pmp-10
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010415 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 15Note: Hover over icons to view figure legend
Homology
BLAST of pmp-10 vs. TrEMBL
Match: A0A0A1CQX9 (Pmp-10 (Fragment) OS=Schmidtea mediterranea OX=79327 GN=pmp-10 PE=2 SV=1) HSP 1 Score: 520.005 bits (1338), Expect = 0.000e+0 Identity = 260/262 (99.24%), Postives = 260/262 (99.24%), Query Frame = 1 Query: 298 NRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNDENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKKPTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSN 1083 NRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFN ENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKK TRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSN Sbjct: 1 NRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNVENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKKTTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSN 262
BLAST of pmp-10 vs. Planmine SMEST
Match: SMESG000068428.1 (SMESG000068428.1) HSP 1 Score: 630.943 bits (1626), Expect = 0.000e+0 Identity = 335/345 (97.10%), Postives = 340/345 (98.55%), Query Frame = 1 Query: 244 MKPEXXXXXXXXXYEIHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNDENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKKPTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSNXXXXXXXXXXXXXRGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQR 1278 MKPE LLLFIFIFYEIHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFN ENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKK TRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSNELDDGDEEEDILERGLT+RKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNEF+ E E+DF++R Sbjct: 1 MKPEFLLLFIFIFYEIHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNVENKIQYLKSLTNEKDKMEYVQKRLQTKKPLKKTTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSNELDDGDEEEDILERGLTQRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNEFDEEAEDDFMKR 345 HSP 2 Score: 96.6709 bits (239), Expect = 1.028e-20 Identity = 74/122 (60.66%), Postives = 94/122 (77.05%), Query Frame = 1 Query: 883 KSLTNE---KDKMEYVQKRLQTKKPLKKPTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSNXXXXXXXXXXXXXRGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISN 1239 +S++NE +D+ ++++ + +K P K R + KV T+KP T K++ V+ TKS++KS +TP S+ELDDGDEEEDILERG TKRKSTTVKPATHTKK GTIKPIIPTKKVGKRSISN Sbjct: 430 RSISNEFEAEDEDNFMKRAVTSKHPKTK-KRNKPVTSKVLTVKPTKYTPKTVTVKPTKSISKSTQTPRSDELDDGDEEEDILERGFTKRKSTTVKPATHTKKVGTIKPIIPTKKVGKRSISN 550 HSP 3 Score: 96.6709 bits (239), Expect = 1.028e-20 Identity = 74/122 (60.66%), Postives = 94/122 (77.05%), Query Frame = 1 Query: 883 KSLTNE---KDKMEYVQKRLQTKKPLKKPTRKHSLIIKVSTMKPRTQTTKSIKVRSTKSVNKSEKTPSSNXXXXXXXXXXXXXRGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISN 1239 +S++NE +D+ ++++ + +K P K R + KV T+KP T K++ V+ TKS++KS +TP S+ELDDGDEEEDILERG TKRKSTTVKPATHTKK GTIKPIIPTKKVGKRSISN Sbjct: 852 RSISNEFEAEDEDNFMKRAVTSKHPKTK-KRNKPVTSKVLTVKPTKYTPKTVTVKPTKSISKSTQTPRSDELDDGDEEEDILERGFTKRKSTTVKPATHTKKVGTIKPIIPTKKVGKRSISN 972 HSP 4 Score: 81.2629 bits (199), Expect = 6.844e-16 Identity = 47/53 (88.68%), Postives = 51/53 (96.23%), Query Frame = 1 Query: 1123 RGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQRM 1281 R +TK+KSTTVKPATHTKK GTIKPIIPTKKVGKRSISNEFEA+EEEDF+QRM Sbjct: 1087 RAVTKKKSTTVKPATHTKKVGTIKPIIPTKKVGKRSISNEFEAQEEEDFLQRM 1139 HSP 5 Score: 74.3294 bits (181), Expect = 1.074e-13 Identity = 41/52 (78.85%), Postives = 48/52 (92.31%), Query Frame = 1 Query: 1123 RGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQR 1278 R +TK+K+TTVKPATHTKK GTIKPIIPTKKVGKRSISNEF+ E E+DF++R Sbjct: 665 RAVTKKKNTTVKPATHTKKVGTIKPIIPTKKVGKRSISNEFDEEAEDDFMKR 716 HSP 6 Score: 74.3294 bits (181), Expect = 1.074e-13 Identity = 41/52 (78.85%), Postives = 48/52 (92.31%), Query Frame = 1 Query: 1123 RGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQR 1278 R +TK+K+TTVKPATHTKK GTIKPIIPTKKVGKRSISNEF+ E E+DF++R Sbjct: 716 RAVTKKKNTTVKPATHTKKVGTIKPIIPTKKVGKRSISNEFDEEAEDDFMKR 767 HSP 7 Score: 73.9442 bits (180), Expect = 1.602e-13 Identity = 43/52 (82.69%), Postives = 50/52 (96.15%), Query Frame = 1 Query: 1123 RGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQR 1278 R +TK+KSTTVKPATHTKK GTIKPIIPTKKVGKRSISNEFEAE+E++F++R Sbjct: 818 RAVTKKKSTTVKPATHTKKVGTIKPIIPTKKVGKRSISNEFEAEDEDNFMKR 869 HSP 8 Score: 72.0182 bits (175), Expect = 5.403e-13 Identity = 42/52 (80.77%), Postives = 50/52 (96.15%), Query Frame = 1 Query: 1123 RGLTKRKSTTVKPATHTKKAGTIKPIIPTKKVGKRSISNXXXXXXXXDFIQR 1278 R +TK+K+TTVKPATHTKK GTIKPIIPTKKVGKRSISNEFEAE+E++F++R Sbjct: 396 RAVTKKKNTTVKPATHTKKVGTIKPIIPTKKVGKRSISNEFEAEDEDNFMKR 447
BLAST of pmp-10 vs. Planmine SMEST
Match: SMESG000068743.1 (SMESG000068743.1) HSP 1 Score: 261.151 bits (666), Expect = 7.016e-76 Identity = 122/205 (59.51%), Postives = 153/205 (74.63%), Query Frame = 1 Query: 289 IHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYWNEKYKKGEFKISRKNLQIEQLKNIATSEGFFNDENKIQYLKSLTNEK 903 I ++ C+IG+L+VK AK+LI+N+ QR QCN LIPE SCD AY+T+D YC+ KA+ ID C+ F KL E CN AEN IK C K GY+TA CGR F+RLD VCRI S+T SR CTK YRKMDN+C KKY KI +KCKRI+ RYKDFCE+ ++ NYWNE YK GEFKI+ K ++IE +KN+A E FF++ N +Y+K L NEK Sbjct: 16 IQESDCKIGELAVKKAKSLILNKAQREQCNLLIPEADSCDQAYQTVDKYCDEKASSIDKECDISFKKLVEKCNTAENSIKERCQKKNGYVTAECGRLFERLDNVCRIVSSTQSRHCTKLYRKMDNECYKKYSKITLKCKRIFDRYKDFCESDMSPNYWNEAYKDGEFKINEKIIKIESVKNMAEEENFFDEINIKKYVK-LLNEK 219
BLAST of pmp-10 vs. Planmine SMEST
Match: SMESG000043853.1 (SMESG000043853.1) HSP 1 Score: 98.2117 bits (243), Expect = 5.095e-23 Identity = 49/152 (32.24%), Postives = 80/152 (52.63%), Query Frame = 1 Query: 307 EIGDLSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYW 762 EIGDLS+ +++LI ++ C +L+P +CD A +D+YC + + FC +F K CNK E KI C +G++ CG FD L VC++ S S C + ++ + CN KI C+ +Y Y +FC+ +++ Y+ Sbjct: 22 EIGDLSIHESQSLINFKSTHQTCGNLVPITKTCDKALSIVDDYCYSQLHQTIRFCEHQFQKGVYYCNKTEKKITKTCIRNIGFLNEKCGSFFDSLRIVCQLESDYQSVVCDRLLKQNEKNCNNFKNKILRNCRHVYQHYLNFCDFSISDTYY 173
BLAST of pmp-10 vs. Planmine SMEST
Match: SMESG000053861.1 (SMESG000053861.1) HSP 1 Score: 82.0333 bits (201), Expect = 5.297e-18 Identity = 48/138 (34.78%), Postives = 68/138 (49.28%), Query Frame = 1 Query: 370 QCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDR-------LDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGVANNYW 762 +C DLIP CD AY T DNYC+R A+ +D C+ F KLT CNK E GYI +CG++ R LD C + +S C+ +Y M +KC ++ +C IY +Y++ CE + W Sbjct: 30 ECEDLIPFAEHCDRAYLTADNYCDRYASHLDKQCDIAFRKLTNGCNKHE-----------GYILQTCGKKGPRCKVMINFLDITCNQACVAISDYCSDEYDYMTDKCEFINNRLEKRCNGIYQQYEETCEKNLDPYNW 156
BLAST of pmp-10 vs. Planmine SMEST
Match: SMESG000034829.1 (SMESG000034829.1) HSP 1 Score: 68.1662 bits (165), Expect = 4.432e-13 Identity = 42/143 (29.37%), Postives = 67/143 (46.85%), Query Frame = 1 Query: 319 LSVKIAKALIINRNQRLQCNDLIPEVISCDSAYETIDNYCERKATEIDSFCNRKFSKLTESCNKAENKIKLDCNTKVGYITASCGRRFDRLDKVCRISSTTLSRKCTKKYRKMDNKCNKKYGKINVKCKRIYYRYKDFCETGV 747 + V I+ + I N+ +C +L+ +VISCD ++ +D YC K D C R++ L CN K CN + T C + DR+D+ C L C YRK+D CN+ ++ +C I+ Y C+ + Sbjct: 7 IFVWISISTISGNNE--ECQNLLKKVISCDGDWKALDKYCAIKFETHDKVCERRYFYLDTRCNTDYEKYDKVCNYRER--TGKCIKMLDRIDRYCVAKYAILDMNCDSDYRKLDKHCNQGSKELQHRCLAIFSNYHRVCKDEI 145 The following BLAST results are available for this feature:
BLAST of pmp-10 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of pmp-10 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of pmp-10 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of pmp-10 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of pmp-10 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of pmp-10 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of pmp-10 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of pmp-10 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Cross References
External references for this transcript
Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010415 ID=SMED30010415|Name=pmp-10|organism=Schmidtea mediterranea sexual|type=transcript|length=1541bpback to top protein sequence of SMED30010415-orf-1 >SMED30010415-orf-1 ID=SMED30010415-orf-1|Name=SMED30010415-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=347bp MKPELLLLFIFIFYEIHQNRCEIGDLSVKIAKALIINRNQRLQCNDLIPEback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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