DNA primase

Overview
NameDNA primase
Smed IDSMED30010424
Length (bp)1202
Neoblast Clusters

Zeng et. al., 2018




▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 



 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)




Explore this single cell expression dataset with our NB Cluster Shiny App




 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of DNA primase (SMED30010424) t-SNE clustered cells

Violin plots show distribution of expression levels for DNA primase (SMED30010424) in cells (dots) of each of the 12 neoblast clusters.

 

back to top


 

Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of DNA primase (SMED30010424) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for DNA primase (SMED30010424) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

back to top


 

Embryonic Expression

Davies et. al., 2017




Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

back to top
Anatomical Expression

PAGE et. al., 2020




SMED30010424

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 7

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010424h1SMcG0019440 SmedASXL_000760SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015
FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30010424h1SMcG0019440 dd_Smed_v4_11558_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
body wall musculatureSMED30010424h1SMcG0019440 dd_Smed_v4_11558_0_1dd_Smed_v4PMID:30471994
Scimone et al., 2018
FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
zeta neoblastSMED30010424h1SMcG0019440 dd_Smed_v4_11558_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017
whole organism asexual adult single-cell RNA-sequencing evidence
neoblastSMED30010424h1SMcG0019440 SMED_29050_V2_1GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30010424h1SMcG0019440 SMED_29050_V2_1GPL14150PMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
neoblastSMED30010424h1SMcG0019440 SmWIOct06_036497GPL14150_gene_modelsPMID:22385657
Wagner et al., 2012
whole organism asexual adult cDNA to DNA expression microarray evidence
Note: Hover over icons to view figure legend
Homology
BLAST of DNA primase vs. Ensembl Human
Match: PRIM1 (DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC:9369])

HSP 1 Score: 354.369 bits (908), Expect = 2.989e-119
Identity = 189/405 (46.67%), Postives = 251/405 (61.98%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSL-VCQLEYENSTKVID-------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS--------------------TQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP S + RWL+YG      F +REFSFTLKDDIY+RY SF ++++  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    ++K     +HP I +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++  +     S     L +W  L      Y+N+ K          EI +++ +PRLD+NVS G+NHLLKSPFSVHP TGRI VPID+  VD+FDPF VPT+     EL +                    T+D   TS   Y+ +FE F+ +L
Sbjct:   11 ELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEK-----IHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETI---HDELQQSFQKSHNS-----LQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENL 402          
BLAST of DNA primase vs. Ensembl Human
Match: PRIM1 (DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC:9369])

HSP 1 Score: 335.88 bits (860), Expect = 1.537e-111
Identity = 189/444 (42.57%), Postives = 251/444 (56.53%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSL-VCQLEYENSTKVID-------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPS---------------------------------------TGRICVPIDIGSVDEFDPFNVPTLEQTISELRS--------------------TQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP S + RWL+YG      F +REFSFTLKDDIY+RY SF ++++  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    ++K     +HP I +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++  +     S     L +W  L      Y+N+ K          EI +++ +PRLD+NVS G+NHLLKSPFSVHP                                        TGRI VPID+  VD+FDPF VPT+     EL +                    T+D   TS   Y+ +FE F+ +L
Sbjct:   11 ELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEK-----IHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETI---HDELQQSFQKSHNS-----LQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTESHSVTQAGVQWRDLGSLQPLPSRFKQFSASPSQVAGITGRISVPIDLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENL 441          
BLAST of DNA primase vs. Ensembl Human
Match: PRIM1 (DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC:9369])

HSP 1 Score: 304.679 bits (779), Expect = 3.736e-101
Identity = 160/330 (48.48%), Postives = 214/330 (64.85%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGNDEA-----FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSL-VCQLEYENSTKVID-------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVH 1014
            EL+K++Y+ +FP S + RWL+YG  +      F +REFSFTLKDDIY+RY SF ++++  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    ++K     +HP I +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++  +     S     L +W  L      Y+N+ K          EI +++ +PRLD+NVS G+NHLLKSPFSVH
Sbjct:   11 ELLKLYYRRLFPYSQYYRWLNYGGGDGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEK-----IHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETI---HDELQQSFQKSHNS-----LQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVH 327          
BLAST of DNA primase vs. Ensembl Human
Match: PRIM1 (DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC:9369])

HSP 1 Score: 170.629 bits (431), Expect = 6.287e-51
Identity = 80/137 (58.39%), Postives = 102/137 (74.45%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALR 468
            EL+K++Y+ +FP S + RWL+YG      F +REFSFTLKDDIY+RY SF ++++  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI++IDRAL+
Sbjct:   11 ELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALK 147          
BLAST of DNA primase vs. Ensembl Human
Match: PRIM1 (DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC:9369])

HSP 1 Score: 105.531 bits (262), Expect = 1.188e-26
Identity = 57/154 (37.01%), Postives = 89/154 (57.79%), Query Frame = 1
Query:  682 ESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECC----------EYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL 1113
            +SI IIK +FE+Y    Q+ L  ++  +++L+++P++            E+   +  ++ N+ ++D     ++W                 EI +++ +PRLD+NVS G+NHLLKSPFSVHP TGRI VPID+  VD+FDPF VPT+     EL
Sbjct:    2 KSINIIKKYFEEYALVNQDILENKESWDKILALVPEKSFQKSHNSLQRWEHLKKVASRYQNNIKNDKYGPWLEW-----------------EIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICREL 138          
BLAST of DNA primase vs. Ensembl Celegans
Match: pri-1 (DNA primase small subunit [Source:UniProtKB/Swiss-Prot;Acc:P34471])

HSP 1 Score: 237.269 bits (604), Expect = 2.778e-74
Identity = 144/377 (38.20%), Postives = 204/377 (54.11%), Query Frame = 1
Query:   37 MSEISVHHDELIKIFYKTIFPSSLFCRWLSYG--NDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPH--FEK------------YLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS 1119
            MS  S+   + + I+YK  FP   F +WL YG  N + F  REF+F L DD+++RY S+ DE+ F + L   NP K+DIGAVYN EP N+K+ +    V   +ELVFDID+TDYD VR CC+ A +C KCW  M +A+K++D  L + F F+  +WV+SGRRGVHCWV DK AR L    RSAI   L   K   K  +  +   +  +  + P + ++  +      FEK            ++T Y+     E G + L S+             ++ L DD  D  + L     L+           +    ++  YPRLDVNVSTG NHLLKSPF +HP TG + VP+++G ++EFD    P ++  + EL S
Sbjct:    1 MSYNSIRLPQDLPIYYKNFFPVKPFTKWLRYGQNNGDYFNRREFAFILADDVHIRYRSYNDEHAFFKALSSTNPEKLDIGAVYNHEPINNKRHTDYQAVE--RELVFDIDLTDYDPVRNCCKDATVCPKCWKFMVLAVKILDFQLEDMFDFKARMWVFSGRRGVHCWVGDKKARMLNNYQRSAIATRLNLFK---KNGQCEVTEGRAKMTKVPPIVRDAFNVALKDGVFEKMIYDQGWLDKEDFITEYK--FMSEVGRDDLRSLSETYKTPQERWNMIRGLFDD--DYRKSLSPKDGLLDFKMQGRDRNYLLYFVLQRCYPRLDVNVSTGTNHLLKSPFCIHPKTGNVAVPLNVGKIEEFDVSKCPRIDHVVEELSS 368          
BLAST of DNA primase vs. Ensembl Celegans
Match: T24C4.5 (DNA primase [Source:UniProtKB/TrEMBL;Acc:Q9TZ34])

HSP 1 Score: 184.496 bits (467), Expect = 5.692e-55
Identity = 93/196 (47.45%), Postives = 126/196 (64.29%), Query Frame = 1
Query:   37 MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGND--EAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNK 618
            MS  S+   + + I+YK  FP   F +WL YG +  + F  REF+F L DD+++RY S+ DE+ F++ L   NP K+DIGAVYN EP N+K+ +    V   +ELVFDID+TDYD VR CC+ A +C KCW  M +A++++D  L E F F+  +WV+SGRRGVHCWV DK AR L  + RSAI   L   K   +
Sbjct:    1 MSYNSIRLPQDLPIYYKNFFPVKPFTKWLCYGQNYGDYFNRREFTFILADDVHIRYRSYNDEHSFVKALSSTNPEKLDIGAVYNHEPINNKRYTDYQAVE--RELVFDIDLTDYDPVRNCCKDATVCPKCWKFMVLAVRILDFQLEEMFNFKARMWVFSGRRGVHCWVGDKKARMLNNNQRSAIATRLNLFKKNGQ 194          
BLAST of DNA primase vs. Ensembl Celegans
Match: T24C4.5 (DNA primase [Source:UniProtKB/TrEMBL;Acc:Q9TZ34])

HSP 1 Score: 184.496 bits (467), Expect = 5.692e-55
Identity = 93/196 (47.45%), Postives = 126/196 (64.29%), Query Frame = 1
Query:   37 MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGND--EAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNK 618
            MS  S+   + + I+YK  FP   F +WL YG +  + F  REF+F L DD+++RY S+ DE+ F++ L   NP K+DIGAVYN EP N+K+ +    V   +ELVFDID+TDYD VR CC+ A +C KCW  M +A++++D  L E F F+  +WV+SGRRGVHCWV DK AR L  + RSAI   L   K   +
Sbjct:    1 MSYNSIRLPQDLPIYYKNFFPVKPFTKWLCYGQNYGDYFNRREFTFILADDVHIRYRSYNDEHSFVKALSSTNPEKLDIGAVYNHEPINNKRYTDYQAVE--RELVFDIDLTDYDPVRNCCKDATVCPKCWKFMVLAVRILDFQLEEMFNFKARMWVFSGRRGVHCWVGDKKARMLNNNQRSAIATRLNLFKKNGQ 194          
BLAST of DNA primase vs. Ensembl Celegans
Match: pri-1 (DNA primase small subunit [Source:UniProtKB/Swiss-Prot;Acc:P34471])

HSP 1 Score: 80.1073 bits (196), Expect = 3.701e-18
Identity = 33/58 (56.90%), Postives = 43/58 (74.14%), Query Frame = 1
Query:  946 YPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS 1119
            YPRLDVNVSTG NHLLKSPF +HP TG + VP+++G ++EFD    P ++  + EL S
Sbjct:   18 YPRLDVNVSTGTNHLLKSPFCIHPKTGNVAVPLNVGKIEEFDVSKCPRIDHVVEELSS 75          
BLAST of DNA primase vs. Ensembl Celegans
Match: pri-1 (DNA primase small subunit [Source:UniProtKB/Swiss-Prot;Acc:P34471])

HSP 1 Score: 80.1073 bits (196), Expect = 3.701e-18
Identity = 33/58 (56.90%), Postives = 43/58 (74.14%), Query Frame = 1
Query:  946 YPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS 1119
            YPRLDVNVSTG NHLLKSPF +HP TG + VP+++G ++EFD    P ++  + EL S
Sbjct:   18 YPRLDVNVSTGTNHLLKSPFCIHPKTGNVAVPLNVGKIEEFDVSKCPRIDHVVEELSS 75          
BLAST of DNA primase vs. Ensembl Fly
Match: DNApol-alpha50 (gene:FBgn0011762 transcript:FBtr0076603)

HSP 1 Score: 307.375 bits (786), Expect = 4.885e-101
Identity = 161/412 (39.08%), Postives = 237/412 (57.52%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYV-----KGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTK-------------VIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS-----------------TQDIHSTSFGKYLDIFERFINDL 1188
            +++ ++Y+ +FP   F RWLSYG+  D  F NRE SFTL+DDIY+RYL F  + E  +E+  +NP KIDIG V + +PKNH+    G   P  +ELVFDIDMTDYD+VR CC  A +C KCW  M +A +V+D ALREDFGFE I+W++SGRRG+HCWVCD  AR L    R A+  YL  +      GGN  +      D+      H  +  +++I++P FE+ +   QN     KG+ +LL+++ D+    R  ++  ++  +  D     + W S +   +Y NS +             ++ EI +  +YPRLD+NV+ G NHLLK+PF +HP+TG++CVP  + +V +FDP  VPT+ Q + E+ +                  +D   TS  K + +FE F+  L
Sbjct:   30 DMLPVYYRRLFPHLPFYRWLSYGSSEDAIFSNREISFTLQDDIYIRYLCFDTQAELEKEICSRNPIKIDIGPVMHSKPKNHR-SIPGGLTPVQRELVFDIDMTDYDEVRTCCSGAGVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAVAEYLNIITYASFAGGNSPR--CSMGDRP-----HHSLKRALKIVEPMFEEIVLEDQNLFGTPKGVTKLLNMVHDDAA--RGELE-SYMQKNLEDGAHSRLVWESFI---KYANSMRTSTTSAWSRKLKNIVAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPATGKVCVPFSVSAVAKFDPTTVPTITQLLHEINAFDDKSKSYMEAPEDKSRIKDHKKTSMFKGVVVFEEFLRKL 427          
BLAST of DNA primase vs. Ensembl Zebrafish
Match: prim1 (DNA primase subunit 1 [Source:ZFIN;Acc:ZDB-GENE-990603-6])

HSP 1 Score: 358.992 bits (920), Expect = 2.989e-121
Identity = 184/408 (45.10%), Postives = 253/408 (62.01%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYG--NDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTK------VIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTL-------------------------EQTISELRSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP S + RWLSYG  +   F NREFSFTLKDDIYVRY SF  +NE  +E+ +  PYKIDIGAVY+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI+++DRALREDFGF  +LWVYSGRRGVHCWVCD  AR L   ARSA+  YL+ VKGG +  +  + +D      +HP I +S+ +++ +F +Y    Q+ L  ++ ++++L+++P++          K L +   +  + + +W  L   ++++ S+          EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VPID+  +D FDPF VPT+                         EQ  +E R  +D   TS  KY+ + +RF++ +
Sbjct:   13 DLLPLYYRRLFPFSQYFRWLSYGGVSKNYFQNREFSFTLKDDIYVRYQSFSSQNELEKEMQKMVPYKIDIGAVYSHRPSQHNTVKSGTFQALEKELVFDIDMTDYDDVRRCCSAADICSKCWTLMTIAIRILDRALREDFGFHHLLWVYSGRRGVHCWVCDDAARKLSVAARSAVAEYLSLVKGGEETLRKVVLSDP-----IHPFISQSLSVVEHYFPQYAIVDQDILGNKESVDKVLALLPEDI--------KKDLIEYYQNHKEPMKRWDRLRQLVDHKKSSSKKGGQYYDKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLKELDTFDPFEVPTISLICRELEKPRADEAEDEDVKDKENEQEAAERRRIRDYKRTSLAKYVKVLDRFLDSM 407          
BLAST of DNA primase vs. Ensembl Xenopus
Match: rhagl (Rh associated glycoprotein like [Source:Xenbase;Acc:XB-GENE-5797751])

HSP 1 Score: 340.502 bits (872), Expect = 3.583e-114
Identity = 181/399 (45.36%), Postives = 253/399 (63.41%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKK-KKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL---------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP   + RWL+YG      F +REFSFTLKDD+YVRY SF +++E  +E+ +  PYKIDIGAVY+ +P  H    +G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A++++DRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L    RSA+  YL+ VKGG +  KKV L +       +HP I +S+++++ +FEKY    Q+ L  ++  +++++++P++       + L +L        + +V+     C L++     +  EI ++F YPRLDVNVS G+NHLLKSPFSVHP TGRI VPID   +D+FDPF+VPT+    SEL                     + T+D   TS   Y+ +FE+F++ L
Sbjct:   13 DLLPLYYRRLFPFYQYFRWLNYGGVVKNYFHHREFSFTLKDDVYVRYQSFNNQSELEKEMQKMCPYKIDIGAVYSHKPSLHNTVKSGTFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTIAVRILDRALAEDFGFQHRLWVYSGRRGVHCWVCDDSARKLSQAERSAVAEYLSVVKGGEETIKKVQLSD------PIHPFIGKSLKMVERYFEKYALVDQDILENKQCWDKVVALVPED-------ILLDFLQ-------RLMVQVEIWSCLLDF----NIPKEIMLQFCYPRLDVNVSKGLNHLLKSPFSVHPKTGRISVPIDTKKLDQFDPFSVPTISLICSELDNVCKKEDDQDSSGEGEPEAKKRTRDYKRTSLAPYIKVFEQFLDKL 387          
BLAST of DNA primase vs. Ensembl Xenopus
Match: rhagl (Rh associated glycoprotein like [Source:Xenbase;Acc:XB-GENE-5797751])

HSP 1 Score: 340.502 bits (872), Expect = 3.993e-114
Identity = 181/399 (45.36%), Postives = 253/399 (63.41%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKK-KKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL---------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP   + RWL+YG      F +REFSFTLKDD+YVRY SF +++E  +E+ +  PYKIDIGAVY+ +P  H    +G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A++++DRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L    RSA+  YL+ VKGG +  KKV L +       +HP I +S+++++ +FEKY    Q+ L  ++  +++++++P++       + L +L        + +V+     C L++     +  EI ++F YPRLDVNVS G+NHLLKSPFSVHP TGRI VPID   +D+FDPF+VPT+    SEL                     + T+D   TS   Y+ +FE+F++ L
Sbjct:   14 DLLPLYYRRLFPFYQYFRWLNYGGVVKNYFHHREFSFTLKDDVYVRYQSFNNQSELEKEMQKMCPYKIDIGAVYSHKPSLHNTVKSGTFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTIAVRILDRALAEDFGFQHRLWVYSGRRGVHCWVCDDSARKLSQAERSAVAEYLSVVKGGEETIKKVQLSD------PIHPFIGKSLKMVERYFEKYALVDQDILENKQCWDKVVALVPED-------ILLDFLQ-------RLMVQVEIWSCLLDF----NIPKEIMLQFCYPRLDVNVSKGLNHLLKSPFSVHPKTGRISVPIDTKKLDQFDPFSVPTISLICSELDNVCKKEDDQDSSGEGEPEAKKRTRDYKRTSLAPYIKVFEQFLDKL 388          
BLAST of DNA primase vs. Ensembl Xenopus
Match: rhagl (Rh associated glycoprotein like [Source:Xenbase;Acc:XB-GENE-5797751])

HSP 1 Score: 335.495 bits (859), Expect = 2.712e-112
Identity = 177/392 (45.15%), Postives = 246/392 (62.76%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRST---------------QDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP   + RWL+YG      F +REFSFTLKDD+YVRY SF +++E  +E+ +  PYKIDIGAVY+ +P  H    +G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A++++DRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L    RSA+  YL+ VKGG +  K    +D      +HP I +S+++++ +FEKY    Q+ L  ++  +++++++P++       + L +L        + +V +    C +          EI ++F YPRLDVNVS G+NHLLKSPFSVHP TGRI VPID   +D+FDPF+VPT+    SEL +                 D   TS   Y+ +FE+F++ L
Sbjct:   13 DLLPLYYRRLFPFYQYFRWLNYGGVVKNYFHHREFSFTLKDDVYVRYQSFNNQSELEKEMQKMCPYKIDIGAVYSHKPSLHNTVKSGTFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTIAVRILDRALAEDFGFQHRLWVYSGRRGVHCWVCDDSARKLSQAERSAVAEYLSVVKGGEETIKKVQLSDP-----IHPFIGKSLKMVERYFEKYALVDQDILENKQCWDKVVALVPED-------ILLDFLQ-------RLMVIFSVRNCNIP--------KEIMLQFCYPRLDVNVSKGLNHLLKSPFSVHPKTGRISVPIDTKKLDQFDPFSVPTISLICSELDNVCKKEDDQDSSGLCCFSDYKRTSLAPYIKVFEQFLDKL 377          
BLAST of DNA primase vs. Ensembl Xenopus
Match: rhagl (Rh associated glycoprotein like [Source:Xenbase;Acc:XB-GENE-5797751])

HSP 1 Score: 333.954 bits (855), Expect = 3.177e-111
Identity = 172/357 (48.18%), Postives = 233/357 (65.27%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLE-----YENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL 1113
            +L+ ++Y+ +FP   + RWL+YG      F +REFSFTLKDD+YVRY SF +++E  +E+ +  PYKIDIGAVY+ +P  H    +G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A++++DRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L    RSA+  YL+ VKGG +  K    +D      +HP I +S+++++ +FEKY    Q+ L  ++  +++++++P+   E         L  + S     + +W  L   LE         + +  EI ++F YPRLDVNVS G+NHLLKSPFSVHP TGRI VPID   +D+FDPF+VPT+    SEL
Sbjct:   13 DLLPLYYRRLFPFYQYFRWLNYGGVVKNYFHHREFSFTLKDDVYVRYQSFNNQSELEKEMQKMCPYKIDIGAVYSHKPSLHNTVKSGTFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTIAVRILDRALAEDFGFQHRLWVYSGRRGVHCWVCDDSARKLSQAERSAVAEYLSVVKGGEETIKKVQLSDP-----IHPFIGKSLKMVERYFEKYALVDQDILENKQCWDKVVALVPEVAREN--------LLREFSKARSSVERWDKLSSCLEATVKDLRRHSNIPKEIMLQFCYPRLDVNVSKGLNHLLKSPFSVHPKTGRISVPIDTKKLDQFDPFSVPTISLICSEL 356          
BLAST of DNA primase vs. Ensembl Xenopus
Match: rhagl (Rh associated glycoprotein like [Source:Xenbase;Acc:XB-GENE-5797751])

HSP 1 Score: 331.643 bits (849), Expect = 4.949e-111
Identity = 170/357 (47.62%), Postives = 233/357 (65.27%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLE-----YENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL 1113
            +L+ ++Y+ +FP   + RWL+YG      F +REFSFTLKDD+YVRY SF +++E  +E+ +  PYKIDIGAVY+ +P  H    +G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A++++DRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L    RSA+  YL+ VKGG +  K    +D      +HP I +S+++++ +FEKY    Q+ L  ++  +++++++P+   E         L  + S     + +W  L   LE         + +  EI ++F YPRLDVNVS G+NHLLKSPFSVHP TGRI VPID   +D+FDPF+VPT+    ++L
Sbjct:   13 DLLPLYYRRLFPFYQYFRWLNYGGVVKNYFHHREFSFTLKDDVYVRYQSFNNQSELEKEMQKMCPYKIDIGAVYSHKPSLHNTVKSGTFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTIAVRILDRALAEDFGFQHRLWVYSGRRGVHCWVCDDSARKLSQAERSAVAEYLSVVKGGEETIKKVQLSDP-----IHPFIGKSLKMVERYFEKYALVDQDILENKQCWDKVVALVPEVAREN--------LLREFSKARSSVERWDKLSSCLEATVKDLRRHSNIPKEIMLQFCYPRLDVNVSKGLNHLLKSPFSVHPKTGRISVPIDTKKLDQFDPFSVPTIRFATNKL 356          
BLAST of DNA primase vs. Ensembl Mouse
Match: Prim1 (DNA primase, p49 subunit [Source:MGI Symbol;Acc:MGI:97757])

HSP 1 Score: 349.747 bits (896), Expect = 1.262e-117
Identity = 186/405 (45.93%), Postives = 249/405 (61.48%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVID----------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP + + RWL+YG      F +REFSFTLKDDIY+RY SF +++E  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A+++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    N+K     +HP + +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++ +      S P     +W  L    +  NS++ +           E+ +++ +PRLDVNVS GVNHLLKSPFSVHP TGRI VPID   VD+FDPF VPT+     EL                     +    TS   Y+ +FE+F+ +L
Sbjct:   11 ELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHREFSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEK-----VHPFVRKSINIIKKYFEEYALVGQDILENKENWDKILALVPETI---HDELQ-RGFQKFHSSPQ----RWEHL---RKVANSSQNMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENL 399          
BLAST of DNA primase vs. Ensembl Mouse
Match: Prim1 (DNA primase, p49 subunit [Source:MGI Symbol;Acc:MGI:97757])

HSP 1 Score: 348.977 bits (894), Expect = 1.947e-117
Identity = 186/405 (45.93%), Postives = 249/405 (61.48%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVID----------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP + + RWL+YG      F +REFSFTLKDDIY+RY SF +++E  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A+++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    N+K     +HP + +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++ +      S P     +W  L    +  NS++ +           E+ +++ +PRLDVNVS GVNHLLKSPFSVHP TGRI VPID   VD+FDPF VPT+     EL                     +    TS   Y+ +FE+F+ +L
Sbjct:   11 ELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHREFSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEK-----VHPFVRKSINIIKKYFEEYALVGQDILENKENWDKILALVPE--TPIHDELQ-RGFQKFHSSPQ----RWEHL---RKVANSSQNMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENL 400          
BLAST of DNA primase vs. UniProt/SwissProt
Match: sp|P49642|PRI1_HUMAN (DNA primase small subunit OS=Homo sapiens OX=9606 GN=PRIM1 PE=1 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 1.435e-118
Identity = 189/405 (46.67%), Postives = 251/405 (61.98%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSL-VCQLEYENSTKVID-------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS--------------------TQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP S + RWL+YG      F +REFSFTLKDDIY+RY SF ++++  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    ++K     +HP I +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++  +     S     L +W  L      Y+N+ K          EI +++ +PRLD+NVS G+NHLLKSPFSVHP TGRI VPID+  VD+FDPF VPT+     EL +                    T+D   TS   Y+ +FE F+ +L
Sbjct:   11 ELLKLYYRRLFPYSQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICPKCWTLMTMAIRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLSEK-----IHPFIRKSINIIKKYFEEYALVNQDILENKESWDKILALVPETI---HDELQQSFQKSHNS-----LQRWEHLKKVASRYQNNIKNDKYGPWLEWEIMLQYCFPRLDINVSKGINHLLKSPFSVHPKTGRISVPIDLQKVDQFDPFTVPTISFICRELDAISTNEEEKEENEAESDVKHRTRDYKKTSLAPYVKVFEHFLENL 402          
BLAST of DNA primase vs. UniProt/SwissProt
Match: sp|P20664|PRI1_MOUSE (DNA primase small subunit OS=Mus musculus OX=10090 GN=Prim1 PE=1 SV=1)

HSP 1 Score: 349.747 bits (896), Expect = 8.825e-117
Identity = 186/405 (45.93%), Postives = 249/405 (61.48%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVID----------EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            EL+K++Y+ +FP + + RWL+YG      F +REFSFTLKDDIY+RY SF +++E  +E+ + NPYKIDIGAVY+  P  H     G F  + KELVFDIDMTDYDDVR CC  ADIC KCW LMT+A+++IDRAL+EDFGF+  LWVYSGRRGVHCWVCD+  R L    RS IV YL+ VKGG   KK    N+K     +HP + +SI IIK +FE+Y    Q+ L  ++  +++L+++P+      D ++ +      S P     +W  L    +  NS++ +           E+ +++ +PRLDVNVS GVNHLLKSPFSVHP TGRI VPID   VD+FDPF VPT+     EL                     +    TS   Y+ +FE+F+ +L
Sbjct:   11 ELLKLYYRRLFPYAQYYRWLNYGGVTKNYFQHREFSFTLKDDIYIRYQSFNNQSELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKLGAFQAQEKELVFDIDMTDYDDVRRCCSSADICSKCWTLMTMAMRIIDRALKEDFGFKHRLWVYSGRRGVHCWVCDESVRKLSSAVRSGIVEYLSLVKGGQDVKKKVHLNEK-----VHPFVRKSINIIKKYFEEYALVGQDILENKENWDKILALVPETI---HDELQ-RGFQKFHSSPQ----RWEHL---RKVANSSQNMKNDKCGPWLEWEVMLQYCFPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDFHKVDQFDPFTVPTISAICRELDMVSTHEKEKEENEADSKHRVRGYKKTSLAPYVKVFEQFLENL 399          
BLAST of DNA primase vs. UniProt/SwissProt
Match: sp|Q24317|PRI1_DROME (DNA primase small subunit OS=Drosophila melanogaster OX=7227 GN=DNApol-alpha50 PE=1 SV=2)

HSP 1 Score: 307.375 bits (786), Expect = 4.897e-100
Identity = 161/412 (39.08%), Postives = 237/412 (57.52%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYV-----KGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTK-------------VIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRS-----------------TQDIHSTSFGKYLDIFERFINDL 1188
            +++ ++Y+ +FP   F RWLSYG+  D  F NRE SFTL+DDIY+RYL F  + E  +E+  +NP KIDIG V + +PKNH+    G   P  +ELVFDIDMTDYD+VR CC  A +C KCW  M +A +V+D ALREDFGFE I+W++SGRRG+HCWVCD  AR L    R A+  YL  +      GGN  +      D+      H  +  +++I++P FE+ +   QN     KG+ +LL+++ D+    R  ++  ++  +  D     + W S +   +Y NS +             ++ EI +  +YPRLD+NV+ G NHLLK+PF +HP+TG++CVP  + +V +FDP  VPT+ Q + E+ +                  +D   TS  K + +FE F+  L
Sbjct:   30 DMLPVYYRRLFPHLPFYRWLSYGSSEDAIFSNREISFTLQDDIYIRYLCFDTQAELEKEICSRNPIKIDIGPVMHSKPKNHR-SIPGGLTPVQRELVFDIDMTDYDEVRTCCSGAGVCLKCWKFMVLAARVLDVALREDFGFEHIIWIFSGRRGIHCWVCDYQARHLDGRGRYAVAEYLNIITYASFAGGNSPR--CSMGDRP-----HHSLKRALKIVEPMFEEIVLEDQNLFGTPKGVTKLLNMVHDDAA--RGELE-SYMQKNLEDGAHSRLVWESFI---KYANSMRTSTTSAWSRKLKNIVAEIQLGLLYPRLDINVTRGFNHLLKAPFCIHPATGKVCVPFSVSAVAKFDPTTVPTITQLLHEINAFDDKSKSYMEAPEDKSRIKDHKKTSMFKGVVVFEEFLRKL 427          
BLAST of DNA primase vs. UniProt/SwissProt
Match: sp|O14215|PRI1_SCHPO (DNA primase small subunit OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=pri1 PE=1 SV=1)

HSP 1 Score: 271.552 bits (693), Expect = 8.469e-86
Identity = 154/362 (42.54%), Postives = 213/362 (58.84%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYG----NDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYEN----STKVI----DEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL 1113
            E +  +Y+ +FP     +WL++G    ND  F +REF+FTL +D Y+RYLSF +  E  +E +   P + ++G VY+  P++ K      F P  KELVFDIDMTDYDDVR CC K +IC+KCWP +T+A++V+D    EDFGF+ ILWVYSGRRG+H W+CD+ A  L   +R  I  YL  V  GN +  V L N+ K  R LHPH+  S+ I+K  F K +   Q+    ++G   LL ++     +   A++ KW  D +        KW  +   L   +    S  VI     +I + ++YPRLDV VS  +NHLLKSPF VHP T R+CVPIDI  +D F+P  VPT+   + EL
Sbjct:   33 ETMIQYYRHLFPWKYLFQWLNHGPVVTND--FAHREFAFTLPNDAYIRYLSFSNWEELKKEALNLCPSRFEVGPVYSANPRDRKTIRKSTFHPLKKELVFDIDMTDYDDVRTCCSKTNICEKCWPFITIAVQVLDICFHEDFGFKHILWVYSGRRGIHAWICDEIACSLDDRSRRMIASYLQVVV-GNPQGGVRLINNLK--RPLHPHLTRSLNILKSAFVKIVLEDQDPWASKEGAENLLKLL--PDKDLASALRKKWEVDPERSSKN---KWSDIDTVLASGSIASISPSVIAIAKQDIVLTYLYPRLDVEVSRHLNHLLKSPFCVHPGTSRVCVPIDIERMDSFNPLKVPTVNDLLQEL 384          
BLAST of DNA primase vs. UniProt/SwissProt
Match: sp|Q25998|PRI1_PLAFK (DNA primase small subunit OS=Plasmodium falciparum (isolate K1 / Thailand) OX=5839 PE=3 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 2.454e-82
Identity = 158/417 (37.89%), Postives = 229/417 (54.92%), Query Frame = 1
Query:   52 VHHDELIKIFYKTIFPSSLFCRWLSYGND-----------EAFCNREFSFTLK------DDIYVRYLSFKDENEFIEELIRK-NPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGN-KKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRD---AMKL----KWLNDDQSDPTQCLVKWRSLVCQLEYENSTK------------VIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRSTQDIH-----STSFGKYLDIFER 1173
            V+ ++LI  +Y+++ P +    WL+Y ND             F  REFSFT K      ++IY+R+LSF +  EF  +L+    P K DIGA+YN  P + K      F+P  KEL+FDIDM DYDD+R CC    +CK CW  +TVAI ++D ALREDF FE ILWVYSGRRG+HCWV D+  R    DAR+A+  YL  + G + KKKKV++     W ++ +P    + +I   +F+  +            + +L+  +P    +  D   AMKL    +++N++  +  +   K+ S+   L   N  K             + EI   F YPRLD+NVS  +NHLLKSPF +H STGR+CVP+DI +++ F+P +VPTL+    +    ++ H      TS   Y+D F R
Sbjct:   14 VNENDLI-FYYRSLCPINDLYNWLNYKNDIKGKYTKLNDPHFFSKREFSFTCKKSDQGKEEIYIRWLSFSNPEEFKNKLLSDLVPIKFDIGAIYNF-PVSQKDQKGDIFLPVQKELIFDIDMNDYDDIRTCCTDKKVCKLCWKFLTVAIVLLDTALREDFSFEHILWVYSGRRGIHCWVADESCRYYTTDARAALADYLNILSGSDTKKKKVSI-----WGKDKYPMFERAFDICYKYFDVLMEEQDFFKKGSPHVQKLIDYLPYASGKVTDPLKAMKLNELKEYINNNNFNSREIFEKFSSIYNFLTPSNYFKRKNVSGNINMPSFVKEIVFHFTYPRLDINVSKEINHLLKSPFCIHNSTGRVCVPLDIKNINNFNPQSVPTLKLLREQFDDPKNSHIEAENRTSLKPYIDYFRR 423          
BLAST of DNA primase vs. TrEMBL
Match: A0A210Q007 (DNA primase OS=Mizuhopecten yessoensis OX=6573 GN=KP79_PYT24397 PE=3 SV=1)

HSP 1 Score: 372.089 bits (954), Expect = 3.369e-123
Identity = 189/392 (48.21%), Postives = 258/392 (65.82%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKK-KKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVC--QLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRST------------QDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP   + RWL+YG      F NREFSFTLKDD+Y+RY SF D+ E  +E+ ++ PYKIDIGAV++  PK+HK      F  + KELVFDIDMTDYDDVR CC  ADIC  CWPLM +AIK+IDRALR+DFGF+ +LWVYSGRRGVHCWVCD+ AR L    R+AI  YL+ VKGG ++ KKVTL +       +HP I +SI+I++ HF  Y    Q+ L  E+   ++L+++PD+    R  ++  +     S     +++ R   C  +  ++ S  +++EI +++ YPRLDVNVS GVNHLLKSPF +HP TGR+CVPIDI  V++FDP  VPT+ Q + EL  T            +D   TS G+YL IF++F+  L
Sbjct:   16 DLLPMYYRKLFPYGPYFRWLNYGGAPKSYFTNREFSFTLKDDVYIRYQSFSDKQEMEKEIQKRCPYKIDIGAVFSHRPKDHKTVKAAAFQAQEKELVFDIDMTDYDDVRSCCSGADICNLCWPLMQIAIKIIDRALRDDFGFKHMLWVYSGRRGVHCWVCDEVARKLSAQGRTAIAEYLSVVKGGEQQTKKVTLDD------PIHPSIRQSIDIVRQHFHDYSIKKQDFLGDEEKQKKVLALVPDDVL--RKTLEGDFEMGSNSGNWWAIIEKREKDCVSKGSFKASHHIVEEIMLQYCYPRLDVNVSKGVNHLLKSPFCIHPKTGRVCVPIDIEHVEDFDPMAVPTISQILDELNQTSAAAADDSGKKVKDYKKTSMGEYLRIFDKFLKGL 399          
BLAST of DNA primase vs. TrEMBL
Match: A0A1S3HJ03 (DNA primase OS=Lingula unguis OX=7574 GN=LOC106155693 PE=3 SV=1)

HSP 1 Score: 370.548 bits (950), Expect = 1.596e-122
Identity = 188/404 (46.53%), Postives = 260/404 (64.36%), Query Frame = 1
Query:   37 MSEISVHHD-----ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGG-NKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            M+    H+D     + + I+YK +FP  L+ +WL+YG      F NREFSFTLKDD+YVRY SF D+ +  +E+ ++ PYKIDIGAV+   PK+HK      F  + KELVFDIDMTDYD+VR CC  ADIC KCWPLM +A+K+ID ALREDFGFE +LWVYSGRRG+HCWVCD+ AR L  +AR+AI  YL+ VKGG N+ KK TLY+D     ++HP +  +I+I++ HF+ Y    Q+    E+   ++L+++PD+    +     K +  + S     ++K R    + + + S  V +EI +++ YPRLDVNVS G+NHLLKSPF VHP TGR+CV +D   +D F+PF VPTL Q   EL            +  +D   TS  +Y +IF++F++ L
Sbjct:    1 MTNEQTHYDPACLPDFLPIYYKRLFPYGLYYQWLNYGGVPKTYFTNREFSFTLKDDVYVRYQSFADQQDMEKEIQKRCPYKIDIGAVFTHRPKDHKTVKAAAFQAQEKELVFDIDMTDYDEVRSCCSGADICPKCWPLMIIAVKIIDTALREDFGFEHLLWVYSGRRGIHCWVCDEQARKLSQNARTAIAEYLSVVKGGENQTKKCTLYDD-----SIHPSVRRAIDIVEDHFDDYAIRKQDFAGSEEKWGKVLALVPDDAVRSQVLEDCKEVKAESSRARWAILKKRQQDFKDKCKKSWCVTEEIMLQYCYPRLDVNVSKGINHLLKSPFCVHPKTGRVCVSMDPKKIDSFNPFKVPTLSQLCDELNQLENKSEDDPKKRVKDYKRTSLKEYAEIFDKFVHKL 399          
BLAST of DNA primase vs. TrEMBL
Match: B3RZA4 (DNA primase OS=Trichoplax adhaerens OX=10228 GN=TRIADDRAFT_26575 PE=3 SV=1)

HSP 1 Score: 368.237 bits (944), Expect = 6.331e-122
Identity = 189/380 (49.74%), Postives = 248/380 (65.26%), Query Frame = 1
Query:   34 NMSEISVHHD-----ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIP------DECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYE-----NS--TKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL 1113
            N S +SV +D     +L+ ++YK +FP  L+ +WLSYGN     F NREFSFTLKDDIYVRYLSF D+NE  +E+ +++PYKID+GAVY+  PK+        F PE KELVFDIDMTDYD++R CC  A+IC KCWP MTVA+K++D ALREDFGF  +LWVYSGRRG+HCWVCD  AR L   ARSAI  YLT +KGG    K   + ++ +   LHP I  +++ I+ +FE  L   QN L  E+  N++L++IP      D+   Y  ++  +W N   S     L KW  L  ++E +     NS  T  + EI +++ YPRLD+NVS G+NHLLKSPF +HP TGR+CVPI    +D FDPF+VP +    SEL
Sbjct:    7 NTSTLSVKYDLTDLPQLMAVYYKRLFPYQLYYKWLSYGNVSKNYFANREFSFTLKDDIYVRYLSFSDKNEMQKEIQKRSPYKIDLGAVYSFRPKDRNMIKNSSFQPEEKELVFDIDMTDYDEIRSCCSGAEICSKCWPFMTVAMKILDAALREDFGFHHLLWVYSGRRGIHCWVCDAAARKLSQSARSAIAEYLTIIKGGEGHVKRVHFKNQAY---LHPSIQRALKTIESYFEDLLLKKQNILESEESSNKVLALIPDKNILKDQIRFYFFSLSEEWNNSGLSS----LQKWSLLKQKIEKDIVSTKNSVLTHCVAEIQLQYTYPRLDINVSKGINHLLKSPFCIHPKTGRVCVPIPATDIDNFDPFSVPNICHLCSEL 379          
BLAST of DNA primase vs. TrEMBL
Match: A0A3B4VJW6 (DNA primase OS=Seriola dumerili OX=41447 PE=3 SV=1)

HSP 1 Score: 369.007 bits (946), Expect = 7.561e-122
Identity = 185/401 (46.13%), Postives = 254/401 (63.34%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTL------------------------EQTISELRSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP S + RWL+YG  +   F NREFSFTLKDDIYVRY SF  +NE  +E+ + NPYKIDIGA+Y+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI ++DRALREDFGF+ +LWVYSGRRGVHCWVCD+ AR L   ARSAI  YL+ VKGG +  K  +  D      +HP I ES+ +++ +F +Y    Q  L +++ ++++L+++P++    R  ++  + N+ + +    LVK ++   Q   +       EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VP+D+  +D FDPF VPT+                        E+  +E R  +D   TS  KY+  F++F++ +
Sbjct:   13 DLLPLYYRRLFPFSQYYRWLNYGGVQKNYFQNREFSFTLKDDIYVRYQSFTTQNELEKEMQKMNPYKIDIGAIYSHRPNQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICSKCWTLMTIAIHILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAIAEYLSLVKGGEETVKKVVLTDP-----IHPSIRESLSVVERYFSQYALHEQEILGRKESVDKVLALVPEDV---RKDLQQSFQNEKKPETRWKLVKDQATRKQATAKKGQHFEKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPMDLKELDTFDPFAVPTISMICEELDRPRTEEESEDTKEKENEKDAAEKRKIRDYKRTSLAKYVKYFDQFLDGM 405          
BLAST of DNA primase vs. TrEMBL
Match: A0A556U2J1 (DNA primase OS=Bagarius yarrelli OX=175774 GN=Baya_7786 PE=3 SV=1)

HSP 1 Score: 369.392 bits (947), Expect = 2.440e-121
Identity = 190/407 (46.68%), Postives = 260/407 (63.88%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSL---VCQLEYENSTKVID----EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTL-----------------------EQTISELRSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP S + RWLSYG      F NREFSFTLKDDIYVRY SF  ++E  +E+ + NPYKIDIGAVY+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI+++DRALR+DFGF+ +LWVYSGRRGVHCWVCD+ AR L   ARSA+  YL+ VKGG +  K  + +D      +HP I +S+ +++ +F +Y    Q+ L+ +  ++++LS++PD+   Y+ ++K + LN  Q++    + +W  L     Q +  +S K       EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VPID+ ++D+FDPF VPT+                       E    E R  +D   TS  KY+ +F+ F++ +
Sbjct:   13 DLLPLYYRRLFPYSQYFRWLSYGGASKNYFQNREFSFTLKDDIYVRYQSFSTQSELEKEMQKMNPYKIDIGAVYSHRPSQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICSKCWTLMTIAIRILDRALRDDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLVKGGEETVKKVVLSDP-----IHPFISQSLIVVEHYFPQYAVVEQDLLSSKASVDKVLSLVPDDILHYKISVK-ELLNIYQTEKNP-MKRWEQLCRYAVQQKRTSSKKGAQYFDKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLKNLDKFDPFEVPTISLICEELDKPLTAEEEDVKDKESETESGERRKIRDYKHTSLAKYVKLFDHFLDAM 412          
BLAST of DNA primase vs. Ensembl Cavefish
Match: prim1 (DNA primase subunit 1 [Source:NCBI gene;Acc:103027008])

HSP 1 Score: 367.081 bits (941), Expect = 1.703e-124
Identity = 189/405 (46.67%), Postives = 260/405 (64.20%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQ--LEYENSTKVID-EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL-------------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP S + RWLSYG      F NREFSFTLKDDIYVRY SF  +NE  +E+ + NPYKIDIGAVY+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI+++DRALREDFGF+ +LWVYSGRRGVHCWVCD+ AR L   ARSA+  YL+ VKGG +  K  + +D      +HP I +S+ +++ +F +Y    Q  L+ ++G++++L+++P++    R  + L +    + +PT+   + ++LV Q     +   +  D EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VPID+ ++D+FDPF VPT+     EL                         R  +D   TS  KY+ +F++F++ +
Sbjct:   13 DLLPLYYRRLFPFSQYYRWLSYGGVTKNYFQNREFSFTLKDDIYVRYQSFSTQNELEKEMQKMNPYKIDIGAVYSHRPSQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICSKCWTLMTIAIRILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLVKGGEETVKKVVLSDP-----IHPFISQSLSVVEHYFLQYAIVGQELLSSKEGVDKVLNLLPEDV---RKDLLLHY--QSEKNPTKRWERLKTLVHQKRATSKKGGQYFDKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLRNLDKFDPFEVPTISLICEELDKPRPDEEEEDDTKDKENEIEAGERRKIRDFKRTSLAKYIKLFDQFLDSV 407          
BLAST of DNA primase vs. Ensembl Cavefish
Match: prim1 (DNA primase subunit 1 [Source:NCBI gene;Acc:103027008])

HSP 1 Score: 347.821 bits (891), Expect = 1.933e-117
Identity = 178/376 (47.34%), Postives = 242/376 (64.36%), Query Frame = 1
Query:  145 FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQ--LEYENSTKVID-EIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL-------------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            F NREFSFTLKDDIYVRY SF  +NE  +E+ + NPYKIDIGAVY+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI+++DRALREDFGF+ +LWVYSGRRGVHCWVCD+ AR L   ARSA+  YL+ VKGG +  K  + +D      +HP I +S+ +++ +F +Y    Q  L+ ++G++++L+++P++    R  + L +    + +PT+   + ++LV Q     +   +  D EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VPID+ ++D+FDPF VPT+     EL                         R  +D   TS  KY+ +F++F++ +
Sbjct:   11 FQNREFSFTLKDDIYVRYQSFSTQNELEKEMQKMNPYKIDIGAVYSHRPSQHNTVKSGTFQALEKELVFDIDMTDYDDVRSCCSAADICSKCWTLMTIAIRILDRALREDFGFQHLLWVYSGRRGVHCWVCDEAARKLSVAARSAVAEYLSLVKGGEETVKKVVLSDP-----IHPFISQSLSVVEHYFLQYAIVGQELLSSKEGVDKVLNLLPEDV---RKDLLLHY--QSEKNPTKRWERLKTLVHQKRATSKKGGQYFDKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLRNLDKFDPFEVPTISLICEELDKPRPDEEEEDDTKDKENEIEAGERRKIRDFKRTSLAKYIKLFDQFLDSV 376          
BLAST of DNA primase vs. Ensembl Sea Lamprey
Match: prim1 (DNA primase subunit 1 [Source:ZFIN;Acc:ZDB-GENE-990603-6])

HSP 1 Score: 362.844 bits (930), Expect = 2.056e-123
Identity = 194/423 (45.86%), Postives = 253/423 (59.81%), Query Frame = 1
Query:   31 YNMSEISVHHDELIKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKK-KKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYEN----STKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL------------------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            Y+ SE+     +L+ ++Y+ +FP   + RWL+YG  E   F NREFSFTLKDDIYVRY SF+++ +  +E+ +  PYKIDIGAV++ +P  H     G F    KELVFDIDMTDYDDVR CC  ADIC  CWPLMT+AIKVIDRAL EDFGF+  LWVYSGRRGVHCWVCD  AR L   ARSAI  YLT VKGG    KKV L  +     +LHP I  ++++++ +FE+Y  T Q+ L  EK   ++L+++PD   E         L  D       +++W  +  +         +   + E+ ++F YPRLDVNVS G+NHLLKSPFS+HP TGR+CVPID+  VDEFDPF VPT+ Q   EL                              +  +D   TS    + +FERF++ L
Sbjct:    6 YDSSELP----DLLAVYYRRLFPYGPYYRWLNYGGVEKNYFVNREFSFTLKDDIYVRYQSFQNQADMEKEIQKCVPYKIDIGAVFSHKPNQHNSVKMGSFQALEKELVFDIDMTDYDDVRSCCSSADICPNCWPLMTIAIKVIDRALSEDFGFDHRLWVYSGRRGVHCWVCDIAARKLSQPARSAIAEYLTLVKGGEGMIKKVNLGENN----SLHPSISAAVQVVERYFEEYALTKQDILGTEKAWEKVLALLPDSIREE--------LRKDLLIQPNSVIRWEVIKQKFSKGGGNITARHYVSEVMLQFCYPRLDVNVSKGINHLLKSPFSIHPKTGRVCVPIDVARVDEFDPFTVPTISQICRELDRAVEPVTSPGQSKLDVERAKDGGTGELAAKRRRDYKRTSMAASVRVFERFVDKL 412          
BLAST of DNA primase vs. Ensembl Yeast
Match: PRI1 (Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair [Source:SGD;Acc:S000001447])

HSP 1 Score: 229.565 bits (584), Expect = 6.116e-72
Identity = 124/384 (32.29%), Postives = 203/384 (52.86%), Query Frame = 1
Query:   70 IKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNAL-NQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQL--EYENSTKVI------DEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRSTQDIHSTSFGKYLDIFERFINDL 1188
            ++ +YK+++P      WL++    +    NREF+   +   Y RY SF    +F  ++ + NP + +IGA+YN  P+              KELVFDIDM DYD  R CC  A +C KCW  +++A+K+ + ALREDFG++  +WV+SGRRG HCWV DK AR L    R  ++ Y+  ++  N  K++ L       R  HPH+  S+E +KP F   +   QN   + +  I  LL  + D+  +  D++K  WL++ +    +       +   L    +  + +I      +++ +  +YP+LDV V+    HLLK+PF +HP+TG +CVPID    + F P   P L    +E+    D+  T+   +++ F+ +++ L
Sbjct:   15 MEYYYKSLYPFKHIFNWLNHSPKPSRDMINREFAMAFRSGAYKRYNSFNSVQDFKAQIEKANPDRFEIGAIYNKPPRERDTLLKSELKALEKELVFDIDMDDYDAFRTCCSGAQVCSKCWKFISLAMKITNTALREDFGYKDFIWVFSGRRGAHCWVSDKRARALTDVQRRNVLDYVNVIRDRNTDKRLALK------RPYHPHLARSLEQLKPFFVSIMLEEQNPWEDDQHAIQTLLPALYDK--QLIDSLKKYWLDNPRRSSKEKWNDIDQIATSLFKGPKQDSHIIKLRECKEDLVLMTLYPKLDVEVTKQTIHLLKAPFCIHPATGNVCVPID----ESFAPEKAPKLIDLQTEMEKNNDVSLTALQPFINQFQAYVSSL 386          
BLAST of DNA primase vs. Ensembl Nematostella
Match: EDO37404 (DNA primase [Source:UniProtKB/TrEMBL;Acc:A7SFT3])

HSP 1 Score: 329.331 bits (843), Expect = 2.734e-110
Identity = 173/397 (43.58%), Postives = 244/397 (61.46%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGN--DEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGG-NKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL-------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            EL++ +YK +FP  L+ +WLSYG+     F NREFSFTLKDD+Y+RY SF  + E  +E+ ++NP+KIDIGAV++ +PK+HK      F    KELVFDIDMTDYD++R CC+ A+IC KCW  M +AIKV+D AL+EDFGF+  LWVYSGRRGVHCWVCD+ AR L    RSA+  YLT +KGG N+ KKV +          HP I  S+ ++K +F   +   Q  L+ ++  + LL+++       + +   +W         +  V   S   +++   S +VIDEI +++ YPRLDVNV+ G+NHLLKSPF +HP TGR+CVPI++ + D FDPF VPT+ Q ++++                   R  +    TS    L +F +F+  L
Sbjct:   21 ELLQQYYKWLFPYKLYHQWLSYGDVPKTYFPNREFSFTLKDDVYLRYQSFASQEEMEKEIQKRNPHKIDIGAVFSHKPKDHKMVKAASFQALEKELVFDIDMTDYDEIRTCCKGAEICLKCWKFMVIAIKVLDLALKEDFGFKHRLWVYSGRRGVHCWVCDETARKLSQSGRSAVAEYLTIIKGGENQAKKVNIREP------YHPFIKRSLSLVKKYFNDIVLDGQEILSSKETWDPLLALLNKSWSSCKKSSSERW--------EELTVMCTSFFQKMQ---SMEVIDEIMLQYTYPRLDVNVTKGLNHLLKSPFCIHPKTGRVCVPIEVATADLFDPFAVPTISQLLNQIDEYDKKHPNDNSDEDIAGKRKIRAFQKTSLMAGLSVFHKFLKLL 400          
BLAST of DNA primase vs. Ensembl Medaka
Match: prim1 (DNA primase subunit 1 [Source:NCBI gene;Acc:101156974])

HSP 1 Score: 360.147 bits (923), Expect = 1.090e-121
Identity = 184/402 (45.77%), Postives = 250/402 (62.19%), Query Frame = 1
Query:   64 ELIKIFYKTIFPSSLFCRWLSYGNDEA--FCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISEL-------------------------RSTQDIHSTSFGKYLDIFERFINDL 1188
            +L+ ++Y+ +FP S + RWL+YG  +   F NREFSFTLKDDIYVRY SF  +NE  +E+ + NPYKIDIGAVY+  P  H    +G F    KELVFDIDMTDYDDVR CC  ADIC KCW LMT+AI ++DRALR+DFGF+ +LWVYSGRRGVHCWVCD+ AR L    RSA+  YL+ VKGG +  K  +  D      +HP I  S+E+++ +F +Y    Q+ L +++ ++++L+++P++    R+ ++ K     Q +    ++K  +   Q   +       EI +++ YPRLDVNVS GVNHLLKSPFSVHP TGRI VPID+  VD FDPF VPT+ +   EL                         R  +D   TS  KY+  F+ F++ +
Sbjct:   15 DLLPLYYRRLFPFSQYYRWLNYGGVQKNYFQNREFSFTLKDDIYVRYQSFSTQNELEKEMQKMNPYKIDIGAVYSHRPNQHNTVKSGSFQALEKELVFDIDMTDYDDVRTCCSAADICSKCWTLMTIAIHILDRALRDDFGFQHLLWVYSGRRGVHCWVCDEAARKLSAAGRSAVAEYLSLVKGGEETVKKVVLTDP-----IHPFISASLEVVEKYFLEYAVQDQDILGRKECVDKVLALVPEDV---RNELQTKMKGVKQPEKLWKMIKNEAKNKQGTVKKGQHFEKEIMLQYCYPRLDVNVSKGVNHLLKSPFSVHPKTGRISVPIDLKEVDSFDPFVVPTISRICEELDRPRTEEEKSEESQDKENEKDAVETRKMRDYKRTSLAKYVKYFDSFLDGM 408          
BLAST of DNA primase vs. Planmine SMEST
Match: SMESG000070103.1 (SMESG000070103.1)

HSP 1 Score: 792.727 bits (2046), Expect = 0.000e+0
Identity = 383/384 (99.74%), Postives = 383/384 (99.74%), Query Frame = 1
Query:   37 MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGNDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRSTQDIHSTSFGKYLDIFERFINDL 1188
            MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGNDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIK HFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRSTQDIHSTSFGKYLDIFERFINDL
Sbjct:    1 MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGNDEAFCNREFSFTLKDDIYVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEWKELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFEKILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTLYNDKKWLRNLHPHIVESIEIIKSHFEKYLTTYQNALNQEKGINRLLSIIPDECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYMEFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVPTLEQTISELRSTQDIHSTSFGKYLDIFERFINDL 384          
The following BLAST results are available for this feature:
BLAST of DNA primase vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 5
Match NameE-valueIdentityDescription
PRIM12.989e-11946.67DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC... [more]
PRIM11.537e-11142.57DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC... [more]
PRIM13.736e-10148.48DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC... [more]
PRIM16.287e-5158.39DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC... [more]
PRIM11.188e-2637.01DNA primase subunit 1 [Source:HGNC Symbol;Acc:HGNC... [more]
back to top
BLAST of DNA primase vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 5
Match NameE-valueIdentityDescription
pri-12.778e-7438.20DNA primase small subunit [Source:UniProtKB/Swiss... [more]
T24C4.55.692e-5547.45DNA primase [Source:UniProtKB/TrEMBL;Acc:Q9TZ34][more]
T24C4.55.692e-5547.45DNA primase [Source:UniProtKB/TrEMBL;Acc:Q9TZ34][more]
pri-13.701e-1856.90DNA primase small subunit [Source:UniProtKB/Swiss... [more]
pri-13.701e-1856.90DNA primase small subunit [Source:UniProtKB/Swiss... [more]
back to top
BLAST of DNA primase vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 1
Match NameE-valueIdentityDescription
DNApol-alpha504.885e-10139.08gene:FBgn0011762 transcript:FBtr0076603[more]
back to top
BLAST of DNA primase vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prim12.989e-12145.10DNA primase subunit 1 [Source:ZFIN;Acc:ZDB-GENE-99... [more]
back to top
BLAST of DNA primase vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 5
Match NameE-valueIdentityDescription
rhagl3.583e-11445.36Rh associated glycoprotein like [Source:Xenbase;Ac... [more]
rhagl3.993e-11445.36Rh associated glycoprotein like [Source:Xenbase;Ac... [more]
rhagl2.712e-11245.15Rh associated glycoprotein like [Source:Xenbase;Ac... [more]
rhagl3.177e-11148.18Rh associated glycoprotein like [Source:Xenbase;Ac... [more]
rhagl4.949e-11147.62Rh associated glycoprotein like [Source:Xenbase;Ac... [more]
back to top
BLAST of DNA primase vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Prim11.262e-11745.93DNA primase, p49 subunit [Source:MGI Symbol;Acc:MG... [more]
Prim11.947e-11745.93DNA primase, p49 subunit [Source:MGI Symbol;Acc:MG... [more]
back to top
BLAST of DNA primase vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|P49642|PRI1_HUMAN1.435e-11846.67DNA primase small subunit OS=Homo sapiens OX=9606 ... [more]
sp|P20664|PRI1_MOUSE8.825e-11745.93DNA primase small subunit OS=Mus musculus OX=10090... [more]
sp|Q24317|PRI1_DROME4.897e-10039.08DNA primase small subunit OS=Drosophila melanogast... [more]
sp|O14215|PRI1_SCHPO8.469e-8642.54DNA primase small subunit OS=Schizosaccharomyces p... [more]
sp|Q25998|PRI1_PLAFK2.454e-8237.89DNA primase small subunit OS=Plasmodium falciparum... [more]
back to top
BLAST of DNA primase vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A210Q0073.369e-12348.21DNA primase OS=Mizuhopecten yessoensis OX=6573 GN=... [more]
A0A1S3HJ031.596e-12246.53DNA primase OS=Lingula unguis OX=7574 GN=LOC106155... [more]
B3RZA46.331e-12249.74DNA primase OS=Trichoplax adhaerens OX=10228 GN=TR... [more]
A0A3B4VJW67.561e-12246.13DNA primase OS=Seriola dumerili OX=41447 PE=3 SV=1[more]
A0A556U2J12.440e-12146.68DNA primase OS=Bagarius yarrelli OX=175774 GN=Baya... [more]
back to top
BLAST of DNA primase vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 2
Match NameE-valueIdentityDescription
prim11.703e-12446.67DNA primase subunit 1 [Source:NCBI gene;Acc:103027... [more]
prim11.933e-11747.34DNA primase subunit 1 [Source:NCBI gene;Acc:103027... [more]
back to top
BLAST of DNA primase vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prim12.056e-12345.86DNA primase subunit 1 [Source:ZFIN;Acc:ZDB-GENE-99... [more]
back to top
BLAST of DNA primase vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
PRI16.116e-7232.29Subunit of DNA primase; DNA primase is required fo... [more]
back to top
BLAST of DNA primase vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO374042.734e-11043.58DNA primase [Source:UniProtKB/TrEMBL;Acc:A7SFT3][more]
back to top
BLAST of DNA primase vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
prim11.090e-12145.77DNA primase subunit 1 [Source:NCBI gene;Acc:101156... [more]
back to top
BLAST of DNA primase vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000070103.10.000e+099.74SMESG000070103.1[more]
back to top
Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010424 ID=SMED30010424|Name=DNA primase|organism=Schmidtea mediterranea sexual|type=transcript|length=1202bp
CATTTTCTATGTGTTTTGTTTTAATATTATTATAATATGTCCGAAATAAG
TGTTCATCATGATGAACTAATAAAAATATTCTATAAAACGATTTTCCCAT
CATCATTATTTTGTAGATGGCTAAGTTATGGCAATGATGAAGCATTTTGC
AATCGTGAATTTAGTTTCACACTTAAAGATGATATTTATGTCCGATATTT
ATCCTTCAAAGATGAAAATGAATTTATTGAAGAACTTATTAGAAAGAATC
CATATAAAATCGATATTGGAGCCGTTTATAATATTGAACCAAAGAACCAC
AAGAAAGGTTCAACAGGTTGGTTTGTACCAGAATGGAAAGAATTAGTATT
CGATATTGATATGACAGATTATGATGACGTAAGATTCTGTTGTGAAAAGG
CAGATATTTGTAAAAAATGTTGGCCGTTAATGACTGTTGCAATAAAAGTG
ATTGATAGAGCACTGAGAGAAGATTTTGGTTTTGAAAAAATTCTGTGGGT
ATATTCCGGCCGAAGAGGAGTACATTGCTGGGTTTGCGATAAAGGTGCAC
GTGATCTAGTGTTTGATGCAAGAAGCGCAATAGTTGGATATCTTACCTAT
GTAAAAGGTGGTAATAAAAAGAAAAAGGTCACTCTTTATAACGACAAGAA
ATGGCTACGCAATTTGCATCCCCATATCGTTGAGAGCATTGAAATCATCA
AACCCCATTTTGAAAAATACTTGACAACCTATCAAAATGCTTTAAATCAA
GAAAAGGGAATCAATCGATTGTTGTCGATTATCCCGGATGAATGCTGTGA
ATACAGAGATGCGATGAAGCTCAAATGGCTAAATGATGATCAATCCGACC
CTACTCAATGCTTGGTGAAGTGGAGAAGCCTGGTTTGTCAATTGGAGTAT
GAAAATTCCACGAAAGTGATCGATGAGATTTATATGGAGTTTATGTATCC
TCGTCTCGATGTGAATGTTTCCACTGGAGTCAATCATTTGTTGAAGAGTC
CATTTTCTGTTCACCCGTCGACAGGGAGGATTTGTGTCCCGATCGATATT
GGATCTGTCGATGAGTTTGATCCGTTCAATGTTCCAACTCTCGAACAGAC
GATTTCTGAATTAAGATCCACTCAAGACATCCATTCGACGAGTTTCGGAA
AATATCTCGATATTTTTGAAAGATTTATTAATGATTTATAAATATAACTT
TT
back to top

protein sequence of SMED30010424-orf-1

>SMED30010424-orf-1 ID=SMED30010424-orf-1|Name=SMED30010424-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=385bp
MSEISVHHDELIKIFYKTIFPSSLFCRWLSYGNDEAFCNREFSFTLKDDI
YVRYLSFKDENEFIEELIRKNPYKIDIGAVYNIEPKNHKKGSTGWFVPEW
KELVFDIDMTDYDDVRFCCEKADICKKCWPLMTVAIKVIDRALREDFGFE
KILWVYSGRRGVHCWVCDKGARDLVFDARSAIVGYLTYVKGGNKKKKVTL
YNDKKWLRNLHPHIVESIEIIKPHFEKYLTTYQNALNQEKGINRLLSIIP
DECCEYRDAMKLKWLNDDQSDPTQCLVKWRSLVCQLEYENSTKVIDEIYM
EFMYPRLDVNVSTGVNHLLKSPFSVHPSTGRICVPIDIGSVDEFDPFNVP
TLEQTISELRSTQDIHSTSFGKYLDIFERFINDL*
back to top
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000014zeta neoblast
PLANA:0000240body wall musculature
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR014052DNA_primase_ssu_euk/arc
IPR002755DNA_primase_S
Vocabulary: biological process
TermDefinition
GO:0006269DNA replication, synthesis of RNA primer
GO:0006260DNA replication
Vocabulary: molecular function
TermDefinition
GO:0003896DNA primase activity
GO:0003899DNA-directed 5'-3' RNA polymerase activity
GO:0016740transferase activity
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3DG3DSA:3.90.920.30coord: 3..384
e-value: 1.3E-147
score: 493.9
NoneNo IPR availableSUPERFAMILYSSF56747Prim-pol domaincoord: 9..361
IPR002755DNA primase, small subunitPFAMPF01896DNA_primase_Scoord: 105..338
e-value: 1.0E-53
score: 182.0
IPR014052DNA primase, small subunit, eukaryotic/archaealTIGRFAMTIGR00335TIGR00335coord: 13..352
e-value: 5.0E-59
score: 198.3
IPR014052DNA primase, small subunit, eukaryotic/archaealPANTHERPTHR10536DNA PRIMASE SMALL SUBUNITcoord: 8..384
IPR014052DNA primase, small subunit, eukaryotic/archaealCDDcd04860AE_Prim_Scoord: 21..346
e-value: 5.58565E-89
score: 266.402