SMED30010488

Overview
NameSMED30010488
Smed IDSMED30010488
Length (bp)1298
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010488 (SMED30010488) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010488 (SMED30010488) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010488 (SMED30010488) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010488 (SMED30010488) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010488

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 19

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
intestinal phagocyteSMED30010488SMESG000050407.1 Contig983GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30010488SMESG000003538.1 PL010001001C01ncbi_smed_estsPMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
intestinal phagocyteSMED30010488SMESG000003538.1 Contig983GPL15192PMID:23079596
Forsthoefel et al., 2012		FACS sorted cell population asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30010488SMESG000003538.1 SMED30010488smed_20140614PMID:29906446
Zeng et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
epidermisSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle cellSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
non-ciliated neuronSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cilated neuronSMED30010488SMESG000003538.1 dd_Smed_v4_70_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010488SMESG000003538.1 Contig29339newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010488SMESG000003538.1 Contig29339uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
progenitor cellSMED30010488SMESG000003538.1 dd_Smed_v6_70_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010488SMESG000003538.1 dd_Smed_v6_70_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010488SMESG000003538.1 dd_Smed_v6_70_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
pharynx progenitor cellSMED30010488SMESG000003538.1 dd_Smed_v6_70_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
muscle progenitor cellSMED30010488SMESG000003538.1 dd_Smed_v6_70_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010488 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.001774:7155..8438 -6081
Homology
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BLAST of SMED30010488 vs. Planmine SMEST
Match: SMESG000003538.1 (SMESG000003538.1)

HSP 1 Score: 249.98 bits (637), Expect = 1.913e-82
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 1 
Query:   28 MAWASWDSNLDSYMASSFTECXXXXXXXXXXXXCTSRSKEAGPTPDANEIAALVKYLTSKQGASLTYGGNKLMFTREIEDSILIFNIIGANKGALAISKTASGNFVILACGAGPDKNSQAVGVNAVKCVEFLNKSL 435
            MAWASWDSNLDSYMASSFTECNNIAIAATNGAICTSRSKEAGPTPDANEIAALVKYLTSKQGASLTYGGNKLMFTREIEDSILIFNIIGANKGALAISKTASGNFVILACGAGPDKNSQAVGVNAVKCVEFLNKSL
Sbjct:    1 MAWASWDSNLDSYMASSFTECNNIAIAATNGAICTSRSKEAGPTPDANEIAALVKYLTSKQGASLTYGGNKLMFTREIEDSILIFNIIGANKGALAISKTASGNFVILACGAGPDKNSQAVGVNAVKCVEFLNKSL 136
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BLAST of SMED30010488 vs. Planmine SMEST
Match: SMESG000062027.1 (SMESG000062027.1)

HSP 1 Score: 145.206 bits (365), Expect = 6.290e-42
Identity = 81/138 (58.70%), Postives = 101/138 (73.19%), Query Frame = 1 
Query:   28 MAWASWDSNLDSYMASSFTECXXXXXXXXXXX-XCTSRSKEAGPTPDANEIAALVKYLTSKQGAS-LTYGGNKLMFTREIEDSILIFNIIGANKGALAISKTASGNFVILACGAGPDKNSQAVGVNAVKCVEFLNKSL 435
            MAWA+WD+NLD+YM ++F ECN++ IA  +G  ICTSR+K+ GPTP   EI AL  ++ SK     L YG  KLMFTR+IEDSI IFN  G  KGA+A  K+ASG+F+I+A GAG DKNSQ VGV A++ VEFL  SL
Sbjct:    1 MAWAAWDNNLDTYMNNNFPECNSVTIATESGDCICTSRTKDNGPTPTVTEITALASFIKSKSSPDGLVYGNYKLMFTRDIEDSICIFNFKGDIKGAIAACKSASGSFLIIAGGAGADKNSQGVGVQAIRFVEFLKNSL 138
The following BLAST results are available for this feature:
BLAST of SMED30010488 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010488 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000003538.11.913e-82100.00SMESG000003538.1[more]
SMESG000062027.16.290e-4258.70SMESG000062027.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010488 ID=SMED30010488|Name=SMED30010488|organism=Schmidtea mediterranea sexual|type=transcript|length=1298bp
GAAATCTATATAAATCTTATATGAAAAATGGCTTGGGCATCTTGGGATTC
AAATTTAGATAGCTACATGGCTTCAAGTTTCACTGAATGTAACAACATAG
CTATTGCTGCTACCAACGGTGCGATTTGCACATCTAGAAGTAAAGAAGCT
GGTCCAACTCCAGATGCGAACGAAATCGCTGCACTAGTTAAGTATCTTAC
TTCGAAACAAGGAGCATCTCTCACATACGGAGGAAACAAATTGATGTTTA
CTAGAGAAATTGAAGATTCAATCTTGATTTTCAATATTATTGGAGCTAAC
AAAGGAGCGCTAGCAATAAGCAAAACAGCTAGTGGAAACTTTGTTATTCT
TGCTTGTGGAGCTGGACCTGACAAAAATTCTCAAGCTGTAGGTGTTAATG
CCGTCAAGTGCGTCGAATTTTTGAACAAATCGTTGTAAATGGCGTCTATT
GAAAACAATTCATTAGGATTGTATTATTTTTCAATACTGATTTTGATACG
AAAAACAAATTTAAAATATATTGTGGTTTTCTATTTTGGATGATTTTCAT
TTCTTATTGTACGATTAAATTAATAGATAATAAACTCGTTTAGAAATATG
TTCATAGCATAAGTCAATAATATTTTTTGAATTGCGTCAATATTTTTTAT
AAAATGGATTTGAGTTTTAATATGGCAGTTTTTAATATGCTTTTTCGGAA
TTTTTACGTGCCAATCTTTTATTGTGATTGTAATGTCGTTTGAGTGTTGT
GAAATAAATGTCTCGATTTTCAGCGCAGTAATCTTCGTGATTATTAATGA
CGCGGCATTTTAAACTTTGTTATCATTGACATTCAATACTTAAGAATTAA
TTTTTACAGGTAAATATTTATTAGTGAATTATGTAAAGCTTGATAAATGC
ACATTTTATGTCACGGTTATTCAATAATATTATTCAATTGATGAATATCT
GTACAAAAAATTTTGTTTAGATTATTAATTTCATCTGTCACACAGAGATA
ATAAACATTTGTTGACGTGTGCAACAAAAATTCCATTATGCCTGCTGGAA
AAGTTGTAGAAATAATTTCCAGTTAAAAACTGTTTAGTAGAGTTTCGGCG
GGTTCTGGCGGCAAATCGGTTAGAATGCGCACAAATGTTTACAATAATAA
ATCTGCAATTCTATGACGGAATTATTCGATGGATTTGATTGGCTGATTTT
GGAAATTGTTTTTGCGAATTTTCAATAATAGATTTTTTGTTTTGTTTGCA
GTTGACGGTGATTAAAAAATGGAATTATTTCAATGGAATGCAATGCTG
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protein sequence of SMED30010488-orf-1

>SMED30010488-orf-1 ID=SMED30010488-orf-1|Name=SMED30010488-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=137bp
MAWASWDSNLDSYMASSFTECNNIAIAATNGAICTSRSKEAGPTPDANEI
AALVKYLTSKQGASLTYGGNKLMFTREIEDSILIFNIIGANKGALAISKT
ASGNFVILACGAGPDKNSQAVGVNAVKCVEFLNKSL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000019muscle progenitor cell
PLANA:0000070intestinal phagocyte
PLANA:0000099neuron
PLANA:0000110pharynx progenitor cell
PLANA:0000120progenitor cell
PLANA:0000429neoblast
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR036140PFN_sf
IPR005455PFN
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005455ProfilinPFAMPF00235Profilincoord: 6..122
e-value: 6.6E-8
score: 32.9
NoneNo IPR availableGENE3DG3DSA:3.30.450.30coord: 3..137
e-value: 2.9E-8
score: 35.7
IPR036140Profilin superfamilySUPERFAMILYSSF55770Profilin (actin-binding protein)coord: 6..122