SMED30010448
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010448 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 1
Homology
BLAST of SMED30010448 vs. TrEMBL
Match: A0A401T1F0 (HTH CENPB-type domain-containing protein OS=Chiloscyllium punctatum OX=137246 GN=chiPu_0014986 PE=4 SV=1) HSP 1 Score: 65.4698 bits (158), Expect = 8.462e-20 Identity = 37/117 (31.62%), Postives = 65/117 (55.56%), Query Frame = -1 Query: 44 LAISGGIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLENDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFIQ 394 LA + G + V E D+ LL+S+GE L+NE+L +++ E D E P + L+ K + +A + + +E+ E+D + R V R N ++ Y++I R K+++ Q LD F + Sbjct: 759 LARAVGFDEVDETDVVELLESHGEELSNEDLMQLESQRAEEEDRIEIPPPQILSTKRLSRAFQIMEEAMEIFTEDDPNRERSAKVNRAINDGINCYKEIYRQKKKKTVQHSLDKFFK 875 HSP 2 Score: 60.077 bits (144), Expect = 8.462e-20 Identity = 24/56 (42.86%), Postives = 37/56 (66.07%), Query Frame = -2 Query: 436 KKLHQLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGE 603 + QLI ET G ++R +W+ +NI++ I NI +WNEVT SC+ VW++L+ E Sbjct: 682 RTFSQLIYETDGENAPSVREFWRGYNILEGINNIADSWNEVTSSCLNSVWRKLWPE 737
BLAST of SMED30010448 vs. TrEMBL
Match: K7G0J5 (HTH CENPB-type domain-containing protein OS=Pelodiscus sinensis OX=13735 PE=4 SV=1) HSP 1 Score: 58.5362 bits (140), Expect = 1.384e-18 Identity = 36/110 (32.73%), Postives = 63/110 (57.27%), Query Frame = -1 Query: 77 LAISGGIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLENDESEPIPV----RNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRRE 394 LA G + V EDD+ LL+S+GE LTNEEL +++ +E + E + V +NLT K + +A + +++L ++D D R + V + L+ Y++I + K+R+ Sbjct: 440 LAKKAGFDEVEEDDVTQLLQSHGEELTNEELMQLEAMRAMEEKDQE-VKVETTHQNLTAKRLSEAFQMIEAGLQILNDDDPDRERSSKVIKGVGHLMTCYKEIYQEKKRK 548 HSP 2 Score: 58.151 bits (139), Expect = 1.384e-18 Identity = 27/52 (51.92%), Postives = 36/52 (69.23%), Query Frame = -2 Query: 436 QLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGE 591 QLI T G GK +R +W +NI+K I NI KAW +VT++CM GVW +L+ E Sbjct: 367 QLIRGTEGEGKPTIRQFWHDYNILKCINNIGKAWADVTQACMNGVWAKLWPE 418 HSP 3 Score: 25.409 bits (54), Expect = 1.384e-18 Identity = 10/16 (62.50%), Postives = 11/16 (68.75%), Query Frame = -1 Query: 602 PKDQGTISALKVY*LK 649 P DQG ISA K Y L+ Sbjct: 347 PMDQGAISAFKAYYLR 362
BLAST of SMED30010448 vs. TrEMBL
Match: A0A4X2L2R9 (HTH CENPB-type domain-containing protein OS=Vombatus ursinus OX=29139 GN=LOC114040919 PE=4 SV=1) HSP 1 Score: 59.6918 bits (143), Expect = 3.374e-16 Identity = 37/104 (35.58%), Postives = 61/104 (58.65%), Query Frame = -1 Query: 71 GGIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLENDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAK 382 GG+EGVVE+D+ LL+S GE L+ E L T +N + P L+ + + +ALS +Q +++L +ND + R V R N+++ YR++ + K+R AK Sbjct: 430 GGLEGVVEEDVVHLLQSQGEDLSGEGLGMSSPGTGQDNTLATP---PELSTRHLAEALSHFDQGLQILSDNDPNRERSLKVCRGVNSVISCYRELYKEKKRAAK 530 HSP 2 Score: 54.299 bits (129), Expect = 3.374e-16 Identity = 25/65 (38.46%), Postives = 37/65 (56.92%), Query Frame = -2 Query: 409 KKLHQLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEITEIHSYYE 603 + L QLI ET G + +R +W+ +NIM + NI +AW E+ S M VWK+++ E S E Sbjct: 357 RTLCQLIQETDGEERPTIREFWRSYNIMTAVDNIAEAWQELKPSTMNAVWKKIWPECVRASSEAE 421
BLAST of SMED30010448 vs. TrEMBL
Match: A0A5S6QVR2 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 57.3806 bits (137), Expect = 1.262e-15 Identity = 41/129 (31.78%), Postives = 57/129 (44.19%), Query Frame = -1 Query: 32 RLAISGGIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLE-------NDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFIQRRAK 397 +LA G EGV DI +L + LT EELQE+ E N E P P R +T + L Q ++ LE+ND + R R A L+ Y+Q+L + AKQ L+ + K Sbjct: 378 QLANRLGFEGVEYQDIEDILSCQPDELTTEELQELSVAGEAEGREEDDENQEVPPPPPRQMTTAELSDTLETIEQRLQWLEDNDCNAERSGKTTRGIRACLEPYKQLLYERSTRAKQKKLEFYFTAMKK 506 HSP 2 Score: 48.1358 bits (113), Expect = 1.262e-15 Identity = 19/57 (33.33%), Postives = 34/57 (59.65%), Query Frame = -2 Query: 433 KKLHQLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEI 603 + LI+ T G + + +WK +N+ I NI +AWN+V+K C+ VW++L ++ Sbjct: 300 RTFKMLIDATDGENCETVMEFWKNYNLRMAINNIVEAWNDVSKRCLHRVWRKLIPDL 356 HSP 3 Score: 25.7942 bits (55), Expect = 1.262e-15 Identity = 13/34 (38.24%), Postives = 19/34 (55.88%), Query Frame = -1 Query: 596 IQFTYIQLTSLALDQVPKDQGTISALKVY*LKET 697 + F ++ + +L Q P DQG I A K Y L+ T Sbjct: 269 VHFVFLPARTTSLLQ-PLDQGVIGAFKAYYLRRT 301
BLAST of SMED30010448 vs. TrEMBL
Match: A0A5S6Q6E1 (Uncharacterized protein OS=Trichuris muris OX=70415 PE=4 SV=1) HSP 1 Score: 56.225 bits (134), Expect = 3.343e-15 Identity = 40/126 (31.75%), Postives = 60/126 (47.62%), Query Frame = -1 Query: 32 RLAISGGIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLE----NDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFIQRRAK 397 +LA G EGV DI +L + LT EELQE+ E +DE++ +P R +T + L Q ++ LE+ND + R R A L+ Y+Q+L + AKQ L+ + K Sbjct: 206 QLANKLGFEGVEYQDIEDILSCQPDELTTEELQELSVAGEAEGREEDDENQEVPPRQMTTAELSDTLETIEQRLQWLEDNDCNAERSGKTTRGIRACLEPYKQLLYERSTRAKQKKLEFYFTAMKK 331 HSP 2 Score: 45.8246 bits (107), Expect = 3.343e-15 Identity = 19/52 (36.54%), Postives = 33/52 (63.46%), Query Frame = -2 Query: 433 LINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEI 588 LI+ T G + + +WK +N+ I NI +AWN+V+K C+ VW++L ++ Sbjct: 133 LIDATDGENCETVMEFWKNYNLRMAINNIVEAWNDVSKRCLHCVWRKLIPDL 184 HSP 3 Score: 28.1054 bits (61), Expect = 3.343e-15 Identity = 13/34 (38.24%), Postives = 20/34 (58.82%), Query Frame = -1 Query: 596 IQFTYIQLTSLALDQVPKDQGTISALKVY*LKET 697 + F ++ + +L Q P DQG I+A K Y L+ T Sbjct: 97 VHFVFLPARTTSLLQ-PLDQGVIAAFKAYYLRRT 129
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003106.1 (pep scaffold:Pmarinus_7.0:GL476998:37517:41189:1 gene:ENSPMAG00000002845.1 transcript:ENSPMAT00000003106.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 49.6766 bits (117), Expect = 2.633e-15 Identity = 29/109 (26.61%), Postives = 56/109 (51.38%), Query Frame = -1 Query: 41 VVEDDIHTLLKSYGEPLTNEELQEIDCHTPLENDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFIQR 367 V +D+ LL S+ L+NE+L +++ E +++ P +L+ K + +A + LLE+ND ++ R + + YR+I R +++ A Q LD F ++ Sbjct: 374 VAPEDVGELLASHAHELSNEDLLQLEQQRLEEETQTQETPHPSLSTKVLSEAFQHFETGMALLEKNDPNFERSSKASEIIRSGYTCYREIYREQKKAAVQTSLDLFFKK 482 HSP 2 Score: 40.817 bits (94), Expect = 2.633e-15 Identity = 18/53 (33.96%), Postives = 31/53 (58.49%), Query Frame = -2 Query: 433 QLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEI 591 Q I+ T G + +WK F+I+ ++NI +W E+ +S + GVW++L E Sbjct: 293 QAISATDKEGGPTLTEFWKGFSILDAVKNIAASWKEMKQSNLNGVWRKLCPEF 345 HSP 3 Score: 26.9498 bits (58), Expect = 2.633e-15 Identity = 13/34 (38.24%), Postives = 21/34 (61.76%), Query Frame = -1 Query: 596 IQFTYIQLTSLALDQVPKDQGTISALKVY*LKET 697 I+ ++ + AL Q P DQG +S+ K Y L++T Sbjct: 258 IKVVFLTPNTTALLQ-PMDQGVMSSFKAYYLRQT 290
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000006798.1 (pep scaffold:Pmarinus_7.0:GL478691:31611:33359:1 gene:ENSPMAG00000006139.1 transcript:ENSPMAT00000006798.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 40.0466 bits (92), Expect = 3.267e-9 Identity = 19/56 (33.93%), Postives = 30/56 (53.57%), Query Frame = -2 Query: 436 KKLHQLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGE 603 + + QL+ T G K +R WK +NI I NI +W++V +S M W ++ E Sbjct: 285 RTISQLLEGTDGPDKPTVRDCWKAYNIRDAINNIKSSWDDVKQSTMNCAWNAIWKE 340 HSP 2 Score: 36.965 bits (84), Expect = 3.267e-9 Identity = 31/114 (27.19%), Postives = 55/114 (48.25%), Query Frame = -1 Query: 38 GIEGVVEDDIHTLLKSYGEPLTNEELQEIDCHTPLENDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFIQRR 379 G + EDD+ LL SY E LT ++L I ++ + V+++T K + LS + + + D R KR N+++ Y+++ K+R AKQ + F++ R Sbjct: 369 GFSDMREDDVLELLDSYNEELTVDDLMSIAAAD-EAEEQEVVLNVKDMTSKKLTYFLSQADALAQTAMDMDPSLERSLQFKRALNSIIAPYKEVHAQKQR-AKQCTMFQFLRLR 480
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000004972.1 (pep scaffold:Pmarinus_7.0:GL476545:74372:75575:-1 gene:ENSPMAG00000004517.1 transcript:ENSPMAT00000004972.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 53.1434 bits (126), Expect = 1.699e-8 Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = -2 Query: 442 LINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLF 588 L++E + +R+ WK+ N++ IRNI +AW EVTK CM G+WK L Sbjct: 312 LLSEKHATEEKELRMIWKESNVLNAIRNIGRAWKEVTKECMNGIWKNLL 360
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000003283.1 (pep scaffold:Pmarinus_7.0:GL479064:1419:3178:1 gene:ENSPMAG00000003000.1 transcript:ENSPMAT00000003283.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 39.6614 bits (91), Expect = 2.847e-8 Identity = 25/83 (30.12%), Postives = 40/83 (48.19%), Query Frame = -2 Query: 340 LINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEITEIHSYYENNIVV*PFPEASKVLLKMIFILC 588 LI T G A + W+ FNI + I I +AW ++T+ C+ GV ++L + + ++ EA + LK I I C Sbjct: 325 LIEATDGENSQAS-VTWRSFNIKQAIDIIVEAWTDITQPCLNGVCRKLPHVVRDFRAF-----------EAQQEQLKKIQIQC 395 HSP 2 Score: 34.2686 bits (77), Expect = 2.847e-8 Identity = 29/102 (28.43%), Postives = 48/102 (47.06%), Query Frame = -1 Query: 47 SYGEPLTNEELQEIDCHT------PLENDESEPIPVRNLTVKTIEKALSLHNQCVELLEENDEDY*RVNAVKRDCNALLDGYRQILRNKRREAKQGDLDSFI 334 S+ E L+ EELQ++ ++ + E P R LT + AL + ++ LE+ND + R + A L Y ++L KR+ KQ +D F+ Sbjct: 413 SHREELSTEELQKLTAEVVAAIEEDDDDGDPEEAPPRELTAAILSSALQDFERGMQKLEDNDCNAKRSAKILPAIKAQLAPYVELLEEKRKATKQRKIDQFL 514
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000005921.1 (pep scaffold:Pmarinus_7.0:GL487978:976:2666:1 gene:ENSPMAG00000005352.1 transcript:ENSPMAT00000005921.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 47.7506 bits (112), Expect = 1.164e-6 Identity = 17/57 (29.82%), Postives = 36/57 (63.16%), Query Frame = -2 Query: 433 KKLHQLINETGGGGKDAMRLWWKQFNIMKDIRNIDKAWNEVTKSCMKGVWKRLFGEI 603 + L Q + T G +R +W+ +++ + ++NI KAW EVT++C+ G W+++ ++ Sbjct: 296 RTLAQALEATEGDTGKTVREFWEAYDVYQAVQNIAKAWAEVTQTCLNGAWEKMCPQL 352 The following BLAST results are available for this feature:
BLAST of SMED30010448 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30010448 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30010448 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of SMED30010448 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 5
BLAST of SMED30010448 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30010448 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30010448 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 0
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010448 ID=SMED30010448|Name=SMED30010448|organism=Schmidtea mediterranea sexual|type=transcript|length=775bpback to top protein sequence of SMED30010448-orf-1 >SMED30010448-orf-1 ID=SMED30010448-orf-1|Name=SMED30010448-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=106bp MILTKYEYHLQQHLRCLRKWLDDNIIFIIAVNFSNFTEQSFPNTLHTRLGback to top Annotated Terms
The following terms have been associated with this transcript:
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