SMED30010404

Overview
NameSMED30010404
Smed IDSMED30010404
Length (bp)4088
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of SMED30010404 (SMED30010404) t-SNE clustered cells

Violin plots show distribution of expression levels for SMED30010404 (SMED30010404) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of SMED30010404 (SMED30010404) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for SMED30010404 (SMED30010404) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
Sort Descending | Sort Ascending | Only Non-Zero Values | Tile/Chart | Reset

Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010404

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 11

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
epidermisSMED30010404h1SMcG0008281 dd_Smed_v4_5958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010404h1SMcG0008281 dd_Smed_v4_5958_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010404h1SMcG0008281 Contig33159newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010404h1SMcG0008281 Contig33159uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010404h1SMcG0008281 Contig36977newmark_estsPMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
Smed sexual biotypeSMED30010404h1SMcG0008281 Contig36977uc_Smed_v2PMID:29674431
Fincher et al., 2018		FACS sorted cell population adult hermaphrodite single-cell RNA-sequencing evidence
epidermisSMED30010404h1SMcG0008281 dd_Smed_v4_5391_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
cephalic gangliaSMED30010404h1SMcG0008281 dd_Smed_v4_5391_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30010404h1SMcG0008281 dd_Smed_v6_5391_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30010404h1SMcG0008281 dd_Smed_v6_5958_0dd_Smed_v6PMID:29674432
Plass et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Category 3 cellSMED30010404h1SMcG0008281 dd_Smed_v4_5958_0_1dd_Smed_v4PMID:28292427
Wurtzel et al., 2017		whole organism asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Alignments
SMED30010404 aligns in the following genomic locations:
Alignment LocationAlignment Score
v31.014920:13292..17379 -20287
Homology
[Close]
BLAST of SMED30010404 vs. Planmine SMEST
Match: SMESG000076927.1 (SMESG000076927.1)

HSP 1 Score: 2446.77 bits (6340), Expect = 0.000e+0
Identity = 1306/1315 (99.32%), Postives = 1313/1315 (99.85%), Query Frame = 1 
Query:   10 MLCLLGSKKFWLILIVYLTHFVQAQKNGNIEQVEDIVCESRISALNFKCNLEYGKIDLKAKVQIKTYKRLCDEVMKKPLEKCSNPLAENCNLATENMRMFCKNKYSDIKHFRIHARNIIKDVCQKAFNETKASCENSRLKLVKPKLGIIDKYLKLVNASKPKISTVKPKVQNINVDSVIGXXXXXXXXXXXXXXVNLFLNNILSQANYAPSKPTSPPRTIEKYRSKIQFLEKPIPKPVRWKPIKQNXXXXXXXXXXXXXXXXXXXXXXAQSIIDYVFGDYQNTIKTEDLINYVNDIYPDVDFNPKKPVRVSNNKPIVMPNNQYIINYIFGNNPSKIHRHFMVSFIERIMDTKYESGLILDRLSPIQSTFKPKHRFEKPYQPIHHLNRPYRLENDPIFQKPQHRFDRPYKSKRVPERSRSKYEIKKPVKYGREQKPANKIPHSIQLVSNYIFGESLNNVNKEDLVEYINEIYSDVELNDLKPSTGSHIDVKVPSIQSIIKFIFGKNPKKIHKHILISMIERILLIKEMPSKLIREKLASKLKPTFQNYPIAKRPSVSIQSIIDFIFGNNQKTIDQSFLIEFIKEIFADTKFDPKKPVKESNKPHIEMPKNQEIIEFIFGNKSIDIKKPVLIDFIDKIINNEQYHSEVLTFGKSDEKQKPTSRPNKLQKPIHLSKLKLGAEENNRNLINYVFGEAQYEIDNSLLREFLDRMFNKPNVDRPANIPISKPKSVANISSIPHPELVINYIFGNSKNKINKNDLDDFVNQAFGNVLPSKPIKDHRKEDDFYPKLQELIEYLFNRLKKNPQLFEDVSFGNKSYLLPVVEYILENYKHKINRHSYLNRNDPKFTKIDSKPNLLELITNVFGDSEPSKPVKNENLEKPIYHLYTQKPSRDESKENIGNRPTENDKKPFDISFVFNIISRLLGEKVETDRAENLDKGKISSPEHGRQHFPPLIQNGLVKIKPEQWKDLPEKHQMLLSILMRIIDALLKNHSQGLPGKVSDQDTVNDILDSFPFNSRKLEKISDKDKKLVISLVFKVIKEIFNQLSKHPKSSINAILSDLTETIGSARQESGRIRQQDIFKRPTVSFKPEREKPRKFDTSFVFKIIDELLNPGSIDSAANSRDESRNNIISIYDLFKDKNKAKEDEQSGQKAEEGEVHLRPAFIMLRDDERLDKETNAEQNDSGEKTTKPRKVSLKPKFKLVEDGNRNEEIDQETGSETQISKPSVEELQLPTKKPQSHAIENNKIQRESXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPMXXXXXXXXXXXXXXXXXXPIAQPKLA 3954
            MLCLLGSKKFWLILIVYLTHFVQAQKNGNIEQVEDIVCESRISALNFKCNLEYGKIDLKAKVQIKTYKRLCDEVMKKPLEKCSNPLA+NCNLATENMRMFCKNKYSDIKHFRIHARNIIKDVCQKAFNETKASCENSRLKLVKPKLGIIDKYLKLVNASKPKISTVKPKVQNINVDSVIGSSFSSN+NETSETEVNLFLNNILSQANYAPSKPTSPPRTIEKYRSKIQFLEKPIPKPVRWKPIKQNRFKPIPIRTKPIRPRPISISHRAQSIIDYVFGDYQNTIKTEDLINYVNDIY D DFNPKKPVRVSNNKPIVMPNNQYIINYIFGNNPSKIHRHFMVSFIERIMDTKYESGLILDRLSPIQSTFKPKHRFEKPYQPIHHLNRPYRLENDPIFQKPQHRFDRPYKSKRVPERSRSKYEIKKPVKYGREQKPANKIPHSIQLVSNYIFGESLNNVNKEDLVEYINEIYSDVELNDLKPSTGSHIDVKVPSIQSIIKFIFGKNPKKIHKHILISMIERILLIKEMPSKLIREKLASKLKPTFQNYPIAKRPSVSIQSIIDFIFGNNQKTIDQSFLIEFIKEIFADTKFDPKKPVKESNKPHIEMPKNQEIIEFIFGNKSIDIKKPVLIDFIDKIINNEQYHSEVLTFGKSDEKQKPTSRPNKL+KPIHLSKLKLGAEENNRNLINYVFGEAQYEIDNSLLREFLDRMFNKPNVDRPANIPISKPKSVANISSIPHPEL+INYIFGNSKNKINKNDLDDFVNQAFGNVLPSKPIKDHRKEDDFYPKLQELIEYLFNRLK+NPQLFEDVSFGNKSYLLPVVEYILENYKHKINRHSYLNRNDPKFTKIDSKPNLLELITNVFGDSEPSKPVKNENLEKPIYHLYTQKPSRDESKENIGNRPTENDKKPFDISFVFNIISRLLGEKVETDRAENLDKGKISSPEHGRQHFPPLIQNGLVKIKPEQWKDLPEKHQMLLSILMRIIDALLKNHSQGLPGKVSDQDTVNDILDSFPFNSRKLEKISDKDKKLVISLVFKVIKEIFNQLSKHPKSSINAILSDLTETIGSARQESGRIRQQDIFKRPTVSFKPEREKPRKFDTSFVFKIIDELLNPGSIDSAANSRDESRNNIISIYDLFKDKNKAKEDEQSGQKAEEGEVHLRPAFIMLRDDERLDKETNAEQNDSGEKTTKPRKVSLKPKFKLVEDGNRNEEIDQETGSETQISKPSVEELQ+PTKKPQSHAIENNKIQRESTKKPKA+TKKMKKANNKSKIINIKTTKKIKKNRKSTIKPMKANKAAIKTKKTTKIIKKPIAQPKLA
Sbjct:    1 MLCLLGSKKFWLILIVYLTHFVQAQKNGNIEQVEDIVCESRISALNFKCNLEYGKIDLKAKVQIKTYKRLCDEVMKKPLEKCSNPLAKNCNLATENMRMFCKNKYSDIKHFRIHARNIIKDVCQKAFNETKASCENSRLKLVKPKLGIIDKYLKLVNASKPKISTVKPKVQNINVDSVIGSSFSSNDNETSETEVNLFLNNILSQANYAPSKPTSPPRTIEKYRSKIQFLEKPIPKPVRWKPIKQNRFKPIPIRTKPIRPRPISISHRAQSIIDYVFGDYQNTIKTEDLINYVNDIYADGDFNPKKPVRVSNNKPIVMPNNQYIINYIFGNNPSKIHRHFMVSFIERIMDTKYESGLILDRLSPIQSTFKPKHRFEKPYQPIHHLNRPYRLENDPIFQKPQHRFDRPYKSKRVPERSRSKYEIKKPVKYGREQKPANKIPHSIQLVSNYIFGESLNNVNKEDLVEYINEIYSDVELNDLKPSTGSHIDVKVPSIQSIIKFIFGKNPKKIHKHILISMIERILLIKEMPSKLIREKLASKLKPTFQNYPIAKRPSVSIQSIIDFIFGNNQKTIDQSFLIEFIKEIFADTKFDPKKPVKESNKPHIEMPKNQEIIEFIFGNKSIDIKKPVLIDFIDKIINNEQYHSEVLTFGKSDEKQKPTSRPNKLRKPIHLSKLKLGAEENNRNLINYVFGEAQYEIDNSLLREFLDRMFNKPNVDRPANIPISKPKSVANISSIPHPELIINYIFGNSKNKINKNDLDDFVNQAFGNVLPSKPIKDHRKEDDFYPKLQELIEYLFNRLKQNPQLFEDVSFGNKSYLLPVVEYILENYKHKINRHSYLNRNDPKFTKIDSKPNLLELITNVFGDSEPSKPVKNENLEKPIYHLYTQKPSRDESKENIGNRPTENDKKPFDISFVFNIISRLLGEKVETDRAENLDKGKISSPEHGRQHFPPLIQNGLVKIKPEQWKDLPEKHQMLLSILMRIIDALLKNHSQGLPGKVSDQDTVNDILDSFPFNSRKLEKISDKDKKLVISLVFKVIKEIFNQLSKHPKSSINAILSDLTETIGSARQESGRIRQQDIFKRPTVSFKPEREKPRKFDTSFVFKIIDELLNPGSIDSAANSRDESRNNIISIYDLFKDKNKAKEDEQSGQKAEEGEVHLRPAFIMLRDDERLDKETNAEQNDSGEKTTKPRKVSLKPKFKLVEDGNRNEEIDQETGSETQISKPSVEELQIPTKKPQSHAIENNKIQRESTKKPKAITKKMKKANNKSKIINIKTTKKIKKNRKSTIKPMKANKAAIKTKKTTKIIKKPIAQPKLA 1315
[Close]
BLAST of SMED30010404 vs. Planmine SMEST
Match: SMESG000018472.1 (SMESG000018472.1)

HSP 1 Score: 66.6254 bits (161), Expect = 1.016e-10
Identity = 41/154 (26.62%), Postives = 70/154 (45.45%), Query Frame = 1 
Query:   10 MLCLLGSKKFWLILIVYLTHFVQAQKNGNI-------------EQVEDIVCESRISALNFKCNLEYGKIDLKAKVQIKTYKRLCDEVMKKPLEKCSNPLAENCNLATENMRMFCKNKYSDIKHFRIHARNIIKDVCQKAFNETKASCENSRLKL 432
            M+C       +   I++     Q  KNGN+             EQ+E I+C+ R ++  FKCN EY KIDLK+ ++    + +C +    P ++C       CN     ++ +C  + ++I+  R+   NII   C K        C+N + K+
Sbjct:    1 MMC-----NIFCFFILFTVANCQGFKNGNVQKIANNGKTVYTLEQIELIICKHREASYLFKCNFEYAKIDLKSDLEENKRQAICKDKSLDPNKQCQALRNVGCN-PYNGLKSWCIKRKAEIEEQRLLWHNIIAVTCHKNLVRHTEICKNGKNKM 148
The following BLAST results are available for this feature:
BLAST of SMED30010404 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of SMED30010404 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 2
Match NameE-valueIdentityDescription
SMESG000076927.10.000e+099.32SMESG000076927.1[more]
SMESG000018472.11.016e-1026.62SMESG000018472.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010404 ID=SMED30010404|Name=SMED30010404|organism=Schmidtea mediterranea sexual|type=transcript|length=4088bp
ATTTTTACAATGTTATGTTTGTTAGGATCAAAAAAATTTTGGTTGATCTT
GATTGTCTATTTGACGCATTTTGTTCAAGCTCAAAAAAATGGCAACATCG
AACAAGTCGAAGACATTGTCTGTGAATCTCGAATCTCTGCTCTCAATTTC
AAATGCAATCTTGAATATGGAAAAATTGACTTGAAAGCCAAAGTTCAAAT
CAAAACATACAAAAGGCTTTGTGATGAGGTCATGAAAAAACCTCTAGAGA
AATGCAGCAATCCTTTGGCGGAGAATTGCAATTTGGCCACTGAAAATATG
AGAATGTTTTGCAAGAACAAATATTCCGATATCAAACATTTCCGAATCCA
TGCTCGTAATATCATCAAAGATGTTTGCCAGAAAGCGTTCAACGAAACGA
AAGCATCATGCGAGAACAGTAGGTTGAAGTTAGTTAAGCCAAAACTCGGA
ATCATTGATAAATATTTGAAGTTAGTAAATGCATCAAAACCGAAAATTTC
AACCGTGAAACCAAAAGTGCAAAATATAAACGTTGATTCTGTGATAGGTT
CTTCATTCAGTTCAAATGAAAATGAAACATCCGAAACCGAAGTCAACTTG
TTTCTCAATAATATCTTATCACAAGCCAATTATGCTCCAAGTAAACCAAC
AAGTCCGCCAAGAACCATTGAAAAATACAGGTCGAAAATTCAATTCCTTG
AAAAGCCAATACCAAAACCAGTTAGATGGAAGCCGATCAAGCAAAACCGA
TTCAAACCAATTCCGATCAGAACTAAACCGATCAGGCCAAGACCAATCTC
GATTTCACATCGAGCACAATCAATAATCGATTACGTATTCGGTGATTATC
AAAATACAATCAAAACCGAAGATTTAATAAATTACGTCAATGACATTTAT
CCTGATGTAGATTTCAATCCCAAAAAGCCAGTAAGGGTGTCAAATAATAA
ACCTATTGTTATGCCAAATAATCAATATATAATTAATTATATATTCGGAA
ACAATCCAAGCAAAATTCATAGACATTTTATGGTTTCATTCATTGAACGT
ATCATGGATACCAAATACGAATCAGGACTAATTCTAGACCGATTAAGTCC
GATTCAGTCAACATTCAAACCAAAACATAGATTTGAAAAGCCTTATCAAC
CAATTCACCATTTAAATCGGCCATATAGATTAGAAAATGATCCTATATTT
CAAAAACCTCAACACCGTTTTGACCGACCATACAAGTCGAAAAGAGTTCC
TGAGAGGTCAAGATCGAAATATGAGATTAAAAAACCAGTTAAATATGGAA
GAGAGCAAAAACCAGCAAACAAAATTCCTCATAGCATACAATTAGTAAGT
AATTATATCTTTGGAGAGTCTCTAAACAATGTAAACAAAGAAGATTTGGT
GGAATACATAAATGAAATATATTCCGATGTTGAATTAAATGATTTGAAAC
CCTCCACAGGTTCACATATAGATGTAAAAGTTCCAAGCATTCAATCGATA
ATAAAATTCATTTTTGGAAAAAATCCAAAGAAAATCCATAAACACATTTT
GATATCAATGATCGAAAGAATTTTGCTAATCAAAGAAATGCCATCGAAAT
TAATTAGAGAAAAGCTTGCGAGTAAGTTAAAGCCGACATTCCAAAATTAT
CCAATAGCAAAGAGACCGTCTGTCAGCATCCAATCAATTATTGATTTCAT
TTTTGGAAATAATCAGAAAACTATCGACCAATCATTTTTGATTGAATTTA
TCAAAGAAATTTTTGCTGACACTAAATTCGATCCGAAAAAACCGGTAAAA
GAATCCAATAAACCTCATATTGAAATGCCAAAAAATCAGGAAATCATCGA
ATTTATATTTGGAAACAAATCGATCGATATAAAAAAACCAGTTTTAATAG
ACTTTATCGACAAGATAATCAACAATGAGCAATATCATTCGGAAGTGTTG
ACATTCGGTAAATCGGACGAGAAGCAAAAACCAACTTCAAGGCCAAATAA
ATTACAAAAACCAATTCATTTATCAAAGTTGAAACTTGGTGCCGAAGAAA
ACAACAGGAATCTGATCAATTATGTTTTTGGAGAAGCTCAATATGAAATA
GACAACTCTTTGTTGCGAGAGTTCTTGGATCGAATGTTTAATAAACCGAA
TGTAGACCGTCCGGCTAATATACCAATATCTAAGCCAAAATCAGTAGCAA
ATATTAGCAGTATACCCCATCCGGAACTTGTTATCAACTATATTTTCGGG
AACAGTAAAAATAAAATTAATAAAAACGATTTAGACGATTTTGTGAACCA
AGCCTTTGGAAATGTGCTACCATCAAAACCAATTAAAGACCACCGAAAAG
AGGATGATTTCTATCCGAAGTTGCAAGAACTAATCGAATATTTATTTAAC
CGATTAAAAAAAAACCCACAATTGTTTGAAGATGTGTCCTTTGGGAATAA
ATCCTACTTACTTCCTGTTGTAGAATATATTCTAGAAAATTACAAGCACA
AAATTAATAGACATTCCTATTTGAATCGAAACGATCCGAAATTCACCAAG
ATTGATTCTAAGCCGAACTTGTTGGAATTGATAACAAATGTCTTCGGAGA
TTCTGAACCATCAAAACCAGTGAAAAATGAAAATTTAGAGAAACCGATTT
ACCATTTGTACACTCAAAAACCGAGTCGAGATGAATCAAAAGAAAATATT
GGAAATAGACCAACTGAAAACGATAAAAAACCATTTGACATTTCTTTTGT
TTTCAATATTATTAGTAGACTTCTTGGGGAAAAAGTAGAAACAGATAGAG
CTGAGAATTTGGACAAGGGTAAAATTAGTAGCCCAGAGCATGGAAGACAG
CATTTTCCGCCCTTGATTCAAAATGGATTAGTGAAAATCAAACCAGAACA
ATGGAAAGATCTTCCAGAAAAGCATCAAATGCTTTTGTCTATTTTGATGA
GAATAATTGATGCATTGTTGAAAAACCATTCTCAGGGTTTGCCAGGAAAA
GTCAGTGATCAAGATACAGTAAATGATATTCTTGATAGCTTTCCATTCAA
CTCACGAAAACTGGAAAAAATATCCGATAAAGATAAAAAATTAGTAATTT
CTTTGGTATTTAAAGTAATTAAAGAAATTTTCAATCAACTATCAAAACAT
CCAAAGTCAAGTATAAATGCGATTTTATCAGATTTGACTGAAACAATTGG
TAGTGCACGTCAAGAATCAGGGAGGATACGTCAGCAAGACATTTTTAAAA
GACCAACAGTTTCGTTCAAGCCTGAACGAGAAAAGCCTAGAAAATTCGAT
ACGTCTTTTGTTTTTAAAATCATAGATGAGTTGCTGAATCCCGGTTCAAT
TGATTCAGCTGCAAATAGTCGGGATGAATCTCGAAATAACATTATTTCAA
TTTATGATTTGTTTAAAGACAAAAACAAAGCAAAGGAAGATGAACAAAGT
GGACAGAAAGCCGAAGAGGGAGAAGTACATTTGCGGCCTGCGTTCATCAT
GCTAAGAGATGATGAAAGATTAGATAAAGAAACTAACGCCGAACAAAATG
ACAGTGGAGAAAAAACTACAAAACCAAGAAAAGTTTCATTGAAACCTAAA
TTTAAATTAGTTGAAGATGGAAATAGAAATGAAGAAATAGATCAAGAAAC
GGGTTCAGAAACTCAAATTAGTAAACCTTCTGTAGAAGAACTTCAATTAC
CAACAAAGAAGCCTCAATCACATGCCATTGAAAATAACAAAATTCAAAGA
GAAAGTACTAAAAAGCCAAAAGCAGTTACCAAGAAAATGAAAAAAGCAAA
CAATAAATCTAAAATAATCAACATAAAAACAACAAAGAAAATTAAAAAAA
ATAGAAAATCGACGATTAAACCAATGAAAGCCAATAAAGCTGCAATAAAA
ACAAAGAAAACAACTAAAATAATCAAAAAACCGATTGCTCAACCTAAATT
GGCAACCCGTAAATTGTTTAAAACGACAAAGAAACCGAAGAAGGTAATTA
AGAAATCTAAAATGACTACTAAAAATTTAAATAAAACGTCAAAAAAACCG
AAGAAGACAAGTGAGAAATCGAAAAAGTCTACTATAAT
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protein sequence of SMED30010404-orf-1

>SMED30010404-orf-1 ID=SMED30010404-orf-1|Name=SMED30010404-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=1362bp
IFTMLCLLGSKKFWLILIVYLTHFVQAQKNGNIEQVEDIVCESRISALNF
KCNLEYGKIDLKAKVQIKTYKRLCDEVMKKPLEKCSNPLAENCNLATENM
RMFCKNKYSDIKHFRIHARNIIKDVCQKAFNETKASCENSRLKLVKPKLG
IIDKYLKLVNASKPKISTVKPKVQNINVDSVIGSSFSSNENETSETEVNL
FLNNILSQANYAPSKPTSPPRTIEKYRSKIQFLEKPIPKPVRWKPIKQNR
FKPIPIRTKPIRPRPISISHRAQSIIDYVFGDYQNTIKTEDLINYVNDIY
PDVDFNPKKPVRVSNNKPIVMPNNQYIINYIFGNNPSKIHRHFMVSFIER
IMDTKYESGLILDRLSPIQSTFKPKHRFEKPYQPIHHLNRPYRLENDPIF
QKPQHRFDRPYKSKRVPERSRSKYEIKKPVKYGREQKPANKIPHSIQLVS
NYIFGESLNNVNKEDLVEYINEIYSDVELNDLKPSTGSHIDVKVPSIQSI
IKFIFGKNPKKIHKHILISMIERILLIKEMPSKLIREKLASKLKPTFQNY
PIAKRPSVSIQSIIDFIFGNNQKTIDQSFLIEFIKEIFADTKFDPKKPVK
ESNKPHIEMPKNQEIIEFIFGNKSIDIKKPVLIDFIDKIINNEQYHSEVL
TFGKSDEKQKPTSRPNKLQKPIHLSKLKLGAEENNRNLINYVFGEAQYEI
DNSLLREFLDRMFNKPNVDRPANIPISKPKSVANISSIPHPELVINYIFG
NSKNKINKNDLDDFVNQAFGNVLPSKPIKDHRKEDDFYPKLQELIEYLFN
RLKKNPQLFEDVSFGNKSYLLPVVEYILENYKHKINRHSYLNRNDPKFTK
IDSKPNLLELITNVFGDSEPSKPVKNENLEKPIYHLYTQKPSRDESKENI
GNRPTENDKKPFDISFVFNIISRLLGEKVETDRAENLDKGKISSPEHGRQ
HFPPLIQNGLVKIKPEQWKDLPEKHQMLLSILMRIIDALLKNHSQGLPGK
VSDQDTVNDILDSFPFNSRKLEKISDKDKKLVISLVFKVIKEIFNQLSKH
PKSSINAILSDLTETIGSARQESGRIRQQDIFKRPTVSFKPEREKPRKFD
TSFVFKIIDELLNPGSIDSAANSRDESRNNIISIYDLFKDKNKAKEDEQS
GQKAEEGEVHLRPAFIMLRDDERLDKETNAEQNDSGEKTTKPRKVSLKPK
FKLVEDGNRNEEIDQETGSETQISKPSVEELQLPTKKPQSHAIENNKIQR
ESTKKPKAVTKKMKKANNKSKIINIKTTKKIKKNRKSTIKPMKANKAAIK
TKKTTKIIKKPIAQPKLATRKLFKTTKKPKKVIKKSKMTTKNLNKTSKKP
KKTSEKSKKSTI
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Aliases
The feature 'SMED30010404' has the following synonyms
Synonym
pmp-5
Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000035Category 3 cell
PLANA:0002032epidermal cell
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1142..1218
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1321..1355
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1142..1243
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1318..1362
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 887..906
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1283..1305
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 886..906
NoneNo IPR availableSIGNALP_EUKSignalP-noTMSignalP-noTMcoord: 1..27
score: 0.86