDC-YATGEPNVKIQWYIFTEKYVPDVPIPARKIPLNPNEKSQNVIER 5349
BLAST of SMED30010499 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1) HSP 1 Score: 4175.93 bits (10829), Expect = 0.000e+0 Identity = 2058/2081 (98.89%), Postives = 2068/2081 (99.38%), Query Frame = -3
Query: 3 AKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 6245
KKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSN SEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVR+VSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVI EVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQ+NIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHIN VVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVC+DSRPNSTSNITV+V NKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKY FIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWS VNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGK+VPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAK EVTGKKIVIKDVSVLKNMEIRVRIRNGTTDY+SKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGI NEK+NIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP+IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAV+INEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTF+YLWEIEENNGK TNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYP ISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAG EFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNL YPIKDLAENLIEK
Sbjct: 1084 CKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNGSEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRRVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVIPEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQRNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINRVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCRDSRPNSTSNITVNVLNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYIFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSTVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKKVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKVEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYSSKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPIIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEK 3164 HSP 2 Score: 1653.26 bits (4280), Expect = 0.000e+0 Identity = 779/788 (98.86%), Postives = 783/788 (99.37%), Query Frame = -2
Query: 6238 LKWQKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 8601
L+ KKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKK+VASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQF+CHTVGG PPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDS CETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCL GYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK
Sbjct: 299 LEMAKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKDVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFACHTVGGIPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSICETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLTGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 1086 HSP 3 Score: 582.793 bits (1501), Expect = 1.168e-166 Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = -1
Query: 8585 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNNTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 9430
ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCN TKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK
Sbjct: 23 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNTTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 304 HSP 4 Score: 196.052 bits (497), Expect = 3.769e-49 Identity = 243/958 (25.37%), Postives = 415/958 (43.32%), Query Frame = -3
Query: 3 PQLSVNVIYDPNKPPYPVAVVCKDSRPNST-------SNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNT----NNIFPAELSIFSTSPVINDK-IRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATV--------ESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGM--TNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYS-KESPFYPQKLICKDKTGVSSKVQVVKETGINNE-KVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRY-KQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKS-------GDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRS---RNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNL-QLTQGSNKTLQCFGLDSVTKKE-NNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVK-PNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPIS-KESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGS-NRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 2756
P L Y P YP ++CKD S+ + I + N Y G L N+ F+P+ + G Y I TN++ +LP+ T N E + N K + I L I C Y+G PI + V E + V S Y ++ ++FD K SG Y C++ + L+ ++ ++ G+++ + +G + + QG N D + F +WEI TN L+ KV K +++ V KN+ R + Y SK V E+ L P L+ YS E + L C D++G + +QV +G +++ KVN E L NS +P G+Y+C N++ I V +R +N + E ++ K ++ ++N I C Y G + P++ + E S GD VKK +F S + N TQ N+ + +A SD +NI + P I V + GLD ++ QGSN+ L C ++ + K+ + F++ W +E I+ L+E+ + G L VK P + L +C V SK F + +ED L++PKL + + + +K L+C+D+S + + + +++ + + +NK +N +E + +S++ LK+ G Y C A +K+G++ +D K + + +++ +K + K+ I ++ EIDCL+ GG+PP W+ K+S+ +L + + + V++K+ +IL+++ N+ + N L C VKD+ G S V + + V GLD + S F P QGS +R+L S+ + + KD+S F F W+ YPI LAE + +K
Sbjct: 2565 PDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNE-----GTL----DWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPI-IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTI---KGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDK-----GTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLI--ANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSE--NPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNE----IDCLYSGGKPPLV-ADWFLEKNSEMRKLPRGD--SVKKYKVFEKRSDILSIRTEN----ITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDRSEATSEMTLDYINRTLAGGVLNKKRNHIEYVFTTDSKSTLKYDGIYRCNATSKYGSNSITLDTKIKPVFKPLIVSVYSKDVNFTVKDKRNTAILPEYRVI-EIDCLHFGGQPPTDVTWYQIKDSQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSS-FNPKQGSMDRKLQASIIDAKTGKDLSGGFTFKWYFLQSNKEEYPIDKLAEKVDQK 3487 HSP 5 Score: 77.411 bits (189), Expect = 2.556e-13 Identity = 74/317 (23.34%), Postives = 150/317 (47.32%), Query Frame = -3
Query: 339 CRSRNATH-----SLSDRLNIKGIIPG-IDVTIKGLDTNNNLQLTQGS-NKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIK-VNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLN---EIDCLYSGGKPPLVADWFLEKNSEM-------RKLPRGDSVKKYKVFEK 1232
C + N TH S++ +N G + + GL + N GS ++ L+C+ D K + F++ W + + ++ I L++ V + G + +++ P + L +C Y S V ED +PKL + ++ + + + L CL++ E T I + ++G + + KN+NSV ++ P+ + +++G+Y C A+NK+GTD+ +++K + +S+N+ + I+ G + S++ N ++DC Y+ G+P + W++ + RK+P + K V E+
Sbjct: 5037 CFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDKKKDVSKDFKFTWYFLKADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTVVEDVDNRPKLHFSKTIGKLSDFPEKLLCLNERPETTQIYIGNINGKPIPKEWIQKNENSVTIMFPVEQSSISEYLGTYYCFAKNKYGTDNTTLEIK---GFEPLSVNVNSTINKINPNPGRADLLSTLVRSNRKLQLDC-YATGEPNVKIQWYIFTEKYVPDVPIPARKIPLNPNEKSQNVIER 5349
BLAST of SMED30010499 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1) HSP 1 Score: 4175.93 bits (10829), Expect = 0.000e+0 Identity = 2058/2081 (98.89%), Postives = 2068/2081 (99.38%), Query Frame = -3
Query: 3 AKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 6245
KKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSN SEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVR+VSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVI EVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQ+NIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHIN VVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVC+DSRPNSTSNITV+V NKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKY FIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWS VNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGK+VPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAK EVTGKKIVIKDVSVLKNMEIRVRIRNGTTDY+SKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGI NEK+NIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP+IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAV+INEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTF+YLWEIEENNGK TNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYP ISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAG EFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNL YPIKDLAENLIEK
Sbjct: 1084 CKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNGSEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRRVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVIPEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQRNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINRVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCRDSRPNSTSNITVNVLNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYIFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSTVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKKVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKVEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYSSKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPIIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEK 3164 HSP 2 Score: 1653.26 bits (4280), Expect = 0.000e+0 Identity = 779/788 (98.86%), Postives = 783/788 (99.37%), Query Frame = -2
Query: 6238 LKWQKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 8601
L+ KKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKK+VASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQF+CHTVGG PPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDS CETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCL GYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK
Sbjct: 299 LEMAKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKDVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFACHTVGGIPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSICETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLTGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 1086 HSP 3 Score: 582.793 bits (1501), Expect = 1.168e-166 Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = -1
Query: 8585 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNNTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 9430
ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCN TKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK
Sbjct: 23 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNTTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 304 HSP 4 Score: 196.052 bits (497), Expect = 3.769e-49 Identity = 243/958 (25.37%), Postives = 415/958 (43.32%), Query Frame = -3
Query: 3 PQLSVNVIYDPNKPPYPVAVVCKDSRPNST-------SNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNT----NNIFPAELSIFSTSPVINDK-IRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATV--------ESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGM--TNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYS-KESPFYPQKLICKDKTGVSSKVQVVKETGINNE-KVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRY-KQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKS-------GDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRS---RNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNL-QLTQGSNKTLQCFGLDSVTKKE-NNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVK-PNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPIS-KESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGS-NRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 2756
P L Y P YP ++CKD S+ + I + N Y G L N+ F+P+ + G Y I TN++ +LP+ T N E + N K + I L I C Y+G PI + V E + V S Y ++ ++FD K SG Y C++ + L+ ++ ++ G+++ + +G + + QG N D + F +WEI TN L+ KV K +++ V KN+ R + Y SK V E+ L P L+ YS E + L C D++G + +QV +G +++ KVN E L NS +P G+Y+C N++ I V +R +N + E ++ K ++ ++N I C Y G + P++ + E S GD VKK +F S + N TQ N+ + +A SD +NI + P I V + GLD ++ QGSN+ L C ++ + K+ + F++ W +E I+ L+E+ + G L VK P + L +C V SK F + +ED L++PKL + + + +K L+C+D+S + + + +++ + + +NK +N +E + +S++ LK+ G Y C A +K+G++ +D K + + +++ +K + K+ I ++ EIDCL+ GG+PP W+ K+S+ +L + + + V++K+ +IL+++ N+ + N L C VKD+ G S V + + V GLD + S F P QGS +R+L S+ + + KD+S F F W+ YPI LAE + +K
Sbjct: 2565 PDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNE-----GTL----DWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPI-IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTI---KGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDK-----GTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLI--ANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSE--NPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNE----IDCLYSGGKPPLV-ADWFLEKNSEMRKLPRGD--SVKKYKVFEKRSDILSIRTEN----ITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDRSEATSEMTLDYINRTLAGGVLNKKRNHIEYVFTTDSKSTLKYDGIYRCNATSKYGSNSITLDTKIKPVFKPLIVSVYSKDVNFTVKDKRNTAILPEYRVI-EIDCLHFGGQPPTDVTWYQIKDSQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSS-FNPKQGSMDRKLQASIIDAKTGKDLSGGFTFKWYFLQSNKEEYPIDKLAEKVDQK 3487 HSP 5 Score: 77.411 bits (189), Expect = 2.556e-13 Identity = 74/317 (23.34%), Postives = 150/317 (47.32%), Query Frame = -3
Query: 339 CRSRNATH-----SLSDRLNIKGIIPG-IDVTIKGLDTNNNLQLTQGS-NKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIK-VNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLN---EIDCLYSGGKPPLVADWFLEKNSEM-------RKLPRGDSVKKYKVFEK 1232
C + N TH S++ +N G + + GL + N GS ++ L+C+ D K + F++ W + + ++ I L++ V + G + +++ P + L +C Y S V ED +PKL + ++ + + + L CL++ E T I + ++G + + KN+NSV ++ P+ + +++G+Y C A+NK+GTD+ +++K + +S+N+ + I+ G + S++ N ++DC Y+ G+P + W++ + RK+P + K V E+
Sbjct: 5037 CFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDKKKDVSKDFKFTWYFLKADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTVVEDVDNRPKLHFSKTIGKLSDFPEKLLCLNERPETTQIYIGNINGKPIPKEWIQKNENSVTIMFPVEQSSISEYLGTYYCFAKNKYGTDNTTLEIK---GFEPLSVNVNSTINKINPNPGRADLLSTLVRSNRKLQLDC-YATGEPNVKIQWYIFTEKYVPDVPIPARKIPLNPNEKSQNVIER 5349
BLAST of SMED30010499 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1) HSP 1 Score: 4175.93 bits (10829), Expect = 0.000e+0 Identity = 2058/2081 (98.89%), Postives = 2068/2081 (99.38%), Query Frame = -3
Query: 3 AKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 6245
KKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSN SEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVR+VSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVI EVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQ+NIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHIN VVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVC+DSRPNSTSNITV+V NKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKY FIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWS VNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGK+VPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAK EVTGKKIVIKDVSVLKNMEIRVRIRNGTTDY+SKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGI NEK+NIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP+IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAV+INEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTF+YLWEIEENNGK TNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYP ISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAG EFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNL YPIKDLAENLIEK
Sbjct: 1084 CKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNGSEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRRVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVIPEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQRNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINRVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCRDSRPNSTSNITVNVLNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYIFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSTVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKKVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKVEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYSSKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPIIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEK 3164 HSP 2 Score: 1653.26 bits (4280), Expect = 0.000e+0 Identity = 779/788 (98.86%), Postives = 783/788 (99.37%), Query Frame = -2
Query: 6238 LKWQKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 8601
L+ KKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKK+VASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQF+CHTVGG PPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDS CETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCL GYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK
Sbjct: 299 LEMAKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKDVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFACHTVGGIPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSICETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLTGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 1086 HSP 3 Score: 582.793 bits (1501), Expect = 1.168e-166 Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = -1
Query: 8585 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNNTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 9430
ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCN TKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK
Sbjct: 23 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNTTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 304 HSP 4 Score: 196.052 bits (497), Expect = 3.769e-49 Identity = 243/958 (25.37%), Postives = 415/958 (43.32%), Query Frame = -3
Query: 3 PQLSVNVIYDPNKPPYPVAVVCKDSRPNST-------SNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNT----NNIFPAELSIFSTSPVINDK-IRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATV--------ESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGM--TNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYS-KESPFYPQKLICKDKTGVSSKVQVVKETGINNE-KVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRY-KQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKS-------GDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRS---RNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNL-QLTQGSNKTLQCFGLDSVTKKE-NNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVK-PNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPIS-KESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGS-NRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 2756
P L Y P YP ++CKD S+ + I + N Y G L N+ F+P+ + G Y I TN++ +LP+ T N E + N K + I L I C Y+G PI + V E + V S Y ++ ++FD K SG Y C++ + L+ ++ ++ G+++ + +G + + QG N D + F +WEI TN L+ KV K +++ V KN+ R + Y SK V E+ L P L+ YS E + L C D++G + +QV +G +++ KVN E L NS +P G+Y+C N++ I V +R +N + E ++ K ++ ++N I C Y G + P++ + E S GD VKK +F S + N TQ N+ + +A SD +NI + P I V + GLD ++ QGSN+ L C ++ + K+ + F++ W +E I+ L+E+ + G L VK P + L +C V SK F + +ED L++PKL + + + +K L+C+D+S + + + +++ + + +NK +N +E + +S++ LK+ G Y C A +K+G++ +D K + + +++ +K + K+ I ++ EIDCL+ GG+PP W+ K+S+ +L + + + V++K+ +IL+++ N+ + N L C VKD+ G S V + + V GLD + S F P QGS +R+L S+ + + KD+S F F W+ YPI LAE + +K
Sbjct: 2565 PDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNE-----GTL----DWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPI-IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTI---KGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDK-----GTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLI--ANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSE--NPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNE----IDCLYSGGKPPLV-ADWFLEKNSEMRKLPRGD--SVKKYKVFEKRSDILSIRTEN----ITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDRSEATSEMTLDYINRTLAGGVLNKKRNHIEYVFTTDSKSTLKYDGIYRCNATSKYGSNSITLDTKIKPVFKPLIVSVYSKDVNFTVKDKRNTAILPEYRVI-EIDCLHFGGQPPTDVTWYQIKDSQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSS-FNPKQGSMDRKLQASIIDAKTGKDLSGGFTFKWYFLQSNKEEYPIDKLAEKVDQK 3487 HSP 5 Score: 77.411 bits (189), Expect = 2.556e-13 Identity = 74/317 (23.34%), Postives = 150/317 (47.32%), Query Frame = -3
Query: 339 CRSRNATH-----SLSDRLNIKGIIPG-IDVTIKGLDTNNNLQLTQGS-NKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIK-VNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLN---EIDCLYSGGKPPLVADWFLEKNSEM-------RKLPRGDSVKKYKVFEK 1232
C + N TH S++ +N G + + GL + N GS ++ L+C+ D K + F++ W + + ++ I L++ V + G + +++ P + L +C Y S V ED +PKL + ++ + + + L CL++ E T I + ++G + + KN+NSV ++ P+ + +++G+Y C A+NK+GTD+ +++K + +S+N+ + I+ G + S++ N ++DC Y+ G+P + W++ + RK+P + K V E+
Sbjct: 5037 CFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDKKKDVSKDFKFTWYFLKADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTVVEDVDNRPKLHFSKTIGKLSDFPEKLLCLNERPETTQIYIGNINGKPIPKEWIQKNENSVTIMFPVEQSSISEYLGTYYCFAKNKYGTDNTTLEIK---GFEPLSVNVNSTINKINPNPGRADLLSTLVRSNRKLQLDC-YATGEPNVKIQWYIFTEKYVPDVPIPARKIPLNPNEKSQNVIER 5349
BLAST of SMED30010499 vs. Planmine SMEST
Match: SMESG000055070.1 (SMESG000055070.1) HSP 1 Score: 4175.55 bits (10828), Expect = 0.000e+0 Identity = 2058/2081 (98.89%), Postives = 2068/2081 (99.38%), Query Frame = -3
Query: 3 AKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNASEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDXXXXXXXXXXXXXVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRKVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVISEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQKNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINSVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCKDSRPNSTSNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGINNEKVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 6245
KKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSN SEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVR+VSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVI EVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQ+NIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHIN VVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVC+DSRPNSTSNITV+V NKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKY FIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWS VNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGK+VPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAK EVTGKKIVIKDVSVLKNMEIRVRIRNGTTDY+SKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGI NEK+NIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAP+IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAV+INEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTF+YLWEIEENNGK TNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYP ISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAG EFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNL YPIKDLAENLIEK
Sbjct: 1084 CKKRDLSENKNCNSLGTENIVDGKCKCKENYKGPNCSECSPGFFSFQKKCLKCFCSNKTQECKESDQYRFSNLTLNSADIKLDVVLSNRQISGVYEAQNTNNQLLVNRTENIILVKRNPNQIGLVYVTLKLTNSTVPDDIHKYTLLYGGKISFTFEYNTDLLKDLVFSDVTPFIEIESKKFGRLWTVLSFNEKTKTMESLFMEDNFPKWRIGKEGKLLEDRETFIRALMTAQHLSIGFPFDNGFVKDVRLRDLTIEHAKWMNDLGKIAPVEMCLCPKGNPGIKCEACNKVGYENVINVSPNLPDIVCGRCDSPDCADCKEGTYKYDFVLNKNFSTCQIGIEMMTKGRIIYVNKDESIRIPCTAKSHRGGIAIHKWNKVSESTHLNVTHEMTEDKSSNLILNFKKIQMSDGGLYVCEAVNSVSNGSEEFFIIVEEKPPRLAVEISVTTIDKVKTQVNVTVDSPQKPTDYKVFWFLVKDGEKDAKETSSKISMTTENKVYKIDIEVPTNVTTGVKLKGVIVGSRVENPFRFVIEENIKRNLSINSDQLKVSVDKPVLEVNFLESARVRVLVNPKIRTETFWYKVNEDGSEHPLPNGVTKDVNDLLIWSAGDNHNGRYVGYIINSDEGTKVGKVETTVKVAPMRPVVNKEILNTTEPRNASISLYVPDPIKCPSQSWELVDPKTKPIDITHLVRRVSDKEIIIDRELSIGQVIRFRCSVIPEIDMKGKIKITIDSKFPVVEIKKKFETSVYPVSMECRDNNTAHPSSVVWYFKGKPVDKNLVINTNSSSTINWKIVGGFSLDLHEGIYTCRINNSFNSASKSVPVPDSKTSGSRHSKYPGTINITSSTNPIFKQQQALSIFVTKKQRLEITAELFGNPANDSQLEWLVNGKITEPISRSKYYSKLVIPEVKDQDHLGIYEVRVKLENGTVISRKIEISYPKETSIYLKVDGINPSGNMTILEGDTKSVRCSLIKSVKGEESEIIDANIVWKIKTTMGQQIPVSELPGFTKEITPKELKISKANRTLNSYIGQCTGTYENKNYESRFIFFDVNQRNIDMKVRLLGLIDNKFVSNVEEDTSQVICQAYDARSNRALPGVKYSWNMTHINRVVNWKKLIEDNDKITFGGLIYHPADNSAVGVCKADYFNTIMYSEPFSFKVIKKDRTETPQLSVNVIYDPNKPPYPVAVVCRDSRPNSTSNITVNVLNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYIFIATNKFGTDSKTILLPQHNNTNNIFPAELSIFSTSPVINDKIRIQGDTSLKIICVYFGHPYPISLKWSTVNEYQLSGATVESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKKVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGMTNYSLLTTDKVNAKVEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYSSKTVVVNIIENELLHPDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPIIDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDKGTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEK 3164 HSP 2 Score: 1652.88 bits (4279), Expect = 0.000e+0 Identity = 779/788 (98.86%), Postives = 783/788 (99.37%), Query Frame = -2
Query: 6238 LKWQKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKNVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFSCHTVGGKPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSTCETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGNNIYAPRIEKCNCPTPYTGLSCEKCLAGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 8601
L+ KKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKK+VASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQF+CHTVGG PPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDS CETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMG+NIYAPRIEKCNCPTPYTGLSCEKCL GYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK
Sbjct: 299 LEMAKKTFSVNIYPNNVSVFEDMPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVKPFHAGTYYCDAKKDVASAVLNVLPKPTLLVTPTEMIVRPGSSARFLCQLIGASGTVIWRREDKKSFQIDVESRVGGETQALLIVNTATKEMNGTVYECVHNNLLIKATLIVKDFCSFNQRACRNSICIDENKFCNGIKDCEDGFDEEINTCAQCEPNQFACHTVGGIPPHQKCVANYWRCDGENDCGNGYDELNCIKLDKNTCLGSHFACDDNVTLIPRSAWCDENPNCLNGEDEKNCRHPEVVIPNQETTYVVHANSSISLKCEVVSVPTSTINWRFNWGRLPQRLNFTVHQTGKDCNRITNVLTINNMSPDFAGIYTCEALSTKRAMAKDYTIFVTSKTLCEKPMFNNAAMFVSMCLKCFCSGQTDDCSSAMGYYQSPNPKTSTRTKGLLMNFKSQKILEVSYAKSIYDVNSKSYKFVGKMYQDFVVDPNVFLTSDFGMDGSWLESYSYYLKINIRLFGESKDDIPGPKVILQNVNGKSLYWCTNDNSEVIYSNPEGFYNQLKISLWEKENWFIDSICETSAMRPDILAVLSDIKYLLIRIKYYSNQTGFEFFNATVDHVITNPDSMGSNIYAPRIEKCNCPTPYTGLSCEKCLTGYEKNDQNQCVKKCPINCVECDKNGNCNRCTGQRSGPKCLECKPGFVRLYGEKLSSDCTECNCYSQLNNTLVSNICMRDLTGQGAIVCKCTGRERNDPLCDKCLQEPLSIADERDCKK 1086 HSP 3 Score: 583.178 bits (1502), Expect = 1.127e-166 Identity = 281/282 (99.65%), Postives = 281/282 (99.65%), Query Frame = -1
Query: 8585 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNNTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 9430
ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCN TKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK
Sbjct: 23 ERFRVFSVKTSDQLFTHVMIRCPLAWSEDDTITFLGEYSGIIIPIPNFGESKDSTIVDVASEKTLTTYYCTVKHKADGSEESVKFYFDKENKYQDIQQLAYSWTPVEIINTELDQPLTITCNTTKPNIKTIWFGPDLLQFNHTENTLTINSVKVTDLGSYFCADELNIIETILLAKRIIVKLPTVEYNALSRKTVQSTNFPTSPLGESLQYLTNRGFTTTRRVIWEQPIGLVLLNQGRSFYVFVFTPQYLNDEFFNQVTDRSDPNFQYSNKVLFHSLEMAKK 304 HSP 4 Score: 195.667 bits (496), Expect = 4.182e-49 Identity = 243/958 (25.37%), Postives = 415/958 (43.32%), Query Frame = -3
Query: 3 PQLSVNVIYDPNKPPYPVAVVCKDSRPNST-------SNITVDVFNKYFDQKRVLSKPGFLMINFTENNTKFNPYNNSGKYTFIATNKFGTDSKTILLPQHNNT----NNIFPAELSIFSTSPVINDK-IRIQGDTSLKIICVYFGHPYPISLKWSPVNEYQLSGATV--------ESSDYVTSLIYDKFDRKINSGEYTCQAISHQGLLTKKVQVESFGYGLEIDLLNEEGKEVPEVIVKKQGENIVFIYNETDLSDNSKPTKFSGVWEIRHPGM--TNYSLLTTDKVNAKFEVTGKKIVIKDVSVLKNMEIRVRIRNGTTDYASKTVVVNIIENELLHPDLVFVTEYS-KESPFYPQKLICKDKTGVSSKVQVVKETGINNE-KVNIYLTHNEGTLDWNSHPFSPFFDQGTYKCIGTNRY-KQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPMIDVEIVYEAKS-------GDLFVVKKPVLFSNYSQYYAAVQINEFDAKTQSGNYLCRS---RNATHSLSDRLNIKGIIPGIDVTIKGLDTNNNL-QLTQGSNKTLQCFGLDSVTKKE-NNTFQYLWEIEENNGKQTNIETLSESKVFDKGTLILNVVK-PNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPIS-KESGMKYIKSSIGLLNEIDCLYSGGKPPLVADWFLEKNSEMRKLPRGDSVKKYKVFEKRSDILSIRTENITQVMNFKLKCFVKDSAGREFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGS-NRQLICSVKETDSNKDVSKDFKFDWFVETQTNLRYPIKDLAENLIEK 2756
P L Y P YP ++CKD S+ + I + N Y G L N+ F+P+ + G Y I TN++ +LP+ T N E + N K + I L I C Y+G PI + V E + V S Y ++ ++FD K SG Y C++ + L+ ++ ++ G+++ + +G + + QG N D + F +WEI TN L+ KV K +++ V KN+ R + Y SK V E+ L P L+ YS E + L C D++G + +QV +G +++ KVN E L NS +P G+Y+C N++ I V +R +N + E ++ K ++ ++N I C Y G + P++ + E S GD VKK +F S + N TQ N+ + +A SD +NI + P I V + GLD ++ QGSN+ L C ++ + K+ + F++ W +E I+ L+E+ + G L VK P + L +C V SK F + +ED L++PKL + + + +K L+C+D+S + + + +++ + + +NK +N +E + +S++ LK+ G Y C A +K+G++ +D K + + +++ +K + K+ I ++ EIDCL+ GG+PP W+ K+S+ +L + + + V++K+ +IL+++ N+ + N L C VKD+ G S V + + V GLD + S F P QGS +R+L S+ + + KD+S F F W+ YPI LAE + +K
Sbjct: 2565 PDLVFVTEYSKESPFYPQKLICKDKTGVSSKVQVVKETGIKNEKINIYLTHNE-----GTL----DWNSHPFSPFFDQGTYKCIGTNRYKQRFIYTVLPEKRETTTPMNEYVQFESRVNEIKVEENKKTLEIINGNKLLISCNYYGTEAPI-IDVEIVYEAKSGDLFVVKKPVLFSNYSQYYAAVEINEFDAKTQSGNYLCRSRNATHSLSDRLNIKGIIPGIDVTI---KGLDTNNNLQLTQGSNKTLQCFGLDSVTKKENNTFKYLWEIEENNGKPTNIETLSESKVFDK-----GTLILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLI--ANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIKVNKNKNSVELILPINSE--NPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPPISINIITKSGPISKESGMKYIKSSIGLLNE----IDCLYSGGKPPLV-ADWFLEKNSEMRKLPRGD--SVKKYKVFEKRSDILSIRTEN----ITQVMNFKLKCFVKDSAGTEFSDSVNIIYVKPIITVDVFGLDHEGSMFTPLQGSNRQLICSVKETDSNKDVSKDFKFDWFVETQTNLHYPIKDLAENLIEKDGKLSFMTVKNPRQVLYAQCRVNNGSVNDVSKPFFIHVDEDELKRPKLFLKPTFEGELNAVKRLDCIDRSEATSEMTLDYINRTLAGGVLNKKRNHIEYVFTTDSKSTLKYDGIYRCNATSKYGSNSITLDTKIKPVFKPLIVSVYSKDVNFTVKDKRNTAILPEYRVI-EIDCLHFGGQPPTDVTWYQIKDSQEVELLKSEIKDNFNVYQKQPNILTVKILNMKKNSNMTLNCVVKDANGVMISSPVFLTQSPSGVRFVVAGLDGDSS-FNPKQGSMDRKLQASIIDAKTGKDLSGGFTFKWYFLQSNKEEYPIDKLAEKVDQK 3487 HSP 5 Score: 77.0258 bits (188), Expect = 2.853e-13 Identity = 74/317 (23.34%), Postives = 150/317 (47.32%), Query Frame = -3
Query: 339 CRSRNATH-----SLSDRLNIKGIIPG-IDVTIKGLDTNNNLQLTQGS-NKTLQCFGLDSVTKKENNTFQYLWEIEENNGKQTNIETLSESKVFDKGTL-ILNVVKPNKNLRGRCIVIVQGKKYFSKYFEVFTNEDALRKPKLIANYSVDEKTGIIKALECLDQSGEMTNIQVTFLSGSSSDIK-VNKNKNSVELILPINSENPLKFIGSYECKAENKFGTDHRIVDVKYVQRYPQISINIITKSGPISKESGMKYIKSSIGLLN---EIDCLYSGGKPPLVADWFLEKNSEM-------RKLPRGDSVKKYKVFEK 1232
C + N TH S++ +N G + + GL + N GS ++ L+C+ D K + F++ W + + ++ I L++ V + G + +++ P + L +C Y S V ED +PKL + ++ + + + L CL++ E T I + ++G + + KN+NSV ++ P+ + +++G+Y C A+NK+GTD+ +++K + +S+N+ + I+ G + S++ N ++DC Y+ G+P + W++ + RK+P + K V E+
Sbjct: 5139 CFATNGTHNFTSPSITVDVNKAETQTGPFTIWVSGLSGDFNFYPKVGSVDQKLRCYIEDDKKKDVSKDFKFTWYFLKADKQEYPIMDLADKVVINGGEISFMSMKNPQQVLTAQCFATKGAHNYTSTRISVTVVEDVDNRPKLHFSKTIGKLSDFPEKLLCLNERPETTQIYIGNINGKPIPKEWIQKNENSVTIMFPVEQSSISEYLGTYYCFAKNKYGTDNTTLEIK---GFEPLSVNVNSTINKINPNPGRADLLSTLVRSNRKLQLDC-YATGEPNVKIQWYIFTEKYVPDVPIPARKIPLNPNEKSQNVIER 5451 The following BLAST results are available for this feature:
BLAST of SMED30010499 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 5
BLAST of SMED30010499 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of SMED30010499 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010499 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of SMED30010499 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of SMED30010499 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010499 ID=SMED30010499|Name=SMED30010499|organism=Schmidtea mediterranea sexual|type=transcript|length=9532bpback to top protein sequence of SMED30010499-orf-1 >SMED30010499-orf-1 ID=SMED30010499-orf-1|Name=SMED30010499-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=326bp QFYTVWENLSFSMSSINPIFILTLLTIINSLNGIERFRVFSVKTSDQLFTback to top protein sequence of SMED30010499-orf-2 >SMED30010499-orf-2 ID=SMED30010499-orf-2|Name=SMED30010499-orf-2|organism=Schmidtea mediterranea sexual|type=polypeptide|length=2101bp MIPCVINAYRNLCQLLMNETAKKRDLSENKNCNSLGTENIVDGKCKCKENback to top protein sequence of SMED30010499-orf-3 >SMED30010499-orf-3 ID=SMED30010499-orf-3|Name=SMED30010499-orf-3|organism=Schmidtea mediterranea sexual|type=polypeptide|length=773bp MPVEFLCNNTFNYGSLWTFKNLASNVETNLSKDAVIRHNSFNSTVNINSVback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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