28S ribosomal protein S14

Overview
Name28S ribosomal protein S14
Smed IDSMED30010291
Length (bp)793
Neoblast Clusters

Zeng et. al., 2018



▻ Overview

▻ Neoblast Population

▻ Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

 

Overview

 

Single cell RNA-seq of pluripotent neoblasts and its early progenies


We isolated X1 neoblasts cells enriched in high piwi-1 expression (Neoblast Population), and profiled ∼7,614 individual cells via scRNA-seq. Unsupervised analyses uncovered 12 distinct classes from 7,088 high-quality cells. We designated these classes Nb1 to Nb12 and ordered them based on high (Nb1) to low (Nb12) piwi-1 expression levels. We further defined groups of genes that best classified the cells parsed into 12 distinct cell clusters to generate a scaled expression heat map of discriminative gene sets for each cluster. Expression of each cluster’s gene signatures was validated using multiplex fluorescence in situ hybridization (FISH) co-stained with piwi-1 and largely confirmed the cell clusters revealed by scRNA-seq.

We also tested sub-lethal irradiation exposure. To profile rare pluripotent stem cells (PSCs) and avoid interference from immediate progenitor cells, we determined a time point after sub-lethal irradiation (7 DPI) with minimal piwi-1+ cells, followed by isolation and single-cell RNA-seq of 1,200 individual cells derived from X1 (Piwi-1 high) and X2 (Piwi-1 low) cell populations (Sub-lethal Irradiated Surviving X1 and X2 Cell Population)



Explore this single cell expression dataset with our NB Cluster Shiny App



 

Neoblast Population

 

t-SNE plot shows two-dimensional representation of global gene expression relationships among all neoblasts (n = 7,088 after filter). Cluster identity was assigned based on the top 10 marker genes of each cluster (Table S2), followed by inspection of RNA in situ hybridization patterns. Neoblast groups, Nb.


Expression of 28S ribosomal protein S14 (SMED30010291) t-SNE clustered cells

Violin plots show distribution of expression levels for 28S ribosomal protein S14 (SMED30010291) in cells (dots) of each of the 12 neoblast clusters.

 

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Sub-lethal Irradiated Surviving X1 and X2 Cell Population

 

t-SNE plot of surviving X1 and X2 cells (n = 1,039 after QC filter) after sub-lethal irradiation. Colors indicate unbiased cell classification via graph-based clustering. SL, sub-lethal irradiated cell groups.

Expression of 28S ribosomal protein S14 (SMED30010291) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells.

Violin plots show distribution of expression levels for 28S ribosomal protein S14 (SMED30010291) in cells (dots) of each of the 10 clusters of sub-leathally irradiated X1 and X2 cells.

 

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Embryonic Expression

Davies et. al., 2017

Hover the mouse over a column in the graph to view average RPKM values per sample.
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Embryonic Stages: Y: yolk. S2-S8: Stages 2-8. C4: asexual adult. SX: virgin, sexually mature adult.
For further information about sample preparation and analysis for the single animal RNA-Seq experiment, please refer to the Materials and Methods

 

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Anatomical Expression

PAGE et. al., 2020



SMED30010291

has been reported as being expressed in these anatomical structures and/or regions. Read more about PAGE



PAGE Curations: 4

  
Expressed InReference TranscriptGene ModelsPublished TranscriptTranscriptomePublicationSpecimenLifecycleEvidence
X1 cellSMED30010291h1SMcG0008516 SmedASXL_019139SmedAsxl_ww_GCZZ01PMID:26114597
Zhu et al., 2015		FACS sorted cell population asexual adult RNA-sequencing evidence
neoblastSMED30010291h1SMcG0008516 dd_Smed_v4_4035_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior sideSMED30010291h1SMcG0008516 dd_Smed_v4_4035_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
posterior muscle cellSMED30010291h1SMcG0008516 dd_Smed_v4_4035_0_1dd_Smed_v4PMID:29674431
Fincher et al., 2018		FACS sorted cell population asexual adult single-cell RNA-sequencing evidence
Note: Hover over icons to view figure legend
Homology
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Human
Match: MRPS14 (mitochondrial ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:14049])

HSP 1 Score: 78.5666 bits (192), Expect = 8.692e-18
Identity = 37/100 (37.00%), Postives = 65/100 (65.00%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            +++ R+ RD K+R +++ +   R R+ +L KN ++P+ ++ VA  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+   SG+ +  +
Sbjct:   29 YVDWRMWRDVKRRKMAYEYADERLRINSLRKNTILPKILQDVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGIQRATW 128
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Fly
Match: mRpS14 (gene:FBgn0044030 transcript:FBtr0074785)

HSP 1 Score: 72.4034 bits (176), Expect = 9.296e-16
Identity = 37/91 (40.66%), Postives = 57/91 (62.64%), Query Frame = 2 
Query:  293 RIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
            ++IRD K+R         R R+ +L KN ++P  +++VA +++   PRDS+  RV+  C +T R RG   ++ +SRI+WRH ADYN  SGV
Sbjct:   33 KMIRDVKRRKCVKENAVERLRVNSLRKNDILPPELREVADAEIAAFPRDSSLVRVRERCALTSRPRGVVHKYRLSRIVWRHLADYNKLSGV 123
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Fly
Match: mRpS14 (gene:FBgn0044030 transcript:FBtr0346015)

HSP 1 Score: 72.4034 bits (176), Expect = 9.296e-16
Identity = 37/91 (40.66%), Postives = 57/91 (62.64%), Query Frame = 2 
Query:  293 RIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
            ++IRD K+R         R R+ +L KN ++P  +++VA +++   PRDS+  RV+  C +T R RG   ++ +SRI+WRH ADYN  SGV
Sbjct:   33 KMIRDVKRRKCVKENAVERLRVNSLRKNDILPPELREVADAEIAAFPRDSSLVRVRERCALTSRPRGVVHKYRLSRIVWRHLADYNKLSGV 123
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Zebrafish
Match: mrps14 (mitochondrial ribosomal protein S14 [Source:ZFIN;Acc:ZDB-GENE-060825-75])

HSP 1 Score: 82.4185 bits (202), Expect = 3.191e-19
Identity = 38/113 (33.63%), Postives = 69/113 (61.06%), Query Frame = 2 
Query:  227 EKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
             ++ CR P          +++ R++RD K+R ++F +   R R+ A+ KN ++P+ ++++A  ++  LPRDS   R++  C++T R R  K RW +SRI++R  AD++  SGV
Sbjct:   21 SRQACRSPVAVMEQVRSYYVDWRMLRDVKRRQMAFEYADTRLRINAIRKNTILPKELQEIADKEIAALPRDSCPVRIRNRCVLTSRPRAVKRRWRLSRIVFRDLADHSQMSGV 133
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Xenopus
Match: garnl3 (GTPase activating Rap/RanGAP domain-like 3 [Source:Xenbase;Acc:XB-GENE-986411])

HSP 1 Score: 76.2554 bits (186), Expect = 4.808e-17
Identity = 36/98 (36.73%), Postives = 63/98 (64.29%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            +++ R+ RD K+R +++ +   R R+ AL KN ++P+ +++VA  ++   P DS   R++  C++T R RG K RW +SRI++RH AD N  SG+ + 
Sbjct:   20 YVDWRMFRDVKRRKMAYEYADERLRVNALRKNIILPKELREVADKEIAAFPVDSCPVRIRNRCVLTSRPRGVKRRWRLSRIVFRHLADNNQMSGIQRA 117
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Xenopus
Match: garnl3 (GTPase activating Rap/RanGAP domain-like 3 [Source:Xenbase;Acc:XB-GENE-986411])

HSP 1 Score: 75.8702 bits (185), Expect = 1.059e-16
Identity = 36/98 (36.73%), Postives = 63/98 (64.29%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            +++ R+ RD K+R +++ +   R R+ AL KN ++P+ +++VA  ++   P DS   R++  C++T R RG K RW +SRI++RH AD N  SG+ + 
Sbjct:   32 YVDWRMFRDVKRRKMAYEYADERLRVNALRKNIILPKELREVADKEIAAFPVDSCPVRIRNRCVLTSRPRGVKRRWRLSRIVFRHLADNNQMSGIQRA 129
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Mouse
Match: Mrps14 (mitochondrial ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:1928141])

HSP 1 Score: 87.8113 bits (216), Expect = 1.608e-21
Identity = 42/112 (37.50%), Postives = 71/112 (63.39%), Query Frame = 2 
Query:  230 KELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
            KE    PP         +++ R++RD K+R +++ +   R R+ +L KN ++P++++++A  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+ L SGV
Sbjct:    6 KEPSAVPPSASGQVRGYYVDWRMLRDLKRRKMAYEYADERLRINSLRKNTILPKDLQEMAGDEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGLLSGV 117
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Mouse
Match: Mrps14 (mitochondrial ribosomal protein S14 [Source:MGI Symbol;Acc:MGI:1928141])

HSP 1 Score: 85.8853 bits (211), Expect = 9.932e-21
Identity = 40/107 (37.38%), Postives = 69/107 (64.49%), Query Frame = 2 
Query:  245 FPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
             PP         +++ R++RD K+R +++ +   R R+ +L KN ++P++++++A  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+ L SGV
Sbjct:   17 VPPSASGQVRGYYVDWRMLRDLKRRKMAYEYADERLRINSLRKNTILPKDLQEMAGDEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGLLSGV 123
[Close]
BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|Q9CR88|RT14_MOUSE (28S ribosomal protein S14, mitochondrial OS=Mus musculus OX=10090 GN=Mrps14 PE=1 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 6.947e-20
Identity = 40/107 (37.38%), Postives = 69/107 (64.49%), Query Frame = 2 
Query:  245 FPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGV 565
             PP         +++ R++RD K+R +++ +   R R+ +L KN ++P++++++A  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+ L SGV
Sbjct:   17 VPPSASGQVRGYYVDWRMLRDLKRRKMAYEYADERLRINSLRKNTILPKDLQEMAGDEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGLLSGV 123
[Close]
BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|Q6B860|RT14_BOVIN (28S ribosomal protein S14, mitochondrial OS=Bos taurus OX=9913 GN=MRPS14 PE=1 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 1.733e-18
Identity = 37/98 (37.76%), Postives = 67/98 (68.37%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            +++ R++RD K+R +++ +   R R+ +L KN ++P+++++VA  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+   SG+ + 
Sbjct:   29 YVDWRMLRDVKRRKMAYEYADERLRINSLRKNTILPKHLQEVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGIQRA 126
[Close]
BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|O60783|RT14_HUMAN (28S ribosomal protein S14, mitochondrial OS=Homo sapiens OX=9606 GN=MRPS14 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.174e-17
Identity = 37/100 (37.00%), Postives = 65/100 (65.00%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            +++ R+ RD K+R +++ +   R R+ +L KN ++P+ ++ VA  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+   SG+ +  +
Sbjct:   29 YVDWRMWRDVKRRKMAYEYADERLRINSLRKNTILPKILQDVADEEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHGQLSGIQRATW 128
[Close]
BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|Q5HAT5|RS14_EHRRW (30S ribosomal protein S14 OS=Ehrlichia ruminantium (strain Welgevonden) OX=254945 GN=rpsN PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 4.512e-10
Identity = 30/95 (31.58%), Postives = 56/95 (58.95%), Query Frame = 2 
Query:  296 IIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            I R+ K+  +    +  R +L A+ K++ +  N + +AQ +L +LPRDS++ R++  C++TGR RGC  ++ +SRI+ R         G+ K ++
Sbjct:    7 IQRNLKRISICDRLKSKREKLRAIIKDQSISMNDRFLAQVKLSKLPRDSSYIRIRNRCLITGRPRGCYRKFKVSRIVLRQLGSIGQIPGLTKSSW 101
[Close]
BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Match: sp|Q3YRM2|RS14_EHRCJ (30S ribosomal protein S14 OS=Ehrlichia canis (strain Jake) OX=269484 GN=rpsN PE=3 SV=1)

HSP 1 Score: 58.9214 bits (141), Expect = 4.794e-10
Identity = 30/95 (31.58%), Postives = 55/95 (57.89%), Query Frame = 2 
Query:  296 IIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            I R+ K+  +    +  R  L A+ K++ +  N + +AQ +L +LPRDS++ R++  C++TGR RGC  ++ +SRI+ R         G+ K ++
Sbjct:    7 IQRNLKRISICSRLKSKRDELKAIIKDQSISMNDRFLAQVKLSKLPRDSSYIRIRNRCLVTGRPRGCYRKFKVSRIVLRQLGSIGQIPGLTKSSW 101
[Close]
BLAST of 28S ribosomal protein S14 vs. TrEMBL
Match: A0A183KKJ6 (Uncharacterized protein OS=Schistosoma curassoni OX=6186 GN=SCUD_LOCUS15558 PE=4 SV=1)

HSP 1 Score: 192.586 bits (488), Expect = 1.181e-57
Identity = 92/183 (50.27%), Postives = 123/183 (67.21%), Query Frame = 2 
Query:  134 PEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADD 682
            P +  E++   +    KTP HHNN E EKLL+ ELC +P Y+     HGF +A +IRD+K+R VS HF  +R RL A+ KN ++P+ IK +A S +  LPRDSNWTR+   CI+T RRRGCKTRW +SRI+WR +ADYN  SGV+   +   T+  R+ M+WPPP + PV EYI+RY+ N  D
Sbjct:   31 PLIPSEILYQREITPAKTPAHHNNSEIEKLLKSELCDYPAYSSRKIRHGFQDAIMIRDYKRRQVSAHFCNLRIRLNAIRKNTILPQEIKDLATSYISSLPRDSNWTRILSRCIVTSRRRGCKTRWKVSRIVWRKYADYNRMSGVLWARWGSNTRSVRRHMLWPPPRETPVSEYIRRYYKNLAD 213
[Close]
BLAST of 28S ribosomal protein S14 vs. TrEMBL
Match: A0A430PXT2 (Small subunit ribosomal protein S14 OS=Schistosoma bovis OX=6184 GN=DC041_0002026 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 3.498e-57
Identity = 92/183 (50.27%), Postives = 122/183 (66.67%), Query Frame = 2 
Query:  134 PEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADD 682
            P +  E++   +    KTP HHNN E EKLL+ ELC +P Y+     HGF +A +IRD+K+R VS HF  +R RL A+ KN ++P+ IK +A S +  LPRDSNWTR+   CI+T RRRGCKTRW +SRI+WR +ADYN  SGV+   +   T+  R+ M+WPPP + PV EYI RY+ N  D
Sbjct:   31 PLIPSEILYQREITPAKTPAHHNNSEIEKLLKSELCDYPAYSSRKIRHGFQDAIMIRDYKRRQVSAHFCNLRIRLNAIRKNTILPQEIKDLATSYISSLPRDSNWTRILSRCIVTSRRRGCKTRWKVSRIVWRKYADYNRMSGVLWARWGSNTRSVRRHMLWPPPRETPVSEYIHRYYKNLAD 213
[Close]
BLAST of 28S ribosomal protein S14 vs. TrEMBL
Match: A0A095BZW4 (28S ribosomal protein S14, mitochondrial OS=Schistosoma haematobium OX=6185 GN=MS3_02972 PE=4 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 3.653e-57
Identity = 92/183 (50.27%), Postives = 122/183 (66.67%), Query Frame = 2 
Query:  134 PEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADD 682
            P +  E++   +    KTP HHNN E EKLL+ ELC +P Y+     HGF +A +IRD+K+R VS HF  +R RL A+ KN ++P+ IK +A S +  LPRDSNWTR+   CI+T RRRGCKTRW +SRI+WR +ADYN  SGV+   +   T+  R+ M+WPPP + PV EYI RY+ N  D
Sbjct:   31 PLIPSEILYQREITPAKTPAHHNNSEIEKLLKSELCDYPAYSSRKIRHGFQDAIMIRDYKRRQVSAHFCNLRIRLNAIRKNTILPQEIKDLATSYISSLPRDSNWTRILSRCIVTSRRRGCKTRWKVSRIVWRKYADYNRMSGVLWARWGSNTRSVRRHMLWPPPRETPVSEYIHRYYKNLAD 213
[Close]
BLAST of 28S ribosomal protein S14 vs. TrEMBL
Match: A0A183MSJ7 (Uncharacterized protein OS=Schistosoma margrebowiei OX=48269 GN=SMRZ_LOCUS19022 PE=4 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 1.599e-56
Identity = 91/183 (49.73%), Postives = 122/183 (66.67%), Query Frame = 2 
Query:  134 PEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADD 682
            P +  E++   +    KTP HHNN E EKLL+ ELC +P Y+     HGF +A +IRD+K+R VS HF  +R RL A+ KN ++P+ IK +A   +  LPRDSNWTR+   CI+T RRRGCKTRW +SRI+WR +ADYN  SGV+   +   T+  R+ M+WPPP + PV EYI+RY+ N  D
Sbjct:   31 PLIPSEILYQREITPAKTPAHHNNSEIEKLLKNELCDYPAYSSRKIRHGFQDAIMIRDYKRRQVSAHFCNLRIRLNAIRKNTILPQEIKDLATLYISSLPRDSNWTRILSRCIVTSRRRGCKTRWKVSRIVWRKYADYNRMSGVLWARWGSNTRSVRRHMLWPPPRETPVSEYIRRYYKNLAD 213
[Close]
BLAST of 28S ribosomal protein S14 vs. TrEMBL
Match: A0A183P5Q2 (Uncharacterized protein OS=Schistosoma mattheei OX=31246 GN=SMTD_LOCUS9688 PE=4 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 3.067e-56
Identity = 91/183 (49.73%), Postives = 122/183 (66.67%), Query Frame = 2 
Query:  134 PEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADD 682
            P +  E++   +    KTP HH+N E EKLL+ ELC +P Y+     HGF +A +IRD+K+R VS HF  +R RL A+ KN ++P+ IK +A S +  LPRDSNWTR+   CI+T RRRGCKTRW +SRI+WR +ADYN  SGV+   +   T+  R+ M+WPPP + PV EYI RY+ N  D
Sbjct:   31 PLIPSEILYQREITPTKTPAHHHNSEIEKLLKSELCDYPAYSSRKIRHGFQDAIMIRDYKRRQVSAHFCNLRIRLNAIRKNTILPQEIKDLATSYISSLPRDSNWTRILSRCIVTSRRRGCKTRWRVSRIVWRKYADYNRMSGVLWARWGSNTRSVRRHMLWPPPRETPVSEYIHRYYKNLAD 213
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Cavefish
Match: mrps14 (mitochondrial ribosomal protein S14 [Source:NCBI gene;Acc:111194255])

HSP 1 Score: 85.8853 bits (211), Expect = 9.982e-21
Identity = 39/98 (39.80%), Postives = 67/98 (68.37%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            +++ R+++D K+R +++ F   R R+ A+ KN ++P+ +++VA  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+N  SGV + 
Sbjct:   34 YVDWRMLKDVKRRQMAYDFSDTRLRINAVRKNTVLPKELQEVADKEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRVSRIVFRHLADHNQMSGVQRA 131
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Sea Lamprey
Match: MRPS14 (mitochondrial ribosomal protein S14 [Source:HGNC Symbol;Acc:HGNC:14049])

HSP 1 Score: 77.0258 bits (188), Expect = 4.856e-18
Identity = 38/101 (37.62%), Postives = 65/101 (64.36%), Query Frame = 2 
Query:  272 SHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            SH +++ R++RD K+R+++  +   R R+ AL KN ++P+ +++ A  ++  LPRDS   R+   C+MT R RG K RW +SRI++R  AD+   SG+ + 
Sbjct:   37 SH-YVDWRMLRDVKRRMMAAEYTDERLRINALRKNTILPKELQEEADKEIAALPRDSCPVRIHNRCVMTSRPRGVKRRWRLSRIVFRDLADHGQMSGIQRA 136
[Close]
BLAST of 28S ribosomal protein S14 vs. Ensembl Yeast
Match: MRP2 (Mitochondrial ribosomal protein of the small subunit [Source:SGD;Acc:S000006370])

HSP 1 Score: 41.9726 bits (97), Expect = 9.726e-6
Identity = 32/99 (32.32%), Postives = 48/99 (48.48%), Query Frame = 2 
Query:  278 GFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKG 574
            GFINARI+RD  KR      E +   L  + +N  +P  ++  AQ +L  LP     T++K  C+ +G  R   + + + R  +R  A      GV KG
Sbjct:   15 GFINARILRDNFKRQQFKENEILVKSLKFIARNMNLPTKLRLEAQLKLNALPNYMRSTQIKNRCVDSGHARFVLSDFRLCRYQFRENALKGNLPGVKKG 113
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BLAST of 28S ribosomal protein S14 vs. Ensembl Nematostella
Match: EDO49092 (Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7RI50])

HSP 1 Score: 65.0846 bits (157), Expect = 1.408e-13
Identity = 33/93 (35.48%), Postives = 59/93 (63.44%), Query Frame = 2 
Query:  302 RDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            RD K+R +   FE  R+ L  + K+ ++P  ++K A ++++ LP +S+ TR++  C+MT R RG   R+ +SR+M+R+FA+    +GV K  +
Sbjct:   28 RDIKRREMFKDFEQQRSALRMIKKSDILPSGVRKNAANEMKELPLNSSLTRIRNRCVMTDRGRGIVGRFRVSRMMFRYFAERGQIAGVKKAQW 120
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BLAST of 28S ribosomal protein S14 vs. Ensembl Medaka
Match: mrps14 (mitochondrial ribosomal protein S14 [Source:NCBI gene;Acc:101155884])

HSP 1 Score: 83.1889 bits (204), Expect = 1.017e-19
Identity = 40/100 (40.00%), Postives = 68/100 (68.00%), Query Frame = 2 
Query:  281 FINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNY 580
            +++ R++RD K+R ++F +   R R+ AL KN ++P+ ++ VA  ++  LPRDS   R++  C+MT R RG K RW +SRI++RH AD+N  SG+++  +
Sbjct:   40 YVDWRMLRDVKRRQMAFDYADERLRINALRKNTILPKELQ-VADKEIAALPRDSCPVRIRNRCVMTSRPRGVKRRWRLSRIVFRHLADHNQMSGILRARW 138
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BLAST of 28S ribosomal protein S14 vs. Planmine SMEST
Match: SMESG000066757.1 (SMESG000066757.1)

HSP 1 Score: 493.041 bits (1268), Expect = 1.976e-179
Identity = 238/238 (100.00%), Postives = 238/238 (100.00%), Query Frame = 2 
Query:   26 MLKNLSNVFSIINSSFLKLGINEYSLAKFSTNFPKWPEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADDSPAESSEFNLKVLSIKDSK 739
            MLKNLSNVFSIINSSFLKLGINEYSLAKFSTNFPKWPEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADDSPAESSEFNLKVLSIKDSK
Sbjct:    1 MLKNLSNVFSIINSSFLKLGINEYSLAKFSTNFPKWPEVYDELILPLDRGFRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLVSFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIMTGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPPPNDIPVGEYIKRYHDNADDSPAESSEFNLKVLSIKDSK 238
The following BLAST results are available for this feature:
BLAST of 28S ribosomal protein S14 vs. Ensembl Human
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Human e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPS148.692e-1837.00mitochondrial ribosomal protein S14 [Source:HGNC S... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Celegans
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Celegan e!99)
Total hits: 0
Match NameE-valueIdentityDescription
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BLAST of 28S ribosomal protein S14 vs. Ensembl Fly
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Drosophila e!99)
Total hits: 2
Match NameE-valueIdentityDescription
mRpS149.296e-1640.66gene:FBgn0044030 transcript:FBtr0074785[more]
mRpS149.296e-1640.66gene:FBgn0044030 transcript:FBtr0346015[more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Zebrafish
Analysis Date: 2016-08-08 (Schmidtea mediterranea smed_20140614 BLASTX Zebrafish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps143.191e-1933.63mitochondrial ribosomal protein S14 [Source:ZFIN;A... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Xenopus
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Xenopus e!99)
Total hits: 2
Match NameE-valueIdentityDescription
garnl34.808e-1736.73GTPase activating Rap/RanGAP domain-like 3 [Source... [more]
garnl31.059e-1636.73GTPase activating Rap/RanGAP domain-like 3 [Source... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Mouse
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX Mouse e!99)
Total hits: 2
Match NameE-valueIdentityDescription
Mrps141.608e-2137.50mitochondrial ribosomal protein S14 [Source:MGI Sy... [more]
Mrps149.932e-2137.38mitochondrial ribosomal protein S14 [Source:MGI Sy... [more]
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BLAST of 28S ribosomal protein S14 vs. UniProt/SwissProt
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI UniProt)
Total hits: 5
Match NameE-valueIdentityDescription
sp|Q9CR88|RT14_MOUSE6.947e-2037.3828S ribosomal protein S14, mitochondrial OS=Mus mu... [more]
sp|Q6B860|RT14_BOVIN1.733e-1837.7628S ribosomal protein S14, mitochondrial OS=Bos ta... [more]
sp|O60783|RT14_HUMAN4.174e-1737.0028S ribosomal protein S14, mitochondrial OS=Homo s... [more]
sp|Q5HAT5|RS14_EHRRW4.512e-1031.5830S ribosomal protein S14 OS=Ehrlichia ruminantium... [more]
sp|Q3YRM2|RS14_EHRCJ4.794e-1031.5830S ribosomal protein S14 OS=Ehrlichia canis (stra... [more]
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BLAST of 28S ribosomal protein S14 vs. TrEMBL
Analysis Date: 2020-05-01 (Schmidtea mediterranea smed_20140614 BLASTX EMBL-EBI TrEMBL)
Total hits: 5
Match NameE-valueIdentityDescription
A0A183KKJ61.181e-5750.27Uncharacterized protein OS=Schistosoma curassoni O... [more]
A0A430PXT23.498e-5750.27Small subunit ribosomal protein S14 OS=Schistosoma... [more]
A0A095BZW43.653e-5750.2728S ribosomal protein S14, mitochondrial OS=Schist... [more]
A0A183MSJ71.599e-5649.73Uncharacterized protein OS=Schistosoma margrebowie... [more]
A0A183P5Q23.067e-5649.73Uncharacterized protein OS=Schistosoma mattheei OX... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Cavefish
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Cavefish e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps149.982e-2139.80mitochondrial ribosomal protein S14 [Source:NCBI g... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Sea Lamprey
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Sea Lamprey e!99)
Total hits: 1
Match NameE-valueIdentityDescription
MRPS144.856e-1837.62mitochondrial ribosomal protein S14 [Source:HGNC S... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Yeast
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Yeast e!Fungi46)
Total hits: 1
Match NameE-valueIdentityDescription
MRP29.726e-632.32Mitochondrial ribosomal protein of the small subun... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Nematostella
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Nematostella e!Metazoa46)
Total hits: 1
Match NameE-valueIdentityDescription
EDO490921.408e-1335.48Predicted protein [Source:UniProtKB/TrEMBL;Acc:A7... [more]
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BLAST of 28S ribosomal protein S14 vs. Ensembl Medaka
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Medaka e!99)
Total hits: 1
Match NameE-valueIdentityDescription
mrps141.017e-1940.00mitochondrial ribosomal protein S14 [Source:NCBI g... [more]
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BLAST of 28S ribosomal protein S14 vs. Planmine SMEST
Analysis Date: 2020-05-08 (Schmidtea mediterranea smed_20140614 BLASTX Planmine SMEST)
Total hits: 1
Match NameE-valueIdentityDescription
SMESG000066757.11.976e-179100.00SMESG000066757.1[more]
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Sequences
The following sequences are available for this feature:

transcript sequence

>SMED30010291 ID=SMED30010291|Name=28S ribosomal protein S14|organism=Schmidtea mediterranea sexual|type=transcript|length=793bp
GTTTTAATAATCAATTTTGAGATCAATGCTTAAAAATTTGTCAAACGTAT
TTTCTATTATAAATTCCAGTTTCTTAAAATTAGGTATCAATGAGTATTCC
TTAGCTAAATTTTCAACTAATTTTCCAAAATGGCCAGAAGTTTACGATGA
ATTAATATTACCTCTTGATAGAGGTTTTAGAAAAACTCCTGAACATCATA
ACAATCAAGAAACAGAAAAACTTTTGGAAAAAGAACTTTGCCGATTCCCT
CCATATAATCAACCATATTATTCCCATGGTTTTATAAATGCAAGAATCAT
TCGTGATTGGAAAAAGCGATTGGTTTCTTTTCATTTTGAACCTGTAAGAA
CTCGATTGATAGCGCTATTTAAGAATAAGTTAATGCCGGAAAATATTAAA
AAAGTAGCACAAAGTCAACTTGAACGACTTCCCAGAGATTCTAATTGGAC
CAGAGTAAAAGTATTTTGTATTATGACTGGTAGGAGGCGAGGATGTAAAA
CTCGATGGGGAATAAGTCGTATAATGTGGCGTCATTTTGCAGATTACAAT
CTAGCTAGTGGGGTCATCAAAGGAAATTATTTATTCAGAACTAAAGAAGA
ACGAAAGTTAATGATTTGGCCGCCTCCTAATGATATTCCCGTTGGAGAAT
ATATCAAACGTTATCATGATAATGCTGATGATTCGCCAGCAGAAAGTTCG
GAATTTAATTTAAAGGTTCTCAGTATCAAAGACTCAAAATAAATGCTCTG
TCTTGTGTTTTTTAATTAACAAATATTATATAACGCAGCCGAT
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protein sequence of SMED30010291-orf-1

>SMED30010291-orf-1 ID=SMED30010291-orf-1|Name=SMED30010291-orf-1|organism=Schmidtea mediterranea sexual|type=polypeptide|length=239bp
MLKNLSNVFSIINSSFLKLGINEYSLAKFSTNFPKWPEVYDELILPLDRG
FRKTPEHHNNQETEKLLEKELCRFPPYNQPYYSHGFINARIIRDWKKRLV
SFHFEPVRTRLIALFKNKLMPENIKKVAQSQLERLPRDSNWTRVKVFCIM
TGRRRGCKTRWGISRIMWRHFADYNLASGVIKGNYLFRTKEERKLMIWPP
PNDIPVGEYIKRYHDNADDSPAESSEFNLKVLSIKDSK*
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Annotated Terms
The following terms have been associated with this transcript:
Vocabulary: Planarian Anatomy
TermDefinition
PLANA:0000101muscle cell
PLANA:0000142posterior region of the whole animal
PLANA:0000429neoblast
PLANA:0002109X1 cell
Vocabulary: INTERPRO
TermDefinition
IPR043140Ribosomal_S14/S29
IPR001209Ribosomal_S14
Vocabulary: cellular component
TermDefinition
GO:0005840ribosome
GO:0005622intracellular
Vocabulary: biological process
TermDefinition
GO:0006412translation
Vocabulary: molecular function
TermDefinition
GO:0003735structural constituent of ribosome
InterPro
Analysis Name: Schmidtea mediteranean smed_20140614 Interproscan
Date Performed: 2020-05-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR043140Ribosomal protein S14/S29GENE3DG3DSA:4.10.830.10coord: 124..184
e-value: 1.3E-6
score: 29.9
IPR001209Ribosomal protein S14PFAMPF00253Ribosomal_S14coord: 131..182
e-value: 3.7E-14
score: 52.2
IPR001209Ribosomal protein S14PANTHERPTHR1983630S RIBOSOMAL PROTEIN S14coord: 85..182
NoneNo IPR availablePANTHERPTHR19836:SF1928S RIBOSOMAL PROTEIN S14, MITOCHONDRIALcoord: 85..182
NoneNo IPR availableSUPERFAMILYSSF57716Glucocorticoid receptor-like (DNA-binding domain)coord: 88..183