SMED30010350
Overview
Neoblast Clusters
Zeng et. al., 2018▻ Overview▻ Neoblast Population▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Overview
Single cell RNA-seq of pluripotent neoblasts and its early progeniesWe isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)Explore this single cell expression dataset with our NB Cluster Shiny App
Neoblast Population
Sub-lethal Irradiated Surviving X1 and X2 Cell Population
Embryonic Expression
Davies et. al., 2017
Hover the mouse over a column in the graph to view average RPKM values per sample. Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult. back to top Anatomical Expression
PAGE et. al., 2020SMED30010350 has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGEPAGE Curations: 27Note: Hover over icons to view figure legend
Alignments
SMED30010350 aligns in the following genomic locations:
Homology
BLAST of SMED30010350 vs. TrEMBL
Match: I1ZIB2 (Uncharacterized protein OS=Schmidtea mediterranea OX=79327 PE=2 SV=1) HSP 1 Score: 85.8853 bits (211), Expect = 1.224e-19 Identity = 38/40 (95.00%), Postives = 39/40 (97.50%), Query Frame = 2 Query: 140 MKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFCGL 259 MK+FQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGG CGL Sbjct: 1 MKLFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGLCGL 40
BLAST of SMED30010350 vs. Planmine SMEST
Match: SMESG000017801.1 (SMESG000017801.1) HSP 1 Score: 142.895 bits (359), Expect = 3.614e-45 Identity = 67/68 (98.53%), Postives = 67/68 (98.53%), Query Frame = 2 Query: 56 IKTEELANDKSLIKIKAVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFCGL 259 IKTEELANDKSLIKIKAVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGG CGL Sbjct: 21 IKTEELANDKSLIKIKAVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGLCGL 88
BLAST of SMED30010350 vs. Planmine SMEST
Match: SMESG000017804.1 (SMESG000017804.1) HSP 1 Score: 140.198 bits (352), Expect = 4.581e-44 Identity = 65/68 (95.59%), Postives = 66/68 (97.06%), Query Frame = 2 Query: 56 IKTEELANDKSLIKIKAVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFCGL 259 IKTEELANDKSLIKIKAVALEN NNRLQMK+FQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGG CGL Sbjct: 21 IKTEELANDKSLIKIKAVALENLNNRLQMKLFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGLCGL 88
BLAST of SMED30010350 vs. Planmine SMEST
Match: SMESG000017789.1 (SMESG000017789.1) HSP 1 Score: 44.669 bits (104), Expect = 1.760e-6 Identity = 19/50 (38.00%), Postives = 27/50 (54.00%), Query Frame = 2 Query: 104 AVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFC 253 +V N + L +K + + CP GY PC G+WCCP G C+ G+C Sbjct: 65 SVLARNIKSWLHIKHTNVRLKGYCPEGYFPCSHGLWCCPDGSLCMTDGWC 114
BLAST of SMED30010350 vs. Planmine SMEST
Match: SMESG000017789.1 (SMESG000017789.1) HSP 1 Score: 44.669 bits (104), Expect = 1.838e-6 Identity = 19/50 (38.00%), Postives = 27/50 (54.00%), Query Frame = 2 Query: 104 AVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFC 253 +V N + L +K + + CP GY PC G+WCCP G C+ G+C Sbjct: 59 SVLARNIKSWLHIKHTNVRLKGYCPEGYFPCSHGLWCCPDGSLCMTDGWC 108
BLAST of SMED30010350 vs. Planmine SMEST
Match: SMESG000017789.1 (SMESG000017789.1) HSP 1 Score: 44.669 bits (104), Expect = 2.491e-6 Identity = 19/50 (38.00%), Postives = 27/50 (54.00%), Query Frame = 2 Query: 104 AVALENANNRLQMKIFQMLMANSCPSGYHPCPDGVWCCPIGWNCLGGGFC 253 +V N + L +K + + CP GY PC G+WCCP G C+ G+C Sbjct: 84 SVLARNIKSWLHIKHTNVRLKGYCPEGYFPCSHGLWCCPDGSLCMTDGWC 133 The following BLAST results are available for this feature:
BLAST of SMED30010350 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99) Total hits: 0
BLAST of SMED30010350 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt) Total hits: 0
BLAST of SMED30010350 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL) Total hits: 1
BLAST of SMED30010350 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46) Total hits: 0
BLAST of SMED30010350 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99) Total hits: 0
BLAST of SMED30010350 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST) Total hits: 5
Analyses
This transcript is derived from or has results from the following analyses Sequences
The following sequences are available for this feature:
transcript sequence >SMED30010350 ID=SMED30010350|Name=SMED30010350|organism=Schmidtea mediterranea sexual|type=transcript|length=441bpback to top Annotated Terms
The following terms have been associated with this transcript:
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
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