DNA topoisomerase 2
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Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010418 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 19
Homology
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1374.76 bits (3557), Expect = 0.000e+0 Identity = 709/1201 (59.03%), Postives = 898/1201 (74.77%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGK---PDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW K K E + +++ DSG PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ V +Q+ + G + K GK K +++ ET PS + RRI P
Sbjct: 51 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAW-KEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQL-EETMPSPYGRRIIP 1244
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1374.38 bits (3556), Expect = 0.000e+0 Identity = 709/1201 (59.03%), Postives = 898/1201 (74.77%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGK---PDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW K K E + +++ DSG PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ V +Q+ + G + K GK K +++ ET PS + RRI P
Sbjct: 46 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAW-KEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQL-EETMPSPYGRRIIP 1239
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]) HSP 1 Score: 1367.44 bits (3538), Expect = 0.000e+0 Identity = 686/1157 (59.29%), Postives = 873/1157 (75.45%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNAN-----KLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVV 3565
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ + +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV HK ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM + E+ ED+TC+TF PDL +F M LD+DIVAL RRAYDIA T+ + VFLNG ++ VK F+ YVD+Y K LD LKV++E RWEV +T S + F Q+SFVNSIAT+KGG HV+Y+ADQ+ K+++ KKK K QVK +MWIFV LIENP+FDSQTKENMTL K FGS C L+EKFI CGIVE + W FK Q L+KKCS K ++K +PKLDDAN AG +NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ +TP+ KW++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ LK+L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ +++++N++R+++G P+ M P Y+NF GTI E+ N+YV++GE+ L+ TT+EI+ELPVR WT +YKE V+E +G +KTP ITDY+EY+TD TV+F+V+M K AEAE G HK FKLQT LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A+++KL NQARFILEKI+GK+I EN LI L+ YDSDPVKAW + + E E ++ P F YL M + LT EKK+ L + R+EK +EL +K KSP +W +DL +E+ V
Sbjct: 30 VERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGEMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNSLKVIHEQVNHRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVTKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQPKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQVQLNKKCSAVKHNRIKGIPKLDDANDAGGRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEMAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQIDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSSLARLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREIVNNIRRLMDGEEPLPMLPSYKNFKGTIEELAPNQYVISGEVAILNSTTIEISELPVRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVVKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNEKEQELDTLKRKSPSDLWKEDLATFIEELEAV 1181
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2B (DNA topoisomerase II beta [Source:HGNC Symbol;Acc:HGNC:11990]) HSP 1 Score: 1354.35 bits (3504), Expect = 0.000e+0 Identity = 675/1107 (60.98%), Postives = 846/1107 (76.42%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGK---PDFGYLTQMQI 3421
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW K K E + +++ DSG PDF Y+ M +
Sbjct: 46 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAW-KEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSL 1146
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Match: TOP2A (DNA topoisomerase II alpha [Source:HGNC Symbol;Acc:HGNC:11989]) HSP 1 Score: 170.244 bits (430), Expect = 3.998e-48 Identity = 76/131 (58.02%), Postives = 108/131 (82.44%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVT 502
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ + +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV HK ++Y+P +IFGQLLTS+N++DD+K+VT
Sbjct: 18 VERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVT 147
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: top-2 (DNA topoisomerase 2 top-2 [Source:UniProtKB/Swiss-Prot;Acc:Q23670]) HSP 1 Score: 1302.73 bits (3370), Expect = 0.000e+0 Identity = 663/1220 (54.34%), Postives = 882/1220 (72.30%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKL-MWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW--------------MKFNANKLTXXXXXXXXXXXXXXXSGK---PDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDL-----EILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
IE +YQKK+QLEHILLRPD+Y+GS++ +K MWV++ E++++ ++ I+Y P LYKI+DEILVNA+DNK RD KM TI+I I+ + N + ++NNG+GIPVT HK +VY+P +IFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS++F LETSS++Y +FKQTW NM +P+I K+ EDFT +TF PDL +F M LD+DI L +RRAYD+A ++G+ VFLNGK+I +K F+DYV +YT + EPLK+ YE G RW+VA+ S + F QVSFVNSIATTKGG HV+Y+ADQ+ AK ++ K+K+ +K P Q+K +MW+FV CLIENP+FDSQTKE MTL QK FGS C L+EKF + GI + + W FK L+KKCSKTK +KLK +PKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFPLRGKLLNVRE +KQ+ +NAE+ +IKI+GLQY +KYE + KTLRYGKLM+M DQDQDGSHIKGL+INFIH WP+L++ NFVE+FITPIVKA K + +SFYS+PE EW+ NT + +++KYYKGLGTSTSKEAKEYF DM RH I+F+Y+G +D ++ +AFSK K +ERK+WL+ WM + K R++ G+ E YLY K T+++TF++F+N+EL+LFSN DNERSIPCL+DGFKPGQRKVLFAC KR +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGSNNINLL+P GQFGTRL GGKDSAS RYI TQLS +T+ +F D+ VL++L ++NQ IEPEWY PIIPMVL+NG GIGTGWS+ IPN+NP++++ N+KR+I G + PWY+NF G I+++ +++ GE+ LD T+EITELP++ WT YKE V+E +DK I DYKEY+TD TV+F+V+++P K E E H+ FKLQ + + M+LFD G +R Y + I + F+ R E Y++RK YLLG+L AQ+ +L NQARFIL KIN +++ EN K A ++D L+ K+D+DPVK W ++ + + L E++E + + + D+ YL M ++ L+ E+K L+KE +EK+ E+ I+ K+ + +W +DL E+ ++E +QD S RG K + E PSK +RIEP
Sbjct: 63 IEDIYQKKSQLEHILLRPDTYIGSVEHTEKTPMWVYNMEESKLEQRDISYVPGLYKIYDEILVNAADNKQRDPKMNTIKITINKEKNEISVYNNGKGIPVTQHKVEKVYVPELIFGTLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTKFTLETSSRDYKSAFKQTWIKNMTRDEEPKIVKSTDEDFTKITFSPDLAKFKMKELDDDICHLMARRAYDVAGSSKGVAVFLNGKRIPIKGFEDYVQMYTSQ-FNNEGEPLKIAYEQVGDRWQVALALS-EKGFQQVSFVNSIATTKGGRHVDYVADQMVAKFIDSIKRKLTKTSMNIK--PFQIKNHMWVFVNCLIENPTFDSQTKETMTLQQKQFGSTCVLSEKFSKAASSVGITDAVMSWVRFKQMDDLNKKCSKTKTSKLKGIPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPLRGKLLNVREGNMKQIADNAEVNAMIKILGLQYKKKYETEDDFKTLRYGKLMVMADQDQDGSHIKGLVINFIHHFWPSLIQRNFVEEFITPIVKATKGKEEVSFYSLPEYSEWRMNTDNWKSYKIKYYKGLGTSTSKEAKEYFLDMVRHRIRFKYNGADDDNAVDMAFSKKKIEERKDWLSKWMREKKDRKQQGLAEEYLYNKDTRFVTFKDFVNRELVLFSNLDNERSIPCLVDGFKPGQRKVLFACFKRADKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSNNINLLLPIGQFGTRLQGGKDSASARYIFTQLSPVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGIGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVAVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGCLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILAKINNEIVLENKKKAAIVDVLIKMKFDADPVKKWKEEQKLKELRESGEIELDEDDLAAVAVEEDEAISSAAKAVETKLSDYDYLVGMALIKLSEEEKNKLIKESEEKMAEVRVIEKKTWQDLWHEDLDNFVSELDKQEAREKADQDASIKNAAKKLAADAKTGRGPKK-NVCTEVLPSKDGQRIEP 1277
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 818.15 bits (2112), Expect = 0.000e+0 Identity = 449/1129 (39.77%), Postives = 688/1129 (60.94%), Query Frame = 2
Query: 122 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKR-NLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMING-GTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQL-------DGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMN---PAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQI 3421
Y+KK+ EH+LLRPD+Y+G + +ED+++W+ D E +M+ K++TY P L KIFDEILVNA+DNK RDS M + + +D + + +WNNG G+PV IH +Y+PT++FG L TS+N++D + + GGRNGYGAKLCNIFS EF++ET + F+Q W +NM C + E+ + E D+T V F PDL+RF + +L +D++ L RR +++AA + + V+LNG++ +V F+DYV ++ + +++ RW V V N F + VSFVN+I T KGG+HV+Y+ D + K K +KPA +K N+ IF+ CLIENPSF+SQTKE +T K+FGS EC +K + G++E + L N + + ++D+ KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W + + KW++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +RSIP L+DG KP QRK+L+ L + E+KV+QLAG+VA +YHHGE SL+ TII + Q F GS+N+ LL P GQFGTR GG D+AS RYI T L+ T+L+F D+ +L+ +++ +EP W PI+P++LING +GIGTGWS+KI N NP D+ID ++R I+ T ++ P+Y F G + V K++ +G+I + ++EI ELP+ +WT+ YKE + +I + P + ++ E +T+K V F + ++ +++ + + +FKL+T LT N +LFD G ++++ N EI FF+VR ++Y KR A+ ++NQ FI NG + ++ L +KL + DP+ K +S K ++GYL +M +
Sbjct: 47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRSIPSLIDGLKPSQRKILWTLLNNMDESTEIKVSQLAGAVAHRQSYHHGEESLVRTIIRMGQTFCGSSNLPLLQPIGQFGTRHEGGNDAASARYIFTALAPTTRLLFPQADDDLLQKNVEEGMVVEPTWLCPIVPLILINGTEGIGTGWSTKIANRNPIDIIDMIRRKIDSISTEYEIPPFYEEFRGKLEVVTPTKFISSGKIQLIRPERKNASTFSIEIVELPIGIWTSKYKEKLSKIV----ETLP--VLEFSERHTEKRVHFRITLDRKKSSRFLQKSNSDLLNYFKLRTSLT-ENRVLFDRNGELKEFGNISEIAAEFFEVRRDLYEKRLKIQKEECEAKLIYVENQLNFIEMVTNGTIEIRSMGRNQLEEKLQEMGFRVDPMATIAK---------------------NSKKANYGYLLEMPV 1119
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: cin-4 (DNA topoisomerase-like protein cin-4 [Source:UniProtKB/Swiss-Prot;Acc:G5ECQ8]) HSP 1 Score: 762.681 bits (1968), Expect = 0.000e+0 Identity = 359/654 (54.89%), Postives = 476/654 (72.78%), Query Frame = 2
Query: 1349 MPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAE-AAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW 3307
+PKL+DAN AGTKNS QCTLILTEGDSAK+LAVSGL+V+GRD YGVFP R KLLNV + V Q+ ++AE+ IIKI+GLQY++KYE + KTLRYGKL+IM + D DGS KGLL+NF H WP L K +FVE FITPI KA + + +SFYS+PE EW+ NT + + +KYY GLGT TSKEAK+ FSDM RH I+F+Y+G +D K++ +AFSK K + R ++L M+D +R++ G+ E LY K T+++T ++F N E++ N SIPCL+DG KPGQRKVLFAC KR +E+KVAQLAG+VAE+SAYHHGE SLM TI+NLAQD+VGS+NINLL+P GQFGTRL GGKDSAS R I QLS++T+ +F D+ VL++L ++NQ IEPEWY PIIPMVL+NG G GTGWS+ IPN+NP++++ N+KR+I G + PWY+NF G I+++ +++ GE++ LD T+EITELP++ WT YKE V+E +DK I DYKEY+TD TV+F+V+++P K E E H+ FKLQ + + M+LFD G +R Y + I + F+ R E Y++RK YLLG+L AQ+ +L NQARFIL IN K++ EN K ++D L+ K+D+DPVK W
Sbjct: 40 IPKLEDANDAGTKNSQQCTLILTEGDSAKTLAVSGLSVVGRDKYGVFPFRRKLLNVCDLNVNQIADSAEVNAIIKILGLQYTKKYETEDDFKTLRYGKLLIMANHDSDGSQFKGLLLNFFHRFWPALFKRDFVEDFITPIAKATEGKEEVSFYSLPEYSEWRMNTDNWKSYTIKYYNGLGTLTSKEAKKCFSDMVRHRIRFKYNGADDDKAVNMAFSKKKIEARTDYLMKLMQDKNQRKQQGLAEECLYNKETRFVTLKDFFNYEIVCSWNL---HSIPCLVDGLKPGQRKVLFACFKRANKREVKVAQLAGAVAEISAYHHGEQSLMGTIVNLAQDYVGSHNINLLLPIGQFGTRLQGGKDSASARSIFAQLSQVTRTLFPAHDDNVLRFLYEENQRIEPEWYCPIIPMVLVNGAQGTGTGWSTNIPNYNPRELVKNIKRLIAGEPQKALAPWYKNFRGKIIQIDPSRFACYGEVSVLDDNTIEITELPIKQWTQDYKEKVLEGLMESSDKKSPVIVDYKEYHTDTTVKFVVKLSPGKLRELERGQDLHQVFKLQAVINTTCMVLFDAAGWLRTYTSPEAITQEFYDSRQEKYVQRKEYLLGVLQAQSKRLTNQARFILATINNKIVLENKKKTAIVDVLIKMKFDADPVKKW 690
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 555.058 bits (1429), Expect = 4.457e-179 Identity = 295/703 (41.96%), Postives = 449/703 (63.87%), Query Frame = 2
Query: 122 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2179
Y+KK+ EH+LLRPD+Y+G + +ED+++W+ D E +M+ K++TY P L KIFDEILVNA+DNK RDS M + + +D + + +WNNG G+PV IH +Y+PT++FG L TS+N++D + + GGRNGYGAKLCNIFS EF++ET + F+Q W +NM C + E+ + E D+T V F PDL+RF + +L +D++ L RR +++AA + + V+LNG++ +V F+DYV ++ + +++ RW V V N F + VSFVN+I T KGG+HV+Y+ D + K K +KPA +K N+ IF+ CLIENPSF+SQTKE +T K+FGS EC +K + G++E + L N + + ++D+ KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W + + KW++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +R+
Sbjct: 47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Match: R05D3.12 (Putative DNA topoisomerase 2, mitochondrial [Source:UniProtKB/Swiss-Prot;Acc:P34534]) HSP 1 Score: 555.058 bits (1429), Expect = 4.457e-179 Identity = 295/703 (41.96%), Postives = 449/703 (63.87%), Query Frame = 2
Query: 122 YQKKTQLEHILLRPDSYVGSIK-KEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKE---DFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAAC-TQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQ--------VSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGS--ECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAG-TKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHN-FVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERS 2179
Y+KK+ EH+LLRPD+Y+G + +ED+++W+ D E +M+ K++TY P L KIFDEILVNA+DNK RDS M + + +D + + +WNNG G+PV IH +Y+PT++FG L TS+N++D + + GGRNGYGAKLCNIFS EF++ET + F+Q W +NM C + E+ + E D+T V F PDL+RF + +L +D++ L RR +++AA + + V+LNG++ +V F+DYV ++ + +++ RW V V N F + VSFVN+I T KGG+HV+Y+ D + K K +KPA +K N+ IF+ CLIENPSF+SQTKE +T K+FGS EC +K + G++E + L N + + ++D+ KL+DA +AG T + +CTLILTEGDSAK+LA++GL V+GR+ YGVFPL+GKLLNV + ++N EI+N+++I+GL++ + + ++LRYG+L+I+ DQD+DGSHIKGL++NFIH WP+L+ + F++ F TP++KA K ++V SF+S+ E +W + + KW++KYYKGLGTSTS EA+EYFSD++ H + F+Y+G D +I +AF + K+DERKEW+ RR E +E+ G I+++ F++ +L+ F D +R+
Sbjct: 47 YEKKSPTEHVLLRPDTYIGGVAMREDQIIWLRDSENRKMIAKEVTYPPGLLKIFDEILVNAADNKARDSSMNRLEVWLDRETARISVWNNGSGLPVEIHPTEGIYVPTLVFGNLFTSSNYDDSEIKTVGGRNGYGAKLCNIFSKEFIVETVDTRIKRRFRQKWYDNMKKCNEAEVVEILDETVKDYTKVEFVPDLERFQIDKLSDDVIDLIGRRVFEVAATLPRDVDVYLNGQKCDVDGFEDYVKMFN--------DSSSLLFLHPTPRWHVGVAKR-NNFFGESHVVLPKIVSFVNNINTEKGGSHVDYVMD--KIVNIIKPIVDSKLGDPTKSVKPAVIKNNLSIFINCLIENPSFESQTKETLTTKAKNFGSIFECDA-KKTAEWAEQSGLIEDIVEEVL--NMKKKKLPGKRVSVSSVRDIVKLEDAEWAGITGTAEKCTLILTEGDSAKALALAGLEVLGRETYGVFPLKGKLLNVSNLDDARASKNEEISNLLRILGLKFEDSNSI--TRESLRYGRLLILADQDEDGSHIKGLIVNFIHKFWPSLVHTDGFIQSFRTPLLKAKKGDKVRSFFSMNEYRKWA-DVEEGGKWKIKYYKGLGTSTSNEAREYFSDLDHHTVNFKYTGTTDDDAIRMAFDRDKSDERKEWI--------RRSE---NEITNEDDGKTEISYQEFVDGQLMQFGMVDLKRT 721
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0332320) HSP 1 Score: 1330.47 bits (3442), Expect = 0.000e+0 Identity = 673/1173 (57.37%), Postives = 868/1173 (74.00%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKF---------------------NANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEIN 3559
IE++YQKK+QLEHILLRPDSY+GS++ +LMWV+D+ +N MV+K+I++ P LYKIFDEILVNA+DNK RD M TI+IDID + NMV +WNNG+GIPVT+HKE ++Y+PTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS+ F +ET+++EY KSFKQTW NNM D +I D+T +TF PDL +F M RLDEDIVAL SRRAYD+AA ++G++VFLNG ++ V+ FKDY+DL+ K+ D + P+K+V+E +RWEVA S + F QVSFVNSIAT KGG HV+++ D L ++LE KKK K P QV+ ++W+FV CLIENP+FDSQTKENMTL QK FGS+CTL+EKFI+ ++K GIVE + W FK Q K K +K+K +PKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA KQL+ENAEI N+ KIIGLQY +KY + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+ F+E+FITPIVKA K N+ LSFYS+PE EW+ +T + + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G D +SIV+AFSK + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN +E+KVAQL+GSVAE+SAYHHGE SL TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H D+ +L Y +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING P MHPWY+NF G + V + +Y+ G I L G LEI+ELPV VWT +YKE+V+E +G +K I++Y+EY+TD TVRF++ P ++ AE GF++ FKL T L+ + M FD+ +R++ I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK K++ EN + + D+L+ Y DPVK W + + + ++ E + ++ + + F YL M + LT EKK LLK+RD KL EL ++ K+PE +WLDDL+ LE ++N
Sbjct: 10 IEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIK---PFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Match: Top2 (gene:FBgn0284220 transcript:FBtr0081287) HSP 1 Score: 1330.47 bits (3442), Expect = 0.000e+0 Identity = 673/1173 (57.37%), Postives = 868/1173 (74.00%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYA--EAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKF---------------------NANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEIN 3559
IE++YQKK+QLEHILLRPDSY+GS++ +LMWV+D+ +N MV+K+I++ P LYKIFDEILVNA+DNK RD M TI+IDID + NMV +WNNG+GIPVT+HKE ++Y+PTMIFG LLTS+N+NDD+K+VTGGRNGYGAKLCNIFS+ F +ET+++EY KSFKQTW NNM D +I D+T +TF PDL +F M RLDEDIVAL SRRAYD+AA ++G++VFLNG ++ V+ FKDY+DL+ K+ D + P+K+V+E +RWEVA S + F QVSFVNSIAT KGG HV+++ D L ++LE KKK K P QV+ ++W+FV CLIENP+FDSQTKENMTL QK FGS+CTL+EKFI+ ++K GIVE + W FK Q K K +K+K +PKL+DAN AG KNS++CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGKLLNVREA KQL+ENAEI N+ KIIGLQY +KY + LKTLRYGK+MIMTDQDQDGSHIKGLLINFIH+ WP LL+ F+E+FITPIVKA K N+ LSFYS+PE EW+ +T + + + +KYYKGLGTSTSKEAKEYF DM+RH I F+Y G D +SIV+AFSK + RK WLTN M++ KRR+ELG+ E YLY KGTK IT+ +FIN EL+LFSN DNERSIP L+DG KPGQRKV+F C KRN +E+KVAQL+GSVAE+SAYHHGE SL TI+NLAQ+FVG+NNINLL P GQFGTRL+GGKD AS RYI T +S LT+LI+H D+ +L Y +DD Q IEP WY PIIPMVL+NG +GIGTGWS+KI N+NP++++ NL++MING P MHPWY+NF G + V + +Y+ G I L G LEI+ELPV VWT +YKE+V+E +G +K I++Y+EY+TD TVRF++ P ++ AE GF++ FKL T L+ + M FD+ +R++ I+IL+ ++K+R E Y +R+++L+G L+AQA +L +QARFILEK K++ EN + + D+L+ Y DPVK W + + + ++ E + ++ + + F YL M + LT EKK LLK+RD KL EL ++ K+PE +WLDDL+ LE ++N
Sbjct: 10 IEQMYQKKSQLEHILLRPDSYIGSVEFTKELMWVYDNSQNRMVQKEISFVPGLYKIFDEILVNAADNKQRDKSMNTIKIDIDPERNMVSVWNNGQGIPVTMHKEQKMYVPTMIFGHLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSTSFTVETATREYKKSFKQTWGNNMGKASDVQIKDFNGTDYTRITFSPDLAKFKMDRLDEDIVALMSRRAYDVAASSKGVSVFLNGNKLGVRNFKDYIDLHIKNTDDDSGPPIKIVHEVANERWEVACCPS-DRGFQQVSFVNSIATYKGGRHVDHVVDNLIKQLLEVLKKKNKGGINIK---PFQVRNHLWVFVNCLIENPTFDSQTKENMTLQQKGFGSKCTLSEKFINNMSKSGIVESVLAWAKFKAQN-DIAKTGGRKSSKIKGIPKLEDANEAGGKNSIKCTLILTEGDSAKSLAVSGLGVIGRDLYGVFPLRGKLLNVREANFKQLSENAEINNLCKIIGLQYKKKYLTEDDLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHTNWPELLRLPFLEEFITPIVKATKKNEELSFYSLPEFEEWKNDTANHHTYNIKYYKGLGTSTSKEAKEYFQDMDRHRILFKYDGSVDDESIVMAFSKKHIESRKVWLTNHMDEVKRRKELGLPERYLYTKGTKSITYADFINLELVLFSNADNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLSGSVAEMSAYHHGEVSLQMTIVNLAQNFVGANNINLLEPRGQFGTRLSGGKDCASARYIFTIMSPLTRLIYHPLDDPLLDYQVDDGQKIEPLWYLPIIPMVLVNGAEGIGTGWSTKISNYNPREIMKNLRKMINGQEPSVMHPWYKNFLGRMEYVSDGRYIQTGNIQILSGNRLEISELPVGVWTQNYKENVLEPLSNGTEKVKGIISEYREYHTDTTVRFVISFAPGEFERIHAEEGGFYRVFKLTTTLSTNQMHAFDQNNCLRRFPTAIDILKEYYKLRREYYARRRDFLVGQLTAQADRLSDQARFILEKCEKKLVVENKQRKAMCDELLKRGYRPDPVKEWQRRIKMEDAEQADEEDEEEEEAAPSVSSKAKKEKEVDPEKAFKKLTDVKKFDYLLGMSMWMLTEEKKNELLKQRDTKLSELESLRKKTPEMLWLDDLDALESKLN 1177
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 1372.07 bits (3550), Expect = 0.000e+0 Identity = 703/1212 (58.00%), Postives = 894/1212 (73.76%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXX--XXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAA--------------ETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+E M ++I++ P LYKIFDEILVNA+DNK RD MTTI+I ID + N + +WNNG+GIPV HK+ ++Y+P +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS +F +ET+ KEY +SFKQTW+ NM + +I EDFTCVTF PDL +F+M +LD+DIVAL +RRAYD+A +G+ V LNGK++ V +F+ YVD Y K LD LKVV E+ +RWEV +T S + F Q+SFVNSIATTKGG H++Y+ DQ+ K++E KKK K P QVK ++W+FV LIENP+FDSQTKENMTL K FGS+C L+EKFI CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYG++MIMTDQDQDGSHIKGLLINF H WP+LLKH F+E+FITPIVKA KN Q +SFYSIPE EW+++T W +KYYKGLGTSTSKEAKEYFSDMERH I F+Y+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F D ++LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF GTI E+ +N+Y+V+GE++ LD T+EI+ELPVR WT +YKESV+E G DKTP I+DYKEY+TD TV+F+V M K A+AEAAG HK FKLQ+ LT ++M+LFD G +++Y + EIL+ FF++RL Y RK++L+G L A+++KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K L E D + P+F Y+ M + LT EK E LLK+RD K EL +++K+ E +W +DL + +E++ V Q+ E VN GKA+K+ ET PS F RR+EP
Sbjct: 50 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDNEKKVTGGRNGYGAKLCNIFSLKFTVETACKEYKRSFKQTWTENMGKSNEAKIKYFDGEDFTCVTFQPDLAKFNMEKLDKDIVALLTRRAYDVAGSCRGVKVILNGKKLPVNSFRSYVDFYVKDKLDETGVALKVVNETVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYIVDQIVGKLIEVVKKKNKAGVSVK---PFQVKNHLWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKILGLQYKKSYDDPESLKSLRYGRIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEVSFYSIPEFEEWKKHTESYKTWHIKYYKGLGTSTSKEAKEYFSDMERHRIMFKYNGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTSTRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCVKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSSLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGTIHELGQNQYLVSGEVSVLDKNTIEISELPVRTWTQAYKESVLEPMLQGTDKTPPLISDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQEILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKLSIENKSKRDLIRMLVQRGYESDPVAAWNKAQEKSLEEEDGGGNDSDSSVDSGSFSGPNFNYILNMPLWCLTKEKVEELLKQRDLKKAELNELQSKTSENLWREDLAVFIEELDRVEEQEK----ESVNL---------GKAVKLVKGKVGKPKVKKMQLEETLPSPFGRRVEP 1243
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1341.25 bits (3470), Expect = 0.000e+0 Identity = 681/1156 (58.91%), Postives = 873/1156 (75.52%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQD 3577
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD++ M + IT+ P LYKIFDEILVNA+DNK RD M I+++ID + N + +WNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS +F +ET+ +E + FKQTW +NM + +I +D+TC+TF PDL +F M LD+D VAL +RRAYDIA T+G+ V LNGK++ + F+ YVDLY K +D PL VV+E +RWEV +T S + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E KKK K P QVK +MW+FV CLIENPSFDSQTKENMTL QK+FGS C+L++KF+ CGIVE + W FK QA L+KKCS K TK+K +PKLDDAN AG KNS CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y PESLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+KA Q +SFYSIPE EW+ W++KYYKGLGTSTSKEAKEYFSDM RH I F+YSG D ++I LAFSK +ERKEWLT++M + ++RRE + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPC++DG KPGQRKV+F C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LKY DDN +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG P+ M P Y+ F GTI E+ +N+YV NGE+ +D TT+EITELPV+ WT +YKE+V+E+ +G +K PA ITDYKEY+TD TVRF+V+M + EAEAAG HK FKLQ LT ++M+LFD G ++KY + +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVKAW +A + E+ +++DE+ ED+ PD+ YL M + LT EKK+ L K+RD K+ EL +K K+P +W +DL +E+ V ++
Sbjct: 29 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDTG-MNCRDITFVPGLYKIFDEILVNAADNKQRDKTMNCIKVNIDPENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSLKFTVETACRESKRCFKQTWFDNMGRAGESKIKPFDGDDYTCITFQPDLAKFKMQALDKDTVALLTRRAYDIAGSTKGVRVLLNGKRLPINNFRTYVDLYVKDKVDETGSPLTVVHEMVNERWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKKNKAGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKNFGSACSLSDKFVKQANGCGIVESIMNWVKFKAQAQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSNSCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKILGLQYKKNYSDPESLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKQEISFYSIPEFTEWKEKQSSIKSWKIKYYKGLGTSTSKEAKEYFSDMTRHRIPFKYSGPADDEAITLAFSKKMIEERKEWLTSFMINRRQRREHNLPEEYLYGQETKSLSYHDFINKELVLFSNSDNERSIPCMVDGLKPGQRKVVFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWK--DAQEKEEEEDAEDDDEKEKEDTSGPDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKE 1177
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 414.075 bits (1063), Expect = 1.289e-125 Identity = 221/418 (52.87%), Postives = 301/418 (72.01%), Query Frame = 2
Query: 2324 SLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQD 3577
SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LKY DDN +EPEWY PIIP+VL NG +GIGTGW+S+IPN++ +++I+N+ RM+NG P+ M P Y+ F GTI E+ +N+YV NGE+ +D TT+EITELPV+ WT +YKE+V+E+ +G +K PA ITDYKEY+TD TVRF+V+M + EAEAAG HK FKLQ LT ++M+LFD G ++KY + +IL+ FF++R++ Y+ RK++L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVKAW + E AED++++E + SG PD+ YL M + LT EKK+ L K+RD K+ EL +K K+P +W +DL +E+ V ++
Sbjct: 2 SLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSTLARLLFPVVDDNLLKYNYDDNLRVEPEWYMPIIPLVLANGGEGIGTGWASRIPNYDVREIINNIHRMLNGEEPLPMLPSYKGFKGTIDELAKNQYVNNGEVAIIDSTTIEITELPVKTWTQTYKENVLEVMLNGTEKVPALITDYKEYHTDTTVRFVVKMTEERLREAEAAGLHKVFKLQNLLTCNSMVLFDHVGSLKKYESVQDILKEFFELRMKYYVLRKDWLVGMLGAESAKLSNQARFILEKIQGTLVIENKPKKELIRMLQQMGYDSDPVKAWKDAQEKE-EEEDAEDDDEKEKEDTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKMTELNTLKMKAPADLWKEDLAAFTEELERVEQKE 417
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 127.872 bits (320), Expect = 1.371e-33 Identity = 58/96 (60.42%), Postives = 77/96 (80.21%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRG 397
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+E M ++I++ P LYKIFDEILVNA+DNK RD MTTI+I ID + N + +WNNG+G
Sbjct: 45 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREISFVPGLYKIFDEILVNAADNKQRDKNMTTIKITIDPESNTISVWNNGKG 139
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1401.34 bits (3626), Expect = 0.000e+0 Identity = 713/1198 (59.52%), Postives = 897/1198 (74.87%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA--AETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+++ + + +T+ P LYKIFDEILVNA+DNK RD M+ I+I I+ + N V +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D D +I ED+TC+TF PDL +F M LD+DIVAL SRRAYD+A ++G+ VFLNGK++ + F+ YVDLY K+ LD PLKV++E RWE+ +T S + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E KKK K P QVK +MW+FV LIENP+FDSQTKENMTL K FGS+CTL+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ NT W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK++ DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELPVR WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEAAG HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W + + EE+EE ++ PDF YL M + L+ EKK+ L K+RD K EL +K KSP +W +DL +E++ V ++ E VVK GK G +K E PS RR+ P
Sbjct: 27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQ--TQEKEEAGELLEEEEETEVNTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVK--GKSGRPQVKKMQLQEIMPSPHGRRVVP 1215
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1399.42 bits (3621), Expect = 0.000e+0 Identity = 713/1198 (59.52%), Postives = 897/1198 (74.87%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA--AETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+++ + + +T+ P LYKIFDEILVNA+DNK RD M+ I+I I+ + N V +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D D +I ED+TC+TF PDL +F M LD+DIVAL SRRAYD+A ++G+ VFLNGK++ + F+ YVDLY K+ LD PLKV++E RWE+ +T S + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E KKK K P QVK +MW+FV LIENP+FDSQTKENMTL K FGS+CTL+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ NT W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK++ DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELPVR WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEAAG HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W + + EE+EE ++ PDF YL M + L+ EKK+ L K+RD K EL +K KSP +W +DL +E++ V ++ E VVK GK G +K E PS RR+ P
Sbjct: 27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQ--TQEKEEAGELLEEEEETEVNTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVK--GKSGRPQVKKMQLQEIMPSPHGRRVVP 1215
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1399.03 bits (3620), Expect = 0.000e+0 Identity = 713/1198 (59.52%), Postives = 897/1198 (74.87%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA--AETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+++ + + +T+ P LYKIFDEILVNA+DNK RD M+ I+I I+ + N V +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D D +I ED+TC+TF PDL +F M LD+DIVAL SRRAYD+A ++G+ VFLNGK++ + F+ YVDLY K+ LD PLKV++E RWE+ +T S + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E KKK K P QVK +MW+FV LIENP+FDSQTKENMTL K FGS+CTL+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ NT W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK++ DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELPVR WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEAAG HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W + + EE+EE ++ PDF YL M + L+ EKK+ L K+RD K EL +K KSP +W +DL +E++ V ++ E VVK GK G +K E PS RR+ P
Sbjct: 27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQ--TQEKEEAGELLEEEEETEVNTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVK--GKSGRPQVKKMQLQEIMPSPHGRRVVP 1215
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1397.1 bits (3615), Expect = 0.000e+0 Identity = 713/1198 (59.52%), Postives = 897/1198 (74.87%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA--AETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+++ + + +T+ P LYKIFDEILVNA+DNK RD M+ I+I I+ + N V +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D D +I ED+TC+TF PDL +F M LD+DIVAL SRRAYD+A ++G+ VFLNGK++ + F+ YVDLY K+ LD PLKV++E RWE+ +T S + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E KKK K P QVK +MW+FV LIENP+FDSQTKENMTL K FGS+CTL+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ NT W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK++ DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELPVR WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEAAG HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W + + EE+EE ++ PDF YL M + L+ EKK+ L K+RD K EL +K KSP +W +DL +E++ V ++ E VVK GK G +K E PS RR+ P
Sbjct: 27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQ--TQEKEEAGELLEEEEETEVNTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVK--GKSGRPQVKKMQLQEIMPSPHGRRVVP 1215
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Match: TOP2A (DNA topoisomerase II alpha [Source:NCBI gene;Acc:100038293]) HSP 1 Score: 1392.1 bits (3602), Expect = 0.000e+0 Identity = 713/1198 (59.52%), Postives = 897/1198 (74.87%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA--AETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+++ + + +T+ P LYKIFDEILVNA+DNK RD M+ I+I I+ + N V +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ +EY K FKQTW++NM+D D +I ED+TC+TF PDL +F M LD+DIVAL SRRAYD+A ++G+ VFLNGK++ + F+ YVDLY K+ LD PLKV++E RWE+ +T S + F Q+SFVNSIATTKGG HV+Y+ADQ+ AK++E KKK K P QVK +MW+FV LIENP+FDSQTKENMTL K FGS+CTL+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NSL CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPI+K K+ Q LSFYSIPE EW+ NT W++KYYKGLGTSTSKEAKEYFSDMERH I F+Y G D +I LAFS+ K D+RKEWLTN+M+D + RR G+ E YLYGK TKY+TF +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TIINLAQ+FVGSNN++LL P GQFGTRL GGKD+ASPRYI T LS L +L+F D++VLK++ DDNQ +EPEWY PIIP VL+NG +GIGTGWS KIPN++ ++++ N+ R+++G P+ M P Y+NF G I E+ N+Y++NGE++ LD TT+EI+ELPVR WT +YKE V+E +G +KTP ITDYKEY+TD TV+F+V++ K EAEAAG HK FKLQTPLT ++M+LFD G +RKY +IL FF +RL+ Y RK++L+G+L+A+AS+L NQARFILEKI GK++ EN LI LV YDSDPVK+W + + EE+EE ++ PDF YL M + L+ EKK+ L K+RD K EL +K KSP +W +DL +E++ V ++ E VVK GK G +K E PS RR+ P
Sbjct: 27 VERIYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEDEG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDPTMSCIKITINPESNTVSVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSNKFTVETACREYKKVFKQTWTDNMSDAADAKIKPFDGEDYTCITFQPDLSKFKMQILDKDIVALMSRRAYDVAGSSKGVKVFLNGKRLPISGFRSYVDLYLKNKLDETGNPLKVMHELVNDRWEICLTMS-EKGFQQISFVNSIATTKGGRHVDYVADQIIAKLIEVVKKKNKGGIAVK---PFQVKNHMWVFVNSLIENPTFDSQTKENMTLQSKSFGSKCTLSEKFIKGATNCGIVESILNWVKFKAQTQLNKKCSSVKHTKIKGIPKLDDANDAGSRNSLDCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKLLNVREASHKQIMENAEINNIIKIVGLQYKKNYDDNESLKTLRYGKVMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHRFLEEFITPIIKVTKSKQELSFYSIPEFEEWKNNTDSHKTWKIKYYKGLGTSTSKEAKEYFSDMERHRIPFKYEGPIDDAAITLAFSRKKVDDRKEWLTNFMQDRRERRLHGLPEEYLYGKSTKYLTFNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGETSLMMTIINLAQNFVGSNNVSLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAMDDSVLKFMYDDNQKVEPEWYIPIIPTVLVNGAEGIGTGWSCKIPNYDTREIVSNIGRLLDGLEPLPMLPSYKNFKGLIQELGPNQYILNGEVSVLDNTTIEISELPVRTWTQNYKEQVLEPMLNGTEKTPPLITDYKEYHTDTTVKFVVKLTEQKLMEAEAAGLHKVFKLQTPLTCNSMVLFDHVGCLRKYETATDILREFFVLRLKYYSLRKDWLVGMLTAEASRLSNQARFILEKIEGKIVIENKPKKELIQMLVQRGYDSDPVKSWKQ--TQEKEEAGELLEEEEETEVNTAGPDFNYLLGMPLWNLSKEKKDELCKQRDVKNAELQDLKKKSPSDLWKEDLSAFVEELDRVEAKEREEQSVTTGKVVK--GKSGRPQVKKMQLQEIMPSPHGRRVVP 1215
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2a (topoisomerase (DNA) II alpha [Source:MGI Symbol;Acc:MGI:98790]) HSP 1 Score: 1380.93 bits (3573), Expect = 0.000e+0 Identity = 692/1156 (59.86%), Postives = 882/1156 (76.30%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW----MKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVV 3565
IE++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ + +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV HK ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM D E+ + ED+TC+TF PDL +F M LD+DIVAL RRAYDIA T+ + VFLNG + VK F+ YVDLY K +D LKV++E RWEV +T S F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++ KKK K QVK +MWIFV LIENP+FDSQTKENMTL K FGS C L+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ +TP+ KW++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ L++L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ LD TT+EI+ELP+R WT +YKE V+E +G +KTP+ ITDY+EY+TD TV+F+++M K AEAE G HK FKLQ+ LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A++SKL NQARFILEKI+GK++ EN LI L+ YDSDPVKAW K + E + ++ ++G P F YL M + LT EKK+ L K+R+EK +EL +K KSP +W +DL + +E+ VV
Sbjct: 29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAG-PTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 1375.15 bits (3558), Expect = 0.000e+0 Identity = 708/1209 (58.56%), Postives = 897/1209 (74.19%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXS--GKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAA-----------ETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ +LMWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ +K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW + E ++++ D+ + + PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ V Q E +I+ + GKAIK ET PS + RRI P
Sbjct: 39 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQ---EREDILAGM-------SGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVP 1232
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 186.422 bits (472), Expect = 9.081e-53 Identity = 86/156 (55.13%), Postives = 117/156 (75.00%), Query Frame = 2
Query: 2585 INGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDE 3052
ING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M +E
Sbjct: 2 INGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMAAEEE 157
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Match: Top2b (topoisomerase (DNA) II beta [Source:MGI Symbol;Acc:MGI:98791]) HSP 1 Score: 55.4546 bits (132), Expect = 3.707e-7 Identity = 56/144 (38.89%), Postives = 79/144 (54.86%), Query Frame = 2
Query: 3194 RFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXX---SGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVK 3616
RFILEKI GK+ EN LI LV Y+SDPVKAW + E ++++ D+ + + SG PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ G+ TVVK
Sbjct: 1 RFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSG-PDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELD----------GDPDTTVVK 133
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|O42131|TOP2B_CHICK (DNA topoisomerase 2-beta OS=Gallus gallus OX=9031 GN=TOP2B PE=2 SV=1) HSP 1 Score: 1382.47 bits (3577), Expect = 0.000e+0 Identity = 710/1202 (59.07%), Postives = 896/1202 (74.54%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXX--XXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKP--GKRGGKAIKIAA--ETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ +LMWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I+I ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM +P+I +D+TC+TF PDL +F M LD+DIV+L +RRAYD+A +G+ V LNGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ K++E KKK K P QVK ++W+FV CLIENPSFDSQTKENMTL K FGS+C L+EKF + CGI+E + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE PESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+NLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+YVV+GEI +D T+EITELPVR WT YKE V+E +G +KTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW + E + +++ A + PDF Y+ M + +LT EK E L+K RD K EL +K KS +W +DL +E+ V Q E +++ +V KP GK G +K ET PS F RRI P
Sbjct: 56 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKISIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEPKIKHFEGDDYTCITFQPDLSKFKMENLDKDIVSLMTRRAYDLAGSCKGVKVMLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHEVVNERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFVNCLIENPSFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIIESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSLDCTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYEDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHMENHKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNLLQPIGQFGTRLHGGKDAASPRYIFTMLSPLARLLFPSVDDNLLKFLYDDNQRVEPEWYIPIIPMVLVNGAEGIGTGWACKLPNYDTREIVNNVRRMLDGLDPHPMLPNYKNFRGTIQELGQNQYVVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTEKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTSLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLHYYSLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKRDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDPQNANDDASSASGSTSGPDFNYILNMSLWSLTKEKVEELIKHRDSKERELNDLKRKSASDLWKEDLAAFVEELEKVEAQ---EREDVLAGMVGKPIKGKVGKPKMKKLQLEETMPSPFGRRIVP 1249
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|P41515|TOP2A_CRIGR (DNA topoisomerase 2-alpha OS=Cricetulus griseus OX=10029 GN=TOP2A PE=1 SV=1) HSP 1 Score: 1381.31 bits (3574), Expect = 0.000e+0 Identity = 696/1154 (60.31%), Postives = 885/1154 (76.69%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNAN--KLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVV 3565
IE++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ + +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV HK ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS+ F +ET+SKEY K FKQTW +NM D E+ ED+TC+TF PDL +F M LD+DIVAL RRAYDIA T+ + VFLNG ++ VK F+ YVD+Y K LD LKVV+E RWEV +T S + F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++ KKK K QVK +MWIFV LIENP+FDSQTKENMTL K FGS C L+EKFI CGIVE + W FK Q L+KKCS K ++K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q L+FYS+PE EW+ +TP+ KW++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLT++MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS LT+L+F D+ LK+L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ L+ TT+EI+ELP+R WT +YKE V+E +G +KTP ITDY+EY+TD TV+F+++M K AEAE G HK FKLQT LT ++M+LFD G ++KY+ ++IL+ FF++RL+ Y RK +LLG+L A+++KL NQARFILEKI+GK+I EN LI L+ YDSDPVKAW + + D E+ +++ +SG P F YL M + LT EKK+ L K+R+EK +EL +KNKSP +W +DL + +E+ VV
Sbjct: 29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNLISIWNNGKGIPVVEHKVEKMYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTRFTVETASKEYKKMFKQTWMDNMGRAGDMELKPFNGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNKLPVKGFRSYVDMYLKDKLDETGNALKVVHEQVNPRWEVCLTMS-EKGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHNRIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQELAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTHFMEDRRQRKLLGLPEDYLYGQTTTYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLTRLLFPPKDDHTLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDIREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILNSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPPLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQTSLTCNSMVLFDHVGCLKKYDTVLDILKDFFELRLKYYGLRKEWLLGMLGAESAKLNNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNENSDSVAESG-PTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKNKSPSDLWKEDLAVFIEELEVV 1176
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q01320|TOP2A_MOUSE (DNA topoisomerase 2-alpha OS=Mus musculus OX=10090 GN=Top2a PE=1 SV=2) HSP 1 Score: 1380.93 bits (3573), Expect = 0.000e+0 Identity = 692/1156 (59.86%), Postives = 882/1156 (76.30%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW----MKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVV 3565
IE++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ + +++T+ P LYKIFDEILVNA+DNK RD KM+ IR+ ID + N++ IWNNG+GIPV HK ++Y+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+S+EY K FKQTW +NM D E+ + ED+TC+TF PDL +F M LD+DIVAL RRAYDIA T+ + VFLNG + VK F+ YVDLY K +D LKV++E RWEV +T S F Q+SFVNSIAT+KGG HV+Y+ADQ+ +K+++ KKK K QVK +MWIFV LIENP+FDSQTKENMTL K FGS C L+EKFI CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG++NS +CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + YE +SLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q ++FYS+PE EW+ +TP+ KW++KYYKGLGTSTSKEAKEYF+DM+RH I+F+YSG ED +I LAFSK + D+RKEWLTN+MED ++R+ LG+ E YLYG+ T Y+T+ +FIN+ELILFSN+DNERSIP ++DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+S+YHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ L++L DDNQ +EPEWY PIIPMVLING +GIGTGWS KIPNF+ ++V++N++R+++G P+ M P Y+NF GTI E+ N+YV+NGE+ LD TT+EI+ELP+R WT +YKE V+E +G +KTP+ ITDY+EY+TD TV+F+++M K AEAE G HK FKLQ+ LT ++M+LFD G ++KY+ ++IL FF++RL+ Y RK +LLG+L A++SKL NQARFILEKI+GK++ EN LI L+ YDSDPVKAW K + E + ++ ++G P F YL M + LT EKK+ L K+R+EK +EL +K KSP +W +DL + +E+ VV
Sbjct: 29 IERIYQKKTQLEHILLRPDTYIGSVELVTQQMWVYDEDVG-INYREVTFVPGLYKIFDEILVNAADNKQRDPKMSCIRVTIDPENNVISIWNNGKGIPVVEHKVEKIYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETASREYKKMFKQTWMDNMGRAGDMELKPFSGEDYTCITFQPDLSKFKMQSLDKDIVALMVRRAYDIAGSTKDVKVFLNGNSLPVKGFRSYVDLYLKDKVDETGNSLKVIHEQVNPRWEVCLTMS-ERGFQQISFVNSIATSKGGRHVDYVADQIVSKLVDVVKKKNKGGVAVKAH---QVKNHMWIFVNALIENPTFDSQTKENMTLQAKSFGSTCQLSEKFIKAAIGCGIVESILNWVKFKAQIQLNKKCSAVKHTKIKGIPKLDDANDAGSRNSTECTLILTEGDSAKTLAVSGLGVVGRDKYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKNYEDEDSLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHRFLEEFITPIVKVSKNKQEIAFYSLPEFEEWKSSTPNHKKWKVKYYKGLGTSTSKEAKEYFADMKRHRIQFKYSGPEDDAAISLAFSKKQVDDRKEWLTNFMEDRRQRKLLGLPEDYLYGQSTSYLTYNDFINKELILFSNSDNERSIPSMVDGLKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSSYHHGEMSLMMTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDSASPRYIFTMLSPLARLLFPPKDDHTLRFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWSCKIPNFDVREVVNNIRRLLDGEEPLPMLPSYKNFKGTIEELASNQYVINGEVAILDSTTIEISELPIRTWTQTYKEQVLEPMLNGTEKTPSLITDYREYHTDTTVKFVIKMTEEKLAEAERVGLHKVFKLQSSLTCNSMVLFDHVGCLKKYDTVLDILRDFFELRLKYYGLRKEWLLGMLGAESSKLNNQARFILEKIDGKIVIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDTETSTSDSAAEAG-PTFNYLLDMPLWYLTKEKKDELCKQRNEKEQELNTLKQKSPSDLWKEDLAVFIEELEVV 1178
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q64511|TOP2B_MOUSE (DNA topoisomerase 2-beta OS=Mus musculus OX=10090 GN=Top2b PE=1 SV=2) HSP 1 Score: 1375.15 bits (3558), Expect = 0.000e+0 Identity = 708/1209 (58.56%), Postives = 897/1209 (74.19%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXS--GKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAA-----------ETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ +LMWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ +K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM+ G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW + E ++++ D+ + + PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ V Q E +I+ + GKAIK ET PS + RRI P
Sbjct: 39 VERVYQKKTQLEHILLRPDTYIGSVEPLTQLMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCKGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVSKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSSLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLEGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEEEDSQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDTKGREVNDLKRKSPSDLWKEDLAAFVEELDKVEAQ---EREDILAGM-------SGKAIKGKVGKPKVKKLQLEETMPSPYGRRIVP 1232
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Match: sp|Q02880|TOP2B_HUMAN (DNA topoisomerase 2-beta OS=Homo sapiens OX=9606 GN=TOP2B PE=1 SV=3) HSP 1 Score: 1374.76 bits (3557), Expect = 0.000e+0 Identity = 709/1201 (59.03%), Postives = 898/1201 (74.77%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGK---PDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWV+D++ M +++T+ P LYKIFDEILVNA+DNK RD MT I++ ID + N++ IWNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM + +I ED+TC+TF PDL +F M +LD+DIVAL +RRAYD+A +G+ V NGK++ V F+ YVDLY K LD LKV++E +RW+V +T S + F Q+SFVNSIATTKGG HV+Y+ DQ+ K++E KKK K P QVK ++W+F+ CLIENP+FDSQTKENMTL K FGS+C L+EKF + CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+SL+CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ ESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LLKH F+E+FITPIVKA KN Q LSFYSIPE EW+++ + W++KYYKGLGTST+KEAKEYF+DMERH I FRY+G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E +LYG TK++T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNINLL P GQFGTRL GGKD+ASPRYI T LS L +L+F D+ +LK+L DDNQ +EPEWY PIIPMVLING +GIGTGW+ K+PN++ +++++N++RM++G P M P Y+NF GTI E+ +N+Y V+GEI +D T+EITELPVR WT YKE V+E +G DKTPA I+DYKEY+TD TV+F+V+M K A+AEAAG HK FKLQT LT ++M+LFD G ++KY +IL+ FF +RL Y RK +L+G+L A+++KL NQARFILEKI GK+ EN LI LV Y+SDPVKAW K K E + +++ DSG PDF Y+ M + +LT EK E L+K+RD K E+ +K KSP +W +DL +E++ V +Q+ + G + K GK K +++ ET PS + RRI P
Sbjct: 51 VERVYQKKTQLEHILLRPDTYIGSVEPLTQFMWVYDEDVG-MNCREVTFVPGLYKIFDEILVNAADNKQRDKNMTCIKVSIDPESNIISIWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWMNNMMKTSEAKIKHFDGEDYTCITFQPDLSKFKMEKLDKDIVALMTRRAYDLAGSCRGVKVMFNGKKLPVNGFRSYVDLYVKDKLDETGVALKVIHELANERWDVCLTLS-EKGFQQISFVNSIATTKGGRHVDYVVDQVVGKLIEVVKKKNKAGVSVK---PFQVKNHIWVFINCLIENPTFDSQTKENMTLQPKSFGSKCQLSEKFFKAASNCGIVESILNWVKFKAQTQLNKKCSSVKYSKIKGIPKLDDANDAGGKHSLECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREASHKQIMENAEINNIIKIVGLQYKKSYDDAESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLKHGFLEEFITPIVKASKNKQELSFYSIPEFDEWKKHIENQKAWKIKYYKGLGTSTAKEAKEYFADMERHRILFRYAGPEDDAAITLAFSKKKIDDRKEWLTNFMEDRRQRRLHGLPEQFLYGTATKHLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINLLQPIGQFGTRLHGGKDAASPRYIFTMLSTLARLLFPAVDDNLLKFLYDDNQRVEPEWYIPIIPMVLINGAEGIGTGWACKLPNYDAREIVNNVRRMLDGLDPHPMLPNYKNFKGTIQELGQNQYAVSGEIFVVDRNTVEITELPVRTWTQVYKEQVLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAAGLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLGAESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAW-KEAQEKAAEEDETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSPSDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQL-EETMPSPYGRRIIP 1244
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A2R2MQU0 (DNA topoisomerase 2 OS=Lingula unguis OX=7574 GN=LOC106174550 PE=3 SV=1) HSP 1 Score: 1426.38 bits (3691), Expect = 0.000e+0 Identity = 729/1202 (60.65%), Postives = 919/1202 (76.46%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFN-----ANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIA-AETAPSKFSRRIEP 3697
+E++YQKK+QLEHILLRPD+Y+GS++K + MWV ++E + M+++ +T+ P LYKIFDEILVNA+DNK RD KMTTI+IDIDS+ N V+IWNNG+GIPV HK +V +PT+IFG LLTS+N+ND++++VTGGRNGYGAKLCNIFS++FV+ETSSK+ G +FKQTW++NM + +I+ A ED+TC+TF PDL +F M LD+DIV LFSRRAYDIAA T+G+ VFLNGK++ + +F+ YVDL+ K D N +PL VV+E RWEVAVT S + F Q SFVNSIATTKGG H++Y DQ+ K+ + KKK AVK P Q+K +MW+FV CLI NP+FDSQTKENMTL K FGS+C LN+KFI V+KCGIVE + W FK Q L+KKC +K +KLK +PKLDDAN AGTKNS CTLILTEGDSAK+LAV+GL+VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI N+IKI+GLQY KY+ PE+LKTLRYGK+MIMTDQDQDGSHIKGLL+NFIH WP+LLKHNFVE+FITPIVK K + +FYS+PE EW+ TP+ + +R KYYKGLGTSTSKEAKEYFSDMERH I F+YSG D SI LAFSK K +ERK+WL+N+MED KRR ELG+ +VYLYGK T++IT+ +FIN+ELILFSN DNERSIP L+DGFKPGQRKV+F CLKRNL KE+KVAQLAGSV+E+SAYHHGE SLM TIINLAQ+FVGSNN+NLL P GQFGTRL GGKD+ASPRYI T LS L + +F+ DE + KY DDN +EP++Y PI+PMVL+NG +GIGTGWS+KIPN++ ++++ NLKR+I+G P M P ++NF GTI E + + +GE++ LD T+EITELP+RVWT +YKESV+E G DKTPA I+DYKEY+TD TV+F+V+M K A+AE G HKFFKLQT ++ SNM+LFD+ G ++KY+ ++L F+ VR+E YIKRK+YL G+LSA+A KL+NQARFI EKI+G++ EN LI LV YDSDP+KAW + A++ +E D+E EA +G PDF Y+ + + +L+ EKKE LLK+RD K EEL ++ K+P +W DDLE +E+ V ++ + VN V K K K KI ET PS RR+EP
Sbjct: 65 VERIYQKKSQLEHILLRPDTYIGSVEKVTQPMWVINEEADRMIQRDLTFVPGLYKIFDEILVNAADNKQRDPKMTTIKIDIDSENNRVKIWNNGKGIPVIEHKTEKVMVPTLIFGHLLTSSNYNDEEQKVTGGRNGYGAKLCNIFSNKFVVETSSKDEGHAFKQTWTHNMGKATEAKITPCASEDYTCITFEPDLAKFKMDSLDKDIVDLFSRRAYDIAASTKGVKVFLNGKKLPINSFRSYVDLFLKDKFDDNGQPLNVVHEVINPRWEVAVTLS-DSGFKQASFVNSIATTKGGRHIDYFVDQVVNKLADVVKKKQGKGGMAVK--PFQIKNHMWVFVNCLIVNPTFDSQTKENMTLQAKSFGSKCELNDKFIQQVSKCGIVESILSWVKFKTQTQLNKKCHASKHSKLKGIPKLDDANDAGTKNSAHCTLILTEGDSAKTLAVAGLSVIGRDQYGVFPLRGKVLNVREATHKQIMENAEINNLIKIVGLQYKNKYDSPETLKTLRYGKIMIMTDQDQDGSHIKGLLVNFIHHNWPSLLKHNFVEEFITPIVKVTKGKEEKAFYSLPEFEEWKEATPNWHTYRCKYYKGLGTSTSKEAKEYFSDMERHRIPFKYSGPSDDASITLAFSKKKIEERKQWLSNFMEDRKRRAELGLPDVYLYGKDTRHITYNDFINKELILFSNMDNERSIPSLIDGFKPGQRKVMFTCLKRNLQKEIKVAQLAGSVSEMSAYHHGEMSLMGTIINLAQNFVGSNNLNLLQPIGQFGTRLHGGKDAASPRYIFTALSPLARAVFNAHDEPLYKYCYDDNLKVEPQFYVPILPMVLVNGAEGIGTGWSTKIPNYDIREIVSNLKRLIDGEEPRPMIPAFKNFIGTI-EDHSGETICHGEVSILDDETIEITELPIRVWTQNYKESVLEPMLQGTDKTPAVISDYKEYHTDVTVKFVVKMTAEKLAQAEQVGLHKFFKLQTSIS-SNMVLFDKNGCLKKYDRAEDVLREFYDVRMEYYIKRKDYLEGLLSAEALKLENQARFICEKIDGEISIENKPKKELIQLLVRRGYDSDPLKAWREAQDKLAEADRGGVETESDDEQSEASSTAGGPDFNYILNLPLWSLSKEKKEELLKQRDAKQEELRALRRKTPSCLWKDDLEKFLEELEKVEQKEREDAAAGVNPGVVKTAKGRAKPKKIHIEETKPSPMGRRVEP 1261
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A0L8FFS9 (DNA topoisomerase 2 (Fragment) OS=Octopus bimaculoides OX=37653 GN=OCBIM_22023035mg PE=3 SV=1) HSP 1 Score: 1419.83 bits (3674), Expect = 0.000e+0 Identity = 721/1197 (60.23%), Postives = 916/1197 (76.52%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQ-DTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKK+QLEHILLRPD+Y+GS++ + MWV D++ + MV+++I+Y P LYKIFDEILVNA+DNK RD KM TI+IDIDS+ N ++IWNNG+GIPV HK ++Y+PTMIFG LLTS+N++D +K+VTGGRNGYGAKLCNIFS++FV+ETS +EY ++FKQTW+ NM + +P I+++ +DFT +TF PDL +F + LD+D VALF RRA+DIAA T+G+ V LNGK++ VK FKDYVDLY K +D +PLKV+YES RWE+AVT S + F Q SFVNSIATTKGG H+++++D L KI+E KKK K P Q+K +MW+FV CLIENP+FDSQTKENMTL K FGS+C +EKFI+ V+KCG+VE + W FK QA L KKC +K +KLK +PKLDDAN+AGTKNS CTLILTEGDSAKSLAV+GL ++GRD YGVFPL+GK+LNVREA KQ+ ENAEI N+IKI+GLQ+ +KYE E L+TLRYGK+MIMTDQD DGSHIKGL+INFIH WPNLLKHN VE+FITPIVK KN +FYSIPE EWQR T + + W++KYYKGLGTST+KEAKEYFSDMERH I F+Y G ED SI LAFSK K DERKEWLTNWME+ +RR E+G+ E+YLYGKG I+F FIN+ELILFS TDN RSIP L+DG KPGQRKV+F C KRN KE+KVAQLAGSVAELSAYHHGEASLMSTIINLAQ+FVGSNNINLL+P GQFGTRL GGKD+ASPRYI T LS + K IFH DD +LK L DDNQ IEPE+Y PIIPM+L+NG DGIGTGWS+KIPN++P+++I N++RM++G P+ M PW++NF GTI E+ ENKY+V+GEI+ LD TT+EITELP+R WT +YKE V+E HG++K P+ I DYKEY+TD +VRF+V+M+ K A AEA+G HKFFKLQT + ++M+LFD+ G IRKY+ +I+ FF +RL+ Y +RK+Y+ G+ +++ K N ARFI+EKI+G V ENV LI LV YDSDP+KAW + + ++ D+E E++ PDF Y+ + + LT EKKE +++R+ K +EL R++ K PE ++ +DL++L E++ Q E +I+ T G+ K I + ET PS RRI+P
Sbjct: 40 VERIYQKKSQLEHILLRPDTYIGSVQLHTQQMWVIDEDLSTMVQREISYVPGLYKIFDEILVNAADNKQRDPKMNTIKIDIDSENNRIKIWNNGKGIPVVEHKTEKMYVPTMIFGHLLTSSNYDDSEKKVTGGRNGYGAKLCNIFSTKFVVETSCQEYKRAFKQTWTGNMLNASEPVITESRADDFTAITFCPDLPKFQLESLDKDHVALFCRRAFDIAASTRGVKVMLNGKRVPVKNFKDYVDLYIKDKVDDAGQPLKVIYESVSDRWEIAVTIS-DSGFQQASFVNSIATTKGGRHIDHVSDPLVNKIIEVVKKKNKGGLNIK---PFQIKSHMWLFVNCLIENPTFDSQTKENMTLQPKSFGSKCQPSEKFITAVSKCGVVESVMSWMKFKEQAQLQKKCHSSKHSKLKGIPKLDDANWAGTKNSSHCTLILTEGDSAKSLAVAGLGIVGRDCYGVFPLKGKVLNVREATHKQIMENAEINNLIKILGLQFKEKYEDIEKLRTLRYGKIMIMTDQDNDGSHIKGLIINFIHHFWPNLLKHNVVEEFITPIVKVTKNKVEKAFYSIPEFEEWQRGTQNWHTWKIKYYKGLGTSTAKEAKEYFSDMERHRIPFKYEGPEDDASINLAFSKKKIDERKEWLTNWMEEKRRRLEMGLPELYLYGKGVNQISFNEFINRELILFSFTDNNRSIPSLVDGLKPGQRKVIFTCFKRNDKKEVKVAQLAGSVAELSAYHHGEASLMSTIINLAQNFVGSNNINLLLPIGQFGTRLHGGKDAASPRYIFTLLSSVGKRIFHSDDLPLLKNLYDDNQKIEPEYYVPIIPMILVNGTDGIGTGWSTKIPNYDPREIITNIRRMLSGNDPLNMVPWFKNFKGTIEELSENKYLVSGEISVLDETTVEITELPIRTWTQNYKEEVLEPMLHGSEKNPSVIIDYKEYHTDTSVRFVVKMSGEKLANAEASGLHKFFKLQTTFSTNSMVLFDQNGCIRKYDKVEQIMREFFDLRLDYYKRRKDYMEGMFKSESLKFDNIARFIMEKIDGLVKIENVSKKDLIQLLVRRGYDSDPIKAWRESQSKNKE---KDESSDDEEKEETTGPDFNYILGLPLWCLTKEKKEEYIRQREAKADELERLRRKRPEDIYRNDLDVLLTELDQFEEQVREDEKVDIIKTQKTSKGRVKQKKITL-EETLPSPVGRRIQP 1228
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A0X3PUD6 (DNA topoisomerase 2 OS=Schistocephalus solidus OX=70667 GN=TR96187 PE=3 SV=1) HSP 1 Score: 1418.68 bits (3671), Expect = 0.000e+0 Identity = 728/1238 (58.80%), Postives = 905/1238 (73.10%), Query Frame = 2
Query: 89 NERVPED-----------IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANE--EPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXX---------------XXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGG-------KAIKIAAETAPSKFSRRIEP 3697
N VPED +E++YQKKTQLEHIL+RPD+Y+GS+++ LMW++D EK M++K+ITY P LYKI+DEILVNA+DNK+RD M I+IDI ++ N++RIWNNG GIPV H+ ++++P++IFG LLTS+N++D +K+VTGGRNG+GAKLCNIFS +FV+ETSSKE+ K FKQTW +NM +P I+ +A EDFTC++FYPDL RF MT L+ D VALF RRAYDIAA T+G+ V LNG ++ +K FKDYVDLY K D E E + VVYE RWEVAV S N F QVSFVNSIATTKGG H++Y++DQ+A K+++ KKK VK Q++ +MW+FV CLIENPSFDSQTKE MTL K FGS C L+EKFI+ V++ GIVE + W FK Q +DK+C K+K KLK +PKLDDAN AGTKNS CTLILTEGDSAK+LAV+GL+V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI N+IKI+GLQY KYE PES+ TLRYGK+MIMTDQDQDGSHIKGLLI+FIH WP LL HNF+E+FITPIVK FK Q L+FYSIPE EWQ+ T + + WR+KYYKGLGTSTSKEAK+YFSDM RH I+FRYSG ED SI LAF+K K+D+RK WLT WM + KRR ELG+ E YLYG GT IT+ +F+++ELILFSN DNERSIP L+DG KPGQRKVL+ CLKRNLVKE+KVAQL+GSVAE+S+YHHGEASLM TII LAQDFVGSNN+NLLMP+GQFGTRL+GGKD+ASPRYI T LS T+ IF D+A+L YL +DNQ IEP WY P+IPMVLING DGIGTGW +KIPN++ +VI NL+RMI+G P+ M P +R+F G I E+ +N+YVV GEI LD T+EITELPVRVWT SYKE V+E+ +G++K PA I DYKEY+TD TVRF+V M P K EAEA G HKFFKL T +T ++M+LFD G +++Y + EIL+ FF++RLE Y KRK+YL G+LSA+A +L+NQARF+LEKI GK+ EN LI L Y+ DPV AW K +KL + A S D+ Y+ M + +LT E+K+ LL +RD+K EL + +KS ++W DDL+ LE + + ++ KK K+ KA K +T P +RI P
Sbjct: 8 NGDVPEDGIVDPAKKRLSVERIYQKKTQLEHILIRPDTYIGSVERATTLMWLYDKEKQAMIQKEITYTPGLYKIYDEILVNAADNKVRDPSMNCIKIDIKAEDNLIRIWNNGAGIPVVHHRVEKMFVPSLIFGHLLTSSNYDDTEKKVTGGRNGFGAKLCNIFSRKFVVETSSKEHKKCFKQTWIDNMTKTSEPRITASAGEDFTCISFYPDLARFGMTELEADTVALFERRAYDIAASTRGVKVILNGVRVPIKNFKDYVDLYLK---DKGEDGEAVPVVYECVNPRWEVAVAAS-NLGFQQVSFVNSIATTKGGKHIDYISDQVANKLVDVVKKKSGKSGVTVKSF--QIRSHMWVFVNCLIENPSFDSQTKEFMTLQSKSFGSTCVLSEKFINQVSRSGIVENVLSWVRFKAQEKMDKQCHKSKHVKLKGIPKLDDANDAGTKNSAACTLILTEGDSAKTLAVAGLSVVGRDRYGVFPLRGKLLNVREASSKQIMENAEINNLIKILGLQYKNKYETPESVATLRYGKIMIMTDQDQDGSHIKGLLISFIHHNWPKLLHHNFLEEFITPIVKVFKGKQELAFYSIPEFEEWQKTTSNWHTWRVKYYKGLGTSTSKEAKDYFSDMTRHRIRFRYSGPEDDASITLAFNKNKSDDRKSWLTTWMNERKRRVELGLPEDYLYGSGTHAITYSDFVHKELILFSNMDNERSIPSLVDGLKPGQRKVLYTCLKRNLVKEIKVAQLSGSVAEMSSYHHGEASLMGTIIGLAQDFVGSNNLNLLMPHGQFGTRLSGGKDAASPRYIFTSLSPFTRKIFSERDDAILHYLFEDNQRIEPYWYIPVIPMVLINGADGIGTGWGTKIPNYDVMEVISNLRRMIDGADPLPMLPSFRDFRGVIQELGDNRYVVFGEIAILDEQTVEITELPVRVWTQSYKEGVLEVMLNGSEKVPASILDYKEYHTDATVRFVVRMTPEKLREAEAIGLHKFFKLSTAMTTNSMVLFDHMGCVKRYASVSEILKEFFELRLEWYNKRKSYLEGMLSAEARRLENQARFVLEKIQGKISIENRAKRDLIRLLKEHNYEPDPVHAW-KECIDKLAAIEDARAASRAAGEGEEEDSEEQGGDASTSSAAADYNYILGMPLWSLTKERKDDLLAQRDKKQAELTELLSKSARRLWRDDLDELEAVVKKYEEDRQKDMQSLIEAAEKKMSKQTASGRNVPSKASKSVKQTLPDPLGKRIVP 1238
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: G4VPA9 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 GN=Smp_065840 PE=3 SV=1) HSP 1 Score: 1414.05 bits (3659), Expect = 0.000e+0 Identity = 723/1211 (59.70%), Postives = 913/1211 (75.39%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTX------XXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKK--------PGKRGG-KAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPDSY+GS K+ + MWV D+ + MV + IT++P LYKIFDEILVNA+DNK+RD M+ I+IDI+ NMVRIWN+G+GIPV HK Y+PT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +F++ETSSKEY KSF+Q W +NM DP+I EDFTC+TFYPDL RF M L++D V+LF+RRAYD+AA GI VFLNGK++ +K F DYV+L+ K D + + +KVVYE+ +RW+VAV S N F QVSFVNSIATTKGGTH+NY++DQ+ K+++ KKK +K P Q+K +MW+FV CLIENP+FDSQTKENMTL K FGS CTL+EKFI +K GIVE + W FK +DK+C K+K +KLK +PKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H WPNLLKHN VE+FITPIVK FK Q SFYS+PE EWQ++TP+ + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED SI LAF K+K +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L YL DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N ++I+NL RM++G P +M P +R FTGTI ++ N+YV GE+ LD TLEITELP+RVWT +YKESV+E +G++K PA ITDYKEY+TD TVRF+++M+P K EAE+ G HKFFKLQT ++ +M+ FD G ++ Y N I I+ F+++RL Y KRK YL G+LSA+A KL+NQARF+LEKI ++ EN LI L A YDSDPVKAW K + +K + ++ + ++G+PD+ Y+ M + +LT E+K+ LL +RD K +EL+ +K+KSP +W +DL+ LE+E V + ++ + KK G+ K+ K+A ET P RRI P
Sbjct: 32 VERIYQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKKSGKSGVQIK--PFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAW-KESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVP 1237
BLAST of DNA topoisomerase 2 vs. TrEMBL
Match: A0A3Q0KH18 (DNA topoisomerase 2 OS=Schistosoma mansoni OX=6183 PE=3 SV=1) HSP 1 Score: 1413.67 bits (3658), Expect = 0.000e+0 Identity = 723/1211 (59.70%), Postives = 913/1211 (75.39%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTX------XXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKK--------PGKRGG-KAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPDSY+GS K+ + MWV D+ + MV + IT++P LYKIFDEILVNA+DNK+RD M+ I+IDI+ NMVRIWN+G+GIPV HK Y+PT+IFG LLTS+N++D +++VTGGRNGYGAKLCNIFS +F++ETSSKEY KSF+Q W +NM DP+I EDFTC+TFYPDL RF M L++D V+LF+RRAYD+AA GI VFLNGK++ +K F DYV+L+ K D + + +KVVYE+ +RW+VAV S N F QVSFVNSIATTKGGTH+NY++DQ+ K+++ KKK +K P Q+K +MW+FV CLIENP+FDSQTKENMTL K FGS CTL+EKFI +K GIVE + W FK +DK+C K+K +KLK +PKLDDAN AGTKNS QCTLILTEGDSAK+LAV+GL V+GRD YGVFPLRGKLLNVREA KQ+ ENAEI +IKI+GLQY KYE ++LK LRYGK+MIMTDQDQDGSHIKGL+INF+H WPNLLKHN VE+FITPIVK FK Q SFYS+PE EWQ++TP+ + WR+KYYKGLGTSTSKEAKEYFSDM RH I+FRYSG ED SI LAF K+K +RK WLTN+ ++ KRRRELG+ E YLYGK T+ IT+ +F+++EL+LFSN DNERSIP ++DG KPGQRKVLF CLKRNL++E+KVAQLAGSVAELSAYHHGE SLMSTII LAQ+FVGSNN+NLL P GQFGTRL+GGKD+ASPRYI T L+ LT+LIFH +D+ +L YL DDNQ IEPEWYAPIIPMVL+NG DGIGTG+++ I N+N ++I+NL RM++G P +M P +R FTGTI ++ N+YV GE+ LD TLEITELP+RVWT +YKESV+E +G++K PA ITDYKEY+TD TVRF+++M+P K EAE+ G HKFFKLQT ++ +M+ FD G ++ Y N I I+ F+++RL Y KRK YL G+LSA+A KL+NQARF+LEKI ++ EN LI L A YDSDPVKAW K + +K + ++ + ++G+PD+ Y+ M + +LT E+K+ LL +RD K +EL+ +K+KSP +W +DL+ LE+E V + ++ + KK G+ K+ K+A ET P RRI P
Sbjct: 32 VERIYQKKTQLEHILLRPDSYIGSTLKDTQQMWVLDESQQHMVNRDITFSPGLYKIFDEILVNAADNKVRDPTMSCIKIDINPAENMVRIWNDGKGIPVVHHKVENTYVPTLIFGHLLTSSNYDDTERKVTGGRNGYGAKLCNIFSKKFIVETSSKEYKKSFRQVWVDNMTKTSDPKILADKGEDFTCITFYPDLSRFGMNELEQDTVSLFTRRAYDMAATCNGIKVFLNGKRLPIKNFVDYVNLFIKPNKD-DADSVKVVYEAVNQRWQVAVAVS-NTGFQQVSFVNSIATTKGGTHINYVSDQIVNKLMDIVKKKSGKSGVQIK--PFQIKNHMWLFVNCLIENPTFDSQTKENMTLQIKSFGSTCTLSEKFIGQASKSGIVENVLSWIRFKAMEKMDKQCHKSKHSKLKGIPKLDDANNAGTKNSSQCTLILTEGDSAKTLAVAGLGVVGRDNYGVFPLRGKLLNVREASNKQIMENAEINALIKILGLQYKLKYESADTLKDLRYGKIMIMTDQDQDGSHIKGLIINFVHCNWPNLLKHNVVEEFITPIVKVFKGKQEHSFYSLPEFEEWQKSTPNWHTWRVKYYKGLGTSTSKEAKEYFSDMNRHRIRFRYSGIEDDGSIQLAFDKSKIADRKNWLTNFTQERKRRRELGLPEPYLYGKDTRAITYHDFVHKELVLFSNLDNERSIPSVVDGLKPGQRKVLFTCLKRNLIREIKVAQLAGSVAELSAYHHGEQSLMSTIIGLAQNFVGSNNLNLLQPIGQFGTRLSGGKDAASPRYIFTALNSLTRLIFHLEDDPLLNYLYDDNQRIEPEWYAPIIPMVLVNGADGIGTGYATHILNYNVIEIINNLYRMLDGEEPHRMLPNFRGFTGTIEDLGSNRYVCYGEVAVLDDDTLEITELPIRVWTQNYKESVLEPMLNGSEKVPACITDYKEYHTDVTVRFVIKMSPEKLREAESTGLHKFFKLQTVMSTGSMVCFDPLGCLKCYPNEIIIIREFYELRLTWYEKRKVYLEGVLSAEARKLENQARFVLEKIQSIMVIENKPKKELIRMLKEANYDSDPVKAW-KESIDKAAALQEQEEARTDEAVSQTEAVEAGQPDYNYILNMPLWSLTKERKDDLLAQRDAKQKELLILKSKSPSDLWREDLKKLEEEYKKVEAELAADEQAAIEAADKKLKIASTTTAGRVAATKSRKLAMETRPDPMGRRIVP 1237
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2b (DNA topoisomerase II beta [Source:ZFIN;Acc:ZDB-GENE-041008-136]) HSP 1 Score: 1379 bits (3568), Expect = 0.000e+0 Identity = 708/1200 (59.00%), Postives = 895/1200 (74.58%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXX--XXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQ--DTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD+E M ++ITY P LYKIFDEILVNA+DNK RD M++I+I ID + N V IWNNG+GIPV HK+ ++Y+P +IFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM DP+I +DFTCVTF PDL +F M +LD+DIVAL +RRAYD+A +G+ VFLNGK++ V F+ YVDLY K LD LKVV ES +RWEV +T S + F Q+SFVNSIATTKGG H++Y+ DQ+ +K++E KKK K P QVK ++W+FV LIENP+FDSQTKENMTL K FGS+C L+EKFI CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESLK+LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+E+FITPIVKA KN Q + FYSIPE EW++ T + W +KYYKGLGTSTSKEAKEYF+DMERH I F+Y G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG T+++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF C+KRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNN+N+L P GQFGTR+ GGKD+ASPRYI T LS L KL+F D +LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM++ P+ M P Y+NF G I E+ +N+YVV+GEI+ LD T+EITELP+R WT +YKESV+E G +KTPA I DYKEY+TD TV+F+V M K A+AEAAG HK FKLQ+ LT ++M+LFD G +++Y + +IL+ FF++RL Y RK++L+G L A+++KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW + L E + + S P+F Y+ M + LT EK + LLK+RD K EL ++ K E +W +DL + +E++ V Q +++ G+ V V K GK K I++ ET PS F RR+EP
Sbjct: 50 VERVYQKKTQLEHILLRPDTYIGSVEPVTQQMWVFDEEIG-MNLREITYVPGLYKIFDEILVNAADNKQRDKNMSSIKITIDPESNTVSIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYKHSFKQTWQNNMTKTSDPKIKYFDGDDFTCVTFQPDLSKFKMEKLDKDIVALLTRRAYDVAGSCKGVKVFLNGKKLPVNGFRSYVDLYVKDKLDETGVALKVVNESVNERWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVSKLIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCALSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEEFITPIVKASKNKQEIPFYSIPEFDEWKKQTENYKTWHIKYYKGLGTSTSKEAKEYFADMERHRIMFKYGGTEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEQYLYGTATRHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCMKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNVNILQPLGQFGTRINGGKDAASPRYIFTMLSPLAKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLDHQDPLPMLPSYKNFKGVIHELGQNQYVVSGEISVLDKNTIEITELPIRTWTQAYKESVLEPMLQGTEKTPALINDYKEYHTDTTVKFVVRMTEEKLAQAEAAGLHKVFKLQSSLTCNSMVLFDHMGCMKRYESVQDILKEFFELRLHYYKLRKDWLVGSLGAESAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQRGYESDPVAAWNRAQEKALEEEDRDGNESDSSIDSGSSSGPNFNYILNMPLWCLTKEKVDELLKQRDLKKAELNELQRKCSEDLWREDLAVFVEELDRVEEQERESASQGKGVKLVKGKVGKPKVKKIQL-EETLPSPFGRRVEP 1243
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1323.15 bits (3423), Expect = 0.000e+0 Identity = 682/1196 (57.02%), Postives = 875/1196 (73.16%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD++ + + IT+ P LYKIFDEILVNA+DNK RD+ M I+I+IDS+ N + +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ E SFKQTW +NM+ + +I + +D+TC+TF PDL +F M LD D VAL +RRAYDIA ++G+ V LNG +++V F+ YVDLY K +D PL VV+E RWEV +T S + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E KK+ K P QVK +MW+FV CLIENPSFDSQTKENMTL QK FGS C L+EKFI CGIV+ + W FK Q L+KKCS K TK+K +PKLDDAN AG K+S CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+K NQ SFYSIPE EW+ + W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG D ++I LAFS+ K +ERK+WLT++M + ++RRE + E YLYGK T +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L P GQFGTRL GGKDSASPRYI T LS L++L+F D+ +LKY DDN +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG P+ M P Y+ F GTI E+ N Y+++GE++ +D TT+EI+ELPV+ WT SYKE+V+E+ +G+DK PA ITDY+EY+TD TVRF+V+M + EAEAAG HK FKLQ+PLT ++M+LFD G ++KY +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVK W K K A +E++++ + SG PD+ YL M + LT EKK+ L K+RD KL EL ++ K+P +W DL +E+ V + ++ T VK G R K +K+ ET P+ RR+ P
Sbjct: 29 VERIYQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVG-LNCRDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVW-KQAQEKEVEIDAGEEDEQKEEQTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKV--TQVKGKGGR-TKVVKMKDETLPTPQGRRVIP 1200
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1322.76 bits (3422), Expect = 0.000e+0 Identity = 682/1196 (57.02%), Postives = 875/1196 (73.16%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD++ + + IT+ P LYKIFDEILVNA+DNK RD+ M I+I+IDS+ N + +WNNG+GIPV HK +V++P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ET+ E SFKQTW +NM+ + +I + +D+TC+TF PDL +F M LD D VAL +RRAYDIA ++G+ V LNG +++V F+ YVDLY K +D PL VV+E RWEV +T S + F QVSFVNSIATTKGG H +Y+ADQ+ +K++E KK+ K P QVK +MW+FV CLIENPSFDSQTKENMTL QK FGS C L+EKFI CGIV+ + W FK Q L+KKCS K TK+K +PKLDDAN AG K+S CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y P+SLK+LRYGKLMIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+K NQ SFYSIPE EW+ + W++KYYKGLGTSTSKEAKEYFSDM RH I FRYSG D ++I LAFS+ K +ERK+WLT++M + ++RRE + E YLYGK T +T+ +F+N+EL+LFSN+DNERSIPCL+DG KPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ L P GQFGTRL GGKDSASPRYI T LS L++L+F D+ +LKY DDN +EPEWY PIIP+VL+NG DGIGTGW+ K+PNF+ +++I+N+ RM+NG P+ M P Y+ F GTI E+ N Y+++GE++ +D TT+EI+ELPV+ WT SYKE+V+E+ +G+DK PA ITDY+EY+TD TVRF+V+M + EAEAAG HK FKLQ+PLT ++M+LFD G ++KY +IL+ FF++RL+ Y+ RK +L+G+L A+++KL NQARFILEKI G ++ EN LI L YDSDPVK W K K A +E++++ + SG PD+ YL M + LT EKK+ L K+RD KL EL ++ K+P +W DL +E+ V + ++ T VK G R K +K+ ET P+ RR+ P
Sbjct: 29 VERIYQKKTQLEHILLRPDTYIGSVEPLSQQMWVFDEDVG-LNCRDITFVPGLYKIFDEILVNAADNKQRDNSMNCIKINIDSENNTISVWNNGKGIPVVEHKVEKVFVPALIFGQLLTSSNYDDDQKKVTGGRNGYGAKLCNIFSTKFTVETACAESKYSFKQTWFDNMSRAGEAKIKPFSGDDYTCITFRPDLTKFKMQSLDRDTVALMTRRAYDIAGASKGVRVLLNGTKLSVNGFRSYVDLYLKDKVDETGTPLTVVHEVVNSRWEVCLTLS-EKGFQQVSFVNSIATTKGGRHTDYVADQIVSKLIEVVKKRNKGGVAVK---PFQVKNHMWLFVNCLIENPSFDSQTKENMTLQQKSFGSTCLLSEKFIKQANTCGIVDSIMNWVKFKAQNQLNKKCSAVKHTKIKGVPKLDDANDAGGKHSAGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNIIKIMGLQYRKNYSDPDSLKSLRYGKLMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKVSSKNQEFSFYSIPEFTEWKEKQNNIKSWKIKYYKGLGTSTSKEAKEYFSDMARHRIPFRYSGPADDEAITLAFSRKKIEERKDWLTSFMINRRQRREHNLPEEYLYGKETSSLTYHDFVNKELVLFSNSDNERSIPCLVDGLKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEISLMMTIVGL--------------PLGQFGTRLHGGKDSASPRYIFTMLSSLSRLLFPSVDDNLLKYNYDDNVKVEPEWYIPIIPLVLVNGGDGIGTGWACKLPNFDVREIINNIHRMLNGDEPLPMLPSYKGFKGTIEELTSNHYLISGEVSIIDSTTIEISELPVKTWTQSYKENVLEVMLNGSDKVPALITDYREYHTDSTVRFVVKMTEERLIEAEAAGLHKVFKLQSPLTCTSMVLFDHVGSLKKYEFVQDILKDFFELRLKYYVLRKAWLVGMLGAESAKLSNQARFILEKIEGTLVIENKPKKELIRMLQEMGYDSDPVKVW-KQAQEKEVEIDAGEEDEQKEEQTSG-PDYNYLLSMPMWYLTKEKKDELCKQRDAKLTELNMLEKKTPSDLWQADLAAFTEELERVEQAEKESQAKV--TQVKGKGGR-TKVVKMKDETLPTPQGRRVIP 1200
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: top2a (DNA topoisomerase II alpha [Source:ZFIN;Acc:ZDB-GENE-030131-2453]) HSP 1 Score: 1026.16 bits (2652), Expect = 0.000e+0 Identity = 516/880 (58.64%), Postives = 662/880 (75.23%), Query Frame = 2
Query: 1103 VKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYK---GLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWM----------KFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKR--GGKAIKIAAETAPSKFSRRIEP 3697
VK +MWIFV CL+ENP+FDSQTKENMTL K FGS C+L+EKFI CGIVE + W FK Q+ L++ CS K +KL+ +PKLDDAN AG KNS +CTLILTEGDSAK+LAVSGL V+GRD+YG+FPLRGK+LNVREA KQ+ ENAEI N+IKI+GLQY +KY+ PESLKTLRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+H F+E+FITPIVK KN Q L FYS+PE EW+ T + W++KYYK GLGTSTSKEAKEYF+DM+RH I+FRYSG ED +I LAFS+ K ++RKEWLTNWM D ++RRE G+ E +LYGK T Y+T+ +FIN+ELILFSN+DNERSIP L+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI++LAQ+FVGSNNIN+L P GQFGTRL GGKD+ASPRYI T +S + +L+F D+ +LK+L DDNQ +EPEWY PIIP+VL+NG DGIGTGW+S+IPNFNP+++I+N++R+++G P+ M PWY+NF G+I E+ N+++ +GEI+ LD T+EI+ELPVR WT +YKESV+E G DKT ITDYKEY+TD TVRF+++++ K EAEAAG HK FKLQ PLT ++M+LFD G ++KY + +IL F++VRL+ Y RK +L GIL A+++KL NQARFILEKI G V+ EN LI L A YDSDP++AW + N+ + + + E + SG PD+ Y+ M + LT E+K+ LL++RD K++EL +K KSP +W DDL + +E++ V ++ + + + K G++ G K++ + AE PS RRI P
Sbjct: 1 VKNHMWIFVNCLVENPTFDSQTKENMTLQHKKFGSTCSLSEKFIKTAMGCGIVESILNWVKFKAQSQLNQNCSAKKISKLRGIPKLDDANDAGGKNSQKCTLILTEGDSAKTLAVSGLGVVGRDHYGIFPLRGKMLNVREASYKQIMENAEINNLIKIVGLQYKKKYDDPESLKTLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLQHRFLEEFITPIVKVSKNKQELPFYSMPEFDEWKNETSNYKTWKIKYYKDMPGLGTSTSKEAKEYFADMKRHRIEFRYSGPEDDNAITLAFSRKKIEDRKEWLTNWMVDRRQRREQGLPESFLYGKQTAYLTYNDFINKELILFSNSDNERSIPSLVDGFKPGQRKVLFTCFKRNDKREVKVAQLAGSVAEMSAYHHGEQSLMMTIVSLAQNFVGSNNINVLQPIGQFGTRLNGGKDAASPRYIFTMISTVARLLFPPVDDNLLKFLWDDNQRVEPEWYCPIIPLVLVNGADGIGTGWASRIPNFNPREIINNIRRLLDGEEPLPMLPWYKNFKGSIDELGPNQFMFSGEISILDSETIEISELPVRTWTQTYKESVLEPMLSGTDKTTPLITDYKEYHTDTTVRFIIKISREKLLEAEAAGLHKVFKLQAPLTCNSMVLFDHLGCLKKYESVADILREFYEVRLKYYSLRKEWLNGILGAESAKLSNQARFILEKIEGTVVIENKPKKELIRMLEARGYDSDPIRAWKEAQVKSNFEEEANSQEDSDDGEESGSATSSTPSSG-PDYNYILNMALWCLTKERKDELLRQRDLKIQELEDLKCKSPCDLWKDDLAVFLEELDRVEAKERKD--DTADMARGKGGQKKAGSKSLAMKAEVVPSPHGRRIVP 877
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Match: ENSPMAT00000007762.1 (pep scaffold:Pmarinus_7.0:GL492656:1017:4077:-1 gene:ENSPMAG00000007010.1 transcript:ENSPMAT00000007762.1 gene_biotype:protein_coding transcript_biotype:protein_coding) HSP 1 Score: 374.015 bits (959), Expect = 5.564e-118 Identity = 182/326 (55.83%), Postives = 242/326 (74.23%), Query Frame = 2
Query: 53 FKMQSSLL---DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLAD 1021
F +Q +LL D++ ++R+ +E++YQKKTQLEHILLRPD+Y+GS++ + MWVFD E M + +TY P L+KIFDEILVNA+DNK RD MT I++ ID + N + +WNNG+GIPV HK +VY+P +IFGQLLTS+N++DD+K+VTGGRNGYGAKLCNIFS++F +ETS E K FKQ W NNM+ D I ++ EDFTC+TF PDL +F+MT LD+DI+A+ +RRAYDIA +G+ VFLNG+++ + F+ YVD Y K +D + PL VV+E RWEV +T S + F QVSFVNSIATTKGG HV+Y+ D
Sbjct: 8 FYLQKNLLNVKDDAKSKRL--SVERIYQKKTQLEHILLRPDTYIGSVEPVTQNMWVFD-EGIGMNCRDVTYVPGLFKIFDEILVNAADNKQRDPNMTCIKVTIDPENNKISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTQFTVETSCSESKKMFKQKWMNNMSKECDSVIKPSSGEDFTCITFQPDLTKFNMTILDKDIMAIMTRRAYDIAGICKGVKVFLNGQKLPLTGFRKYVDFYLKDRVDESGMPLHVVHEVVSNRWEVCLTIS-EKGFQQVSFVNSIATTKGGRHVDYVVD 329
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Match: TOP2 (Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant [Source:SGD;Acc:S000005032]) HSP 1 Score: 1004.97 bits (2597), Expect = 0.000e+0 Identity = 526/1065 (49.39%), Postives = 719/1065 (67.51%), Query Frame = 2
Query: 122 YQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAK-EDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHV-----LDANEEPLK------VVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLK-HNFVEQFITPIVKAF---KNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKL 3268
YQK +QLEHIL RPD+Y+GS++ +++L W++D+E + M+EK +T P L+KIFDEILVNA+DNK+RD M I ++I ++ + + + N+G+GIP+ IH + +YIP MIFG LLTS+N++DD+K+VTGGRNGYGAKLCNIFS+EF+LET+ G+ + Q W NNM+ C P+I+ K +T VTF PDL RF M LD DI+ + RR YDI + I V+LNGK + ++ FK+YV+LY K + LD E+ ++YE RWEVA S + +F Q+SFVNSIATT GGTHVNY+ DQ+ KI E KKK K ++ Q+K NM+IF+ CLIENP+F SQTKE +T KDFGS C + ++I+ + K + ++ N+ KK T+++++ + PKL+DAN AGTK +CTL+LTEGDSA SLAV+GLAV+GRDYYG +PLRGK+LNVREA Q+ +NAEI I KI+GLQ+ +KYE K+LRYG LMIMTDQD DGSHIKGL+INF+ S +P LL F+ +FITPI+K ++FY++P+ +W+ W+ KYYKGLGTS ++E +EYFS+++RHL F D I LAFSK KAD+RKEWL R+ E G + K I +FIN+ELILFS DN RSIP ++DGFKPGQRKVL+ C K+NL ELKVAQLA V+E +AYHHGE SL TII LAQ+FVGSNNI LL+PNG FGTR GGKD+A+ RYI T+L+KLT+ IFH D+ + KY+ +D +++EPEWY PI+PM+L+NG +GIGTGWS+ IP FNP ++I N++ ++N +MHPW+R +TGTI E+ +Y + G I Q+ LEITELP R WT++ KE ++ + GNDK +I D +E + D ++F++ ++P + A+ GF++ FKL +P++L NM+ FD G I+KYN+ EIL F+ VRLE Y KRK+++ L + K Q +FI I ++ N +I +L
Sbjct: 12 YQKISQLEHILKRPDTYIGSVETQEQLQWIYDEETDCMIEKNVTIVPGLFKIFDEILVNAADNKVRDPSMKRIDVNIHAEEHTIEVKNDGKGIPIEIHNKENIYIPEMIFGHLLTSSNYDDDEKKVTGGRNGYGAKLCNIFSTEFILETADLNVGQKYVQKWENNMSICHPPKITSYKKGPSYTKVTFKPDLTRFGMKELDNDILGVMRRRVYDINGSVRDINVYLNGKSLKIRNFKNYVELYLKSLEKKRQLDNGEDGAAKSDIPTILYERINNRWEVAFAVS-DISFQQISFVNSIATTMGGTHVNYITDQIVKKISEILKKKKKKSVKSF-----QIKNNMFIFINCLIENPAFTSQTKEQLTTRVKDFGSRCEIPLEYINKIMKTDLATRM-FEIADANEENALKKSDGTRKSRITNYPKLEDANKAGTKEGYKCTLVLTEGDSALSLAVAGLAVVGRDYYGCYPLRGKMLNVREASADQILKNAEIQAIKKIMGLQHRKKYE---DTKSLRYGHLMIMTDQDHDGSHIKGLIINFLESSFPGLLDIQGFLLEFITPIIKVSITKPTKNTIAFYNMPDYEKWREEESHKFTWKQKYYKGLGTSLAQEVREYFSNLDRHLKIFHSLQGNDKDYIDLAFSKKKADDRKEWL--------RQYEPGT----VLDPTLKEIPISDFINKELILFSLADNIRSIPNVLDGFKPGQRKVLYGCFKKNLKSELKVAQLAPYVSECTAYHHGEQSLAQTIIGLAQNFVGSNNIYLLLPNGAFGTRATGGKDAAAARYIYTELNKLTRKIFHPADDPLYKYIQEDEKTVEPEWYLPILPMILVNGAEGIGTGWSTYIPPFNPLEIIKNIRHLMNDEELEQMHPWFRGWTGTIEEIEPLRYRMYGRIEQIGDNVLEITELPARTWTSTIKEYLL-LGLSGNDKIKPWIKDMEEQHDD-NIKFIITLSPEEMAKTRKIGFYERFKLISPISLMNMVAFDPHGKIKKYNSVNEILSEFYYVRLEYYQKRKDHMSERLQWEVEKYSFQVKFIKMIIEKELTVTNKPRNAIIQEL 1052
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44813 (DNA topoisomerase 2 [Source:UniProtKB/TrEMBL;Acc:A7RUU5]) HSP 1 Score: 549.28 bits (1414), Expect = 2.713e-180 Identity = 280/484 (57.85%), Postives = 364/484 (75.21%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISK-AAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLK------VVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQL 1540
+E++Y KKTQLEHILLRPD+Y+GS++ + M+V+DD +V ++ITY P L+KIFDEILVNA+DNK RD M+TI+I+I+ + N + +WN+GRGIP+ +HKEHQVY+PTMIFGQLLTS+N+NDD+K+VTGGRNGYGAKLCNIFS +F +ET+ E K FKQ W+NNM +P I + + +++TC+TF PDL++F M LD D VAL +RRAYDIAA +G++V LNGK++ VKTFKDYV L K EE + VV+E RWEV VT S ++ F Q SFVNSIATTKGGTHVNY+ D + + K +K + +KP Q+K ++W+F+ CLIENP+FDSQTKENMTL KDFGS+C L++KFI V G++E + W FK +A L+KKCS +K +K++ +PKLDDAN AG KNS CTLILTEGDSAK+LA+SGL+++GRD YGVFPLRGKLLNVREA KQ+
Sbjct: 37 VERIYTKKTQLEHILLRPDTYIGSVEPNKQNMFVYDDILEAIVNREITYVPGLFKIFDEILVNAADNKQRDPAMSTIKIEINPEKNFISVWNDGRGIPIEMHKEHQVYVPTMIFGQLLTSSNYNDDEKKVTGGRNGYGAKLCNIFSKKFSVETACLENKKRFKQVWTNNMGKAAEPVIKEFTSNKEYTCITFQPDLEKFKMETLDRDTVALLTRRAYDIAASVRGVSVILNGKKLKVKTFKDYVQLCLKGATKDKEEDDEEEKKPTVVHEVVNNRWEVCVTVS-DKGFQQASFVNSIATTKGGTHVNYVTD---QIVDKLMTVIKKKNKGGLAIKPFQIKSHLWVFINCLIENPAFDSQTKENMTLRAKDFGSKCELSDKFIKAVQNSGVMENVMNWMRFKEKAQLNKKCSGSKHSKIRGIPKLDDANDAGGKNSRHCTLILTEGDSAKTLAISGLSIVGRDRYGVFPLRGKLLNVREASHKQV 516
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44811 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU3]) HSP 1 Score: 340.117 bits (871), Expect = 6.880e-105 Identity = 180/370 (48.65%), Postives = 244/370 (65.95%), Query Frame = 2
Query: 2465 LSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKL---TXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVV 3565
LS ++K++F DE LK+L +DNQ IEPEWY PIIP +L+NG +GIGTG+S+K+PN++P+ + +N+++M+NG P +M PWY+NF GTI +V + KY V G I +D TTLEITELP+ WT SYKESV+E HG DK P ITDYKEY+T+ VRF++ M K EAE G HK FKL+ + SNM+LFD G ++KY+ EIL+ FF +R+E YI RK Y+ G+L+A++ KL +QARFILEKI G ++ EN LI LV Y SDPVKAW L + +G PDF YL M + +L+ EKK+ LLK+RD+K EL ++ KSP+ +W +DL+I E+ V
Sbjct: 2 LSTVSKMLFPPSDEPNLKFLNEDNQRIEPEWYCPIIPTILVNGAEGIGTGYSTKVPNYDPRAIAENIRKMMNGEEPEEMLPWYKNFIGTITKVDDTKYSVTGNIEVIDDTTLEITELPIGTWTQSYKESVLEPMLHGTDKVPPCITDYKEYHTETRVRFVITMAENKMREAEQVGLHKKFKLEATINTSNMVLFDAMGCLKKYDTTREILKEFFTLRMERYIMRKAYMEGMLTAESGKLNSQARFILEKIEGTIVIENKPKRDLITMLVQRGYPSDPVKAWKDSQDTTLGVDDSDDDTMSTVSSTASGAGGPDFNYLLNMSMWSLSQEKKDELLKQRDQKAAELEDLRKKSPKDLWNEDLDIFLAELEAV 371
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO29547 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7T3B9]) HSP 1 Score: 233.032 bits (593), Expect = 1.989e-70 Identity = 109/170 (64.12%), Postives = 134/170 (78.82%), Query Frame = 2
Query: 1820 LVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL 2329
+ EW+ T + W++KYYKGLGTST KEAKEYFSDMERH I F+Y G D ++IV+AFSK K ERK+WLT +E+ K RRE G+ E+YLY K TK +++ +F+N+ELILFSNTDNERSIP L+DG KPGQRKV+F C KRN +E+KVAQLAGS+AELSAYHHGE
Sbjct: 1 MEEWKAGTANHKSWKVKYYKGLGTSTPKEAKEYFSDMERHKINFKYEGPHDDEAIVMAFSKKKIQERKDWLTRGLEERKWRREQGLSELYLYEKDTKRVSYCDFVNKELILFSNTDNERSIPSLVDGLKPGQRKVMFTCFKRNDKREVKVAQLAGSIAELSAYHHGEVGF 170
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO44812 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RUU4]) HSP 1 Score: 161.384 bits (407), Expect = 3.720e-46 Identity = 76/113 (67.26%), Postives = 88/113 (77.88%), Query Frame = 2
Query: 1544 ENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKG 1882
+NAEI NIIKI GLQY++KY+ + LK+LRYG LMIMTDQDQDGSHIKGL+INFIHS WP LLK FVEQFITPIVK K + SFYSIPE EW+ + W++KYYKG
Sbjct: 2 DNAEINNIIKITGLQYNKKYKSEDDLKSLRYGHLMIMTDQDQDGSHIKGLVINFIHSNWPGLLKLGFVEQFITPIVKVSKGKEEHSFYSIPEYEEWKAGNANHKSWKVKYYKG 114
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Match: EDO25698 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A8DVR1]) HSP 1 Score: 80.4925 bits (197), Expect = 2.636e-16 Identity = 56/181 (30.94%), Postives = 86/181 (47.51%), Query Frame = 2
Query: 1388 NSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPES--LKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPD------SNKWRLKYYKGLGTSTSKE 1906
+ QC + L EGDSA A G R++ + PLRGK+LNV +A ++ EN EI N+ +G+ + E P + L +RY K++IM D D DGSHI L++ F +++ +V TP + K Q + W N D ++ YKGLG +++
Sbjct: 59 DPAQCEIFLVEGDSAGGTAKQGR---DRNFQAILPLRGKILNVEKAMQHKVFENEEIKNMFTALGVSIGTE-EDPRALNLSKVRYHKVVIMCDADVDGSHIATLILTFFFRYMREMIEQGYV-YIATPPLYLVKKGQKREY-------AWDDNQRDLIAQKMGGGVTIQRYKGLGEMNAEQ 227
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 1385.93 bits (3586), Expect = 0.000e+0 Identity = 711/1200 (59.25%), Postives = 889/1200 (74.08%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFN--ANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKT LEHILLRPD+YVGS++ + MWVFD++ M +++ITY P LYKIFDEILVNA+DNK RD M+ I+I ID + N + IWNNG+GIPV HK+ ++Y+P +IFG LLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KEY SFKQTW NNM +P+I +DFTCVTF PDL +F M +LD D VAL +RRAYD+A +G+ V LNGK++ V F+ YVDLY K LD PLKVV E RWEV +T S + F Q+SFVNSIATTKGG H++Y+ DQ+ AKI+E KKK K P QVK ++W+FV LIENP+FDSQTKENMTL K FGS+C L+EKFI CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE EW++ T + W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F D +LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELPVR WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEA G HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K A + + S P+F Y+ M + LT EK E LLK+RD K EL ++ KS E +W +DL + +E++ V Q+ E G + V K GK K + + ET PS F RR++P
Sbjct: 40 VERVYQKKTPLEHILLRPDTYVGSVEPVSQPMWVFDEDVG-MNQREITYVPGLYKIFDEILVNAADNKQRDKNMSAIKITIDPESNTITIWNNGKGIPVVEHKDEKMYVPALIFGHLLTSSNYDDEEKKVTGGRNGYGAKLCNIFSTKFTVETACKEYRHSFKQTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEKAQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIKLVKGKVGKPKAKKMNL-EETMPSPFGRRVDP 1233
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729]) HSP 1 Score: 1357.04 bits (3511), Expect = 0.000e+0 Identity = 689/1198 (57.51%), Postives = 890/1198 (74.29%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXX--XSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
+E++YQKKTQLEHILLRPDSY+GS++ + MWV+D + + + +T+ P LYKIFDEILVNA+DNK RD M+ I+++ID + N + +WNNG+GIPV HK +VY+P +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KE K+FKQTW +NM D I E+FTC+TF PDL +F M+ LD+D VAL +RRAYD+A ++G+ VF NGK++ + F+ YVD+Y K +D PL V++E+ +RWEV +T S + F QVSFVNSIATTKGG H++Y+ADQ+ +++E KKK K P QVK +MW+FV CL+ENP+FDSQTKENMTL QK FGS C+L++KF CGIVE + W FK Q L+KKCS K TK+K +PKLDDAN AG KNS+ CTLILTEGDSAK+LAVSGL V+GRD YGVFPLRGK+LNVREA KQ+ ENAEI N+IKI+GLQY + Y PESLK+LRYGK+MIMTDQDQDGSHIKGLLINFIH WP+LL+HNF+E+FITPI+KA LSFYSIPE W+ + W++KYYKGLGTSTS+EAKEYFSDMERH I F+YSG ED ++I LAFSK K +ERKEWLTN+M + ++RRE + E YLYG+ TK +++ +FIN+EL+LFSN+DNERSIPCL+DGFKPGQRKVLF C KRN +E+KVAQLAGSVAE+SAYHHGE SLM TI+ LAQ+FVGSNN+NLL P GQFGTRL GGKDSASPRYI T LS L +L+F D+ +LKY DDNQ +EPEWY PIIP VL+NG +GIGTGW+SKIPN++ ++++ N+ RM++G P+ M P Y+ F GTI +V N+Y+ +GE+ +D TT+EI+ELPV+ WT +YKE+V+E +G DK PA ITDYKEY+TD TVRF+V+M K EAEAAG HK FKLQ+PLT ++M+LFD G ++KY + +IL+ FF +R++ Y+ RK +L+G+L A++SKL NQARFILEKI G ++ EN LI L YDSDPV+AW + +AE+E++E A S D+ YL M + LT EKKE L K+RD K+ EL +K KSP +W +DL +E+ + ++ + + K+P K G K +K+ ET P+ RR+ P
Sbjct: 26 VERIYQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLSKFKMSTLDKDTVALMTRRAYDVAGSSKGVKVFFNGKRLPITGFRSYVDMYVKDKVDELGGPLTVIHEAVNERWEVCLTMS-EKGFQQVSFVNSIATTKGGRHIDYVADQIVGRLIEVVKKKNKAGVAVK---PFQVKNHMWLFVNCLVENPTFDSQTKENMTLQQKSFGSTCSLSDKFFKQATSCGIVESIMNWVKFKAQTQLNKKCSAVKHTKIKGVPKLDDANDAGGKNSIGCTLILTEGDSAKTLAVSGLGVVGRDRYGVFPLRGKMLNVREASHKQIMENAEINNVIKILGLQYKKNYSDPESLKSLRYGKIMIMTDQDQDGSHIKGLLINFIHHNWPSLLRHNFLEEFITPIIKASNKKNQLSFYSIPEFNAWKETQSNHKSWKIKYYKGLGTSTSQEAKEYFSDMERHRIPFKYSGPEDDEAISLAFSKKKVEERKEWLTNFMVNRRQRREHNLPEDYLYGQSTKSLSYNDFINKELVLFSNSDNERSIPCLVDGFKPGQRKVLFCCFKRNDKREVKVAQLAGSVAEMSAYHHGEVSLMMTIVGLAQNFVGSNNLNLLQPLGQFGTRLHGGKDSASPRYIFTMLSPLARLVFPPVDDNLLKYNYDDNQRVEPEWYIPIIPTVLVNGAEGIGTGWASKIPNYDIREIVSNIHRMLDGEEPLPMLPNYKGFRGTIDQVMSNQYMNSGEVAIIDSTTIEISELPVKTWTQAYKENVLEPMLNGTDKVPALITDYKEYHTDATVRFVVKMTEEKLMEAEAAGLHKVFKLQSPLTCNSMVLFDYAGSLKKYESVQDILKDFFDLRMKYYVLRKEWLVGMLGAESSKLSNQARFILEKIQGTLVIENKPKKELIRMLQEMGYDSDPVRAWKEAQKKDEDDMEAEEEQEEGAEREDQSSGADYNYLLSMPMWFLTKEKKEDLCKQRDAKMTELNTLKGKSPADLWKEDLAAFSEELERLEQKEKDD----ASAPAKRPAK-GKKPMKVKQETLPTPQGRRVIP 1213
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 1167.14 bits (3018), Expect = 0.000e+0 Identity = 610/1037 (58.82%), Postives = 755/1037 (72.81%), Query Frame = 2
Query: 599 QTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFN--ANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSE--PGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEP 3697
QTW NNM +P+I +DFTCVTF PDL +F M +LD D VAL +RRAYD+A +G+ V LNGK++ V F+ YVDLY K LD PLKVV E RWEV +T S + F Q+SFVNSIATTKGG H++Y+ DQ+ AKI+E KKK K P QVK ++W+FV LIENP+FDSQTKENMTL K FGS+C L+EKFI CGIVE + W FK Q L+KKCS K +K+K +PKLDDAN AG K+S +CTLILTEGDSAKSLAVSGL VIGRD YGVFPLRGK+LNVREA KQ+ ENAEI NIIKI+GLQY + Y+ PESL++LRYGK+MIMTDQDQDGSHIKGLLINF H WP+LLKH F+EQFITPIVKA KN Q LSFYS+PE EW++ T + W +KYYKGLGTSTSKEAKEYF+DMERH I FRY G ED +I LAFSK K D+RKEWLTN+MED ++RR G+ E YLYG TK++++ +FIN+ELILFSN+DNERSIP L+DG KPGQRKVLF CLKRN +E+KVAQLAGSVAE+SAYHHGE +LM TI+NLAQ+FVGSNNIN+L P GQFGTR+ GGKD+ASPRYI T LS LTKL+F D +LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELPVR WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEA G HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K A + + S P+F Y+ M + LT EK E LLK+RD K EL ++ KS E +W +DL + +E++ V Q+ E G + V K GK K + + ET PS F RR++P
Sbjct: 123 QTWQNNMTKTSEPKIRFFDGDDFTCVTFQPDLAKFKMEKLDRDTVALMTRRAYDVAGSCRGVKVTLNGKKLPVNGFRSYVDLYVKEKLDETGVPLKVVNEMVNDRWEVCLTMS-EKGFQQISFVNSIATTKGGRHIDYVVDQIVAKIIEVVKKKNKAGVTVK---PFQVKNHIWVFVNALIENPTFDSQTKENMTLQTKSFGSKCVLSEKFIRAATNCGIVESILNWVKFKAQTQLNKKCSSVKHSKIKGIPKLDDANDAGGKHSSECTLILTEGDSAKSLAVSGLGVIGRDRYGVFPLRGKILNVREATHKQIMENAEINNIIKIVGLQYKKSYDDPESLRSLRYGKIMIMTDQDQDGSHIKGLLINFFHHNWPSLLKHTFLEQFITPIVKATKNKQELSFYSLPEFEEWKKQTDNYKSWHVKYYKGLGTSTSKEAKEYFADMERHRITFRYQGAEDDAAITLAFSKKKTDDRKEWLTNFMEDRRQRRMHGLPEKYLYGTQTKHLSYNDFINKELILFSNSDNERSIPSLVDGLKPGQRKVLFTCLKRNDKREVKVAQLAGSVAEMSAYHHGEQALMMTIVNLAQNFVGSNNINILQPLGQFGTRINGGKDAASPRYIFTMLSPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEKAQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDNVEAQEREEQSAGRAIKLVKGKVGKPKAKKMNL-EETMPSPFGRRVDP 1154
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2b (DNA topoisomerase II beta [Source:NCBI gene;Acc:101157229]) HSP 1 Score: 340.887 bits (873), Expect = 7.716e-105 Identity = 183/370 (49.46%), Postives = 252/370 (68.11%), Query Frame = 2
Query: 2468 SKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFN--ANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYN 3571
S LTKL+F D +LK+L DDNQ +EPEWY PIIPMVL+NG +GIGTGW+ KIPN++P+++++N+ RM+N P+ M P Y+NF G I E+ +N+Y+V+GE++ LD T+EITELPVR WT +YKESV+E G DKTPA I DYKEY+TD TV+F+V M+ K A+AEA G HK FKLQ+ LT ++M+LFD G +++Y++ +IL+ FF++RL RK++LLG L A+A+KL NQARF+LEKI GK+ EN LI LV Y+SDPV AW K A + + S P+F Y+ M + LT EK E LLK+RD K EL ++ KS E +W +DL + +E++V ++
Sbjct: 5 SPLTKLLFPAVDSNLLKFLYDDNQKVEPEWYIPIIPMVLVNGAEGIGTGWACKIPNYDPREIVNNINRMLNHQDPLPMLPSYKNFKGVIHELGQNQYLVSGEVSVLDKNTIEITELPVRTWTQAYKESVLEPMLQGTDKTPALINDYKEYHTDSTVKFVVRMSEEKLAQAEAVGLHKVFKLQSSLTCNSMVLFDHMGCLKRYDSVQDILKEFFELRLHYCKLRKDWLLGSLGAEAAKLSNQARFVLEKIEGKISIENKSKRELIRMLVQKGYESDPVAAWSKAQEKAQEEGETDGNQSDSSVDSGSSSGPNFNYILNMPLWCLTKEKVEELLKQRDIKRGELADLQKKSSEDLWKEDLAVFIEELDVSFS 374
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Match: top2a (DNA topoisomerase II alpha [Source:NCBI gene;Acc:101158729]) HSP 1 Score: 249.98 bits (637), Expect = 4.519e-75 Identity = 114/196 (58.16%), Postives = 153/196 (78.06%), Query Frame = 2
Query: 110 IEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLK 697
+E++YQKKTQLEHILLRPDSY+GS++ + MWV+D + + + +T+ P LYKIFDEILVNA+DNK RD M+ I+++ID + N + +WNNG+GIPV HK +VY+P +IFGQLLTS+N++D++K+VTGGRNGYGAKLCNIFS++F +ET+ KE K+FKQTW +NM D I E+FTC+TF PDLK
Sbjct: 20 VERIYQKKTQLEHILLRPDSYIGSVEPVTQQMWVYDVDVG-LNCRDVTFVPGLYKIFDEILVNAADNKQRDKSMSCIKVNIDVENNTISVWNNGKGIPVVEHKVEKVYVPALIFGQLLTSSNYDDEQKKVTGGRNGYGAKLCNIFSTKFTVETACKESRKTFKQTWYDNMGRAGDTNIKAFDGEEFTCITFKPDLK 214
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2356.64 bits (6106), Expect = 0.000e+0 Identity = 1386/1557 (89.02%), Postives = 1445/1557 (92.81%), Query Frame = 2
Query: 77 DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEPXXXXXXXXXXXXXXXXXXXXXXXXXQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVXXXXXXXXXXXXXXXXXXXXXXXXXXFESXXXXXXXXXXXXXXXXXXXREFDAIPRETVLKRNAAKNVKYXXXXXXXXXXXXXXXXXXXXXXXXXXXYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKIEDISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKVDYXXXXXXXXXXXFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPQKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 4747
D+SL+ E IEK Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+ NM+RIWNNG+GIPV +HK+ V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM DP IS KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL +WYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELP+RVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW K + KL +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGK+GGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKK ETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDT+REFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEK+ DIS ++K I P AKKLKTNKPKTTTNAASNKKKEKQKVD KTKKAK PRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIP+KVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE
Sbjct: 4 DDSLSLEENESIEKRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQL----------------------------KWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDTSREFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKMVDIS-KPAKRKKIIKDKDEPPAKKLKTNKPKTTTNAASNKKKEKQKVDCKTKKAKAPRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPKKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 1530
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2342.38 bits (6069), Expect = 0.000e+0 Identity = 1382/1557 (88.76%), Postives = 1446/1557 (92.87%), Query Frame = 2
Query: 77 DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEPXXXXXXXXXXXXXXXXXXXXXXXXXQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVXXXXXXXXXXXXXXXXXXXXXXXXXXFESXXXXXXXXXXXXXXXXXXXREFDAIPRETVLKRNAAKNVKYXXXXXXXXXXXXXXXXXXXXXXXXXXXYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKIEDISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKVDYXXXXXXXXXXXFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPQKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 4747
D+SL+ E IEK Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+ NM+RIWNNG+GIPV +HK+ V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM DP IS KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK W + ++ +G+ + KIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELP+RVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW K + KL +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGK+GGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKK ETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDT+REFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEK+ DIS ++K I P AKKLKTNKPKTTTNAASNKKKEKQKVD KTKKAK PRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIP+KVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE
Sbjct: 4 DDSLSLEENESIEKRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKIC----------WMT-----INLSNQNGMHQLFRCKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDTSREFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKMVDIS-KPAKRKKIIKDKDEPPAKKLKTNKPKTTTNAASNKKKEKQKVDCKTKKAKAPRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPKKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 1543
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037565.1 (SMESG000037565.1) HSP 1 Score: 2313.49 bits (5994), Expect = 0.000e+0 Identity = 1374/1557 (88.25%), Postives = 1434/1557 (92.10%), Query Frame = 2
Query: 77 DESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEPXXXXXXXXXXXXXXXXXXXXXXXXXQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVXXXXXXXXXXXXXXXXXXXXXXXXXXFESXXXXXXXXXXXXXXXXXXXREFDAIPRETVLKRNAAKNVKYXXXXXXXXXXXXXXXXXXXXXXXXXXXYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKIEDISXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXQKVDYXXXXXXXXXXXFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPQKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 4747
D+SL+ E IEK Y KKTQLEHILLRPDSYVGSIK E+K +W+FD EKN MVEK+ITYNP LYKI+DEILVNASDNKIRD KM+ IRIDI+ NM+RIWNNG+GIPV +HK+ V++PTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFS+EF+LETS+KEYGKSFKQTW+ NM DP IS KEDFTCVTFYPDLKRF+M+ LD++ +ALFSRRAYDIAACT GI V+LNG+QI VK FKDYV+LYTK+ LD+NEE LKV+YE +G RWEVA+TTS+N+ F QVSFVNSIATTKGGTHVN++ DQ A K+LE AKKKIK DKQAVKLK AQVKMNMW+FV+CLIENPSFDSQTKENMTLN KDFGS C L +KFIS VAKCGIVEK+TMW +FKNQALLDKKCSKTK TKLKD+PKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK I + IN + N N DNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELP+RVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAW K + KL +EE++E IEDS KPDFGYLTQMQILTLTAEKK+ALLKERDEKL+EL I+NKSP+QMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGK+GGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKK ETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDT+REFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEK+ DIS ++K I P AKKLKTNKPKTTTNAASNKKKEKQKVD KTKKAK PRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIP+KVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE
Sbjct: 4 DDSLSLEENESIEKRYLKKTQLEHILLRPDSYVGSIKPEEKALWIFDSEKNCMVEKRITYNPGLYKIYDEILVNASDNKIRDRKMSCIRIDINGPKNMIRIWNNGKGIPVVMHKDVNVHVPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSNEFILETSNKEYGKSFKQTWTKNMGVASDPIISSCQKEDFTCVTFYPDLKRFNMSHLDDETIALFSRRAYDIAACTNGIVVYLNGQQIPVKNFKDYVELYTKNCLDSNEEELKVIYEKFGDRWEVAMTTSLNDNFCQVSFVNSIATTKGGTHVNHITDQAAVKLLEIAKKKIKSDKQAVKLKVAQVKMNMWVFVKCLIENPSFDSQTKENMTLNAKDFGSTCALTDKFISQVAKCGIVEKITMWAMFKNQALLDKKCSKTKHTKLKDIPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLK-----------------ICWMTIN------------LSNQN-----DNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWQK-SVEKLLAIDKGEEENQEDIEDSNKPDFGYLTQMQILTLTAEKKDALLKERDEKLKELDIIRNKSPQQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKLTTETEKKKKKTETKKQNQIIVKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPVTTKELNKLKSNKKKNSTSPNKKRKRNFESDTDDYDDINDDLEEDIDTSREFDAIPRETVLKRNAAKNVKYNLNDDDDDEEANEIDKSSKSSLSIDDLYDPSLESPIISKGNTQPVAINKKVEIPNNDVFSIDSEKMVDIS-KPAKRKKIIKDKDEPPAKKLKTNKPKTTTNAASNKKKEKQKVDCKTKKAKAPRKKFSSDDEADSIFKPDDDSASDAEVGTQRARRKIPKKVYIDSDGDDDEFGGMADDIFSQDKTKPKRMKGGDSSGSE 1524
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1) HSP 1 Score: 2242.62 bits (5810), Expect = 0.000e+0 Identity = 1215/1296 (93.75%), Postives = 1234/1296 (95.22%), Query Frame = 2
Query: 59 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEPXXXXXXXXXXXXXXXXXXXXXXXXXQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPV 3937
MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAG K+ H AVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELP+RVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGK+GGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+ ETEKKKK +E KKQ ++I V + DN +S + EE LAQRL+ + +EAFK L+ K KP
Sbjct: 1 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGA-----------------KIALHLVTYAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPT 1278
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Match: SMESG000037559.1 (SMESG000037559.1) HSP 1 Score: 2226.05 bits (5767), Expect = 0.000e+0 Identity = 1208/1296 (93.21%), Postives = 1227/1296 (94.68%), Query Frame = 2
Query: 59 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADXXXXXXXXXXXXXXXXXXXXXXXXPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALXXXXXXXXXXXXXXXMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLRGKLLNVREAGVKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQRNTPDSNKWRLKYYKGLGTSTSKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLMSTIINLAQDFVGSNNINLLMPNGQFGTRLAGGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPVRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGKVICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTXXXXXXXXXXXXXXXSGKPDFGYLTQMQIXXXXXXXXXXXXXXXXXXXXXXMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKRGGKAIKIAAETAPSKFSRRIEPXXXXXXXXXXXXXXXXXXXXXXXXXQNQII---VKENGDIVTTNSRDNIDSVDYEEAPLAQRLSNANSNEAFKDLVRKIKKPV 3937
MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPL+GKLLNVREA KQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQ+ TP +N+WRLKYYKGLGTST KEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASL+ GKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELP+RVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKING +ICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGK+GGKAIKIAAETAPSKFSRRIEPKIEEEYLKK+ ETEKKKK +E KKQ ++I V + DN +S + EE LAQRL+ + +EAFK L+ K KP
Sbjct: 1 MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWVFDDEKNEMVEKKITYNPALYKIFDEILVNASDNKIRDSKMTTIRIDIDSKMNMVRIWNNGRGIPVTIHKEHQVYIPTMIFGQLLTSTNFNDDKKQVTGGRNGYGAKLCNIFSSEFVLETSSKEYGKSFKQTWSNNMNDCFDPEISKAAKEDFTCVTFYPDLKRFSMTRLDEDIVALFSRRAYDIAACTQGITVFLNGKQINVKTFKDYVDLYTKHVLDANEEPLKVVYESYGKRWEVAVTTSVNETFSQVSFVNSIATTKGGTHVNYLADQLAAKILEKAKKKIKDDKQAVKLKPAQVKMNMWIFVRCLIENPSFDSQTKENMTLNQKDFGSECTLNEKFISLVAKCGIVEKLTMWTLFKNQALLDKKCSKTKQTKLKDMPKLDDANFAGTKNSLQCTLILTEGDSAKSLAVSGLAVIGRDYYGVFPLKGKLLNVREASAKQLTENAEITNIIKIIGLQYSQKYELPESLKTLRYGKLMIMTDQDQDGSHIKGLLINFIHSKWPNLLKHNFVEQFITPIVKAFKNNQVLSFYSIPELVEWQKVTPGANQWRLKYYKGLGTSTDKEAKEYFSDMERHLIKFRYSGEEDGKSIVLAFSKTKADERKEWLTNWMEDCKRRRELGMDEVYLYGKGTKYITFENFINQELILFSNTDNERSIPCLMDGFKPGQRKVLFACLKRNLVKELKVAQLAGSVAELSAYHHGEASLI------------------------------WGKDSASPRYICTQLSKLTKLIFHHDDEAVLKYLLDDNQSIEPEWYAPIIPMVLINGCDGIGTGWSSKIPNFNPKDVIDNLKRMINGGTPVKMHPWYRNFTGTIVEVCENKYVVNGEINQLDGTTLEITELPIRVWTNSYKESVMEIYRHGNDKTPAFITDYKEYNTDKTVRFLVEMNPAKYAEAEAAGFHKFFKLQTPLTLSNMILFDECGVIRKYNNGIEILESFFKVRLEIYIKRKNYLLGILSAQASKLQNQARFILEKINGIIICENVKIAVLIDKLVAAKYDSDPVKAWMKFNANKLTIEKAEDEEDEEAIEDSGKPDFGYLTQMQILTLTAEKKEALLKERDEKLEELMRIKNKSPEQMWLDDLEILEKEINVVYNQDTSEPGEIVNTVVKKPGKKGGKAIKIAAETAPSKFSRRIEPKIEEEYLKKINLETEKKKKLVENKKQKEMIENFVTRESTGTNAEAGDN-NSENDEELTLAQRLAKTHGSEAFKGLIGKGDKPT 1265 The following BLAST results are available for this feature:
BLAST of DNA topoisomerase 2 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 4
BLAST of DNA topoisomerase 2 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 5
BLAST of DNA topoisomerase 2 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 3
BLAST of DNA topoisomerase 2 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 2
BLAST of DNA topoisomerase 2 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 1
BLAST of DNA topoisomerase 2 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 5
BLAST of DNA topoisomerase 2 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010418 ID=SMED30010418|Name=DNA topoisomerase 2|organism=Schmidtea mediterranea sexual|type=transcript|length=4810bpback to top protein sequence of SMED30010418-orf-1 >SMED30010418-orf-1 ID=SMED30010418-orf-1|Name=SMED30010418-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1564bp MQSSLLDESLNERVPEDIEKLYQKKTQLEHILLRPDSYVGSIKKEDKLMWback to top Annotated Terms
The following terms have been associated with this transcript:
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