SMED30010410

Overview
NameSMED30010410
Smed IDSMED30010410
Length (bp)732
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010410 (SMED30010410) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010410 (SMED30010410) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010410 (SMED30010410) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010410 (SMED30010410) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010410

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 8

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
head regionSMED30010410SMESG000021757.1 SmedASXL_005342SmedAsxl_ww_GCZZ01PMID:27034770
Currie et al., 2016		whole organism asexual adult RNA-sequencing evidence
pharynxSMED30010410SMESG000021757.1 dd_Smed_v4_8797_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
nervous systemSMED30010410SMESG000021757.1 dd_Smed_v4_8797_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010410SMESG000021757.1 dd_Smed_v4_8797_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
head regionSMED30010410SMESG000021757.1 dd_Smed_v6_8797_0_1dd_Smed_v6PMID:28171748
Stückemann et al., 2017		whole organism asexual adult RNA-sequencing evidence
reproductive organSMED30010410SMESG000021757.1 Contig44448newmark_estsPMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
reproductive organSMED30010410SMESG000021757.1 Contig44448uc_Smed_v2PMID:28434803
Rouhana et al., 2017		whole organism adult hermaphrodite RNA-sequencing evidence
epidermisSMED30010410SMESG000021757.1 dd_Smed_v4_8797_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010410 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.000046:254521..255383 +3508
Homology
[Close]
BLAST of SMED30010410 vs. Ensembl Human
Match: C9orf135 (chromosome 9 open reading frame 135 [Source:HGNC Symbol;Acc:HGNC:31422])

HSP 1 Score: 59.3066 bits (142), Expect = 5.677e-10
Identity = 56/197 (28.43%), Postives = 91/197 (46.19%), Query Frame = 1 
Query:   37 ALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQ-RSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELD------KGMPMFSVEAIDSPLFD------ANYNDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTIY 588
             LR     YS+ V   +WH+ RE+ PK +D    E+ + L +STY+ LG   D++P+ T +     +K++ + L+      K   + + E + S + +      A      FPRH   + +    TTY+ DYV PY +Q      +D D +  ++KC SQF    G       R G N  + +  IY   +V   +Y
Sbjct:   34 TLRSHHKKYSKPVLVYSWHRDREAFPKGYDIEGPEKVKKLCNSTYRRLGT--DESPIWTSE---THEKLSQMCLNTEWVEMKSKALLNEETVSSGIIERVTGLPATGFGAVFPRHPPDWSKMCALTTYSEDYVPPYDYQPHAYPCQD-DYSIVHRKCRSQFTDLNG-----SKRFGINTWHDESGIYANSDVKQKLY 219
[Close]
BLAST of SMED30010410 vs. Ensembl Xenopus
Match: smim14 (small integral membrane protein 14 [Source:Xenbase;Acc:XB-GENE-5732314])

HSP 1 Score: 55.0694 bits (131), Expect = 1.207e-8
Identity = 55/193 (28.50%), Postives = 83/193 (43.01%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNT--AEQRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMP--MFSVEAIDSPLFDANYNDDG------FPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTIY 588
            LR +   Y R      WHQ RE+EPKD+D  T    +++L  STY+ LG +      +T ++   Q ++      + +P  M +   ID     AN   D        P+H     +  ++TTY +D  Y +PFQ   I     D + + KKC SQF         D  R G N    +  IY    +  +I+
Sbjct:   18 LRSNHVTYGRPTLVSGWHQNREAEPKDYDLATLPVGKKNLCQSTYRSLGKVEISDWKTTTEDQLSQVQLKKDYEARDVPKSMVNENTID---VVANRQTDHSQRGYVLPQHDKDHGKMHLKTTYYID--YSFPFQPTTIPDVR-DCSAAYKKCHSQF-----TDTADYRRHGRNTWQDESGIYANHQIKHSIF 199
[Close]
BLAST of SMED30010410 vs. UniProt/SwissProt
Match: sp|Q32L77|CI135_BOVIN (Protein C9orf135 homolog OS=Bos taurus OX=9913 PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 1.008e-10
Identity = 58/195 (29.74%), Postives = 89/195 (45.64%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQ-RSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKIN-HLELDKGMPMFSVEAIDSPLFDANYNDDGFPRHRFG--FDRQQME-------TTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTIYT 591
            LR     YS+ V   +WH+ RE+ PKD+D    E+ + L +STY+ LG       +S  +E   Q  +N  L   K   + + E ++S + +    D G P   FG  F R   +       TTYA +Y  PY +Q +    +D D +  ++KC SQF   +G       R G N  + +  IY    V   +Y+
Sbjct:   35 LRSHHKKYSKPVLVYSWHRDRETYPKDYDIEGPEEVKKLCNSTYRRLGTSEPPVWISETREKMAQVCLNTKLAKIKSKALLNEETMNSGIIE---RDTGLPATGFGALFTRHSPDWSKMCTLTTYAEEYAPPYEYQPLGDPCQDDDYSIVHRKCRSQFTDLDG-----SKRVGINTWHDESGIYANSYVKRKLYS 221
[Close]
BLAST of SMED30010410 vs. UniProt/SwissProt
Match: sp|Q5VTT2|CI135_HUMAN (Protein C9orf135 OS=Homo sapiens OX=9606 GN=C9orf135 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 2.726e-9
Identity = 56/197 (28.43%), Postives = 91/197 (46.19%), Query Frame = 1 
Query:   37 ALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQ-RSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELD------KGMPMFSVEAIDSPLFD------ANYNDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTIY 588
             LR     YS+ V   +WH+ RE+ PK +D    E+ + L +STY+ LG   D++P+ T +     +K++ + L+      K   + + E + S + +      A      FPRH   + +    TTY+ DYV PY +Q      +D D +  ++KC SQF    G       R G N  + +  IY   +V   +Y
Sbjct:   34 TLRSHHKKYSKPVLVYSWHRDREAFPKGYDIEGPEKVKKLCNSTYRRLGT--DESPIWTSE---THEKLSQMCLNTEWVEMKSKALLNEETVSSGIIERVTGLPATGFGAVFPRHPPDWSKMCALTTYSEDYVPPYDYQPHAYPCQD-DYSIVHRKCRSQFTDLNG-----SKRFGINTWHDESGIYANSDVKQKLY 219
[Close]
BLAST of SMED30010410 vs. TrEMBL
Match: A0A1I8H061 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030114g1 PE=4 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.389e-20
Identity = 61/173 (35.26%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYL----GGLPDKTPLSTYQEFCQ--QKKINHLELDKGMPMFSVEAIDSPLFDANYNDDG------FPRHRFGFDRQQMETTYAVDYVYPYPFQHI--------EIETKDPD-LTKSNKKCLSQFLIQEG 495
            L G+V++Y R+VKTG WH +RE+EPKD++ +T + R+L  STY  L    G   D T   TY   C   Q K ++ E  K   M + E + S + D +           FPRH   +D+  +E+TY  DY      + I        ++E  D D LT +NK+ LSQF    G
Sbjct:   10 LSGNVANYGRIVKTGKWHLSREAEPKDYEISTDKVRNLKRSTYDRLAAESGAFEDST---TYGSMCDQLQTKGDYTEHPKA-SMVTAETVASVVLDRDIGRPSRGPGALFPRHSSKYDKHHLESTYNADYGDRVTDEQIKQKCESENKVERPDEDLLTAANKRPLSQFCDTAG 178

HSP 2 Score: 43.1282 bits (100), Expect = 1.389e-20
Identity = 17/30 (56.67%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  477 ISDPGGYNRPGHNTWWDESGIYANSHLHRQ 566
              D  G+ R G NTW DE+G+YAN+HL RQ
Sbjct:  173 FCDTAGHRRQGRNTWQDETGVYANTHLRRQ 202
[Close]
BLAST of SMED30010410 vs. TrEMBL
Match: A0A267DCI9 (Uncharacterized protein (Fragment) OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig030114g2 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.679e-20
Identity = 61/173 (35.26%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYL----GGLPDKTPLSTYQEFCQ--QKKINHLELDKGMPMFSVEAIDSPLFDANYNDDG------FPRHRFGFDRQQMETTYAVDYVYPYPFQHI--------EIETKDPD-LTKSNKKCLSQFLIQEG 495
            L G+V++Y R+VKTG WH +RE+EPKD++ +T + R+L  STY  L    G   D T   TY   C   Q K ++ E  K   M + E + S + D +           FPRH   +D+  +E+TY  DY      + I        ++E  D D LT +NK+ LSQF    G
Sbjct:    9 LSGNVANYGRIVKTGKWHLSREAEPKDYEISTDKVRNLKRSTYDRLAAESGAFEDST---TYGSMCDQLQTKGDYTEHPKA-SMVTAETVASVVLDRDIGRPSRGPGALFPRHSSKYDKHHLESTYNADYGDRVTDEQIKQKCESENKVERPDEDLLTAANKRPLSQFCDTAG 177

HSP 2 Score: 43.1282 bits (100), Expect = 1.679e-20
Identity = 17/30 (56.67%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  477 ISDPGGYNRPGHNTWWDESGIYANSHLHRQ 566
              D  G+ R G NTW DE+G+YAN+HL RQ
Sbjct:  172 FCDTAGHRRQGRNTWQDETGVYANTHLRRQ 201
[Close]
BLAST of SMED30010410 vs. TrEMBL
Match: A0A267GMR9 (Uncharacterized protein OS=Macrostomum lignano OX=282301 GN=BOX15_Mlig006242g1 PE=4 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 1.884e-20
Identity = 61/173 (35.26%), Postives = 87/173 (50.29%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYL----GGLPDKTPLSTYQEFCQ--QKKINHLELDKGMPMFSVEAIDSPLFDANYNDDG------FPRHRFGFDRQQMETTYAVDYVYPYPFQHI--------EIETKDPD-LTKSNKKCLSQFLIQEG 495
            L G+V++Y R+VKTG WH +RE+EPKD++ +T + R+L  STY  L    G   D T   TY   C   Q K ++ E  K   M + E + S + D +           FPRH   +D+  +E+TY  DY      + I        ++E  D D LT +NK+ LSQF    G
Sbjct:   10 LSGNVANYGRIVKTGKWHLSREAEPKDYEISTDKVRNLKRSTYDRLAAESGAFEDST---TYGSMCDQLQTKGDYTEHPKA-SMVTAETVASVVLDRDIGRPSRGPGALFPRHSSEYDKHHLESTYNADYGDRVTDEQIKQKCESENKVERPDEDLLTAANKRPLSQFCDTAG 178

HSP 2 Score: 43.1282 bits (100), Expect = 1.884e-20
Identity = 17/30 (56.67%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  477 ISDPGGYNRPGHNTWWDESGIYANSHLHRQ 566
              D  G+ R G NTW DE+G+YAN+HL RQ
Sbjct:  173 FCDTAGHRRQGRNTWQDETGVYANTHLRRQ 202
[Close]
BLAST of SMED30010410 vs. TrEMBL
Match: A0A3S1BJS3 (Uncharacterized protein OS=Elysia chlorotica OX=188477 GN=EGW08_009816 PE=4 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 5.801e-19
Identity = 59/169 (34.91%), Postives = 88/169 (52.07%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYLGGLPDKT-PLSTYQEFCQQ--------KKINHLELDKGMPMFSVEA-IDSPLFDANYNDDG-FPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIET----------KDPDL-TKSNKKCLSQF 480
            LR D  +YSR +   +WHQAR++EPKD+D N A  R++  STY  +G + D + P STYQE   Q         + NH  L +   + ++ A ID  +     + +   P H+  +++  ++TTY  DYV PYP   ++ E           KD +L TK+ KK  SQF
Sbjct:   23 LRSDHMNYSRAILNSHWHQARQAEPKDYDLNKAPLRNMTQSTYNRIGNVTDGSLPDSTYQEQANQVFLKPMYEDRDNHKSLVQMETLGTINAGIDRKIPGQECSSNSVLPCHKKDYNKFYLDTTYVSDYVAPYPLSKVDTEAQRQADMEQLKKDGELKTKAYKKMKSQF 191

HSP 2 Score: 37.3502 bits (85), Expect = 5.801e-19
Identity = 17/35 (48.57%), Postives = 20/35 (57.14%), Query Frame = 3 
Query:  462 KVFISISDPGGYNRPGHNTWWDESGIYANSHLHRQ 566
            K+    +D   Y R G NTW DESG+Y NSH   Q
Sbjct:  186 KMKSQFTDTADYRRNGWNTWQDESGVYTNSHYKAQ 220
[Close]
BLAST of SMED30010410 vs. TrEMBL
Match: A0A2T7NQI7 (Uncharacterized protein OS=Pomacea canaliculata OX=400727 GN=C0Q70_16706 PE=4 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 5.032e-18
Identity = 64/187 (34.22%), Postives = 90/187 (48.13%), Query Frame = 1 
Query:   37 ALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYLGGLPDKT-PLSTYQEFCQQKKINHL--ELDKGMPMFSVEAIDSPLFDANYNDDGF------PRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSN---KKCLSQFLIQEGITVQDITRGGTNREYMQILIYI 561
            +LR D   YSR V T  WHQARE+EPKD D N   +R+LH STY  +  + D + P +TYQE  +Q ++  L  E D    +F+VE +     D +  D  +      P H     +   ETTY  DY+  +P++   +  K P+   S+   +KC SQF         D  R G N    +  IY 
Sbjct:   17 SLRSDHMDYSRGVFTSKWHQAREAEPKDHDLNHEPKRNLHRSTYNRMATVTDGSLPKTTYQEHSRQIELKPLFDEKDNRRSLFNVETVRVTDIDRDTGDPKYGYGSVLPHHHPDHFKFHSETTYNADYIVHFPYESAPVPEKSPEYEVSSPAYRKCHSQF-----TDTADYRRPGRNTWMDESGIYT 198
[Close]
BLAST of SMED30010410 vs. Ensembl Cavefish
Match: C9orf135 (chromosome unknown C9orf135 homolog [Source:NCBI gene;Acc:103044504])

HSP 1 Score: 50.0618 bits (118), Expect = 2.480e-7
Identity = 36/120 (30.00%), Postives = 56/120 (46.67%), Query Frame = 1 
Query:   61 YSRVVKTGNWHQARESEPKDFDFNTAE--QRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAIDSPLFDANYN---DDGFPRHRFGFDRQQMETTYAVDYVYPY 405
            YSR     NWH+ RE+EPKD+D +     Q+ LH STYK+ G   +    +T +    Q  +      + +P   V+A      +++         PRH   +    + TTYA+DY  P+
Sbjct:    3 YSRPKLIYNWHKYREAEPKDYDISACPEGQKKLHKSTYKHFGTYINTEWTTTAEAQMSQYHLKKDYETREVPKSMVQASSFHTLESDRETGCKSVLPRHPPHYYEMDLCTTYALDYPPPH 122
[Close]
BLAST of SMED30010410 vs. Ensembl Nematostella
Match: EDO38748 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7SBZ6])

HSP 1 Score: 87.4261 bits (215), Expect = 4.564e-21
Identity = 64/195 (32.82%), Postives = 95/195 (48.72%), Query Frame = 1 
Query:   40 LRGDVSHYSRVVKTGNWHQARESEPKDFDFNT--AEQRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAID-SPLFDANYNDD------GF----PRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTI 585
            LR D   YSR     NWHQARE+EPK +D      E+R+LH+STYK +G + D + L       Q ++   L+ D        + +D S  ++ N+N D      GF    PRH    D++ ++TTY VDY  P+ ++  +    +PD++   +KC SQF       V D  R G N  + +  +Y    V  T 
Sbjct:   21 LRSDHMDYSRPALVSNWHQAREAEPKTYDIKDYLPEKRNLHNSTYKRIGDVTDGS-LPKTSSHDQMEQAVKLKSDFQTRDTRRQMVDMSSYYNTNFNRDTGAPDRGFGSVLPRHPPNHDKRYLDTTYKVDYTSPHHYEPAKTPPPEPDMSPLWRKCHSQF-----TDVDDYRRHGNNTWHEESGLYANSEVKRTF 209
[Close]
BLAST of SMED30010410 vs. Planmine SMEST
Match: SMESG000021757.1 (SMESG000021757.1)

HSP 1 Score: 328.561 bits (841), Expect = 2.831e-115
Identity = 159/180 (88.33%), Postives = 162/180 (90.00%), Query Frame = 1 
Query:   19 MNCPNGALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAIDSPLFDANYNDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKCLSQFLIQEGITVQDITRGGTNREYMQILIY 558
            MNCPNGALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAIDSPLFDANYNDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQH+EIETKDPDLTKSNKKCLSQF    G       R G N  + +  IY
Sbjct:    1 MNCPNGALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTYKYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAIDSPLFDANYNDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQHVEIETKDPDLTKSNKKCLSQFSDPGG-----YNRPGHNTWWDESGIY 175

HSP 2 Score: 100.138 bits (248), Expect = 1.023e-25
Identity = 44/49 (89.80%), Postives = 44/49 (89.80%), Query Frame = 3 
Query:  462 KVFISISDPGGYNRPGHNTWWDESGIYANSHLHRQKCLTDNLYGPFAQE 608
            K     SDPGGYNRPGHNTWWDESGIYANSHLHRQKCLTDNLYGPFAQE
Sbjct:  149 KCLSQFSDPGGYNRPGHNTWWDESGIYANSHLHRQKCLTDNLYGPFAQE 197
The following BLAST results are available for this feature:
BLAST of SMED30010410 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C9orf1355.677e-1028.43chromosome 9 open reading frame 135 [Source:HGNC S... [more]
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BLAST of SMED30010410 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 1
Match NameE-valueIdentityDescription
smim141.207e-828.50small integral membrane protein 14 [Source:Xenbase... [more]
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BLAST of SMED30010410 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 2
Match NameE-valueIdentityDescription
sp|Q32L77|CI135_BOVIN1.008e-1029.74Protein C9orf135 homolog OS=Bos taurus OX=9913 PE=... [more]
sp|Q5VTT2|CI135_HUMAN2.726e-928.43Protein C9orf135 OS=Homo sapiens OX=9606 GN=C9orf1... [more]
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BLAST of SMED30010410 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A1I8H0611.389e-2035.26Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A267DCI91.679e-2035.26Uncharacterized protein (Fragment) OS=Macrostomum ... [more]
A0A267GMR91.884e-2035.26Uncharacterized protein OS=Macrostomum lignano OX=... [more]
A0A3S1BJS35.801e-1934.91Uncharacterized protein OS=Elysia chlorotica OX=18... [more]
A0A2T7NQI75.032e-1834.22Uncharacterized protein OS=Pomacea canaliculata OX... [more]
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BLAST of SMED30010410 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
C9orf1352.480e-730.00chromosome unknown C9orf135 homolog [Source:NCBI g... [more]
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BLAST of SMED30010410 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO387484.564e-2132.82Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of SMED30010410 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010410 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000021757.12.831e-11588.33SMESG000021757.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010410 ID=SMED30010410|Name=SMED30010410|organism=Schmidtea mediterranea sexual|type=transcript|length=732bp
TCCGATCTGAGATTTTAAATGAATTGTCCCAATGGAGCTTTAAGAGGTGA
TGTTAGTCATTACAGTAGGGTTGTAAAAACTGGAAACTGGCATCAAGCTA
GGGAATCAGAACCTAAAGACTTTGATTTTAATACCGCTGAACAGAGATCA
CTTCATTCATCAACATATAAATATTTGGGAGGATTGCCTGACAAAACACC
ATTAAGCACTTATCAAGAATTTTGTCAACAAAAGAAAATTAATCATTTGG
AACTAGATAAAGGAATGCCAATGTTTTCTGTTGAAGCCATTGACAGTCCC
CTTTTTGATGCAAATTATAATGATGATGGATTTCCAAGACATCGATTTGG
GTTTGACAGGCAACAAATGGAAACAACCTATGCAGTCGATTATGTTTATC
CATATCCTTTTCAACATATCGAAATTGAAACAAAAGATCCAGATCTGACT
AAATCGAATAAAAAGTGTTTATCTCAATTTCTGATCCAGGAGGGTATAAC
CGTCCAGGACATAACACGTGGTGGGACGAATCGGGAATATATGCAAATTC
TCATTTACATCGCCAGAAATGTCTTACTGACAATCTATACGGGCCTTTTG
CTCAAGAGTGAACTAAAATCAGTCTTGTTATAATTATTTCATAAATGGAC
ATAAAAATCGTTTTAAATTTTCCAGTAATAATTTATAAAATAGCAAGTAA
CTCATGTTTAAATTGAAACATTAATGTTAGTG
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protein sequence of SMED30010410-orf-1

>SMED30010410-orf-1 ID=SMED30010410-orf-1|Name=SMED30010410-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=205bp
MNCPNGALRGDVSHYSRVVKTGNWHQARESEPKDFDFNTAEQRSLHSSTY
KYLGGLPDKTPLSTYQEFCQQKKINHLELDKGMPMFSVEAIDSPLFDANY
NDDGFPRHRFGFDRQQMETTYAVDYVYPYPFQHIEIETKDPDLTKSNKKC
LSQFLIQEGITVQDITRGGTNREYMQILIYIARNVLLTIYTGLLLKSELK
SVLL*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000016pharynx
PLANA:0000034epidermis
PLANA:0000099neuron
PLANA:0000418head
PLANA:0002089reproductive organ
Vocabulary: INTERPRO
TermDefinition
IPR027905DUF4572