SMED30010479

Overview
NameSMED30010479
Smed IDSMED30010479
Length (bp)1641
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010479 (SMED30010479) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010479 (SMED30010479) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010479 (SMED30010479) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010479 (SMED30010479) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010479

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 6

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
cephalic gangliaSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000079160.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 dd_Smed_v4_9575_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
parenchymal cellSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000079160.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 dd_Smed_v4_9575_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
oocyteSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 SMESG000016792.1 Contig55793uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 SMESG000016792.1 Contig55793uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
oocyteSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 SMESG000016792.1 Contig55793newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
vitelline glandSMED30010479SMESG000079185.1 SMESG000079162.1 SMESG000042893.1 SMESG000042876.1 SMESG000042866.1 SMESG000016792.1 Contig55793newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010479 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.013007:10694..19732 -6347
Homology
[Close]
BLAST of SMED30010479 vs. Planmine SMEST
Match: SMESG000042893.1 (SMESG000042893.1)

HSP 1 Score: 739.184 bits (1907), Expect = 0.000e+0
Identity = 356/373 (95.44%), Postives = 365/373 (97.86%), Query Frame = 3 
Query:  201 KIEIKFTFHINESAGILLYGADFWNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFEIDALDLTNSSKLIHMKCNNKTSKLEIQQKNSAQWNSYPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNNRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILSSSNS 1319
            KIEIKFTFHINESAGILLYGAD WNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFE+DALDLTNS KLI MKCNNKTSKLEIQQKNSAQWN +PRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFC INYIETDFNFTDCADGT+FACLTNGYSNRTVRFGNDCKSWITEDSNC+EDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYK+ISLIQILLN+ SNILLYENNY VFYIHNR+PLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQD+NCSTFEGLLR RFDPPE+FEILSSSNS
Sbjct:    2 KIEIKFTFHINESAGILLYGADLWNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFELDALDLTNSRKLILMKCNNKTSKLEIQQKNSAQWNRFPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCWINYIETDFNFTDCADGTQFACLTNGYSNRTVRFGNDCKSWITEDSNCEEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKVISLIQILLNDGSNILLYENNYKVFYIHNRYPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDQNCSTFEGLLRARFDPPEIFEILSSSNS 374
[Close]
BLAST of SMED30010479 vs. Planmine SMEST
Match: SMESG000042893.1 (SMESG000042893.1)

HSP 1 Score: 696.427 bits (1796), Expect = 0.000e+0
Identity = 340/373 (91.15%), Postives = 349/373 (93.57%), Query Frame = 3 
Query:  201 KIEIKFTFHINESAGILLYGADFWNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFEIDALDLTNSSKLIHMKCNNKTSKLEIQQKNSAQWNSYPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNNRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILSSSNS 1319
            KIEIKFTFHINESAGILLYGAD WNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFE+DALDLTNS KLI MKCNNKTSKLEIQQKNSAQWN +PRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFC INYIETDFNFTDCADGT+FACLTNGYSNRTVRFGNDCKSWITEDSNC+EDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYK+ISLIQILLN+ SNILLYENNY VFYIHNR+PLKLMRFTVK                SINDGEKIFTFHGYSIGNVSRINIGLPVQD+NCSTFEGLLR RFDPPE+FEILSSSNS
Sbjct:    2 KIEIKFTFHINESAGILLYGADLWNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSFYFELDALDLTNSRKLILMKCNNKTSKLEIQQKNSAQWNRFPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCWINYIETDFNFTDCADGTQFACLTNGYSNRTVRFGNDCKSWITEDSNCEEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKVISLIQILLNDGSNILLYENNYKVFYIHNRYPLKLMRFTVK----------------SINDGEKIFTFHGYSIGNVSRINIGLPVQDQNCSTFEGLLRARFDPPEIFEILSSSNS 358
[Close]
BLAST of SMED30010479 vs. Planmine SMEST
Match: SMESG000042876.1 (SMESG000042876.1)

HSP 1 Score: 636.721 bits (1641), Expect = 0.000e+0
Identity = 338/345 (97.97%), Postives = 342/345 (99.13%), Query Frame = 3 
Query:  510 AQWNSYPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNNRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILSSSNSSNNVPKGHXXXXXXXXXXXXXXXXXXXXKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 1544
            AQWNSYPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPP++EQIIGCLEDLYFNNKSVTFKKIGTKVLPFC INYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLN+RSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALS+NDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLR RFDPPELFEILSSSNSSNNVPKG YFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF
Sbjct:   24 AQWNSYPRPDKMDNNRNKTYIWMGNIGPYVSKVNQSYPPIKEQIIGCLEDLYFNNKSVTFKKIGTKVLPFCWINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNDRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSVNDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRARFDPPELFEILSSSNSSNNVPKGQYFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 368
[Close]
BLAST of SMED30010479 vs. Planmine SMEST
Match: SMESG000042876.1 (SMESG000042876.1)

HSP 1 Score: 611.298 bits (1575), Expect = 0.000e+0
Identity = 327/334 (97.90%), Postives = 331/334 (99.10%), Query Frame = 3 
Query:  543 MDNNRNKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNNRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILSSSNSSNNVPKGHXXXXXXXXXXXXXXXXXXXXKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 1544
            MDNNRNKTYIWMGNIGPYVSKVNQSYPP++EQIIGCLEDLYFNNKSVTFKKIGTKVLPFC INYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLN+RSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALS+NDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLR RFDPPELFEILSSSNSSNNVPKG YFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF
Sbjct:    1 MDNNRNKTYIWMGNIGPYVSKVNQSYPPIKEQIIGCLEDLYFNNKSVTFKKIGTKVLPFCWINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNDRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSVNDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRARFDPPELFEILSSSNSSNNVPKGQYFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 334
[Close]
BLAST of SMED30010479 vs. Planmine SMEST
Match: SMESG000042876.1 (SMESG000042876.1)

HSP 1 Score: 588.186 bits (1515), Expect = 0.000e+0
Identity = 316/323 (97.83%), Postives = 320/323 (99.07%), Query Frame = 3 
Query:  576 MGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTKVLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNNRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSINDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILSSSNSSNNVPKGHXXXXXXXXXXXXXXXXXXXXKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 1544
            MGNIGPYVSKVNQSYPP++EQIIGCLEDLYFNNKSVTFKKIGTKVLPFC INYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLN+RSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALS+NDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLR RFDPPELFEILSSSNSSNNVPKG YFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF
Sbjct:    1 MGNIGPYVSKVNQSYPPIKEQIIGCLEDLYFNNKSVTFKKIGTKVLPFCWINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITEDSNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNDRSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSVNDGEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRARFDPPELFEILSSSNSSNNVPKGQYFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATENVSNSEMDLDYFVLSDNSEYGFVCDKRIVF 323
The following BLAST results are available for this feature:
BLAST of SMED30010479 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010479 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 5
Match NameE-valueIdentityDescription
SMESG000042893.10.000e+095.44SMESG000042893.1[more]
SMESG000042893.10.000e+091.15SMESG000042893.1[more]
SMESG000042876.10.000e+097.97SMESG000042876.1[more]
SMESG000042876.10.000e+097.90SMESG000042876.1[more]
SMESG000042876.10.000e+097.83SMESG000042876.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010479 ID=SMED30010479|Name=SMED30010479|organism=Schmidtea mediterranea sexual|type=transcript|length=1641bp
TACATCTAAATGACAGAATTTATTTATTAATAATTTTACATCATTTGCAT
CATTATCAACACAAATGGAATATGAAATTTATTTATCAAATTTAACATAA
ATCTGAAATGTTGCAAATATTTGCATGGATTTGGTGGCTCCGATATTTTA
TCCATATTCGAGCAGATTATTTGCTCGGTGAAATAGATGAAAATCTCCAC
AAAATCGAGATAAAATTCACATTCCATATTAACGAATCAGCGGGAATCCT
TCTATATGGAGCGGATTTTTGGAATAATTCTTTGCTAATCAGAATTCGTC
ATTTACCCAATATTCAGAAACAGCTGGAAATTAATGTCAACGACAATCAA
GTGAAAGACAATATTAGGAATCTTTGCAACTGTTCAAAATGTTTTGATAA
AAGCTTTTATTTTGAAATTGATGCTCTGGATTTAACAAATTCAAGCAAAT
TGATTCACATGAAATGCAATAACAAAACTTCAAAGCTAGAAATCCAACAG
AAAAATTCAGCCCAGTGGAACAGTTATCCGAGACCAGACAAAATGGATAA
TAACAGGAATAAAACATACATTTGGATGGGAAATATCGGTCCATATGTCT
CAAAAGTGAATCAGTCGTATCCTCCCCTCAGAGAGCAAATCATTGGATGT
TTAGAAGACTTATATTTCAATAACAAATCTGTAACATTTAAAAAAATTGG
GACAAAGGTTTTGCCATTTTGCGGGATTAATTACATTGAAACCGATTTTA
ACTTTACTGATTGCGCTGATGGGACGCGATTTGCTTGCCTGACAAATGGA
TATAGCAACAGAACGGTCCGTTTCGGGAATGACTGCAAATCTTGGATTAC
AGAGGATTCCAACTGTAAGGAGGATTTGCCGCCATATAGCAACCAAAACC
AGTCATTCCTCATCAAGGGGTCACCAAGTCATTCATTTACAATACAATTG
ACATGGAAGCCGGTTCTCTACAAAATGATTTCATTAATTCAAATACTTTT
GAATAACAGGTCAAATATTTTATTATATGAAAATAATTATACGGTTTTCT
ATATTCACAATCGGCATCCGCTGAAATTGATGAGATTTACAGTGAAATCT
GTAAATAATTTGCTGTGGAATTTTACCAATAATCGATTTGCACTGAGTAT
AAATGATGGAGAGAAAATATTCACATTTCATGGATATTCAATAGGAAATG
TCTCCCGGATCAATATTGGGCTTCCGGTGCAGGATCGGAATTGTTCGACA
TTTGAAGGATTGTTAAGGGTCAGATTTGATCCGCCAGAATTATTTGAAAT
ATTATCCAGTAGCAATTCAAGTAATAACGTTCCAAAAGGACACTATTTTT
ATTATATTTTCATTATTTGTTTCATTAGTCTAGCCTTTATTATTATAGTT
ATTAAGGAGTATAAAACAAAAATAAAAAACCTTATGAAGAAACCCGCCAC
CGAAAATGTCAGTAATAGTGAAATGGACTTGGACTATTTTGTATTAAGTG
ACAATAGTGAATATGGTTTCGTTTGTGACAAAAGGATTGTTTTTTAAAAT
TTATATTCTCATTTATTAACGTAAGCGTTTTTATTTAAAACTTTTGTTAC
ATGTAATTTGTTTTGTTCATGTAATATTCAAAATTACTGTG
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protein sequence of SMED30010479-orf-1

>SMED30010479-orf-1 ID=SMED30010479-orf-1|Name=SMED30010479-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=480bp
MLQIFAWIWWLRYFIHIRADYLLGEIDENLHKIEIKFTFHINESAGILLY
GADFWNNSLLIRIRHLPNIQKQLEINVNDNQVKDNIRNLCNCSKCFDKSF
YFEIDALDLTNSSKLIHMKCNNKTSKLEIQQKNSAQWNSYPRPDKMDNNR
NKTYIWMGNIGPYVSKVNQSYPPLREQIIGCLEDLYFNNKSVTFKKIGTK
VLPFCGINYIETDFNFTDCADGTRFACLTNGYSNRTVRFGNDCKSWITED
SNCKEDLPPYSNQNQSFLIKGSPSHSFTIQLTWKPVLYKMISLIQILLNN
RSNILLYENNYTVFYIHNRHPLKLMRFTVKSVNNLLWNFTNNRFALSIND
GEKIFTFHGYSIGNVSRINIGLPVQDRNCSTFEGLLRVRFDPPELFEILS
SSNSSNNVPKGHYFYYIFIICFISLAFIIIVIKEYKTKIKNLMKKPATEN
VSNSEMDLDYFVLSDNSEYGFVCDKRIVF*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000074oocyte
PLANA:0000231vitelline gland
PLANA:0003116parenchymal cell
Vocabulary: INTERPRO
TermDefinition
IPR013320ConA-like_dom_sf
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..19
score: 0.667
NoneNo IPR availableTMHMMTMhelixcoord: 412..434